CN102461496B - Low-toxicity, environment-friendly short-term remains preservative and usage thereof - Google Patents
Low-toxicity, environment-friendly short-term remains preservative and usage thereof Download PDFInfo
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Abstract
The invention relates to a low-toxicity, environment-friendly short-term remains preservative based on alcohols and usage thereof. The remains preservative mainly comprises 60 to 75 percent of ethanol, 5 to 20 percent of propylene glycol, 3 to 15 percent of organic acid, 0 to 0.3 percent of essence and 10 to 40 percent of distilled water according to volumetric proportion as well as 0.2 to 10 percent of nipagin ester, 0.3 to 3 percent of thymol, 0.5 to 3 percent of mannitol (or sorbierite) and 0.05 to 0.5 percent of low-toxicity metal salt according to weight ratio. The invention can adopt the immersion method or the infusion method to preserve and fix remains (tissues), and the preservative can be infused via the aorta (arch) and injected via the thoracic cavity and the abdominal cavity, and has the fast and effective effects of disinfection, embalmment and preservation. The preservative is highly antibacterial, can effectively prevent bacteria from corroding the remains and the autolysis of the tissues, can be used as histocyte fixative, and is applicable to short-term remains embalmment or specimen preservation in domestic and foreign funeral homes and medical academies.
Description
Technical field
The present invention relates to the Gentle Repose Techniques of preserving in field of funeral and interment and human dissection teaching and scientific research field, be specifically related to a kind of agent of short-term Gentle Repose and using method thereof of low toxic and environment-friendly.
Background technology
After death, if corpse is without anticorrosion fixing processing, will soon there is tissue automatic soup-dissolving in human or animal, corruption is disintegrated gradually.For preventing the corrosion of bacterium and the self-dissolving of tissue, must carry out anticorrosion fixing processing to remains (corpse), tissue and dissection.Gentle Repose refers to and within a period of time, adopts the method such as physics, chemistry to prevent or slow down further developing of the corrupt process of rotting of remains, make remains be kept at relatively good state and can prevent contingent disease propagation, to carry out funeral activities or anatomic teaching scientific research.
At present, conventional anticorrosion fixed drug mainly contains: formaldehyde, ethanol, glycerine, phenol etc., and its anticorrosion fixing general principle comprises: protein denaturation is solidified; Disturb the important enzyme system of microorganism; Destroy its permeability of bacterial cell membrane change etc.Because antiseptic property and the method for every kind of medicament are different, respectively there are its pluses and minuses, in order to strengthen its effect, make up its defect, often by multiple medications mixed preparing, to obtain desirable effect.The existing summary of remains (corpse) preservative present Research deliver (bibliography 1: Zhou Xuemei, etc. dissect scientific advance, 2008,14:457-460).Generally adopt the main component of formalin (being formalin) as Gentle Repose agent prescription both at home and abroad, as Chinese patent CN200810125376.X and U.S. Dodge series preservative etc.But formaldehyde and antiseptic fixation exist organize hard and crisp, elasticity is little, anti-mold effect is poor, and there is strong impulse smell, human body is had to serious harm (the bibliography 2:Naya M such as spread effect, sensitization, mutagenesis, et al.Regul.Toxicol.Pharm., 2005,43:232-248).
For reducing formaldehyde to environment and healthy harm, researcher has proposed many improvement methods, as: the concentration, the inhibition formaldehyde that reduce formaldehyde volatilize, eliminate formaldehyde odor, use (the bibliography 3:Coleman R such as formaldehyde substitute, et al.J.Anat., 1998,192:443-446; Bibliography 4: Liu Yangbin, etc. Chinese Tissue Engineering Study and clinical rehabilitation, 2007,11:4625-4626; Bibliography 5: Ding Guofang, etc. environment and occupational medicine, 2003,20:451-453).
Application number is that 200810125376.X, 200510119111.5 Chinese invention patent disclose respectively taking formaldehyde and glutaraldehyde as main embalming and preserve liquid.Jiang Jiliang (200710034262.X) discloses a kind of preservative of the stronger anti-mildew effect being made up of main slaine, organic acid and crosslinking agent; Cao Jianming (200710005074.4) discloses a kind of for the composition through fixing preserving corpse samples after treatment, is made up of heterocyclic compound, quaternary ammonium salt, Osmolyte regulator and humectant.
In field of funeral and interment, Zhu Jinlong, the Ren Zuhan etc. of Shanghai Inst. of Embalming (Fangfu Science and Technology Co Ltd, Shanhai) preserves and done many good tries aspect anticorrosion at remains: it is the novel anticorrosion liquid (China Patent No. 01132050.8) that adopts the material formulations such as inorganic peroxy thing, glycerine, alcohol, plant (Chinese herbal medicine) extract that its invention " Shen dragon board " remains are preserved impregnating fluid; Chinese patent application CN200410066612.7 discloses the composition for long-term Gentle Repose, CN200410053606.8 relates to a kind of composition for short-term Gentle Repose, is made up of hydrogen peroxide, glycerine, n-hexyl alcohol, Peracetic acid, organic acid, hyperosmotic dehydrant etc.Spain Complucad preservative is also containing formaldehyde, phenol, taking organic peroxide and ethanol as main component.Foregoing preservatives has all been used peroxide, easily produces gas, causes remains dilatancy, and its antiseptic effect is abundant not in high temperature and humidity environment.
For overcoming the shortcoming taking formaldehyde, peroxide as the Gentle Repose agent of main formula, further improve the performance of existing preservative, adapt to the anticorrosion preservation requirement of various environment and region, in the urgent need to developing the Gentle Repose agent of a kind of efficient, low toxicity, environmental protection, by methods such as remains arterial perfusion and body cavity suction injections, prevent the propagation of growing of the interior pathogenic microorganism of remains, control the development of remains self-dissolving, corruption, make remains processing reach innoxious.The inventor carries out research experiment repeatedly, and preparation obtains a kind of short-term Gentle Repose agent of novel low toxic and environment-friendly.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, provide one containing formaldehyde and organic oxygen compound, taking alcohols as main efficient, low toxicity, the Gentle Repose agent of environment-friendly type short-term and using method thereof.To be applied to, funeral service and remains are medically preserved or tissue is fixing.
The object of the present invention is achieved like this:
The agent of short-term Gentle Repose and the using method thereof of a kind of low toxic and environment-friendly provided by the invention, is characterized in that:
Gentle Repose agent of the present invention, the nipagin esters that the main ethanol that is 60～75% by volume ratio, 5～20% 1,2-PD, 3～15% organic acid, 0～0.3% essence, 10～40% distilled water and weight ratio are 0.2～10%, 0.3～3% thymol, 0.5～3% mannitol (or D-glucitol), 0.05～0.5% low toxicity slaine form.
Nipagin esters in preservative of the present invention comprises methyl hydroxybenzoate, ethylparaben, propylben and composition thereof, organic acid is selected from acetic acid and propionic acid, the flavoring essences such as the preferred pineapple of essence, orange, low toxicity slaine comprises calcium acetate, sodium hydrogen phosphate, magnesium chloride, sodium chloride, zinc sulphate, aluminum sulfate etc.
The preparation method of Gentle Repose agent of the present invention comprises the steps: by recipe ratio, and nipagin esters is dissolved in ethanol, adds successively thymol, mannitol (or D-glucitol), 1,2-PD, stirs, and fully dissolves; Then add while stirring water, low toxicity slaine, organic acid and essence, mix and get final product.
Utilize Gentle Repose agent of the present invention, can adopt infusion method or perfusion anticorrosion fixing to remains, tissue and dissection, by sustainer, arch of aorta perfusion or chest, lumbar injection preservative, play remains effectively anticorrosion, the smelly effect of preserving, sterilize, dispel rapidly.
Short-term Gentle Repose provided by the invention agent and using method, have following advantage:
This preservative, taking alcohols as main, does not contain formaldehyde, inorganic oxide and organic oxygen compound, and formaldehydeless penetrating odor is little to environment and human health risk, low toxicity, environmental protection.Its preparation method is easy, and raw material is easy to get, workable; Practical application is convenient, is beneficial to and promotes the use of.
In Gentle Repose agent component of the present invention, contain preservative, bactericide, bleeding agent, humectant, solvent etc., rationally, ratio is appropriate for formula.Wherein, nipagin esters (p-hydroxybenzoate) bactericide toxicity is extremely low, non-stimulated to human body, is usually used in for cosmetics, weaving and food additives; Nipagin esters can make the activity of microbial cell respiratory system enzyme and electron transport system enzyme suppressed, and the structure of energy destroy microorganisms cell membrane, thereby the antisepsis of playing all has antibacterial action widely to mould, yeast and bacterium.
While utilizing preservative of the present invention to process remains, can discharge remains inner blood, or not bloodletting; Remains after treatment only need be preserved under room temperature state, do not need refrigerator cold-storage.The anti-corrosion method of standard is otch, bloodletting in the world; If do not discharge remains inner blood, when operation, ooze out without corpse liquid, free from environmental pollution, funeral belongs to more easily to be accepted.
Gentle Repose agent of the present invention, can be used as histocyte fixative, and the short-term Gentle Repose or the sample that are applicable to the domestic and international funeral parlor and medical colleges and schools are preserved.
Further describe the present invention below in conjunction with drawings and Examples:
Brief description of the drawings
Accompanying drawing 1 is: the mouse tissue form pathological change microscope figure of injection preservative of the present invention.
(A) brain, (B) heart, (C) liver, (D) lung, (E) kidney, (F) intestines, (G) skeletal muscle.
Embodiment
Embodiment 1: Guantitative bactericidal test and fungi are killed test
For evaluating the killing effect of preservative of the present invention to microorganism, according to disinfection technology standard (version in 2002), carry out respectively Guantitative bactericidal test and fungi and killed test.Bacterial classification is selected: staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922, Candida albicans ATCC 10231.Are set as respectively working concentration and action time diluting 20 times and 6h.
By working concentration and the action time of specifying, repeated test 3 times, suspension is quantitatively killed test (referring to table 1), to each time of staphylococcus aureus and Escherichia coli kill logarithm value equal >=5.00; What Candida albicans was tested for each time kills logarithm value all >=4.00, is judged to be to sterilize qualified.
Table 1 is inoculated the each bacterium survival of different time sections concentration
Note: "/" represents not detect
Embodiment 2: the anticorrosion experiment of laboratory injected in mice
Use the speed of 0.1mL/1Kg to sleep after new II (for animals) execution one group of experiment mice, press the dosage injection of heart preservative of 25mL/100g, be placed in the dry place of shady and cool ventilation, under room temperature state, deposit 30 days (on June 4,5 days～2010 May in 2010), observe antiseptic effect.In experiment the 3rd day, 7 days, 14 days, 30 days time, respectively get a mouse and dissects and draw materials, cut respectively the tissues such as skeletal muscle, intestines, the heart, lung, liver, kidney, brain, fixing, paraffin section, hematoxylin eosin staining, microscopic examination photograph.This experiment is taking FineFIX preservative as contrast (FineFIX be Italian Milestone patent carry out the novel without formalin fixative of morphology research), using sterile saline as blank.Experiment mice is purchased from Military Medical Science Institute's Experimental Animal Center, male, body weight 150～200g.
Experimental result is as follows:
Overall appearance aspect, the 3rd day when dissected of physiological saline control group has been shown in stink, to the 7th day skin blackout, suppurates easily broken, rots serious, has foul odour; And stink does not all appear in the mouse of use preservative of the present invention and FineFIX preservative during to 30 days, there is the phenomenon of leakage in FineFIX mouse part, and by outward appearance and anatomic observations such as mouse hardness, colors, preservative of the present invention is significantly better than FineFIX preservative.
Mouse tissue form pathological section microscope result aspect, is used the mouse lung liver cell nuclear quantity of preservative of the present invention significantly more than FineFix group, illustrates that the present invention is significantly better than FineFIX.From the mouse tissue form pathological change microscope figure (Fig. 1) of injection preservative of the present invention, mouse brain institutional framework is roughly normal, the clear or small part core disappearance of the core of nerve cell, and interstitial structure is clear; Glomerulus inner cell core is clear, minority disappears, and renal cells core is clear, minority disappears, and interstitial structure is clear; Each layer of great majority of intestinal tissue are normal, and cell nucleus is most of clear; Skeletal Muscle Cell core major part normally or all disappears, and myocyte's band is regional to disappear, and interstitial is most of normal.
Embodiment 3: medical college's Gentle Repose experiment
Choose tool adult male remains (the about 60Kg of body weight) in certain medical university, arterial perfusion and the about 25000mL of splanchnocoel injection preservative of the present invention, the anticorrosion experimental result of close observation.Result: room temperature was placed after 5 months, observes remains without obviously corruption, odorless from outward appearance, and tissue morphology pathological section microscopic examination preservative of the present invention can keep intrinsic form and the structure of cell, tissue substantially.
Embodiment 4: funeral parlor Gentle Repose field experiment
10 many cases (now lift two examples and list in table 2) are applied in Gentle Repose agent of the present invention in the funeral parlor, can pass through the perfusion of sustainer, the arch of aorta or abdomen/chest/pharynx/cranial cavity injection, to remains carry out disinfection, anticorrosion and preservation.Efficient reaching more than 95% at room temperature 5 days, can make remains face keep sleep shape, and without decortication, chest belly dyes and swelling without green, odorless.
Table 2 preservative of the present invention funeral parlor field experiment result
Claims (3)
1. the short-term Gentle Repose agent of a low toxic and environment-friendly, it is characterized in that the nipagin esters that ethanol that preservative is mainly 60～75% by volume ratio, 5～20% 1,2-PD, 3～15% organic acid, 0～0.3% essence, 10～40% distilled water and weight ratio are 0.2～10%, 0.3～3% thymol, 0.5～3% mannitol or D-glucitol, 0.05～0.5% low toxicity slaine form; Described preservative, is set as respectively working concentration and action time diluting 20 times and 6h;
Described nipagin esters is methyl hydroxybenzoate, ethylparaben, propylben and composition thereof, organic acid is selected from acetic acid and propionic acid, essence is pineapple, orange flavoring essence, and low toxicity slaine is calcium acetate, sodium hydrogen phosphate, magnesium chloride, sodium chloride, zinc sulphate, aluminum sulfate.
2. according to the preparation method of the preservative described in claim l, it is characterized in that the preparation method of this preservative comprises the steps:
By recipe ratio, nipagin esters is dissolved in ethanol, add successively thymol, mannitol or D-glucitol, 1,2-PD, stir, fully dissolve; Then add while stirring water, low toxicity slaine, organic acid and essence, mix and get final product.
3. preservative according to claim 1, it is characterized in that adopting infusion method or perfusion anticorrosion fixing to remains, tissue and dissection, by sustainer, arch of aorta perfusion or chest, lumbar injection preservative, play remains effectively anticorrosion, the smelly effect of preserving, sterilize, dispel rapidly.
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| CN104094923B (en) * | 2013-04-09 | 2016-06-15 | 民政部一零一研究所 | A kind of antiseptic preserving fluid for corpse, tissue and dissection and using method thereof |
| CN103988834A (en) * | 2014-05-18 | 2014-08-20 | 南阳医学高等专科学校 | Animal specimen preserving fluid |
| CN105010303A (en) * | 2015-07-10 | 2015-11-04 | 毕爱胜 | Corruption remains restoring method |
| CN105842033A (en) * | 2016-05-13 | 2016-08-10 | 北京九州柏林生物科技有限公司 | Formaldehyde-free tissue specimen stationary liquid |
| CN107560904A (en) * | 2016-07-01 | 2018-01-09 | 山东大学齐鲁医院 | A kind of urine anti-corrosion method for urine chemistries analysis |
| CN109042621A (en) * | 2018-08-01 | 2018-12-21 | 张炳强 | A kind of dedicated short-term corrosion prevention liquid of gross specimen and preparation method thereof |
| CN110169417A (en) * | 2019-04-30 | 2019-08-27 | 黄恩南 | Deoderizing Gentle Repose liquid |
| CN110771596A (en) * | 2019-11-21 | 2020-02-11 | 南通大学 | Preservation method for fresh corpses |
| CN111387199A (en) * | 2020-02-17 | 2020-07-10 | 广东海洋大学深圳研究院 | Efficient composite mildew-proof preservative and application thereof |
| CN111296408A (en) * | 2020-02-17 | 2020-06-19 | 广东海洋大学深圳研究院 | Specimen environment-friendly composite mildew-proof preservative of natural plant extract and application thereof |
| CN114503977B (en) * | 2022-01-26 | 2023-03-03 | 民政部一零一研究所 | Remains preservative with detumescence, fading and odor removing functions and preparation method thereof |
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