WO2020256288A1 - Anti-atopy and anti-vaginitis tissue containing cypress extract and preparation method thereof - Google Patents
Anti-atopy and anti-vaginitis tissue containing cypress extract and preparation method thereof Download PDFInfo
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- WO2020256288A1 WO2020256288A1 PCT/KR2020/006550 KR2020006550W WO2020256288A1 WO 2020256288 A1 WO2020256288 A1 WO 2020256288A1 KR 2020006550 W KR2020006550 W KR 2020006550W WO 2020256288 A1 WO2020256288 A1 WO 2020256288A1
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0208—Tissues; Wipes; Patches
-
- A—HUMAN NECESSITIES
- A45—HAND OR TRAVELLING ARTICLES
- A45D—HAIRDRESSING OR SHAVING EQUIPMENT; EQUIPMENT FOR COSMETICS OR COSMETIC TREATMENTS, e.g. FOR MANICURING OR PEDICURING
- A45D44/00—Other cosmetic or toiletry articles, e.g. for hairdressers' rooms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9755—Gymnosperms [Coniferophyta]
- A61K8/9761—Cupressaceae [Cypress family], e.g. juniper or cypress
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
-
- A—HUMAN NECESSITIES
- A45—HAND OR TRAVELLING ARTICLES
- A45D—HAIRDRESSING OR SHAVING EQUIPMENT; EQUIPMENT FOR COSMETICS OR COSMETIC TREATMENTS, e.g. FOR MANICURING OR PEDICURING
- A45D2200/00—Details not otherwise provided for in A45D
- A45D2200/10—Details of applicators
- A45D2200/1009—Applicators comprising a pad, tissue, sponge, or the like
- A45D2200/1027—Tissues, i.e. thin applicator sheets
Definitions
- the present invention relates to an anti-atopy and anti-vaginitis tissue containing a cypress extract and a method for preparing the same, and more particularly, a cypress extract having anti-atopic and anti-vaginitis effects without separately adding a chemical preservative or chemical emulsifier to the tissue. It relates to a tissue containing as an active ingredient and a method of manufacturing the same.
- epidermal bacteria residing in the skin include Staphylococcus epidermis and Staphylococcus aureus.
- Epidermal Staphylococcus aureus is the most well-known type in the aureus family, and it is commonly present in the skin and mucous membranes of humans and other warm-blooded animals, and is generally not pathogenic, but there is a risk of infection appearing in people with weakened resistance.
- Staphylococcus aureus is an important pathogen for wound infections, boils, and skin infections, and is one of the most common causes of food poisoning. It is also found in 30 to 50% of healthy people. It can also exist in purulent supernatant and saliva, so if personal hygiene is neglected It can be contaminated.
- atopic dermatitis is a chronic and recurrent inflammatory skin disease that begins mainly in infancy or childhood, and is accompanied by itching and dry skin characteristic eczema.
- infancy it starts with eczema on the spread side of the face and limbs, but as it grows, it appears in the form of eczema on the bending part of the arm and the bend behind the knee.
- the skin on the folds is thickened, and the skin is thickened, and eczema on the face is more common than in childhood. The cause of atopy is still unknown.
- a woman's vagina is the most humid and warm place in a woman's body as a passage between the vulva and the uterus. Therefore, since it is an environment suitable for bacteria or fungi, which are the main causes of vaginitis, there is a high possibility of disease. Under normal conditions, many lactobacilli inhabit the vagina and make the vagina weakly acidic to suppress the growth of pathogens or fungi, but women who take antibiotics for a long time, take contraceptives for a long time, use tampons or loops, or are pregnant, childbirth, lactation, menopause, etc.
- vaginitis When the balance of the vagina is broken due to changes in the balance of hormones, vaginitis may occur due to the proliferation of opportunistic pathogens that normally inhibit growth under normal conditions in the vagina.
- vaginitis As vaginitis, bacterial vaginosis, Candida vaginitis, Trichomonas vaginitis, and atrophic vaginitis have been reported. The most common disease is bacterial vaginosis, which is caused by Gardnerella vaginalis. This vaginitis is caused by overgrowth of anaerobic bacteria that existed in less than 1% while the number of lactic acid bacteria in the vagina decreases due to an imbalance in vaginal acidity. Although the causative factor that causes changes in the normal vaginal flora is not known, it is estimated that repeated alkalizing of the vagina with frequent intercourse or vaginal washing plays a role.
- Candida vaginitis a common disease along with bacterial vaginitis, is a disease in which the fungus Candida albicans lives in the moist vagina and causes inflammation.About 75% of women live with the vagina more than once during their lives. It is a common disease in which 45% of women suffer from candidal vaginitis of the vulva and have more than two recurrences per year.
- Korean Patent Publication No. 1997-0025529 describes a disposable wet tissue product containing natural active plant extracts. More specifically, blood circulation, inflammation suppression, astringent effect, irritation suppression, antibacterial effect Thyme, Pot Marigold, Angelica (Thouki), Licorice root, Ginger, Shakuyaku, Lime tree, which have excellent effects on wound healing, metabolism, etc. , Aloe, rosemary, garlic (Galic), oats (Oats) are described a disposable wet tissue product containing one or more naturally active plant extracts.
- Korean Patent Publication No. 2014-0068709 describes a composition of an aroma essential oil and a wet tissue using the same, and more specifically, 0.005 to 0.02% by weight of lavender, 0.001 to 0.01% by weight of rosemary, and 0.001 of ylang-ylang based on the total weight of the composition.
- a composition of an aroma essential oil containing from to 0.01% by weight, from 0.001 to 0.01% by weight of German chamomile, from 0.001 to 0.01% by weight of Roman chamomile, from 0.001 to 0.01% by weight of thyme, and from 0.005 to 0.03% by weight of tea tree, and a wet tissue using the same are described. have.
- Korean Patent Registration No. 10-055045 describes a sanitary wipe including propolis extract, green tea extract, and ethanol, and more specifically, 155,000 cc to 158,000 cc of purified water for sanitary wipes.
- a sanitary wipe including propolis extract, green tea extract, and ethanol, and more specifically, 155,000 cc to 158,000 cc of purified water for sanitary wipes.
- Korean Patent Publication No. 2003-0017845 describes a wet tissue containing germanium and Eoseongcho and a method for manufacturing the same.More specifically, a woven fabric containing herbal herbal ingredients used as skin protection and treatment for skin diseases is described. Or, as a wet tissue containing bamboo salt and grapefruit extract, germanium and Eoseongcho in a pulp fabric, it has excellent skin safety, skin moisturizing effect and sterilization effect, making the skin healthy without any side effects and enhancing skin beauty effects. Wet wipes having a heightening effect are described.
- Korean Patent Registration No. 10-1626575 describes an antibacterial wipe for preventing atopy containing an eco-friendly antimicrobial agent. More specifically, it is prepared by impregnating a wet tissue fabric with a composition for wet tissue, but the composition for wet tissue is purified water; Moisturizer; Surfactants; pH regulator; Essential oil (essential oil) and an antibacterial agent; Including, the antibacterial agent is harmless to the human body, but excellent, including Eoseongcho extract, perilla leaf extract, ginkgo leaf extract, conch extract, cinnamon extract, Japanese pepper extract, sumac root extract and baekduong extract
- Antibacterial wipes comprising an antibacterial composition for wet wipes having antibacterial, deodorant, and atopy prevention effects are disclosed.
- the inventors of the present invention added cypress extract while continuing research to develop tissues that are safe for the human body by containing natural substances and have antibacterial activity against bacteria related to female vaginitis as well as general bacteria and atopic and acne-related bacteria.
- the present invention was completed by finding that a tissue has inhibitory activity against not only general bacteria, but also atopy-related strains and female vaginitis-related strains.
- the technical problem to be solved in the present invention is to provide a tissue having excellent anti-atopy and anti-vaginitis efficacy without containing a chemical synthetic substance.
- Another technical problem to be solved in the present invention is to provide a method for manufacturing the tissue.
- the present invention provides an anti-atopic and anti-vaginitis tissue, characterized in that it contains a cypress extract as an active ingredient.
- the anti-atopy and anti-vaginitis tissue according to the present invention is characterized in that it contains 1 to 40 parts by weight of cypress extract based on 100 parts by weight of the tissue fabric.
- the tissue for anti-atopy and anti-vaginosis comprises at least one extract selected from the group consisting of mugwort extract, moringa extract, bamboo extract, centella extract, pine extract and plantain extract per 100 parts by weight of tissue fabric 1 It characterized in that it contains in an amount of to 20 parts by weight.
- the present invention provides a method for preparing a tissue for anti-atopy and anti-vaginitis, characterized in that the cypress extract is mixed in an amount of 1 to 40 parts by weight based on 100 parts by weight of the tissue fabric.
- the tissue may be a dry tissue or a wet tissue, and it has antibacterial activity, especially anti-atopic and anti-vaginitis effects, and is useful for both general antibacterial, anti-dermatitis, and feminine cleansing. Can be used, especially for infants, women, as well as patients, can be very useful.
- tissue containing cypress extract of the present invention can be very useful in hospitals or nursing homes since barking and itching do not occur even when contacted with the skin for a long time.
- the tissue fabric may be a natural wood or synthetic wood pulp that is commonly used for tissues as wood pulp, and there is no particular limitation thereto.
- vaginitis is Candidal vaginitis
- the causative agent of Candida albicans may be Candida albicans, and also as a bacterial vaginosis, Gardnerella vaginalis, a causative agent of bacterial vaginosis. It can be.
- the tissue containing cypress extract of the present invention exhibits antimicrobial activity against Escherichia coli , Staphylococcus epidermidis , Staphylococcus aureus , and Propionibacterium acnes . It also exhibits excellent antimicrobial against Candida albicans and Gardnerella vaginalis, which are vaginitis causative bacteria.
- Cypress an active ingredient of the present invention, has excellent antibacterial and sterilizing effects against bacteria and is widely used as a material for well-being.
- Cypress wood contains a lot of natural antibacterial substances called phytoncide, so it has excellent sterilization effect and strong water resistance when it comes into contact with water. It is widely used as a cutting board material in recent years because its unique scent spreads deeply to remove odors.
- extract is an extract obtained by extracting each herbal medicine by a conventional method, and includes not only the extract from which the herbal medicine is extracted, but also a dry powder thereof or any form formulated using the same.
- the cypress extract can be obtained by adding 2 to 5 times the amount (w/v) of distilled water and extracting at 90 to 110° C. for 1 to 5 hours and then filtering.
- the cypress can be used for both leaves, flowers and stems, and it is particularly preferable to use leaves, excluding flower peaks.
- the cypress extract can be obtained by adding 3 times the amount (w/v) of distilled water to the leaves, adding 1% (v/v) of salt, extracting at 110° C. for 2 hours, and filtering.
- the extraction method is not limited, for example, cold sediment extraction, ultrasonic extraction, reflux cooling extraction, and the like.
- the cypress extract of the present invention may be all extracts, fractions, purified products, diluted solutions, concentrates, or dried products obtained in each step of extraction, fractionation, or purification (separation, fractionation).
- the anti-atopy and anti-vaginitis tissue is characterized in that it contains 1 to 40 parts by weight, preferably 10 to 20 parts by weight, of cypress extract based on 100 parts by weight of the tissue fabric.
- the cypress extract is used in an amount of less than 1 part by weight, anti-atopic and anti-vaginitis effects cannot be obtained, and when the cypress extract is used in an amount exceeding 40 parts by weight, the cypress flavor is too excessive, which may result in rejection.
- the anti-atopy and anti-vaginitis tissue of the present invention is to further increase the anti-atopy and anti-vaginitis effect, mugwort extract, moringa extract, bamboo extract, centella extract, pine extract and plantain extract It is characterized in that it contains at least one extract selected from the group consisting of 1 to 20 parts by weight based on 100 parts by weight of the tissue fabric.
- the mugwort extract, moringa extract, bamboo extract, centella extract, pine extract, and plantain extract are all preferably added to the tissue fabric, and at this time, mugwort extract, moringa extract, bamboo extract, centella extract, pine extract And plantain extract is preferably added by mixing in a 1:1:1:1:1:1 weight ratio.
- Mugwort which has a long history to appear in the Dangun mythology, contains'cineol', an essential oil that gives off a unique scent, so it suppresses the growth of harmful bacteria in the body and has excellent effects on immunity and detoxification. It is used not only as a food ingredient, but also as a tea, medicinal material, dye, cosmetics, etc.
- the moringa is a legume plant that grows naturally in India and Africa, and is cultivated throughout the tropics and is attracting attention as one of the superfoods because it is rich in nutrients such as antioxidants.
- the bamboo Since the bamboo is a monocotyledonous plant, it does not have rings and does not grow hypertrophy.
- the stem is cylindrical and the center is empty. The surface changes from green to yellow-green, and the bracts fall early.
- the membrane epithelium inside the stem of Wangdae or Somdae is said to be killed, and is used for dentition and hematopoiesis, and the air oil extracted from Wangdae or Somdae is used for high blood pressure and has been known as a panacea.
- bamboo leaves are used as a dentition, acupuncture, and cheongsim.
- Centella asiatica is a parsley family that blooms white or light purple flowers on small fan-shaped leaves covered with serrated edges. The fruit is small oval, with areolae protruding at the tip. Because it grows in warm and humid areas, it is commonly found in the mountains and fields of southern islands such as Jeju Island in Korea. In India, injured tigers have been used as medicine for a long time, calling them tiger grass after seeing them roll over and heal them in places with a lot of centella. Madecassic acid in the leaves and stems of Centella asiatica has been found to have the power to heal inflammation and to heal wounds such as tumors and ulcers, so it is widely used as a raw material for ointments, toothpaste, and cosmetics.
- the pine tree is a coniferous evergreen tree distributed in Korea, Japan, and China. It is always green, and leaves do not resist well. Due to the unique shape of the stem and leaf, it appears in many oriental paintings, and there is also a legend that'a tree waiting for the god to come down from the sky'. There are also various types of pine trees. Three to five leaves are gathered together by Japanese pine, and two leaves are gathered in needle-like form by Chosun brush. Chosunsol is also divided into red pine and heuksong. Heuksong is called Haesong, and it is a pine tree that grows on the seashore and has excellent medicinal properties.
- the plantain is one of the most common weeds that can be seen everywhere, such as in a field or a han-gil.
- One of the plantains is called Chajeoncho, and it is named because the seeds gather in the seat of the wheel where the cart passed, and a drumstick is formed. It is found all over Korea and is also distributed in Japan, Taiwan, China, Siberia, and Malaysia. It is a perennial plant belonging to the plantain family, and its leaves are ovate or oval. In Korea, there are about 10 species in one genus, such as Psyllium Psyllium, Long Leaf Psyllium, Wang Psyllium, and Hair Psyllium.
- the corolla is a funnel-shaped white flower that blooms in a spike shape, and the fruit is fusiform and contains 5-6 seeds. Plantain seeds are oval, but they become sticky when immersed in water. This is a special ingredient and has been used in oriental medicine as anti-inflammatory, diuretic, and antitussive. In addition, the mucus from seeds is also used to increase the viscosity of noodles.
- the mugwort, moringa, bamboo, centella asiatica, pine and plantain can be used as leaves, flowers and stems, and it is particularly preferable to use leaves.
- mugwort extract, moringa extract, bamboo extract, centella extract, pine extract and plantain extract are each added 2 to 5 times the amount (w / v) of distilled water at 90 to 110 °C 1 After extracting for 5 hours, it can be obtained by filtration.
- the tissue containing cypress extract according to the present invention is not particularly limited to its use, and has antimicrobial activity, particularly, anti-atopy and anti-vaginitis, and is useful not only for general antibacterial but also for anti-dermatitis and feminine cleansing. It can be used by anyone, young or old, and especially, it can be very useful not only for infants and women, but also for patients.
- the tissue containing cypress extract of the present invention does not contain chemical synthetic substances, so it is not only safe for the human body, but also Escherichia coli , Staphylococcus epidermidis , Staphylococcus aureus. ) And Propionibacterium acnes ( Propionibacterium acnes ), and exhibits excellent antibacterial activity against the vaginitis causative bacteria Candida albicans and Gardnerella vaginalis. Therefore, the tissue containing cypress extract according to the present invention has an effect on anti-atopy and anti-vaginitis, and is useful not only for general antibacterial use, but also for anti-dermatitis and female cleansing, so that it can be used by anyone of all ages.
- tissue containing cypress extract of the present invention can be very useful in hospitals or nursing homes since barking and itching do not occur even when contacted with the skin for a long time.
- FIG 1 shows the degree of sebum secretion inhibition after tissue treatment prepared according to the present invention.
- Cypress leaves were collected, 3 times the amount (w/v) of distilled water was added, and 1% (v/v) of salt was added, followed by extraction at 100° C. for 2 hours, and then filtered through filter paper to obtain a cypress extract.
- the leaves of the mugwort were collected, extracted with 3-fold amount (w/v) of distilled water, extracted for 2 hours at 100°C, and filtered through a filter paper to obtain a mugwort extract.
- Moringa leaves were collected and extracted with 3 times the amount (w/v) of distilled water at 100° C. for 2 hours, and then filtered through filter paper to obtain Moringa extract.
- bamboo leaves were collected and extracted with 3-fold amount (w/v) of distilled water at 100° C. for 2 hours, and then filtered through filter paper to obtain a bamboo extract.
- Centella asiatica leaves were collected and extracted with 3 times the amount (w/v) of distilled water at 100° C. for 2 hours, and then filtered through a filter paper to obtain a Centella asiatica extract.
- Pine leaves were collected and extracted with 3-fold amount (w/v) of distilled water at 100° C. for 2 hours, and then filtered through a filter paper to obtain a pine extract.
- the plantain leaves were collected and extracted with 3 times the amount (w/v) of distilled water at 100° C. for 2 hours, and then filtered through a filter paper to obtain a plantain extract.
- a tissue containing 30 parts by weight of cypress extract was prepared based on 100 parts by weight of the tissue fabric.
- Tissue comprising 30 parts by weight of cypress extract and one or more extracts selected from the group consisting of mugwort extract, moringa extract, bamboo extract, centella extract, pine extract and plantain extract based on 100 parts by weight of tissue fabric as shown in Table 1 below Was prepared.
- a general tissue containing no cypress extract was prepared.
- Nutrient broth (NB) was used as a liquid medium for Escherichia coli (KCTC 1039) and Staphylococcus epidermidis (KCTC 1917), and tryptic soy broth (TSB) was used as a liquid medium for Staphylococcus aureus (KCTC 1621), and Propionibacterium acnes ( KCTC 3314) used GAM, and the solid medium was used by adding agar to the liquid medium.
- the strains were cultured for each growth temperature condition in a BOD incubator.
- the discs coated with the tissues of Examples 1 to 8 and Comparative Examples 1 to 7 were placed on the solid agar medium coated with the test strains and cultured, and then the antibacterial activity was measured.
- the antimicrobial activity was measured using a diffusion method (Paper Disc Method), and the antibacterial activity was evaluated by comparing the size of the transparent ring around the disk, and the results are shown in Table 2 below.
- the degree of inhibition of sebum production was measured through the degree of inhibition of the formation of lipid droplets of sebaceous cells (human sebocyte, Cellprogen, USA). Specifically, sebum cells were seeded and cultured on a collagen-coated 24-well plate at 2 x 10 3 cells/well, and then the tissue was plated and treated with 20 nM of insulin to induce the differentiation of sebaceous cells. After incubation for 8 days after treatment, the lipids produced were measured through a fluorescence microscope after staining the fat with nile red, which stains the red fat in the cells.
- Figure 1 shows the degree of sebum secretion inhibition after treatment with a cypress extract-containing tissue according to the present invention.
- THP-1 cells Human acute monocytic leukemia cells (THP-1 cells) were treated with antibiotics (penicillinm 10 4 U/ml, streptomycin 10 mg/ml, amphotericin B 25 ⁇ g/ml) and FBS (fetal bovine serum) 10. Using RPMI 1640 medium containing% (v/v), it was cultured for 3 days at 37° C. and 5% CO 2 .
- antibiotics penicillinm 10 4 U/ml, streptomycin 10 mg/ml, amphotericin B 25 ⁇ g/ml
- FBS fetal bovine serum
- the THP-1 cells were dispensed into a 24-well plate at a concentration of 2.0 x 10 6 ml using trypsin-EDTA, and the FBS was 0.5 Incubated for 16 hours at 37°C and 5% CO 2 in RPMI containing% (v/v).
- the tick extract (1 ⁇ g / ml) was treated as an atopic inducer in each well, and at the same time, the tissues of Examples 1 to 8 and Comparative Examples 1 to 7 were treated with DMSO (dimethyl sulfoxide) for 14 hours, and then ELISA (enzyme-linked immunospecific assay; Cytokine.
- Candida albicans (Candida albicans) was dispensed into a plate medium for measuring the disk diffusion method (Sabouraud Dextrose Agar. Difco.USA) by 100 ⁇ l and smeared, and then the tissue was placed on a sterilized paper disk.
- an antifungal agent Itraconazol
- Itraconazol was dropped onto a paper disk at a concentration of 100 ⁇ g/disk and then placed on a medium. After culturing the plate medium at 28° C. for 48 hours, the size of the growth inhibition ring (Clean Zone) was measured. The results are shown in Table 4 below.
- the tissues containing cypress extracts of Examples 1 to 8 of the present invention exhibited a strong growth inhibition effect compared to the tissues of Comparative Examples 1 to 7 as well as itraconazole as a control.
- the pre-cultured Gardnerella vaginalis was dispensed into a plate medium for disc diffusion measurement (Reinforced clostridial medium. Difco.USA) by 100 ⁇ l and spread, and then the tissue was placed on a sterilized paper disc.
- the antibiotic Ampicillin was dropped onto a paper disk at a concentration of 10 ⁇ g/disk, and then placed on the medium. This plate medium was placed at 37°C for 48 hours.
- Example 1 Candida albicans Gardnerella Vaginalis Control 30 31 Example 1 24 23 Example 2 27 26 Example 3 26 26 Example 4 25 26 Example 5 27 25 Example 6 26 25 Example 7 26 26 26 Example 8 32 32 Comparative Example 1 - - Comparative Example 2 One One Comparative Example 3 2 2 Comparative Example 4 2 2 Comparative Example 5 2 One Comparative Example 6 One 2 Comparative Example 7 2 2
- Example 5 Skin irritation test of tissue A skin irritation experiment was conducted on 10 healthy adults (5 males and 5 females) that meet the criteria for selecting subjects, have a healthy body and are between the ages of 20 and 40, The upper arm of the test subject was washed with 70% alcohol, and the tissue of Example 1 was closed.
- Test method The patch was applied on the applied area for 24 hours/48 hours, removed, and the skin irritation level was evaluated after 20 minutes.
- the present invention can be used in the industry related to the anti-atopy and anti-vaginitis tissue containing a cypress extract and a method for producing the same.
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Abstract
The present invention relates to anti-atopy and anti-vaginitis tissue containing a cypress extract and a preparation method thereof. Cypress extract-containing tissue of the present invention does not comprise a chemical synthesis material and thus is safe for the body, exhibits antimicrobial activities against Escherichia coli, Staphylococcus epidermidis, Staphylococcus aureus and Propionibacterium acnes, and also exhibits excellent antimicrobial activities against Candida albicans and Gardnerella vaginalis which are causative bacteria of vaginitis. Therefore, cypress extract-containing tissue according to the present invention has anti-atopy and anti-vaginitis effects and thus can be utilized for a general antimicrobial use as well as anti-skin inflammation and female hygiene, can be used by men and women of all ages and is particularly useful for infants, women and patients.
Description
본 발명은 편백 추출물을 함유하는 항아토피 및 항질염용 티슈 및 이의 제조방법에 관한 것으로, 보다 상세하게는 티슈에 화학적 방부제나 화학적 유화제를 따로 넣지 않으면서 항아토피 및 항질염 효능을 가지는 편백 추출물을 유효성분으로 포함하는 티슈 및 이의 제조방법에 관한 것이다. The present invention relates to an anti-atopy and anti-vaginitis tissue containing a cypress extract and a method for preparing the same, and more particularly, a cypress extract having anti-atopic and anti-vaginitis effects without separately adding a chemical preservative or chemical emulsifier to the tissue. It relates to a tissue containing as an active ingredient and a method of manufacturing the same.
오늘날의 티슈 및 화장지는 소비자의 다양화, 개성화 요구로 다습한 환경 및 외부 환경에 노출되어 사용되는 경우가 증가함에 따라 각종 균류가 그 표면과 내부에 발생하여 위생상 유해한 경우들이 발생할 수 있다. 예를 들면 주방용 타올, 화장실용 화장지 등은 수분이 많은 곳에서 주로 사용됨으로써, 셀룰로오스와 같은 종이 성분들이 곰팡이, 세균, 진균 등의 미생물에 의한 공격을 받기 쉽고 종이 노화 및 품질의 저하를 초래한다. As today's tissues and toilet papers are exposed to high humidity and external environments due to consumers' diversification and personalization demands, various fungi may be generated on the surface and inside of the tissues, which may be harmful to hygiene. For example, kitchen towels and toilet papers are mainly used in places with high moisture, so that paper components such as cellulose are susceptible to attack by microorganisms such as mold, bacteria, and fungi, leading to paper aging and deterioration of quality.
근래에 신종플루, 메르스 등의 감염성 질병으로 인한 개인위생 중요성이 강조되고 있는 기운데, 사람의 피부 및 손에 의한 세균 및 바이러스의 감염에 의해 대부분의 질병이 발병됨에 따라 물티슈 시장이 폭발적으로 성장하고 있으며, 특히 환절기마다 찾아오는 감기, 독감, 황사로 인해 항균 물티슈에 대한 요구가 증가하고 있다.In recent years, the importance of personal hygiene due to infectious diseases such as H1N1 flu and MERS has been emphasized. As most diseases are caused by bacterial and viral infections caused by human skin and hands, the wet tissue market has grown explosively. In particular, the demand for antibacterial wipes is increasing due to the cold, flu, and yellow dust that come each season.
일반적으로 피부에 상주하는 상재성 세균으로는 표피포도상구균(Staphylococcus epidermis)과 황색포도상구균(Staphylococcus aureus) 등이 있다. 표피포도상구균은 구균과에서 가장 잘 알려진 종류로 인간과 다른 항온동물의 피부와 점질피막에 보편적으로 존재하며, 일반적으로는 병원성이 없으나 저항력이 약해진 사람에게 나타나는 감염위험이 있다. 황색포도상구균은 상처감염ㅇ종기ㅇ피부감염의 중요한 병원균이며 식중독의 가장 일반적인 원인 가운데 하나로서, 건강한 사람의 30 ~ 50%에서도 발견되며 화농성 상청, 침 등에도 존재할 수 있기 때문에 개인위생에 소홀히 한 경우 오염될 수 있다.In general, epidermal bacteria residing in the skin include Staphylococcus epidermis and Staphylococcus aureus. Epidermal Staphylococcus aureus is the most well-known type in the aureus family, and it is commonly present in the skin and mucous membranes of humans and other warm-blooded animals, and is generally not pathogenic, but there is a risk of infection appearing in people with weakened resistance. Staphylococcus aureus is an important pathogen for wound infections, boils, and skin infections, and is one of the most common causes of food poisoning. It is also found in 30 to 50% of healthy people. It can also exist in purulent supernatant and saliva, so if personal hygiene is neglected It can be contaminated.
피부 자극의 화학적 및 환경적 원인에 더하여, 많은 사람들은 피부 자극제에 대한 타고난 민감성 또는 유전적인 소양을 갖는다. 자극의 정확한 원인이 무엇이든지 간에, 자극을 일으키는 경향이 있는 화학물질의 식별과 제품으로부터 그 성분을 제거함으로써 국소용 제품의 자극 잠재력을 감소시키려는 많은 시도가 있었다. 불행히도, 자극적인 화학물질 모두를, 특히 자극적인 화학물질이 제품의 활성 성분이거나 제형, 보존제 또는 기타의 기능성이 이유로 인해 필요한 것일 때, 이를 식별하거나 또는 제거하는 것은 종종 가능하지 않거나 실용적이지 않다. 따라서 피부 자극을 방지하거나 감소시키는 조성물 및 방법에 대한 연구가 필요하다.In addition to the chemical and environmental causes of skin irritation, many people have an innate sensitivity or genetic pruritus to skin irritants. Whatever the exact cause of the irritation, many attempts have been made to reduce the irritation potential of topical products by identifying chemicals that are prone to irritation and removing their components from the product. Unfortunately, it is often not possible or practical to identify or eliminate all of the irritating chemicals, especially when the irritating chemicals are the active ingredients of the product or are needed for reasons of formulation, preservative or other functionality. Therefore, there is a need for research on compositions and methods for preventing or reducing skin irritation.
한편, 아토피 (Atopic dermatitis)는 주로 유아기 혹은 소아기에 시작되는 만성적이고 재발성의 염증성 피부질환으로 가려움증과 피부 건조증 특징적인 습진을 동반한다. 유아기에는 얼굴과 팔 다리의 펼쳐진 쪽 부분에 습진으로 시작되지만, 성장하면서 특징적으로 팔이 굽혀지는 부분과 무릎 뒤의 굽혀지는 부위에 습진의 형태로 나타나게 되며, 많은 경우에 성장하면서 자연히 호전되는 경향을 보인다. 어른의 경우 접히는 부위 피부가 두꺼워지는 태선화가 나타나고, 유소아기에 비해 얼굴에 습진이 생기는 경우가 많다. 아토피의 발병원인은 아직 확실하게 알려져 있지 않은 상태이다. 임상 증상도 피부건조증, 습진 등으로 다양하게 나타나기 때문에 발병 원인이 어느 한가지로만 설명될 수는 없지만, 환경적인 요인과 유전적인 소인, 면역학적 반응 및 피부보호막의 이상 등이 주요 원인으로 여겨지고 있다. 환경적인 요인으로는 산업화로 인한 매연 등 환경 공해, 식품 첨가물 사용의 증가, 카펫, 침대, 소파의 사용으로 인한 진드기 등의 알레르기를 일으키는 원인 물질의 증가 등이 있다.On the other hand, atopic dermatitis is a chronic and recurrent inflammatory skin disease that begins mainly in infancy or childhood, and is accompanied by itching and dry skin characteristic eczema. In infancy, it starts with eczema on the spread side of the face and limbs, but as it grows, it appears in the form of eczema on the bending part of the arm and the bend behind the knee. In many cases, the tendency to improve naturally as it grows. see. In adults, the skin on the folds is thickened, and the skin is thickened, and eczema on the face is more common than in childhood. The cause of atopy is still unknown. Since clinical symptoms also appear in various ways such as dry skin and eczema, the cause of the onset cannot be explained with only one, but environmental factors, genetic predisposition, immunological reactions, and abnormal skin barriers are considered the main causes. Environmental factors include environmental pollution such as smoke caused by industrialization, increased use of food additives, and increased substances that cause allergies such as ticks due to the use of carpets, beds, and sofas.
한편, 여성의 질은 외음부와 자궁의 연결 통로로 여성의 몸에서 가장 습기가 많고 따뜻한 곳이다. 따라서 질염의 주원인인 세균이나 곰팡이가 서식하기 적합한 환경이므로 그로 인한 질환이 발생할 가능성이 크다. 정상적인 조건에서는 질 내부에 많은 유산균이 서식하면서 질 내부를 약산성으로 만들어 병원균이나 곰팡이가 증식하는 것을 억제하지만, 항생제 장기복용이나 피임약 장기복용, 탐폰이나 루프사용 또는 임신이나 출산, 수유, 폐경 등으로 여성 호르몬의 균형 변화 등의 이유로 질 내부의 균형이 깨어질 때 질 내에서 정상적인 조건에서는 성장이 통상적으로 억제되는 기회감염성 병원체의 증식이 일어나 질염이 발병할 수 있다. 질염은 세균성 질염(bacterial vaginosis), 칸디다성 질염(Candida vaginitis), 트리코모나스 질염(Trichomonas vaginitis), 위축성 질염 (Atrophic vaginitis)등이 보고되어 있다. 가장 흔한 질환은 세균성 질염(Bacterial vaginosis)으로, 가드네렐라 바지날리스 (Gardnerella vaginalis)에 의해 발병한다. 이 질염은 질 내 산도의 불균형으로 질 내에 서식하는 유산균이 줄어드는 반면 1%미만으로 존재하던 혐기성 세균이 과증식 하여 발생한다. 정상 질 세균총의 변화를 일으키는 유발인자에 관해서는 알려져 있지 않으나, 잦은 성교나 질 세척으로 질의 반복되는 알칼리성화가 그 역할을 하는 것으로 추정되고 있다.On the other hand, a woman's vagina is the most humid and warm place in a woman's body as a passage between the vulva and the uterus. Therefore, since it is an environment suitable for bacteria or fungi, which are the main causes of vaginitis, there is a high possibility of disease. Under normal conditions, many lactobacilli inhabit the vagina and make the vagina weakly acidic to suppress the growth of pathogens or fungi, but women who take antibiotics for a long time, take contraceptives for a long time, use tampons or loops, or are pregnant, childbirth, lactation, menopause, etc. When the balance of the vagina is broken due to changes in the balance of hormones, vaginitis may occur due to the proliferation of opportunistic pathogens that normally inhibit growth under normal conditions in the vagina. As vaginitis, bacterial vaginosis, Candida vaginitis, Trichomonas vaginitis, and atrophic vaginitis have been reported. The most common disease is bacterial vaginosis, which is caused by Gardnerella vaginalis. This vaginitis is caused by overgrowth of anaerobic bacteria that existed in less than 1% while the number of lactic acid bacteria in the vagina decreases due to an imbalance in vaginal acidity. Although the causative factor that causes changes in the normal vaginal flora is not known, it is estimated that repeated alkalizing of the vagina with frequent intercourse or vaginal washing plays a role.
정상적으로 있어야 할 과산화수소를 생성하는 유산균이 소실되면 정상 질 세균총의 재생은 어려워지기 때문에 세균성 질염의 재발이 자주 일어난다. 또한 세균성 질염을 가진 여성은 골반내 감염 (Pelvic inflammatory disease, PID), 유산 후 PID, 자궁 절제술 후 질정염(postoperative cuff cellulitis), 비정상 자궁경부 세포진 등 심각한 후유증의 빈도가 높아지며 임산부는 조기 양막파수, 조기진통, 조산, 융모양막염(chorioamnionitis), 제왕절개술 후 자궁내막염 등의 위험이 있다.When the lactic acid bacteria that produce hydrogen peroxide, which should normally be present, are lost, the regeneration of the normal vaginal flora becomes difficult, so recurrence of bacterial vaginosis often occurs. In addition, women with bacterial vaginosis have a higher frequency of serious sequelae, such as pelvic inflammatory disease (PID), post-abortion PID, postoperative cuff cellulitis, and abnormal cervical papillae. There is a risk of premature labor, preterm labor, chorioamnionitis, and endometritis after cesarean section.
세균성 질염과 함께 흔한 질환인 칸디다성 질염 (Candida vaginitis)은 진균인 칸디다 알비칸스 (Candida albicans)균이 습한 음부에 서식하면서 염증을 일으키는 질환으로서, 약 75%의 여성이 살아가는 동안 한 번 이상 질과 외음부의 칸디다성 질염을 겪으며 45%의 여성이 1년에 2회 이상의 재발을 겪는 흔한 질환이다. Candida vaginitis, a common disease along with bacterial vaginitis, is a disease in which the fungus Candida albicans lives in the moist vagina and causes inflammation.About 75% of women live with the vagina more than once during their lives. It is a common disease in which 45% of women suffer from candidal vaginitis of the vulva and have more than two recurrences per year.
항균성 티슈와 관련된 특허문헌으로 대한민국특허공개번호 제1997-0025529호에는 천연 활성 식물추출물을 함유한 일회용 물티슈 제품이 기재되어 있는데, 보다 구체적으로, 혈액순환, 염증억제, 수렴작용, 자극억제, 항균효과, 창상치유, 대사촉진 등에 우수한 효과를 가지고 있는 백리향(Thyme), 금잔화(Pot Marigold), 당귀(Thouki), 감초(Licorice root), 생강(Ginger), 작약(Shakuyaku), 참피나무(Lime tree), 알로에(Aloe), 로즈마리(Rosemary), 마늘(Galic), 귀리(Oats)의 천연 활성 식물주출물 1종 이상을 선택 첨가하여 함유시킨 일회용 물티슈 제품이 기재되어 있다.As a patent document related to antimicrobial tissue, Korean Patent Publication No. 1997-0025529 describes a disposable wet tissue product containing natural active plant extracts. More specifically, blood circulation, inflammation suppression, astringent effect, irritation suppression, antibacterial effect Thyme, Pot Marigold, Angelica (Thouki), Licorice root, Ginger, Shakuyaku, Lime tree, which have excellent effects on wound healing, metabolism, etc. , Aloe, rosemary, garlic (Galic), oats (Oats) are described a disposable wet tissue product containing one or more naturally active plant extracts.
대한민국특허공개번호 제2014-0068709호에는 아로마 에센셜 오일의 조성물 및 이를 이용한 물티슈가 기재되어 있는데, 보다 구체적으로, 조성물 총 중량에 대하여 라벤더 0.005 내지 0.02중량%, 로즈마리 0.001 내지 0.01중량%, 일랑일랑 0.001 내지 0.01중량%, 저먼 캐모마일 0.001 내지 0.01중량%, 로먼 캐모마일 0.001 내지 0.01중량%, 타임 0.001 내지 0.01중량% 및 티트리 0.005 내지 0.03중량%을 함유하는 아로마 에센셜 오일의 조성물 및 이를 이용한 물티슈가 기재되어 있다.Korean Patent Publication No. 2014-0068709 describes a composition of an aroma essential oil and a wet tissue using the same, and more specifically, 0.005 to 0.02% by weight of lavender, 0.001 to 0.01% by weight of rosemary, and 0.001 of ylang-ylang based on the total weight of the composition. A composition of an aroma essential oil containing from to 0.01% by weight, from 0.001 to 0.01% by weight of German chamomile, from 0.001 to 0.01% by weight of Roman chamomile, from 0.001 to 0.01% by weight of thyme, and from 0.005 to 0.03% by weight of tea tree, and a wet tissue using the same are described. have.
또한, 대한민국특허등록번호 제10-055045호에는 프로폴리스 추출액, 녹차 추출액 및 에탄올을 포함하는 위생용 물티슈가 기재되어 있는데, 보다 구체적으로, 위생용 물티슈에 155,000cc 내지 158,000cc의 정제수(purified water)에 대해 5,000cc 내지 500c의 에탄올(ethanol)과, 50g 내지 150g 의 녹차 추출물(Green tea extract)와, 80g 내지 200g의 프로폴리스(Propolis extract)를 포함하는 물티슈로서, 휴대가 간편하고 주방, 유아, 애완견 또는 환자의 신체의 일부를 닦는데 용이한 위생용 물티슈가 기재되어 있다.In addition, Korean Patent Registration No. 10-055045 describes a sanitary wipe including propolis extract, green tea extract, and ethanol, and more specifically, 155,000 cc to 158,000 cc of purified water for sanitary wipes. About 5,000cc to 500c of ethanol (ethanol), 50g to 150g of green tea extract (Green tea extract), and 80g to 200g of propolis (Propolis extract) as a wet tissue, it is easy to carry, kitchen, infant, Hygienic wipes are described that are easy to clean for dogs or parts of the patient's body.
또한, 대한민국특허공개번호 제2003-0017845호에는 게르마늄과 어성초를 함유한 물티슈 및 그 제조방법이 기재되어 있는데, 보다 구체적으로, 피부보호 및 피부질환 치료제로 쓰이는 한방 생약성분이 함유된, 직포 및/또는 펄프로된 원단에, 죽염 및 자몽추출물과, 게르마늄과, 어성초가 함유되어 있는 물티슈로서, 피부안전성, 피부보습효과 및 살균효과가 우수하여, 부작용이 전혀 없이 피부를 건강하게 하며 피부미용효과를 높이는 효과를 갖는 물티슈가 기재되어 있다.In addition, Korean Patent Publication No. 2003-0017845 describes a wet tissue containing germanium and Eoseongcho and a method for manufacturing the same.More specifically, a woven fabric containing herbal herbal ingredients used as skin protection and treatment for skin diseases is described. Or, as a wet tissue containing bamboo salt and grapefruit extract, germanium and Eoseongcho in a pulp fabric, it has excellent skin safety, skin moisturizing effect and sterilization effect, making the skin healthy without any side effects and enhancing skin beauty effects. Wet wipes having a heightening effect are described.
또한, 대한민국특허등록번호 제10-1626575호에는 친환경 항균제를 포함하는 아토피 예방용 항균 물티슈가 기재되어 있는데, 보다 구체적으로, 물티슈 원단에 물티슈용 조성물을 함침하여 제조하되, 물티슈용 조성물은 정제수; 보습제; 계면활성제; pH조절제; 에센셜 오일(essential oil) 및 항균제;를 포함하고, 항균제는 어성초 추출물, 자소엽 추출물, 은행잎 추출물, 소라쟁이 추출물, 계피 추출물, 산초 추출물, 옻나무 뿌리 추출물 및 백두옹 추출물을 포함하는, 인체에 무해하면서도 우수한 항균, 탈취, 아토피 예방 효과를 가지는 물티슈용 항균 조성물을 포함하는 항균 물티슈가 기재되어 있다.In addition, Korean Patent Registration No. 10-1626575 describes an antibacterial wipe for preventing atopy containing an eco-friendly antimicrobial agent. More specifically, it is prepared by impregnating a wet tissue fabric with a composition for wet tissue, but the composition for wet tissue is purified water; Moisturizer; Surfactants; pH regulator; Essential oil (essential oil) and an antibacterial agent; Including, the antibacterial agent is harmless to the human body, but excellent, including Eoseongcho extract, perilla leaf extract, ginkgo leaf extract, conch extract, cinnamon extract, Japanese pepper extract, sumac root extract and baekduong extract Antibacterial wipes comprising an antibacterial composition for wet wipes having antibacterial, deodorant, and atopy prevention effects are disclosed.
이와 같은 종래기술을 살펴보면, 여러 가지 약초를 함께 넣어 추출하는 방식 등이 기재되어 있으나, 여전히 합성 원료를 사용하고 있어 피부를 상하게 하거나 순수 약초만이 사용될 경우 방부 및 항균 효과가 떨어지는 문제점을 안고 있다. Looking at such a prior art, a method of adding and extracting various herbs together is described, but since synthetic raw materials are still used, there is a problem in that the antiseptic and antibacterial effects are inferior when only pure herbs are used or damaged the skin.
이에 본 발명자들은 천연 물질을 함유함으로써 인체에 안전하며, 일반 세균과 아토피 및 여드름 관련 균뿐만 아니라 여성질염과 관련된 균에 대한 항균활성을 가지는 티슈를 개발하기 위해 계속 연구를 진행하던 중 편백 추출물을 첨가한 티슈가 일반 세균뿐만 아니라 아토피 관련 균주 및 여성질염 관련 균주에 대한 저해활성을 가진다는 사실을 발견함으로써 본 발명을 완성하였다. Accordingly, the inventors of the present invention added cypress extract while continuing research to develop tissues that are safe for the human body by containing natural substances and have antibacterial activity against bacteria related to female vaginitis as well as general bacteria and atopic and acne-related bacteria. The present invention was completed by finding that a tissue has inhibitory activity against not only general bacteria, but also atopy-related strains and female vaginitis-related strains.
따라서, 본 발명에서 해결하고자 하는 기술적 과제는 화학합성물질을 포함하지 않으면서 항아토피 및 항질염 효능이 우수한 티슈를 제공하고자 한다.Therefore, the technical problem to be solved in the present invention is to provide a tissue having excellent anti-atopy and anti-vaginitis efficacy without containing a chemical synthetic substance.
또한, 본 발명에서 해결하고자 하는 다른 기술적 과제는 상기 티슈의 제조방법을 제공하기 위한 것이다.In addition, another technical problem to be solved in the present invention is to provide a method for manufacturing the tissue.
상기한 기술적 과제를 해결하기 위하여, 본 발명에서는 편백 추출물을 유효성분으로 포함하는 것을 특징으로 하는 항아토피 및 항질염용 티슈를 제공한다.In order to solve the above technical problem, the present invention provides an anti-atopic and anti-vaginitis tissue, characterized in that it contains a cypress extract as an active ingredient.
바람직하게, 본 발명에 따른 항아토피 및 항질염용 티슈는 티슈 원단 100 중량부에 대하여 편백 추출물 1 내지 40 중량부를 포함하는 것을 특징으로 한다.Preferably, the anti-atopy and anti-vaginitis tissue according to the present invention is characterized in that it contains 1 to 40 parts by weight of cypress extract based on 100 parts by weight of the tissue fabric.
바람직하게, 본 발명에 따른 항아토피 및 항질염용 티슈는 쑥 추출물, 모링가 추출물, 대나무 추출물, 병풀 추출물, 소나무 추출물 및 질경이 추출물로 이루어진 군에서 선택된 하나 이상의 추출물을 티슈 원단 100 중량부에 대하여 1 내지 20 중량부의 양으로 포함하는 것을 특징으로 한다.Preferably, the tissue for anti-atopy and anti-vaginosis according to the present invention comprises at least one extract selected from the group consisting of mugwort extract, moringa extract, bamboo extract, centella extract, pine extract and plantain extract per 100 parts by weight of tissue fabric 1 It characterized in that it contains in an amount of to 20 parts by weight.
상기한 다른 기술적 과제를 해결하기 위하여, 본 발명에서는 티슈 원단 100 중량부에 대하여 편백 추출물을 1 내지 40 중량부의 양으로 혼합하는 것을 특징으로 하는 항아토피 및 항질염용 티슈의 제조방법을 제공한다.In order to solve the above-described other technical problem, the present invention provides a method for preparing a tissue for anti-atopy and anti-vaginitis, characterized in that the cypress extract is mixed in an amount of 1 to 40 parts by weight based on 100 parts by weight of the tissue fabric.
본 발명에서 티슈는 건티슈 또는 함수티슈로서 물티슈일 수 있으며, 항균활성, 특히 항아토피 및 항질염 효능을 가지는 바, 일반적인 항균용뿐만 아니라 항피부염증용, 여성청결용으로 모두 유용하여 남녀노소 누구든지 사용가능하며, 특히 갓난아기, 여성뿐만 아니라 환자용으로도 매우 유용하게 사용될 수 있다.In the present invention, the tissue may be a dry tissue or a wet tissue, and it has antibacterial activity, especially anti-atopic and anti-vaginitis effects, and is useful for both general antibacterial, anti-dermatitis, and feminine cleansing. Can be used, especially for infants, women, as well as patients, can be very useful.
또한, 본 발명의 편백 추출물 함유 티슈는 오랜시간 피부에 접촉시키더라도 짖무름 및 간지러움이 발생하지 않아 병원 또는 요양원에서도 매우 유용하게 사용될 수 있다.In addition, the tissue containing cypress extract of the present invention can be very useful in hospitals or nursing homes since barking and itching do not occur even when contacted with the skin for a long time.
상기 티슈 원단은 목재펄프로서 통상적으로 티슈에 사용되는 천연목재 또는 합성목재 펄프일 수 있으며, 이에 특별한 제한은 없다. The tissue fabric may be a natural wood or synthetic wood pulp that is commonly used for tissues as wood pulp, and there is no particular limitation thereto.
본 발명에서 질염은 칸디다성 질염인 것으로서, 칸디다성 질염의 원인균이 칸디다 알비칸스 (Candida albicans)인 것일 수 있으며, 또한 세균성 질염인 것으로서, 세균성 질염의 원인균인 가드네렐라 바지날리스(Gardnerella vaginalis)인 것일 수 있다.In the present invention, vaginitis is Candidal vaginitis, and the causative agent of Candida albicans may be Candida albicans, and also as a bacterial vaginosis, Gardnerella vaginalis, a causative agent of bacterial vaginosis. It can be.
본 발명의 편백 추출물 함유 티슈는 대장균(Escherichia coli), 스타필로코커스 에피데르미스(Staphylococcus epidermidis), 스타필로코커스 아우레우스(Staphylococcus aureus) 및 프로피오니박테리움 아크네스(Propionibacterium acnes)에 대한 항균력을 나타내며, 질염 원인균인 칸디다 알비칸스 (Candida albicans) 및 가드네렐라 바지날리스(Gardnerella vaginalis)에 대하여도 우수한 항균을 나타낸다. The tissue containing cypress extract of the present invention exhibits antimicrobial activity against Escherichia coli , Staphylococcus epidermidis , Staphylococcus aureus , and Propionibacterium acnes . It also exhibits excellent antimicrobial against Candida albicans and Gardnerella vaginalis, which are vaginitis causative bacteria.
본 발명의 유효성분인 편백은 세균에 대한 항균 및 살균 작용이 뛰어나 웰빙용품 소재로 많이 사용되며, 편백나무에는 피톤치드라는 천연 항균물질이 많이 함유되어 있어 살균 작용이 뛰어나고, 내수성이 강해 물에 닿으면 고유의 향이 진하게 퍼져 잡냄새도 없애주기 때문에 최근 도마 재질로 많이 사용되고 있다.Cypress, an active ingredient of the present invention, has excellent antibacterial and sterilizing effects against bacteria and is widely used as a material for well-being. Cypress wood contains a lot of natural antibacterial substances called phytoncide, so it has excellent sterilization effect and strong water resistance when it comes into contact with water. It is widely used as a cutting board material in recent years because its unique scent spreads deeply to remove odors.
본 발명에 있어서, "추출물"은 각 생약을 통상의 방법에 의하여 추출한 추출물로서, 생약을 추출한 추출액뿐만 아니라 이의 건조 분말 또는 이를 이용하여 제형화된 모든 형태를 포함한다.In the present invention, "extract" is an extract obtained by extracting each herbal medicine by a conventional method, and includes not only the extract from which the herbal medicine is extracted, but also a dry powder thereof or any form formulated using the same.
본 발명의 하나의 구현예에 따르면, 편백 추출물은 2 내지 5배 양(w/v)의 증류수를 넣어 90 내지 110℃에서 1 내지 5시간 추출한 후 여과하여 수득할 수 있다.According to one embodiment of the present invention, the cypress extract can be obtained by adding 2 to 5 times the amount (w/v) of distilled water and extracting at 90 to 110° C. for 1 to 5 hours and then filtering.
상기 편백은 잎, 꽃 및 줄기 모두 사용가능하며, 특히 잎을 사용하는 것이 바람직하며, 이 때 꽃봉우리는 제외하고 사용한다.The cypress can be used for both leaves, flowers and stems, and it is particularly preferable to use leaves, excluding flower peaks.
구체적으로, 상기 편백 추출물은 잎을 3배 양(w/v)의 증류수를 넣고 1%(v/v)의 소금을 넣고 110℃에서 2시간 동안 추출한 후 여과하여 수득할 수 있다.Specifically, the cypress extract can be obtained by adding 3 times the amount (w/v) of distilled water to the leaves, adding 1% (v/v) of salt, extracting at 110° C. for 2 hours, and filtering.
상기 추출 방법은 제한되지 않고, 예를 들어, 냉침추출, 초음파 추출, 환류 냉각 추출 등이 있다.The extraction method is not limited, for example, cold sediment extraction, ultrasonic extraction, reflux cooling extraction, and the like.
본 발명의 편백 추출물은 추출, 분획, 또는 정제(분리, 분획)의 각 단계에서 얻어지는 모든 추출액, 분획, 정제물, 그들의 희석액, 농축액, 또는 건조물일 수 있다.The cypress extract of the present invention may be all extracts, fractions, purified products, diluted solutions, concentrates, or dried products obtained in each step of extraction, fractionation, or purification (separation, fractionation).
본 발명의 하나의 구현예에 따르면, 항아토피 및 항질염용 티슈는 티슈 원단 100 중량부에 대하여 편백 추출물 1 내지 40 중량부, 바람직하게는 10 내지 20 중량부를 포함하는 것을 특징으로 한다. 이 때 편백 추출물이 1 중량부 미만으로 사용되는 경우 항아토피 및 항질염 효과를 수득할 수 없으며, 편백 추출물이 40 중량부 초과로 사용되면 편백향이 너무 과도하여 오히려 거부감이 일 수 있다.According to one embodiment of the present invention, the anti-atopy and anti-vaginitis tissue is characterized in that it contains 1 to 40 parts by weight, preferably 10 to 20 parts by weight, of cypress extract based on 100 parts by weight of the tissue fabric. At this time, when the cypress extract is used in an amount of less than 1 part by weight, anti-atopic and anti-vaginitis effects cannot be obtained, and when the cypress extract is used in an amount exceeding 40 parts by weight, the cypress flavor is too excessive, which may result in rejection.
본 발명의 하나의 구현예에 따르면, 본 발명의 항아토피 및 항질염용 티슈는 항아토피 및 항질염 효과를 보다 높이기 위하여, 쑥 추출물, 모링가 추출물, 대나무 추출물, 병풀 추출물, 소나무 추출물 및 질경이 추출물로 이루어진 군에서 선택된 하나 이상의 추출물을 티슈 원단 100 중량부에 대하여 1 내지 20 중량부의 양으로 포함하는 것을 특징으로 한다.According to one embodiment of the present invention, the anti-atopy and anti-vaginitis tissue of the present invention is to further increase the anti-atopy and anti-vaginitis effect, mugwort extract, moringa extract, bamboo extract, centella extract, pine extract and plantain extract It is characterized in that it contains at least one extract selected from the group consisting of 1 to 20 parts by weight based on 100 parts by weight of the tissue fabric.
구체적으로는 상기 쑥 추출물, 모링가 추출물, 대나무 추출물, 병풀 추출물, 소나무 추출물 및 질경이 추출물을 모두 티슈 원단에 첨가하는 것이 바람직하며, 이 때 쑥 추출물, 모링가 추출물, 대나무 추출물, 병풀 추출물, 소나무 추출물 및 질경이 추출물은 1:1:1:1:1:1 중량비로 혼합하여 첨가하는 것이 바람직하다.Specifically, the mugwort extract, moringa extract, bamboo extract, centella extract, pine extract, and plantain extract are all preferably added to the tissue fabric, and at this time, mugwort extract, moringa extract, bamboo extract, centella extract, pine extract And plantain extract is preferably added by mixing in a 1:1:1:1:1:1 weight ratio.
상기 쑥은 단군신화에 등장할 정도로 역사가 오래된 쑥은, 특유의 향을 내는 정유 성분인 '시네올'을 함유하고 있어 체내의 유해 세균 성장을 억제하고 면역과 해독작용에 뛰어난 효과가 있다. 음식의 재료뿐 아니라 차나 약재, 염색제, 화장품 등으로도 활용되는 등 쓰임새가 다양하다Mugwort, which has a long history to appear in the Dangun mythology, contains'cineol', an essential oil that gives off a unique scent, so it suppresses the growth of harmful bacteria in the body and has excellent effects on immunity and detoxification. It is used not only as a food ingredient, but also as a tea, medicinal material, dye, cosmetics, etc.
상기 모링가는 인도와 아프리카 등에서 자생하는 콩과 식물로서, 열대 지방 전역에서 재배하며 항산화 물질 등 영양소가 풍부해 슈퍼푸드의 하나로 주목받고 있다.The moringa is a legume plant that grows naturally in India and Africa, and is cultivated throughout the tropics and is attracting attention as one of the superfoods because it is rich in nutrients such as antioxidants.
상기 대나무는 단자엽식물이므로 나이테가 없고 비대생장을 하지 않는다. 줄기는 원통형이고 가운데가 비었다. 표면은 녹색에서 황록색으로 변하며 포엽은 일찍 떨어진다. 약용으로는 왕대나 솜대의 줄기 내부에 있는 막상피는 죽여라 하여 치열과 토혈에 사용하며, 왕대나 솜대에서 뽑아낸 대기름은 죽력이라 하여 고혈압에 쓰일 뿐 아니라 만병통치약으로 알려져 왔다. 또한, 죽엽은 치열, 이수, 청심제로 사용한다.Since the bamboo is a monocotyledonous plant, it does not have rings and does not grow hypertrophy. The stem is cylindrical and the center is empty. The surface changes from green to yellow-green, and the bracts fall early. For medicinal purposes, the membrane epithelium inside the stem of Wangdae or Somdae is said to be killed, and is used for dentition and hematopoiesis, and the air oil extracted from Wangdae or Somdae is used for high blood pressure and has been known as a panacea. In addition, bamboo leaves are used as a dentition, acupuncture, and cheongsim.
상기 병풀은 가장자리에 톱니를 두른 작은 부채 모양의 잎에 흰색 또는 연보라색의 꽃을 피우는 미나리과 식물이다. 열매는 작은 타원형으로, 끝에 튀어나온 그물눈이 있다. 따뜻하고 습기가 많은 지역에서 자라기 때문에, 우리나라에서는 제주도 등 남쪽 섬 지방의 산과 들에서 흔히 볼 수 있다. 인도에서는 상처를 입은 호랑이가 병풀이 많이 난 곳에서 뒹굴어 치료하는 것을 보고 호랑이풀이라고 부르며 오래 전부터 약으로 써 왔다. 병풀의 잎과 줄기에 있는 마데카식산이란 성분이 염증을 낫게 하고, 종양과 궤양 등의 상처를 치유하는 힘이 있다는 게 밝혀지면서 연고나 치약, 화장품 등의 원료로도 많이 쓰이고 있다.The Centella asiatica is a parsley family that blooms white or light purple flowers on small fan-shaped leaves covered with serrated edges. The fruit is small oval, with areolae protruding at the tip. Because it grows in warm and humid areas, it is commonly found in the mountains and fields of southern islands such as Jeju Island in Korea. In India, injured tigers have been used as medicine for a long time, calling them tiger grass after seeing them roll over and heal them in places with a lot of centella. Madecassic acid in the leaves and stems of Centella asiatica has been found to have the power to heal inflammation and to heal wounds such as tumors and ulcers, so it is widely used as a raw material for ointments, toothpaste, and cosmetics.
상기 소나무는 한국, 일본, 중국 등지에 분포되어 있는 침엽상록수다. 항상 푸르고 낙엽도 잘 지지 않는다. 줄기와 잎의 형태가 특이해 동양의 그림에 많이 등장하는데 '신이 하늘로부터 내려오는 것을 기다리는 나무'라는 전설도 있다. 소나무도 종류가 다양한데 잎이 3~5개씩 모여 나는 것은 왜송, 잎이 침형으로 2개씩 모여 나는 것은 조선솔이다. 조선솔은 또 적송과 흑송으로 나뉘는데 흑송은 해송이라 하여 바닷가에서 자라는 소나무로 약효가 뛰어나다.The pine tree is a coniferous evergreen tree distributed in Korea, Japan, and China. It is always green, and leaves do not resist well. Due to the unique shape of the stem and leaf, it appears in many oriental paintings, and there is also a legend that'a tree waiting for the god to come down from the sky'. There are also various types of pine trees. Three to five leaves are gathered together by Japanese pine, and two leaves are gathered in needle-like form by Chosun brush. Chosunsol is also divided into red pine and heuksong. Heuksong is called Haesong, and it is a pine tree that grows on the seashore and has excellent medicinal properties.
상기 질경이는 들판이나 한길 등 도처에서 가장 흔하게 볼 수 있는 잡초의 하나이다. 질경이의 한명은 차전초라고 하여 수레가 지나간 바퀴자리에 씨앗이 모여 수북이 나기 때문에 붙여진 이름이다. 우리나라 전국 각지에서 나고 일본, 대만, 중국, 시베리아, 말레이시아 등지에도 분포한다. 질경이과에 속하는 다년초로 잎은 난형 또는 타원형이다. 우리나라에는 갯질경이, 긴잎질경이, 왕질경이, 털질경이 등의 1속 10여종이 분포되어 있다. 화관이 깔때기 모양인 흰 꽃이 이삭 모양으로 피는데 열매는 방추형이고 씨앗이 5~6개 들어있다. 질경이 씨는 타원형인데 물에 담그면 끈끈하게 된다. 이것이 특수성분으로 한방에서는 소염, 이뇨, 진해제로 쓰여 왔다. 또한 종자의 점액은 국수의 점도를 높이는 데도 사용한다. The plantain is one of the most common weeds that can be seen everywhere, such as in a field or a han-gil. One of the plantains is called Chajeoncho, and it is named because the seeds gather in the seat of the wheel where the cart passed, and a drumstick is formed. It is found all over Korea and is also distributed in Japan, Taiwan, China, Siberia, and Malaysia. It is a perennial plant belonging to the plantain family, and its leaves are ovate or oval. In Korea, there are about 10 species in one genus, such as Psyllium Psyllium, Long Leaf Psyllium, Wang Psyllium, and Hair Psyllium. The corolla is a funnel-shaped white flower that blooms in a spike shape, and the fruit is fusiform and contains 5-6 seeds. Plantain seeds are oval, but they become sticky when immersed in water. This is a special ingredient and has been used in oriental medicine as anti-inflammatory, diuretic, and antitussive. In addition, the mucus from seeds is also used to increase the viscosity of noodles.
상기 쑥, 모링가, 대나무, 병풀, 소나무 및 질경이는 잎, 꽃 및 줄기 모두 사용가능하며, 특히 잎을 사용하는 것이 바람직하다.The mugwort, moringa, bamboo, centella asiatica, pine and plantain can be used as leaves, flowers and stems, and it is particularly preferable to use leaves.
본 발명의 하나의 구현예에 따르면, 쑥 추출물, 모링가 추출물, 대나무 추출물, 병풀 추출물, 소나무 추출물 및 질경이 추출물은 각각 2 내지 5배 양(w/v)의 증류수를 넣어 90 내지 110℃에서 1 내지 5시간 추출한 후 여과하여 수득할 수 있다.According to one embodiment of the present invention, mugwort extract, moringa extract, bamboo extract, centella extract, pine extract and plantain extract are each added 2 to 5 times the amount (w / v) of distilled water at 90 to 110 ℃ 1 After extracting for 5 hours, it can be obtained by filtration.
본 발명에 따른 편백 추출물 함유 티슈는 그 사용에 특별히 제한되지 않으며, 항균활성, 특히 항아토피 및 항질염에 대한 효능을 가지는 바, 일반적인 항균용뿐만 아니라 항피부염증용, 여성청결용으로 모두 유용하여 남녀노소 누구든지 사용가능하며, 특히 갓난아기, 여성뿐만 아니라 환자용으로도 매우 유용하게 사용될 수 있다. The tissue containing cypress extract according to the present invention is not particularly limited to its use, and has antimicrobial activity, particularly, anti-atopy and anti-vaginitis, and is useful not only for general antibacterial but also for anti-dermatitis and feminine cleansing. It can be used by anyone, young or old, and especially, it can be very useful not only for infants and women, but also for patients.
이와 같이, 본 발명의 편백 추출물 함유 티슈는 화학합성물질을 포함하지 않아 인체에 안전할 뿐만 아니라 대장균(Escherichia coli), 스타필로코커스 에피데르미스(Staphylococcus epidermidis), 스타필로코커스 아우레우스(Staphylococcus aureus) 및 프로피오니박테리움 아크네스(Propionibacterium acnes)에 대한 항균력을 나타내며, 질염 원인균인 칸디다 알비칸스 (Candida albicans) 및 가드네렐라 바지날리스(Gardnerella vaginalis)에 대하여도 우수한 항균을 나타낸다. 따라서, 본 발명에 따른 편백 추출물 함유 티슈는 항아토피 및 항질염에 대한 효능을 가지는 바, 일반적인 항균용뿐만 아니라 항피부염증용, 여성청결용으로 모두 유용하여 남녀노소 누구든지 사용가능하며, 특히 갓난아기, 여성뿐만 아니라 환자용으로도 매우 유용하게 사용될 수 있다. 또한, 본 발명의 편백 추출물 함유 티슈는 오랜시간 피부에 접촉시키더라도 짖무름 및 간지러움이 발생하지 않아 병원 또는 요양원에서도 매우 유용하게 사용될 수 있다.As described above, the tissue containing cypress extract of the present invention does not contain chemical synthetic substances, so it is not only safe for the human body, but also Escherichia coli , Staphylococcus epidermidis , Staphylococcus aureus. ) And Propionibacterium acnes ( Propionibacterium acnes ), and exhibits excellent antibacterial activity against the vaginitis causative bacteria Candida albicans and Gardnerella vaginalis. Therefore, the tissue containing cypress extract according to the present invention has an effect on anti-atopy and anti-vaginitis, and is useful not only for general antibacterial use, but also for anti-dermatitis and female cleansing, so that it can be used by anyone of all ages. It can be very useful not only for babies and women, but also for patients. In addition, the tissue containing cypress extract of the present invention can be very useful in hospitals or nursing homes since barking and itching do not occur even when contacted with the skin for a long time.
본 명세서에 첨부되는 다음의 도면들은 본 발명의 바람직한 실시예를 예시하는 것이며, 전술한 발명의 내용과 함께 본 발명의 기술사상을 더욱 이해시키는 역할을 하는 것이므로, 본 발명은 그러한 도면에 기재된 사항에만 한정되어 해석되어서는 아니 된다.The following drawings attached to the present specification illustrate preferred embodiments of the present invention, and serve to further understand the technical idea of the present invention together with the content of the above-described invention, so the present invention is limited to the matters described in such drawings. It is limited and should not be interpreted.
도 1은 본 발명에 따라 제조된 티슈 처리 후 피지분비 억제 정도를 보여주는 것이다.1 shows the degree of sebum secretion inhibition after tissue treatment prepared according to the present invention.
이하, 본 발명의 이해를 돕기 위하여 실시예 등을 들어 상세하게 설명하기로 한다. 그러나, 본 발명에 따른 실시예들은 여러 가지 다른 형태로 변형될 수 있으며, 본 발명의 범위가 하기 실시예들에 한정되는 것으로 해석되어서는 안 된다. 본 발명의 실시예들은 당업계에서 평균적인 지식을 가진 자에게 본 발명을 보다 완전하게 설명하기 위해 제공되는 것이다. Hereinafter, examples, etc. will be described in detail to aid understanding of the present invention. However, the embodiments according to the present invention may be modified in various forms, and the scope of the present invention should not be construed as being limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.
<제조예 1> 편백 추출물 제조<Production Example 1> Preparation of cypress extract
편백의 잎을 수집하여 3배 양(w/v)의 증류수를 넣고 1%(v/v)의 소금을 첨가하여 100℃에서 2시간 동안 추출한 다음 여과지로 여과하여 편백 추출물을 수득하였다.Cypress leaves were collected, 3 times the amount (w/v) of distilled water was added, and 1% (v/v) of salt was added, followed by extraction at 100° C. for 2 hours, and then filtered through filter paper to obtain a cypress extract.
<제조예 2> 쑥 추출물 제조<Production Example 2> Preparation of mugwort extract
쑥의 잎을 수집하여 3배 양(w/v)의 증류수를 넣어 100℃에서 2시간 동안 추출한 다음 여과지로 여과하여 쑥 추출물을 수득하였다.The leaves of the mugwort were collected, extracted with 3-fold amount (w/v) of distilled water, extracted for 2 hours at 100°C, and filtered through a filter paper to obtain a mugwort extract.
<제조예 3> 모링가 추출물 제조<Preparation Example 3> Preparation of Moringa extract
모링가의 잎을 수집하여 3배 양(w/v)의 증류수를 넣어 100℃에서 2시간 동안 추출한 다음 여과지로 여과하여 모링가 추출물을 수득하였다.Moringa leaves were collected and extracted with 3 times the amount (w/v) of distilled water at 100° C. for 2 hours, and then filtered through filter paper to obtain Moringa extract.
<제조예 4> 대나무 추출물 제조<Production Example 4> Preparation of bamboo extract
대나무 잎을 수집하여 3배 양(w/v)의 증류수를 넣어 100℃에서 2시간 동안 추출한 다음 여과지로 여과하여 대나무 추출물을 수득하였다.Bamboo leaves were collected and extracted with 3-fold amount (w/v) of distilled water at 100° C. for 2 hours, and then filtered through filter paper to obtain a bamboo extract.
<제조예 5> 병풍 추출물 제조<Production Example 5> Preparation of Byeongpung extract
병풀 잎을 수집하여 3배 양(w/v)의 증류수를 넣어 100℃에서 2시간 동안 추출한 다음 여과지로 여과하여 병풀 추출물을 수득하였다.Centella asiatica leaves were collected and extracted with 3 times the amount (w/v) of distilled water at 100° C. for 2 hours, and then filtered through a filter paper to obtain a Centella asiatica extract.
<제조예 6> 소나무 추출물 제조<Preparation Example 6> Preparation of pine extract
소나무 잎을 수집하여 3배 양(w/v)의 증류수를 넣어 100℃에서 2시간 동안 추출한 다음 여과지로 여과하여 소나무 추출물을 수득하였다.Pine leaves were collected and extracted with 3-fold amount (w/v) of distilled water at 100° C. for 2 hours, and then filtered through a filter paper to obtain a pine extract.
<제조예 7> 질경이 추출물 제조<Preparation Example 7> Preparation of plantain extract
질경이 잎을 수집하여 3배 양(w/v)의 증류수를 넣어 100℃에서 2시간 동안 추출한 다음 여과지로 여과하여 질경이 추출물을 수득하였다.The plantain leaves were collected and extracted with 3 times the amount (w/v) of distilled water at 100° C. for 2 hours, and then filtered through a filter paper to obtain a plantain extract.
<실시예 1> 편백 추출물 함유 티슈 제조<Example 1> Preparation of tissue containing cypress extract
티슈 원단 100 중량부에 대하여 편백 추출물 30 중량부를 포함하는 티슈를 제조하였다.A tissue containing 30 parts by weight of cypress extract was prepared based on 100 parts by weight of the tissue fabric.
<실시예 2 내지 실시예 8> <Examples 2 to 8>
하기 표 1에 나타낸 바와 같이 티슈 원단 100 중량부에 대하여 편백 추출물 30 중량부 및 쑥 추출물, 모링가 추출물, 대나무 추출물, 병풀 추출물, 소나무 추출물 및 질경이 추출물로 이루어진 군에서 선택된 하나 이상의 추출물을 포함하는 티슈를 제조하였다.Tissue comprising 30 parts by weight of cypress extract and one or more extracts selected from the group consisting of mugwort extract, moringa extract, bamboo extract, centella extract, pine extract and plantain extract based on 100 parts by weight of tissue fabric as shown in Table 1 below Was prepared.
<비교예 1> <Comparative Example 1>
편백 추출물을 포함하지 않는 일반 티슈를 제조하였다.A general tissue containing no cypress extract was prepared.
<비교예 2 내지 비교예 7> <Comparative Examples 2 to 7>
편백 추출물을 포함하지 않으면서 하기 표 1에 나타낸 바와 같이 티슈 원단 100 중량부에 대하여 쑥 추출물, 모링가 추출물, 대나무 추출물, 병풀 추출물, 소나무 추출물 및 질경이 추출물로 이루어진 군에서 선택된 하나의 추출물을 포함하는 티슈를 제조하였다.Containing one extract selected from the group consisting of mugwort extract, moringa extract, bamboo extract, centella extract, pine extract, and plantain extract based on 100 parts by weight of tissue fabric as shown in Table 1 below without containing cypress extract A tissue was prepared.
| 편백 추출물Cypress extract | 쑥 추출물Mugwort extract | 모링가 추출물Moringa extract | 대나무 추출물Bamboo extract | 병풀 추출물Centella asiatica extract | 소나무 추출물Pine extract | 질경이 추출물Plantain extract | |
| 실시예 1Example 1 | 30 중량부30 parts by weight | -- | -- | -- | -- | -- | -- |
| 실시예 2Example 2 | 30 중량부30 parts by weight | 12 중량부12 parts by weight | -- | -- | -- | -- | -- |
| 실시예 3Example 3 | 30 중량부30 parts by weight | -- | 12 중량부12 parts by weight | -- | -- | -- | -- |
| 실시예 4Example 4 | 30 중량부30 parts by weight | -- | -- | 12 중량부12 parts by weight | -- | -- | -- |
| 실시예 5Example 5 | 30 중량부30 parts by weight | -- | -- | -- | 12 중량부12 parts by weight | -- | -- |
| 실시예 6Example 6 | 30 중량부30 parts by weight | -- | -- | -- | -- | 12 중량부12 parts by weight | -- |
| 실시예 7Example 7 | 30 중량부30 parts by weight | -- | -- | -- | -- | -- | 12 중량부12 parts by weight |
| 실시예 8Example 8 | 30 중량부30 parts by weight | 2중량부2 parts by weight | 2중량부2 parts by weight | 2중량부2 parts by weight | 2중량부2 parts by weight | 2중량부2 parts by weight | 2중량부2 parts by weight |
| 비교예 1Comparative Example 1 | -- | -- | -- | -- | -- | -- | -- |
| 비교예 2Comparative Example 2 | -- | 30 중량부30 parts by weight | -- | -- | -- | -- | -- |
| 비교예 3Comparative Example 3 | -- | -- | 30 중량부30 parts by weight | -- | -- | -- | -- |
| 비교예 4Comparative Example 4 | -- | -- | -- | 30 중량부30 parts by weight | -- | -- | -- |
| 비교예 5Comparative Example 5 | -- | -- | -- | -- | 30 중량부30 parts by weight | -- | -- |
| 비교예 6Comparative Example 6 | -- | -- | -- | -- | -- | 30 중량부30 parts by weight | -- |
| 비교예 7Comparative Example 7 | -- | -- | -- | -- | -- | 30 중량부30 parts by weight |
<시험예 1> 항균력 측정<Test Example 1> Measurement of antibacterial activity
(1) 대상균주(1) Target strain
Escherichia coli(KCTC 1039) 및 Staphylococcus epidermidis(KCTC 1917)의 액체배지로는 nutrient broth (NB)를 사용하였고, Staphylococcus aureus(KCTC 1621)의 액체배지로는 tryptic soy broth (TSB)를 사용하였고 Propionibacterium acnes(KCTC 3314)는 GAM을 사용하였고 고체배지는 상기 액체배지에 agar를 첨가하여 사용하였다. 균주는 BOD incubator에서 생육 온도조건별로 배양하였다.Nutrient broth (NB) was used as a liquid medium for Escherichia coli (KCTC 1039) and Staphylococcus epidermidis (KCTC 1917), and tryptic soy broth (TSB) was used as a liquid medium for Staphylococcus aureus (KCTC 1621), and Propionibacterium acnes ( KCTC 3314) used GAM, and the solid medium was used by adding agar to the liquid medium. The strains were cultured for each growth temperature condition in a BOD incubator.
(2) 감수성 검사를 위한 디스크 확산법(2) Disc diffusion method for susceptibility test
상기 실시예 1 내지 8 및 비교예 1 내지 7의 티슈에 대하여, 시험 균주로 대장균(Escherichia coli), 스타필로코커스 에피데르미스(Staphylococcus epidermidis), 스타필로코커스 아우레우스(Staphylococcus aureus) 및 프로피오니박테리움 아크네스(Propionibacterium acnes)에 대한 항균력을 측정하였다.For the tissues of Examples 1 to 8 and Comparative Examples 1 to 7, as test strains Escherichia coli , Staphylococcus epidermidis , Staphylococcus aureus , and propioni Antibacterial activity against Propionibacterium acnes was measured.
상기 시험 균주들이 도포된 고체 한천 배지에 상기 실시예 1 내지 8 및 비교예 1 내지 7의 티슈가 도포된 디스크를 올려놓고 배양한 후, 항균력을 측정하였다. 항균력 측정은 확산법(Paper Disc Method)을 이용했으며 디스크 주변의 투명환의 크기를 비교하여 항균력을 평가하여 그 결과를 하기 표 2에 나타내었다.The discs coated with the tissues of Examples 1 to 8 and Comparative Examples 1 to 7 were placed on the solid agar medium coated with the test strains and cultured, and then the antibacterial activity was measured. The antimicrobial activity was measured using a diffusion method (Paper Disc Method), and the antibacterial activity was evaluated by comparing the size of the transparent ring around the disk, and the results are shown in Table 2 below.
| Escherichia coliEscherichia coli | Staphylococcus epidermidisStaphylococcus epidermidis | Staphylococcus aureusStaphylococcus aureus | Propionibacterium acnesPropionibacterium acnes | |
| 실시예 1Example 1 | ++++ | ++++ | ++++ | ++++ |
| 실시예 2Example 2 | ++++ | ++++ | ++++ | ++++ |
| 실시예 3Example 3 | ++++ | ++++ | ++++ | ++++ |
| 실시예 4Example 4 | ++++ | ++++ | ++++ | ++++ |
| 실시예 5Example 5 | ++++ | ++++ | ++++ | ++++ |
| 실시예 6Example 6 | ++++ | ++++ | ++++ | ++++ |
| 실시예 7Example 7 | ++++ | ++++ | ++++ | ++++ |
| 실시예 8Example 8 | ++++ | ++++ | ++++ | ++++ |
| 비교예 1Comparative Example 1 | -- | -- | -- | -- |
| 비교예 2Comparative Example 2 | -+-+ | -+-+ | -+-+ | -+-+ |
| 비교예 3Comparative Example 3 | -+-+ | -+-+ | -+-+ | -+-+ |
| 비교예 4Comparative Example 4 | -+-+ | -+-+ | -+-+ | -+-+ |
| 비교예 5Comparative Example 5 | -+-+ | -+-+ | -+-+ | -+-+ |
| 비교예 6Comparative Example 6 | -+-+ | -+-+ | -+-+ | -+-+ |
| 비교예 7Comparative Example 7 | -+-+ | -+-+ | -+-+ | -+-+ |
(-: 투명환 생성 못함, -+: 투명환 생성 후 균주 성장, +: 약한 투명환 생성 ++: 강한 투명환 생성)(-: unable to create transparent ring, -+: growing strain after generating transparent ring, +: creating weak transparent ring ++: generating strong transparent ring)
상기 표 2의 결과를 참조하면, 본 발명의 실시예 1 내지 8의 티슈는 비교예 1 내지 7보다 항균성이 매우 우수함을 알 수 있었다. 특히 일반 티슈인 비교예 1의 경우에는 투명환을 전혀 생성하지 못함을 확인할 수 있었다.Referring to the results of Table 2, it was found that the tissues of Examples 1 to 8 of the present invention have very excellent antibacterial properties than Comparative Examples 1 to 7. In particular, in the case of Comparative Example 1, which is a general tissue, it was confirmed that the transparent ring was not generated at all.
<시험예 2> 피지분비 억제효능 시험<Test Example 2> Sebum secretion inhibition efficacy test
상기 실시예 1의 티슈의 피지분비 억제효과 측정을 위해 피지세포(human sebocyte, Cellprogen, USA)의 지방소립(lipid droplet) 형성 억제정도를 통해 피지 생성 저해정도를 측정하였다. 구체적으로 피지세포를 콜라겐이 코팅된 24 웰-플레이트에 2 x 103 세포/웰로 seeding하여 배양한 뒤 티슈를 도말하고 피지세포의 분화를 유도하는 인슐린 20 nM을 처리하였다. 처리 후 8일 동안 배양한 다음 세포 내 지방을 적색으로 염색하는 nile red 로 지방을 염색한 후 생성된 지질량을 형광 현미경을 통해 측정하였다. 그 결과 아무것도 처리하지 않은 음성대조군과 비교하여 인슐린 20 nM 처리군에서 지방이 염색된 세포가 눈에 띄게 증가하여 지방분화가 정상적으로 이루어졌음을 확인할 수 있었다. 티슈를 처리한 조건에서는 지방분화를 효과적으로 억제하는 결과를 관찰할 수 있었다. 그 결과를 도 1에 나타내었다.In order to measure the sebum secretion inhibitory effect of the tissue of Example 1, the degree of inhibition of sebum production was measured through the degree of inhibition of the formation of lipid droplets of sebaceous cells (human sebocyte, Cellprogen, USA). Specifically, sebum cells were seeded and cultured on a collagen-coated 24-well plate at 2 x 10 3 cells/well, and then the tissue was plated and treated with 20 nM of insulin to induce the differentiation of sebaceous cells. After incubation for 8 days after treatment, the lipids produced were measured through a fluorescence microscope after staining the fat with nile red, which stains the red fat in the cells. As a result, it could be confirmed that fat-stained cells were significantly increased in the insulin 20 nM-treated group compared to the negative control group that was not treated with anything, resulting in normal differentiation of fat. In the tissue-treated condition, the results of effectively inhibiting the differentiation of fat were observed. The results are shown in FIG. 1.
도 1은 본 발명에 따른 편백 추출물 함유 티슈 처리 후 피지분비 억제 정도를 보여주는 것이다.Figure 1 shows the degree of sebum secretion inhibition after treatment with a cypress extract-containing tissue according to the present invention.
<시험예 3> 아토피 치료 효과 확인<Test Example 3> Confirmation of atopic treatment effect
사람의 단핵구(monocyte)인 THP-1 세포(human acute monocytic leukemia cell)를 항생제(penicillinm 104 U/㎖, streptomycin 10㎎/㎖, amphotericin B 25㎍/㎖)와 FBS(fetal bovine serum)가 10%(v/v) 포함된 RPMI 1640 배지를 이용하여, 37℃, 5% CO2 조건에서 3일간 배양하였다. 이 후, MCP-1, IL-6, IL-8의 활성을 측정하기 위해 상기 THP-1 세포를 트립신-EDTA를 이용하여 2.0ㅧ106 ㎖의 농도로 24웰 플레이트에 분주한 후 FBS가 0.5%(v/v) 포함된 RPMI에서 37℃, 5% CO2 조건에서 16시간 동안 배양하였다. 다음으로, 진드기 추출물(1㎍/㎖)을 각 웰에 아토피 유발원으로 처리하고, 동시에 DMSO(dimethyl sulfoxide)로 실시예 1 내지 8 및 비교예 1 내지 7의 티슈를 14시간 동안 처리한 후 ELISA(enzyme-linked immunospecific assay ; Cytokine. 2008, 42(3), 365~371)를 이용하여 상층액에서 MCP-1, IL-6, IL-8의 양을 측정하여 표 3에 나타내었다. 이 때 각 사이토카인의 양은 진드기 추출물만을 처리한 세포에서의 값을 1.00으로 하여 대비한 fold 값을 나타내었다.Human acute monocytic leukemia cells (THP-1 cells) were treated with antibiotics (penicillinm 10 4 U/ml, streptomycin 10 mg/ml, amphotericin B 25㎍/ml) and FBS (fetal bovine serum) 10. Using RPMI 1640 medium containing% (v/v), it was cultured for 3 days at 37° C. and 5% CO 2 . Thereafter, in order to measure the activity of MCP-1, IL-6, and IL-8, the THP-1 cells were dispensed into a 24-well plate at a concentration of 2.0 x 10 6 ml using trypsin-EDTA, and the FBS was 0.5 Incubated for 16 hours at 37°C and 5% CO 2 in RPMI containing% (v/v). Next, the tick extract (1 ㎍ / ㎖) was treated as an atopic inducer in each well, and at the same time, the tissues of Examples 1 to 8 and Comparative Examples 1 to 7 were treated with DMSO (dimethyl sulfoxide) for 14 hours, and then ELISA (enzyme-linked immunospecific assay; Cytokine. 2008, 42(3), 365~371) was used to measure the amounts of MCP-1, IL-6, and IL-8 in the supernatant and shown in Table 3. At this time, the amount of each cytokine represented a fold value compared to the value in cells treated with only the tick extract as 1.00.
| MCP-1MCP-1 | IL-6IL-6 | IL-8IL-8 | |
| 진드기 추출물Tick extract | 1.001.00 | 1.001.00 | 1.001.00 |
| 실시예 1Example 1 | 0.210.21 | 0.220.22 | 0.230.23 |
| 실시예 2Example 2 | 0.170.17 | 0.200.20 | 0.190.19 |
| 실시예 3Example 3 | 0.200.20 | 0.180.18 | 0.170.17 |
| 실시예 4Example 4 | 0.190.19 | 0.170.17 | 0.160.16 |
| 실시예 5Example 5 | 0.170.17 | 0.180.18 | 0.190.19 |
| 실시예 6Example 6 | 0.170.17 | 0.160.16 | 0.170.17 |
| 실시예 7Example 7 | 0.190.19 | 0.180.18 | 0.170.17 |
| 실시예 8Example 8 | 0.110.11 | 0.100.10 | 0.110.11 |
| 비교예 1Comparative Example 1 | 0.930.93 | 0.920.92 | 0.930.93 |
| 비교예 2Comparative Example 2 | 0.820.82 | 0.820.82 | 0.830.83 |
| 비교예 3Comparative Example 3 | 0.840.84 | 0.830.83 | 0.840.84 |
| 비교예 4Comparative Example 4 | 0.860.86 | 0.850.85 | 0.790.79 |
| 비교예 5Comparative Example 5 | 0.870.87 | 0.860.86 | 0.810.81 |
| 비교예 6Comparative Example 6 | 0.850.85 | 0.840.84 | 0.910.91 |
| 비교예 7Comparative Example 7 | 0.850.85 | 0.850.85 | 0.830.83 |
상기 표 3에 개시된 바와 같이 THP-1 세포에 아토피를 유발한다고 알려진 진드기 추출물을 처리하면 MCP-1, IL-6 및 IL-8의 분비가 증가됨을 알 수 있다. 본 발명의 편백 추출물을 포함하는 티슈를 처리한 세포에서는 MCP-1, IL-6 및 IL-8의 분비가 현저하게 감소되는 것으로 확인되어, 상기 조성물이 아토피 피부염의 치료 효과가 우수함을 알 수 있다.As disclosed in Table 3 above, it can be seen that the secretion of MCP-1, IL-6 and IL-8 is increased when the mite extract known to induce atopy in THP-1 cells is treated. It was confirmed that the secretion of MCP-1, IL-6 and IL-8 was remarkably reduced in the cells treated with the tissue containing the cypress extract of the present invention, and it can be seen that the composition has an excellent therapeutic effect on atopic dermatitis .
<시험예 4> 여성질염 균주에 항균효과 측정<Test Example 4> Measurement of antibacterial effect on female vaginitis strain
상기 실시예 1 내지 8 및 비교예 1 내지 7에서 제조된 티슈에 대하여 여성질염 균주에 대한 항균활성을 확인하기 위하여 디스크 확산법을 이용하였다.In order to confirm the antimicrobial activity against the female vaginitis strain with respect to the tissues prepared in Examples 1 to 8 and Comparative Examples 1 to 7, a disk diffusion method was used.
1. 칸디다알비칸스에 대한 항균력 평가1. Evaluation of antibacterial activity against Candida albicans
전 배양된 칸디다 알비칸스 (Candida albicans)를 디스크 확산법 측정용 평판배지(Sabouraud [0057] Dextrose Agar. Difco.USA)에 100 ㎕씩 분주하여 도말한 후, 티슈를 멸균된 페이퍼 디스크에 올려놓았다. 이에 대한 대조군으로 항진균제인 이트라코나졸 (Itraconazol)을 페이퍼디스크에 100㎍/disk의 농도로 시료를 점적한 후 배지 위에 올려놓았다. 이 평판배지를 28℃에서 48시간 동안 배양한 후 생육저지환(Clean Zone)의 크기를 측정하였다. 그 결과를 하기 표 4에 나타내었다. Pre-cultured Candida albicans (Candida albicans) was dispensed into a plate medium for measuring the disk diffusion method (Sabouraud Dextrose Agar. Difco.USA) by 100 µl and smeared, and then the tissue was placed on a sterilized paper disk. As a control for this, an antifungal agent, Itraconazol, was dropped onto a paper disk at a concentration of 100 µg/disk and then placed on a medium. After culturing the plate medium at 28° C. for 48 hours, the size of the growth inhibition ring (Clean Zone) was measured. The results are shown in Table 4 below.
하기 표 4에서 보는 바와 같이 본 발명의 실시예 1 내지 8의 편백 추출물 함유 티슈는 대조군인 이트라코나졸뿐만 아니라 비교예 1 내지 7의 티슈에 비해 강한 생육저지효과를 나타내었다.As shown in Table 4 below, the tissues containing cypress extracts of Examples 1 to 8 of the present invention exhibited a strong growth inhibition effect compared to the tissues of Comparative Examples 1 to 7 as well as itraconazole as a control.
2. 가드네렐라 바지날리스에 대한 항균력 평가2. Evaluation of antibacterial activity against Gardnerella vaginalis
전 배양된 가드네렐라 바지날리스 (Gardnerella vaginalis)를 디스크 확산법 측정용 평판배지(Reinforced clostridial medium. Difco.USA)에 100 ㎕씩 분주하여 도말한 후, 티슈를 멸균된 페이퍼 디스크에 올려놓았다. 이에 대한 대조군으로 항생제인 앰피실린(Ampicillin)을 페이퍼디스크에 10㎍/disk의 농도로 시료를 점적한 후 배지 위에 올려놓았다. 이 평판배지를 37℃에서 48시간The pre-cultured Gardnerella vaginalis was dispensed into a plate medium for disc diffusion measurement (Reinforced clostridial medium. Difco.USA) by 100 µl and spread, and then the tissue was placed on a sterilized paper disc. As a control for this, the antibiotic Ampicillin was dropped onto a paper disk at a concentration of 10 µg/disk, and then placed on the medium. This plate medium was placed at 37℃ for 48 hours.
동안 배양한 후 생육저지환(Clean Zone)의 크기를 측정하였다. 그 결과를 하기 표 4에 나타내었다. 하기 표 4에서 보는 바와 같이 본 발명의 편백 추출물 함유 티슈가 강한 생육저지효과를 나타내었다.After culturing during, the size of the growth inhibition ring (Clean Zone) was measured. The results are shown in Table 4 below. As shown in Table 4 below, the tissue containing cypress extract of the present invention exhibited a strong growth inhibition effect.
| 칸디다 알비칸스Candida albicans | 가드네렐라 바지날리스Gardnerella Vaginalis | |
| 대조군Control | 3030 | 3131 |
| 실시예 1Example 1 | 2424 | 2323 |
| 실시예 2Example 2 | 2727 | 2626 |
| 실시예 3Example 3 | 2626 | 2626 |
| 실시예 4Example 4 | 2525 | 2626 |
| 실시예 5Example 5 | 2727 | 2525 |
| 실시예 6Example 6 | 2626 | 2525 |
| 실시예 7Example 7 | 2626 | 2626 |
| 실시예 8Example 8 | 3232 | 3232 |
| 비교예 1Comparative Example 1 | -- | -- |
| 비교예 2Comparative Example 2 | 1One | 1One |
| 비교예 3Comparative Example 3 | 22 | 22 |
| 비교예 4Comparative Example 4 | 22 | 22 |
| 비교예 5Comparative Example 5 | 22 | 1One |
| 비교예 6Comparative Example 6 | 1One | 22 |
| 비교예 7Comparative Example 7 | 22 | 22 |
<시험예 5> 티슈의 피부자극 시험피험자 선정 기준에 부합하며, 신체가 건강하고 20 ~ 40세 사이의 건강한 성인 10명(남성 5명, 여성 5명)을 대상으로 피부 자극 실험을 실시하였으며, 시험대상자의 상박부를 70% 알코올로 세척하고 실시예 1의 티슈를 폐쇄첩포하였다. <Test Example 5> Skin irritation test of tissue A skin irritation experiment was conducted on 10 healthy adults (5 males and 5 females) that meet the criteria for selecting subjects, have a healthy body and are between the ages of 20 and 40, The upper arm of the test subject was washed with 70% alcohol, and the tissue of Example 1 was closed.
1. 적용부위 : Inner arm1. Application part: Inner arm
2. 시험 방법 : 패치를 적용부위에 24시간/48시간 동안 첩포하였다가 제거한 후 20분 후 피부 자극 정도를 평가하였다.2. Test method: The patch was applied on the applied area for 24 hours/48 hours, removed, and the skin irritation level was evaluated after 20 minutes.
3. 판정 기준 : 첩포 제거 후 육안 평가하여 아래의 표 5의 기준에 따라 점수를 부여하였다.3. Judgment Criteria: After removing the patch, it was evaluated visually and scored according to the criteria in Table 5 below.
| 등급rank | 자극 정도Degree of stimulation |
| 00 | No ReactionNo Reaction |
| 1One | Slight ErythemaSlight Erythema |
| 22 | Strong ErythemaStrong Erythema |
| 33 | EdemaEdema |
| NoNo | 나이age | 성별gender | 피부타입Skin type | 24시간24 hours | 48시간48 hours | 결과result |
| 1One | 2222 | 남male | normalnormal | 00 | 00 | 양호Good |
| 22 | 2121 | 남male | DryDry | 00 | 00 | 양호Good |
| 33 | 2222 | 남male | normalnormal | 00 | 00 | 양호Good |
| 44 | 2323 | 남male | oliyoliy | 00 | 00 | 양호Good |
| 55 | 2424 | 남male | normalnormal | 00 | 00 | 양호Good |
| 66 | 2424 | 여female | normalnormal | 00 | 00 | 양호Good |
| 77 | 2929 | 여female | DryDry | 00 | 00 | 양호Good |
| 88 | 3535 | 여female | normalnormal | 00 | 00 | 양호Good |
| 99 | 3636 | 여female | normalnormal | 00 | 00 | 양호Good |
| 1010 | 4444 | 여female | sensitivesensitive | 00 | 00 | 양호Good |
상기 표 6과 같이 본 발명에 따른 티슈로 실험을 하였을 때 피부 안전성 측면에서 피부에 자극을 주지 않으면서 짖무름이나 가려움이 동반되지 않음을 알 수 있다.As shown in Table 6, when the experiment was conducted with the tissue according to the present invention, in terms of skin safety, it can be seen that bark or itching is not accompanied without irritation to the skin.
<시험예 6> 관능 평가<Test Example 6> Sensory evaluation
상기 실시예 1 및 8 및 비교예 1 및 2에서 제조된 티슈에 대하여, 20~60대 여성 40명을 대상으로, 질염 감염부위에 30일 동안 사용하게 한 다음 불쾌한 냄새, 사용감 및 전체적인 기호도를 5점 만점으로 평가하여 그 결과를 하기 표 7에 나타내었다.For the tissues prepared in Examples 1 and 8 and Comparative Examples 1 and 2, 40 women in their 20s to 60s were allowed to use it for 30 days at the site of vaginitis infection, and then the unpleasant odor, feeling of use, and overall acceptability were measured. It evaluated with a perfect score and the results are shown in Table 7 below.
| 불쾌한 냄새Unpleasant smell | 사용감Feeling | 전체적인 기호도Overall preference | |
| 실시예 1Example 1 | 55 | 55 | 55 |
| 실시예 8Example 8 | 55 | 55 | 55 |
| 비교예 1Comparative Example 1 | 1One | 1One | 1One |
| 비교예 2Comparative Example 2 | 22 | 22 | 22 |
상기 표 7의 결과를 참조하면, 본 발명에 따른 편백 추출물 함유 티슈를 여성 질염 감염 부위에 사용할 불쾌한 냄새가 완전히 제거되어 전체적인 기호도가 매우 높은 것을 알 수 있었다. Referring to the results of Table 7 above, it was found that the unpleasant odor to be used for the female vaginitis infected site of the tissue containing the cypress extract according to the present invention was completely removed, and the overall preference was very high.
본 발명은 편백 추출물을 함유하는 항아토피 및 항질염용 티슈 및 이의 제조방법에 관한 산업에 이용될 수 있다.The present invention can be used in the industry related to the anti-atopy and anti-vaginitis tissue containing a cypress extract and a method for producing the same.
Claims (5)
- 티슈 원단 100 중량부에 대하여, 편백나무의 잎을 2 내지 5배 양(w/v)의 증류수를 넣고 1%(v/v)의 소금을 넣어 90℃~110℃에서 1 내지 2시간 동안 추출한 후 여과하여 수득된 편백 추출물 1 내지 40 중량부를 포함하며,With respect to 100 parts by weight of tissue fabric, 2 to 5 times the amount (w/v) of distilled water was added to the leaves of cypress, and 1% (v/v) of salt was added to extract for 1 to 2 hours at 90°C to 110°C. It contains 1 to 40 parts by weight of the cypress extract obtained by filtration after,쑥 추출물, 모링가 추출물, 대나무 추출물, 병풀 추출물, 소나무 추출물 및 질경이 추출물을 티슈 원단 100 중량부에 대하여 1 내지 20 중량부의 양으로 포함하고,Mugwort extract, moringa extract, bamboo extract, centella extract, pine extract and plantain extract in an amount of 1 to 20 parts by weight based on 100 parts by weight of tissue fabric,쑥 추출물, 모링가 추출물, 대나무 추출물, 병풀 추출물, 소나무 추출물 및 질경이 추출물을 1:1:1:1:1:1 중량비로 혼합하여 첨가하는 것을 특징으로 하는 항아토피 및 항질염용 티슈.Mugwort extract, moringa extract, bamboo extract, centella asiatica extract, pine extract and plantain extract are added by mixing in a weight ratio of 1:1:1:1:1:1. Anti-atopy and anti-vaginitis tissue.
- 제 1 항에 있어서,The method of claim 1,상기 티슈가 피부 짖무름 또는 가려움을 일으키지 않는 것을 특징으로 하는 항아토피 및 항질염용 티슈.Anti-atopy and anti-vaginitis tissue, characterized in that the tissue does not cause barking or itching of the skin.
- 제 1 항에 있어서,The method of claim 1,상기 티슈는 건티슈 또는 물티슈인 것을 특징으로 하는 항아토피 및 항질염용 티슈.The tissue is an anti-atopy and anti-vaginitis tissue, characterized in that the dry tissue or wet tissue.
- 제 1 항에 있어서,The method of claim 1,상기 질염이 칸디다성 질염 또는 세균성 질염인 것을 특징으로 하는 항아토피 및 항질염용 티슈.Anti-atopy and anti-vaginitis tissue, characterized in that the vaginitis is Candida vaginitis or bacterial vaginosis.
- 편백나무의 잎을 2 내지 5배 양(w/v)의 증류수를 넣고 1%(v/v)의 소금을 넣어 90℃~110℃에서 1 내지 2시간 동안 추출한 후 여과하여 편백 추출물을 수득하고,The leaves of the cypress tree were extracted with 2 to 5 times the amount (w/v) of distilled water and 1% (v/v) of salt for 1 to 2 hours at 90°C to 110°C, and then filtered to obtain a cypress extract. ,티슈 원단 100 중량부에 대하여 편백 추출물을 1 내지 40 중량부의 양으로 혼합하며,Cypress extract is mixed in an amount of 1 to 40 parts by weight based on 100 parts by weight of the tissue fabric,쑥 추출물, 모링가 추출물, 대나무 추출물, 병풀 추출물, 소나무 추출물 및 질경이 추출물을 티슈 원단 100 중량부에 대하여 1 내지 20 중량부의 양으로 포함하고,Mugwort extract, moringa extract, bamboo extract, centella extract, pine extract and plantain extract in an amount of 1 to 20 parts by weight based on 100 parts by weight of tissue fabric,쑥 추출물, 모링가 추출물, 대나무 추출물, 병풀 추출물, 소나무 추출물 및 질경이 추출물을 1:1:1:1:1:1 중량비로 혼합하여 첨가하는 것을 특징으로 하는 항아토피 및 항질염용 티슈의 제조방법.Mugwort extract, Moringa extract, bamboo extract, Centella asiatica extract, pine extract and plantain extract are mixed in a 1:1:1:1:1:1 weight ratio and added to prepare a tissue for anti-atopy and anti-vaginitis .
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| KR102271989B1 (en) | 2020-09-09 | 2021-07-02 | 주식회사 와일드원 | Preparation method of chamaecyparis obtusa essential oil extract and antimicrobial composition comprising thereof |
| KR102501800B1 (en) * | 2021-01-05 | 2023-02-22 | (주)엔비바이오컴퍼니 | A composition with anti-inflammation activity and skin elasticity improvement efficacy comprising mixed ferment extracts of natural products |
| KR102629656B1 (en) * | 2021-01-28 | 2024-01-29 | 계명대학교 산학협력단 | Natural preservative composition comprising Chamaecyparis obtusa extract, Grapefruit seed extract and Germinated brown rice extract |
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| KR20120041416A (en) * | 2010-10-21 | 2012-05-02 | 전라남도 | Development method of skin disease enhancer from japanese cypress and bittern |
| KR20120098117A (en) * | 2011-02-28 | 2012-09-05 | 우인창 | Wet tissue comprising phytoncide essential oil and method for manufacturing the same |
| KR101298423B1 (en) * | 2011-12-27 | 2013-08-26 | 강현숙 | Antibacterial and deodorant functional wet tissue with Chamaecyparis sp. essential oil without using any chemical additives |
| KR20160015718A (en) * | 2014-07-31 | 2016-02-15 | 이은선 | Feminine cleanser composition comprising Portulaca oleracea extract and Phytoncide |
| KR20190017585A (en) * | 2017-08-11 | 2019-02-20 | (주) 피러스 | functional hygienic goods and their preparation method |
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| KR20120041416A (en) * | 2010-10-21 | 2012-05-02 | 전라남도 | Development method of skin disease enhancer from japanese cypress and bittern |
| KR20120098117A (en) * | 2011-02-28 | 2012-09-05 | 우인창 | Wet tissue comprising phytoncide essential oil and method for manufacturing the same |
| KR101298423B1 (en) * | 2011-12-27 | 2013-08-26 | 강현숙 | Antibacterial and deodorant functional wet tissue with Chamaecyparis sp. essential oil without using any chemical additives |
| KR20160015718A (en) * | 2014-07-31 | 2016-02-15 | 이은선 | Feminine cleanser composition comprising Portulaca oleracea extract and Phytoncide |
| KR20190017585A (en) * | 2017-08-11 | 2019-02-20 | (주) 피러스 | functional hygienic goods and their preparation method |
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