CN102461496A - Low-toxicity, environment-friendly short-term remains preservative and usage thereof - Google Patents
Low-toxicity, environment-friendly short-term remains preservative and usage thereof Download PDFInfo
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Abstract
The invention relates to a low-toxicity, environment-friendly short-term remains preservative based on alcohols and usage thereof. The remains preservative mainly comprises 60 to 75 percent of ethanol, 5 to 20 percent of propylene glycol, 3 to 15 percent of organic acid, 0 to 0.3 percent of essence and 10 to 40 percent of distilled water according to volumetric proportion as well as 0.2 to 10 percent of nipagin ester, 0.3 to 3 percent of thymol, 0.5 to 3 percent of mannitol (or sorbierite) and 0.05 to 0.5 percent of low-toxicity metal salt according to weight ratio. The invention can adopt the immersion method or the infusion method to preserve and fix remains (tissues), and the preservative can be infused via the aorta (arch) and injected via the thoracic cavity and the abdominal cavity, and has the fast and effective effects of disinfection, embalmment and preservation. The preservative is highly antibacterial, can effectively prevent bacteria from corroding the remains and the autolysis of the tissues, can be used as histocyte fixative, and is applicable to short-term remains embalmment or specimen preservation in domestic and foreign funeral homes and medical academies.
Description
Technical field
The present invention relates to the Gentle Repose preservation technology in field of funeral and interment and the human dissection teaching and scientific research field, be specifically related to a kind of agent of short-term Gentle Repose and method for using thereof of low toxic and environment-friendly.
Background technology
The human or animal after death, if tissue automatic soup-dissolving will soon take place without anticorrosion fixedly processing in corpse, corruption is disintegrated gradually.For the corrosion that prevents bacterium and the self-dissolving of tissue, must carry out anticorrosion fixedly processing to remains (corpse), tissue and dissection.Gentle Repose is meant that methods such as in a period of time, adopting physics, chemistry prevents or slows down further developing of the corrupt process of rotting of remains; Make remains be kept at relative good state and also can prevent contingent disease propagation, so that carry out funeral activities or anatomic teaching scientific research.
At present, anticorrosion fixed drug commonly used mainly contains: formaldehyde, ethanol, glycerine, phenol etc., and its anticorrosion fixing basic principle comprises: protein denaturation is solidified; Disturb the important enzyme system of microorganism; Destroy its permeability of bacterial cell membrane change etc.Because the antiseptic property and the method for every kind of medicament have nothing in common with each other, and its pluses and minuses are respectively arranged, and in order to strengthen its effect, remedy its defective, often with the multiple medications mixed preparing, to obtain desirable effect.The existing summary of remains (corpse) preservative present Research deliver (list of references 1: Zhou Xuemei, etc. dissect scientific advance, 2008,14:457-460).Generally adopt the main component of formalin (being formalin) both at home and abroad, like Chinese patent CN200810125376.X and U.S. Dodge series preservative etc. as the Gentle Repose agent prescription.But the fixed with formaldehyde and antiseptic method exist organize hard and crisp, elasticity is little; Anti-mold effect is poor; And have strong impulse property smell, human body is had serious harm (list of references 2:Naya M, et al.Regul.Toxicol.Pharm. such as spread effect, sensitization, mutagenesis; 2005,43:232-248).
For reducing formaldehyde to environment and healthy harm; The researcher has proposed many improvement methods, as: the concentration, the inhibition formaldehyde that reduce formaldehyde volatilize, eliminate (list of references 3:Coleman R, et al.J.Anat. such as formaldehyde odor, use formaldehyde substitute; 1998,192:443-446; List of references 4: Liu Yangbin, etc. Chinese Tissue Engineering Study and clinical rehabilitation, 2007,11:4625-4626; List of references 5: Ding Guofang, etc. environment and occupational medicine, 2003,20:451-453).
Application number is that 200810125376.X, 200510119111.5 Chinese invention patent disclose respectively with formaldehyde and glutaraldehyde is that liquid is preserved in master's embalming.Jiang Jiliang (200710034262.X) discloses a kind of preservative of being made up of main slaine, organic acid and crosslinking agent of anti-mildew effect by force; Cao Jianming (200710005074.4) discloses a kind of composition that is used for the preserving corpse samples after fixing the processing, is made up of heterocyclic compound, quaternary ammonium salt, Osmolyte regulator and NMF.
In field of funeral and interment, the Zhu Jinlong of Shanghai Inst. of Embalming (Fangfu Science and Technology Co Ltd, Shanhai), Ren Zuhan etc. preserve at remains and done many good tries aspect anticorrosion: it is the novel anticorrosion liquid that adopts materials preparations such as inorganic peroxy thing, glycerine, alcohol, plant (Chinese herbal medicine) extract (Chinese patent number 01132050.8) that its invention " Shen dragon board " remains are preserved impregnating fluid; One Chinese patent application CN200410066612.7 discloses the composition that is used for long-term Gentle Repose; CN200410053606.8 relates to a kind of composition that is used for the short-term Gentle Repose, oozes dehydrating agent etc. by hydrogen peroxide solution, glycerine, n-hexyl alcohol, Peracetic acid, organic acid, height and forms.Spain Complucad preservative does not contain formaldehyde, phenol yet, is main component with organic peroxide and ethanol.Foregoing preservatives has all been used peroxide, is prone to produce gas, cause the remains dilatancy, and its antiseptic effect is abundant inadequately in the many wet environments of high temperature.
For overcoming with formaldehyde, peroxide is the shortcoming of the Gentle Repose agent of main formula; Further improve the performance of existing preservative, adapt to the anticorrosion preservation requirement of various environment and region, press for that development is a kind of efficiently, the Gentle Repose agent of low toxicity, environmental protection; Through methods such as remains arterial perfusion and body cavity suction injections; Prevent the propagation of growing of the interior pathogenic microorganism of remains, the development of the self-dissolving of control remains, corruption is handled remains and is reached innoxious.The inventor carries out research experiment repeatedly, and preparation obtains a kind of short-term Gentle Repose agent of novel low toxic and environment-friendly.
Summary of the invention
The objective of the invention is to overcome the deficiency of prior art, a kind of formaldehyde and organic oxygen compound of not containing is provided, is main efficient, low toxicity, the Gentle Repose agent of environment-friendly type short-term and method for using thereof with the alcohols.To be applied to funeral service and preservation of remains medically or fixation of tissue.
The objective of the invention is to realize like this:
The agent of short-term Gentle Repose and the method for using thereof of a kind of low toxic and environment-friendly provided by the invention is characterized in that:
Gentle Repose agent of the present invention; Mainly by volume ratio be 60～75% ethanol, 5～20% 1,2-propane diols, 3～15% organic acid, 0～0.3% essence, 10～40% distilled water and weight ratio are that 0.2～10% nipagin esters, 0.3～3% thymol, 0.5～3% mannitol (or D-sorbierite), 0.05～0.5% low toxicity slaine constitute.
Nipagin esters in the preservative of the present invention comprises methyl hydroxybenzoate, ethylparaben, propylben and composition thereof; Organic acid is selected from acetate and propionic acid; Flavoring essences such as the preferred pineapple of essence, orange, the low toxicity slaine comprises calcium acetate, sodium hydrogen phosphate, magnesium chloride, sodium chloride, zinc sulphate, aluminum sulfate etc.
The preparation method of Gentle Repose agent of the present invention comprises the steps: by the prescription ratio nipagin esters to be dissolved in the ethanol, adds thymol, mannitol (or D-sorbierite), 1 successively, and the 2-propane diols stirs, fully dissolving; Add entry, low toxicity slaine, organic acid and essence then while stirring, mixing promptly gets.
Utilize Gentle Repose agent of the present invention; Can adopt infusion method or perfusion anticorrosion fixing to remains, tissue and dissection; Through sustainer, arch of aorta perfusion or chest, lumbar injection preservative, play, preserve, sterilize, dispel smelly effect effectively anticorrosion rapidly to remains.
Short-term Gentle Repose provided by the invention agent and method for using have following advantage:
This preservative is main with alcohols, does not contain formaldehyde, inorganic oxide and organic oxygen compound, and formaldehydeless penetrating odor is little to environment and human health risk, low toxicity, environmental protection.Its preparation method is easy, and raw material is easy to get, and is workable; Practical application is convenient, is beneficial to and promotes the use of.
In the Gentle Repose agent component of the present invention, contain preservative, bactericide, bleeding agent, NMF, solvent etc., prescription is reasonable, and ratio is appropriate.Wherein, nipagin esters (p-hydroxybenzoate) bactericide toxicity is extremely low, and is non-stimulated to human body, is usually used in being used for cosmetics, weaving and food additives; Nipagin esters can make the activity of microbial cell respiratory system enzyme and electron transport system enzyme suppressed, and the structure of ability destroy microorganisms cell membrane, thereby the antisepsis of playing all has antibacterial action widely to mould, yeast and bacterium.
When utilizing preservative of the present invention to handle remains, can discharge the remains inner blood, or not bloodletting; Remains after the processing only need under room temperature state, to preserve, and do not need refrigerator cold-storage.The anti-corrosion method of standard is otch, bloodletting in the world; If do not discharge the remains inner blood, do not have corpse liquid during operation and ooze out, free from environmental pollution, funeral belongs to easier acceptance.
Gentle Repose agent of the present invention can be used as the histocyte fixative, is applicable to the short-term Gentle Repose or the sample preservation of the domestic and international funeral parlor and medical colleges and schools.
Further describe the present invention below in conjunction with accompanying drawing and embodiment:
Description of drawings
Accompanying drawing 1 is: the mouse tissue form pathological change microscope figure of injection preservative of the present invention.
(A) brain, (B) heart, (C) liver, (D) lung, (E) kidney, (F) intestines, (G) skeletal muscle.
Embodiment
Embodiment 1: bacteria quantified is killed test and is killed test with fungi
For estimating the killing effect of preservative of the present invention,, carried out bacteria quantified respectively and killed test and kill test with fungi according to disinfection technology standard (version in 2002) to microorganism.Bacterial classification is selected: staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922, Candida albicans ATCC 10231.Are set at working concentration and action time dilution 20 times and 6h respectively.
By the working concentration and action time of appointment, repeated test 3 times, suspension is quantitatively killed test (referring to table 1), to staphylococcus aureus and each time of Escherichia coli kill logarithm value equal >=5.00; To each time of Candida albicans test kill logarithm value all >=4.00, it is qualified to be judged to be sterilization.
Each bacterium survival concentration of table 1 inoculation different time sections
Annotate: "/" expression does not detect
Embodiment 2: laboratory injected in mice antiseptic experiment
Use the speed of 0.1mL/1Kg to sleep after new II (for animals) puts to death one group of experiment mice; Press the dosage injection of heart preservative of 25mL/100g; Place the dry place of shady and cool ventilation, deposit 30 days (on June 4,2010 5 days～2010 May in) under the room temperature state, observe antiseptic effect.In experiment the 3rd day, 7 days, 14 days, 30 days the time, respectively get a mouse and dissect and draw materials, cut tissues such as skeletal muscle, intestines, the heart, lung, liver, kidney, brain respectively, fixing, paraffin section, hematoxylin eosin staining, microscopic examination is also taken a picture.This experiment is contrast (FineFIX is the novel no formalin fixed agent of carrying out morphology research of Italian Milestone patent) with the FineFIX preservative, with sterile saline as blank.Experiment mice is available from Military Medical Science Institute's Experimental Animal Center, and is male, body weight 150～200g.
Experimental result is following:
The overall appearance aspect, the 3rd day when dissected of physiological saline control group seen stink, and to the 7th day skin blackout, suppurating was prone to break, and it is serious to rot, and has foul odour; And stink does not all appear in the mouse of using preservative of the present invention and FineFIX preservative during to 30 days, and the phenomenon of leakage partly appears in the FineFIX mouse, and through outward appearance and anatomic observations such as mouse hardness, colors, preservative of the present invention significantly is superior to the FineFIX preservative.
Mouse tissue form pathological section microscope is the aspect as a result, and the mouse lung liver cell nuclear quantity of using preservative of the present invention explains that significantly more than the FineFix group the present invention significantly is superior to FineFIX.Visible from the mouse tissue form pathological change microscope figure (Fig. 1) of injection preservative of the present invention, the mouse brain institutional framework is roughly normal, and the clear or few part nuclear of the nuclear of nerve cell disappears, and a matter structure is clear; Glomerulus inner cell nuclear is clear, minority disappears, and renal cells nuclear is clear, minority disappears, and a matter structure is clear; Each layer of intestinal tissue is most of normal, and cell nucleus is most of clear; The most of normal or all disappearances of Skeletal Muscle Cell nuclear, myocyte's band is regional to disappear, and a matter is most of normal.
Embodiment 3: medical college's Gentle Repose experiment
Choose tool adult male remains (the about 60Kg of body weight) in certain medical university, arterial perfusion and the about 25000mL of splanchnocoel injection preservative of the present invention, close observation antiseptic experiment result.The result: room temperature was placed after 5 months, observed remains from outward appearance and did not have obvious corruption, no stink, and tissue morphology pathological section microscopic examination preservative of the present invention can keep the intrinsic form and the structure of cell, tissue basically.
Embodiment 4: funeral parlor Gentle Repose field experiment
Gentle Repose agent of the present invention is application 10 many cases (lift two examples at present and list in table 2) in the funeral parlor, can pass through the perfusion of sustainer, the arch of aorta or abdomen/chest/pharynx/cranial cavity injection, to remains carry out disinfection, anticorrosion and preservation.Efficient reaching more than 95% at room temperature 5 days can make the remains face keep the sleep shape, do not have decortication, and the chest belly does not have green dying and swelling, no stink.
Table 2 preservative of the present invention funeral parlor field experiment result
Claims (7)
1. the short-term Gentle Repose agent of a low toxic and environment-friendly; It is characterized in that preservative mainly by volume ratio be 60～75% ethanol, 5～20% 1,2-propane diols, 3～15% organic acid, 0～0.3% essence, 10～40% distilled water and weight ratio are that 0.2～10% nipagin esters, 0.3～3% thymol, 0.5～3% mannitol (or D-sorbierite), 0.05～0.5% low toxicity slaine constitute.
2. preservative according to claim 1 is characterized in that with alcohols being main, does not contain formaldehyde, inorganic oxide and organic oxygen compound.
3. preservative according to claim 1; It is characterized in that described nipagin esters comprises methyl hydroxybenzoate, ethylparaben, propylben and composition thereof; Organic acid is selected from acetate and propionic acid; Flavoring essences such as the preferred pineapple of essence, orange, the low toxicity slaine comprises calcium acetate, sodium hydrogen phosphate, magnesium chloride, sodium chloride, zinc sulphate, aluminum sulfate.
4. the preparation method of preservative according to claim 1 is characterized in that the preparation method of this preservative comprises the steps:
By the prescription ratio, nipagin esters is dissolved in the ethanol, add thymol, mannitol (or D-sorbierite), 1 successively, the 2-propane diols stirs, fully dissolving; Add entry, low toxicity slaine, organic acid and essence then while stirring, mixing promptly gets.
5. preservative according to claim 1; It is characterized in that adopting infusion method or perfusion anticorrosion fixing to remains, tissue and dissection; Through sustainer, arch of aorta perfusion or chest, lumbar injection preservative, play, preserve, sterilize, dispel smelly effect effectively anticorrosion rapidly to remains.
6. preservative according to claim 1 when it is characterized in that using this preservative to carry out the remains processing, can discharge the remains inner blood, or not bloodletting, should be decided according to the actual requirements.
7. preservative according to claim 1 can be used as the histocyte fixative, is applicable to the short-term Gentle Repose or the sample preservation of the domestic and international funeral parlor and medical colleges and schools.
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Cited By (11)
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| CN103988834A (en) * | 2014-05-18 | 2014-08-20 | 南阳医学高等专科学校 | Animal specimen preserving fluid |
| CN104094923A (en) * | 2013-04-09 | 2014-10-15 | 民政部一零一研究所 | Preservative liquid for corpses, tissue and dissections, and application method thereof |
| CN105010303A (en) * | 2015-07-10 | 2015-11-04 | 毕爱胜 | Corruption remains restoring method |
| CN105842033A (en) * | 2016-05-13 | 2016-08-10 | 北京九州柏林生物科技有限公司 | Formaldehyde-free tissue specimen stationary liquid |
| CN107560904A (en) * | 2016-07-01 | 2018-01-09 | 山东大学齐鲁医院 | A kind of urine anti-corrosion method for urine chemistries analysis |
| CN109042621A (en) * | 2018-08-01 | 2018-12-21 | 张炳强 | A kind of dedicated short-term corrosion prevention liquid of gross specimen and preparation method thereof |
| CN110169417A (en) * | 2019-04-30 | 2019-08-27 | 黄恩南 | Deoderizing Gentle Repose liquid |
| CN110771596A (en) * | 2019-11-21 | 2020-02-11 | 南通大学 | Preservation method for fresh corpses |
| CN111296408A (en) * | 2020-02-17 | 2020-06-19 | 广东海洋大学深圳研究院 | Specimen environment-friendly composite mildew-proof preservative of natural plant extract and application thereof |
| CN111387199A (en) * | 2020-02-17 | 2020-07-10 | 广东海洋大学深圳研究院 | Efficient composite mildew-proof preservative and application thereof |
| CN114503977A (en) * | 2022-01-26 | 2022-05-17 | 民政部一零一研究所 | Remains preservative with detumescence, fading and odor removing functions and preparation method thereof |
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Cited By (13)
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| CN104094923A (en) * | 2013-04-09 | 2014-10-15 | 民政部一零一研究所 | Preservative liquid for corpses, tissue and dissections, and application method thereof |
| CN104094923B (en) * | 2013-04-09 | 2016-06-15 | 民政部一零一研究所 | A kind of antiseptic preserving fluid for corpse, tissue and dissection and using method thereof |
| CN103988834A (en) * | 2014-05-18 | 2014-08-20 | 南阳医学高等专科学校 | Animal specimen preserving fluid |
| CN105010303A (en) * | 2015-07-10 | 2015-11-04 | 毕爱胜 | Corruption remains restoring method |
| CN105842033A (en) * | 2016-05-13 | 2016-08-10 | 北京九州柏林生物科技有限公司 | Formaldehyde-free tissue specimen stationary liquid |
| CN107560904A (en) * | 2016-07-01 | 2018-01-09 | 山东大学齐鲁医院 | A kind of urine anti-corrosion method for urine chemistries analysis |
| CN109042621A (en) * | 2018-08-01 | 2018-12-21 | 张炳强 | A kind of dedicated short-term corrosion prevention liquid of gross specimen and preparation method thereof |
| CN110169417A (en) * | 2019-04-30 | 2019-08-27 | 黄恩南 | Deoderizing Gentle Repose liquid |
| CN110771596A (en) * | 2019-11-21 | 2020-02-11 | 南通大学 | Preservation method for fresh corpses |
| CN111296408A (en) * | 2020-02-17 | 2020-06-19 | 广东海洋大学深圳研究院 | Specimen environment-friendly composite mildew-proof preservative of natural plant extract and application thereof |
| CN111387199A (en) * | 2020-02-17 | 2020-07-10 | 广东海洋大学深圳研究院 | Efficient composite mildew-proof preservative and application thereof |
| CN114503977A (en) * | 2022-01-26 | 2022-05-17 | 民政部一零一研究所 | Remains preservative with detumescence, fading and odor removing functions and preparation method thereof |
| CN114503977B (en) * | 2022-01-26 | 2023-03-03 | 民政部一零一研究所 | Remains preservative with detumescence, fading and odor removing functions and preparation method thereof |
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