CN104798768A - Preservation and treatment solution of in-vitro specimen for medical research - Google Patents
Preservation and treatment solution of in-vitro specimen for medical research Download PDFInfo
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- CN104798768A CN104798768A CN201510224390.5A CN201510224390A CN104798768A CN 104798768 A CN104798768 A CN 104798768A CN 201510224390 A CN201510224390 A CN 201510224390A CN 104798768 A CN104798768 A CN 104798768A
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Abstract
The invention provides preservation and treatment solution of an in-vitro specimen for medical researches and relates to the technical field of treatment and preservation of specimens for medical researches. The preservation and treatment solution is prepared by using the following raw materials in parts by mass: 7 parts of boric acid, 13 parts of galangal, 10 parts of honeysuckle flower, 4 parts of common selfheal fruit-spike, 5 parts of red-rooted salvia root, 8 parts of wild chrysanthemum flower, 12 parts of corns officinalis, 15 parts of jujube and 15 parts of liquorice. According to actual use results, the preservation and treatment solution for histopathological tissue specimens can replace formalin for use. Tissue forms are kept well, the tissues are fully dyed, the microscopic cell structure is clear, the structure is complete and the requirements on thepreservation and treatment of the histopathological tissue specimens can be fully satisfied.
Description
Technical field
The present invention relates to process and the Techniques of preserving field of medical research sample, particularly relate to a kind of specimens preserving liquid of the in vitro sample for medical research.
Background technology
Histopathology be researching human body ill time each system organ's histological change a pathology subdiscipline.Its main research means be in vitro tissue is carried out after treatment cut into slices, dye after carry out observation by light microscope, diagnose the illness by observed result.Be not difficult to find out thus how to handle prerequisite and key that in vitro tissue sample is diagnosis and research well.After pathology sample is in vitro, because the change of microenvironment is by autolyze and (or) corruption, make its structural deterioration, simultaneously on microcosmic, cell degradation, self-dissolving.The object of process sample, state before making Pathologic specimen as far as possible keep it in vitro by various method exactly.More crucially, due to needs microscopic examination, therefore, Histopathology not only require in vitro sample in macroscopical (as color and luster, volume, soft durometer etc.) as far as possible close to state before in vitro, more requiring will as far as possible close to condition of living organism in the microcosmic of cellular level.
At present, formalin is the Histopathology sample processing reagent be most widely used.But because of its shortcoming such as high toxicity, high carcinogenic, damage the health of user always, high risks be also result in environment.
Therefore, search out that result of use is good, the Histopathology specimens preserving reagent of low toxicity, low irritant has very large academic significance and realistic meaning.
In fact, use Chinese herbal medicine as the method for preserving tissue or corpse, just occurred as far back as B.C. and applied.The pungent preservation and the preservation of unearthed Qing Dynasty noble female corpse etc. chasing after mummy in the Mawangdui, Han dynasty that Pharaoh's remains are preserved, China is unearthed as ancient Egypt, employs the Techniques of preserving of Chinese herbal medicine or even Chinese herb compound all in a large number.Modern Literature and scientific achievement also have multiple using the application of Chinese herbal medicine as preservative, achieve good result of use.
Though the present invention applies Chinese herbal medicine too as active ingredient, have basic different from above-mentioned prior art.
Medicinal herb components is mainly used as preservative by above-mentioned techniques and methods.Preservative is used to the medicament of inhibiting substances corruption, Main Function is the suppression continued the microbial growth taking corrupt substance as metabolism substrate, its objective is and macroscopically make the without putrefaction of corpse equal samples, never degenerate, keep the global index such as color and luster, soft durometer simultaneously.And the present invention is used to preserve in vitro sample and process, object not only to keep sample macroscopically as far as possible consistent with animation, the more important thing is, to make on microcosmic cell keep as far as possible in vitro before Morphology and structure, as organelle to preserve, nuclear fine structure (chromosome etc.) is complete, large contraction or expansion etc. does not occur cells intact.Therefore, the target that the present invention will realize and above-mentioned prior art have basic different, and the difficulty of realization is also larger.
Summary of the invention
For solving the problems of the technologies described above, the object of the present invention is to provide a kind of specimens preserving liquid of the in vitro sample for medical research.
Technical problem to be solved by this invention realizes by the following technical solutions:
For a specimens preserving liquid for the in vitro sample of medical research, it is characterized in that: be prepared from by the raw material of following mass fraction:
Boric acid 7 parts, galingal 13 parts, honeysuckle 10 parts, selfheal 4 parts, the red sage root 5 parts, mother chrysanthemum 8 parts, mountain cornel 12 parts, 15 parts, date, 15 parts, Radix Glycyrrhizae.
A kind of preparation method of specimens preserving liquid of the in vitro sample for medical research, it is characterized in that: take galingal 13 parts, honeysuckle 10 parts, selfheal 4 parts, the red sage root 5 parts, mother chrysanthemum 8 parts, mountain cornel 12 parts, 15 parts, date, 15 parts, Radix Glycyrrhizae, the soak by water of 500 parts is added after cleaning, during decoction liquor evaporation and concentration to 200 part, filter residue, gets decoction liquor, after decoction liquor cooling, add boric acid 7 parts, be uniformly mixed and obtain the specimens preserving liquid of in vitro sample.
The invention has the beneficial effects as follows:
Practical using result shows, this histopathology sample preservation and treating fluid can replace formalin to use.Tissue morphology keeps good, and fully, under mirror, cell structure is clear, structural integrity, can meet the specimens preserving requirement of Histopathology to sample completely in dyeing.
This product low toxicity, low stimulation, greatly can reduce the infringement to user's health and the harm to environment.
Embodiment
The technological means realized to make the present invention, creation characteristic, reaching object and effect is easy to understand, below in conjunction with specific embodiment, set forth the present invention further, but following embodiment being only the preferred embodiments of the present invention, and not all.Based on the embodiment in embodiment, those skilled in the art under the prerequisite not making creative work obtain other embodiment, all belong to protection scope of the present invention.
For a specimens preserving liquid for the in vitro sample of medical research, raw material comprises:
Boric acid 7g, galingal 13g, honeysuckle 10g, selfheal 4g, red sage root 5g, mother chrysanthemum 8g, mountain cornel 12g, date 15g, Radix Glycyrrhizae 15g.
Preparation method: take galingal 13g, honeysuckle 10g, selfheal 4g, red sage root 5g, mother chrysanthemum 8g, mountain cornel 12g, date 15g, Radix Glycyrrhizae 15g, the soak by water of 500g is added after cleaning, when decoction liquor evaporation and concentration is to 200g, filter residue, get decoction liquor, after decoction liquor cooling, add boric acid 7g, be uniformly mixed and obtain the specimens preserving liquid of in vitro sample.
Using method:
In vitro pathological tissue sample is directly dipped to 10-15min in the dilution of this product, and the water adding twice when this product uses dilutes, and adjust ph is between 6-8.
Experimental therapy
With the specimens preserving liquid prepared by the present embodiment, process is carried out in vitro sample and preserves.
Experimental program: 10 parts, clinical acquisitions breast cancer tissue sample, is divided into two groups at random, often organizes 5 parts, respectively two kinds of flaking method film-makings, and basis of microscopic observation cellular morphology, situation; Sample preservation is observed for 3 months again simultaneously.
Experimental result: adopt the specimens preserving liquid of the present embodiment to carry out film-making group cellular morphology intact, the original form of cell during sample collection can be kept, do not expand, the metamorphosis such as pyknosis; Still remain intact after 3 months.And control group adopts the display of conventional smear result, cell is overlapping in a large number, and smear also exists a large amount of red blood cells, leucocyte, mucus and the slough etc. that comes off, and pathologist is very difficult when interpretation; After 3 months, sample goes mouldy, cannot film-making observe.
Result judges: adopt the specimens preserving liquid film-making of the present embodiment significantly better than control group, both have significant difference.
More than show and describe general principle of the present invention, principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification is only preference of the present invention; be not used for limiting the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is defined by appending claims and equivalent thereof.
Claims (2)
1. for a specimens preserving liquid for the in vitro sample of medical research, it is characterized in that: be prepared from by the raw material of following mass fraction:
Boric acid 7 parts, galingal 13 parts, honeysuckle 10 parts, selfheal 4 parts, the red sage root 5 parts, mother chrysanthemum 8 parts, mountain cornel 12 parts, 15 parts, date, 15 parts, Radix Glycyrrhizae.
2. the preparation method of the specimens preserving liquid of the in vitro sample for medical research according to claim 1, it is characterized in that: take galingal 13 parts, honeysuckle 10 parts, selfheal 4 parts, the red sage root 5 parts, mother chrysanthemum 8 parts, mountain cornel 12 parts, 15 parts, date, 15 parts, Radix Glycyrrhizae, the soak by water of 500 parts is added after cleaning, during decoction liquor evaporation and concentration to 200 part, filter residue, gets decoction liquor, after decoction liquor cooling, add boric acid 7 parts, be uniformly mixed and obtain the specimens preserving liquid of in vitro sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510224390.5A CN104798768A (en) | 2015-04-29 | 2015-04-29 | Preservation and treatment solution of in-vitro specimen for medical research |
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| CN201510224390.5A CN104798768A (en) | 2015-04-29 | 2015-04-29 | Preservation and treatment solution of in-vitro specimen for medical research |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108353889A (en) * | 2018-03-29 | 2018-08-03 | 蚌埠医学院 | A kind of Traditional-Chinese-medicine-type nano-titanium dioxide specimen preserving liquid |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1415199A (en) * | 2001-10-30 | 2003-05-07 | 上海方富科技有限公司 | A kind of preservative and its prepn. method |
| CN1748500A (en) * | 2005-10-11 | 2006-03-22 | 李甦 | Kidney preserving liquid |
| CN101491238A (en) * | 2009-03-06 | 2009-07-29 | 李立 | Organ preservation liquid |
| CN101496511A (en) * | 2008-01-31 | 2009-08-05 | 任祖翰 | Antisepsis composition for remains as well as preparation method and application |
| CN102067892A (en) * | 2010-12-15 | 2011-05-25 | 华南农业大学 | Anticorrosive Chinese medicine preparation and application thereof |
| CN104521942A (en) * | 2014-12-24 | 2015-04-22 | 博兴县殡仪馆 | Body antiseptic agent |
-
2015
- 2015-04-29 CN CN201510224390.5A patent/CN104798768A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1415199A (en) * | 2001-10-30 | 2003-05-07 | 上海方富科技有限公司 | A kind of preservative and its prepn. method |
| CN1748500A (en) * | 2005-10-11 | 2006-03-22 | 李甦 | Kidney preserving liquid |
| CN101496511A (en) * | 2008-01-31 | 2009-08-05 | 任祖翰 | Antisepsis composition for remains as well as preparation method and application |
| CN101491238A (en) * | 2009-03-06 | 2009-07-29 | 李立 | Organ preservation liquid |
| CN102067892A (en) * | 2010-12-15 | 2011-05-25 | 华南农业大学 | Anticorrosive Chinese medicine preparation and application thereof |
| CN104521942A (en) * | 2014-12-24 | 2015-04-22 | 博兴县殡仪馆 | Body antiseptic agent |
Non-Patent Citations (4)
| Title |
|---|
| 张慧明: "中药抑菌及其在日化产品中的应用", 《第九届中国化妆品学术研讨会论文集》 * |
| 曾荣 等: "植物源防腐剂在果蔬保鲜中应用研究进展", 《中国食品学报》 * |
| 祖丽皮亚.玉努斯: "新疆大沙枣果实抑菌作用研究", 《食品科学》 * |
| 肖中献: "抗病毒中药综述", 《河南医药信息》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108353889A (en) * | 2018-03-29 | 2018-08-03 | 蚌埠医学院 | A kind of Traditional-Chinese-medicine-type nano-titanium dioxide specimen preserving liquid |
| CN108353889B (en) * | 2018-03-29 | 2021-05-25 | 蚌埠医学院 | Traditional Chinese medicine type nano titanium dioxide specimen preserving fluid |
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