CN111631993B

CN111631993B - Composition containing sophora flavescens and magnolia officinalis extracts and cosmetic using composition

Info

Publication number: CN111631993B
Application number: CN202010510141.3A
Authority: CN
Inventors: 龙君; 谭成康
Original assignee: Guangdong Bawei Biotechnology Co ltd
Current assignee: Guangdong Bawei Biotechnology Co ltd
Priority date: 2020-06-08
Filing date: 2020-06-08
Publication date: 2022-04-26
Anticipated expiration: 2040-06-08
Also published as: CN111631993A

Abstract

The invention discloses a composition containing lightyellow sophora root and magnolia bark extracts and a cosmetic using the same, wherein the composition mainly comprises: radix Sophorae Flavescentis extract, cortex Magnolia officinalis extract, herba Artemisiae Annuae extract, semen Oroxyli extract, and dichlorobenzyl alcohol. The composition realizes multi-way mite inhibition through three ways of controlling oil and preventing mites, protecting skin through bacteriostasis and inhibiting mites. In addition, the content of the compound mite removing agent in the composition is low, and the composition is combined with plant mite removing components, so that the product is mild and non-irritant, and meanwhile, the mite removing effect is excellent. The invention discloses a technical scheme, and belongs to the technical field of cosmetics.

Description

Composition containing sophora flavescens and magnolia officinalis extracts and cosmetic using composition

Technical Field

The invention discloses a composition containing sophora flavescens and magnolia bark extracts, in particular to a composition containing sophora flavescens and magnolia bark extracts and a cosmetic applying the composition.

Background

With the continuous improvement of living standard and health consciousness of people, the knowledge and requirements of daily used washing and caring products are also continuously improved. Human Demodex mites, as parasitic mites, are often parasitic in the skin of healthy people, the infection rate of the people reaches 0.8% -91.8%, and the human Demodex mites mainly parasitize parts of the face of the human body, which seriously affect the health and beauty of the people, so the human Demodex mites are widely concerned.

Demodex parasitizing on human body mainly comprises two kinds. Demodex folliculorum parasitizes in areas of the face where sebaceous glands are developed, such as the forehead, nose tip, nasolabial sulcus, cheeks, and forehead. Demodex sebaceus is mainly parasitized at the sebaceous gland ducts and at the gland bottom. Demodex mites of human bodies live on sebum absorption, sebum secretion in adolescence is vigorous, and the mites have enough food to support, can rapidly propagate in large quantities and generate a series of pathological changes. When the human demodex grows, reproduces and metabolizes in hair follicles and sebaceous glands and dead corpses of adults are accumulated to fill pores, the inner diameter of the pores is gradually enlarged, and the pores become coarse. In addition, the propagation of mites easily brings pathogenic microorganisms on the surface of the skin into hair follicles and sebaceous glands, so that the skin is infected, and the symptoms of folliculitis, sebaceous gland inflammation and the like, namely whelk appear. Therefore, it is important to develop products having mite-killing efficacy.

However, in the current cosmetic market, most of mite inhibiting products have a single mite inhibiting way, and all the mite inhibiting way is to remove redundant grease to prevent mites and bacteria from breeding in large quantity as a propaganda point, and part of products have the effects of repairing skin and shrinking pores. However, deep research on the action mechanism of the skin disease caused by mites shows that the aims of preventing and treating the mites by inhibiting grease secretion are far from being sufficient. In addition, physical and pure medicinal mite killing methods are not accepted by most people. In a real sense, the comprehensive acaricidal and acaricidal cosmetics and additives are few and few through various ways, and the development of mild and non-irritant botanical acaricidal products is a future market trend.

Disclosure of Invention

The invention aims to provide a composition containing sophora flavescens and magnolia officinalis extracts and a cosmetic using the composition. The composition realizes multi-way mite inhibition through three ways of controlling oil and preventing mites, protecting skin through bacteriostasis and inhibiting mites, and the product is mild and non-irritant and has an excellent mite removing effect.

Therefore, the first technical scheme provided by the invention is as follows:

a composition containing lightyellow sophora root and magnolia bark extracts is characterized by mainly comprising the following components in parts by weight: radix Sophorae Flavescentis extract, cortex Magnolia officinalis extract, herba Artemisiae Annuae extract, semen Oroxyli extract, and dichlorobenzyl alcohol.

Radix Sophorae Flavescentis, also known as KUGU and CHUANSHEN, is the root of Sophora flavescens ait which is a perennial deciduous shrub plant of Leguminosae. The kuh-seng root contains various alkaloids and is the main active ingredient of the kuh-seng. The radix Sophorae Flavescentis extract has antibacterial effect. In the aspect of inhibiting mites, CN108785212A discloses an anti-mite traditional Chinese medicine composition prepared from various traditional Chinese medicine components including radix sophorae flavescentis, fructus cnidii, radix gentianae and the like.

Magnolia officinalis is a plant of Magnoliaceae and Magnoliaceae, and dried bark, root bark and branch bark of the plant is used as Chinese medicinal material. Magnolia officinalis is pungent in flavor and warm in nature, and has effects of activating qi-flowing, eliminating dampness, warming middle-jiao, relieving pain, lowering adverse qi, and relieving asthma. The Magnolia officinalis decoction has strong antibacterial effect on Staphylococcus, Streptococcus, Bacillus dysenteriae, Pasteurella and Vibrio cholerae.

The sweet wormwood is a sweet wormwood plant of the family Compositae, contains flavonoid, phenolic compounds and terpene compounds with antibacterial and antioxidant effects, and the sweet wormwood extract can promote antibacterial peptide and calprotectin, protect skin from pathogen invasion and promote skin health.

Dichlorobenzyl alcohol is a fungicide, which is commonly used as a preservative in cosmetics. When the content thereof is high, it is liable to cause skin sensitization, because the amount thereof to be used is limited.

According to the invention, dichlorobenzyl alcohol is reasonably combined with the radix sophorae flavescentis extract, the magnolia officinalis extract, the artemisia apiacea extract and the rosewood seed extract, so that an excellent mite removing effect is ensured on the premise of reducing the using amount of dichlorobenzyl alcohol, and the product is mild and has no stimulation.

In the invention, the sophora flavescens extract is preferably extracted from kuh-seng roots, and the magnolia officinalis extract is preferably extracted from magnolia officinalis barks.

In the technical scheme of the invention, the sophora flavescens extract and the magnolia officinalis extract are mainly used for inhibiting and killing mites, and the artemisia apiacea extract is mainly used for inhibiting bacteria to protect skin from being invaded by pathogens. The extract of the seed of rosewood is extracted from the fruit of rosewood, and can reduce the activity of sebaceous glands and reduce the secretion of oil.

In the present invention, a mixture of the extracts of Sophorae radix and Magnolia cortex is preferred, and the mixture is prepared by the following method:

(1) in order to fully dissolve out the effective components of the sophora flavescens and the mangnolia officinalis, the invention firstly carries out enzymolysis on the sophora flavescens and the mangnolia officinalis and fully destroys plant cell walls. Pulverizing dried radix Sophorae Flavescentis and cortex Magnolia officinalis, sieving, adding deionized water according to a material-liquid ratio of 1:20-1:25g/ml, adjusting pH of the solution to 4-5 with HAc-NaAc buffer solution, and adding cellulase with enzyme concentration of 0.3-0.5 mg/ml. The mixed solution is subjected to enzymolysis for 2h at the temperature of 45-50 ℃, and then enzyme deactivation is carried out, so as to obtain the enzymolysis solution of the radix sophorae flavescentis and the mangnolia officinalis.

Preferably, the pulverized particle size of the above radix Sophorae Flavescentis and cortex Magnolia officinalis is 60-100 mesh, such as 60 mesh, 70 mesh, 80 mesh, 90 mesh, 100 mesh, etc.

Preferably, the mass ratio of the radix sophorae flavescentis to the mangnolia officinalis is 5:1-10:1, such as 5:1, 6:1, 7:1, 7.5:1, 8:1, 9:1, 10:1 and the like. The composition extracted in the mass ratio within the range has more excellent mite-proof, sterilization and mite-suppression effects.

Preferably, the enzyme activity of the cellulase is 35000IU/g, and the cell walls of the sophora flavescens and the magnolia officinalis can be fully guaranteed to be broken by enzymolysis under the enzyme activity condition.

(2) Adding a mixed solution of n-butanol and isopropanol which is equal in volume to the deionized water in the step (1) into the enzymolysis solution of the radix sophorae flavescentis and the mangnolia officinalis, performing reflux extraction at 50-60 ℃ for 4 hours, filtering, and collecting filtrate. Concentrating the filtrate to dryness, adding 0.5% hydrochloric acid solution to dissolve, adjusting pH to 1, dissolving for 50-60min, filtering, and collecting hydrochloric acid solution.

Preferably, the volume ratio of n-butanol to isopropanol in the present invention is 1: 4.

(3) And (3) adjusting the hydrochloric acid solution collected in the step (2) to neutral pH, centrifuging and collecting supernatant.

Preferably, the centrifugal speed is 3000-4000r/min, and the centrifugal time is 3-5 min.

(4) And (4) adsorbing the supernatant obtained in the step (3) by using macroporous adsorption resin, eluting for 3 times by using deionized water, then eluting by using an ethanol solution, obtaining an eluent at the flow rate of 6-8BV/h, concentrating the eluent under reduced pressure to be dry, and then freeze-drying to obtain the mixed extract of the sophora flavescens and the magnolia officinalis.

Preferably, the macroporous adsorption resin is AB-8 medium polarity adsorption resin.

Preferably, the ethanol solution has a volume concentration of preferably 20 to 30%, and may be 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, or the like.

According to the invention, the alkaloid content of the mixed extract of the radix sophorae flavescentis and the mangnolia officinalis prepared by the method is higher, the utilization rate of active ingredients in the extract is improved, the mixed extract has a synergistic effect with other components, and the mixed extract has more excellent mite-killing and mite-killing effects, can relieve the skin itch caused by mites, and effectively relieves itching.

In the invention, the preferable materials are 22-30 parts of mixed extracts of radix sophorae flavescentis and mangnolia officinalis, 8-15 parts of sweet wormwood herb extracts, 7-14 parts of rosewood seed extracts and 4-10 parts of dichlorobenzyl alcohol in parts by mass.

Wherein, the mass parts of the mixed extract of the lightyellow sophora root and the officinal magnolia bark can be 22 parts, 22.4 parts, 23 parts, 24 parts, 25 parts, 26 parts, 27 parts, 28 parts, 29 parts, 30 parts and the like.

The sweet wormwood extract can be 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts and the like in parts by mass.

The mass parts of the rosewood seed extract can be 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts and the like.

The dichlorobenzyl alcohol may be present in an amount of 4 parts, 5 parts, 6 parts, 6.8 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.

In the invention, the composition can also comprise 6-30 parts of polyhydric alcohol and 35-54 parts of water by mass.

In the invention, the polyalcohol can be one or a combination of more of propylene glycol, glycerol, butanediol and 1, 2-pentanediol.

In addition, the invention can also be added with some stabilizing additives such as 0.5-2 parts of maltodextrin, 0.5-3 parts of filtrate of fermentation products of leuconostoc/radish root, 0.1-1 part of phenoxyethanol, 0.1-1 part of sodium benzoate and the like.

The preparation method of the composition is to mix and stir various components at normal temperature to obtain the composition.

The invention also provides another technical scheme for the application of the composition in the field of cosmetics. The composition can be added into cosmetic as effective component at mass ratio of 0.1-0.3%. The cosmetic can comprise the composition and a cosmetic matrix, wherein the cosmetic matrix can be one or a combination of more of an emulsifier, a preservative, a pH value regulator, grease, polyalcohol, a skin conditioner, essence, a pigment, a chelating agent, a thickening agent or water.

Compared with the prior art, the technical scheme provided by the invention has the following technical advantages:

(1) the composition provided by the invention is reasonably prepared from the sophora flavescens extract, the magnolia officinalis extract, the artemisia apiacea extract, the rosewood seed extract and dichlorobenzyl alcohol, so that on one hand, the sebaceous gland activity is reduced to control oil secretion, and the food supply required by the growth of mites is blocked to prevent mites; on the other hand, the growth of bacterial microorganisms on the surface of the skin is inhibited, so that the microbial bacteria on the surface of the skin are prevented from being brought into hair follicles and sebaceous glands to cause inflammation when mites multiply; finally, the mites existing in the skin are removed by killing and expelling the mites. The technical scheme provided by the invention can realize multi-way mite inhibition, comprehensively prevent and treat the skin mites from three aspects of oil control, bacteriostasis and mite killing through the synergistic effect of all the components, and has better mite removing effect.

(2) The mixed extract of the radix sophorae flavescentis and the mangnolia officinalis prepared by the method disclosed by the invention is higher in alkaloid content, and after the mixed extract is added into a composition, each component is synergistic, so that the mite removing and sterilizing effects are more excellent. In addition, the composition has a good itching relieving effect, and effectively relieves the skin itching symptom caused by mites.

(3) According to the invention, the plant-type mite-killing active matter is combined with a small amount of compound-type mite-killing components, so that excellent oil-controlling, bacteriostatic and mite-killing effects can be achieved under the condition of low content of the compound-type mite-killing components, and the product is mild and has no stimulation.

Detailed Description

The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. For process parameters or conditions not specifically mentioned, it can be carried out with reference to conventional techniques. The raw materials used in the present invention may be obtained by home-made or purchased from commercial sources. For example, Artemisia annua extract, Leuconostoc/radish root fermentation product filtrate can be obtained from Guangzhou Rongdao chemical Co., Ltd; the extract of the seed of rosewood can be purchased from Xinan chemical trade company of Foshan; dichlorobenzyl alcohol was purchased from Guangdong Dimei New Material science and technology Co.

Example 1

A composition containing lightyellow sophora root and magnolia officinalis extracts comprises the following components in parts by weight: 22 parts of a mixed extract of radix sophorae flavescentis and mangnolia officinalis, 15 parts of a sweet wormwood herb extract, 13 parts of a rosewood seed extract, 6 parts of dichlorobenzyl alcohol, 24 parts of propylene glycol and 40 parts of water.

Wherein, the mixed extract of the radix sophorae flavescentis and the mangnolia officinalis is prepared by the following method:

(1) grinding the sophora flavescens roots and the mangnolia officinalis bark in a mass ratio of 2:9, sieving with a 60-mesh sieve, adding deionized water according to a feed-liquid ratio of 1:20g/ml, adjusting the pH of the solution to 4 by using HAc-NaAc buffer solution, and adding cellulase with enzyme activity of 35000IU/g, wherein the concentration is 0.3 mg/ml. The mixed solution is enzymolyzed for 2h at 45 ℃ and then enzyme is deactivated to obtain an enzymolysis solution of the radix sophorae flavescentis and the mangnolia officinalis.

(2) Adding a mixed solution of n-butanol and isopropanol which is equal in volume to the deionized water in the step (1) into the enzymolysis solution of the radix sophorae flavescentis and the mangnolia officinalis, performing reflux extraction at 50 ℃ for 4 hours, filtering, and collecting filtrate. Wherein the volume ratio of the n-butyl alcohol to the isopropanol is 1: 4. Concentrating the filtrate to dryness, adding 0.5% hydrochloric acid solution to dissolve, adjusting pH to 1, dissolving for 50min, filtering, and collecting hydrochloric acid solution.

(3) Adjusting the pH value of the hydrochloric acid solution collected in the step (2) to be neutral, centrifuging at the rotating speed of 3000r/min for 3min to remove impurities, and collecting supernatant.

(4) And (3) adsorbing the supernatant in the step (3) by using AB-8 medium-polarity macroporous adsorption resin, eluting for 3 times by using deionized water, eluting by using an ethanol solution with the volume concentration of 20%, obtaining an eluent with the flow rate of 6BV/h, concentrating the eluent under reduced pressure to dryness, and freeze-drying to obtain the mixed extract of the sophora flavescens and the magnolia officinalis.

Example 2

A composition containing lightyellow sophora root and magnolia officinalis extracts comprises the following components in parts by weight: 24 parts of mixed extract of radix sophorae flavescentis and mangnolia officinalis, 12 parts of sweet wormwood herb extract, 14 parts of rosewood seed extract, 4 parts of dichlorobenzyl alcohol, 15 parts of butanediol, 10 parts of 1, 2-pentanediol and 41 parts of water.

(1) grinding the sophora flavescens roots and the mangnolia officinalis bark in a mass ratio of 1:1, sieving with a 100-mesh sieve, adding deionized water according to a feed-liquid ratio of 1:25g/ml, adjusting the pH of the solution to 5 by using HAc-NaAc buffer solution, and adding cellulase with enzyme activity of 35000IU/g, wherein the concentration is 0.5 mg/ml. The mixed solution is enzymolyzed at 50 deg.C for 2 hr, and then enzyme is deactivated to obtain enzymolysis solution of radix Sophorae Flavescentis and cortex Magnolia officinalis.

(2) Adding a mixed solution of n-butanol and isopropanol which is equal in volume to the deionized water in the step (1) into the enzymolysis solution of the radix sophorae flavescentis and the mangnolia officinalis, performing reflux extraction at 60 ℃ for 4 hours, filtering, and collecting filtrate. Wherein the volume ratio of the n-butyl alcohol to the isopropanol is 1: 4. Concentrating the filtrate to dryness, adding 0.5% hydrochloric acid solution to dissolve, adjusting pH to 1, dissolving for 60min, filtering, and collecting hydrochloric acid solution.

(3) Adjusting the pH value of the hydrochloric acid solution collected in the step (2) to be neutral, centrifuging at the rotating speed of 4000r/min for 5min to remove impurities, and collecting supernatant.

(4) And (3) adsorbing the supernatant in the step (3) by using AB-8 medium-polarity macroporous adsorption resin, eluting for 3 times by using deionized water, eluting by using an ethanol solution with the volume concentration of 30%, wherein the flow rate of the eluent is 8BV/h to obtain an eluent, concentrating the eluent under reduced pressure until the eluent is dried, and then freeze-drying to obtain the mixed extract of the sophora flavescens and the magnolia officinalis.

Example 3

A composition containing lightyellow sophora root and magnolia officinalis extracts comprises the following components in parts by weight: 30 parts of a mixed extract of radix sophorae flavescentis and mangnolia officinalis, 8 parts of a sweet wormwood herb extract, 10 parts of a rosewood seed extract, 8 parts of dichlorobenzyl alcohol, 5 parts of butanediol, 10 parts of glycerol, 5 parts of propylene glycol, 2 parts of maltodextrin and 42 parts of water.

(1) grinding the sophora flavescens roots and the mangnolia officinalis bark in a mass ratio of 3:2, sieving with a 80-mesh sieve, adding deionized water according to a feed-liquid ratio of 1:23g/ml, adjusting the pH of the solution to 4.5 by using HAc-NaAc buffer solution, and adding cellulase with the enzyme activity of 35000IU/g, wherein the concentration is 0.4 mg/ml. The mixed solution is enzymolyzed for 2h at the temperature of 48 ℃, and then enzyme is deactivated to obtain an enzymolysis solution of the radix sophorae flavescentis and the mangnolia officinalis.

(2) Adding a mixed solution of n-butanol and isopropanol which is equal in volume to the deionized water in the step (1) into the enzymolysis solution of the radix sophorae flavescentis and the mangnolia officinalis, performing reflux extraction at 55 ℃ for 4 hours, filtering, and collecting filtrate. Wherein the volume ratio of the n-butyl alcohol to the isopropanol is 1: 4. Concentrating the filtrate to dryness, adding 0.5% hydrochloric acid solution to dissolve, adjusting pH to 1, dissolving for 55min, filtering, and collecting hydrochloric acid solution.

(3) Adjusting the pH value of the hydrochloric acid solution collected in the step (2) to be neutral, centrifuging at the rotating speed of 3600r/min for 4min to remove impurities, and collecting supernatant.

(4) And (3) adsorbing the supernatant in the step (3) by using AB-8 medium-polarity macroporous adsorption resin, eluting for 3 times by using deionized water, eluting by using an ethanol solution with the volume concentration of 24 percent, obtaining an eluent with the flow rate of 7BV/h, concentrating the eluent under reduced pressure to be dry, and freeze-drying to obtain the mixed extract of the sophora flavescens and the magnolia officinalis.

Example 4

A composition containing lightyellow sophora root and magnolia officinalis extracts comprises the following components in parts by weight: 30 parts of a mixed extract of radix sophorae flavescentis and mangnolia officinalis, 9 parts of a sweet wormwood herb extract, 7 parts of a rosewood seed extract, 10 parts of dichlorobenzyl alcohol, 16 parts of 1, 2-pentanediol, 2 parts of a leuconostoc/radish root fermentation product filtrate and 46 parts of water.

The preparation method of the mixed extract of the radix sophorae flavescentis and the magnolia officinalis is basically the same as that in the embodiment 3, except that the mass ratio of the sophora flavescens root to the magnolia bark in the step (1) is 5: 1.

Example 5

A composition containing lightyellow sophora root and magnolia officinalis extracts comprises the following components in parts by weight: 22 parts of a mixed extract of radix sophorae flavescentis and mangnolia officinalis, 15 parts of a sweet wormwood herb extract, 13 parts of a rosewood seed extract, 6 parts of dichlorobenzyl alcohol, 6 parts of glycerol, 3 parts of a filtrate of a fermentation product of leuconostoc/radish root, 1 part of maltodextrin and 54 parts of water.

The preparation method of the mixed extract of the radix sophorae flavescentis and the magnolia officinalis is basically the same as that in the example 1, except that the mass ratio of the sophora flavescens root to the magnolia bark in the step (1) is 10: 1.

Example 6

A composition containing lightyellow sophora root and magnolia officinalis extracts comprises the following components in parts by weight: 24 parts of mixed extract of radix sophorae flavescentis and mangnolia officinalis, 12 parts of sweet wormwood herb extract, 14 parts of rosewood seed extract, 4 parts of dichlorobenzyl alcohol, 20 parts of glycerol, 10 parts of propylene glycol, 0.5 part of filtrate of fermentation products of leuconostoc/radish root, 0.5 part of maltodextrin and 35 parts of water.

The preparation method of the mixed extract of the radix sophorae flavescentis and the magnolia officinalis is basically the same as that in the example 2, except that the mass ratio of the sophora flavescens root to the magnolia bark in the step (1) is 7: 1.

Comparative example 1

A composition, comprising in parts by weight: 22 parts of a mixed extract of radix sophorae flavescentis and mangnolia officinalis, 28 parts of a sweet wormwood herb extract, 6 parts of dichlorobenzyl alcohol, 24 parts of propylene glycol and 40 parts of water.

Wherein, the preparation method of the mixed extract of the radix sophorae flavescentis and the magnolia officinalis is the same as that of the example 1.

Comparative example 2

A composition, comprising in parts by weight: 24 parts of a mixed extract of radix sophorae flavescentis and mangnolia officinalis, 26 parts of a rosewood seed extract, 4 parts of dichlorobenzyl alcohol, 15 parts of butanediol, 10 parts of 1, 2-pentanediol and 41 parts of water.

Wherein, the preparation method of the mixed extract of the radix sophorae flavescentis and the magnolia officinalis is the same as that of the example 2.

Comparative example 3

A composition, comprising in parts by weight: 15 parts of sweet wormwood herb extract, 13 parts of rosewood seed extract, 28 parts of dichlorobenzyl alcohol, 6 parts of glycerol, 3 parts of a leuconostoc/radish root fermentation product filtrate, 1 part of maltodextrin and 54 parts of water.

Comparative example 4

A composition, comprising in parts by weight: 28 parts of mixed extract of radix sophorae flavescentis and mangnolia officinalis, 12 parts of sweet wormwood herb extract, 14 parts of rosewood seed extract, 20 parts of glycerol, 10 parts of propylene glycol, 0.5 part of filtrate of fermentation products of leuconostoc/radish root, 0.5 part of maltodextrin and 35 parts of water.

Wherein, the preparation method of the mixed extract of the radix sophorae flavescentis and the magnolia officinalis is the same as that of the example 6.

Comparative example 5

A composition, comprising in parts by weight: 4 parts of sophora flavescens root extract, 18 parts of magnolia bark extract, 15 parts of sweet wormwood herb extract, 13 parts of rosewood seed extract, 6 parts of dichlorobenzyl alcohol, 24 parts of propylene glycol and 40 parts of water.

Wherein the extract of radix Sophorae Flavescentis and cortex Magnolia officinalis is obtained from Shanghai Ganoderma Sinense Zhao et Zhang Bio-technology GmbH.

The preparation methods of the compositions described in examples 1-6 and comparative examples 1-5 are to mix and stir the components at room temperature.

To better illustrate the advantages of the compositions of the present invention, the compositions of the present invention were separately subjected to efficacy testing as follows:

1. security patch test

The compositions of the examples 1 to 6 are prepared into aqueous solutions with the mass ratio of 0.3 percent, 20 mu g of the aqueous solutions prepared in the examples are respectively weighed into a spot tester, and meanwhile, a blank control group is arranged, namely water is used as the control. 30 healthy volunteers (11 male and 19 female) are selected, and the patients are 18-30 years old and have no allergic history. The patch test device with the test sample is applied to the curved side of the forearm of the subject with a non-irritating tape, and is applied to the skin uniformly by pressing with the palm for 24 h. The test article was removed after 24 hours, and the skin reaction was observed at 0.5, 24, and 48 hours after patch removal, and the results were recorded according to the skin reaction grading standard in the technical specification for cosmetic safety (2015 edition).

The test results show that the examples 1-6 have no adverse reaction, the subjects have basically negative reaction, and the test samples do not cause adverse reaction to the skin of the subjects in the batch and have high safety according to the regulation in technical Specification for cosmetic safety (2015 edition).

2. Oil control Effect test

Since oil secretion in the skin is involved in androgen metabolism, androgens promote sebaceous gland growth and lipid synthesis, and 5 α -reductase plays a large role therein. The 5 alpha-reductase catalyzes the conversion of testosterone to dihydrotestosterone, which causes many pathological changes when accumulated to high levels in the prostate and skin. In vitro studies show that testosterone and dihydrotestosterone can promote the proliferation of human sebaceous gland cells, is dose-dependent, and has the strongest effect on facial sebaceous gland cells. Thus, the current oil control pathway primarily inhibits sebum secretion by inhibiting 5 α -reductase activity.

To illustrate the oil control effect of the present invention, the present invention investigated the effect of the composition on the inhibition of 5 α -reductase.

Test samples: the compositions of examples 1 to 6 and comparative examples 1 to 5 were prepared into 0.2% aqueous solutions by mass using deionized water, respectively, as test sample solutions. A finasteride solution with the mass ratio of 0.2% is selected as a positive control group.

The test method comprises the following steps: the test sample solution, 50 μ L of positive control group, 100 μ L of testosterone, 200 μ L of Tris buffer solution and 15 μ L of NADPH are added into a reaction tube, 15 μ L of 5 α -reductase is added finally, the A340nm value is determined after mixing, the incubation is carried out at 37 ℃, and the A340nm value is determined after the reaction is carried out for 10 min. Blank reduction (. DELTA.A) was determined by subtracting the background value of the NADPH blank reduction_n). The experiment was repeated three times and the mean value was taken.

100 mu L of testosterone, 200 mu L of Tris buffer solution and 15 mu L of NADPH are added into a reaction tube, 15 mu L of 5 alpha-reductase is finally added, the A340nm value is measured after mixing, the incubation is carried out at 37 ℃, and the A340nm value is measured after the reaction is carried out for 10 min. Blank reduction (. DELTA.A) was determined by subtracting the background value of the NADPH blank reduction₀). The experiment was repeated three times and the mean value was taken.

Wherein, the calculation formula of the inhibition rate is as follows:

I(％)＝(ΔA₀－ΔA_n)/ΔA₀×100％

TABLE 1 oil control Effect of the compositions of the present invention

Sample (I)	5 alpha-reductase inhibition ratio I
		Finasteride solution	64.47％
Example 1 solution	51.58％
		Example 2 solution	53.26％
Example 3 solution	50.39％
		Example 4 solution	57.16％
Example 5 solution	59.23％
		Example 6 solution	60.21％
Comparative example 1 solution	23.67％
		Comparative example 2 solution	42.68％
Comparative example 3 solution	35.12％
		Comparative example 4 solution	38.74％
Comparative example 5 solution	45.48％

Table 1 shows the inhibition of 5 alpha-reductase by the compositions of the present invention and the comparative compositions. As can be seen from the table, the inhibition rate of the composition of the present invention on 5 alpha-reductase can reach more than 50%. In particular, in example 6, the inhibition rate reached 60.21%, which is close to the inhibition effect of the positive control finasteride solution. The inhibiting effect of comparative examples 1 to 5 was only 42.68% at the highest, which was much lower than the inhibiting effect of the composition of the present invention on 5 α -reductase. Therefore, the composition has good effect of inhibiting 5 alpha-reductase, reduces the activity of sebaceous gland cells, reduces oil secretion and achieves good oil control effect.

3. Test of bacteriostatic Effect

The compositions of examples 1 to 6 and comparative examples 1 to 5 of the present invention were subjected to the measurement of antibacterial properties with reference to GB15979-2002 "hygienic Standard for Disposable sanitary articles" appendix C4 "test method for antibacterial Properties of dissolution-type antibacterial (bacteriostatic) product:

(1) preparation of bacterial suspension: and washing the 24h culture of the propionibacterium acnes and the escherichia coli by using 0.03mol/L phosphate buffer solution to prepare 104-106 CFU/mL bacterial suspension.

(2) The compositions of examples 1-6, comparative examples 1-5 were formulated as 0.1% aqueous solutions. Respectively taking 1g of a sample to be tested, adding 0.1mL of the bacterial suspension, uniformly coating and mixing, starting timing, adding 9mL0.03mol/L of phosphate buffer solution after 20min, fully and uniformly mixing, taking 3 dilutions, respectively taking 1mL of the dilutions, placing the dilutions in two parallel plates, adding a corresponding culture medium, and culturing for 24 hours at 36 +/-1 ℃ under an anaerobic condition to count colonies. The above experiment was repeated 3 times, and the average was taken and a blank control group was set.

The calculation formula of the bacteriostatic rate is as follows: and X is (A-B)/A X100%, wherein X is the bacteriostasis rate, A is the average recovered colony of the control sample, and B is the average recovered colony of the test sample.

The evaluation standard of the acne removing efficacy is as follows: (1) the bacteriostatic rate is more than or equal to 90 percent; (2) the bacteriostasis rate is 50-90 percent, which is effective; (3) the bacteriostasis rate is less than 50 percent, which is ineffective.

The evaluation of bacteriostatic efficacy of the skin care products described in examples 1-6 and comparative examples 1-5 is shown in table 1.

Table 2 bacteriostatic test results for the compositions of the invention

Sample (I)	Propionibacterium acnes bacteriostasis rate	Antibacterial rate of Escherichia coli
			Example 1	84.17％	80.26％
Example 2	85.38％	83.78％
			Example 3	82.62％	81.09％
Example 4	90.71％	91.53％
			Example 5	95.23％	93.45％
Example 6	92.24％	92.01％
			Comparative example 1	62.14％	59.30％
Comparative example 2	45.31％	42.54％
			Comparative example 3	50.36％	54.27％
Comparative example 4	48.27％	45.69％
			Comparative example 5	64.04％	60.33％

As can be seen from Table 2, the composition of the invention has the bacteriostatic effect of more than 80 percent and has better bacteriostatic effect. The bacteriostatic effect of the embodiments 4, 5 and 6 can reach 90 percent, and the bacteriostatic rate effect is obvious. The bacteriostasis rate in the comparative example 2 and the comparative example 4 is lower than 50%, and the bacteriostasis is ineffective. Therefore, the bacteriostatic effect of the composition is far better than that of the comparative example.

4. Mite eradication test

The following components in example 1-6 and comparative example 1-5 are added into the following bath formula according to the mass ratio of 0.3 percent: 16% of sodium laureth sulfate, 1% of cocamide DEA, 0.2% of PEG-150 distearate, 4% of disodium laureth sulfosuccinate, 4% of cocamidopropyl betaine, 4% of glycol stearate, 2% of glycerol, 71% of polyquaternary ammonium salt, 0.1% of citric acid, 0.6% of sodium chloride, 0.1% of phenoxyethanol and the balance of water.

Meanwhile, dichlorobenzyl alcohol in a mass ratio of 0.14% was added to the above formulation to make a positive comparative example. And carrying out mite expelling and killing test experiments according to the industry standard NY/T1151.2-2006. Wherein, the test mite species: dust mites (Dermatophagoides farinae), adult and nymphs. The evaluation indexes of the drug effect are shown in a table 3, and the test results of the mite expelling and killing are shown in a table 4.

TABLE 3 evaluation index of drug efficacy

TABLE 4 Deacarid and Deacarid test results

Sample name	Mite-repelling rate	Mite eradication rate
			Shower gel containing example 1	91.53％	95.18％
Shower gel containing example 2	92.39％	97.24％
			Shower gel containing example 3	90.29％	96.35％
Shower gel containing example 4	98.74％	100％
			Shower gel containing example 5	96.56％	100％
Shower gel containing example 6	97.21％	100％
			Bath lotion containing comparative example 1	70.09％	75.46％
Bath lotion containing comparative example 2	70.06％	73.95％
			Bath lotion containing comparative example 3	77.45％	79.37％
Bath lotion containing comparative example 4	60.74％	65.36％
			Bath lotion containing comparative example 5	75.28％	78.36％
Body wash containing dichlorobenzyl alcohol only	99.25％	100％

As can be seen from Table 4, the mite-repellent rate of the shower gel containing the composition is over 90 percent and can reach B level. The mite killing rate of the shower gel containing the composition is over 95 percent and can reach B level. Particularly, after the mixed extract of the radix sophorae flavescentis and the mangnolia officinalis is prepared according to a certain proportion, the alkaloid content is higher, and the composition can achieve a better mite inhibiting effect after being combined with other components, and both the mite expelling and mite killing effects can reach A level, and are equivalent to the mite inhibiting effect of the independently used shower gel containing dichlorobenzyl alcohol. The results in table 4 show that, when an equal amount of the mite-inhibiting active is used, the composition of the present invention can use less compound type mite-inhibiting agent but can also achieve superior mite-killing effect.

The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims

1. A composition containing lightyellow sophora root and officinal magnolia bark extracts is characterized by comprising 22-30 parts of mixed extracts of lightyellow sophora root and officinal magnolia bark, 8-15 parts of sweet wormwood herb extracts, 7-14 parts of rosewood seed extracts and 4-10 parts of dichlorobenzyl alcohol in parts by mass;

the mixed extract of the radix sophorae flavescentis and the mangnolia officinalis is mainly prepared by the following method:

(1) crushing radix sophorae flavescentis and mangnolia officinalis, sieving with a 60-100-mesh sieve, adding deionized water according to a material-liquid ratio of 1:20-1:25g/ml, adjusting the pH of the solution to 4-5 by using HAc-NaAc buffer solution, and adding cellulase with the concentration of 0.3-0.5 mg/ml; performing enzymolysis on the mixed solution at 45-50 deg.C for 2 hr, and inactivating enzyme to obtain enzymolysis solution of radix Sophorae Flavescentis and cortex Magnolia officinalis;

(2) adding a mixed solution of n-butanol and isopropanol which are equal in volume to the deionized water in the step (1) into the enzymolysis solution of the radix sophorae flavescentis and the mangnolia officinalis, performing reflux extraction at 50-60 ℃ for 4 hours, filtering, and collecting filtrate; dissolving and adjusting the pH value of the solution to 1, dissolving for 50-60min, filtering, and collecting hydrochloric acid solution;

(3) adjusting the pH value of the hydrochloric acid solution collected in the step (2) to be neutral, centrifuging at the rotating speed of 3000-4000r/min for 3-5min to remove impurities, and collecting supernatant;

(4) and (3) adsorbing the supernatant obtained in the step (3) by using AB-8 polar macroporous adsorption resin, eluting for 3 times by using deionized water, eluting by using an ethanol solution with the volume concentration of 20-30%, obtaining an eluent with the flow rate of 6-8BV/h, concentrating the eluent under reduced pressure to dryness, and freeze-drying to obtain the mixed extract of the sophora flavescens and the magnolia officinalis.

2. The composition containing Sophorae radix and Magnolia cortex extract as claimed in claim 1, wherein the mass ratio of Sophorae radix to Magnolia cortex in step (1) of the preparation method is 5:1-10: 1.

3. The composition containing sophora flavescens and magnolia officinalis extracts as claimed in claim 1, wherein the enzymatic activity of cellulase in step (1) of the preparation method is 35000IU/g, and the volume ratio of n-butanol to isopropanol in step (2) is 1: 4.

4. The composition of claim 1, wherein the Sophorae radix extract is derived from Sophorae radix and Magnolia cortex extract is derived from Magnolia obovata bark.

5. The composition containing Sophorae radix and Magnolia cortex extract as claimed in claim 1, wherein the composition further comprises 6-30 parts of polyol and 35-54 parts of water.

6. The composition containing Sophorae radix and Magnolia cortex extract as claimed in claim 5, wherein the polyhydric alcohol is one or more of propylene glycol, glycerol, butylene glycol, and 1, 2-pentanediol.

7. The composition containing Sophorae radix and Magnolia cortex extract as claimed in claim 1, wherein the composition containing Sophorae radix and Magnolia cortex extract is added into cosmetic in an amount of 0.1-0.3% by weight.

8. A cosmetic characterized by: the composition containing the sophora flavescens and magnolia officinalis extracts as claimed in any one of claims 1 to 7, and a cosmetic base, wherein the cosmetic base is one or more of an emulsifier, a preservative, a pH regulator, oil and fat, a polyol, a skin conditioner, an essence, a pigment, a chelating agent, a thickener or water.