KR100825978B1 - Skin protecting compositions - Google Patents

Abstract

Invasion of the mucous membranes of the eyes, nose, throat, ears, anus, and genital area, the wound and the skin into the body of infectious bacteria such as Pseudomonas aeruginosa, Bacillus subtilis, Anthrax, Escherichia coli, Staphylococcus aureus, MRSA, and viruses Skin protective compositions capable of effectively inhibiting and inhibiting their proliferation; Skin protection compositions which can effectively prevent in-hospital infections by resistant bacteria; Compositions for the prophylaxis and / or treatment of bacterial infections or viral infections including intravenous infections; antiseptic; Provided is a filtration device that can effectively suppress the invasion of an infectious bacterium or virus and at the same time inhibit its proliferation. Skin protection composition, comprising skin care extract, or skin care composition which can effectively prevent in-hospital infection caused by resistant bacteria; Compositions for the prophylaxis and / or treatment of bacterial infections or viral infections including intravenous infections; antiseptic; Or a filtration device that can effectively suppress the invasion of infectious bacteria or viruses.

Skin protection composition, bacterial infection, viral infection, filtration device, stalk extract

Description

Skin protection composition {SKIN PROTECTING COMPOSITIONS}

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to skin protection compositions, and in particular, effectively inhibits the invasion of infectious bacteria and viruses from the mucous membranes of the eyes, nose, throat, ears, anus, genitals, and the like into the body, and simultaneously inhibits their proliferation. It relates to a skin care composition that can be. The present invention also relates to preservatives. The present invention also relates to a filtration apparatus that can effectively prevent the invasion of infectious bacteria and viruses and at the same time suppress the proliferation thereof.

Infectious bacteria such as Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli, Staphylococcus aureus and viruses such as influenza virus are suspended in the air and invade the body from the mucous membranes or wounds of humans or animals, causing harmful infections in humans and animals. . Various antibiotics have been used for these bacterial infections. However, as a result of the abuse of antibiotics, various antibiotic-resistant bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), which do not contain any conventional antibiotics, have emerged. As a result, in hospitals, patients, doctors, nurses, and the like are infected with resistant bacteria, and in some cases deaths are caused.

Meanwhile, mail containing anthrax in recent years has been delivered to politicians, mass media officials, and the like, and many unopened parties have been infected with anthrax and deaths have caused social fear. There is an urgent need for appropriate defenses against biological weapons, not only among those engaged in public affairs, but also among ordinary citizens.

Accordingly, an object of the present invention is to provide Pseudomonas aeruginosa, Bacillus subtilis, Anthrax, Escherichia coli, Staphylococcus aureus, MRSA, Gas necrosis, Tetanus, Botulinus from the mucous membranes of eyes, nose, throat, ears, anus, genitals, etc. The present invention provides a skin protection composition capable of effectively inhibiting invasion of infectious bacteria such as bacteria, viruses such as herpes virus, influenza virus, corona virus, and the like, and inhibiting its proliferation.

It is another object of the present invention to provide a skin protection composition which can effectively prevent internal infection and the like caused by so-called resistant bacteria.

It is another object of the present invention to provide a composition for the prevention and / or treatment of bacterial infections or viral infections including intravenous infection.

Another object of the present invention is to provide a preservative.

Still another object of the present invention is to provide a filtration apparatus that can effectively suppress the invasion of an infectious bacterium or virus and at the same time suppress the proliferation thereof.

The present invention provides a skin protection composition, a composition for preventing and / or treating a bacterial infection or a viral infection, a preservative and a filtering device.

1.Skin protection composition containing the stalk extract as an active ingredient.

2. A composition for the prevention and / or treatment of bacterial infections containing the extract of stalks as an active ingredient.

3. A composition for the prevention and / or treatment of a viral infection, which comprises as an active ingredient a stalk extract.

4. A composition for the prevention and / or treatment of intestinal infections containing the extract of Zip as an active ingredient.

5. The composition according to any one of 1 to 4, further containing an organic acid.

6. The composition according to any one of 1 to 5 above, which is in the form of an oral composition.

7. Gummi, jelly, troche, candy, chewing gum, jam, sherbet, cream, candy, castella, cookies, chocolate, senbei snacks such as chocolate, bread, noodles, drinks, tablets, pills, mouthwashes, ferns The composition according to the above 6, which is in the form of an agent, a toothpaste, a skin patch film, and a spray for treating pharyngeal inflammation.

8. The composition according to any one of 1 to 5 above in the form of a protective cloth.

9. Gauze, mask, eye patch, sanitary napkin, sanitary napkin, bandage, toilet paper, hemorrhoid treatment gauze, toilet seat, shoe insole, earmuffs, gloves, hat, white, sheet, cushion cover, pillow cover, curtain, wallpaper, carpet The composition according to the above 8 in the form of a surgical suture.

10. The composition according to the above 8 in the form of a gauze.

11. A mask comprising the gauze according to 10 above.

12. Preservatives containing scavenger extract as an active ingredient.

13. The antiseptic according to 12, wherein the active ingredient further contains an organic acid.

14. Filtration device containing scavenger extract as an active ingredient.

15. The filtration device according to 14 above, wherein the active ingredient further contains an organic acid.

16. The filtration device according to the above 14 or 15, which is in the form of a filter used for a portion of the air passing through the ventilation fan, the air conditioner, the air intake port, the air outlet port, the wire mesh door, the air purifier, and the electric vacuum cleaner.

17. An air purifier comprising the filtration device according to any one of 14 to 16 above.

Hereinafter, the present invention will be described in detail.

Stalk extract

The stalk which is used as a raw material of the stalk extract which is the active ingredient of the present invention is not particularly limited, and all stalks belonging to Sasa are exemplified. For example, the gold pan, the stain pan, Okuyama pan, Ezomiyama pan, Jeju pan, Yahiko pan, Oba pan, Miyama pan, Sendai pan, Yukawa pan, Aboy pan, Onuka pan and so on. Among these, extracts, such as a gold scoop and a stain sack (Chinese sack), are mentioned as a specific example of a commercial item. For example, a water extract of a gold band stalk, a stain stalk, etc. which were collected in July-October from Teshi-san, Hokkaido is preferable.

The stalk extract for use in the present invention is preferably obtained by extracting the leaves or dried leaves of the stalks at atmospheric pressure or under pressure in water at 100 to 180 ° C.

Although the extraction method is not specifically limited, For example, the method of Unexamined-Japanese-Patent No. 3212278 (Unexamined-Japanese-Patent No. 11-196818) can be used. More specifically, the extract is extracted by treatment at 100 to 180 ° C. for 5 to 30 minutes using a pressurized hot water extractor, and the extract is separated from the hydrous solids (water content of 40 to 70%) by a water separator, followed by saturated steam heating. After 5 ~ 60 minutes of this water-containing solid content at 100 ~ 200 ℃ by the treatment machine, it is once again processed by pressurized hot water extractor at 100 ~ 180 ℃ for 5 ~ 30 minutes to extract the extract and use the first and second extracts together do. In addition, extracts obtained by extracting the dried leaves of the stalks, for example, for about 30 minutes to 12 hours in water at 60 to 100 ° C. can also be used.

Commercially available products containing 50% by mass of the extract of the stalks include Chloroland Molyse Co., Ltd. (AHSS).

The soda extract thus obtained contains a sulfur component, and the content thereof is about 4 to 10 mg, usually about 6 to 9 mg, per 1 g of the solid content of the soda extract. The main component of the sulfur component is considered to be sulfur-containing amino acid.

The composition of the present invention preferably contains 4 to 500 mg, more preferably 8 to 250 mg, most preferably 16 to 150 mg of sulfur component derived from the scavenger extract per 100 g in terms of sulfur.

In addition, the sasa extract contains tannin and its content is about 5 to 15% by mass based on the solid content of the sasa extract.

It is preferable that the composition of this invention contains stalk tannin in solid content concentration preferably 0.05-7.5 mass%, More preferably, 0.1-6 mass%.

The composition of the present invention may comprise only the extract of the stalk as an active ingredient, and by using an appropriate amount of an organic acid in combination therewith, the therapeutic and / or prophylactic effect can be further improved. Examples of such organic acids include malic acid, citric acid, lactic acid, oxalic acid, maronic acid, succinic acid, fumaric acid, acetic acid, benzoic acid, phenyl acetic acid, salicylic acid, and phenols.

The amount of the organic acid used is preferably 0.01 to 5% by mass, more preferably 0.02 to 3% by mass, and most preferably 0.05 to 1.5% by mass in the composition of the present invention.

Skin protection compositions, compositions for preventing and / or treating bacterial and viral infections

Skin protection compositions of the present invention, bacterial infections and viral infection prevention and / or composition for the treatment of mucous membranes such as eyes, nose, throat, ears, anus, genitals, wound site and skin invading infectious bacteria or viruses into the body. It effectively inhibits and effectively prevents and / or treats bacterial infections including intravenous infections and viral infections such as influenza virus, herpes virus and corona virus.

The present invention may be a skin protective cloth or oral composition in the form of a skin protective composition containing a scavenger extract as an active ingredient, a composition for the prevention and / or treatment of bacterial or viral infections.

Skin protective cloths are breathable carriers such as natural fibers such as silk, cotton, hemp, wool, synthetic fibers such as polyurethane, vinylon, polypropylene, nylon, polyester, acrylic, semisynthetic fibers, or two of them. The fiber extract or the yarn of the above mixture or the woven fabric, knitted fabric, nonwoven fabric, paper or the like is contained by a method such as impregnation or spraying. In this specification, 'protective fabric' is intended to include not only the fabric, but also the fiber itself, thread, paper, and the like.

Specific forms of protection include direct contact with mucous membranes or skin classified as hygiene products such as gauze, masks, eye masks, sanitary napkins, sanitary napkins, bandages, toilet paper, hemorrhoids gauze, toilet seats, shoe insoles, earplugs and band-aids. In addition to fabrics, clothing accessories such as clothing, gloves, hats, socks, sheets, baltosies, upholstery and interior materials such as bedding, curtains, wallpaper, carpets, beddings such as sheets, cushion covers, pillow covers, and bedding products, Cooking materials, such as articles or cutting boards which directly or indirectly contact skin, such as medical materials, such as a surgical suture room, are mentioned.

Oral compositions containing the stalk extract of the present invention include, for example, gummi, jelly, troche, candy, chewing gum, jam, sherbet, cream, candy, castella, cookies, chocolates, confectionary such as whole cakes, Breads, noodles, beverages, tablets, pills, mouthwashes, dentifrices, toothpastes, skin patch films, spray agents for treating sore throat inflammation, and the like.

In order to contain the stalk extract in the skin protective cloth, for example, the protective cloth may be impregnated in a 2-20 mass% aqueous solution of the stalk extract or sprayed and dried by spraying a 2-20 mass% aqueous solution of the stalk extract on the protective cloth. At this time, the impregnation amount or spraying amount of the stalk extract is preferably 2 to 20% by mass, more preferably 6 to 15% by mass, and most preferably 8 to 12% by mass as the solid content of the stalk extract. If it is less than 2 mass%, the expression of the target effect may be inadequate, and if it exceeds 20 mass%, skin or mucous membranes may tingle, and the effect does not improve so uneconomically.

The skin protection composition of the present invention is preferably 8 to 200 mg, more preferably 24 to 150 mg, most preferably 32 to 120 mg per 100 g of the protective composition in terms of sulfur in terms of sulfur derived from the extract of the stalk It contains.

For example, Gummi extract, gummi, jelly, troche, candy, chewing gum, jam, sherbet, cream, candy, castella, cookies, chocolate, rice cakes, breads, noodles, beverages, tablets, pills ( For example, in order to be contained in oral compositions such as scavenger), mouthwash, dentifrice, toothpaste, and skin patch film, the solid content of the scavenger extract with respect to the oral composition raw material is preferably 2 It is suitable to add -20 mass%, More preferably, 6-15 mass%, Most preferably, about 8-12 mass%. If it is less than 2 mass%, the expression of the target effect may be inadequate, and when it exceeds 20 mass%, skin and mucous membranes may tingle, and since an effect does not improve so much, it is uneconomical.

When containing or after containing the sachet extract in the protective cloth or oral composition, the heat treatment is performed at a temperature of 70 to 90 ° C., preferably 75 to 85 ° C. for 30 minutes to 3 hours, preferably 1 to 2 hours. desirable. The heat treatment significantly improves the skin protection effect.

The oral composition of this invention can be mix | blended a conventional component suitably according to a dosage form. Examples include excipients such as glucose, lactose, sucrose, starch syrup, dextrin and cyclodextrin, binders such as gum arabic, sodium carboxymethylcellulose, crystalline cellulose and gumbase, disintegrating agents such as starch, magnesium stearate and sucrose fatty acid esters. Lubricants such as lubricants, fragrances, chlorophyll, peppermint, menthol, and the like.

When the skin protection composition of the present invention is used in the form of a protective cloth, a sachet extract is contained in a protective cloth (gauze or the like) in contact with a mucous membrane or a wound site, and this is to be replaced once or three times a day. good. When used in the form of a surgical suture chamber, the invasion of bacteria from the suture wound is effectively suppressed to suppress inflammation of the wound and promote recovery of the surgical site.

When the skin protection composition of the present invention is used in the form of a protective cloth for prevention of in-hospital infection in a hospital or the like, the infection prevention effect of the skin protection composition of the present invention lasts for a long time. When the effect is reduced by washing, the treatment may be appropriately exchanged or a treatment for containing the scavenger extract again.

In the case of using the skin protection composition of the present invention in the form of an oral composition, the intake is not particularly limited, but the solid content of the scavenger extract is about 2 to 15 mg, for example, 1 to 5 times a day, usually 1 to 3 Eat about times. The amount of intake and the number of intakes may be appropriately increased or decreased depending on the purpose.

The skin protection composition of the present invention contains 2-20% by mass of the stalk extract as a solid, and shows a remarkable bactericidal effect even against resistant bacteria to which antibiotics are not known.

In addition, when the skin protection composition of the present invention is used as an oral composition, by appropriately staying in the mouth when necessary, it is very easy to prevent the infection of infectious bacteria or viruses and to suppress the proliferation thereof. In addition, compositions having a slowly released form such as chewing gum and candy can exert their effects over a long period of time, which is advantageous.

Skin protection compositions of the present invention can be used in the form of ointments, creams, gels, gels, lotions, oils, soaps, shampoos and the like. Ointments, creams, gels, gels, lotions or oils (for example, jojoba oil, grapemede oil and rice germ oil) containing 2 to 20% by weight solids extract can be easily infectious. It can prevent the infection of bacteria and viruses and suppress its proliferation.

antiseptic

The present invention also provides a preservative containing the scavenger extract as an active ingredient. The form at the time of using as a preservative is not specifically limited. Examples of the composition to be preserved include food, beverages, seasonings, cosmetics (including lotions and oils), sprays for treating wound sites, sprays for treating sore throat inflammation, and the like. It is suitable to contain the sachet extract in these objects as solid content preferably 2-20 mass%, More preferably, 6-15 mass%, Most preferably, about 8-12 mass%. If it is less than 2 mass%, the expression of the desired antiseptic effect may be insufficient, and if it exceeds 20 mass%, it is uneconomical because there is not much improvement of the effect, and when it is added to food, the taste of the food changes. It is not desirable to have.

Filtration device

The present invention also provides a filtration device of air containing the scavenger extract as an active ingredient. Specific examples thereof include a filter used in a portion through which air passes, such as a ventilation fan, an air conditioner, an air intake port, an air outlet port, a wire mesh door, an air purifier, and an electric vacuum cleaner. The material of the filter is not particularly limited, but natural fibers such as silk, cotton, hemp, wool, synthetic fibers such as polyurethane, polypropylene, nylon, polyester, acrylic, vinylon, semisynthetic fiber, glass fiber or two of them The above mixture woven fabric, knitted fabric, nonwoven fabric, paper, etc. are mentioned. What is necessary is just to contain the water dilution liquid of a sachet extract in these filters by methods, such as impregnation and spraying, and to dry. The content of the scavenger extract in the filter is preferably 2 to 20% by mass, more preferably 4 to 15% by mass, and most preferably about 6 to 8% by mass as a solid content. If it is less than 2 mass%, the expression of the desired bactericidal, bactericidal, and propagation preventing effect may be insufficient, and even if it exceeds 20 mass%, the effect is not much improved, which is uneconomical. Passing through this filter prevents the growth of bacteria and viruses on the filter because bacteria and viruses present in the air are filtered and sterilized or their growth is inhibited.

The present invention provides a skin protection composition, an antiseptic agent, and a filtering device, in particular, effectively inhibits the invasion of infectious bacteria or viruses from the mucous membranes of the eyes, nose, throat, ears, anus, genitals, and the wound into the body. Proliferation can be suppressed.

Hereinafter, although a reference example, an Example, and a test example are shown and this invention is demonstrated in more detail, this invention is not limited to these. In addition, unless otherwise indicated in this specification, "%" is the "mass%."

Reference Example  1: Preparation of Garlic Extract

The dried leaves of stained stalks collected in September in Teshiosan, Hokkaido were placed in a pressurized hot water extraction tank and treated at 125 ° C. for 10 minutes, and the hot water was cooled to about 80 ° C. in cooling water, and then the extract and the hydrous solid were screw-pressed. The water content was set at about 50% by mass by separating from. Next, about 50 mass% aqueous solids were put into an autoclave, and pressurized heat processing by saturated steam was performed for 10 minutes at 180 degreeC. The treated hydrous solid was once again placed in a pressurized hot water extraction tank and treated at 110 ° C. for 5 minutes to extract the extract. The first and second extracts were combined, filtered through diatomaceous earth, concentrated under reduced pressure to 50% by mass of solids, and subjected to flow sterilization at 110 to 130 ° C. to obtain a scavenger extract.

The sulfur content in this sachet extract was 3850 µg / ml (7.7 mg / solid content 1 g).

Reference Example  2: organic acid-containing scavenger extract

0.5 g, 1 g, 1.5 g or 2 g of malic acid was added to 100 g of the stalk extract (50 mass% of solid content) prepared in Reference Example 1 to prepare an organic acid-containing stalk extract.

Example  One : Chewing gum  Produce

To 100 g of the gum base heated to 60 ° C. in a thermostatic chamber for 30 minutes, 2 to 15 g of the stalk extract (solid content of 50% by mass) prepared in Reference Example 1 was added, opened for 2 minutes, and further heated at 60 ° C. for 10 minutes. The obtained dough was allowed to cool and rolled to produce a plate-shaped chewing gum (and chewing gum of various forms such as annular shapes) having a thickness of about 1 mm.

Example  2 : Pilled  Produce

The compound of the following composition (unit: mass%) was mixed uniformly with the kneader, and then these were shape | molded by the pill maker, and the pill was manufactured.

Scavenger extract of the reference example 1 (50 mass% of solid content) 20

Subline 10

Licorice 15

Cinnamon 15

Fennel 5

Mint 5

Time 5

Ginger 5

Cloves 5

Reduction 5

Cornstarch 8

Magnesium oxide 2

  Total 100

Example  3: protective gauze

Spray gauze extract prepared in Reference Example 1 (50 mass% of solid content) diluted with water 10 times with silk gauze, and sprayed at 80 ° C. for 1 hour, and a protective gauze containing 8.8 mass% of the scavenger extract as a solid content. Prepared.

Example  4: protective gauze

Silk gauze was immersed for 1 hour at 80 ° C. in a solution diluted 6 times with water (50 mass% of solid content) prepared in Reference Example 2 at 80 ° C., and dried at 80 ° C. for 1 hour. A protective gauze containing% was prepared.

Example  5: protective mask

The protective gauze of Example 3 or 4 was inserted inside the mask which consists of several sheets of gauze, and the protective mask was manufactured.

Example  6: air Purifier

A filter used in a conventional air purifier was immersed at 80 ° C. for 1 hour in a solution diluted 6 times with water (50 mass% of solid content) prepared in Reference Example 2 at 80 ° C., and dried at 80 ° C. for 1 hour. The filter containing 8 mass% as solid content was manufactured. It was mounted on an air purifier.

Test Example  1: antimicrobial test

The protective gauze prepared in Example 3 (containing 8.8 mass% of scavenger extract solids) and silk gauze (control gauze) containing no scavenger extract were prepared.

To each gauze (5 × 7 cm), 1 ml of the following bacterial solution (about 5.0 × 10 ⁴ cells / ml or about 5.0 × 10 ⁵ cells / ml) was added dropwise, followed by standing incubation at 37 ° C. for 12 hours in an incubation period. After the incubation, the gauze was sufficiently washed with 10 ml of liquid medium to prepare a test liquid. This was applied to a Brainheart agar plate medium using a spiral system (NASA system, USA). It was left to incubate for 18 hours at 37 ° C. in the incubator. The number of colonies on the Brainheart agar plate was measured. The results are shown in Table 1 and Table 2.

Germ Inoculation Count Number of bacteria after preservation Gauze of the present invention Contrast gauze Staphylococcus aureus 5.0 × 10 ⁴ 2.0 × 10 (<10 ² ) > 10 ⁶ MRSA 5.1 × 10 ⁴ 1.0 × 10 (<10 ² ) > 10 ⁶ Pseudomonas aeruginosa 5.6 × 10 ⁴ 5.0 × 10 (<10 ² ) > 10 ⁶ Escherichia coli 5.2 × 10 ⁴ 8.0 × 10 (<10 ² ) > 10 ⁶ Bacillus subtilis 5.4 × 10 ⁴ 9.0 × 10 (<10 ² ) > 10 ⁶

Germ Inoculation Count Number of bacteria after preservation Gauze of the present invention Contrast gauze Staphylococcus aureus 5.4 × 10 ⁵ 5.0 × 10 (<10 ² ) > 10 ⁷ MRSA 5.5 × 10 ⁵ 6.0 × 10 (<10 ² ) > 10 ⁷ Pseudomonas aeruginosa 5.6 × 10 ⁵ 9.0 × 10 (<10 ² ) > 10 ⁷ Escherichia coli 5.2 × 10 ⁵ 9.8 × 10 ² ) > 10 ⁷ Bacillus subtilis 5.4 × 10 ⁵ 8.2 × 10 ² ) > 10 ⁷

In Table 1 and Table 2, the numbers in parentheses indicate the number of bacteria by a general display.

In the stalk extract-containing gauze of the present invention, after 12 hours of incubation, the number of bacteria decreased to about 1/1000 to 1 / 000,000, whereas in the gauze containing no stalk extract, the number of bacteria increased by about 100 times or more. have. This indicates that the stalk extract-containing gauze of the present invention has excellent antimicrobial properties, and particularly remarkable antimicrobial activity against resistant bacteria MRSA which antibiotics do not listen to.

Test Example  2: growth inhibition test for anthrax

1. Test Method

Strains used: Aposome of anthrax 34-F2 (toxin production, non-capsular apothecary). It was adjusted to 34000 pieces per 0.1 ml.

Test liquid: 50% by mass of the extract of solid extract

Test Method: The test solution was diluted with sterile distilled water, adjusted to 25%, 12.5%, 6.25%, 3.2%, 1.6%, 0.8%, and 0.4% liquid, and the following two tests were performed. Sterile distilled water was used as a control.

1) 0.1 ml of anthrax 34-F2 apolyte was mixed with 1 ml of each of the 10 to 50% test liquids prepared above, which was allowed to stand at 37 ° C. for 24 hours, followed by 0.1 ml of Brain Heart Infusion (BHI). After spreading on agar medium for 24 hours at 37 ℃, colony growth was observed. Thereafter, the increase and decrease of the number of bacteria was also observed with sterile distilled water.

2) Agar plates were prepared by adding BHI agar at twice the concentration to 10 ml of each of the 10 kinds of 50 ~ 0% test solution prepared above. 0.1 ml of anthrax solution of anthrax 34-F2 was smeared on each medium and cultured for 24 hours at 37 ° C, and colony growth was observed.

2. Results

The results are shown in Table 3 below.

Garlic Extract Solid Concentration (%) 25 12.5 6.25 3.2 1.6 0.8 0.4 0.2 0.1 0 - - - - - - +/- + + +

+: Colony development similar to contrast

-: No colony observed

+/- colony formation is recognized but less than contrast

3. Conclusion

Although the sterilization ability against the anthrax apoptose was not confirmed, the tested saponica extract strongly inhibited the growth of anthrax. As a result, the skin protection composition of the present invention containing the scavenger extract can suppress the growth of anthrax and consequently prevent infection by anthrax.

Test Example  3: antiviral effect test for influenza virus

1. Test Method

The virus uses herpes simplex virus (HSV) HF strain, herpes simplex virus (HSV) UW strain, influenza virus (Inf.) A / PR / 8 strain, and influenza virus uses (Inf.) A / FM / 1/47 strain. It was. The cultured cells used were Vero cells (derived from African green monkey kidneys) and MDCK cells (derived from canine kidneys).

For the antiviral effect, 10 μl of the virus solution was mixed with 1 ml of the dipping water prepared in Reference Example 1, diluted with sterile distilled water. The plate was inoculated and cultured in a 37 ° C. CO ₂ incubator, and the degree of cell degeneration effect (CPE) was determined by the following criteria. Distilled water (DW) was used for control.

NT: Not tested, 4+: CPE in front of well, 3+: CPE in at least 75% of wells, 2+: CPE in 50-75% of wells, +: CPE in 25-50% of wells, ±: CPE,-: CPE not observed below 25% of wells

The electron microscopic sample was infected with the virus for 20 hours in the cultured cells, treated with the dilute sap extract, and fixedly observed according to the conventional method.

2. Results

Simple Herpes Virus (HSV) HF strain, Simple Herpes Virus (HSV) UW strain, Influenza Virus (Inf.) A / PR / 8 strain, Influenza Virus (Inf.) Antiviral against A / FM / 1/47 strain The effects are shown in Tables 4 to 7.

HSV HF Note Garlic Extract Solid Concentration (%) 10 8 5 3 2 One Distilled water 1 minute 3+ 4+ 4+ 4+ 4+ 4+ 4+ 5 minutes - ± 4+ 4+ 4+ 4+ 4+ 10 minutes - - - 2+ 4+ 4+ 4+ 15 minutes - - - - ± 2+ 4+ 30 minutes - - - - - - 4+ 60 minutes - - - - - - 4+

Virus was inactivated by treatment for 5 minutes at 10% concentration and inactivated by treatment for 1 hour even at 1% concentration.

HSV UW Garlic Extract Solid Concentration (%) 10 8 5 3 2 One Distilled water 1 minute - ± 2+ 2+ 3+ 4+ 4+ 5 minutes - - 1+ ± 2+ 3+ 4+ 10 minutes - - - - ± ± 4+ 15 minutes - - - - - - 4+ 30 minutes - - - - - - 4+ 60 minutes - - - - - - 4+

Virus was inactivated by treatment for 1 minute at 10% concentration and inactivated by treatment for 15 minutes at 1% concentration.

Inf. A / PR / 8 weeks Garlic Extract Solid Concentration (%) 5 2 One 0.5 0.1 Distilled water 1 minute 4+ 4+ 4+ 4+ 4+ 4+ 5 minutes 4+ 4+ 4+ 4+ 4+ 4+ 10 minutes 2+ 2+ 2+ 2+ 4+ 4+ 15 minutes 2+ 4+ 2+ 2+ 4+ 4+ 30 minutes - - - - 2+ 4+ 60 minutes - - - - 2+ 4+

Virus was inactivated by treatment for at least 0.5% concentration for 30 minutes.

Inf. A / FM / 1/47 weeks Garlic Extract Solid Concentration (%) 5 2 One 0.5 0.1 Distilled water 1 minute 4+ 4+ 4+ 4+ 4+ 4+ 5 minutes 4+ 4+ 4+ 4+ 4+ 4+ 10 minutes 4+ 4+ 2+ + 2+ 4+ 15 minutes 4+ 4+ - + 2+ 4+ 30 minutes 4+ 4+ + - ± 4+ 60 minutes 2+ 2+ - - - 4+

Virus was inactivated by treatment for at least 0.5% concentration for 30 minutes.

3. Conclusion

From these results, it was clear that the antiviral effect of the extract of the scavenger on influenza virus was different from the conventional formulation, but the mechanism of action was unclear.

Claims (8)

In the composition for the prevention and / or treatment of bacterial infections containing the stalk extract as an active ingredient, The bacterium is anthrax, The pans are at least one selected from the group consisting of gold pans, stain pans, Okuyama pans, Ezomiyama pans, Jeju pans, Yahiko pans, Oba pans, Miyama pans, Sendai pans, Yukawa pans, Aboy pans, and Onuka pans. Of The composition, characterized in that containing 2 to 20% by weight of the extract as a solid. The composition according to claim 1, further comprising an organic acid as said active ingredient. The composition of claim 1 in the form of an oral composition. The method of claim 3, characterized in that it is in the form of gummi, jelly, troche, candy, chewing gum, jam, sherbet, cream, candy, castella, cookie, chocolate, senbei, confectionary, bread, noodles or beverage. Composition. The composition of claim 1 in the form of a protective cloth. The method according to claim 5, wherein the gauze, mask, eye patch, sanitary napkin, sanitary napkin, bandage, toilet paper, hemorrhoid treatment gauze, toilet seat, shoe insole, earplugs, gloves, hat, white, sheet, cushion cover, pillow cover, curtain Composition in the form of a wallpaper, a carpet or a surgical suture. 7. A composition according to claim 6 in the form of gauze. A mask comprising the gauze according to claim 7.