CN110721326A

CN110721326A - Manufacturing and application of multifunctional laboratory disinfection cleaning machine

Info

Publication number: CN110721326A
Application number: CN201910975681.6A
Authority: CN
Inventors: 吴洪生
Original assignee: Nanjing Yi Gang Eco Environment Technology Co Ltd
Current assignee: Nanjing Yi Gang Eco Environment Technology Co Ltd
Priority date: 2019-10-14
Filing date: 2019-10-14
Publication date: 2020-01-24

Abstract

The utility model provides a multi-functional disinfection cleaning machine in laboratory makes and uses, this equipment belongs to laboratory air disinfection, laboratory article disinfection and environmental protection field. The cleaning machine is composed of the following parts: air filter, dryer, air compressor, high-voltage electrode ozone discharger, programmable controller, frequency converter, transformer, control valve, delivery pump, water tank and various connectors and connecting pipelines. Ozone generated by the device enters a water tank of the cleaning machine, is dissolved in water, can quickly kill microorganisms, enzyme activity, nucleic acid, transgenic biological test materials and decompose toxic organic matters, and bubbles and vibrates to remove dirt on the surface of an object to be cleaned.

Description

Manufacturing and application of multifunctional laboratory disinfection cleaning machine

Technical Field

The invention belongs to the field of laboratory article disinfection, laboratory air disinfection and environmental protection, and particularly relates to manufacture and application of a laboratory multifunctional disinfection cleaning machine.

Background

The laboratory is the place of carrying out teaching demonstration and scientific research and scientific detection, often carries out a large amount of experimental analysis and detection, especially microorganism and biotechnology laboratory, carries out a large amount of experiments every day, produces a large amount of culture dishes, test tube, triangular flask, beaker, centrifuging tube etc. especially carries out transgene, molecular biology experiment, germ test, article after the experiment finishes to and carry out the relevant experimental apparatus of next test, need to carry out sanitization and then the sanitization, traditional this type of studio is separately gone on, very loaded down with trivial details.

The method for disinfecting and cleaning common utensils in the laboratory comprises the following steps:

physical disinfection method

1. Mechanical disinfection: the soap is generally used for scrubbing, the water is used for washing, most or even all bacteria on articles or utensils can be eliminated, the multi-layer mask can be used for preventing pathogens from being discharged or invaded from the respiratory tract, and the filter of the ventilation device can be used for keeping the air in an operating room, a laboratory and an isolation ward, so that the sterile state is protected.

2. Thermal disinfection: including fire, boiling, flowing steam, high-heat steam, dry heat sterilization, etc., can make pathogen protein solidify and denature, and lose normal metabolism function.

(1) Firing: the method can be used for pollutants with low economic value, metal instruments, corpses and the like, is simple, convenient, economic and stable in effect, (2) boiling: the method is used for sterilizing boiling-resistant articles and general metal instruments at 100 ℃ for 1-2 minutes, but the spores need a longer time, the spores of bacillus anthracis need to be boiled for 30 minutes, the spores of tetanus need to be boiled for 3 hours, the spores of clostridium botulinum need to be sterilized for 6 hours, the metal instruments are sterilized, 1-2% of sodium carbonate or 0.5% of soft soap and other alkaline agents are added to dissolve fat and enhance the sterilizing power, 1% of soapy water 151/kg is added to cotton fabrics, the sterilizing and decontamination effects are achieved, the articles can not exceed the volume 3/4 when being boiled and sterilized, and the articles are immersed under the water surface, and gaps are kept for facilitating convection.

(3) Sterilization by flowing steam: the relative humidity is 80-100%, the temperature is near 100 ℃, water vapor is condensed on the surface of an object to emit heat energy to kill pathogens, and when the vapor is condensed and contracted to generate negative pressure, the hot vapor on the outer layer is promoted to enter and be supplemented, the steam penetrates to the deep part of the object, the heat is accelerated, and the disinfection is promoted.

(4) High-pressure steam sterilization: usually, the pressure is 98.066kPa, the temperature is 121-126 ℃, bacterial spores can be completely killed within 15-20 minutes, and the method is suitable for heat-resistant and damp-proof articles.

(5) Dry heat sterilization: the dry hot air has poor conduction, small heat capacity, weak penetrating power and slow object heating, can be sterilized only after 1 to 2 hours at the temperature of 160 to 170 ℃, and is suitable for glass containers, metal instruments and the like which cannot carry water, and the heat tolerance of different pathogens is expressed by the heat death time.

3. Radiation disinfection: there are two types of non-ionizing radiation, ultraviolet, infrared and microwave, and the latter includes high-energy electron beams (cathode rays) of gamma rays. The ionizing radiation equipment is expensive and has certain damage to articles and human bodies, so the ionizing radiation equipment is less in use, at present, the ionizing radiation equipment is mostly ultraviolet rays, can cause the change of cell components, particularly nucleic acid, protoplasm protein and acid, to cause the death of microorganisms, the ultraviolet wavelength range is 2100 to 3280A, the wavelength for killing the microorganisms is 2000 to 3000A, the most powerful of 2500-2650A, the most powerful of fungal spores for ultraviolet ray tolerance, the most powerful of bacterial spores, the weakest of bacterial propagules, with few exceptions, the penetration force of ultraviolet ray is poor, the ordinary glass with the thickness of 2mm can not be penetrated by the glass below 3000A, the sterilization effect can be reduced by dust and relative humidity in the air, the penetration force to water is reduced along with the depth and turbidity, but the use is convenient, the medicine has no damage to medicine, so it is widely used for disinfecting air and general article surface, and can produce erythema, ultraviolet ophthalmia and ozone poisoning when irradiated on human body. Sunlight solarization also depends on ultraviolet rays in the sunlight, but because scattering and absorption in the atmospheric layer are used, only 39% of the sunlight can reach the ground, so the sunlight solarization is only suitable for microorganisms with low endurance, and long-time solarization is needed.

(II) chemical disinfection method

Based on their effect on pathogen proteins, they are classified into the following categories:

1. the disinfectant for coagulating protein comprises phenols, acids and alcohols

(1) Phenols: phenol, lisu, hexachlorophene and the like are mainly used, have special odor and limited bactericidal power, can discolor textiles, embrittle rubber articles and have certain irritation to skin, so that phenol (carbolic acid): colorless crystals, special odor, pink color when wetted, and unreduced toxicity, is original cell pulp toxicity, can kill bacteria reproduction type solution of 1: 80-1: 110 at 20 ℃ for 30 minutes, but can not kill spores and viruses with strong resistance, soap is added to saponify fat, dissolve protein, promote penetration, strengthen disinfection effect, but has high toxicity, irritation to skin, malodor, and can not be used for skin disinfection, Laussu (coal phenol soap) (lysol): the antibacterial agent is reddish brown, is easy to dissolve in water, has a decontamination effect, has 2-5 times stronger bactericidal power than phenol carbolic acid, is usually a 2-5% aqueous solution, can be used for spraying, wiping, soaking containers, washing hands and the like, can kill bacteria in a breeding type for 10-15 minutes, and has a poor effect on spores (hexochlorophorphine): the soap is a bisphenol compound, is slightly soluble in water, is easily soluble in alcohol, ester and ether, can promote dissolution by adding alkali or soap, has low toxicity and irritation, but has strong bactericidal power, is mainly used for skin disinfection, can reduce 80-90% of skin bacteria by washing hands with 2.5-3% of hexachlorophene soap, can generate nerve damage by reports, and is suitable for long-term use.

(2) Acids: the disinfectant has the effects of killing bacterial propagules and spores, but is easy to damage articles, so the disinfectant is not generally used for disinfecting living rooms, 5 percent hydrochloric acid can disinfect and wash tableware, fruits, 15 percent salt is added into 2.5 percent solution to disinfect fur and leather, 101/kg of the disinfectant is heated to 30 ℃ and soaked for 40 hours, lactic acid is commonly used for air disinfection, 10g of lactic acid is used for fumigating 30 minutes in a space of 100m3, and staphylococcus and influenza virus can be killed, (3) alcohol ethanol (alcohol): 75% concentration can quickly kill bacteria reproduction type, has slow action on general virus, has no definite action on hepatitis virus, has a certain action on fungus spore, and has no action on spore, and can be used for skin disinfection and thermometer soaking disinfection, and can not be used for soaking disinfection of surgical instruments because of not killing spore, and the bactericidal power of isopropyl alcohol (isopropyl alcohol) is greater than 2.

Mainly used as alkaline drugs, sodium hydroxide, lime and the like are commonly used: (1) sodium oxychloride is white crystal, is easy to dissolve in water, has strong bactericidal power, can kill virus and bacteria reproduction in 2-4% solution, can kill tubercle bacillus in 10% solution, can kill spore in 10 min in 30% solution, is very rarely used due to strong corrosivity, and is only used for killing bacillus anthracis. (2) Lime can generate high temperature when meeting water, dissolve protein and kill pathogens, 10-20% lime milk is commonly used for disinfecting excrement, the dosage of the lime milk is 2 times of that of the excrement, the lime milk is stirred and acted for 4-5 hours, the 20% lime milk is used for disinfecting a anthrax bacteria polluted place, the lime milk is sprayed once every 4-6 hours and is continuously sprayed for 2-3 times, and the lime milk can kill tubercle bacillus when being brushed for 2 times.

3. Oxidized protein disinfectant

The disinfectant comprises chlorine-containing disinfectant and peroxide disinfectant, and has strong sterilizing power, so the disinfectant is widely applied to medical and epidemic prevention work at present.

(1) The bleaching powder is most widely applied, the main component is calcium hypochlorite [ Ca (OCl)2], the bleaching powder contains 25-30% of effective components, the property is unstable, the bleaching powder can be decomposed by light, heat, moisture and CO2, therefore, the bleaching powder is sealed and stored in a dark dry place for no more than 1 year, the effective component, namely the hypochlorous acid can permeate into cells, oxidize the sulfhydryl gene of cell enzyme, destroy cytoplasm metabolism, the sterilization capability is strong and rapid in an acid environment, spores can be killed by high concentration, the powder is used for disinfecting excrement, phlegm, pus and the like, 200 g of dry powder is added into each liter of the powder, the stirring is uniform, the bleaching powder is placed for 1-2, 5 g of dry powder is added into each liter of urine, the urine is placed for 10 minutes, the 10-20% of emulsion can be used for spraying a toilet and a polluted vehicle and the like except disinfected excrement and secretion, the actual effective chlorine content is measured, the preparation dosage is corrected, the powder and the tablet of the bleaching powder can contain 60-, when in use, the dosage can be reduced in proportion.

(2) chloramine-T is an organic chlorine disinfectant, contains 24-26% of available chlorine, is stable in performance, is sealed and kept for 1 year, only loses 0.1% of the available chlorine, is slightly soluble in water (12%), is less in irritation and corrosivity, has slower action than hypochlorous acid, can kill bacteria reproduction in 0.2% 1 hour, can kill mycobacterium tuberculosis in 5% 2 hours, and can kill spores in more than 10 hours, various ammonium salts can promote the sterilization effect of the chloramine-T, and a 1-2.5% solution also has an effect on hepatitis viruses.

(3) Sodium dichloroisocyanurate, also named as sodium monochloride, is a widely used organic chlorine disinfectant, contains 60-64.5% of chlorine, and has the advantages of high efficiency, broad spectrum, stability, high solubility, low toxicity, etc. the aqueous solution can be used for spraying, soaking and wiping foam, and can also be used for directly disinfecting pollutants by using dry powder to treat excrement and the like, the aqueous solution and the bleaching powder are directly sprayed on the ground, the dosage is 10-20 g/m < 2 >, the aqueous solution and the paraformaldehyde dry powder are mixed and ignited, the gas can be fumigated and disinfected, and can be mixed with No. 92 coagulant (synthesized by adding iron powder, sulfuric acid, hydrogen peroxide and the like on the basis of aluminum hydroxychloride) in a ratio of 1: 4 to form 'water-meeting clear' for drinking water disinfection, and can be prepared into various disinfection washing liquids with sodium sulfonate, such as cleaning, and the like, and has the same effects on hepatitis viruses.

(4) The peroxyacetic acid, also known as peroxyacetic acid, is a colorless transparent liquid, is volatile, has irritant sour taste, is a quick-acting disinfectant with the same effect, is easy to dissolve in organic solvents such as water and ethanol, has a bleaching corrosion effect, is volatile irritant sour taste, is a high-efficiency quick-acting disinfectant, is easy to dissolve in organic solvents such as water and ethanol, has bleaching and corrosion effects, is unstable in nature, can be easily decomposed by heat, organic matters, heavy metal ions, strong alkali and the like, can kill bacterial propagules within 0.01-0.5%, can kill spores within 0.5-10 minutes, can kill spores within 1-5 minutes, has a common concentration of 0.5-2%, can be disinfected by soaking, spraying, wiping and the like, and is disinfected by aerosol (with the concentration of 5%, 2.5ml/m2) and fumigation (with the concentration of 0.75-1.0 g/m3) under a closed condition.

The methods have been used for years and have respective advantages and disadvantages, but the common points are that the methods have harm to people, have disinfection and sterilization effects, have no cleaning effect and cannot simultaneously remove toxic organic matters.

Ozone is a substance with strong oxidizability, can kill various microorganisms, thoroughly oxidize and decompose organic molecules into carbon dioxide and water, and turn into oxygen without secondary pollution.

The laboratory multifunctional disinfection cleaning machine is designed by adopting a mechanism of generating high-concentration ozone through high-voltage discharge, various microorganism and biological test material residues are quickly killed by using strong oxidizing substances such as ozone and hydroxyl radicals contained in ozone water, biological test materials and organic dirt are quickly oxidized and decomposed, and meanwhile, bubbling oscillation is carried out to vibrate the dirt on the surface of an object and transfer the dirt into water, so that the dirt is oxidized and decomposed into carbon dioxide and water to be removed; meanwhile, the cleaning machine can also provide ozone gas for laboratories and operation platforms for disinfection and sterilization, which is more advanced than the current related reports.

Disclosure of Invention

The technical problem to be solved is as follows: aiming at the technical defects, the invention develops a laboratory multifunctional disinfection cleaning machine which can disinfect, sterilize and clean, is used for killing germs, enzyme activity, DNA and RNA, toxic organic dirt, organic toxicant and the like on test materials and test articles, and is cleaned.

The technical scheme is as follows: a laboratory multifunctional disinfection cleaning machine is manufactured and applied, and the steps are as follows: a. a square container with the side length of 45-100cm and the height of 50-150cm, and the periphery of the container is composed of a metal or plastic box body; an air filter, a dryer, an air compressor, a high-voltage electrode discharge ozonizer, a delivery pump and the like are sequentially arranged at the bottom of the box body of the square container; the devices are connected by pipelines, the gas outlet pipeline of the high-voltage electrode discharge ozonizer is connected with a delivery pump by a three-way valve, and the other end of the three-way valve is provided with a valve; the whole system is powered by a power supply system, the power supply adopts commercial power, a PLC programmable controller is connected beside the power supply, the whole system is regulated and controlled, a frequency converter and a step-up transformer are connected beside the power supply to change the frequency and the voltage of alternating current and control the ozone output speed, ozone is output by a delivery pump and enters a circular or square water tank with the volume of 5-150 liters to be mixed with water, the ozone dissolved in the water can quickly kill microorganisms and enzyme activity on an article to be cleared, organic substance dirt and toxic organic substances attached to the article are oxidized and decomposed into carbon dioxide and water, nucleic acid is thoroughly decomposed, and the dirt on the article is transferred into an aqueous solution to be oxidized and decomposed for removal through bubbling and oscillation; b. after the unit devices in the step a are assembled, a power supply is switched on, an air compressor is started, particulate matter impurities in air are filtered by the air through a filter, the air enters a dryer, is dried by the dryer to remove moisture, enters the air compressor to generate high-pressure compressed air flow, enters a high-pressure electrode discharge ozonizer (consisting of 5-20 pairs of blocking dielectric electrodes), generates high-concentration ozone, is connected with a delivery pump through a three-way valve, starts the delivery pump to deliver the ozone into water in a water tank, is uniformly mixed in the water through bubbling oscillation, simultaneously oscillates and drops dirt and toxic substances on an object to be disinfected to carry out water treatment, generates Fenton reaction and free radical reaction, generates hydroxyl free radicals and the like, and increases the oxidizing power and the sterilizing power; c. in the b treatment process, automatic control can be realized by adjusting a programmable controller in a control panel, the frequency of alternating current is changed by a frequency converter and is increased from 50Hz to 1200Hz, the yield of ozone is improved, the alternating voltage is changed by a transformer, the voltage is increased from 220V to 15000V, the high-voltage discharge frequency and efficiency are improved, and the yield and the effect of ozone are improved. After the whole process, the last effluent of the step b contains active oxygen such as ozone, hydroxyl free radical and the like, the articles to be disinfected and cleaned are put into water in advance, then the ozone and the free radicals conveyed into the water by the conveying pump can quickly kill microorganisms on the articles to be disinfected and sterilized in a laboratory, biological test materials and molecular biological test materials are inactivated, enzyme activity is killed, DNA and RNA in the biological test materials are decomposed, toxic and harmful small molecular organic compounds on the articles (such as a culture dish, a test tube, a triangular flask, a centrifuge tube, biological tissues, test residual microbial preparations, plant and animal test materials and the like) are thoroughly oxidized into water and carbon dioxide, organic poisons such as organic pesticides, antibiotics and the like attached to the wall of the article are thoroughly decomposed into water and carbon dioxide, in addition, the ozone gas continuously bubbles into a water tank and continuously vibrates to accelerate dirt attached to the article to be separated into the water solution, continuously inflating, disinfecting, sterilizing and cleaning for more than 30 minutes to achieve good disinfection effect and cleaning effect, turning off a power supply, pouring a water tank solution, and taking out the disinfected and cleaned objects; in addition, the equipment can directly carry out air sterilization through the valve 12, such as air sterilization, aseptic room sterilization, super clean bench sterilization and the like; d. in the original components of the equipment, the air filter adopts rock wool, a filter screen, a cloth bag and other modes for air filtration; adding active carbon, anhydrous calcium sulfate, anhydrous copper sulfate, etc. into the dryer for dehydration and drying; the distance between the electrodes of the high-voltage electrode discharge ozonizer is 0.5-4mm, so that the ozone generating effect is good; in order to improve the bactericidal effect and the oxidizing power of the ozone water, 0.1-1 g of ferrous sulfate can be added into the water to promote the Fenton reaction and generate more free radicals;

development of any one of the laboratory multifunctional disinfection and cleaning machines, and application of the laboratory multifunctional disinfection and cleaning machines in disinfection and cleaning of articles in microbiological laboratories, biological laboratories, botany laboratories, zoology laboratories, molecular biology laboratories, transgenic laboratories, physiological and biochemical laboratories, and air disinfection in laboratories.

Has the advantages that: the cleaning machine can rapidly remove various microorganisms, particularly pathogenic bacteria, enzyme activity, organic dirt, toxic organic substances and nucleic acid in test materials and test biological material residues to the maximum extent, can also sterilize and disinfect air and an operation table in a laboratory, ensures test and life safety, and cleans articles and test environments.

Compared with the prior art, the invention has the advantages that:

1) according to the invention, ozone is dissolved in water to generate ozone water, and ozone and free radical sterilization, disinfection and oxidation technologies are utilized, so that the method is simple and convenient, the technology is simple and easy to implement, the operability is strong, the cost is low, and the practicability is strong;

2) the ozone water-ozone technology of the therapeutic apparatus adopted by the invention can simultaneously eliminate pathogenic bacteria, enzyme activity, accounting and harmful and toxic organic chemical substances of test biological materials, test articles and the like, compared with the current common single chemical treatment, the introduced secondary pollutants, residues and secondary pollutants can be almost ignored because the ozone water is finally changed into water and oxygen.

Drawings

FIG. 1 is a comparison of results of processing using the present technique.

Detailed Description

The invention is further described below with reference to examples:

example 1:

1. the square container is 45cm in side length and 150cm in height, and the periphery of the square container is formed by a metal or plastic box body; an air filter, a dryer, an air compressor, a high-voltage electrode discharge ozonizer, a delivery pump and the like are sequentially arranged at the bottom of the box body of the square container; the devices are connected by pipelines, the gas outlet pipeline of the high-voltage electrode discharge ozonizer is connected with a delivery pump by a three-way valve, and the other end of the three-way valve is provided with a valve; the whole system is powered by a power supply system, the power supply adopts commercial power, a PLC (programmable logic controller) is connected beside the power supply, the whole system is regulated and controlled, a frequency converter and a step-up transformer are connected beside the power supply to change the frequency and the voltage of alternating current and control the ozone output speed, ozone is output by a delivery pump and enters a circular or square water tank with the volume of 5 liters to be mixed with water, the ozone dissolved in the water can quickly kill microorganisms and enzyme activity on an article to be clarified, organic substance dirt and toxic organic substances attached to the article are oxidized and decomposed into carbon dioxide and water, nucleic acid is thoroughly decomposed, and the dirt on the article is transferred into an aqueous solution to be oxidized, decomposed and removed by bubbling and oscillation;

2. after the unit devices in the step 1 are assembled, a power supply is switched on, an air compressor is started, particulate matter impurities in air are filtered by the air through a filter, the air enters a dryer, is dried by the dryer to remove moisture, enters the air compressor to generate high-pressure compressed air flow, enters a high-pressure electrode discharge ozonizer (consisting of 5-20 pairs of blocking dielectric electrodes), generates high-concentration ozone, is connected with a delivery pump through a three-way valve, starts the delivery pump to deliver the ozone into water in a water tank, is uniformly mixed in the water through bubbling oscillation, simultaneously oscillates and drops dirt and toxic substances on an object to be disinfected to carry out water treatment, generates fenton reaction and free radical reaction, generates hydroxyl free radicals and the like, and increases the oxidizing power and the sterilizing power;

3. in the above 2 processes, can be through adjusting the programmable controller in the control panel and realizing automatic control, change the alternating current frequency through the converter, rise from 50Hz to 1200Hz, improve the yield of ozone, change alternating voltage through the transformer, rise voltage from 220V to 15000V, improve high-voltage discharge frequency and efficiency, improve ozone yield and effect. After the whole process, the water discharged from the 2 last step contains active oxygen such as ozone, hydroxyl free radical and the like, the water is put into an object to be disinfected and cleaned in advance, then the ozone and the free radical conveyed into the water by the conveying pump can quickly kill microorganisms on the object to be disinfected and sterilized in a laboratory, biological test materials and molecular biological test materials are inactivated, enzyme activity is killed, DNA and RNA in the biological test materials are decomposed, toxic and harmful small molecular organic compounds on the object (a culture dish, a test tube, a triangular flask, a centrifuge tube, biological tissues, residual microbial preparations for tests, plant and animal test materials and the like) are thoroughly oxidized into water and carbon dioxide, organic poisons such as organic pesticides, antibiotics and the like attached to the wall of the object are thoroughly decomposed into water and carbon dioxide, in addition, the ozone gas continuously bubbles into a water tank and continuously vibrates to accelerate dirt attached to the object to separate into the water solution, continuously inflating, disinfecting, sterilizing and cleaning for more than 30 minutes to achieve good disinfection effect and cleaning effect, breaking DNA in the test watermelon plant leaf residues, ensuring that the enzyme activities of protease, DNA enzyme, catalase, peroxidase, superoxide dismutase and the like are almost zero, ensuring that the total number of bacteria is less than 18/g and the total number of fungi is less than 5/g, turning off a power supply, pouring a water tank solution, and taking out disinfected and cleaned objects; in addition, the equipment can directly carry out air sterilization through the valve 12, such as air sterilization, aseptic room sterilization, super clean bench sterilization and the like;

4. in the original components of the equipment, the air filter adopts rock wool, a filter screen, a cloth bag and other modes for air filtration; adding active carbon, anhydrous calcium sulfate, anhydrous copper sulfate, etc. into the dryer for dehydration and drying; the distance between the electrodes of the high-voltage electrode discharge ozonizer is 0.5mm, so that the ozone generating effect is good; in order to improve the bactericidal effect and the oxidizing power of the ozone water, 0.5 g of ferrous sulfate can be added into the water to promote the Fenton reaction and generate more free radicals;

example 2:

1. the square container with the side length of 60cm and the height of 120cm is formed by a metal or plastic box body on the periphery; an air filter, a dryer, an air compressor, a high-voltage electrode discharge ozonizer, a delivery pump and the like are sequentially arranged at the bottom of the box body of the square container; the devices are connected by pipelines, the gas outlet pipeline of the high-voltage electrode discharge ozonizer is connected with a delivery pump by a three-way valve, and the other end of the three-way valve is provided with a valve; the whole system is powered by a power supply system, the power supply adopts commercial power, a PLC (programmable logic controller) is connected beside the power supply, the whole system is regulated and controlled, a frequency converter and a step-up transformer are connected beside the power supply to change the frequency and the voltage of alternating current and control the ozone output speed, ozone is output by a delivery pump and enters a circular or square water tank with the volume of 5 liters to be mixed with water, the ozone dissolved in the water can quickly kill microorganisms and enzyme activity on an article to be clarified, organic substance dirt and toxic organic substances attached to the article are oxidized and decomposed into carbon dioxide and water, nucleic acid is thoroughly decomposed, and the dirt on the article is transferred into an aqueous solution to be oxidized, decomposed and removed by bubbling and oscillation;

3. in the above 2 processes, can be through adjusting the programmable controller in the control panel and realizing automatic control, change the alternating current frequency through the converter, rise from 50Hz to 1200Hz, improve the yield of ozone, change alternating voltage through the transformer, rise voltage from 220V to 15000V, improve high-voltage discharge frequency and efficiency, improve ozone yield and effect. After the whole process, the water discharged from the 2 last step contains active oxygen such as ozone, hydroxyl free radical and the like, the water is put into an object to be disinfected and cleaned in advance, then the ozone and the free radical conveyed into the water by the conveying pump can quickly kill microorganisms on the object to be disinfected and sterilized in a laboratory, biological test materials and molecular biological test materials are inactivated, enzyme activity is killed, DNA and RNA in the biological test materials are decomposed, toxic and harmful small molecular organic compounds on the object (a culture dish, a test tube, a triangular flask, a centrifuge tube, biological tissues, residual microbial preparations for tests, plant and animal test materials and the like) are thoroughly oxidized into water and carbon dioxide, organic poisons such as organic pesticides, antibiotics and the like attached to the wall of the object are thoroughly decomposed into water and carbon dioxide, in addition, the ozone gas continuously bubbles into a water tank and continuously vibrates to accelerate dirt attached to the object to separate into the water solution, continuously inflating, disinfecting, sterilizing and cleaning for more than 30 minutes to achieve good disinfection effect and cleaning effect, breaking DNA in the test watermelon plant leaf residues, ensuring that the enzyme activities of protease, DNA enzyme, catalase, peroxidase, superoxide dismutase and the like are almost zero, ensuring that the total number of bacteria is less than 23/g and the total number of fungi is less than 8/g, turning off a power supply, pouring a water tank solution, and taking out the disinfected and cleaned objects; in addition, the equipment can directly carry out air sterilization through the valve 12, such as air sterilization, aseptic room sterilization, super clean bench sterilization and the like;

4. in the original components of the equipment, the air filter adopts rock wool, a filter screen, a cloth bag and other modes for air filtration; adding active carbon, anhydrous calcium sulfate, anhydrous copper sulfate, etc. into the dryer for dehydration and drying; the distance between the electrodes of the high-voltage electrode discharge ozonizer is 0.5mm, so that the ozone generating effect is good; in order to improve the bactericidal effect and the oxidizing power of the ozone water, 0.1 g of ferrous sulfate can be added into the water to promote the Fenton reaction and generate more free radicals;

example 3

1. A square container with the side length of 80cm and the height of 80cm, and the periphery of the square container is composed of a metal or plastic box body; an air filter, a dryer, an air compressor, a high-voltage electrode discharge ozonizer, a delivery pump and the like are sequentially arranged at the bottom of the box body of the square container; the devices are connected by pipelines, the gas outlet pipeline of the high-voltage electrode discharge ozonizer is connected with a delivery pump by a three-way valve, and the other end of the three-way valve is provided with a valve; the whole system is powered by a power supply system, the power supply adopts commercial power, a PLC (programmable logic controller) is connected beside the power supply, the whole system is regulated and controlled, a frequency converter and a step-up transformer are connected beside the power supply to change the frequency and the voltage of alternating current and control the ozone output speed, ozone is output by a delivery pump and enters a circular or square water tank with the volume of 5 liters to be mixed with water, the ozone dissolved in the water can quickly kill microorganisms and enzyme activity on an article to be clarified, organic substance dirt and toxic organic substances attached to the article are oxidized and decomposed into carbon dioxide and water, nucleic acid is thoroughly decomposed, and the dirt on the article is transferred into an aqueous solution to be oxidized, decomposed and removed by bubbling and oscillation;

3. in the above 2 processes, can be through adjusting the programmable controller in the control panel and realizing automatic control, change the alternating current frequency through the converter, rise from 50Hz to 1200Hz, improve the yield of ozone, change alternating voltage through the transformer, rise voltage from 220V to 15000V, improve high-voltage discharge frequency and efficiency, improve ozone yield and effect. After the whole process, the water discharged from the 2 last step contains active oxygen such as ozone, hydroxyl free radical and the like, the water is put into an object to be disinfected and cleaned in advance, then the ozone and the free radical conveyed into the water by the conveying pump can quickly kill microorganisms on the object to be disinfected and sterilized in a laboratory, biological test materials and molecular biological test materials are inactivated, enzyme activity is killed, DNA and RNA in the biological test materials are decomposed, toxic and harmful small molecular organic compounds on the object (a culture dish, a test tube, a triangular flask, a centrifuge tube, biological tissues, residual microbial preparations for tests, plant and animal test materials and the like) are thoroughly oxidized into water and carbon dioxide, organic poisons such as organic pesticides, antibiotics and the like attached to the wall of the object are thoroughly decomposed into water and carbon dioxide, in addition, the ozone gas continuously bubbles into a water tank and continuously vibrates to accelerate dirt attached to the object to separate into the water solution, continuously inflating, disinfecting, sterilizing and cleaning for more than 30 minutes to achieve good disinfection effect and cleaning effect, breaking DNA in the residual body of the watermelon plant leaves, ensuring that the enzyme activities of protease, DNA enzyme, catalase, peroxidase, superoxide dismutase and the like are almost zero, ensuring that the total number of bacteria is less than 5/g and the total number of fungi is less than 2/g, closing a power supply, pouring a water tank solution, and taking out disinfected and cleaned objects; in addition, the equipment can directly carry out air sterilization through the valve 12, such as air sterilization, aseptic room sterilization, super clean bench sterilization and the like;

4. in the original components of the equipment, the air filter adopts rock wool, a filter screen, a cloth bag and other modes for air filtration; adding active carbon, anhydrous calcium sulfate, anhydrous copper sulfate, etc. into the dryer for dehydration and drying; the distance between the electrodes of the high-voltage electrode discharge ozonizer is 0.5mm, so that the ozone generating effect is good; in order to improve the bactericidal effect and the oxidizing power of the ozone water, 1 g of ferrous sulfate can be added into the water to promote the Fenton reaction and generate more free radicals;

application examples of the invention:

the technology of the invention is applied to the application of laboratory disinfection and cleaning.

1. Referring to example 1, the residual protease, dnase, catalase, peroxidase, superoxide dismutase, etc. in the watermelon test material residue were greatly reduced by 98%, 99%, 99.5%, 96.5%, 98.7% after the treatment, compared with the control without the treatment; the total number of bacteria is reduced by 99.99%, and the total number of fungi is reduced by 98.99% (figure 1);

2. referring to example 2, the residual protease, dnase, catalase, peroxidase, superoxide dismutase and the like in the watermelon test material residue after the treatment by the technique are greatly reduced by 97.9%, 99%, 99.3%, 98.5% and 99.7% respectively compared with the control without the technique; the total number of bacteria is reduced by 99.99 percent, and the total number of fungi is reduced by 99.99 percent;

3. referring to example 3, when the watermelon test material is treated by the technology, compared with a control without the technology, the residual protease, DNA enzyme, catalase, peroxidase, superoxide dismutase and the like in the watermelon test material residue are greatly reduced by 97.3%, 98.7%, 99.2%, 99.5% and 99.2% respectively; the total number of bacteria is reduced by 99.99%, and the total number of fungi is reduced by 98.27%.

Claims

1. The manufacture and application of the laboratory multifunctional disinfection cleaning machine are characterized by comprising the following steps:

a. a square container with the side length of 45-100cm and the height of 50-150cm, and the periphery of the container is composed of a metal or plastic box body; an air filter, a dryer, an air compressor, a high-voltage electrode discharge ozonizer, a delivery pump and the like are sequentially arranged at the bottom of the box body of the square container; the devices are connected by pipelines, the gas outlet pipeline of the high-voltage electrode discharge ozonizer is connected with a delivery pump by a three-way valve, and the other end of the three-way valve is provided with a valve; the whole system is powered by a power supply system, the power supply adopts commercial power, a PLC programmable controller is connected beside the power supply, the whole system is regulated and controlled, a frequency converter and a step-up transformer are connected beside the power supply to change the frequency and the voltage of alternating current and control the ozone output speed, ozone is output by a delivery pump and enters a circular or square water tank with the volume of 5-150 liters to be mixed with water, the ozone dissolved in the water can quickly kill microorganisms and enzyme activity on an article to be cleaned, organic substance dirt and toxic organic substance attached to the article are oxidized and decomposed into carbon dioxide and water, nucleic acid is thoroughly decomposed, and the dirt on the article is transferred into an aqueous solution to be oxidized and decomposed for removal through bubbling and oscillation;

b. after the unit devices in the step a are assembled, a power supply is switched on, an air compressor is started, particulate matter impurities in air are filtered by the air through a filter, the air enters a dryer, is dried by the dryer to remove moisture, enters the air compressor to generate high-pressure compressed air flow, enters a high-pressure electrode discharge ozonizer (consisting of 5-20 pairs of blocking dielectric electrodes), generates high-concentration ozone, is connected with a delivery pump through a three-way valve, starts the delivery pump to deliver the ozone into water in a water tank, is uniformly mixed in the water through bubbling oscillation, simultaneously oscillates and drops dirt and toxic substances on an object to be disinfected to carry out water treatment, generates Fenton reaction and free radical reaction, generates hydroxyl free radicals and the like, and increases the oxidizing power and the sterilizing power;

c. in the b treatment process, automatic control can be realized by adjusting a programmable controller in a control panel, the frequency of alternating current is changed by a frequency converter and is increased from 50Hz to 1200Hz, the yield of ozone is improved, the alternating voltage is changed by a transformer, the voltage is increased from 220V to 15000V, the high-voltage discharge frequency and efficiency are improved, and the yield and the effect of ozone are improved. After the whole process, the last effluent of the step b contains active oxygen such as ozone, hydroxyl free radical and the like, the articles to be disinfected and cleaned are put into water in advance, then the ozone and the free radicals conveyed into the water by the conveying pump can quickly kill microorganisms on the articles to be disinfected and sterilized in a laboratory, biological test materials and molecular biological test materials are inactivated, enzyme activity is killed, DNA and RNA in the biological test materials are decomposed, toxic and harmful small molecular organic compounds on the articles (such as a culture dish, a test tube, a triangular flask, a centrifuge tube, biological tissues, test residual microbial preparations, plant and animal test materials and the like) are thoroughly oxidized into water and carbon dioxide, organic poisons such as organic pesticides, antibiotics and the like attached to the wall of the article are thoroughly decomposed into water and carbon dioxide, in addition, the ozone gas continuously bubbles into a water tank and continuously vibrates to accelerate dirt attached to the article to be separated into the water solution, continuously inflating, disinfecting, sterilizing and cleaning for more than 30 minutes to achieve good disinfection effect and cleaning effect, turning off a power supply, pouring a water tank solution, and taking out the disinfected and cleaned objects; in addition, the equipment can directly carry out air sterilization through the valve 12, such as air sterilization, aseptic room sterilization, super clean bench sterilization and the like;

d. in the original components of the equipment, the air filter adopts rock wool, a filter screen, a cloth bag and other modes for air filtration; adding active carbon, anhydrous calcium sulfate, anhydrous copper sulfate, etc. into the dryer for dehydration and drying; the distance between the electrodes of the high-voltage electrode discharge ozonizer is 0.5-4mm, so that the ozone generating effect is good; in order to improve the sterilization effect and the oxidizing power of the ozone water, 0.1-1 g of ferrous sulfate can be added into the water tank to promote the Fenton reaction and generate more free radicals.

2. The manufacturing and application of the laboratory multifunctional disinfection cleaning machine as claimed in claim 1, characterized in that the method is a square container with 45-100cm side length and 50-150cm height, and the periphery is composed of a metal or plastic box body; an air filter, a dryer, an air compressor, a high-voltage electrode discharge ozonizer, a delivery pump and the like are sequentially arranged at the bottom of the box body of the square container; the devices are connected by pipelines, the gas outlet pipeline of the high-voltage electrode discharge ozonizer is connected with a delivery pump by a three-way valve, and the other end of the three-way valve is provided with a valve; the whole system is powered by a power supply system, the power supply adopts commercial power, a PLC programmable controller is connected beside the power supply, the whole system is regulated and controlled, a frequency converter and a step-up transformer are connected beside the power supply to change the frequency and the voltage of alternating current and control the ozone output speed, ozone is output by a delivery pump and enters a circular or square water tank with the volume of 5-150 liters to be mixed with water, the ozone dissolved in the water can quickly kill microorganisms and enzyme activity on an object to be clarified, organic substance dirt and toxic organic substances attached to the object are oxidized and decomposed into carbon dioxide and water, nucleic acid is thoroughly decomposed, and the dirt on the object is transferred into an aqueous solution to be oxidized and decomposed for removal through bubbling oscillation.

3. The manufacturing and application of the multifunctional laboratory disinfection and cleaning machine according to claim 1, wherein in the method, after the unit devices in the step a are assembled, a power supply is switched on, an air compressor is started, the air is filtered by a filter to remove particulate matters and impurities in the air, the air enters a dryer, is dried by the dryer to remove moisture, enters the air compressor to generate high-pressure compressed air flow, enters a high-pressure electrode discharge ozone device (consisting of 5-20 pairs of barrier dielectric electrodes) to generate high-concentration ozone, is connected with a delivery pump through a three-way valve, the delivery pump is started to deliver ozone into the water in the water tank, and the ozone is bubbled and vibrated, mixing in water, shaking off dirt and toxic matter on the article to be disinfected, and generates Fenton reaction and free radical reaction to generate hydroxyl free radical and the like, thereby increasing the oxidizing power and the bactericidal power.

4. The manufacturing and application of the laboratory multifunctional disinfection and cleaning machine according to claim 1, characterized in that said method, in the above b treatment process, can realize automatic control by adjusting the programmable controller in the control panel, change the alternating current frequency by the frequency converter, raise the ozone yield from 50Hz to 1200Hz, change the alternating current voltage by the transformer, raise the voltage from 220V to 15000V, raise the high voltage discharge frequency and efficiency, improve the ozone yield and effect. After the whole process, the last effluent of the step b contains active oxygen such as ozone, hydroxyl free radical and the like, the articles to be disinfected and cleaned are put into water in advance, then the ozone and the free radicals conveyed into the water by the conveying pump can quickly kill microorganisms on the articles to be disinfected and sterilized in a laboratory, biological test materials and molecular biological test materials are inactivated, enzyme activity is killed, DNA and RNA in the biological test materials are decomposed, toxic and harmful small molecular organic compounds on the articles (such as a culture dish, a test tube, a triangular flask, a centrifuge tube, biological tissues, test residual microbial preparations, plant and animal test materials and the like) are thoroughly oxidized into water and carbon dioxide, organic poisons such as organic pesticides, antibiotics and the like attached to the wall of the article are thoroughly decomposed into water and carbon dioxide, in addition, the ozone gas continuously bubbles into a water tank and continuously vibrates to accelerate dirt attached to the article to be separated into the water solution, continuously inflating, disinfecting, sterilizing and cleaning for more than 30 minutes to achieve good disinfection effect and cleaning effect, turning off a power supply, pouring a water tank solution, and taking out the disinfected and cleaned objects; in addition, the apparatus can be sterilized directly by air through the valve 12, and air sterilization in a sterile room, a clean bench, or the like can be performed.

5. The manufacturing and application of the laboratory multifunctional disinfection and cleaning machine as claimed in claim 1, wherein in the method, in the original equipment composition, the air filter adopts rock wool, filter screen, cloth bag and other modes for air filtration; adding active carbon, anhydrous calcium sulfate, anhydrous copper sulfate, etc. into the dryer for dehydration and drying; the distance between the electrodes of the high-voltage electrode discharge ozonizer is 0.5-4mm, so that the ozone generating effect is good; in order to improve the sterilization effect and the oxidizing power of the ozone water, 0.1-1 g of ferrous sulfate can be added into the water tank to promote the Fenton reaction and generate more free radicals.

6. Development of the laboratory multifunctional disinfection and cleaning machine according to any one of claims 1 to 5 and application thereof in disinfection and cleaning of articles in microbiological laboratories, biological laboratories, botany laboratories, zoology laboratories, molecular biology laboratories, transgenic laboratories, physiological and biochemical laboratories and disinfection of laboratory air.