CN101869098A - Histopathology sample preservation and treating fluid containing Chinese herbal medicine components - Google Patents
Histopathology sample preservation and treating fluid containing Chinese herbal medicine components Download PDFInfo
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- CN101869098A CN101869098A CN200910050029A CN200910050029A CN101869098A CN 101869098 A CN101869098 A CN 101869098A CN 200910050029 A CN200910050029 A CN 200910050029A CN 200910050029 A CN200910050029 A CN 200910050029A CN 101869098 A CN101869098 A CN 101869098A
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Abstract
The invention relates to a histopathology sample preservation and treating fluid containing Chinese herbal medicine components, which is suitable for the preservation and the treatment of histopathology in vitro samples. The invention has the core content that Chinese herbal medicine components or Chinese herbal medicine extractive components are taken as active components for preserving and treating histopathology samples, various kinds of Chinese herbal medicines can meet the requirements of the invention, and various kinds of compatibility of medicines can achieve the purposes of the invention. The invention is fundamentally different from a preservative technology containing Chinese herbal medicine components. In the invention, the in vitro samples are preserved and treated to keep the samples to be in accordance with living conditions in macroscopic view to the greatest extent, and keep cells to maintain forms and structures before in vitro in microscopic view. Therefore, the target difficulty to be realized by the invention is greater than that of the preservative containing Chinese herbal medicine components.
Description
Technical field
The present invention relates to a kind of histopathology sample preservation and treating fluid that contains medicinal herb components, belong to medical field, be applicable to the processing and the preservation of the stripped sample of Histopathology.
Background technology
Histopathology or claim histopathology is the researching human body pathology subdiscipline that each organ histology of system changes when ill.Its main research means is to carry out observation by light microscope after in vitro tissue is cut into slices after treatment, dyeed, and diagnoses the illness with observed result.Be not difficult to find out thus how handling the in vitro tissue sample well is the prerequisite and the key of diagnosis and research.After the pathology sample exsomatizes and since the variation of microenvironment with autolyze and (or) corruption, make its structural deterioration, simultaneously on microcosmic, cell degradation, self-dissolving.Handle the purpose of sample, in all sorts of ways exactly makes Pathologic specimen keep its preceding state that exsomatizes as far as possible.More crucial is, owing to need microscopic examination, therefore, Histopathology not only requires stripped sample (as color and luster, volume, soft durometer etc.) aspect macroscopical to try one's best near state before exsomatizing, and more requiring will be as far as possible near condition of living organism aspect the microcosmic of cellular level.
At present, formalin is the Histopathology sample process reagent that is most widely used.But because of shortcomings such as its high toxicity, high carcinogenics, damaging user's health always, environment has also been caused very big harm.
Therefore, search out that result of use is good, to preserve reagent treatment be to have very big academic significance and realistic meaning to the Histopathology of low toxicity, low irritant.
In fact, use Chinese herbal medicine, just occur and used as far back as B.C. as the method for preserving tissue or corpse.Mawangdui, the Han dynasty suffering that Pharaoh's remains are preserved, China is unearthed as ancient Egypt chases after the preservation of mummy and the unearthed noble of Qing Dynasty woman corpse preservation or the like, has all used the preservation technology of Chinese herbal medicine or even Chinese herb compound in a large number.Modern document and scientific achievement also have multiple with the application of Chinese herbal medicine as preservative, have obtained good result of use.
Though the present invention has used Chinese herbal medicine too as active ingredient, have basic different with above-mentioned prior art.
Above-mentioned technology and method mainly are as preservative with medicinal herb components.Preservative is to be used for the medicament of inhibiting substances corruption, main effect is to being the inhibition that the microbial growth of metabolism substrate continues with the corrupt substance, its objective is to make the without putrefaction of corpse equal samples on the macroscopic view, never degenerate, keep global indexs such as color and luster, soft durometer simultaneously.And the present invention is used for the sample that exsomatizes is preserved and handled, purpose is not only to keep sample consistent with animation as far as possible on macroscopic view, the more important thing is, to on microcosmic, make form and structure before cell keeps exsomatizing as far as possible, as organelle to preserve, nuclear fine structure (chromosome etc.) is complete, the whole big contraction or expansion or the like that do not take place of cell.Therefore, the target that the present invention will realize and above-mentioned prior art have basic different, and the difficulty of realization is also bigger.
Summary of the invention
Core content of the present invention is that herb ingredients or Chinese herbal medicine extract composition are preserved the active ingredient of handling as the Histopathology sample, has multiple Chinese herbal medicine can satisfy requirement of the present invention, also has multiple compatibility can reach purpose of the present invention.
Through a large amount of experiments and screening, and in conjunction with material elementses such as production cost and production technologies, determined with Chinese herbal medicine common rush, rheum officinale, mustard seed and Poria cocos by a certain percentage the compound extract of compatibility as the active ingredient of this preservation treatment fluid, cooperate aid in treatment composition with strong points simultaneously, reach the preservation processing requirements of Histopathology the sample that exsomatizes.The one-tenth of this histopathology sample preservation and treating fluid be grouped into and compound method as follows:
Main component: the compound extract of common rush (5-10g), rheum officinale (5-10g), mustard seed (4-7g) and Poria cocos (8-15g) (total 1000ml)
Auxiliary element: ethanol (5-15%), methyl alcohol (5-15%), polyethylene glycol (5-10%), lactic acid (5-10%)
Bleeding agent: sylvite or sodium salt (1%)
Humectant: glycerine (3-5%)
Compound method:
1) common rush, rheum officinale, mustard seed and Poria cocos are extracted with distilled water after, concentrated filtrate and decolouring;
2) get part filtrate, after adding auxiliary element, bleeding agent and the humectant, use the distilled water constant volume.
The actual result that uses shows that this histopathology sample preservation and treating fluid can replace formalin to use.It is good that tissue morphology keeps, and dyeing is abundant, and cell structure is clear under the mirror, and structural integrity can satisfy the preservation processing requirements of Histopathology to sample fully.
This product low toxicity, the low stimulation can greatly reduce to the infringement of user's health with to the harm of environment.
Embodiment
Clearly, the present invention will be not limited only to embodiment.
Embodiment following (preparation 1000ml preserves treatment fluid):
1) get common rush 7g, rheum officinale 7g, mustard seed 5g and Poria cocos 10g, add 2000ml distilled water, boil, little fire is endured to 1000ml;
2) repeat water and carry once, merging filtrate, heating is concentrated into 1000ml, and decolouring is stand-by;
3) get extract 100ml, add distilled water 400ml, add sodium chloride 10g, after the stirring and dissolving, add ethanol 100ml, methyl alcohol 100ml, glycerine 50ml, lactic acid 50ml, polyethylene glycol 50ml, stirring and dissolving;
4) be settled to 1000ml with distilled water.
Using method
The pathological tissue sample that exsomatizes directly is dipped in this product gets final product.
Claims (3)
1. a histopathology sample preservation and treating fluid that contains medicinal herb components is characterized in that, contains medicinal herb components or Chinese herbal medicine extract composition.
2. histopathology sample preservation and treating fluid that contains medicinal herb components, it is characterized in that, can not only make sample consistent with animation as far as possible on macroscopic view, more can on microcosmic, make form and structure before cell keeps exsomatizing, as far as possible so that light microscopic is observed down.
3. a histopathology sample preservation and treating fluid that contains medicinal herb components is characterized in that, can preserve the processing composition with other and be mixed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910050029A CN101869098A (en) | 2009-04-27 | 2009-04-27 | Histopathology sample preservation and treating fluid containing Chinese herbal medicine components |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910050029A CN101869098A (en) | 2009-04-27 | 2009-04-27 | Histopathology sample preservation and treating fluid containing Chinese herbal medicine components |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101869098A true CN101869098A (en) | 2010-10-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910050029A Pending CN101869098A (en) | 2009-04-27 | 2009-04-27 | Histopathology sample preservation and treating fluid containing Chinese herbal medicine components |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105519518A (en) * | 2015-04-08 | 2016-04-27 | 上海蓝盎电子科技发展有限公司 | Chinese herbal medicine bacterial fermentation product-containing eco-friendly harmless pathological tissue preservation and treatment solution |
| CN109566597A (en) * | 2018-11-05 | 2019-04-05 | 北京世纪劲得生物技术有限公司 | A kind of endometrial stem cells protection liquid and preparation method thereof |
| CN111771867A (en) * | 2020-07-13 | 2020-10-16 | 许昌学院 | Preservative for biological dissection experiments and preparation method thereof |
-
2009
- 2009-04-27 CN CN200910050029A patent/CN101869098A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105519518A (en) * | 2015-04-08 | 2016-04-27 | 上海蓝盎电子科技发展有限公司 | Chinese herbal medicine bacterial fermentation product-containing eco-friendly harmless pathological tissue preservation and treatment solution |
| CN109566597A (en) * | 2018-11-05 | 2019-04-05 | 北京世纪劲得生物技术有限公司 | A kind of endometrial stem cells protection liquid and preparation method thereof |
| CN111771867A (en) * | 2020-07-13 | 2020-10-16 | 许昌学院 | Preservative for biological dissection experiments and preparation method thereof |
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Application publication date: 20101027 |