CN101496511A - Antisepsis composition for remains as well as preparation method and application - Google Patents

Antisepsis composition for remains as well as preparation method and application Download PDF

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CN101496511A
CN101496511A CNA2008100333061A CN200810033306A CN101496511A CN 101496511 A CN101496511 A CN 101496511A CN A2008100333061 A CNA2008100333061 A CN A2008100333061A CN 200810033306 A CN200810033306 A CN 200810033306A CN 101496511 A CN101496511 A CN 101496511A
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composition
methyl
remains
alcohol
gentle repose
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任祖翰
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Abstract

The invention relates to a remains decay-preventing composition, a preparation method and application thereof. The remains decay-preventing composition comprises the following components in proportion: 0.1 to 5 volume portions of isothiazolinone compounds, 5 to 30 volume portions of dibastic alcohol, 3 to 30 volume portions of glycerol, 5 to 30 volume portions of alcohol, 5 to 50 volume portions of hexanol, 0 to 15 weight portions of nipagin esters, 1 to 15 volume portions of dimethyl sulfoxide, 0 to 2 weight portions of ethylenediamine tetraacetic acid disodium salt, 0 to 10 volume portions of acetic acid, 0 to 15 weight portions of urotropine, 1 to 10 volume portions of plant extract and 0 to 15 volume portions of purified water. By pouring preservative into aortas and injecting the preservative into abdominal cavities, thoracic cavities, pharyngeal cavities and cranial cavities, the composition plays a rapid effective role in preventing remains decay. The decay-preventing composition has the advantages of broad-spectrum antibacterial property, fast action, good compatibility with enzyme and low toxicity, and is particularly applicable to the decay prevention of the remains in humid and other environments.

Description

A kind of Gentle Repose composition and preparation method and application
Technical field
The present invention relates to Gentle Repose technology in the field of funeral and interment, be specifically related to a kind of Gentle Repose composition and preparation method and application.
Background technology
Chinese patent ZL01132050.8 discloses a kind of preservative and preparation method; Chinese patent application CN200410053606.8 discloses a kind of composition and preparation method and application that is used for the short-term Gentle Repose, and CN200410066612.7 discloses a kind of composition and preparation method and application that is used for long-term Gentle Repose; Injection Gentle Repose method that Chinese patent ZL02137225.X discloses a kind of " four chambeies ".Disclosed preservative and aseptic technic are carried out preservative treatment from different angles to remains respectively in above-mentioned patent or the patent application, to be fit to the requirement of various customs, natural environment and appointed condition, have reached good antiseptic effect.
Peroxide has all been adopted in the Gentle Repose agent that foregoing preservatives and prior art adopted, although peroxide has antisepsis preferably, but it must have gas to produce simultaneously at corrosion-resistant, often causes the remains dilatancy, increases mental anguish to the family members of the deceased.In addition, the foregoing preservatives antiseptic effect is desirable not enough in a humid environment.The inventor is in order to improve the performance of anticorrosive composite; to adapt to the requirement for anticorrosion in varying environment and area; through groping repeatedly, make up successfully a kind of novel Gentle Repose agent of non-peroxide, meet the protection of environmental protection, Gentle Repose, requirement safe and convenient to use.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of can guarantee remains integrity, compliance with environmental protection requirements better, can adapt to the all-environment novel Gentle Repose composition that does not contain peroxide.
The novel Gentle Repose composition of the present invention is made up of following ratio component: 0.1-5v part isothiazolinone compounds, 5-30v part dihydroxylic alcohols, 3-30v part glycerine, 5-30v part alcohol, 5-50v part n-hexyl alcohol, 0-15w part nipagin esters, 1-15v part methyl-sulfoxide, 0-2w part disodium edta, 0-10v part acetate, 0-15w part Wu Luotuoping, 1-10v part plant extracts and 0-15v part pure water are formed, and also can add an amount of spices in case of necessity.Wherein v represents parts by volume, and w represents weight portion.
Isothiazolinone compounds of the present invention is preferably from 2-methyl-4-isothiazoline-3-ketone, 2-methyl-3 (2H)-isothiazolone, 2-methyl-3 (H)-isothiazolone, 1.2-benzisothiazole-3-ketone and 5-chlorine-2-methyl-4-isothiazoline-3-ketone; Described dihydroxylic alcohols is preferably from ethylene glycol, propane diols or their mixture.
Plant extracts of the present invention is to be the extract that raw material obtains behind alcohol extract with Chinese olive, rhizoma zingiberis, anise and reed rhizome, and the preferred weight ratio of each raw material is a Chinese olive: rhizoma zingiberis: anise: reed rhizome=1-2:1-3:0.5-1:2-4.
The present invention requires according to the difference of anticorrosion object and the needs of anticorrosion operation, optimizes two kinds of prescriptions that not only can use separately but also can be used, they consist of:
A prescription: 0.1-2v part isothiazolinone compounds, 5-30v part dihydroxylic alcohols, 3-20v part glycerine, 5-20v part alcohol, 5-30v part n-hexyl alcohol, 1-15v part methyl-sulfoxide, 0.1-2w part disodium edta, 1-10v part acetate, 1-15w part Wu Luotuoping, 1-10v part plant extracts and 1-15v part pure water.
B prescription: 2-5v part isothiazolinone compounds, 5-20v part dihydroxylic alcohols, 3-30v part glycerine, 5-30v part alcohol, 5-50v part n-hexyl alcohol, 1-15w part nipagin esters, 1-15v part methyl-sulfoxide, 1-10v part plant extracts.
Another technical problem to be solved by this invention is to disclose the preparation method of above-mentioned anticorrosive composite.
1) in proportion plant Chinese olive, rhizoma zingiberis, anise and reed rhizome are smashed to pieces, got 100 grams and be immersed in the container that 200 ml ethanols are housed, 60 ℃ of water-baths were heated 6 hours, leached soup, and are standby after the activated carbon filtration decolouring;
2) standby after in proportion the gentle disodium edta of Wu Luotuo being dissolved in the pure water;
3) will stir evenly in nipagin esters and isothiazolinone compounds adding dihydroxylic alcohols and the alcohol in proportion, add glycerine, acetate and n-hexyl alcohol then successively, a kind of article of every adding stirred 20-40 minutes, leave standstill and moments later add methyl-sulfoxide, continue to stir, add above-mentioned steps 1 in proportion) and 2) reserve liquid that makes, stir, leave standstill 24 hours promptly.
A technical problem more to be solved by this invention is to disclose the application of above-mentioned anticorrosive composite in Gentle Repose.
The active component of Gentle Repose composition of the present invention mainly is nipagin esters and isothiazolinone compounds, and these two kinds of compositions suppress the growth of cell and promote that apoptotic principle is identical.When they with after cell membrane contacts a few minutes, can be penetrated in the film immediately and suppress cytoactive.Pyruvate dehydrogenase, α-ketoglutaric dehydrogenase, succinate dehydrogenase and nadh dehydrogenase are the key enzymes that promotes the cellular metabolism circulation, and anticorrosive composite of the present invention is by acting on this four kinds of different enzymes, thereby cell growth inhibiting metabolism and macromolecular synthetic, and cause that the intracellular energy level descends rapidly.Because the collapse of energy system, cell can not synthesize the needed compound of daily metabolism, finally reaches the corrosion-resistant purpose.
All bacteriums and fungi all have the part circulation, and the anticorrosive composite scope of application of the present invention is very extensive, and have the characteristics of many targets action site, therefore greatly reduce variation microorganism drug resistance.Along with circulation is blocked, the ability of bioactivators such as cell produce power and synzyme descends rapidly, makes antiseptic effect more remarkable.
After Wu Luotuoping enters remains, under sour environment, can solidify the albumen of bacterium, directly kill bacteria.Simultaneously, it has solidified the connective tissue between epidermis and the corium, forms barrier one, stops corrupt gas and liquid to escape, and has blocked corrupt development, effectively prevents exfoliation, reaches the effect of deodorizing indirectly.Because the epidermis drying also can reduce intrusion of extraneous bacterium and the growth of mould.
The present invention has optimized two prescriptions of A, B according to the difference requirement of anticorrosion object and the needs of anticorrosion operation.Wherein the A prescription is applicable to the puncture aortic perfusion of remains, plays the effect of whole body corrosion-resistant.The B prescription is applicable to and is expelled to the abdominal cavity simultaneously, the thoracic cavity, and pharyngeal cavity and cranial cavity (being called for short four chambeies) play the antisepsis to the remains torso interior.A, B prescription both can use separately, can unite use again.When A, B prescription is united use, antiseptic effect the best, remains can at room temperature be preserved 30 days at least.
Generally the consumption of A prescription depends on the fate of remains preservation and the degree of remains corruption.The fate that A prescription can be preserved according to every tool remains and the degree of remains corruption decide inventor's suggestion: remains need preserve 15 days with about interior A prescription perfusion 2000ml, preserve about 20 days A prescription perfusion 2500ml, preserve about 25 days A prescription perfusion 3000ml, preserve that the A prescription pours into about 3500ml more than 25 days.As the corresponding raising 500-1000ml of remains corrupt obviously A prescription groundwater increment.
It is anticorrosion that A prescription adopts puncture arch of aorta perfusion impregnating fluid to reach whole body, abandoned up to now with the conventional method of the method for operating at intra-arterial perfusion impregnating fluid, and it goes without doing otch had both reached the most effective whole body antiseptic effect, can be accepted by the family of the deceased again.
The concrete operation method of the bright puncture arch of aorta perfusion of we impregnating fluid is:
1, the topography of the arch of aorta and surface projection:
Topography: the arch of aorta is extended by aorta ascendens, in the back of breastbone, and the front of tracheae, a left side is a pulmonary artery, the right side is a superior vena cava;
Surface projection: the breastbone median line between 2,3 ribs, is no more than the left border of breastbone; Aortal anatomical position is fixed, and is not subjected to the influence of factors such as age, sex, the bodily form, body weight, disease;
2, entry point: the bottom of suprasternal fossa;
3, needle angle: be 25-45 degree with skin;
4, the degree of depth: 10-12 centimetres;
5, pin sense: have resistance sense behind 6-7 centimetres of the inserting needles, inserting needle has the sensation of " pouncing on now " again, after the sense that falls through is arranged;
6, the successful observation of perfusion:
1) outside the perfusion arch of aorta, perfusion liquid can be advanced not take out;
2) be filled in the blood vessel, distinguish artery or vein, see the color and the amount of blood;
3) be filled in the tracheae, except that the sensation that when puncture arranged, also be to inject to extract out fewly more, and mouth and nose have liquid to overflow;
7, rate of flooding: the speed of perfusion was advisable with 100ml/ minute.
Can be expelled to the abdominal cavity by the B prescription separately for fresh remains, the thoracic cavity, pharyngeal cavity and cranial cavity (being called for short four chambeies) are preserved in 3 days it; When B prescription and A prescription use in conjunction, remains can be preserved more than 30 days, and all can carry out good anticorrosion preservation to any corrupt remains.
The conventional amount used of B prescription is: the 120-200 milliliter is injected to cavum peritoneale and intraperitoneal through navel; Respectively inject the 40-60 milliliter to left and right sides pleural cavity and thoracic cavity through left and right sides arcus costarum lower edge mid point again; Per os or nose inject the 8-12 milliliter to pharyngeal cavity then; Last intranasal puncture sieve skeleton injection 10-15 milliliter is injected the chest abdominal massaging that finishes to cranial cavity.
Anticorrosive composite A prescription of the present invention makes impregnating fluid be distributed to the remains whole body fast, equably by aortal perfusion, and blocking-up is corrupt, the control self-dissolving.Corrupt gas that produces of remains and liquid leak to epidermis and form tension force gas blister and blister, and the swelling of very easily peeling distortion blackening is smelly.The A prescription can suppress shallow table bacterium effectively, and fixing being connected of corium and epidermis, prevents the loose or dislocation of epidermis.The B prescription can play local directly the immersion and infiltration to internal organ and trunk tissue by injecting remains " four chambeies " (abdominal cavity, thoracic cavity, pharyngeal cavity and cranial cavity) are interior, and it is corrupt with blocking-up in time to suppress self-dissolving.When the B prescription is united use with the A prescription, replenished the time difference of anticorrosive composite A prescription effect.The use in conjunction of anticorrosive composite A, B prescription has strengthened the antiseptic effect of remains, can also deodorizing and softening tissue, and the complete form of protection remains.
With the Gentle Repose composition of the present invention bacteriostatic test of being correlated with, concrete outcome is as follows:
1. for the examination bacterial classification:
Distillery yeast, aspergillus niger, aspergillus flavus, variable color aspergillus, Penicillium citrinum, paecilomyces varioti, Chaetomium globosum, Trichoderma viride, cladosporium herbarum, staphylococcus aureus, streptococcus, hay bacillus, Escherichia coli, fluorescent pseudomonas, bacillus megaterium.
2. testing conditions:
Temperature: bacterium is 36 ± 1 ℃, and mould and saccharomycete are 28 ± 1 ℃
Time: bacterium and saccharomycete are 24 hours, and mould is 72 hours
3. detection method: dull and stereotyped inhibition zone method
4. test sample:
Anticorrosive composite A prescription of the present invention
5, control group:
Test strain is adding preservative agent not, own control.
6. testing result:
Table 1 fungistatic effect testing result
Figure A200810033306D00091
(in the table +++for the full visual field growth of perusal culture plate for the test bacterium)
Above-mentioned test shows that anticorrosive composite of the present invention has broad spectrum antibacterial, and speed of action is fast, with enzyme favorable compatibility is arranged, and can not influence the correlated response with enzyme antibody.Through using the A prescription that 200 routine remains are carried out preservative treatment (wherein 10 examples are used in humidity 70-93% environment at 30-35 degree Celsius), remains complete form symmetry in 3-10 days is seemingly slept, the limbs softness, and no stink, the chest belly does not have bulge.
Gentle Repose composition of the present invention all has bactericidal action in PH2-9 scope, it is constant substantially that this product is placed on room temperature next year active component.Do not produce gas in anticorrosion process, toxicity is low.Be specially adapted to the Gentle Repose under the adverse circumstances such as humidity.
Embodiment
The preparation of embodiment 1 A prescription
2-methyl-3 (2H)-isothiazolone 20ml
Ethylene glycol 200ml
Glycerine 100ml
N-hexyl alcohol 200ml
Wu Luotuoping 40g
Alcohol 120ml
Disodium edta 20g
Acetate 50ml
Methyl-sulfoxide 50ml
Chinese olive: rhizoma zingiberis: anise: reed rhizome (1:1:1:2) 50ml
Pure water 150ml
Rue spices a little
1) by proportioning plant Chinese olive, rhizoma zingiberis, anise and reed rhizome are smashed to pieces, got 100 grams and be immersed in the container that 200 ml ethanols are housed, container is put in 60 ℃ of water-baths heated 6 hours, leach soup, standby after the activated carbon filtration decolouring;
2) standby after being dissolved in the gentle disodium edta of Wu Luotuo in the pure water by proportioning;
3) will stir evenly in the isothiazolinone compounds adding alcohol by proportioning, add dihydroxylic alcohols, glycerine, acetate and n-hexyl alcohol then successively, a kind of article of every adding stirred 20-40 minutes, leave standstill and moments later add methyl-sulfoxide, continue to stir, add above-mentioned steps 1 in proportion) and 2) reserve liquid that makes, stir, rue spices a little, leave standstill 24 hours promptly.
The preparation of embodiment 2 B prescription
2-methyl-3 (2H)-isothiazolone 40ml
Ethylene glycol 100ml
Glycerine 100ml
Alcohol 100ml
N-hexyl alcohol 360ml
Nipagin esters 140g
Methyl-sulfoxide 110ml
Chinese olive: rhizoma zingiberis: anise: reed rhizome (2:1:1:3) 50ml
1) by proportioning plant Chinese olive, rhizoma zingiberis, anise and reed rhizome are smashed to pieces, got 100 grams and be immersed in the container that 200 ml ethanols are housed, container is placed in 60 ℃ of water-baths heated 6 hours, leach soup, standby after the activated carbon filtration decolouring;
2) will stir evenly in nipagin esters and the isothiazolinone compounds adding alcohol by proportioning, add dihydroxylic alcohols, glycerine and n-hexyl alcohol then successively, a kind of article of every adding stirred 20-40 minutes, leave standstill and moments later add methyl-sulfoxide, continue to stir, add above-mentioned steps 1 in proportion) reserve liquid that makes, stir, leave standstill 24 hours promptly.
Embodiment 3 A formulation
2-methyl-4-isothiazoline-3-ketone 15ml
Propane diols 100ml
Glycerine 50ml
N-hexyl alcohol 214ml
Wu Luotuoping 150g
Alcohol 200ml
Methyl-sulfoxide 100ml
Disodium edta 1g
Acetate 100ml
Chinese olive: rhizoma zingiberis: anise: reed rhizome (1.5:1:0.5:3) 70ml
Pure water 145ml
Rue spices a little
The preparation method is with embodiment 1
The preparation of embodiment 4 B prescription
2-methyl-4-isothiazoline-3-ketone 50ml
Propane diols 150ml
Glycerine 150ml
Alcohol 300ml
N-hexyl alcohol 100ml
Nipagin esters 150g
Methyl-sulfoxide 50ml
Chinese olive: rhizoma zingiberis: anise: reed rhizome (2:2:0.5:4) 50ml
The preparation method is with embodiment 2
The preparation of embodiment 5 A prescription
1.2-benzisothiazole ketone-3-ketone 5ml
Ethylene glycol 50ml
Propane diols 80ml
Glycerine 100ml
N-hexyl alcohol 280ml
Wu Luotuoping 100g
Alcohol 70ml
Methyl-sulfoxide 70ml
Disodium edta 1.5g
Acetate 20ml
Chinese olive: rhizoma zingiberis: anise: reed rhizome (2:3:1:2) 100ml
Pure water 123.5ml
Rue spices a little
The preparation method is with embodiment 1
The preparation of embodiment 6 B prescription
1.2-benzisothiazole ketone-3-ketone 20ml
Ethylene glycol 100ml
Propane diols 100ml
Glycerine 250ml
Alcohol 80ml
N-hexyl alcohol 200ml
Nipagin esters 50g
Methyl-sulfoxide 150ml
Chinese olive: rhizoma zingiberis: anise: reed rhizome (1:1:1:1) 50ml
The preparation method is with embodiment 2
Embodiment 7
Get the anticorrosive composite B liquid that embodiment 2 makes.After the remains shower, whole body is dried.Earlier 180 milliliters of the B parts of anticorrosive composite are injected to cavum peritoneale and intraperitoneal through navel; Respectively inject 50 milliliters to left and right sides pleural cavity and thoracic cavity through left and right sides arcus costarum lower edge mid point again; Per os or nose inject 10 milliliters to pharyngeal cavity then; Last intranasal puncture sieve skeleton is injected 12 milliliters to cranial cavity, injects the chest abdominal massaging that finishes.Anticorrosion result is as follows:
Figure A200810033306D00131
Figure A200810033306D00141
Embodiment 8
Get anticorrosive composite A, B liquid that embodiment 3,4 makes respectively.After the remains shower, whole body is dried.Through the suprasternal fossa puncture arch of aorta, pour into anticorrosive composite A liquid 3000ml earlier; 180 milliliters of the B liquid of anticorrosive composite are injected to cavum peritoneale and intraperitoneal through navel, respectively inject 50 milliliters to left and right sides pleural cavity and thoracic cavity through left and right sides arcus costarum lower edge mid point again, per os or nose inject 10 milliliters to pharyngeal cavity then, last intranasal puncture sieve skeleton is injected 12 milliliters to cranial cavity, injects the chest abdominal massaging that finishes.Anticorrosion result is as follows:
Figure A200810033306D00142
Figure A200810033306D00151
Figure A200810033306D00161
Embodiment 9
Get the anticorrosive composite A liquid that embodiment 1 makes.After the remains shower, whole body is dried.Through the suprasternal fossa puncture arch of aorta, pour into anticorrosive composite A liquid 2500ml earlier.Anticorrosion result is as follows:
Figure A200810033306D00162
Figure A200810033306D00171

Claims (10)

1, a kind of Gentle Repose composition is characterized in that said composition is made up of following ratio component: 0.1-5v part isothiazolinone compounds, 5-30v part dihydroxylic alcohols, 3-30v part glycerine, 5-30v part alcohol, 5-50v part n-hexyl alcohol, 0-15w part nipagin esters, 1-15v part methyl-sulfoxide, 0-2w part disodium edta, 0-10v part acetate, 0-15w part Wu Luotuoping, 1-10v part plant extracts and 0-15v part pure water.
2, Gentle Repose composition according to claim 1 is characterized in that said composition is made up of following ratio component: 0.10-2v part isothiazolinone compounds, 5-30v part dihydroxylic alcohols, 3-20v part glycerine, 5-20v part alcohol, 5-30v part n-hexyl alcohol, 1-15v part methyl-sulfoxide, 0.1-2w part disodium edta, 1-10v part acetate, 1-15w part Wu Luotuoping, 1-10v part plant extracts and 1-15v part pure water.
3, Gentle Repose composition according to claim 1 is characterized in that said composition is made up of following ratio component: 2-5v part isothiazolinone compounds, 5-20v part dihydroxylic alcohols, 3-30v part glycerine, 5-30v part alcohol, 5-50v part n-hexyl alcohol, 1-15w part nipagin esters, 1-15v part methyl-sulfoxide and 1-10v part plant extracts.
4,, it is characterized in that wherein said isothiazolinone compounds is 2-methyl-4-isothiazoline-3-ketone, 2-methyl-3 (2H)-isothiazolone, 2-methyl-3 (H)-isothiazolone, 1.2-benzisothiazole-3-ketone or 5-chlorine-2-methyl-4-isothiazoline-3-ketone according to each described Gentle Repose composition of claim 1 to 3.
5, according to each described Gentle Repose composition of claim 1 to 3, it is characterized in that wherein said dihydroxylic alcohols is selected from ethylene glycol, propane diols or their mixture.
6, according to each described Gentle Repose composition of claim 1 to 3, the weight ratio that it is characterized in that wherein said plant extracts is a Chinese olive: rhizoma zingiberis: anise: reed rhizome=1-2:1-3:0.5-1:2-4.
7, the described Gentle Repose method for compositions of a kind of preparation claim 1 is characterized in that this method is:
1) in proportion plant Chinese olive, rhizoma zingiberis, anise and reed rhizome are smashed to pieces, got 100 grams and be immersed in the container that 200 ml ethanols are housed, container is placed in 60 ℃ of water-baths heated 6 hours, leach soup, standby after the activated carbon filtration decolouring;
2) standby after being dissolved in the gentle disodium edta of Wu Luotuo in the pure water by proportioning;
3) will stir evenly in nipagin esters and the isothiazolinone compounds adding alcohol by proportioning, add dihydroxylic alcohols, glycerine and n-hexyl alcohol then successively, a kind of article of every adding stirred 20-40 minutes, leave standstill and moments later add methyl-sulfoxide, continue to stir, add above-mentioned steps 1 in proportion) and 2) reserve liquid that makes, stir, leave standstill 24 hours promptly.
8, the application of the described Gentle Repose composition of claim 1 in the agent of preparation Gentle Repose.
9, adopt the described Gentle Repose composition of claim 2 to carry out the method that Gentle Repose is handled, it is characterized in that this method adopts puncture arch of aorta perfusion impregnating fluid, the speed of perfusion is 100ml/ minute.
10, adopt the described Gentle Repose composition of claim 3 to carry out the method that Gentle Repose is handled, it is characterized in that this method is expelled to the abdominal cavity of remains with impregnating fluid, the thoracic cavity is in pharyngeal cavity and the cranial cavity.
CNA2008100333061A 2008-01-31 2008-01-31 Antisepsis composition for remains as well as preparation method and application Pending CN101496511A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101878755A (en) * 2010-07-06 2010-11-10 苏州博克精细化学品有限公司 Shampoo for body preservation
CN104521942A (en) * 2014-12-24 2015-04-22 博兴县殡仪馆 Body antiseptic agent
CN104798768A (en) * 2015-04-29 2015-07-29 合肥金域医学检验所有限公司 Preservation and treatment solution of in-vitro specimen for medical research
CN105010303A (en) * 2015-07-10 2015-11-04 毕爱胜 Corruption remains restoring method
CN106359367A (en) * 2016-08-30 2017-02-01 河南新大阳生物技术有限公司 Formaldehyde-free specimen tissue preserving fluid and preparation method thereof

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101878755A (en) * 2010-07-06 2010-11-10 苏州博克精细化学品有限公司 Shampoo for body preservation
CN104521942A (en) * 2014-12-24 2015-04-22 博兴县殡仪馆 Body antiseptic agent
CN104798768A (en) * 2015-04-29 2015-07-29 合肥金域医学检验所有限公司 Preservation and treatment solution of in-vitro specimen for medical research
CN105010303A (en) * 2015-07-10 2015-11-04 毕爱胜 Corruption remains restoring method
CN106359367A (en) * 2016-08-30 2017-02-01 河南新大阳生物技术有限公司 Formaldehyde-free specimen tissue preserving fluid and preparation method thereof

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