CN105842033A - Formaldehyde-free tissue specimen stationary liquid - Google Patents
Formaldehyde-free tissue specimen stationary liquid Download PDFInfo
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- CN105842033A CN105842033A CN201610319527.XA CN201610319527A CN105842033A CN 105842033 A CN105842033 A CN 105842033A CN 201610319527 A CN201610319527 A CN 201610319527A CN 105842033 A CN105842033 A CN 105842033A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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Abstract
The invention discloses formaldehyde-free tissue specimen stationary liquid. The stationary liquid is composed of a mixed solution and additive components. The mixed solution is prepared from, by volume, 45-50 parts of anhydrous ethanol, 20-25 parts of absolute methanol, 15 parts of polyethylene glycol-400, 10-20 parts of purified water and 0.03-0.05 part of surfactant. The additive components include anhydrous sodium carbonate with the final concentration in the stationary liquid being 3-5 g/L and trichloroacetic acid with the final concentration in the stationary liquid being 6-8 g/L. The formaldehyde-free tissue specimen stationary liquid is prepared from non-toxic and harmless chemical reagents and is good in fixing effect, and shape of tissue cells is preserved completely.
Description
Technical field
The invention belongs to pathological experiment technical field, relate to external diagnosis reagent, particularly relate to a kind of nothing
Formaldehyde fixative for tissue sample.
Background technology
The histopathologic birth history of existing more than 150 year, in all of hospital, the disease of surgery excision
Reason sample and various biopsy, made histopathology by hospital pathology department to operation tissue samples
Learn diagnosis.
Routine paraffin wax embedding HE stained preparation is the current most basic technology in order to handling tissue samples, it
It is the basis of pathological diagnosis method, is also many new methods, the evaluation reference standard of new technology quality.
Traditional method is in the cylinder that tissue samples is placed treatment fluid, pours into successively or calls in different disposal liquid,
Sample soaks different time by regulation successively in each reagent to be completed to process section, dyeing, mounting etc.,
More than 30 step of omnidistance needs.Whole process uses formaldehyde, dimethylbenzene, mercury oxide, ammoniacal liquor poisonous in a large number
Evil chemical reagent, pathology technicians places oneself in the midst of toxic environment, and reagent emission of serious pollution of environment for a long time.
And reagent tolerance used is poor, operation complexity, reagent consumption is big, and pathology film-making high-quality sheet is low (complete
State adds up 40～70%), have a strong impact on pathological diagnosis.Jue great Shuo hospital joins reagent voluntarily simultaneously, lacks
Unified standard.Therefore the standardized production of pathology industry organization sample disposal liquid articles for use, Non-toxic, society
Change supply and demand, be the inexorable trend of industry development.
It is normal that the patent application of such as Application No. 200810136961.X discloses a kind of biological organization sample
Rule paraffin wax flaking HE dyeing non-xylol whole-course treatment liquid.But this place fixing based on tradition tissue
Reason liquid still have employed the murder by poisoning reagent formaldehyde of serious environment pollution.Use the formaldehyde-formal of variable concentrations
Woods, fixing human and animal vegetable tissue sample, carried out Human pathology and the pathological research of animals and plants
There is the history of more than 160 years.Formaldehyde is that the poisonous and harmful chemicals that the world today generally acknowledges has potential cause
Carcinous, it is a kind of simple fixative, and its fixed mechanism is by protein subunit amino acid molecular
Between hinge reaction, formed methylene bridge complete fixation.But cross-linking reaction there will be contraction hardening
Etc. phenomenon, histiocytic antigen molecule structure, enzyme, nucleic acid molecules are preserved the best.Therefore, grind
Send out the substitute of formaldehyde, substitute formaldehyde and have great importance for pathological experiment.
Summary of the invention
The present invention has designed and developed the formaldehyde-free fixative for tissue sample of a kind of nonhazardous, good fixing effect.
The technical scheme that the present invention provides is:
A kind of formaldehyde-free fixative for tissue sample, it is made up of mixed solution and addO-on therapy, wherein, mixed
Close solution and comprise the component of volumes below number: absolute ethyl alcohol 45～50 parts, absolute methanol 20～25 parts,
PEG-4000 15 parts, purified water 10～20 parts and surfactant 0.03～0.05 part, described
AddO-on therapy comprises following components, final concentration of in described fixer of each component: natrium carbonicum calcinatum 3～
5g/L and trichloroacetic acid 6～8g/L.
Preferably, in described formaldehyde-free fixative for tissue sample, it is by mixed solution and addO-on therapy
Composition, wherein, mixed solution comprises the component of volumes below number: absolute ethyl alcohol 45 parts, absolute methanol
25 parts, PEG-4000 15 parts, purified water 15 parts and surfactant 0.03～0.05 part, institute
State addO-on therapy and comprise following components, final concentration of in described fixer of each component: natrium carbonicum calcinatum
3g/L and trichloroacetic acid 7.5g/L.
Preferably, in described formaldehyde-free fixative for tissue sample, described surfactant is fluorine class table
Face activating agent.
Preferably, in described formaldehyde-free fixative for tissue sample, described mixed solution also comprises poly-second
Glycol-200 5 parts, Macrogol 600 5 parts and polypropylene glycol-425 3 parts.
Formaldehyde-free fixative for tissue sample of the present invention uses nontoxic chemical reagent to make, Gu
Fixed effective, histiocytic form preserves complete.The formaldehyde-free fixative for tissue sample of the present invention can be expired
Foot routine pathology diagnosis, immunohistochemical diagnosis technology, diagnostic technique in molecular biology requirement, process
Tissue effect be better than conventional art, to substitute the pathological experiment that formaldehyde is applied to be correlated with completely.
Detailed description of the invention
The present invention is described in further detail below, to make those skilled in the art with reference to specification literary composition
Word can be implemented according to this.
The present invention provides a kind of formaldehyde-free fixative for tissue sample, and it is made up of mixed solution and addO-on therapy,
Wherein, mixed solution comprises the component of volumes below number: absolute ethyl alcohol 45～50 parts, absolute methanol
20～25 parts, PEG-4000 15 parts, purified water 10～20 parts and surfactant 0.03～0.05
Part, described addO-on therapy comprises following components, final concentration of in described fixer of each component: anhydrous
Sodium carbonate 3～5g/L and trichloroacetic acid 6～8g/L.
The present invention does not use formaldehyde, has both avoided the toxic action of formaldehyde, eliminates again the formaldehyde antagonism original
The covering of matter, it is possible to well preserve antigen.And the present invention uses PEG-4000 to promote molecule
Osmosis, to promote that cell protein precipitation completes fixation procedure, this process inhibits crosslinking
The phenomenons, the beneficially preservation of histocyte form such as the contraction hardening of reaction.
The formaldehyde-free fixative for tissue sample that the present invention provides uses composite parts formula, and its whole components are
Nontoxic chemical reagent；Through cytomorphology experiment, immunohistochemical assay, molecular pathology
Experiment and nucleic acid extraction are it is demonstrated experimentally that have more comprehensively fixed effect without aldehyde fixer, the most permissible
Substitute the pathological experiment that formaldehyde is applied to be correlated with, to complete the fixation to tissue specimen.
The preparation of the formaldehyde-free fixative for tissue sample that the present invention provides, is at normal temperatures by institute by each component
The ratio of stating mixes.
In a preferred embodiment, in described formaldehyde-free fixative for tissue sample, it is molten by mixing
Liquid and addO-on therapy composition, wherein, mixed solution comprises the component of volumes below number: absolute ethyl alcohol 45
Part, absolute methanol 25 parts, PEG-4000 15 parts, purified water 15 parts and surfactant 0.03～
0.05 part, described addO-on therapy comprises following components, final concentration of in described fixer of each component:
Natrium carbonicum calcinatum 3g/L and trichloroacetic acid 7.5g/L.
In a preferred embodiment, in described formaldehyde-free fixative for tissue sample, live in described surface
Property agent is fluorine class surfactant.
In a preferred embodiment, in described formaldehyde-free fixative for tissue sample, described mixing is molten
Liquid also comprises polyethylene glycol-200 5 parts, Macrogol 600 5 parts and polypropylene glycol-425 3 parts.
It has been investigated that, the interphase interaction of the polyethylene glycol of different molecular weight, it is more conducive to promote molecule
Osmosis, thus promote that cell protein precipitates.It addition, a small amount of polypropylene glycol-425 can enter one
The contraction hardening of step suppression cross-linking reaction, it is ensured that cell not contraction distortion, at utmost preserves histocyte
Form.By accurately regulating the consumption of polyethylene glycol-200, PEG-4000, polyethylene glycol-600,
The stability of tissue can also be strengthened, make to be organized in dehydration, embed, cut into slices and dyeing course is not damaged
Bad, and improve the structural constituent affinity to dyestuff, make Color good.
In order to further illustrate technical scheme, three embodiments presented below.
Embodiment one
In the present embodiment, formaldehyde-free fixative for tissue sample is made up of mixed solution and addO-on therapy, wherein,
Mixed solution comprises the component of volumes below number: absolute ethyl alcohol 45mL, absolute methanol 20mL, poly-second
Glycol-400 15mL, purified water 10mL and fluorine class surfactant 0.03mL, described addO-on therapy
Comprise following components, final concentration of in described fixer of each component: natrium carbonicum calcinatum 3g/L and trichlorine
Acetic acid 6g/L.
The fixer of application the present embodiment, histiocytic good fixing effect, cell is without deformation, cell shape
State preserves complete.
Embodiment two
In the present embodiment, formaldehyde-free fixative for tissue sample is made up of mixed solution and addO-on therapy, wherein,
Mixed solution comprises the component of volumes below number: absolute ethyl alcohol 50mL, absolute methanol 25mL, poly-second
Glycol-400 15mL, purified water 20mL and fluorine class surfactant 0.05mL, described addO-on therapy
Comprise following components, final concentration of in described fixer of each component: natrium carbonicum calcinatum 5g/L and trichlorine
Acetic acid 8g/L.
The fixer of application the present embodiment, histiocytic good fixing effect, cell is without deformation, cell shape
State preserves complete.
Embodiment three
In the present embodiment, formaldehyde-free fixative for tissue sample is made up of mixed solution and addO-on therapy, wherein,
Mixed solution comprises the component of volumes below number: absolute ethyl alcohol 45mL, absolute methanol 25mL, poly-second
Glycol-400 15mL, polyethylene glycol-200 5mL, Macrogol 600 5mL, polypropylene glycol-425 3mL,
Purified water 15mL and fluorine class surfactant 0.03mL, described addO-on therapy comprises following components, respectively
Final concentration of in described fixer of component: natrium carbonicum calcinatum 3g/L and trichloroacetic acid 7.5g/L.
The fixer of application the present embodiment, histiocytic good fixing effect, cell is without deformation, cell shape
State preserves complete.
Although embodiment of the present invention are disclosed as above, but it is not restricted to specification and embodiment party
Listed utilization in formula, it can be applied to various applicable the field of the invention completely, for being familiar with ability
For the personnel in territory, be easily achieved other amendment, therefore without departing substantially from claim and etc. homotype
Enclosing under limited universal, the present invention is not limited to specific details.
Claims (4)
1. a formaldehyde-free fixative for tissue sample, it is characterised in that it is by mixed solution and addO-on therapy
Composition, wherein, mixed solution comprises the component of volumes below number: absolute ethyl alcohol 45～50 parts, anhydrous
Methyl alcohol 20～25 parts, PEG-4000 15 parts, purified water 10～20 parts and surfactant 0.03～
0.05 part, described addO-on therapy comprises following components, final concentration of in described fixer of each component:
Natrium carbonicum calcinatum 3～5g/L and trichloroacetic acid 6～8g/L.
2. formaldehyde-free fixative for tissue sample as claimed in claim 1, it is characterised in that it is by mixing
Solution and addO-on therapy composition, wherein, mixed solution comprises the component of volumes below number: absolute ethyl alcohol
45 parts, absolute methanol 25 parts, PEG-4000 15 parts, purified water 15 parts and surfactant
0.03～0.05 part, described addO-on therapy comprises following components, and each component is dense for the end in described fixer
Degree is: natrium carbonicum calcinatum 3g/L and trichloroacetic acid 7.5g/L.
3. formaldehyde-free fixative for tissue sample as claimed in claim 1, it is characterised in that described surface
Activating agent is fluorine class surfactant.
4. formaldehyde-free fixative for tissue sample as claimed in claim 2 or claim 3, it is characterised in that described
Mixed solution also comprises polyethylene glycol-200 5 parts, Macrogol 600 5 parts and polypropylene glycol-425 3 parts.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610319527.XA CN105842033A (en) | 2016-05-13 | 2016-05-13 | Formaldehyde-free tissue specimen stationary liquid |
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| CN201610319527.XA CN105842033A (en) | 2016-05-13 | 2016-05-13 | Formaldehyde-free tissue specimen stationary liquid |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106706395A (en) * | 2016-12-08 | 2017-05-24 | 武汉宏兹生物技术有限公司 | Novel environment-friendly stationary liquid |
| CN108918213A (en) * | 2018-05-16 | 2018-11-30 | 浙江思格医疗科技有限公司 | A kind of palmitin fixer |
| CN109805001A (en) * | 2017-11-22 | 2019-05-28 | 哈尔滨格林标本技术开发有限公司 | Formaldehydeless tissue Fixed and Antiseptic liquid of one kind and preparation method thereof |
| CN110779785A (en) * | 2019-11-15 | 2020-02-11 | 北京雷根生物技术有限公司 | Novel formaldehyde-free tissue fixing solution |
| CN112067382A (en) * | 2020-08-07 | 2020-12-11 | 佛山科学技术学院 | Preparation method of small intestine slices |
| CN117419990A (en) * | 2023-10-27 | 2024-01-19 | 安徽合森医学检验有限公司 | Environment-friendly formaldehyde-free tissue fixing liquid |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112067382A (en) * | 2020-08-07 | 2020-12-11 | 佛山科学技术学院 | Preparation method of small intestine slices |
| CN117419990A (en) * | 2023-10-27 | 2024-01-19 | 安徽合森医学检验有限公司 | Environment-friendly formaldehyde-free tissue fixing liquid |
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Application publication date: 20160810 |