CN106973892A - A kind of plants essential oil disinfectant for public place - Google Patents
A kind of plants essential oil disinfectant for public place Download PDFInfo
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- CN106973892A CN106973892A CN201710326226.4A CN201710326226A CN106973892A CN 106973892 A CN106973892 A CN 106973892A CN 201710326226 A CN201710326226 A CN 201710326226A CN 106973892 A CN106973892 A CN 106973892A
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- disinfectant
- oil
- tea tree
- tree ethereal
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
Abstract
The present invention relates to a kind of plant source disinfectant, specifically a kind of plants essential oil disinfectant for public place, it contains following component by its volume parts ratio:5 15 parts of tea tree ethereal oil, 5 20 parts of polysorbas20,5 20 parts of polyoxyl 40 hydrogenated castor oil, 10 30 parts of ethanol, the disinfectant has significant bactericidal action, and sterilizing time is short, sterilization of high efficiency, stability is good, has no toxic side effect, and suitable prolonged application is in the sterilization of the public places such as hospital, kindergarten, school, cinema and family.
Description
Technical field
The present invention relates to a kind of plant source disinfectant, specifically a kind of plants essential oil for public place is sterilized
Agent.
Background technology
Disinfectant is used to kill pathogenic microorganism on communication media, reaches innoxious requirement, pathogenic microorganism is disappeared
Go out outside human body, cut off the route of transmission of infectious disease, reach the purpose of control infectious disease.The level that disinfectant is acted on according to it
Bactericidal agent, high effect disinfectants, middle effect disinfectants, low effect disinfectants can be divided into.Present people typically use chemosterilant, make
People gradually recognize chemosterilant while pathogen is killed during, and to the mankind, livestock and poultry, environment, there is also difference
The toxic side effect of degree.Such as, carcinogenic, disinfectant phenolic the excitant of formaldehyde, for drinking water disinfection chlorinated product cause
Mutation effect etc., while the appearance of antibody-resistant bacterium causes Disinfection Effect to decline or fail, therefore, is proposed higher to disinfectant
It is required that.
Under the prospect for focusing on food and Environmental security jointly in the world today, safe and efficient plant source sterilizing is developed
Agent increasingly attracts attention.Chrysanthemum indicum, the capsule of weeping forsythia, Scabrous Mosla Herb, purple are used in the such as patent of the patent No. 201510159682.5
Disinfectant is made in a variety of mixtures such as grass, Radix Angelicae Sinensis, crotons, moutan bark, Lavender, rose hip oil, tea tree ethereal oil, and component is multiple
Miscellaneous, bactericidal effect is not notable, and action time is indefinite;Patent (application number 2014100451136.4) is combined with a variety of Chinese herbal medicines
Staphylococcus aureus, Escherichia coli are had good bactericidal effect by extract, but the chlorophyll of Chinese medical extract is difficult
Remove, decoction color is deeper, easily pollute wall, furniture, clothing etc..
Tea tree ethereal oil (tea tree oil) is extracted from Myrtaceae (Myrtaceae) Melaleuca (Melaleuca)
The fresh branches and leaves of shrub species-narrow leaved tea tree (Melaleuca alternifo-lia).In recent years, tea tree ethereal oil is used as day
Right antiseptic gradually causes the extensive concern of the world of medicine researcher, it was confirmed that tea tree ethereal oil is a kind of has a broad antifungal spectrum, antibacterial work
The property natural antibacterial medicine that strong, clinical efficacy is good, adverse reaction is few.Bacterium is not likely to produce drug resistance to plant extracts, and
Plant extracts toxic side effect is smaller.
Disinfectant is not quite similar with bacteriostatic agent effect, according to《Disinfection technology standard》It is required that, carrier quantitative test and carrier
When flowing soak test, killing logarithm value or inactivation logarithm value >=3.00 of the experiment to each quasi-microorganism, that is, kill micro- life every time
The effect of thing reaches 99.9%.And fungistatic effect, refer to the breeding of energy reversible inhibition bacterium, but do not kill bacterium directly.Typically
Reach that 50% can reach fungistatic effect it is required that suppressing growth of microorganism.Numerous studies, such as cloves, lavender, rosemary and mouse
The essential oils such as tail grass all have good fungistatic effect, but are extremely difficult to bactericidal effect as natural plant product.So, overcome
The deficiencies in the prior art, develop a natural extract disinfectant, meet market and non-chemical the urgent of product disinfectant is essential
Ask, there is important impetus to the development in natural disinfectant agent market.
The content of the invention
The technical problems to be solved by the invention are to overcome the deficiencies in the prior art to have significantly there is provided one kind
Bactericidal action, sterilizing time is short, and bactericidal effect is good, has no toxic side effect, and plants essential oil of the suitable prolonged application in public place disappears
Toxic agent.
The present invention is to be achieved by the following technical programs:
A kind of plants essential oil disinfectant for public place, it is characterised in that:It contains following component by its parts by volume
Number ratio:5-15 parts of tea tree ethereal oil, 5-20 parts of Tween-20,5-20 parts of polyoxyl 40 hydrogenated castor oil, 10-30 parts of ethanol.
Described contains in component, and it presses its volume parts ratio:8 parts of tea tree ethereal oil, 12 parts of Tween-20, polyoxyethylene 40
15 parts of rilanit special, 10 parts of ethanol.
The tea tree ethereal oil that the disinfectant is used is not less than 30% for 4- pine tar alcohol contents, and folium eucalypti oil content is not higher than 3%.
The beneficial effects of the invention are as follows:
This disinfectant has to common pathogens such as staphylococcus aureus, Escherichia coli and Candida albicans significantly to kill
Bacterium effect and stability, acute toxicity test prove that the plants essential oil disinfectant belongs to actual nontoxic level material, preparation method letter
It is single, it is easy to industrialize, be properly applied to the sterilization of the public places such as hospital, kindergarten, school and home environment.
Embodiment
In order that those skilled in the art more fully understand technical scheme, with reference to optimal implementation
The present invention is described in further detail for example.
Embodiment 1
Tea tree ethereal oil 5mL, Tween-20 5mL are mixed, polyoxyl 40 hydrogenated castor oil 10mL is added, is heated to 35-
37 DEG C, stirring makes it fully dissolve.After mixed liquor is cooled to 25 DEG C, 10mL ethanol is added, stirs and evenly mixs and produces.
Embodiment 2
Tea tree ethereal oil 15mL, Tween-20 20mL are mixed, polyoxyl 40 hydrogenated castor oil 20mL is added, is heated to
35-37 DEG C, stirring makes it fully dissolve.After mixed liquor is cooled to 25 DEG C, 30mL ethanol is added, stirs and evenly mixs and produces.
Embodiment 3
Tea tree ethereal oil 8mL, Tween-20 12mL are mixed, polyoxyl 40 hydrogenated castor oil 15mL is added, is heated to 35-
37 DEG C, stirring makes it fully dissolve.After mixed liquor is cooled to 25 DEG C, 10mL ethanol is added, stirs and evenly mixs and produces.
In various embodiments above, the formulation of disinfectant is preferably spray.
The identification of tea tree ethereal oil bactericidal and bacteriostatic effect
(1) experimental method:
Take sterile small plate, indicate inject 10% tea tree ethereal oil solution.By every 5.0mL amount, draw corresponding dense
In the solution injection plate of degree.Disinfectant plate will be filled to put in 20 DEG C of ± 1 DEG C of water baths after 5min, put respectively with aseptic nipper
Enter previously prepared bacterium piece 3, and be allowed to be impregnated with thimerosal.Treat that the interaction of bacterium medicine phases, to each scheduled time, uses sterile tweezer
Bacterium piece is taken out and moved into respectively in a test tube of nertralizer containing 5.0mL by son.20s is mixed with motorized agitator, or by test tube in palm
On shake and strike 80 times, the bacterium on bacterium piece is eluted into neutralizer, then place more than 5min, make neutralization abundant.Most
After further mixing eventually, draw 1.0mL and be directly inoculated with plate, often pipe is inoculated with 2 plates, determine survival bacterium number.
Another to take a plate, injection 10.0mL dilutions replace thimerosal, are put into 2 bacterium pieces, all as positive controls
Test sample is cultivated in 37 DEG C of incubators, and final result is observed to bacterial propagule culture 48h
(2) experimental result:
Bactericidal rate of the tea tree ethereal oil of table 1 10% to golden yellow staphylococcus
Bactericidal rate of the tea tree ethereal oil of table 2 10% to Pseudomonas aeruginosa
Method and the requirement of experiment are killed according to carrier quantitation, the killing rate of each time is >=99.9% or killing logarithm value is equal
>=3.00, it can determine whether that sterilization is qualified.According to result within 30min, 10% tea tree ethereal oil solution does not reach disinfectant sterilization will
Ask.
2. the determination of tea tree ethereal oil sanitizer formulations
The research to the mechanism of action of tea tree ethereal oil antibacterial activity shows at present, and bacterial cell membrane is tea tree ethereal oil effect position
Point.Main mechanism is that the composition in tea tree ethereal oil is inserted between after birth phospholipid molecule, changes its structure, destroys the complete of film
The mobility of whole property and increase film.But this destruction is not to cause whole bacterolysis, but increase after birth is penetrating
Property, cause the ability of important substance leakage and reduction after birth regulation osmotic pressure and discharge harmful substance in bacterium, ultimately result in
Bacterial death.Meanwhile, found according to research, a certain proportion of ethanol can effectively strengthen tea tree ethereal oil to bacteria cell wall
Penetrability, so as to strengthen the combination of essential oil and bacterium, strengthens the function of its restraining and sterilizing bacteria.In addition, the addition of ethanol can be helped
Dissolubility of the tea tree ethereal oil in water, makes tea tree ethereal oil be dissolved in water evenly, strengthens its stability.The present invention is by right
The ethanol of various concentrations is added, a botanical fungicide for meeting bactericide code requirement is developed.
Experimental method:
Sterile small plate is taken, the concentration of injected tea tree ethereal oil and ethanol is indicated.By every 5.0mL amount, draw corresponding
In the solution injection plate of concentration.Disinfectant plate will be filled to put in 20 DEG C of ± 1 DEG C of water baths after 5min, distinguished with aseptic nipper
Previously prepared bacterium piece 3 is put into, and is allowed to be impregnated with thimerosal.The interaction of bacterium medicine phases is treated to each scheduled time, with sterile
Bacterium piece is taken out and moved into respectively in a test tube of nertralizer containing 5.0mL by tweezers.20s is mixed with motorized agitator, or by test tube in hand
Palm, which shakes, to be struck 80 times, the bacterium on bacterium piece is eluted into neutralizer, then places more than 5min, makes neutralization abundant.
After final further mixing, draw 1.0mL and be directly inoculated with plate, often pipe is inoculated with 2 plates, determine survival bacterium number.
Another to take a plate, injection 10.0mL dilutions replace thimerosal, are put into 2 bacterium pieces, all as positive controls
Test sample is cultivated in 37 DEG C of incubators, and final result is observed to bacterial propagule culture 48h
Experimental result:
Bactericidal rate of the ethanol of 3 10% tea tree ethereal oil of table+5% to golden yellow staphylococcus
Bactericidal rate of the ethanol of 4 10% tea tree ethereal oil of table+20% to golden yellow staphylococcus
Bactericidal rate of the ethanol of 5 10% tea tree ethereal oil of table+20% to Escherichia coli
Method and the requirement of experiment are killed according to carrier quantitation, the killing rate of each time is >=99.9% or killing logarithm value is equal
>=3.00, it can determine whether that sterilization is qualified.
Quantitative disinfecting test, acute toxicity test and the stability test of embodiment 3
Experimental method
Bacterium is used in experiment:
Staphylococcus aureus (Staphylococcus aureus) ATCC 6538, Escherichia coli (Escherichia
Coli) 8099, Candida albicans (Candida albicans) ATCC 10231
The preparation of bacterial suspension
According to 2002 editions《Disinfection technology standard》Staphylococcus aureus, Escherichia coli, Candida albicans are inoculated in newly
On fresh nutrient agar, put 37 DEG C and cultivate 24 hours, add the sterile PBS cushioning liquid of 3.0mL~5.0mL and add inclined-plane examination
In pipe, pressure-vaccum, washes lower lawn repeatedly.
The experiment bacteria suspension for preparing various concentrations is standby, bacteria suspension bacteria containing amount 1 × 108cfu/mL~5 × 108cfu/mL
The preparation of bacterium piece
Sterilized 10mm × 10mm filter papers are laid in sterilized petri dishes, whole carrier surface bacterium solution dripping quantity is smoothened
Every is 10 μ l, drips after microbiological contamination liquid, puts and reused after drying in the shade naturally at room temperature.
Nertralizer qualification test
Experimental strain is staphylococcus aureus, totally 6 groups of experiments, is carried out by carrier quantitative germicidal test method.As a result sentence
It is fixed, there are very small amount bacteria growing or asepsis growth with the 1st group, it is many that the 2nd group, which has compared with the 1st group, but be few examination compared with the 3rd, 4,5 (group)
The error rate of bacterial population between bacterium colony growth, the 3rd, 4,5 (group) is tested without departing from 15%, the 6th group of not long bacterium, experiment is repeated 3 times.
Carrier soaks quantitative disinfecting test
Sterile small plate is taken, the concentration of injected thimerosal is indicated.By every 5.0mL amount, disappearing for respective concentration is drawn
In toxic agent solution injection plate.Disinfectant plate will be filled to put in 20 DEG C of ± 1 DEG C of water baths after 5min, put respectively with aseptic nipper
Enter previously prepared bacterium piece 3, and be allowed to be impregnated with thimerosal.Treat that the interaction of bacterium medicine phases, to each scheduled time, uses sterile tweezer
Bacterium piece is taken out and moved into respectively in a test tube of nertralizer containing 5.0mL by son.20s is mixed with motorized agitator, or by test tube in palm
On shake and strike 80 times, the bacterium on bacterium piece is eluted into neutralizer, then place more than 5min, make neutralization abundant.Most
After further mixing eventually, draw 1.0mL and be directly inoculated with plate, often pipe is inoculated with 2 plates, determine survival bacterium number.
Another to take a plate, injection 10.0mL dilutions replace thimerosal, are put into 2 bacterium pieces, all as positive controls
Test sample is cultivated in 37 DEG C of incubators, and final result is observed to bacterial propagule culture 48h;
Acute toxicity test
Selection body weight is 20g Kunming mouses 20, male and female half and half.Preceding overnight fasting is tested, not water restriction.Contamination side
Tested material is given animal by method with oral gavage mode.By the amount of each 0.2mL/10g (body weight), (middle interval is carried out in two times
12h), every mouse is first weighed and fills the disinfectant of respective amount again.The poisoning manifestations and death toll of animal are observed after contamination and dead
Die the time, 14 days observing times.
Stability test
Tea tree ethereal oil disinfectant is the disinfectant using plant as principle active component, therefore microbe method carries out stability
Experiment.By disinfectant solution sealing lucifuge packaging, using accelerated test, it is placed in 54 DEG C of insulating boxs and deposits 14 days, respectively in storage
Forward and backward to be tested by carrier quantitative germicidal test program, each group experiment is repeated 3 times, and is calculated and is killed logarithm value.Killed except determining
It outside microorganism effect, should also observe disinfectant whether there is color change, whether there is precipitation or suspension is produced.
Experimental result
Nertralizer qualification test effect
Nertralizer authentication test results are shown in Table 1.As a result show, nertralizer, which is selected, contains 4% glycine, 2% lecithin, 4%
Glycine, PBS (0.03mol/L) nertralizer can effectively neutralize residual action of the disinfectant to test organisms, and nertralizer and
Neutralized reaction product is on experiment bacteria growing without influence.
The nertralizer authentication test results of table 6
Group | Average colony number (CFU/mL) |
Disinfectant+bacteria suspension | 25 |
Disinfectant+bacteria suspension+nertralizer | 260 |
(disinfectant+nertralizer)+bacteria suspension | 2590000 |
Nertralizer+bacteria suspension | 2750000 |
Bacteria suspension | 2650000 |
Bacterium culture medium control is not inoculated with | 0 |
Quantitative disinfecting test result
Quantitative disinfecting test result shows, disinfectant effect 20min, to staphylococcus aureus, Escherichia coli and white
The average killing logarithm value of color candida albicans >=3 (table 2)
The disinfectant sterilization test result of table 7
Note:It is required that carrier quantitation is killed in experiment, the killing logarithm of each time all should >=3.00, can determine that for sterilization it is qualified.
Experiment is in triplicate
Acute toxicity test
Animal has no obvious poisoning symptom after being administered 14 days, without animal dead during observation, can determine that the disinfectant pair
The oral gavage LD50 of chmice acute is more than 5000mg/kg (body weight), belongs to actual nontoxic level material.
Stability test
Stability test result shows that the disinfectant stock solution is averaged after 54 DEG C preserve 14d to staphylococcus aureus
Kill the basic indifference of logarithm value difference.
The disinfectant of table 8 places the front and rear killing effect to staphylococcus aureus
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (3)
1. a kind of plants essential oil disinfectant for public place, it is characterised in that:It contains following component by its volume parts
Than:5-15 parts of tea tree ethereal oil, 5-20 parts of Tween-20,5-20 parts of polyoxyl 40 hydrogenated castor oil, 10-30 parts of ethanol.
2. a kind of plants essential oil disinfectant for public place according to claim 1, it is characterised in that:Described contains
Have in component, it presses its volume parts ratio:8 parts of tea tree ethereal oil, 12 parts of Tween-20,15 parts of polyoxyl 40 hydrogenated castor oil, second
10 parts of alcohol.
3. a kind of plants essential oil disinfectant for public place according to claim 1 or claim 2, it is characterised in that:The sterilization
The tea tree ethereal oil that agent is used is not less than 30% for 4- pine tar alcohol contents, and folium eucalypti oil content is not higher than 3%.
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107836466A (en) * | 2017-11-10 | 2018-03-27 | 天津中澳嘉喜诺生物科技有限公司 | A kind of tea tree ethereal oil natural disinfectant agent for animals and preparation method thereof |
CN109247343A (en) * | 2018-11-20 | 2019-01-22 | 娇时日化(杭州)股份有限公司 | A kind of thimerosal and preparation method thereof containing tea tree ethereal oil |
CN110583702A (en) * | 2019-09-18 | 2019-12-20 | 禹州市天源生物科技有限公司 | Preparation method of tea tree essential oil disinfectant for animals |
CN110742827A (en) * | 2019-09-27 | 2020-02-04 | 华南师范大学 | Anticorrosive composition and application thereof |
CN114342925A (en) * | 2021-04-08 | 2022-04-15 | 南京和济元生物科技有限公司 | Disinfecting composition and preparation method and application thereof |
CN116440015A (en) * | 2023-02-13 | 2023-07-18 | 大德(浙江)生物科技有限公司 | Plant component disinfectant and self-provided process thereof |
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107836466A (en) * | 2017-11-10 | 2018-03-27 | 天津中澳嘉喜诺生物科技有限公司 | A kind of tea tree ethereal oil natural disinfectant agent for animals and preparation method thereof |
CN109247343A (en) * | 2018-11-20 | 2019-01-22 | 娇时日化(杭州)股份有限公司 | A kind of thimerosal and preparation method thereof containing tea tree ethereal oil |
CN110583702A (en) * | 2019-09-18 | 2019-12-20 | 禹州市天源生物科技有限公司 | Preparation method of tea tree essential oil disinfectant for animals |
CN110742827A (en) * | 2019-09-27 | 2020-02-04 | 华南师范大学 | Anticorrosive composition and application thereof |
CN114342925A (en) * | 2021-04-08 | 2022-04-15 | 南京和济元生物科技有限公司 | Disinfecting composition and preparation method and application thereof |
US11517021B2 (en) | 2021-04-08 | 2022-12-06 | Nanjing Hejiyuan Biological Technology Co., Ltd. | Disinfection composition and preparation method therefor and use thereof |
CN116440015A (en) * | 2023-02-13 | 2023-07-18 | 大德(浙江)生物科技有限公司 | Plant component disinfectant and self-provided process thereof |
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