CN104094923A - Preservative liquid for corpses, tissue and dissections, and application method thereof - Google Patents
Preservative liquid for corpses, tissue and dissections, and application method thereof Download PDFInfo
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- CN104094923A CN104094923A CN201310119539.4A CN201310119539A CN104094923A CN 104094923 A CN104094923 A CN 104094923A CN 201310119539 A CN201310119539 A CN 201310119539A CN 104094923 A CN104094923 A CN 104094923A
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Abstract
The invention provides a preservative liquid for corpses, tissue and dissections and an application method thereof. The preservative liquid mainly comprises 0.5 to 2% by mass of glutaraldehyde, 0.3 to 3% by mass of thymol, 0.5 to 3% by mass of sodium benzoate, 0.1 to 1% by mass of sodium citrate (or citric acid), 0.5 to 3% by mass of mannitol (or D-sorbitol), 40 to 65% by volume of ethanol, 5 to 15% by volume of glycerol, 1 to 5% by volume of organic acid, 0 to 1% by volume of essence and 14 to 54% by volume of water. The preservative liquid can realize preservation and fixation of corpses, tissue and dissections via a soaking or perfusion method and exerts anticorrosive, preservative, disinfecting and stink-removing effects. According to results of animal experiments of the preservative liquid in a laboratory, on-site experiments in a mortuary house, etc., the preservative liquid has high efficiency bacteriostatic performance, safety and low toxicity and is applicable to preservation of corpses, tissue and dissections in medical teaching and scientific research and funeral services.
Description
Technical field
The present invention relates to the embalming Techniques of preserving in human dissection teaching and scientific research field and field of funeral and interment, be specifically related to a kind of antiseptic preserving fluid for corpse, tissue and dissection and using method thereof.
Background technology
Understanding organization of human body, seek the demand of Know Your Body and modern funeral service, be unable to do without autopsy and histiocytic research, require to have the anticorrosion and Techniques of preserving for corpse, tissue and organ of a set of science.Human or animal after death, if corpse without anticorrosion fixing processing, will soon occur tissue automatic soup-dissolving, rot.Civil administration funeral service unit, in the time carrying out the business such as international fortune corpse, remains preservation, also often uses Gentle Repose technology.
Conventional embalming store method has: medicine is anticorrosion, vacuum or inert gas is anticorrosion, dry anticorrosion, ultralow temperature preservation etc.For fully keeping the shape characteristic of corpse and tissue, no matter at medical domain or in funeral and interment department, generally use medicine (being generally formaldehyde) anti-corrosion method both at home and abroad.But formaldehyde and antiseptic preservation method have organize hard and crisp, elasticity is little, anti-mold effect is poor, and has strong impulse smell, and human body is had to the serious harm such as sensitization, mutagenesis.
For reducing formaldehyde to environment and healthy harm, researcher has proposed a lot of improvement methods, as the concentration, the inhibition formaldehyde that reduce formaldehyde volatilize, eliminate formaldehyde odor, use (the bibliography: Yang Dehui such as formaldehyde substitute, Deng. the anticorrosion experiment effect of a kind of isothiazolinone compound preservative cavy is observed, Chinese Clinical Chinese Journal of anatomy, 2011,29 (4): 103-106).
Than formaldehyde, it is larger that glutaraldehyde (Glutaraldehyde) has molecule, and not volatile, penetrating odor is little, and glutaraldehyde reacts also be not as strong like that as formaldehyde with protein.Glutaraldehyde belongs to high effect disinfectants, have wide spectrum, efficient, low toxicity, little to metal protection, be subject to the features such as organic substance influence is little, good stability.Glutaraldehyde and albumino reaction have a wider pH value scope, this for anticorrosion be a very important advantage.Because of be people after death, from death to anticorrosion during this period of time, the pH value of the different parts of body is different.Facts have proved, glutaraldehyde is better than formaldehyde as preservative, but its cost ratio formaldehyde costliness.Answer rational allocation antiseptic preserving fluid component, reduce formulation cost.Zhao Duo etc. disclose cadaver-preservative fluid and preparation method thereof (number of patent application 200510119111.5), are characterized in being made up of 50% glutaraldehyde, the rare fatty alcohol ether of polyoxy second, ethanol, benzalkonium bromide, strong disinfecting preservative, the hundred mould fungusproof anti-corrosion agents, glycerine, water of killing.
The desirable antiseptic preserving fluid for corpse, tissue and dissection, should have low toxicity, excitant little, use that simple, cheap, anticorrosion internal validity sample shrinkage factor strong, that preserve is little, color and luster approaches the features such as condition of living organism.For overcoming the existing shortcoming taking formaldehyde, peroxide as main remains (corpse) preservative, further improve the performance of existing antiseptic preserving fluid, adapt to the anticorrosion preservation requirement of various environment and region, in the urgent need to developing the antiseptic preserving fluid for corpse, tissue and dissection of a kind of efficient, low toxicity, environmental protection, by methods such as infusion method or remains arterial perfusion, body cavity suction injections, effectively prevent the propagation of growing of the interior pathogenic microorganism of remains, control the development of remains self-dissolving, corruption, be beneficial to medical anatomy scientific research or funeral parlor Gentle Repose and preserve.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, provide a kind of antiseptic preserving fluid and using method thereof of efficient, the environmental protection for corpse, tissue and dissection, to be applied to the anticorrosion preservation of funeral service and corpse medically, tissue and dissection.
The object of the present invention is achieved like this:
A kind of antiseptic preserving fluid for corpse, tissue and dissection provided by the invention and using method thereof, is characterized in that:
The ethanol that the glutaraldehyde that antiseptic preserving fluid of the present invention is mainly 0.5～2% by mass percent, 0.3～3% thymol, 0.5～3% Sodium Benzoate, 0.1～1% sodium citrate (or citric acid), 0.5～3% mannitol (or D-glucitol) and volume ratio are 40～65%, 5～15% glycerine, 1～5% organic acid, 0～1% essence, 14～54% water form.
Antiseptic preserving fluid of the present invention, containing formaldehyde, inorganic oxide and organic oxygen compound; The preferred acetic acid of organic acid and propionic acid, the flavoring essences such as the preferred pineapple of essence, orange.
The preparation method of antiseptic preserving fluid of the present invention comprises the steps: by recipe ratio, and Sodium Benzoate is soluble in water, adds successively sodium citrate (or citric acid), mannitol (or D-glucitol), stirs, and fully dissolves; Then add while stirring ethanol, glutaraldehyde, thymol, glycerine, organic acid and essence, mix and get final product.
Antiseptic preserving fluid of the present invention can adopt infusion method or perfusion to fix corpse, tissue and dissection are anticorrosion, inject preservative by the perfusion of sustainer, the arch of aorta or abdomen/chest/pharynx/cranial cavity, play remains effectively anticorrosion, preservation, sterilization, the smelly effect of Eradicates rapidly.
Antiseptic preserving fluid provided by the invention and using method thereof, have following advantage:
This preservative is not containing formaldehyde, inorganic oxide and organic oxygen compound, and excitant is little, little to environment and human health risk.Its preparation method is easy, and raw material is easy to get, workable; Practical application is convenient, is beneficial to and promotes the use of.
The present invention is compound antiseptic preserving fluid, contains preservative, disinfectant, anticoagulant, bleeding agent, humectant etc. in its component, and rationally, ratio is suitable for formula, and each component plays synergy.
While utilizing antiseptic preserving fluid of the present invention to process remains, can discharge remains inner blood, or not bloodletting, should be decided according to the actual requirements.The anti-corrosion method of standard is otch, bloodletting in the world; If do not discharge remains inner blood, when operation, ooze out without corpse liquid, free from environmental pollution, funeral belongs to more easily to be accepted.
Antiseptic preserving fluid of the present invention, has efficient biocidal property, and safety and low toxicity are applicable to the anticorrosion preservation of corpse, tissue and dissection in medical teaching scientific research and funeral services.
Brief description of the drawings
Fig. 1 is: each histologic section microscope figure (200 ×) after medical college's embalming
(A) cardiac muscle, (B) liver, (C) spleen, (D) lungs, (E) kidney, (F) coat of the stomach, (G) small intestine, (H) large intestine, (I) skin.
Embodiment
Embodiment 1: soak anticorrosion preservation experiment
In specimen bottle, by after each mouse peritoneal injection 10mL antiseptic preserving fluid of the present invention, be soaked in this preservative, observe mouse antiseptic effect.With Spain Complucad preservative in contrast, using sterile saline as blank.Laboratory mice is purchased from Military Medical Science Institute's Experimental Animal Center, male, strain: KM, body weight 29～34g.
Mouse is soaked anticorrosion preservation 12 months (2010～2011), uses the mouse color and luster of antiseptic preserving fluid of the present invention normal, and good springiness is apparent without corruption, without fungus growth, preserves slightly Huang, clear of liquid; Use the mouse health of Complucad preservative transparent, have serious atrophy phenomenon; Mouse blackening when physiological saline control group the 7th day is observed, mouldy, there is strong odor, limbs are broken gradually, then abandon in the 10th day.Experimental result shows: antiseptic preserving fluid of the present invention is significantly better than Complucad preservative.
Embodiment 2: laboratory rat is injected anticorrosion experiment
After three groups of experimental rats are put to death, by the dosage injection of heart preservative of 25mL/100g, be placed in the dry place of shady and cool ventilation, under room temperature state, deposit 30 days, observe antiseptic effect.In experiment the 3rd day, 7 days, 14 days, 30 days time, respectively get a rat and dissects and draw materials, cut respectively the tissues such as skeletal muscle, intestines, the heart, lung, liver, kidney, brain, fixing, paraffin section, hematoxylin eosin staining, microscopic examination photograph.This experiment is taking FineFIX preservative as contrast (FineFIX be Italian Milestone patent carry out the novel without formalin fixative of morphology research), using sterile saline as blank.Experimental rat is the Wistar rat of clean level, purchased from Military Medical Science Institute's Experimental Animal Center, male, body weight 150g left and right.
Experimental result is as follows: overall appearance aspect, and the 3rd day when dissected of physiological saline control group has been shown in stink, to the 7th day skin blackout, suppurates easily broken, rots serious, has foul odour; And the mouse of use preservative of the present invention and FineFIX preservative, during to 30 days, all there is not stink, preservative mouse color and luster of the present invention more approaches condition of living organism, there is the phenomenon of leakage in FineFIX mouse part, by outward appearance and anatomic observations such as mouse hardness, colors, preservative of the present invention is significantly better than FineFIX preservative.The microscopic examination of mouse tissue form pathological section, the each institutional framework of mouse is roughly normal, and the clear or small part core of cell nucleus disappears, and interstitial structure is clear, illustrates that antiseptic preserving fluid of the present invention is also better than FineFIX.From table 1, use the mouse lung liver cell nuclear quantity of antiseptic preserving fluid of the present invention significantly more than FineFIX group.
Table 1 mouse lung, liver cell nuclear quantity statistics result
| Group | Lung | Liver |
| Antiseptic preserving fluid of the present invention | 9.3±4.5(17.0-3.0) | 44.2±65.9(175.3-0) |
| FineFIX | 6.0±5.1(11.3-0) | 65.3±66.5(160.6-0) |
| Blank | 2.1±3.1(7.0-0) | 0±0(0-0) |
Embodiment 3: medical college's Gentle Repose experiment
Choose tool adult male remains (the about 60Kg of body weight) in certain medical university, the about 25000mL of arterial perfusion and splanchnocoel injection antiseptic preserving fluid of the present invention, is positioned in the environment of 4 DEG C the anticorrosion experimental result of close observation.After 50 days, observe remains without obviously corruption, odorless from outward appearance, core respectively, liver, spleen, lung, kidney, stomach, large intestine, small intestine and skin, with section after paraffin parcel, HE dyeing, observes by high power lens.As seen from the figure, use antiseptic preserving fluid of the present invention substantially can keep intrinsic form and the structure of the each cell of human body, tissue.
Embodiment 4: funeral parlor Gentle Repose field experiment
Corrosion protection preservative fluid for reliquiae of the present invention is applied 30 many cases in the south China funeral parlor, pour into and be aided with the about 2500mL of abdomen/chest/pharyngeal cavity injection antiseptic preserving fluid by femoral artery, arteria brachialis, to remains carry out disinfection, anticorrosion and preserve.Under room temperature, deposit efficient reaching more than 90% in 5 days in summer, can make remains face keep sleep shape, without decortication, chest belly dyes and swelling without green, odorless.
Claims (5)
1. for an antiseptic preserving fluid for corpse, tissue and dissection, it is characterized in that the ethanol that glutaraldehyde that antiseptic preserving fluid is mainly 0.5～2% by mass percent, 0.3～3% thymol, 0.5～3% Sodium Benzoate, 0.1～1% sodium citrate (or citric acid), 0.5～3% mannitol (or D-glucitol) and volume ratio are 40～65%, 5～15% glycerine, 1～5% organic acid, 0～1% essence, 14～54% water form.
2. antiseptic preserving fluid according to claim 1, is characterized in that not containing formaldehyde, inorganic oxide and organic oxygen compound; The preferred acetic acid of organic acid and propionic acid, the flavoring essences such as the preferred pineapple of essence, orange.
3. antiseptic preserving fluid according to claim 1, is characterized in that the preparation method of this antiseptic preserving fluid comprises the steps:
By recipe ratio, Sodium Benzoate is soluble in water, add successively sodium citrate (or citric acid), mannitol (or D-glucitol), stir, fully dissolve; Then add while stirring ethanol, glutaraldehyde, thymol, glycerine, organic acid and essence, mix and get final product.
4. the using method of antiseptic preserving fluid according to claim 1, is characterized in that adopting infusion method, arterial perfusion method or body cavity injection to carry out corpse, tissue and dissection anticorrosion fixing, plays anticorrosion, preservation, sterilization, the smelly effect of Eradicates.
5. antiseptic preserving fluid according to claim 1, has efficient biocidal property, and safety and low toxicity are applicable to the anticorrosion preservation of corpse, tissue and dissection in medical teaching scientific research and funeral services.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105519520A (en) * | 2016-01-21 | 2016-04-27 | 中国人民解放军第四军医大学 | Tissue specimen preserving fluid |
| CN105532637A (en) * | 2016-01-07 | 2016-05-04 | 别永信 | Human body teaching specimen preserving fluid and preparing method thereof |
| CN106359367A (en) * | 2016-08-30 | 2017-02-01 | 河南新大阳生物技术有限公司 | Formaldehyde-free specimen tissue preserving fluid and preparation method thereof |
| CN107183004A (en) * | 2017-06-21 | 2017-09-22 | 上海于泽生物科技有限公司 | A kind of dissec antiseptic preserving fluid |
| CN107568200A (en) * | 2017-09-29 | 2018-01-12 | 肇庆医学高等专科学校 | A kind of store method of anatomy sample |
| CN107593684A (en) * | 2017-09-29 | 2018-01-19 | 肇庆医学高等专科学校 | A kind of specimen preserving liquid and preparation method thereof |
| CN108931408A (en) * | 2018-06-15 | 2018-12-04 | 山东聚众数字医学科技开发有限公司 | A kind of anatomic teaching sample guarantor's color fixing liquid and preparation method thereof |
| CN110169417A (en) * | 2019-04-30 | 2019-08-27 | 黄恩南 | Deoderizing Gentle Repose liquid |
| CN110771596A (en) * | 2019-11-21 | 2020-02-11 | 南通大学 | Preservation method for fresh corpses |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532637A (en) * | 2016-01-07 | 2016-05-04 | 别永信 | Human body teaching specimen preserving fluid and preparing method thereof |
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| CN106359367A (en) * | 2016-08-30 | 2017-02-01 | 河南新大阳生物技术有限公司 | Formaldehyde-free specimen tissue preserving fluid and preparation method thereof |
| CN107183004A (en) * | 2017-06-21 | 2017-09-22 | 上海于泽生物科技有限公司 | A kind of dissec antiseptic preserving fluid |
| CN107568200A (en) * | 2017-09-29 | 2018-01-12 | 肇庆医学高等专科学校 | A kind of store method of anatomy sample |
| CN107593684A (en) * | 2017-09-29 | 2018-01-19 | 肇庆医学高等专科学校 | A kind of specimen preserving liquid and preparation method thereof |
| CN108931408A (en) * | 2018-06-15 | 2018-12-04 | 山东聚众数字医学科技开发有限公司 | A kind of anatomic teaching sample guarantor's color fixing liquid and preparation method thereof |
| CN108931408B (en) * | 2018-06-15 | 2021-08-27 | 山东聚众数字医学科技开发有限公司 | Color-preserving fixing liquid for dissecting teaching specimen and preparation method thereof |
| CN110169417A (en) * | 2019-04-30 | 2019-08-27 | 黄恩南 | Deoderizing Gentle Repose liquid |
| CN110771596A (en) * | 2019-11-21 | 2020-02-11 | 南通大学 | Preservation method for fresh corpses |
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