CN109452261A - A kind of specimen preserving liquid and preparation method thereof - Google Patents
A kind of specimen preserving liquid and preparation method thereof Download PDFInfo
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- CN109452261A CN109452261A CN201811612730.1A CN201811612730A CN109452261A CN 109452261 A CN109452261 A CN 109452261A CN 201811612730 A CN201811612730 A CN 201811612730A CN 109452261 A CN109452261 A CN 109452261A
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- specimen
- liquid
- preservation
- preserving liquid
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
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- General Health & Medical Sciences (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of specimen preserving liquid and preparation method thereof, the preservation liquid is mainly made of benzoic acid, ethyl alcohol, potassium nitrate, iron chloride, glycerol and water.Manufactured preservation liquid is free of formaldehyde, and volatilization is slow, has no irritating odor, toxicity is very low, highly-safe, and preservation effect is good, long period preservation will not go mouldy, and can accomplish the original fixation of sample, not injury tissue structure, and good color stabilizer effect can be played, Saving specimen is complete, skin histology not blackening, and it is soft, it has no irritating odor, sample sections observation, cell membrane saves completely, and institutional framework is clear, and muscle band is high-visible.
Description
Technical field
The invention belongs to field of medical biology, specifically, being related to a kind of specimen preserving liquid and preparation method thereof, more
Body is related to a kind of human body specimen and saves liquid and preparation method thereof.
Background technique
In medical anatomy, pathology, human body specimen plays an important role, therefore fully saves human body specimen
Importance it is self-evident.Currently, there are many kinds of the domestic and international methods for saving human body specimen, including cryo-conservation, liquid
Preservation, dehydrated preservation etc..Wherein fluid preservation is most easily promoted, most widely used.
Existing preservation liquid is mainly formalin-fixed liquid, and formalin solution is injected into the blood vessel of human body can be to prevent
The only corruption of corpse.Formalin specimen preserving liquid has good fixed function, this is because formaldehyde and sample protein
Functional group largely combines, and long-term placement further polymerization forms paraformaldehyde.This process is allowed in protein peptide interchain shape
At the crosslinking of appropriateness, can sterilize and the tissue morphology of sample.But it is with strong impulse, to human body
Harm be well-known, prior art prove formaldehyde be a kind of confirmation human carcinogen, kinds cancer can be induced, and can lead
Genetic lesions and mutation are caused, the harmfulness of formaldehyde is reduced, low toxicity, efficient preservation liquid is developed to replace formalin solution, improves
Working and learning environment ensures the health of teachers and students, is the problem of anatomy practitioner pays close attention to.
Summary of the invention
In order to solve the above-mentioned technical problem, an aspect of of the present present invention provides a kind of specimen preserving liquid, and component is as follows:
In some embodiments of the present invention, the component for saving liquid is as follows:
In some specific embodiments of the invention, the component for saving liquid is as follows:
In other specific embodiments of the invention, the component for saving liquid is as follows:
In other specific embodiment of the invention, the component for saving liquid is as follows:
In embodiments of the invention, the sample is animal tissue specimens.
In some embodiments of the present invention, the sample is preparation.
In some specific embodiments of the invention, the sample is human body specimen.
The second aspect of the present invention provides a kind of preparation method of specimen preserving liquid described described in first aspect present invention,
The following steps are included:
1) benzoic acid is dissolved in ethyl alcohol, forms solution A；
2) potassium nitrate, iron oxide and glycerol is soluble in water, form solution B；
3) solution A and solution B are mixed to get the specimen preserving liquid is arrived.
The third aspect of the present invention provides a kind of Saving specimen method, is protected using sample described in the first aspect of the present invention
Liquid storage is saved.
In some embodiments of the present invention, during preservation, alcohol is added constantly to supplement the volatilization of alcohol.
In embodiments of the invention, the sample is animal tissue specimens.
In some embodiments of the present invention, the sample is preparation.
In some specific embodiments of the invention, the sample is human body specimen.
Beneficial effects of the present invention
Preservation liquid of the invention is free of formaldehyde, and volatilization is slow, has no irritating odor, toxicity is very low, highly-safe, preservation effect
Good, long period preservation will not go mouldy, and can accomplish the original fixation of sample, not injury tissue structure, and can play good
Good color stabilizer effect, Saving specimen is complete, skin histology not blackening, and soft, has no irritating odor, sample slice
Observation, cell membrane save completely, and institutional framework is clear, and muscle band is high-visible.
Detailed description of the invention
Fig. 1 shows loss of liquid amount after preservation liquid and formalin-fixed liquid of the invention are placed 2 years.
Specific embodiment
In order to which the technical problems, technical solutions and beneficial effects solved by the present invention is more clearly understood, below in conjunction with
Embodiment, the present invention will be described in further detail.
Embodiment
Following example is used herein to demonstration the preferred embodiments of the invention.Those skilled in the art, it will be appreciated that under
State the technology disclosed in example represent inventor discovery can be used for implementing technology of the invention, therefore can be considered as implementation this
The preferred embodiment of invention.But those skilled in the art should be understood that specific reality disclosed herein according to this specification
Many modifications can be made by applying example, still can be obtained identical or similar as a result, rather than away from the spirit or scope of the present invention.
Unless otherwise defined, the term of all technologies as used herein and science, and the technology in fields of the present invention
Personnel institute is normally understood equivalent in meaning, and being disclosed reference and their materials of reference will all be incorporated.
Those skilled in the art will recognize or just will appreciate that by routine test many described here
Invention specific embodiment many equivalent technologies.These will equally be comprised in claims.
The preparation of the preservation liquid 1 of embodiment 1
It is prepared by following method and saves liquid 1:
1) 8g benzoic acid is dissolved in 120mL ethyl alcohol, forms solution A；
2) 17g potassium nitrate, 4g iron chloride and 120mL glycerol are dissolved in 700mL water, form solution B；
Solution A and solution B are mixed, add water to be supplemented to 1000mL to get preservation liquid 1 is arrived.
1 clear of preservation liquid provided in this embodiment, non-stimulated smell.
The preparation of the preservation liquid 2 of embodiment 2
It is prepared by following method and saves liquid 2:
1) 10g benzoic acid is dissolved in 150mL ethyl alcohol, forms solution A；
2) 15g potassium nitrate, 5g iron chloride and 100mL glycerol are dissolved in 700mL water, form solution B；
Solution A and solution B are mixed, add water to be supplemented to 1000mL to get preservation liquid 2 is arrived.
2 clear of preservation liquid provided in this embodiment, non-stimulated smell.
The preparation of the preservation liquid 3 of embodiment 3
It is prepared by following method and saves liquid 3:
1) 12g benzoic acid is dissolved in 160mL ethyl alcohol, forms solution A；
2) 13g potassium nitrate, 7g iron chloride and 90mL glycerol are dissolved in 700mL water, form solution B；
Solution A and solution B are mixed, add water to be supplemented to 1000mL to get preservation liquid 3 is arrived.
3 clear of preservation liquid provided in this embodiment, non-stimulated smell.
Embodiment 4 saves liquid status
Liquid 1, preservation liquid 2 will be saved respectively, save liquid 3 and formalin-fixed liquid (10% formaldehyde) placement 2 years.
After comparison is it is found that place 2 years, although each solution still keeps clear.But liquid 1 is saved, liquid 2 is saved and protects
Liquid storage 3 is still without any penetrating odor, and formalin-fixed liquid still has strong impulse smell.And after 2 years place,
The loss amount of formalin-fixed liquid is more than 10%, is significantly higher than and saves liquid 1, saves liquid 2 and save liquid 3 (as shown in Figure 1).
Embodiment 4 saves liquid to the preservation effect of human body specimen
In order to which more of the invention saves liquid and formalin-fixed liquid to the preservation effect of human body specimen, inventor's difference
Preservation liquid 1, preservation liquid 2, preservation liquid 3 and the formalin-fixed liquid (10% formaldehyde) provided using embodiment 1-3 is to human body
Sample is saved (annual summer adds some alcohol later, to supplement the alcohol vapored away).After saving 2 years, observation saves effect
Fruit, and light under the microscope its tectology structure change, it is as follows as the result is shown:
It can be seen that preservation liquid preservation effect of the invention is more preferable, wherein the preservation effect for saving liquid 2 is best.
All references mentioned in the present invention is incorporated herein by reference, independent just as each document
It is incorporated as with reference to such.In addition, it should also be understood that, after reading the above teachings of the present invention, those skilled in the art can
To make various changes or modifications to the present invention, such equivalent forms equally fall within model defined by the application the appended claims
It encloses.
Claims (10)
1. a kind of specimen preserving liquid, which is characterized in that component is as follows:
2. specimen preserving liquid according to claim 1, which is characterized in that component is as follows:
3. specimen preserving liquid according to claim 2, which is characterized in that component is as follows:
4. specimen preserving liquid according to claim 1 to 3, which is characterized in that the sample is animal tissue specimens.
5. specimen preserving liquid according to claim 1 to 3, which is characterized in that the sample is preparation.
6. specimen preserving liquid according to claim 5, which is characterized in that the sample is human body specimen.
7. the preparation method of any specimen preserving liquid of claim 1-3, which comprises the following steps:
1) benzoic acid is dissolved in ethyl alcohol, forms solution A；
2) potassium nitrate, iron oxide and glycerol is soluble in water, form solution B；
3) solution A and solution B are mixed to get the specimen preserving liquid is arrived.
8. a kind of Saving specimen method, which is characterized in that protected using any specimen preserving liquid of claim 1-3
It deposits.
9. Saving specimen method according to claim 8, which is characterized in that during preservation, constantly add alcohol with
Supplement the volatilization of alcohol.
10. according to any store method of claim 8-9, which is characterized in that the sample is human body specimen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811612730.1A CN109452261A (en) | 2018-12-27 | 2018-12-27 | A kind of specimen preserving liquid and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811612730.1A CN109452261A (en) | 2018-12-27 | 2018-12-27 | A kind of specimen preserving liquid and preparation method thereof |
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| Publication Number | Publication Date |
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| CN109452261A true CN109452261A (en) | 2019-03-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201811612730.1A Pending CN109452261A (en) | 2018-12-27 | 2018-12-27 | A kind of specimen preserving liquid and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113973804A (en) * | 2021-10-21 | 2022-01-28 | 武汉中纪生物科技有限公司 | Cervical cell preservation solution |
Citations (5)
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|---|---|---|---|---|
| CN1086658A (en) * | 1993-08-19 | 1994-05-18 | 周正国 | Antiseptic preserving liquid for dead body specimen |
| CN101438698A (en) * | 2008-12-17 | 2009-05-27 | 中南大学 | Anti-corrosive perfusate and preservative fluid of biological wet carcase |
| CN105360108A (en) * | 2015-12-16 | 2016-03-02 | 贵阳中医学院 | Specimen preservation fluid good in preservation effect and preparation method of specimen preservation fluid |
| CN105532637A (en) * | 2016-01-07 | 2016-05-04 | 别永信 | Human body teaching specimen preserving fluid and preparing method thereof |
| CN106359367A (en) * | 2016-08-30 | 2017-02-01 | 河南新大阳生物技术有限公司 | Formaldehyde-free specimen tissue preserving fluid and preparation method thereof |
-
2018
- 2018-12-27 CN CN201811612730.1A patent/CN109452261A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1086658A (en) * | 1993-08-19 | 1994-05-18 | 周正国 | Antiseptic preserving liquid for dead body specimen |
| CN101438698A (en) * | 2008-12-17 | 2009-05-27 | 中南大学 | Anti-corrosive perfusate and preservative fluid of biological wet carcase |
| CN105360108A (en) * | 2015-12-16 | 2016-03-02 | 贵阳中医学院 | Specimen preservation fluid good in preservation effect and preparation method of specimen preservation fluid |
| CN105532637A (en) * | 2016-01-07 | 2016-05-04 | 别永信 | Human body teaching specimen preserving fluid and preparing method thereof |
| CN106359367A (en) * | 2016-08-30 | 2017-02-01 | 河南新大阳生物技术有限公司 | Formaldehyde-free specimen tissue preserving fluid and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 中国人民解放军兽医大学药理教研室: "《新编兽医药物手册》", 31 December 1986 * |
| 张纯清 等: "标本无味保存液性能及配方", 《邯郸医学高等专科学校学报》 * |
| 张纯清 等: "自制无味保存液对解剖学肌标本12a保存效果的观察", 《现代中西医结合杂志》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113973804A (en) * | 2021-10-21 | 2022-01-28 | 武汉中纪生物科技有限公司 | Cervical cell preservation solution |
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Application publication date: 20190312 |