CN101473812B - Antiseptic fixing liquid composition for cadaver sample and preparation method thereof - Google Patents
Antiseptic fixing liquid composition for cadaver sample and preparation method thereof Download PDFInfo
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- CN101473812B CN101473812B CN2009100009912A CN200910000991A CN101473812B CN 101473812 B CN101473812 B CN 101473812B CN 2009100009912 A CN2009100009912 A CN 2009100009912A CN 200910000991 A CN200910000991 A CN 200910000991A CN 101473812 B CN101473812 B CN 101473812B
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Abstract
The invention discloses an antiseptic stationary liquid composition for corpse specimen, mainly comprising a metal complexing agent, a protein corsslinking agent, an osmoregulation agent and a food-grade antiseptic/mildew inhibitor and water; the metal complexing agent is ethylenediamine tetracetic acid disodium salt or ethylene glycol diethyl ether ethylenediamine tetracetic acid, the protein corsslinking agent is glyoxal, malonaldehyde, succinaldehyde or glutaraldehyde, the osmoregulation agent is a mixture, according to any proportion, of more than two substances selected from potassium nitrate, sodium nitrate, potassium chloride and sodium chloride, the food-grade antiseptic/mildew inhibitor is sodium dehydroacetate, sodium diacetate or potassium sorbate. The inventive composition is dedicated to antisepsis and fixing perfusion for animal or human corpses and has the advantages of strong antiseptic performance, good fixing performance and excellent safety without formaldehyde and toxicity. The invention also provides a method for preparing the antiseptic stationary liquid composition for corpse specimen.
Description
Technical field
The present invention relates to the fixedly perfusion of cadaver sample in a kind of medical teaching and the research work, anticorrosion, mildew-resistant.
Background technology
The human or animal after death, if tissue automatic soup-dissolving will soon take place without anticorrosion fixedly processing in corpse, corruption is disintegrated gradually.Sample is preserved and is generally carried out in two steps: be fed into corpse with fixer earlier and fix, will preserve with preserving liquid through the corpse after the anticorrosion fixedly processing.If corpse is fixing bad, then be difficult to play in the preservation process preserve moisture, conformal, antisepsis.
From the aqueous solution (formalin) that Blum in 1893 will contain formaldehyde be used for cadaver sample anticorrosion fixing since, be still the main component of most of anti-corrosion stationary liquids so far.Eerden shows that to 420 unit survey results formalin accounts for 97% as the fixer main component, and formalin is strong to the fixedly induration of tissue, advantages such as permeability is strong, price is low, sterilizing power is strong, favorable anti-corrosion effect.But facts have proved that its significant disadvantages is: behind the fixing sample substantially tissue is hardened and become fragile, be difficult for tractive, give to dissect and operate and detect and make troubles.Formaldehyde has the strong impulse smell, and contaminated environment causes student or staff can't examine sample, has directly influenced student and work effect, and serious harm anatomic teaching personnel and student's is healthy.Now, 95% above unit all uses the fixer fixed cadaver sample that contains formaldehyde.
Formalin is main component with alcohol, methyl alcohol, phenol then when not making main composition.But fix with phenol, the sample color is not good, bad smell is arranged, nerve endings is had infringement; Fix with alcohol, methyl alcohol, dehydration is too strong, and the sample shrinkage factor is big, and energy dissolved fat and lipoid; Fix with the salt concentrated solution, dehydration is too strong, and the sample tissue contracts is given to dissect to operate and brought difficulty.
The improvement of present anti-corrosion stationary liquid on prescription, be still with formaldehyde, phenol as the basic fixed agent, pluses and minuses according to each fixer, collocation by a certain percentage, sample is fixed, in the preservation process of sample, take certain methods to remove too much formaldehyde and phenol then, to reduce its toxicity.People such as Meng Jingwei have reported the fixer of doing with rare earth material, have fixed effect preferably, but rare earth material costs an arm and a leg, and are difficult to popularize.PrzemysLaw Majewski has reported that carrying out specimen anti-corrosion with ionic liquid fixes recently, and piece of tissue is placed in the ionic liquid and preserves, and is respond well, but it is only applicable to the organ cell, can not be used for substantially.Chinese patent CN101084746A discloses a kind of specimen anti-corrosion stationary liquid, form by organic acid, fatty alcohol, amine, sulfone, organic amine salt and water, be a kind of human body, animal, anticorrosion, fixing solution of plant tissue specimens of being used for, anticorrosion, fixed performance is better than formalin.But it is mainly used in fixing, the immunohistochemistry microsection manufacture of organ cell pathological section diagnosis in hospital pathology department, the Surgical Operating Room, also is only applicable to the organ cell and fixes, and the fixer penetration is poor, the cost height, is not suitable for substantially.
Summary of the invention
Technical problem to be solved by this invention provides a kind of anti-corrosion stationary liquid composition of the animal or human's of being specifically designed to body cadaver sample.This composition material meets anatomical requirement, can effectively solve embalming, mildew-resistant, fixing problem.The characteristics of this anti-corrosion stationary liquid are not contain formaldehyde, and it is strong to have an antiseptic effect, and it is fast fixedly to come into force, and safety non-toxic, raw material are easy to get, low price, and the sample shrinkage factor of being preserved is little, and color and luster is near characteristics such as condition of living organism.The present invention also provides this cadaver sample anti-corrosion stationary liquid preparation of compositions method.
For solving the problems of the technologies described above, the technical scheme that the present invention takes is: a kind of anti-corrosion stationary liquid composition that is used for cadaver sample, said composition comprises metal chelating agent, protein-crosslinking agent, Osmolyte regulator and food-grade Antisepticize and mildew preventive, and in 100 kilograms of described compositions, each composition weight is:
Metal chelating agent: 0.2～0.5kg,
Protein-crosslinking agent: 0.2～0.8kg,
Osmolyte regulator: 0.5～1.5kg,
Food-grade Antisepticize and mildew preventive: 0.3～1.0kg,
Surplus is a water;
Wherein, described metal chelating agent is disodium EDTA or ethylene glycol diethyl ether ethylenediamine tetraacetic acid (EDTA), and described protein-crosslinking agent is glyoxal, malonaldehyde, butanedial or glutaraldehyde; Described Osmolyte regulator is the mixture that the two or more material in potassium nitrate, sodium nitrate, potassium chloride, the sodium chloride mixes with arbitrary proportion; Described food-grade Antisepticize and mildew preventive is sodium dehydroacetate, sodium Diacetate or potassium sorbate.
The present invention also provides a kind of anti-corrosion stationary liquid preparation of compositions method that is used for cadaver sample, prepares 100 kilograms of described compositions and comprises the steps:
A. take by weighing metal chelating agent 0.2～0.5kg, add protein-crosslinking agent 0.2～0.8kg, add running water to 10 a kilogram normal temperature and pressure and stir;
B. add Osmolyte regulator 0.5～1.5kg in the mixture in the steps A, Antisepticize and mildew preventive 0.3～1.0kg adds running water to 100 kilogram, and the normal temperature and pressure stirring and dissolving gets final product.
The present invention makes enzyme deactivation, protein coagulation according to enzyme deactivation, the necessary condition of protein coagulation with complexing of metal ion agent (disodium EDTA, ethylene glycol diethyl ether ethylenediamine tetraacetic acid (EDTA)), food-grade Antisepticize and mildew preventive (sodium dehydroacetate, sodium Diacetate, potassium sorbate etc.), prevent tissue automatic soup-dissolving, reach sterilization, anticorrosion and fixing purpose.Utilizing the semipermeable membrane principle to add Osmolyte regulator (sylvite, sodium salt etc.) the interior body fluid of anticorrosion fixedly perfusion liquid and body such as is in to ooze environment, prevent sample oedema or overvulcanization.According to albumen, fat splitting and catabolite is these facts such as amino acid, nitrate, adds albumen, fat splitting inhibitor (nitrate etc.), and Profilin, fat splitting, degraded balance move, and sample is reached preserve moisture, conformal and fixing again.
The beneficial effect that the present invention can reach is:
1, the present composition does not contain formaldehyde, safety and low toxicity, and nonirritant, to environment and body harmless, chmice acute toxicity is irritated stomach dosage greater than 12, and 000mg/Kg, product are low toxicity.
2, combination group of the present invention in the embalming mildew-resistant is fixing, is led the city to metal chelating agent, food-grade Antisepticize and mildew preventive first Application for materials such as complexing of metal ion agent, food-grade Antisepticize and mildew preventive provide new application.
3, by materials such as protein-crosslinking agent, Osmolyte regulator, albumen, fat splitting inhibitor sample is fixed, sample does not go mouldy after fixing, without putrefaction, and anticorrosion anti-mold effect and formalin fixed are close; Sample does not shrink after fixing, color is natural, it is pliable and tough, moistening to organize, and guarantor's look, conformal effect are better than formalin fixed.
4, present composition preparation method is simple, raw material is easy to get, low price, and is easy to utilize.
Embodiment
Embodiment 1
A kind of impregnating fluid composition that is specifically designed to animal or human's body cadaver sample is made up of disodium EDTA, glyoxal, potassium nitrate, sodium chloride, sodium dehydroacetate.
Prepare 100 kilograms of said compositions, take by weighing each component by following weight:
Disodium EDTA: 0.3kg,
Glyoxal: 0.5kg,
Potassium nitrate: 0.2kg,
Sodium chloride: 0.5kg,
Sodium dehydroacetate: 0.5kg,
Add running water to 100 kilogram.
The anti-corrosion stationary liquid preparation of compositions process of this cadaver sample is:
Elder generation is disodium EDTA, the glyoxal of weight as above, adds running water to 10 a kilogram normal temperature and pressure to stir, and adds potassium nitrate, sodium chloride, sodium dehydroacetate again, adds to 100 kilograms with running water, stirs 1 hour, is finished product after leaving standstill.
Embodiment 2
A kind of anti-corrosion stationary liquid composition that is used for cadaver sample, it is by the ethylene glycol diethyl ether ethylenediamine tetraacetic acid (EDTA), and butanedial, potassium nitrate, sodium nitrate, sodium Diacetate are formed.
Prepare 100 kilograms of said compositions, take by weighing each component by following weight:
Ethylene glycol diethyl ether ethylenediamine tetraacetic acid (EDTA): 0.4kg,
Butanedial: 0.4kg,
Potassium nitrate: 0.5kg,
Sodium nitrate: 0.6kg,
Sodium Diacetate: 0.3kg,
Add running water to 100 kilogram.
The anti-corrosion stationary liquid preparation of compositions process of this cadaver sample is:
Elder generation is ethylene glycol diethyl ether ethylenediamine tetraacetic acid (EDTA), the butanedial of weight as above, adds the about 10 kilograms of normal temperature and pressures of running water to stir, and adds sodium nitrate, sodium nitrate, sodium Diacetate again, adds to 100 kilograms with running water, stirs 2.0 hours, is finished product after leaving standstill.
Embodiment 3
A kind of anti-corrosion stationary liquid composition that is used for cadaver sample, it is by disodium EDTA, glutaraldehyde, and potassium nitrate, sodium nitrate, potassium chloride, sodium dehydroacetate are formed.
Prepare 100 kilograms of said compositions, take by weighing each component by following weight:
Disodium EDTA: 0.5kg,
Glutaraldehyde: 0.8kg,
Potassium nitrate: 0.4kg,
Sodium nitrate: 0.3kg,
Potassium chloride: 0.6kg,
Sodium dehydroacetate: 0.8kg,
Add running water to 100 kilogram.
The preparation process of this cadaver sample impregnating fluid fixing composition is:
Elder generation is disodium EDTA, the glutaraldehyde of weight as above, add the about 10 kilograms of normal temperature and pressures of running water and stir, add potassium nitrate, sodium nitrate, potassium chloride, sodium dehydroacetate again, add to 100 kilograms with running water, stirred 1.0 hours, and be finished product after leaving standstill.
Embodiment 4
A kind of anti-corrosion stationary liquid composition that is used for cadaver sample, it is by ethylene glycol diethyl ether ethylenediamine tetraacetic acid (EDTA), glutaraldehyde, and sodium nitrate, sodium chloride, sodium Diacetate are formed.
Prepare 100 kilograms of said compositions, take by weighing each component by following weight:
Ethylene glycol diethyl ether ethylenediamine tetraacetic acid (EDTA): 0.2kg,
Glutaraldehyde: 0.2kg,
Sodium nitrate: 0.2kg,
Sodium chloride: 0.3kg,
Sodium Diacetate: 0.4kg,
Add running water to 100 kilogram.
This cadaver sample anti-corrosion stationary liquid preparation of compositions process is:
Elder generation is ethylene glycol diethyl ether ethylenediamine tetraacetic acid (EDTA), the glutaraldehyde of weight as above, adds the about 10 kilograms of normal temperature and pressures of running water to stir, and adds sodium nitrate, sodium chloride, sodium Diacetate again, adds to 100 kilograms with running water, stirs 1.5 o'clock, is finished product after leaving standstill.
Embodiment 5
A kind of composition that is used for the anti-corrosion stationary liquid of cadaver sample, it is made up of disodium EDTA, malonaldehyde, sodium nitrate, potassium nitrate, potassium chloride, sodium chloride, potassium sorbate.
Prepare 100 kilograms of said compositions, take by weighing each component by following weight:
Disodium EDTA: 0.3kg,
Malonaldehyde: 0.5kg,
Sodium nitrate: 0.4kg,
Potassium nitrate: 0.6kg,
Potassium chloride: 0.2kg,
Sodium chloride: 0.3kg,
Potassium sorbate: 1.0kg;
Add running water to 100 kilogram.
This cadaver sample anti-corrosion stationary liquid preparation of compositions process is:
Elder generation is disodium EDTA, the malonaldehyde of weight as above, add the about 10 kilograms of normal temperature and pressures of running water and stir, add sodium nitrate, potassium nitrate, potassium chloride, sodium chloride, potassium sorbate again, add to 100 kilograms with running water, stirred 1.5 hours, and be finished product after leaving standstill.
The anti-corrosion stationary liquid composition for preparing is carried out fixing use of routine to the fixing cadaver sample of need to get final product.
Collect corpse 8 tools, wherein male corpse 5 tools, women corpse 3 tools.Wherein 4 tools man's corpse and 2 tools woman corpse are fixed with the composition of the embodiment of the invention 5, and 1 tool man's corpse and 1 tool woman corpse are fixed with the anti-corrosion stationary liquid of formalin routine.Preserve and dissect after 6～12 months, observe anticorrosion mildew-resistant and fixed effect, comparing result is as shown in the table.
| Fixer | Antiseptic effect | Anti-mold effect | The sample color | Sample toughness | Sample elasticity | The joint is moving active | The internal organs shape | Color | Environmental pollution | Smell |
| Formalin | Good | Good | Deepening | Firmly | Difference | Difference | Shrinkage | Relatively poor | Seriously | Strongly |
| The present invention | Good | Good | Nature | Soft | Good | Better | Original state | Clear | No | Tasteless |
The above is preferred embodiment of the present invention only, is not to be used to limit protection scope of the present invention.
Claims (2)
1. an anti-corrosion stationary liquid composition that is used for cadaver sample is characterized in that, said composition is made up of metal chelating agent, protein-crosslinking agent, Osmolyte regulator and food-grade Antisepticize and mildew preventive and water, and in the described composition of 100kg, each composition weight is:
Metal chelating agent: 0.2～0.5kg,
Protein-crosslinking agent: 0.2～0.8kg,
Osmolyte regulator: 0.5～1.5kg,
Food-grade Antisepticize and mildew preventive: 0.3～1.0kg,
Surplus is a water;
Wherein, described metal chelating agent is disodium EDTA or ethylene glycol diethyl ether ethylenediamine tetraacetic acid (EDTA), and described protein-crosslinking agent is glyoxal, malonaldehyde, butanedial or glutaraldehyde;
Described Osmolyte regulator is the mixture that the two or more material in potassium nitrate, sodium nitrate, potassium chloride, the sodium chloride mixes with arbitrary proportion;
Described food-grade Antisepticize and mildew preventive is sodium dehydroacetate, sodium Diacetate or potassium sorbate.
2. a kind of anti-corrosion stationary liquid preparation of compositions method that is used for cadaver sample according to claim 1 is characterized in that, the described composition of preparation 100kg comprises the steps:
A. take by weighing metal chelating agent 0.2～0.5kg, add protein-crosslinking agent 0.2～0.8kg, add running water to 10kg, normal temperature and pressure stirs;
B. add Osmolyte regulator 0.5～1.5kg in the mixture in the steps A, Antisepticize and mildew preventive 0.3～1.0kg adds running water to 100kg, and the normal temperature and pressure stirring and dissolving gets product.
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| CN2009100009912A CN101473812B (en) | 2009-01-24 | 2009-01-24 | Antiseptic fixing liquid composition for cadaver sample and preparation method thereof |
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| CN101984803B (en) * | 2010-09-26 | 2013-01-30 | 无锡市江原实业技贸总公司 | Specimen preserving, fixing and accelerating agent |
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| ITUB20160829A1 (en) * | 2016-02-18 | 2017-08-18 | Addax Biosciences S R L | FIXATIVE FOR HISTOLOGICAL PREPARATIONS INCLUDING ACLIXY GLIOSSAL |
| CN105842033A (en) * | 2016-05-13 | 2016-08-10 | 北京九州柏林生物科技有限公司 | Formaldehyde-free tissue specimen stationary liquid |
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| CN109566599A (en) * | 2018-12-25 | 2019-04-05 | 南京健邦锦源医疗仪器有限公司 | A kind of exfoliated cell preservative fluid |
| CN110880268B (en) * | 2019-11-12 | 2021-12-28 | 广东省生态环境技术研究所 | Method for preparing earthworm specimen |
| CN111226905A (en) * | 2020-03-25 | 2020-06-05 | 张家港市德仁科教仪器设备有限公司 | Novel environment-friendly specimen fixing composition, preparation method thereof and fixing liquid containing composition |
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