CN109805038A - A kind of Australia tea tree essential oil disinfection agent and its preparation method and application - Google Patents
A kind of Australia tea tree essential oil disinfection agent and its preparation method and application Download PDFInfo
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- CN109805038A CN109805038A CN201910162929.7A CN201910162929A CN109805038A CN 109805038 A CN109805038 A CN 109805038A CN 201910162929 A CN201910162929 A CN 201910162929A CN 109805038 A CN109805038 A CN 109805038A
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- tea tree
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- australia tea
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Abstract
The present invention provides a kind of Australia tea tree essential oil disinfection agent and its preparation method and application, belong to disinfectant technical field.Australia tea tree essential oil disinfection agent of the present invention, the component including following mass parts: 1~5 part of Australia tea tree essential oil, 20~40 parts of tea tree hydrosol, 0.1~0.5 part of eucalyptus oil, 0.01~0.1 part of Rosemary Oil, 4~10 parts of emulsifier, 10~30 parts of ethyl alcohol and 14.4~64.89 parts of water.Australia tea tree essential oil disinfection agent provided by the invention has significant bactericidal effect and stability to common pathogens such as staphylococcus aureus, pseudomonas aeruginosa and Candida albicans, reach the related request of national disinfectant products, and it has no toxic side effect, noresidue can be used for the multiple fields such as body surface, skin degerming, animal body surface, air environment and pet disinfection.
Description
Technical field
The invention belongs to disinfectant technical fields, and in particular to a kind of Australia tea tree olein disinfectant and preparation method thereof and
Using.
Background technique
Bacterium is an important pathogenic original for causing a large amount of diseases of the mankind, livestock and poultry and pet, for the mankind, micro- life of causing a disease
Object can be propagated by number of ways such as alimentary canal, respiratory tract, skin injury and eye conjunctivas.Report altogether legal in inland of China in 2017
Infectious disease 7,030,879, dead 19,796 people；Wherein for example, bird flu can simultaneously cause death threat to people and poultry,
Morbidity and mortality are high, and the chicken group of infection usually " is annihilated ", are set to category A infectious disease by International Office of Epizootics.Mankind's sense
Majority leads to death with serious pneumonia, a variety of organ failures such as serious person's heart, kidney after dye, and case fatality rate is very high.In addition, such as milk
The disinfection of ox body surface breast and conveyance conduit of milking, cannot get adequate disinfection or has chemical residues, can all lead to dairy products
Quality decline, same problem also often cause food safety and health major reason.With daily sanitizer and beast
With the fast development of sanitizer industry, the disease people of pet caused by bacterium and livestock and poultry are also increasingly paid attention to how having
The sterilization of effect and the upgrowth situation for not influencing animal, become a urgent problem to be solved.
Disinfectant is generally used for killing pathogenic microorganism on communication media, innoxious requirement is reached, by the micro- life of cause of disease
Object is eliminated except human body, and the route of transmission of infectious disease is cut off, and achievees the purpose that control infectious disease.It generally uses in the prior art
Chemosterilant, for chemosterilant while killing pathogen, to the mankind, livestock and poultry, environment, there is also not in use
With the toxic side effect of degree, while usually generating drug resistance and the problems such as medicament residue.For example, the carcinogenic of formaldehyde, phenols disinfection
The irritation of agent, for mutagenesis of chlorinated product of drinking water disinfection etc., while the appearance of antibody-resistant bacterium causes disinfection to be imitated
Fruit decline or failure.Therefore, overcome the deficiencies in the prior art develops a natural extract disinfectant, meets market pair
The urgent need of non-chemical product disinfectant has important impetus to the development in natural disinfectant agent market.
Summary of the invention
In view of this, the purpose of the present invention is to provide a kind of Australia tea tree essential oil disinfection agent and preparation method thereof and answering
With.Australia tea tree essential oil disinfection agent provided by the invention is to staphylococcus aureus, pseudomonas aeruginosa and Candida albicans etc.
Common pathogen has significant bactericidal effect and stability, and has no toxic side effect, noresidue.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of Australia tea tree essential oil disinfection agent, the component including following mass parts: Australia tea tree essential oil 1
~5 parts, 20~40 parts of tea tree hydrosol, 0.1~0.5 part of eucalyptus oil, 0.01~0.1 part of Rosemary Oil, 4~10 parts of emulsifier,
10~30 parts and 14.4~64.89 parts of water of ethyl alcohol.
Preferably, 4- terpinol and 1,8- cineole, the matter of the 4- terpinol are contained in the Australia tea tree essential oil
Measure percentage composition >=30%, mass percentage≤5% of 1, the 8- cineole.
Preferably, australene, mass percentage >=20% of the australene are contained in the Rosemary Oil.
Preferably, the emulsifier includes one or more of Cremophor RH40, glycerol and Tween-80.
The present invention provides the preparation methods of Australia tea tree essential oil disinfection agent described in above-mentioned technical proposal, including following step
It is rapid:
(1) the Australia tea tree essential oil, eucalyptus oil, Rosemary Oil and emulsifier are mixed, obtains the first mixed material；
(2) the first mixed material that the step (1) obtains is mixed with ethyl alcohol, obtains the second mixed material；
(3) the second mixed material that the step (2) obtains is mixed with tea tree hydrosol, obtains third mixed material；
(4) the third mixed material that the step (3) obtains is mixed with water, obtains Australia tea tree essential oil disinfection agent.
Preferably, the pH value for the Australia tea tree essential oil disinfection agent that the step (4) obtains is 5.4~5.6.
The present invention also provides application of the Australia tea tree essential oil disinfection agent in skin degerming described in above-mentioned technical proposal.
The present invention also provides Australia tea tree essential oil disinfection agent answering in surface disinfection described in above-mentioned technical proposal
With.
The present invention also provides the answering in air environment disinfection of Australia tea tree essential oil disinfection agent described in above-mentioned technical proposal
With.
The present invention also provides the answering in the disinfection of animal body surface of Australia tea tree essential oil disinfection agent described in above-mentioned technical proposal
With.
Australia tea tree essential oil disinfection agent provided by the invention, the ingredient in Australia tea tree essential oil are inserted into after birth phospholipid molecule
Between, change its structure, destroys the integrality of film and increase the mobility of film.But this destruction is not to cause entirely carefully
Bacterium dissolution, but increase the permeability of after birth, cause important content leaks in bacterium and reduce after birth adjusting osmotic pressure and row
The ability of harmful substance out, eventually leads to bacterial death.Australia tea tree essential oil, eucalyptus oil, tea tree hydrosol and fan of the present invention
Repeatedly essential oil synergistic effect can effectively enhance Australia tea tree essential oil to the penetrability of bacteria cell wall, thus enhance essential oil with
The combination of bacterium enhances the function of its restraining and sterilizing bacteria.
The embodiment of the present invention is as the result is shown: Australia tea tree essential oil disinfection agent provided by the invention, and 5min kills golden yellow
The killing rate of staphylococcus, pseudomonas aeruginosa and Candida albicans is >=99.999%.
Specific embodiment
The present invention provides a kind of Australia tea tree essential oil disinfection agent, the component including following mass parts:
1~5 part of Australia tea tree essential oil, 20~40 parts of tea tree hydrosol, 0.1~0.5 part of eucalyptus oil, Rosemary Oil 0.01~
0.1 part, 4~10 parts of emulsifier, 10~30 parts of ethyl alcohol and 14.4~64.89 parts of water.
Australia tea tree essential oil disinfection agent provided by the invention include mass parts be 1~5 part of Australia tea tree essential oil, preferably 2
~4 parts, more preferably 3 parts.The present invention is not particularly limited the source of the Australia tea tree essential oil, it is preferred to use Australia Australia
Tea tree ethereal oil and hydrosol are purchased in Tianjin Zhong Aojia Xi Nuo Biotechnology Co., Ltd.
In the present invention, the Australia tea tree essential oil preferably comprises the 4- terpinol and quality of mass percentage >=30%
The 1,8- cineole of percentage composition≤5%.
In the present invention, the ingredient in the Australia tea tree essential oil is inserted between after birth phospholipid molecule, changes it
Structure destroys the integrality of film and increases the mobility of film；This destruction can increase the permeability of after birth, cause bacterium
Interior important content leaks and the ability for reducing after birth adjusting osmotic pressure and discharge harmful substance, eventually lead to bacterial death.
Australia tea tree essential oil disinfection agent provided by the invention include mass parts be 20~40 parts of tea tree hydrosols, preferably 25~
35 parts, more preferably 30 parts.The present invention is not particularly limited the source of the tea tree hydrosol, it is preferred to use Australia tea tree is pure
Dew, is purchased in Tianjin Zhong Aojia Xi Nuo Biotechnology Co., Ltd.
In the present invention, the tea tree hydrosol has the function of sterilization and lasting.
Australia tea tree essential oil disinfection agent provided by the invention include mass parts be 0.1~0.5 part of eucalyptus oil, preferably 0.2
~0.4 part, more preferably 0.3 part.The present invention is not particularly limited the source of the eucalyptus oil, preferably conforms to GB
1886.33-2015 requirement.
In the present invention, the eucalyptus oil has mould proof, sterilization and corrosion-resistant effect, can enhance Australia tea tree essential oil
Sterilizing ability, and there is potent transdermal rush infiltration effect.
Australia tea tree essential oil disinfection agent provided by the invention includes that mass parts are 0.01~0.1 part of Rosemary Oil, preferably
It is 0.02~0.08 part, more preferably 0.05 part.The present invention is not particularly limited the source of the Rosemary Oil, preferably accords with
Close the requirement of ISO 1342-2012.
In the present invention, the Rosemary Oil preferably comprises the australene of mass percentage >=20%.
In the present invention, the Rosemary Oil has the function of mould proof and antibacterial, can enhance Australia tea tree essential oil
Sterilizing ability.
Australia tea tree essential oil disinfection agent provided by the invention include mass parts be 4~10 parts of emulsifiers, preferably 5~8 parts,
More preferably 6 parts.The present invention is not particularly limited the type of the emulsifier, preferably Cremophor RH40, glycerol
One or more of with Tween-80；When the emulsifier is two kinds, preferably to wait mass ratioes to be mixed；When the cream
When agent is three kinds, the mass ratio of Cremophor RH40, glycerol and Tween-80 is preferably 2:1:2.The present invention is to the cream
The source of agent is not particularly limited, using conventional commercial product.
In the present invention, the emulsifier can be such that oily matter is uniformly distributed in water, form stable solution.
Australia tea tree essential oil disinfection agent provided by the invention include mass parts be 10~30 parts of ethyl alcohol, preferably 15~25
Part, more preferably 20 parts.The present invention is not particularly limited the type of the ethyl alcohol, preferably dehydrated alcohol.The present invention is to described
The source of ethyl alcohol is not particularly limited, using conventional commercial product.
In the present invention, the ethyl alcohol plays dissolution assistant effect, facilitates the dissolubility of Australia tea tree essential oil in water, makes Australia
Continent tea tree ethereal oil dissolves more evenly in water, enhances its stability；Meanwhile there is synergy in terms of sterilization with Australia tea tree essential oil
Effect.
Australia tea tree essential oil disinfection agent provided by the invention include mass parts be 14.4~64.89 parts of water, preferably 15~
60 parts, more preferably 30 parts.In the present invention, the water is preferably deionized water.
The present invention also provides the preparation method of the Australia tea tree essential oil disinfection agent described in above-mentioned technical proposal, including it is following
Step:
(1) the Australia tea tree essential oil, eucalyptus oil, Rosemary Oil and emulsifier are mixed, obtains the first mixed material；
(2) the first mixed material that the step (1) obtains is mixed with ethyl alcohol, obtains the second mixed material；
(3) the second mixed material that the step (2) obtains is mixed with tea tree hydrosol, obtains third mixed material；
(4) the third mixed material that the step (3) obtains is mixed with water, obtains Australia tea tree essential oil disinfection agent.
The present invention mixes the Australia tea tree essential oil, eucalyptus oil, Rosemary Oil and emulsifier, obtains the first mixture
Material.The present invention is not particularly limited the mixed mode, using conventional mixing method.
The present invention mixes the first obtained mixed material with ethyl alcohol, obtains the second mixed material.The present invention is to institute
The mode for stating mixing is not particularly limited, it is preferred to use the method for stirring, the time of the stirring are preferably 10~20min, more
Preferably 15min.
The present invention mixes the second obtained mixed material with tea tree hydrosol, obtains third mixed material.The present invention
The mixed mode is not particularly limited, it is preferred to use the method for stirring stirs to clarify transparency liquid.
The present invention mixes the obtained third mixed material with water, obtains Australia tea tree essential oil disinfection agent.In this hair
In bright, the pH value of the Australia tea tree essential oil disinfection agent is preferably 5.4~5.6, and more preferably 5.5.It is described to adjust the molten of pH value
The citric acid solution that liquid preferred mass score is 50%.
The present invention is not particularly limited the dosage form of the Australia tea tree essential oil disinfection agent, preferably spray.
In the present invention, object is preferably directly sprayed or be applied to the application method of the Australia tea tree essential oil disinfection agent
Surface.
The present invention also provides application of the Australia tea tree essential oil disinfection agent in skin degerming described in above-mentioned technical proposal.
The present invention also provides Australia tea tree essential oil disinfection agent answering in surface disinfection described in above-mentioned technical proposal
With.In the present invention, the body surface preferably includes cultivation colony house and equipment surface.
The present invention also provides the answering in air environment disinfection of Australia tea tree essential oil disinfection agent described in above-mentioned technical proposal
With.
The present invention also provides the answering in the disinfection of animal body surface of Australia tea tree essential oil disinfection agent described in above-mentioned technical proposal
With.
Below in conjunction with the embodiment in the present invention, technical solution provided by the invention is clearly and completely described.
Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based in the present invention
Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts, all
Belong to the scope of protection of the invention.
Embodiment 1
Australia tea tree essential oil 1.0%, Australia tea tree hydrosol 20.0%, eucalyptus oil 0.1%, Rosemary Oil 0.01%,
Cremophor RH40 6.0%, dehydrated alcohol 10.0% and water 62.89%.By above-mentioned Australia tea tree essential oil, eucalyptus oil, fan
Repeatedly essential oil and Cremophor RH40 are sufficiently mixed, and are in uniform micro white liquid, are obtained the first mixed material；Above-mentioned
Ethyl alcohol is added in one mixed material, to dissolving, mixing time 15min obtains the second mixed material for stirring；In above-mentioned second mixing
Australia tea tree hydrosol is added in material, is stirred until homogeneous, is in clear liquid, obtains third mixed material；By above-mentioned third
Mixed material is mixed with water, with 50% aqueous citric acid solution, is adjusted pH to 5.5 and is sufficiently stirred, and (stands one day without layering, discharging
Up to Australia tea tree essential oil disinfection agent.
The identification of 1 disinfectant bactericidal and bacteriostatic effect of embodiment
(1) experimental method:
Sterile small plate is taken, indicates injected solution.By the amount of every 5.0mL, the disinfectant of extraction embodiment 1 injects
In plate.Disinfectant plate will be filled and set 5min in 20 DEG C of water baths, be respectively put into previously prepared bacterium piece 3 with aseptic nipper
Piece, and be allowed to be impregnated in thimerosal.To bacterium and thimerosal interaction 5min, the taking-up of bacterium piece is moved into respectively with aseptic nipper
In one test tube of neutralizer containing 5.0mL.20s is mixed with motorized agitator, or test tube is shaken on palm and is struck 80 times, makes bacterium on piece
Bacterium is eluted into neutralizer, then places 5min, keeps neutralization abundant.After final further mixing, it is straight to draw 1.0mL
Inoculation plate, every pipe are inoculated with 2 plates, measurement survival bacterium number.The neutralization agent prescription are as follows: 3% Tween-80+0.5% is thio
+ 0.1% cysteine of+1.43% lecithin of sodium sulphate+0.5%L- histidine+0.85% sodium chloride of+0.5% peptone.
A plate is separately taken, injection 10.0mL aseptic deionized water replaces thimerosal, 2 bacterium pieces is put into, as positive control
All test samples of group are cultivated in 37 DEG C of incubators, observe final result to bacterial propagule culture 48h.
(2) experimental result:
1 embodiment of table, 1 disinfectant is in 5min to the bactericidal rate of microorganism
It is required according to " disinfection technology standard ", carrier quantitative test, every time killing logarithm of the test to each quasi-microorganism
Or inactivation logarithm >=3.00, the i.e. effect of destroying microorganisms reach 99.9%.
As can be seen from Table 1, the disinfectant of the embodiment of the present invention 1 is to staphylococcus aureus, pseudomonas aeruginosa and white
The killing rate of color candida albicans >=99.999%, can determine whether that disinfection is qualified.The disinfectant of embodiment 1 reaches sterilization and requires.
Embodiment 2
Australia tea tree essential oil 2.0%, Australia tea tree hydrosol 30.0%, eucalyptus oil 0.3%, Rosemary Oil 0.01%,
Cremophor RH40 4.0%, Tween-80 4.0%, dehydrated alcohol 30.0% and water 29.69%.By above-mentioned Australia tea tree essence
Oil, eucalyptus oil, Rosemary Oil, Cremophor RH40 and Tween-80 are sufficiently mixed, and are in uniform micro white liquid, are obtained
First mixed material；Ethyl alcohol is added in above-mentioned first mixed material, to dissolving, it is mixed to obtain second by mixing time 15min for stirring
Close material；Australia tea tree hydrosol is added in above-mentioned second mixed material, is stirred until homogeneous, is in clear liquid, obtains the
Three mixed materials；Above-mentioned third mixed material is mixed with water, with 50% aqueous citric acid solution, pH to 5.5 is adjusted and is sufficiently stirred,
One day is stood without layering, is discharged up to the agent of Australia tea tree essential oil disinfection.
The identification of 2 disinfectant bactericidal and bacteriostatic effect of embodiment
(1) experimental method:
Sterile small plate is taken, indicates injected solution.By the amount of every 5.0mL, the disinfectant of extraction embodiment 2 injects
In plate.Disinfectant plate will be filled and set 5min in 21 DEG C of water baths, be respectively put into previously prepared bacterium piece 3 with aseptic nipper
Piece, and be allowed to be impregnated in thimerosal.To bacterium and thimerosal interaction 5min, the taking-up of bacterium piece is moved into respectively with aseptic nipper
In one test tube of neutralizer containing 5.0mL.20s is mixed with motorized agitator, or test tube is shaken on palm and is struck 80 times, makes bacterium on piece
Bacterium is eluted into neutralizer, then places 5min, keeps neutralization abundant.After final further mixing, it is straight to draw 1.0mL
Inoculation plate, every pipe are inoculated with 2 plates, measurement survival bacterium number.The neutralization agent prescription are as follows: 3% Tween-80+0.5% is thio
+ 0.1% cysteine of+1.43% lecithin of sodium sulphate+0.5%L- histidine+0.85% sodium chloride of+0.5% peptone.
A plate is separately taken, injection 10.0mL aseptic deionized water replaces thimerosal, 2 bacterium pieces is put into, as positive control
All test samples of group are cultivated in 37 DEG C of incubators, observe final result to bacterial propagule culture 48h.
(2) experimental result:
2 embodiment of table, 2 disinfectant is in 5min to the bactericidal rate of microorganism
It is required according to " disinfection technology standard ", carrier quantitative test, every time killing logarithm of the test to each quasi-microorganism
Or inactivation logarithm >=3.00, the i.e. effect of destroying microorganisms reach 99.9%.
As can be seen from Table 2, the disinfectant of the embodiment of the present invention 2 is to staphylococcus aureus, pseudomonas aeruginosa and white
The killing rate of color candida albicans >=99.999%, can determine whether that disinfection is qualified.The disinfectant of embodiment 2 reaches sterilization and requires.
Embodiment 3
Australia tea tree essential oil 4.0%, Australia tea tree hydrosol 20.0%, eucalyptus oil 0.5%, Rosemary Oil 0.05%,
Cremophor RH40 6.0%, Tween-80 4.0%, dehydrated alcohol 25.0% and water 40.45%.By above-mentioned Australia tea tree essence
Oil, eucalyptus oil, Rosemary Oil, Cremophor RH40 and Tween-80 are sufficiently mixed, and are in uniform micro white liquid, are obtained
First mixed material；Ethyl alcohol is added in above-mentioned first mixed material, to dissolving, it is mixed to obtain second by mixing time 15min for stirring
Close material；Australia tea tree hydrosol is added in above-mentioned second mixed material, is stirred until homogeneous, is in clear liquid, obtains the
Three mixed materials；Above-mentioned third mixed material is mixed with water, with 50% aqueous citric acid solution, pH to 5.5 is adjusted and is sufficiently stirred,
One day is stood without layering, is discharged up to the agent of Australia tea tree essential oil disinfection.
The identification of 3 disinfectant bactericidal and bacteriostatic effect of embodiment
(1) experimental method:
Sterile small plate is taken, indicates injected solution.By the amount of every 5.0mL, the disinfectant of extraction embodiment 3 injects
In plate.Disinfectant plate will be filled and set 5min in 21 DEG C of water baths, be respectively put into previously prepared bacterium piece 3 with aseptic nipper
Piece, and be allowed to be impregnated in thimerosal.To bacterium and thimerosal interaction 5min, the taking-up of bacterium piece is moved into respectively with aseptic nipper
In one test tube of neutralizer containing 5.0mL.20s is mixed with motorized agitator, or test tube is shaken on palm and is struck 80 times, makes bacterium on piece
Bacterium is eluted into neutralizer, then places 5min, keeps neutralization abundant.After final further mixing, it is straight to draw 1.0mL
Inoculation plate, every pipe are inoculated with 2 plates, measurement survival bacterium number.The neutralization agent prescription are as follows: 3% Tween-80+0.5% is thio
+ 0.1% cysteine of+1.43% lecithin of sodium sulphate+0.5%L- histidine+0.85% sodium chloride of+0.5% peptone.
A plate is separately taken, injection 10.0mL aseptic deionized water replaces thimerosal, 2 bacterium pieces is put into, as positive control
All test samples of group are cultivated in 37 DEG C of incubators, observe final result to bacterial propagule culture 48h.
(2) experimental result:
3 embodiment of table, 3 disinfectant is in 5min to the bactericidal rate of microorganism
It is required according to " disinfection technology standard ", carrier quantitative test, every time killing logarithm of the test to each quasi-microorganism
Or inactivation logarithm >=3.00, the i.e. effect of destroying microorganisms reach 99.9%.
As can be seen from Table 3, the disinfectant of the embodiment of the present invention 3 is to staphylococcus aureus, pseudomonas aeruginosa and white
The killing rate of color candida albicans >=99.999%, can determine whether that disinfection is qualified.The disinfectant of embodiment 3 reaches sterilization and requires.
Embodiment 4
Australia tea tree essential oil 5.0%, Australia tea tree hydrosol 40.0%, eucalyptus oil 0.5%, Rosemary Oil 0.1%, PEG-
40 rilanit specials 4.0%, Tween-80 4.0%, glycerol 2.0, dehydrated alcohol 30.0% and water 14.4%.By above-mentioned Australia tea
Tree essential oil, eucalyptus oil, Rosemary Oil, Cremophor RH40 and Tween-80 are sufficiently mixed, and are in uniform micro white liquid,
Obtain the first mixed material；It is added ethyl alcohol in above-mentioned first mixed material, stirring is to dissolving, mixing time 15min, obtains the
Two mixed materials；Australia tea tree hydrosol is added in above-mentioned second mixed material, is stirred until homogeneous, is in clear liquid, obtains
To third mixed material；Above-mentioned third mixed material is mixed with water, with 50% aqueous citric acid solution, it is abundant to adjust pH to 5.5
Stirring stands one day without layering, discharges up to the agent of Australia tea tree essential oil disinfection.
The identification of 4 disinfectant bactericidal and bacteriostatic effect of embodiment
(1) experimental method:
Sterile small plate is taken, indicates injected solution.By the amount of every 5.0mL, the disinfectant of extraction embodiment 4 injects
In plate.Disinfectant plate will be filled and set 5min in 21 DEG C of water baths, be respectively put into previously prepared bacterium piece 3 with aseptic nipper
Piece, and be allowed to be impregnated in thimerosal.It interacts to bacterium and thimerosal to 5min, is moved the taking-up of bacterium piece respectively with aseptic nipper
Enter in a test tube of neutralizer containing 5.0mL.20s is mixed with motorized agitator, or test tube is shaken on palm and is struck 80 times, makes bacterium on piece
Bacterium be eluted into neutralizer, then place 5min, keep neutralization abundant.After final further mixing, 1.0mL is drawn
It is directly inoculated with plate, every pipe is inoculated with 2 plates, measurement survival bacterium number.The neutralization agent prescription are as follows:+0.5% sulphur of 3% Tween-80
+ 0.1% cysteine of+1.43% lecithin of sodium thiosulfate+0.5%L- histidine+0.85% sodium chloride of+0.5% peptone.
A plate is separately taken, injection 10.0mL aseptic deionized water replaces thimerosal, 2 bacterium pieces is put into, as positive control
All test samples of group are cultivated in 37 DEG C of incubators, observe final result to bacterial propagule culture 48h.
(2) experimental result:
4 embodiment of table, 4 disinfectant is in 5min to the bactericidal rate of microorganism
It is required according to " disinfection technology standard ", carrier quantitative test, every time killing logarithm of the test to each quasi-microorganism
Or inactivation logarithm >=3.00, the i.e. effect of destroying microorganisms reach 99.9%.
As can be seen from Table 4, the disinfectant of the embodiment of the present invention 4 is to staphylococcus aureus, pseudomonas aeruginosa and white
The killing rate of color candida albicans >=99.999%, can determine whether that disinfection is qualified.The disinfectant of embodiment 4 reaches sterilization and requires.
Embodiment 5
To the disinfectant of the embodiment 1 of sterilizing ability qualification, live skin antiseptic field experiment, experimental data are carried out
It is as shown in table 5:
The experimental data that the disinfectant of 5 embodiment 1 of table carries out disinfection to skin
As shown in Table 5, positive controls have more bacterial growth.To be killed to 30 person-times batches of being averaged for skin surface natural bacteria
It goes out logarithm >=1.00, it is qualified that disinfection can be judged to.The disinfectant of embodiment 1 is significantly larger than requirement of experiment in skin sterilization level.
As can be seen from the above embodiments, Australia tea tree essential oil disinfection agent provided by the invention, 5min kill golden yellow Portugal
The killing rate of grape coccus, pseudomonas aeruginosa and Candida albicans is >=99.999%, false single to staphylococcus aureus, verdigris
The common pathogens such as born of the same parents bacterium and Candida albicans have significant bactericidal effect and stability, and have no toxic side effect, noresidue.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of Australia tea tree essential oil disinfection agent, which is characterized in that the component including following mass parts:
1~5 part of Australia tea tree essential oil, 20~40 parts of tea tree hydrosol, 0.1~0.5 part of eucalyptus oil, Rosemary Oil 0.01~0.1
Part, 4~10 parts of emulsifier, 10~30 parts of ethyl alcohol and 14.4~64.89 parts of water.
2. Australia tea tree essential oil disinfection agent according to claim 1, which is characterized in that contain in the Australia tea tree essential oil
4- terpinol and 1,8- cineole, mass percentage >=30% of the 4- terpinol, the matter of 1, the 8- cineole
Measure percentage composition≤5%.
3. Australia tea tree essential oil disinfection agent according to claim 1, which is characterized in that contain α-in the Rosemary Oil
Firpene, mass percentage >=20% of the australene.
4. Australia tea tree essential oil disinfection agent according to claim 1, which is characterized in that the emulsifier includes PEG-40 hydrogen
Change one or more of castor oil, glycerol and Tween-80.
5. the preparation method of Australia tea tree essential oil disinfection agent described in Claims 1 to 4 any one, which is characterized in that including
Following steps:
(1) the Australia tea tree essential oil, eucalyptus oil, Rosemary Oil and emulsifier are mixed, obtains the first mixed material；
(2) the first mixed material that the step (1) obtains is mixed with ethyl alcohol, obtains the second mixed material；
(3) the second mixed material that the step (2) obtains is mixed with tea tree hydrosol, obtains third mixed material；
(4) the third mixed material that the step (3) obtains is mixed with water, obtains Australia tea tree essential oil disinfection agent.
6. preparation method according to claim 5, which is characterized in that the Australia tea tree essential oil that the step (4) obtains disappears
The pH value of toxic agent is 5.4~5.6.
7. the agent of Australia tea tree essential oil disinfection or claim 5~6 described in Claims 1 to 4 any one are described in any item
Application of the Australia tea tree essential oil disinfection agent that preparation method is prepared in skin degerming.
8. the agent of Australia tea tree essential oil disinfection or claim 5~6 described in Claims 1 to 4 any one are described in any item
Application of the Australia tea tree essential oil disinfection agent that preparation method is prepared in surface disinfection.
9. the agent of Australia tea tree essential oil disinfection or claim 5~6 described in Claims 1 to 4 any one are described in any item
Application of the Australia tea tree essential oil disinfection agent that preparation method is prepared in air environment disinfection.
10. the agent of Australia tea tree essential oil disinfection or claim 5~6 described in Claims 1 to 4 any one are described in any item
Application of the Australia tea tree essential oil disinfection agent that preparation method is prepared in the disinfection of animal body surface.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910162929.7A CN109805038A (en) | 2019-03-05 | 2019-03-05 | A kind of Australia tea tree essential oil disinfection agent and its preparation method and application |
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| CN201910162929.7A CN109805038A (en) | 2019-03-05 | 2019-03-05 | A kind of Australia tea tree essential oil disinfection agent and its preparation method and application |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110583702A (en) * | 2019-09-18 | 2019-12-20 | 禹州市天源生物科技有限公司 | Preparation method of tea tree essential oil disinfectant for animals |
| CN111214398A (en) * | 2020-03-19 | 2020-06-02 | 潍坊富邦药业有限公司 | Clear and transparent water-soluble tea tree essential oil and preparation method thereof |
| CN111568809A (en) * | 2020-05-08 | 2020-08-25 | 张惠如 | Preparation method and application of water-based safe plant antibacterial agent |
| CN114342964A (en) * | 2021-12-21 | 2022-04-15 | 广东长康科技有限公司 | Bactericide and preparation method and application thereof |
| CN115554335A (en) * | 2022-03-29 | 2023-01-03 | 广州果熊生物科技有限公司 | Mother and infant protective essential oil and preparation and use method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102036691A (en) * | 2008-04-24 | 2011-04-27 | 桑尼维普斯Pty有限公司 | Disinfecting formulation |
| CN102239893A (en) * | 2011-05-04 | 2011-11-16 | 云南同方科技有限公司 | Plant essential oil bactericidal disinfectant |
| CN105854056A (en) * | 2016-03-30 | 2016-08-17 | 云南悦馨香料科技有限公司 | Air freshener with sterilization function, and preparation method thereof |
| CN106342946A (en) * | 2016-08-02 | 2017-01-25 | 中山圣菲环保科技有限公司 | Air purification disinfectant and method for preparing same |
| CN106818917A (en) * | 2016-12-15 | 2017-06-13 | 苏州市启扬商贸有限公司 | A kind of disinfected fragrant agent |
| CN106906049A (en) * | 2017-04-12 | 2017-06-30 | 四川艾朗特生物科技有限公司 | A kind of Australia tea tree hydrosol refining technique |
| CN107836466A (en) * | 2017-11-10 | 2018-03-27 | 天津中澳嘉喜诺生物科技有限公司 | A kind of tea tree ethereal oil natural disinfectant agent for animals and preparation method thereof |
-
2019
- 2019-03-05 CN CN201910162929.7A patent/CN109805038A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102036691A (en) * | 2008-04-24 | 2011-04-27 | 桑尼维普斯Pty有限公司 | Disinfecting formulation |
| CN102239893A (en) * | 2011-05-04 | 2011-11-16 | 云南同方科技有限公司 | Plant essential oil bactericidal disinfectant |
| CN105854056A (en) * | 2016-03-30 | 2016-08-17 | 云南悦馨香料科技有限公司 | Air freshener with sterilization function, and preparation method thereof |
| CN106342946A (en) * | 2016-08-02 | 2017-01-25 | 中山圣菲环保科技有限公司 | Air purification disinfectant and method for preparing same |
| CN106818917A (en) * | 2016-12-15 | 2017-06-13 | 苏州市启扬商贸有限公司 | A kind of disinfected fragrant agent |
| CN106906049A (en) * | 2017-04-12 | 2017-06-30 | 四川艾朗特生物科技有限公司 | A kind of Australia tea tree hydrosol refining technique |
| CN107836466A (en) * | 2017-11-10 | 2018-03-27 | 天津中澳嘉喜诺生物科技有限公司 | A kind of tea tree ethereal oil natural disinfectant agent for animals and preparation method thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110583702A (en) * | 2019-09-18 | 2019-12-20 | 禹州市天源生物科技有限公司 | Preparation method of tea tree essential oil disinfectant for animals |
| CN111214398A (en) * | 2020-03-19 | 2020-06-02 | 潍坊富邦药业有限公司 | Clear and transparent water-soluble tea tree essential oil and preparation method thereof |
| CN111568809A (en) * | 2020-05-08 | 2020-08-25 | 张惠如 | Preparation method and application of water-based safe plant antibacterial agent |
| CN114342964A (en) * | 2021-12-21 | 2022-04-15 | 广东长康科技有限公司 | Bactericide and preparation method and application thereof |
| CN115554335A (en) * | 2022-03-29 | 2023-01-03 | 广州果熊生物科技有限公司 | Mother and infant protective essential oil and preparation and use method thereof |
| CN115554335B (en) * | 2022-03-29 | 2023-11-21 | 广州果熊生物科技有限公司 | Protective essential oil and preparation method thereof |
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