CN105795306A - Natural plant-derived antiseptic and its preparation method and use - Google Patents
Natural plant-derived antiseptic and its preparation method and use Download PDFInfo
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Abstract
The invention relates to a natural plant-derived antiseptic. The natural plant-derived antiseptic comprises, by weight, 40-50 parts of begonia fimbristipula extract, 20-30 parts of orange peel extract and 60-80 parts of distilled water. The invention also relates to a preparation method of the natural plant-derived antiseptic and a use of the natural plant-derived antiseptic in aquatic product corrosion resistance. Effective components of Begoniafimbristioula Hance leaves and orange peel are extracted by an alcohol extraction method and through appropriate compatibility, the natural plant-derived antiseptic has good bacteriostasis and antibacterial effects. The natural plant-derived antiseptic can effectively inhibit common bacteria in collichthys lucidus, effectively prolongs a storage life and keeps the original color and local flavor. The natural plant-derived antiseptic utilizes pure natural Begoniafimbristioula Hance and orange peel as raw materials with wide sources, has a low cost, can be prepared through simple processes and can be massively used for food corrosion resistance and especially for aquatic product corrosion resistance.
Description
Technical field
The present invention relates to a kind of natural antiseptic agent, particularly relate to a kind of natural plant source antiseptic agent and its preparation method and application.
Background technology
One of food antiseptic main task being always up food industry, owing to food apoilage is mainly caused by microorganism, therefore use cost is low, application is convenient, respond well chemical preservative becomes food industry and prevents the Main Means of food apoilage.But, chemical preservative drawback in food safety manifests gradually, with a few class preservative (benzoic acid, sorbic acid and its esters main in current food industry, p-Hydroxybenzoate), different hidden danger is there is in these a few class preservative in food safety, such as benzoic acid and its esters, data is had to show: low dosage, long-time sodium benzoate of taking in can affect hereditary material form and the conformation of rat sexual cell, bring out Offspring rats form partition.Therefore, the preservative that exploitation good antimicrobial effect, safety is high becomes the main development direction of food preservative from now on.
For source, food preservative can be divided three classes: chemical preservative, natural antiseptic agent of plant source and the animal sources natural antiseptic agent namely widely applied at present, after wherein, two classes belong to the study hotspot in current food preservative field.For application prospect and application present situation, due to animal sources natural antiseptic agent mainly based on various protein peptide, and this type of biological preservative extraction cost is high, farther out, thus natural antiseptic agent of plant source is the natural antiseptic agent being hopeful to be applied to food industry in significant period of time from now in distance practical application.This is owing to first China's plant resources is extremely abundant, and the long-term practice of working people has been found that the plant that large number of natural plants is especially representative with Chinese tradition Chinese herbal medicine all has obvious antiseptic and inhibiting bacteria function effect；Secondly, comparing with animal sources natural antiseptic agent, the extraction cost of natural antiseptic agent of plant source substantially reduces, and establishes economic base for large-scale practical application；Finally, natural antiseptic agent of plant source fungistatic effect is better, all has obvious advantage in antimicrobial spectrum, effective Mlc etc..Therefore, with natural plants for raw material, extracting exploitation natural antiseptic agent becomes the mainstream development direction of food preservative from now on.
Radix Semiaquilegiae (BegoniafimbristioulaHance) is Begoniaceae Begonia plant, sweet in the mouth, micro-acid, cool in nature, have effect of heat-clearing and toxic substances removing, nourishing the lung to arrest cough, dissipating blood stasis for subsidence of swelling, promoting the production of body fluid to quench thirst, exogenous high fever, heatstroke fever can be controlled, cough due to lung-heat, cold hoarseness, carbuncle sore tumefacting virus, the disease such as treating swelling and pain by traumatic injury.Simultaneously, Radix Semiaquilegiae mineral matter nutritional relatively horn of plenty, the nutrient composition contents such as the calcium of trace element, manganese, ferrum, zinc, copper are higher, especially contain abundant Flavonoid substances, have the effect improving body immunity, heat-clearing and toxic substances removing, promoting blood circulation and hemostasis, blood pressure lowering, blood fat reducing etc..The patent No. is that Gynura bicolor extract can as removing the additive of free radical in cigarette smoke disclosed in the Chinese invention patent " a kind of Gynura bicolor extract and remove the purposes of free radical in cigarette smoke " of ZL201010169076.9 (authorize public number for CN101828764B).The current application about Gynura bicolor extract as food preservative, especially the application in marine product anticorrosion has no report, therefore Radix Semiaquilegiae is studied further, and exploitation novel plant source natural antiseptic agent is significant.
Summary of the invention
The technical problem to be solved is to provide a kind of antibacterial bacteriostatic effective for prior art, and can effectively keep food original flavor nutrition composition, nontoxic natural plant source antiseptic agent.
The preparation method that another technical problem to be solved by this invention is to provide a kind of above-mentioned natural plant source antiseptic agent.
Yet another technique problem to be solved by this invention is to provide the application of a kind of above-mentioned natural plant source antiseptic agent.
This invention address that the technical scheme that above-mentioned first technical problem adopts is: a kind of natural plant source antiseptic agent, it is characterized in that being made up of the raw material of following parts by weight: Gynura bicolor extract 40～50 parts, Pericarpium Citri tangerinae extract 20～30 parts, distilled water 60～80 parts.
As preferably, described Radix Semiaquilegiae chooses Radix Semiaquilegiae leaf, and Radix Semiaquilegiae leaf contains trace element and the flavonoid glycosides such as abundant vitamin, potassium, calcium, ferrum, phosphorus, and therefore the present invention selects Radix Semiaquilegiae leaf to be raw material in Gynura bicolor extract extraction process.
This invention address that the technical scheme that second technical problem adopts is: the preparation of (1) Gynura bicolor extract: take fresh Radix Semiaquilegiae leaf and clean, drain, in 70～80 DEG C of drying and processing 24～48h, pulverize after cooling, cross 40～60 mesh sieves, obtain Radix Semiaquilegiae powder；Take appropriate Radix Semiaquilegiae powder, add 70～80% ethanol by solid-liquid ratio 1:20～30, in 60～70 DEG C of reflux, extract, return time is 1.5～2h, sucking filtration, and residue repeats to extract 2 times, filtrate merges, and concentrates in 60～70 DEG C of rotary evaporations, and lyophilization obtains Gynura bicolor extract；(2) preparation of Pericarpium Citri tangerinae extract: take fresh peel of Citrus reticulata Blanco, in 70～80 DEG C of drying and processing 24～48h, pulverizes after cooling, crosses 40～60 sieves, obtains peel of Citrus reticulata Blanco powder；Take appropriate peel of Citrus reticulata Blanco powder, add 70%～90% ethanol by solid-liquid ratio 1:5～10, in 60～70 DEG C of reflux, extract, return time is 1～1.5h, sucking filtration, and residue repeats to extract 2 times, filtrate merges, and concentrates in 60～70 DEG C of rotary evaporations, and lyophilization obtains Pericarpium Citri tangerinae extract；(3) Gynura bicolor extract prepared in step (1), (2) and Pericarpium Citri tangerinae extract carry out group by above-mentioned mass fraction proportioning and join, and put into appropriate distilled water and are stirred dissolving and obtain described natural plant source antiseptic agent.
This invention address that the 3rd technical scheme that technical problem adopts is: the application in aquatic products anticorrosion of this natural plant source antiseptic agent.
Compared with prior art, it is an advantage of the current invention that: the present invention extracts the effective ingredient in Radix Semiaquilegiae leaf and Pericarpium Citri tangerinae by alcohol extracting method, especially Radix Semiaquilegiae rich in the natural product composition such as flavonoid glycoside and alkaloid, and containing limonene in Pericarpium Citri tangerinae, hesperidin, chromocor compound and vitamin C, vitamin B1 etc., and Gynura bicolor extract can effectively extend ascorbic effect, therefore both, compatibility can play good bacteriostasis antibiosis effect in proper proportions, the common bacteria in prunus mume (sieb.) sieb.et zucc. fish can be effectively suppressed by the natural plant source antiseptic agent in the known present invention of embodiment, effectively extend its storage life, and keep original color and luster and local flavor.Additionally, this preservative adopts the Radix Semiaquilegiae of pure natural and Pericarpium Citri tangerinae to be raw material, raw material sources are wide, and cost is low, and preparation process is simple, can be applied in food antiseptic on a large scale, especially the anticorrosion of aquatic products.
Accompanying drawing explanation
Fig. 1 is the impact that natural plants based bacteriostat processes after prunus mume (sieb.) sieb.et zucc. fish is oppressed on enterobacteria；
Fig. 2 is the impact that natural plants based bacteriostat processes after prunus mume (sieb.) sieb.et zucc. fish is oppressed on pseudomonas；
Fig. 3 is the impact that natural plants based bacteriostat processes after prunus mume (sieb.) sieb.et zucc. fish is oppressed on total plate count；
Fig. 4 is the natural plant source antiseptic agent impact (rip cutting, × 50) on prunus mume (sieb.) sieb.et zucc. fish tissue organizational structure；
Fig. 5 is the prunus mume (sieb.) sieb.et zucc. fish tissue organization chart (rip cutting, × 50) of matched group.
Detailed description of the invention
Below in conjunction with accompanying drawing embodiment, the present invention is described in further detail.
Embodiment 1:
Taking fresh Radix Semiaquilegiae leaf to clean, drain, in thermostatic drying chamber, temperature is 70 DEG C, and drying time is 36 hours, pulverizes after cooling, crosses 40 mesh sieves, obtains Radix Semiaquilegiae powder；Taking appropriate Radix Semiaquilegiae powder, be that 1:30 adds 70% ethanol by solid-liquid ratio, in 70 DEG C of reflux, extract, return time is 1.5 hours, sucking filtration, and residue repeats to extract 2 times.Filtrate merges, and concentrates in 60 DEG C of rotary evaporations, and lyophilization obtains Gynura bicolor extract.
Take fresh peel of Citrus reticulata Blanco, in 70 DEG C of drying and processing 36h, pulverize after cooling, cross 40 sieves, obtain peel of Citrus reticulata Blanco powder；Taking appropriate peel of Citrus reticulata Blanco powder, add 80% ethanol by solid-liquid ratio 1:5, in 70 DEG C of reflux, extract, return time is 1h, sucking filtration, and residue repeats to extract 2 times, and filtrate merges, and concentrates in 60 DEG C of rotary evaporations, and lyophilization obtains Pericarpium Citri tangerinae extract.
Take 40 parts of Gynura bicolor extracts, 20 parts of Pericarpium Citri tangerinae extracts, add 60 parts of distilled water, at 60 DEG C, fully dissolve to obtain natural plant source antiseptic agent.
Embodiment 2:
Taking fresh Radix Semiaquilegiae leaf to clean, drain, in thermostatic drying chamber, temperature is 80 DEG C, and drying time is 48 hours, pulverizes after cooling, crosses 60 mesh sieves, obtains Radix Semiaquilegiae powder；Taking appropriate Radix Semiaquilegiae powder, be that 1:20 adds 80% ethanol by solid-liquid ratio, in 60 DEG C of reflux, extract, return time is 2 hours, sucking filtration, and residue repeats to extract 2 times.Filtrate merges, and concentrates in 70 DEG C of rotary evaporations, and lyophilization obtains Gynura bicolor extract.
Take fresh peel of Citrus reticulata Blanco, in 80 DEG C of drying and processing 24h, pulverize after cooling, cross 60 sieves, obtain peel of Citrus reticulata Blanco powder；Taking appropriate peel of Citrus reticulata Blanco powder, add 90% ethanol by solid-liquid ratio 1:7, in 70 DEG C of reflux, extract, return time is 1.5h, sucking filtration, and residue repeats to extract 2 times, and filtrate merges, and concentrates in 70 DEG C of rotary evaporations, and lyophilization obtains Pericarpium Citri tangerinae extract.
Take 45 parts of Gynura bicolor extracts, 30 parts of Pericarpium Citri tangerinae extracts, add 70 parts of distilled water, at 60 DEG C, fully dissolve to obtain natural plant source antiseptic agent.
Embodiment 3:
Taking fresh Radix Semiaquilegiae leaf to clean, drain, in thermostatic drying chamber, temperature is 75 DEG C, and drying time is 24 hours, pulverizes after cooling, crosses 40 mesh sieves, obtains Radix Semiaquilegiae powder；Taking appropriate Radix Semiaquilegiae powder, be that 1:25 adds 75% ethanol by solid-liquid ratio, in 65 DEG C of reflux, extract, return time is 1.7 hours, sucking filtration, and residue repeats to extract 2 times.Filtrate merges, and concentrates in 65 DEG C of rotary evaporations, and lyophilization obtains Gynura bicolor extract.
Take fresh peel of Citrus reticulata Blanco, in 75 DEG C of drying and processing 48h, pulverize after cooling, cross 40 sieves, obtain peel of Citrus reticulata Blanco powder；Taking appropriate peel of Citrus reticulata Blanco powder, add 80% ethanol by solid-liquid ratio 1:10, in 65 DEG C of reflux, extract, return time is 1.3h, sucking filtration, and residue repeats to extract 2 times, and filtrate merges, and concentrates in 65 DEG C of rotary evaporations, and lyophilization obtains Pericarpium Citri tangerinae extract.
Take 50 parts of Gynura bicolor extracts, 25 parts of Pericarpium Citri tangerinae extracts, add 80 parts of distilled water, at 60 DEG C, fully dissolve to obtain natural plant source antiseptic agent.
Embodiment 4:
Under gnotobasis, weighing 5g and mince prunus mume (sieb.) sieb.et zucc. fish and oppress in beaker, appoint the natural plant source antiseptic agent of preparation in Example 1,2,3 to add in beaker, in prunus mume (sieb.) sieb.et zucc. fish flesh of fish preservative, 20～30min is placed in submergence.The prunus mume (sieb.) sieb.et zucc. fish flesh of fish after being soaked by preservative is pulled out, is drained, put in another beaker, then measure 45mL sterile saline to inject, and with after refiner homogenizing, diluent 1mL is drawn with liquid-transfering gun, slowly being injected in the sterile test tube containing 9mL sterile saline, slight jolting mixes, and makes the even liquid of sample of 1:100.Repeat aforesaid operations step, make the dilute solution being incremented by step by step with 10 times of gradients.Then 2～3 acceptable diluent degree solution are chosen, the even liquid of 1mL sample is drawn in sterilizing plate with liquid-transfering gun, pour into different selective mediums, rotating plate in time makes it shake up, stand cooling after its solidification, plate it is inverted and puts in constant incubator, cultivating a period of time under preference temperature, carry out colony counting.
This experiment is respectively adopted nutrient agar to calculate total number of bacteria, VRBGA culture medium cultivates enterobacteria, Pseudomonades agar culture medium cultivates pseudomonas, condition of culture is: 30 DEG C and 48h, arranging the prunus mume (sieb.) sieb.et zucc. fish flesh of fish sample soaked without preservative simultaneously and (add the sterile purified water with preservative equivalent) as a control group, operating process is ibid.
nullExperimental result is Fig. 1 such as、Shown in Fig. 2 and Fig. 3，Wherein Fig. 1 is the impact that antibacterial processes after prunus mume (sieb.) sieb.et zucc. fish is oppressed on enterobacteria，Fig. 2 is the impact that antibacterial processes after prunus mume (sieb.) sieb.et zucc. fish is oppressed on pseudomonas，Fig. 3 is the impact that antibacterial processes after prunus mume (sieb.) sieb.et zucc. fish is oppressed on total plate count，By Fig. 1、Fig. 2 and Fig. 3 can obtain，Storage 3、6、9、12、15、When 18 days，The enterobacteria of the prunus mume (sieb.) sieb.et zucc. fish soaked、Pseudomonas、Total plate count is respectively less than unsoaked prunus mume (sieb.) sieb.et zucc. fish，Not soaking the prunus mume (sieb.) sieb.et zucc. fish enterobacteria when storing 18 days is 5.12lgCFU/g、Pseudomonas is 4.58lgCFU/g、Total plate count is 6.814lgCFU/g，Soaking the prunus mume (sieb.) sieb.et zucc. fish of the natural plant source antiseptic agent enterobacteria when preserving 18 days is 3.56lgCFU/g、Pseudomonas is 3.04lgCFU/g、Total plate count is 6.205lgCFU/g，Illustrate that antibacterial is had obvious inhibitory action by the natural plant source antiseptic agent in the present invention.
Take respectively and preserve two groups of prunus mume (sieb.) sieb.et zucc. fish samples to the 8th day, at same position, the prunus mume (sieb.) sieb.et zucc. fish flesh of fish is cut into the cuboid of 0.3cm × 0.2cm × 0.2cm, at 4 DEG C, 24h is fixed with 0.2mol/L phosphate buffer (containing 2.5% glutaraldehyde, pH7.4) in 1.5mL centrifuge tube.Then follow these steps to process:
(1) rinsing: outwell fixative, rinses 3 times with 0.1mol/LPBS buffer, each 15min；
(2) serial dehydration: with the ethanol of 30%, 50%, 70%, 80%, 90% dehydration step by step after rinsing, each 10～15min, then with dehydrated alcohol dehydration 2 times, each 10min；
(3) displacement: successively with the dehydrated alcohol of different proportion (3:1,1:1,1:3) and tert-butyl alcohol mixed solution, replacing dehydrated alcohol, each 10min step by step, then replace 1 time with the pure tert-butyl alcohol, the time is 10min；
(4) cover sample with a small amount of tert-butyl alcohol, put into refrigerator freezing, place into the dry 48h of freezer dryer.Then viscous platform, uses ion sputtering instrument metal spraying, is finally scanned ultramicroscope (SEM) and observes, take pictures.
As shown in Figure 4 and Figure 5, from fig. 4, it can be seen that the muscular tissue structure soaking the prunus mume (sieb.) sieb.et zucc. fish of natural plant source antiseptic agent is complete, muscle fiber arrangement is careful closely, and Fig. 5 shows for experimental result, and the arrangement of unsoaked prunus mume (sieb.) sieb.et zucc. fish (matched group) muscle fiber becomes loose.The muscular histological characteristics of the flesh of fish is the important indicator evaluating its quality, and it is the basis of flesh of fish Meat change, is also the indirect reflection of muscle protein degraded situation.Test result indicate that, can effectively suppress the deterioration of myofibrillar degraded and organizational structure in the prunus mume (sieb.) sieb.et zucc. fish flesh of fish with the preservative that Radix Semiaquilegiae and Pericarpium Citri tangerinae are raw material, better keep flesh quality.
Claims (4)
1. a natural plant source antiseptic agent, it is characterised in that be made up of the raw material of following parts by weight: Gynura bicolor extract 40～50 parts, Pericarpium Citri tangerinae extract 20～30 parts, distilled water 60～80 parts.
2. natural plant source antiseptic agent as claimed in claim 1, it is characterised in that: described Radix Semiaquilegiae chooses fresh Radix Semiaquilegiae leaf.
3. the preparation method of a natural plant source antiseptic agent as claimed in claim 1, it is characterised in that comprise the following steps:
(1) preparation of Gynura bicolor extract: take fresh Radix Semiaquilegiae leaf and clean, drain, in 70～80 DEG C of drying and processing 24～48h, pulverizes after cooling, crosses 40～60 mesh sieves, obtain Radix Semiaquilegiae powder；Take appropriate Radix Semiaquilegiae powder, add 70～80% ethanol by solid-liquid ratio 1:20～30, in 60～70 DEG C of reflux, extract, return time is 1.5～2h, sucking filtration, and residue repeats to extract 2 times, filtrate merges, and concentrates in 60～70 DEG C of rotary evaporations, and lyophilization obtains Gynura bicolor extract；
(2) preparation of Pericarpium Citri tangerinae extract: take fresh peel of Citrus reticulata Blanco, in 70～80 DEG C of drying and processing 24～48h, pulverizes after cooling, crosses 40～60 sieves, obtains peel of Citrus reticulata Blanco powder；Take appropriate peel of Citrus reticulata Blanco powder, add 70%～90% ethanol by solid-liquid ratio 1:5～10, in 60～70 DEG C of reflux, extract, return time is 1～1.5h, sucking filtration, and residue repeats to extract 2 times, filtrate merges, and concentrates in 60～70 DEG C of rotary evaporations, and lyophilization obtains Pericarpium Citri tangerinae extract；
(3) Gynura bicolor extract prepared in step (1), (2) and Pericarpium Citri tangerinae extract carry out group by the mass fraction proportioning in claim 1 and join, and put into appropriate distilled water and are stirred dissolving to obtain described natural plant source antiseptic agent.
4. a natural plant source antiseptic agent as claimed in claim 1 application in aquatic products anticorrosion.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107439897A (en) * | 2017-07-27 | 2017-12-08 | 磐安县派普特生物科技有限公司 | A kind of plant-based bacteriostat |
| CN108094525A (en) * | 2017-12-25 | 2018-06-01 | 西北农林科技大学 | A kind of plant source mycotoxin inhibitors and its application |
| CN110604239A (en) * | 2019-10-31 | 2019-12-24 | 宁夏回春生物科技有限公司 | Preservative-free perfume pear juice beverage and preparation method thereof |
| CN111296408A (en) * | 2020-02-17 | 2020-06-19 | 广东海洋大学深圳研究院 | Specimen environment-friendly composite mildew-proof preservative of natural plant extract and application thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110604239A (en) * | 2019-10-31 | 2019-12-24 | 宁夏回春生物科技有限公司 | Preservative-free perfume pear juice beverage and preparation method thereof |
| CN111296408A (en) * | 2020-02-17 | 2020-06-19 | 广东海洋大学深圳研究院 | Specimen environment-friendly composite mildew-proof preservative of natural plant extract and application thereof |
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