CN105519518A - Chinese herbal medicine bacterial fermentation product-containing eco-friendly harmless pathological tissue preservation and treatment solution - Google Patents
Chinese herbal medicine bacterial fermentation product-containing eco-friendly harmless pathological tissue preservation and treatment solution Download PDFInfo
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- CN105519518A CN105519518A CN201510162930.1A CN201510162930A CN105519518A CN 105519518 A CN105519518 A CN 105519518A CN 201510162930 A CN201510162930 A CN 201510162930A CN 105519518 A CN105519518 A CN 105519518A
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Abstract
The invention relates to a Chinese herbal medicine bacterial fermentation product-containing eco-friendly harmless pathological tissue preservation and treatment solution and belongs to the field of medical treatment. The preservation treatment solution is suitable for preservation and treatment on a fresh pathological tissue sample, keeps the original living state of a sample in a macro-level and keeps the original shape and structure of the separated sample cells in a micro-level. The bacterial fermentation product is used as an effective component for pathological tissue sample preservation and treatment and through the modern technology, in short time, industrial production of a high-concentration composite bacterial fermentation product with a function of preserving a fresh tissue sample is realized. A plurality of plant-derived or animal-derived Chinese herbal medicines can be used as raw materials, a plurality of anaerobic bacteria can be selected and other components can be added into the finished product so that different usage demands are satisfied. The preservation and treatment solution develops the traditional Chinese medicine and pharmacy, is eco-friendly, utilizes easily available raw materials, can be used simply and conveniently, has actual use effects the same to those of formalin and has some indexes better than those of formalin.
Description
Technical field
The present invention relates to a kind of environmentally friendly pathological tissue specimens preserving liquid containing Chinese herbal medicine bacterial fermentation thing, belong to medical field, be applicable to process and the preservation of the fresh sample of Histopathology.
Background technology
After fresh sample is in vitro, by autolyze and (or) corruption, make its structural deterioration, simultaneously on microcosmic, cell degradation, self-dissolving.Histopathology or claim histopathology, be researching human body ill time each system organ's histological change a pathology subdiscipline.Its main research means be in vitro tissue (or cell) is carried out after treatment cut into slices (or smear), dye after carry out observation by light microscope, diagnose the illness by observed result.Therefore, Histopathology not only require in vitro sample in macroscopical (as color and luster, volume, soft durometer etc.) as far as possible close to state before in vitro, more requiring will as far as possible close to condition of living organism in the microcosmic of cellular level.Be not difficult to find out thus, the macroscopic view before making Pathologic specimen as far as possible keep it in vitro by various method or microstate are prerequisite and the key of this Subject diagnostic analysis and research.
At present, formalin is the Histopathology sample processing reagent be most widely used.But because of its shortcoming such as high toxicity, high carcinogenic, damage the health of user always, high risks be also result in environment.Meanwhile, through the sample of formalin process, can produce and harden significantly, fade.Therefore, search out that result of use is good, the Histopathology specimens preserving reagent of low toxicity, low irritant has very large academic significance and realistic meaning.
In fact, use natural plants or animality medicinal material as the method for preserving tissue or corpse, just occurred as far back as B.C. and applied.The pungent preservation and the preservation of unearthed Qing Dynasty noble female corpse etc. chasing after mummy in the Mawangdui, Han dynasty that Pharaoh's remains are preserved, China is unearthed as ancient Egypt, employs the Techniques of preserving of Chinese herbal medicine or even Chinese herb compound all in a large number.
In fact, the long-term preservation method of mummy in global range, its principle is not quite similar.As the store method of ancient Egypt, compound herbal medicine or animal drugs just play short time corrosion-resistant effect, really play long-term preservation effect, are dry weathers, finally make corpse become corpse.Therefore, research direction of the present invention is for the pungent store method chasing after mummy in Mawangdui, similar Han dynasty, that is, corpse is finally be immersed in liquid environment to carry out long-term technology of preserving.
Can make the prolonged real composition be stale-proof of corpse in ancient method, the viewpoint of extensively approval at present, thinks all kinds of herbal medicine or animal drugs.But inventor, after long-term research, thinks that above viewpoint is incorrect.Because there is the too many fact to be cannot explain by current viewpoint.Wherein more topmostly be exactly, what put into inner and outer coffin when mummy is fresh is solid-state drug, but after long-term storage, solid-state drug becomes liquid corpse liquid, not original medicine essence completely, in fact original medicine lost efficacy at all, and what therefore really work should be other compositions.
Summary of the invention
Inventor through to corpse liquid and to principal element comprehensive studies such as mummy Conservation environment after, having drawn conclusion diverse with existing viewpoint: in fact play long-term preservation effect, is the composite fermentation thing after anaerobic bacteria is decomposed compound medicine (comprising autonomic drug and animal drugs) fermentation.
Below the forming process that inventor studies the composite fermentation thing drawn:
First, after fresh cadaver puts into inner and outer coffin, around corpse, put into appropriate compound plant medicine or animal drugs.Corpse itself also will carry out inner anticorrosion pretreatment, as poured into traditional Chinese herbal decoction, or fills Chinese herbal medicine etc. in abdomen.Then, sealed by inner and outer coffin plaster etc., or inner and outer coffin inherently having good sealing property, no matter which kind of means, is all reach the object sealed by corpse.Subsequently, coffin chamber is sealed, form sealing anaerobic environment further.Can say that this three measures is the standardization program of noble's funeral in ancient times, through textual research.This exactly can explain, why common people's corpse is difficult to long-term preservation, and trace it to its cause, mainly the height of three step costs like this, implementing difficulty big, is not that general public can be accomplished.But aforementioned three steps, the effect played is short-term corrosion prevention, and compound medicine will soon lose efficacy.Real crucially later change.Next, because anaerobic bacteria is the microorganism extensively existed, especially can have in natural drug, in addition, the impossible bone dry of whole coffin chamber environment, therefore, under anaerobic bacteria, fermentation raw material (compound medicine) and the simultaneous prerequisite of moisture three, as time goes by, fermentation becomes liquid composite fermentation thing gradually.
Show through inventor's experimental study, this fluid-like state composite fermentation thing, its pH value is 3-5, and main component is the multiple types compound amino acid presenting reset condition, and the trace element of abundant species.Corpse is in this fluid-like state composite fermentation thing, and macrostructure's form and micro-cell form all can obtain well protecting and keeping.Compared to the formalin that the modern times use, this fluid-like state composite fermentation thing has the powerful advantages that can make to organize and keep soft and color and luster for a long time, and all the components is eco-friendly natural component, is green and desirable tissue preserration liquid.What is more important, the raw material of this fluid-like state composite fermentation thing is also draw materials natural, and formation condition and production technology are also environmental protection, are the ideal products of pollution-free industry.
Inventor studies discovery, wants to be formed to have the long-term liquid state fermentation thing preserving corpse function, needs to possess following specific condition:
1) environment must anaerobic.
2) raw material can not arbitrarily be chosen, and desirable feedstock should be aromatics or antimicrobial DP finish, and best multi-medicament conbined usage, avoids using single variety as far as possible.
Through historical data verification and the experimental study in inventor's later stage, conclusion is, ancients are generally the medicines by aromatics and anti-microbial type, are never that plant or the animal medicinal material of what kind can be applied.Modern study also shows, aromatics medicine, as cloves, the root of Dahurain angelica, cassia bark etc., all has good antimicrobial preservative efficacy.And pine needle, the achene of Siberian cocklebur, Poria cocos, the root of Chinese wild ginger etc., all there is effect of antiphlogistic antibacterial.
3) desirable bacterial classification is budding fungus, abstains from most to use der Pilz.
The actual experiment of inventor shows, saccharomycetic fermentation product, the most applicable long-term preservation tissue samples.The anaerobic bacteria of other types also can use, but der Pilz is not suitable for.
4) raw material dosage must be suitable, is generally the medicine using 1/4 body weight, otherwise can affect the preservation of corpse because fermentation product is not enough.
Core content of the present invention, is exactly using the active ingredient of Chinese herbal medicine bacterial fermentation thing as the process of Histopathology Sample preservation, and applies modern crafts, industrially produces the high concentration composite fermentation thing having and preserve flesh tissue sample function in short-term.
Raw material of the present invention comprises:
1) anaerobic bacterial classification.Most preferably be saccharomycete.
2) aromatics and anti-microbial type autonomic drug or animal drugs.As mustard seed, rheum officinale, rush, Poria cocos, cloves, Chinese cassia tree etc.
Main production of the present invention:
Weight ratio is the Chinese herbal medicine of 10%-35%, shreds the coarse fodder into 1-2cm, puts into fermentation tank.In fermentation tank, add the bacterial classification that weight ratio is 0.3%-1%, then add excess water.Fermentation tank is sealed, after 3 months, liquid composite fermentation thing can be produced.Extract zymotic fluid out, after filtration, be finished product.
In technique of the present invention, the weight ratio of each component does not need accurate especially, but most preferably is: Chinese herbal medicine 30%, bacterial classification 0.5%, water surplus.Wherein, water quality has no special requirements, clean, as running water.
Finished product of the present invention, also can be mixed with other chemicals, to develop the product of difference in functionality or hold capacity, can osmotic pressure be improved as added sodium chloride, add the wettability that glycerine, propane diols etc. can increase tissue samples, adding ethanol, methyl alcohol, lactic acid etc. can strengthen retention etc.
Embodiment
Embodiment 1 is to produce the liquid composite fermentation thing of 100kg.
Get Chinese herbal medicine mustard seed, rheum officinale, rush, Poria cocos, cloves, Chinese cassia tree, the root of Dahurain angelica, each 7kg, be ground into below 2cm meal, add in fermentation tank.Add 0.8kg barms and 120kg running water again.Sealing and fermenting tank, places 3-4 month under normal temperature.Extract composite fermentation liquid out, filter, filling.Finished product is brown or brown liquid, has Chinese herbal medicine smell, and pH value is 3-5.
Embodiment 2, by the finished product in embodiment 1, in every 100kg, adds 1kg sodium chloride, 1L glycerine, 1L lactic acid, 10kg ethanol, 10kg methyl alcohol, mixing.
Raw material selection of the present invention and bacterial classification selection and comparison extensively, are not limited to embodiment.
Using method
In vitro pathological tissue sample is directly dipped in this product.As long as finished product of the present invention is under air-proof condition, its term of validity at least 10 years.
Result of use
Product of the present invention was through the long-term preservation demonstration test of 3 years, and not only macroscopical preservation effect is better than formalin, and contrast under the microscope after HE section, its preservation effect is also identical with formalin.Result shows, finished product of the present invention can substitute formalin completely and use, and is a kind of excellent and tissue preserration treatment fluid of environmental protection.
Accompanying drawing explanation
The tissue samples preservation effect of finished product of the present invention and formalin contrasts photo.
To be postmortem sample liver and kidney soak the preservation effect after 2 years through finished product of the present invention to Fig. 1.Complete human body's liver samples (left side) and kidney sample (right side) are after finished product of the present invention soaks 2 years, and outward appearance is without significant change.After HE section, the dyeing capacity in haematoxylin and Yihong is strong, and bright-colored, microcosmic preservation effect is good.
Fig. 2 is finished product of the present invention and formalin-fixed Contrast on effect.After conventional embedding is dyeed with HE, the sample that the present invention soaks, eosin stains is obviously more bright-coloured than dipped into formalin.Left side two width figure be human liver (on) and human body lungs (under) after finished product of the present invention soaks 48 hours, HE dicing effect.Right side two width figure be human liver (on) and human body lungs (under) through dipped into formalin after 48 hours, HE dicing effect.
Claims (5)
1., containing an environmentally friendly pathological tissue specimens preserving liquid for Chinese herbal medicine bacterial fermentation thing, it is characterized in that, containing bacterial fermentation thing.
2., containing an environmentally friendly pathological tissue specimens preserving liquid for Chinese herbal medicine bacterial fermentation thing, it is characterized in that, the raw material being used for carrying out fermenting is plant or animal, comprises Chinese herbal medicine.
3., containing an environmentally friendly pathological tissue specimens preserving liquid for Chinese herbal medicine bacterial fermentation thing, it is characterized in that, the bacterium being used for carrying out fermenting is the anaerobic bacteria with Fermentation Function.
4. the environmentally friendly pathological tissue specimens preserving liquid containing Chinese herbal medicine bacterial fermentation thing, it is characterized in that, sample can not only be made macroscopically as far as possible consistent with animation, cell more can be made on microcosmic as far as possible to keep the Morphology and structure in vitro, so that light Microscopic observation.
5., containing the environmentally friendly pathological tissue specimens preserving liquid of Chinese herbal medicine bacterial fermentation thing, it is characterized in that, can with other specimens preserving composition mixtures.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001030996A2 (en) * | 1999-10-26 | 2001-05-03 | Bulla Lee A Jr | Microbial exopolysaccharide and uses thereof |
| CN101869098A (en) * | 2009-04-27 | 2010-10-27 | 上海蓝盎电子科技发展有限公司 | Histopathology sample preservation and treating fluid containing Chinese herbal medicine components |
| CN102908549A (en) * | 2012-11-14 | 2013-02-06 | 蒋常德 | Chinese herbal medicine active fermentation health care liquid for preventing and treating bird flu and preparation method of same |
| CN104351472A (en) * | 2014-10-13 | 2015-02-18 | 湖南省微生物研究院 | Bacillus subtilis fermentation broth of Chinese herbal medicine as well as preparation and application of bacillus subtilis fermentation broth |
-
2015
- 2015-04-08 CN CN201510162930.1A patent/CN105519518A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001030996A2 (en) * | 1999-10-26 | 2001-05-03 | Bulla Lee A Jr | Microbial exopolysaccharide and uses thereof |
| CN101869098A (en) * | 2009-04-27 | 2010-10-27 | 上海蓝盎电子科技发展有限公司 | Histopathology sample preservation and treating fluid containing Chinese herbal medicine components |
| CN102908549A (en) * | 2012-11-14 | 2013-02-06 | 蒋常德 | Chinese herbal medicine active fermentation health care liquid for preventing and treating bird flu and preparation method of same |
| CN104351472A (en) * | 2014-10-13 | 2015-02-18 | 湖南省微生物研究院 | Bacillus subtilis fermentation broth of Chinese herbal medicine as well as preparation and application of bacillus subtilis fermentation broth |
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