CN110237121A - A kind of anti-mite anti acne of traditional Chinese medicine composition and the preparation method and application thereof - Google Patents
A kind of anti-mite anti acne of traditional Chinese medicine composition and the preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a kind of anti-mite anti acne of traditional Chinese medicine compositions and the preparation method and application thereof, belong to daily chemicals field.The Chinese medicine composition includes by 5~35 parts of rosemary of traditional Chinese medicinal components Cheng ﹕ of following parts by weight, 5~35 parts of dandelion, 5~35 parts of Radix Isatidis, 5~35 parts of glaucousleaf hydrangeavine stem, 5~25 parts and 5~25 parts of Radix Glycyrrhizae of paris polyphylla.The present invention is that guidance carries out prescription with Chinese medicine monarch theory, is acted synergistically by plurality of Chinese, achievees the purpose that anti-mite anti-acne.Present invention employs microjet extraction methods to prepare extracting solution, and is concentrated with film concentration-macroporous resin adsorption-film, obtains Chinese medicine composition.Chinese medicine composition of the invention can effectively remove face's acarid, inhibit propionibacterium acnes, and have the effects that clearing heat and detoxicating, anti-inflammatory, and non-stimulated to skin safe, for having good anti-mite acne-removing in daily necessities.
Description
Technical field
The invention belongs to a kind of Chinese medicine composition, a kind of anti-mite anti acne of traditional Chinese medicine composition and its preparation side are particularly related to
Method and application, belong to daily chemicals field.
Background technique
Acne is being commonly called as acne, is called " whelk ", " dark sore " or " acne ", is due to hair follicle and sebum adenemphraxis, hair
A kind of skin disease that inflammation is caused.When puberty, superfluous hormone can stimulate hair fast-growth in vivo, and smegma is more
Therefore many greases of sauce, hair and sebaceous glands accumulate excessive substance, grease and bacterium is made to be easier to adhere to, and cause the reaction of red swelling of the skin.
Since this symptom is common in youth of both sexes, so just it is referred to as " whelk ".Anti-acne is eliminated with medical technology or method
The process of whelk.
Human skin acarid and the human body face problem: such as epifolliculitis, seborrhea, alopecia, blear-eye, external ear
Road itch etc. is especially related very closely with acne, brandy nose.Acarid multiparasitization is drawn in pilosebaceous follicle
Intracellular nutritional substance and smegma object, and damaged normal cell；And mechanical stimulus and polypide excreta due to polypide
Chemical irritation effect, parasitic site can cause hair follicle expand, blood vessel dilatation peripheral cell infiltration, proliferation of fibrous tissue, and
Make tissue damage, cause allergic reaction, phenomena such as red grouper, papule, granuloma, warts and itch occurs in skin.
Summary of the invention
The primary purpose of the present invention is that provide a kind of anti-mite anti acne of traditional Chinese medicine composition, with significant anti-mite, it is antibacterial,
Anti-inflammatory, heat-clearing toxin-expelling functions, and safety with higher.
In order to achieve the above object of the invention, this invention takes following Ji art Fang An ﹕
A kind of anti-mite anti acne of traditional Chinese medicine composition, including by 5~35 parts of rosemary of traditional Chinese medicinal components Cheng ﹕ of following parts by weight,
5~35 parts of dandelion, 5~35 parts of Radix Isatidis, 5~35 parts of glaucousleaf hydrangeavine stem, 5~25 parts and 5~25 parts of Radix Glycyrrhizae of paris polyphylla.
Preferably, a kind of anti-mite anti acne of traditional Chinese medicine composition, including being changed by the traditional Chinese medicinal components Cheng ﹕ fan of following parts by weight
Fragrant 10~30 parts, 10~30 parts of dandelion, 10~30 parts of Radix Isatidis, 10~30 parts of glaucousleaf hydrangeavine stem, 10~25 parts of paris polyphylla and
10~25 parts of Radix Glycyrrhizae.
Preferably, a kind of anti-mite anti acne of traditional Chinese medicine composition, including being changed by the traditional Chinese medicinal components Cheng ﹕ fan of following parts by weight
It is 20 parts fragrant, 20 parts of dandelion, 15 parts of Radix Isatidis, 15 parts of glaucousleaf hydrangeavine stem, 15 parts and 15 parts of Radix Glycyrrhizae of paris polyphylla.
Preferably, a kind of anti-mite anti acne of traditional Chinese medicine composition, including being changed by the traditional Chinese medicinal components Cheng ﹕ fan of following parts by weight
It is 25 parts fragrant, 20 parts of dandelion, 18 parts of Radix Isatidis, 12 parts of glaucousleaf hydrangeavine stem, 15 parts and 10 parts of Radix Glycyrrhizae of paris polyphylla.
Preferably, a kind of anti-mite anti acne of traditional Chinese medicine composition, including being changed by the traditional Chinese medicinal components Cheng ﹕ fan of following parts by weight
It is 18 parts fragrant, 22 parts of dandelion, 15 parts of Radix Isatidis, 15 parts of glaucousleaf hydrangeavine stem, 18 parts and 12 parts of Radix Glycyrrhizae of paris polyphylla.
Second object of the present invention is to be to provide a kind of preparation method of anti-mite anti acne of traditional Chinese medicine composition.
In order to reach this purpose of the invention, this invention takes following technical solutions:
Using microjet extraction method, i.e., after Chinese medicine compound prescription mixture being carried out swollen quick-fried extraction, centrifugal filtration, then use
The concentration of film concentration-macroporous resin adsorption-film, obtains Chinese medicine composition；
Specifically comprise the following steps:
1. ingredient: by the removal of impurities of each Chinese medicine material, cleaning, drying, smashing it through 40~100 meshes, mix, it is multiple to obtain Chinese medicine
Square mixture；
2. extracting: Chinese medicine compound prescription mixture and solvent being mixed and impregnated, then by the mixed of Chinese medicine compound prescription mixture and solvent
Liquid is closed by continous way room temperature microjet extraction device, precipitating is abandoned in centrifugal filtration, collects filtrate；
3. purification: filtrate is taken, is concentrated with film, concentrate macroporous resin adsorption, then the second for being 10-95% with volume fraction
Alcohol repeatedly elutes, collect eluent, will elution liquid film be concentrated into Chinese medicine identical weight, add and concentrate identical weight
Butanediol dissolution, is filtered to get anti-mite anti acne of traditional Chinese medicine composition.
Preferably, step 2. described in Chinese medicine compound prescription mixture and solvent press the weight proportion of 1:1~15, soaking time 1
~5 hours, the solvent was one of water, ethyl alcohol, water-ethanol admixture, 500~3000 turns/min of centrifugal rotational speed；
Step 3. described in filtrate with film be concentrated into the 1/5 of original volume；
The step 1.~3. carry out at room temperature.
Third object of the present invention is that this kind of anti-mite anti acne of traditional Chinese medicine composition is applied in daily necessities, provides a kind of tool
There are the daily necessities of anti-mite acne-removing.
In order to reach this purpose of the invention, this invention takes following technical solutions:
A kind of anti-mite anti acne of traditional Chinese medicine composition is preparing the application in daily necessities, and the anti-mite anti acne of traditional Chinese medicine composition is in day
Additive amount in articles is 0.1~25% based on mass fraction.
The daily necessities are as follows: handmade soap, aqua, type of latex type, cream kind, gel class, powder class, powder compacting class, facial mask,
Spray-like, washes shield class at essence class.
Preferably, the present invention provides a kind of anti-mite anti-acne essence, the component including following mass fraction meter:
Preferably, the present invention provides a kind of anti-mite anti-acne handmade soap, the component including following mass fraction meter:
Mechanism of the invention is:
Chinese medicine composition of the invention is to carry out prescription using Chinese medicine monarch theory, and wherein rosemary is monarch drug in a prescription, tool
There is good anti-mite insect-expelling function；Dandelion and Radix Isatidis are ministerial drug, and dandelion, which has effects that significantly to sterilize, to be inhibited, Radix Isatidis
With good anti-inflammatory, antibacterial and itching-relieving efficacies；Glaucousleaf hydrangeavine stem and paris polyphylla are adjutant, and glaucousleaf hydrangeavine stem has heat-clearing and damp-drying drug, rushes down
The effect of fire removing toxic substances, paris polyphylla has effects that extraordinary clearing heat and detoxicating, swelling and pain relieving；Radix Glycyrrhizae is to make medicine, coordinating the drug actions of a prescription,
With help spleen soil, torr sore myogenic, anti-allergic effects, the blood circulation of sore tissue can be improved and promote the suction of pathological tissues
It receives.
The present invention has the following advantages and effects with respect to the prior art:
(1) present invention is that guidance carries out prescription with Chinese medicine monarch theory, is acted synergistically by plurality of Chinese, from removing
Mite, sterilization, it is anti-inflammatory set about, and achieve the purpose that anti-mite anti-acne in terms of being aided with the comprehensive effect of dispel heat, toxic removing, clearing damp etc..
(2) plant cell wall breaking can be made to have into the cell by the continous way room temperature microjet extraction device that the present invention uses
Effect ingredient dissolves out entirely, realizes effective component high efficiency extraction.Entire Chinese medicine composition preparation process is all to carry out at normal temperature, can be with
Ensure that the activity of effective component keeps reaching best, and energy conservation reaches optimization.
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail, and embodiments of the present invention are not limited thereto.
The test method of specific experiment condition is not specified in the following example, usually according to conventional laboratory conditions or according to system
Make experiment condition proposed by factory.
Propionibacterium acnes used in embodiment (Propionibacterium acnes) are purchased from the triumphant micro- life of Guangdong ring
Object Science and Technology Ltd., model GIM1.162.
A kind of anti-mite anti acne of traditional Chinese medicine composition of embodiment 1-3
A kind of anti-mite anti acne of traditional Chinese medicine composition, Chinese medicinal composition are shown in Table 1.
1 embodiment 1-3 Chinese medicinal composition (in parts by weight) of table
| Traditional Chinese medicinal components | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| Rosemary | 20 | 25 | 18 |
| Dandelion | 20 | 20 | 22 |
| Radix Isatidis | 15 | 18 | 15 |
| Glaucousleaf hydrangeavine stem | 15 | 12 | 15 |
| Paris polyphylla | 15 | 15 | 18 |
| Radix Glycyrrhizae | 15 | 10 | 12 |
Preparation method: after Chinese medicine is crushed, crossing 60 meshes, and mixing is added in traditional extraction tank, and Chinese medicine weight is added
Then the mixed liquor of medicinal material and solvent is passed through continous way room temperature microjet extraction device (ginseng by 10 times of solvent soaking 2 hours
See open file CN107537180A), after centrifugal filtration, filtrate is concentrated into the 1/5 of original volume with film, concentrate macroreticular resin
Absorption, then the ethyl alcohol for being 10-95% with volume fraction repeatedly elute, and collect eluent, and elution liquid film is concentrated into and Chinese medicine phase
Same weight is added and is dissolved with the butanediol of concentrate identical weight, filtering, Chinese medicine composition as provided by the invention.
Comparative example 1-6 single medicinal material extracting solution
Weigh 100 parts of rosemary respectively, 100 parts of dandelion, 100 parts of Radix Isatidis, 100 parts of glaucousleaf hydrangeavine stem, paris polyphylla 100
Part, 100 parts of Radix Glycyrrhizae, number consecutively is comparative example 1-6.Comparative example is successively made respectively according to preparation method described in embodiment 1
The corresponding rosemary of 1-6, dandelion, Radix Isatidis, glaucousleaf hydrangeavine stem, paris polyphylla, Radix Glycyrrhizae extracting solution, i.e., single medicinal material extract
Liquid.
1 anti-mite effect assessment of experimental example
1. subjects
It is 3.0% that the embodiment 1-3 and comparative example 1-6 traditional Chinese medicine composition extract liquid prepared, which is formulated as mass fraction,
Aqueous solution, embodiment 1-3 correspond to test group 1-3, and comparative example 1-6 corresponds to control group 1-6.
2. test method
(1) acarid inhibiting rate is tested
Test group 1-3 and control group 1-6 are tested according to the following steps respectively:
1. being put into one piece of thickness 10mm in the container for having lid, the sponge of side length about 200mm injects suitable saturated brine
(height of water submerges sponge just)；
2. being respectively put into 3 samples and 6 control samples in 9 culture dishes, sample is uniform, smooth, closely laying
In the bottom of culture dish, and on sample, uniformly dispersion is put into 0.05g acarid feed；
3. being put into the acarid of 150 survivals into 9 culture dishes；9 culture dishes are placed on the sponge in container, are trained
It supports the distance between ware and is greater than 10mm；
4. the upper cover of covered container box, culture dish is placed in the incubator of constant temperature and humidity, temperature is (25+2) DEG C, humidity
For (75 ± 5) %；
5. observing and recording the acarid number survived in culture dish with anatomical lens afterwards for 24 hours.
Inhibiting rate Y indicates that B indicates that the average value of three pieces of control sample survival acarid numbers, T indicate three pieces of samples survival acarid numbers
Average value, Y=(B-T)/B*100%, >=90% sample has extremely strong anti-mite effect, and >=75% sample has stronger anti-
Mite effect, >=60% sample have anti-mite effect.
3. test result
Acarid inhibiting rate test result is shown in Table 2.
2 acarid of table inhibits test result
| Test sample | Inhibiting rate |
| Embodiment 1 | 93% |
| Embodiment 2 | 96% |
| Embodiment 3 | 92% |
| Comparative example 1 | 87% |
| Comparative example 2 | 75% |
| Comparative example 3 | 70% |
| Comparative example 4 | 64% |
| Comparative example 5 | 62% |
| Comparative example 6 | 50% |
Embodiment 1-3 shows that Chinese medicine composition prepared by the present invention has obvious suppression to acarid with comparative example 1-6 comparing result
Effect processed, the single medicinal material extracting solution effect than comparative example 1-6 preparation is much better, illustrates to cooperate between traditional Chinese medicinal components after compound
Effect is significant.
Experimental example 2 inhibits propionibacterium acnes efficacy assessments
1, the preparation of Chinese medical extract sample
By embodiment 1-3 and comparative example the 1-6 traditional Chinese medicine composition extract liquid prepared be formulated as mass fraction be 0.5%,
1.0%, 1.5%, 2.0,3.0% aqueous solution, it is spare.
2, to the test method of propionibacterium acnes rejection ability
It respectively takes 6 kinds of sample aqueous solution 1mL to be added separately in sterile glass test tube, topples over the previously prepared improvement of 8mL respectively
GAM broth bouillon (is bought from Qingdao Hai Bo Bioisystech Co., Ltd).Taking-up has divided pure and in 35 DEG C of constant temperature CO2Incubator
The clinical propionibacterium acnes for cultivating 48h take the injection of 1mL bacteria suspension to add the human body culture medium test tube of solution of extract respectively
In, negative control is done with a teat glass without bacteria suspension.Every kind of extract culture medium successively connects since low concentration
Kind propionibacterium acnes, are placed in 35 DEG C of constant temperature CO as early as possible after inoculation2Incubator culture 48h.After culture, human body culture medium is first extracted
In liquid carry out preliminary identification experiment, then by VITEK full automatic microorganism analysis system, ANI Identification of Anaerobic Bacteria card determine
The killing situation of propionibacterium acnes, as a result as the following table 3 is shown ﹕
Table 3 inhibits propionibacterium acnes test result
Embodiment 1-3 shows Chinese medicine composition prepared by the present invention to propionibacterium acnes with comparative example 1-6 comparing result
There is obvious inhibitory effect, the single medicinal material extracting solution effect than comparative example 1-6 preparation is much better, illustrates the Chinese medicine group after compound
It acts synergistically between point significant.
3 irritation evaluation experimental of experimental example
Fermentation liquid irritation is evaluated using rabbit erythrocyte hemolytic experiment.Concrete operations for example Xia ﹕ difference Example 1 to 3 and
The 500 μ L of fermentation liquid of comparative example 1-6 preparation is placed in 2mL centrifuge tube, then is separately added into 2% rabbit erythrocyte suspension, 500 μ L, jog
It mixes, is placed in 37 DEG C of constant incubators after incubating 3h, 2000r/min is centrifuged 3min, and Aspirate supernatant is yin with physiological saline
Property control, using distilled water as positive control, wavelength 414nm at measurement light absorption value (A), haemolysis degree is calculated by formula.
Haemolysis degree=measurement pipe A × (1- hematid specific volume) × 100%/(full haemolysis control tube A × 4)
Wherein, the measuring method of full haemolysis control tube light absorption value (A) is: 2% rabbit erythrocyte suspension being added in 2mL centrifuge tube
1000 μ L, jog mix, and are placed in 37 DEG C of constant incubators after incubating 3h, 2000r/min centrifugation 3min, Aspirate supernatant, with
Physiological saline is negative control, using distilled water as positive control, measures light absorption value (A) at wavelength 414nm.
The results are shown in Table 4.
Table 4
Embodiment 1-3 shows Chinese medicine composition haemolysis ratio of the invention than comparing with the comparison of comparative example 1-6 experimental result
The extracting solution of single medicinal material made from example 1-6 is much lower, and the irritation of extracting solution significantly reduces after compound.
4 anti-mite anti-acne essence of embodiment
A kind of anti-mite anti-acne essence containing anti-mite anti acne of traditional Chinese medicine composition and application, formula are shown in Table 5.
Table 5
Preparation steps:
(1) Sodium Hyaluronate, xanthan gum, hydroxyethyl cellulose are added to evenly dispersed in glycerol, addition EDTA-2Na
And suitable quantity of water, heating stirring keep the temperature 10-20min to 75 DEG C；
(2) butanediol is added and parahydroxyacet-ophenone is dissolved to pellucidity in a heated condition；
(3) mixed solution obtained by (1) is cooled down, and (2) acquired solution is added under agitation, stirring is equal to mixing
It is even；
(4) when the mixed solution obtained by (3) is cooled to 40 DEG C or so, Chinese medicine composition described in embodiment 1 is added, stirs
It is uniformly dissolved and continues to cool down；
(5) essence is uniformly dissolved in advance in PEG-60 rilanit special, is then added to mixed solution obtained by (4)
In, stirring and dissolving is uniform, and formula water is added.
5 anti-mite anti-acne handmade soap of embodiment
A kind of anti-mite anti-acne handmade soap containing anti-mite anti acne of traditional Chinese medicine composition and application, formula are shown in Table 6.
Table 6
Preparation steps:
(1) component A, B component, component C are weighed up in proportion.
(2) component A heating water bath in water-bath is placed in protect after component A heating for dissolving to 80-85 DEG C of stirring and dissolving
Temperature.
(3) B, component C are placed in water-bath and are heated to 60-65 DEG C of heating stirring, then kept the temperature.
(4) B component is poured into component A after mixing evenly, component C is added, mold is quickly poured into when stirring sticky.
The evaluation of 4 anti-acne effect of experimental example
It is young man 30 of 15-30 years old that Ping valence Dui Xiang ﹕, which selects face to have acne, age, wherein male 15, and women 15
Example.
Ping valence Fang Fa ﹕ uses double-blind study, embrocates embodiment 4 sooner or later daily and prepares anti-mite anti-acne essence, 30 days are one
The course for the treatment of.
The quasi- ﹕ of Ping price card 1. 70% or more Xian Xiao ﹕ acne fading；2. 30% or more You Xiao ﹕ acne fading；3. Wu Xiao ﹕ acne
Below fading 30%.
Ping valence Jie Guo ﹕ effective 18, account for 60%；Effective 6, account for 20%；Invalid 6, account for 20%.Total effective rate reaches
80%, illustrate that the present composition has good anti-acne effect.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (10)
1. a kind of anti-mite anti acne of traditional Chinese medicine composition, which is characterized in that the Chinese medicine composition by following parts by weight traditional Chinese medicinal components
5~35 parts of Cheng ﹕ rosemary processed, 5~35 parts of dandelion, 5~35 parts of Radix Isatidis, 5~35 parts of glaucousleaf hydrangeavine stem, paris polyphylla 5~25
Part and 5~25 parts of Radix Glycyrrhizae.
2. anti-mite anti acne of traditional Chinese medicine composition according to claim 1, which is characterized in that the Chinese medicine composition is by following heavy
10~30 parts of rosemary of traditional Chinese medicinal components Cheng ﹕ for measuring part, 10~30 parts of dandelion, 10~30 parts of Radix Isatidis, glaucousleaf hydrangeavine stem 10~30
Part, 10~25 parts and 10~25 parts of Radix Glycyrrhizae of paris polyphylla.
3. according to claim 1 or the preparation method of 2 described in any item anti-mite anti acne of traditional Chinese medicine compositions, it is characterised in that:
Using microjet extraction method, i.e., after Chinese medicine compound prescription mixture being carried out swollen quick-fried extraction, centrifugal filtration, then it is dense with film
Contracting-macroporous resin adsorption-film concentration, obtains Chinese medicine composition；
Specifically comprise the following steps:
1. ingredient: by the removal of impurities of each Chinese medicine material, cleaning, drying, smashing it through 40~100 meshes, mix, it is mixed to obtain Chinese medicine compound prescription
Close object；
2. extracting: Chinese medicine compound prescription mixture and solvent being mixed and impregnated, then by the mixed liquor of Chinese medicine compound prescription mixture and solvent
By continous way room temperature microjet extraction device, precipitating is abandoned in centrifugal filtration, collects filtrate；
3. purification: take filtrate, be concentrated with film, concentrate macroporous resin adsorption, then with volume fraction be 10-95% ethyl alcohol it is more
Secondary elution, collect eluent, will elution liquid film be concentrated into Chinese medicine identical weight, add the fourth two with concentrate identical weight
Alcohol dissolution, is filtered to get anti-mite anti acne of traditional Chinese medicine composition.
4. the preparation method of anti-mite anti acne of traditional Chinese medicine composition according to claim 3, it is characterised in that:
2. middle Chinese medicine compound prescription mixture and solvent press the weight proportion of 1:1~15 to the step, and soaking time is 1~5 hour, described
Solvent is one of water, ethyl alcohol, water-ethanol admixture, 500~3000 turns/min of centrifugal rotational speed；
Step 3. described in filtrate with film be concentrated into the 1/5 of original volume；
The step 1.~3. carry out at room temperature.
5. the described in any item anti-mite anti acne of traditional Chinese medicine compositions of claims 1 or 2 are preparing the application in daily necessities.
6. application according to claim 5, it is characterised in that: anti-mite anti acne of traditional Chinese medicine composition additive amount in the daily necessities
It is based on mass fraction 0.1~25%.
7. a kind of anti-mite anti-acne daily necessities, which is characterized in that contain the described in any item anti-mite anti acne of traditional Chinese medicine of claims 1 or 2
Composition.
8. anti-mite anti-acne daily necessities according to claim 7, which is characterized in that the daily necessities contain with mass percent
It is calculated as 0.1~25% anti-mite anti acne of traditional Chinese medicine composition；
The daily necessities be handmade soap, aqua, type of latex type, cream kind, gel class, powder class, powder compacting class, facial mask, spray-like,
Essence class washes shield class.
9. anti-mite anti-acne daily necessities according to claim 7 or 8, which is characterized in that the daily necessities are anti-mite anti-acne essence
Magnificent liquid, the component including following mass fraction meter:
10. anti-mite anti-acne daily necessities according to claim 7 or 8, which is characterized in that the daily necessities are anti-mite anti-acne hand
Work soap, the component including following mass fraction meter:
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| CN113304085A (en) * | 2021-07-15 | 2021-08-27 | 浙江明洋生命科技有限公司 | Application of plant extract in preparation of cosmetics with antibacterial and mite-killing functions |
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| CN118121516B (en) * | 2024-05-08 | 2024-06-28 | 广州宝仪生物科技有限公司 | Antibacterial acne-removing mite-removing compound and preparation method thereof |
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