CN114342964A - Bactericide and preparation method and application thereof - Google Patents
Bactericide and preparation method and application thereof Download PDFInfo
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- CN114342964A CN114342964A CN202111577306.XA CN202111577306A CN114342964A CN 114342964 A CN114342964 A CN 114342964A CN 202111577306 A CN202111577306 A CN 202111577306A CN 114342964 A CN114342964 A CN 114342964A
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- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 82
- 239000003899 bactericide agent Substances 0.000 title claims abstract description 75
- 238000002360 preparation method Methods 0.000 title claims abstract description 31
- 239000000341 volatile oil Substances 0.000 claims abstract description 55
- 235000009024 Ceanothus sanguineus Nutrition 0.000 claims abstract description 41
- 235000015459 Lycium barbarum Nutrition 0.000 claims abstract description 41
- 239000000203 mixture Substances 0.000 claims abstract description 39
- 240000000233 Melia azedarach Species 0.000 claims abstract description 36
- 239000010642 eucalyptus oil Substances 0.000 claims abstract description 36
- 229940044949 eucalyptus oil Drugs 0.000 claims abstract description 36
- 241000746375 Andrographis Species 0.000 claims abstract description 11
- 240000003553 Leptospermum scoparium Species 0.000 claims abstract 9
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 74
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 52
- 238000003756 stirring Methods 0.000 claims description 30
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 23
- 239000012752 auxiliary agent Substances 0.000 claims description 16
- 239000003921 oil Substances 0.000 claims description 15
- WEEGYLXZBRQIMU-UHFFFAOYSA-N Eucalyptol Chemical compound C1CC2CCC1(C)OC2(C)C WEEGYLXZBRQIMU-UHFFFAOYSA-N 0.000 claims description 13
- WRYLYDPHFGVWKC-UHFFFAOYSA-N 4-terpineol Chemical compound CC(C)C1(O)CCC(C)=CC1 WRYLYDPHFGVWKC-UHFFFAOYSA-N 0.000 claims description 12
- 239000000417 fungicide Substances 0.000 claims description 12
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- 229920000053 polysorbate 80 Polymers 0.000 claims description 9
- WRYLYDPHFGVWKC-SNVBAGLBSA-N 4-Terpineol Natural products CC(C)[C@]1(O)CCC(C)=CC1 WRYLYDPHFGVWKC-SNVBAGLBSA-N 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 4
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- 230000000855 fungicidal effect Effects 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 1
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- 230000000052 comparative effect Effects 0.000 description 18
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- NIXOWILDQLNWCW-UHFFFAOYSA-N Acrylic acid Chemical compound OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 4
- 229920002125 Sokalan® Polymers 0.000 description 4
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 4
- 229960001631 carbomer Drugs 0.000 description 4
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- 229950008882 polysorbate Drugs 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 2
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
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- RFFOTVCVTJUTAD-UHFFFAOYSA-N cineole Natural products C1CC2(C)CCC1(C(C)C)O2 RFFOTVCVTJUTAD-UHFFFAOYSA-N 0.000 description 1
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- 239000010681 turmeric oil Substances 0.000 description 1
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Abstract
The invention belongs to the technical field of sterilization and disinfection, and discloses a bactericide as well as a preparation method and application thereof. The bactericide comprises herba Andrographitis extract, fructus Toosendan extract, tea tree essential oil, and oleum Eucalypti. The bactericide contains common andrographis herb extract, chinaberry seed extract, tea tree essential oil and eucalyptus oil, and the common andrographis herb extract, chinaberry seed extract, tea tree essential oil and eucalyptus oil have synergistic bacteriostasis and sterilization effects, and the sterilization effect is better when the components are matched according to a specific ratio. Can effectively dissolve and destroy the bacterial biofilm so as to achieve the purposes of disinfection and environment cleaning. And because the components are all from natural plants, the composition is mild to human bodies, is not easy to generate bacterial drug resistance and is environment-friendly.
Description
Technical Field
The invention belongs to the technical field of sterilization and disinfection, and particularly relates to a bactericide as well as a preparation method and application thereof.
Background
The concept of health and hygiene of sterilization and disinfection is gradually deepened. When selecting and using the sterilization and disinfection products, people not only pay attention to the sterilization and disinfection effects of the products, but also pay attention to the mild effect of the products on human bodies. The product is required to have high-efficiency sterilization and disinfection effects, and is also required to have good mildness to human bodies.
The current common disinfectant can be divided into chlorine-containing disinfectant, peroxide disinfectant, alcohol (such as ethanol) disinfectant and other disinfectant according to components. Various bacteria or viruses exist in the environment where people live, most microorganisms in the air die in a short time due to lack of nutrition and sunlight radiation, but once the microorganisms are combined with carriers such as water vapor, dust and the like to form a biological film, the biological film can resist the influence of the external adverse environment, so that the bacteria can better survive. The existing environment sterilization disinfectant mainly comprises chemical components, such as 84 disinfectant, hypochlorous acid, hydrogen peroxide disinfectant, chlorine, quicklime, ethanol and the like. The odor is usually large, some of the odor is bad in mildness to human bodies and harmful, and the odor needs to be ventilated for a long time after the odor is used, so that the odor has poor practicability in practical application. The long-term use of these disinfectants not only causes the generation of bacterial resistance, but also causes environmental pollution.
Therefore, it is urgently needed to provide a new sterilization and disinfection agent which not only has good sterilization effect, but also is mild to human body, is not easy to generate bacterial drug resistance, and is environment-friendly.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art described above. Therefore, the bactericide has a good sterilizing effect, is mild to a human body, is not easy to generate bacterial drug resistance, and is environment-friendly.
The invention conception of the invention is as follows: the bactericide contains an andrographis paniculata extract, a chinaberry seed extract, tea tree essential oil and eucalyptus oil, and the synergistic bacteriostasis and sterilization effects of the andrographis paniculata extract, the chinaberry seed extract, the tea tree essential oil and the eucalyptus oil exist at the same time. And because the components are all from natural plants, the composition is mild to human bodies, is not easy to generate bacterial drug resistance and is environment-friendly.
In a first aspect, the present invention provides a biocide.
Specifically, the bactericide comprises an andrographis paniculata extract, a chinaberry seed extract, tea tree essential oil and eucalyptus oil.
Preferably, the tea tree essential oil is of a cineole type and comprises 4-terpineol and 1, 8-cineole, wherein the mass percent of the 4-terpineol is more than or equal to 38%, and the mass percent of the 1, 8-cineole is less than or equal to 5%; further preferably, the components comprise 4-terpineol and 1, 8-cineole, wherein the mass percent of the 4-terpineol is more than or equal to 38 percent, and the mass percent of the 1, 8-cineole is less than or equal to 3 percent.
Preferably, the bactericide further comprises an auxiliary agent; further preferably, the auxiliary agent comprises at least one of tween-80, ethanol, glycerol or water.
Preferably, the bactericide comprises 0.1-8 parts by weight of common andrographis herb extract, 0.01-8 parts by weight of chinaberry seed extract, 0.01-20 parts by weight of tea tree essential oil and 0.01-8 parts by weight of eucalyptus oil.
More preferably, the bactericide comprises 0.1-5 parts by weight of common andrographis herb extract, 0.01-5 parts by weight of chinaberry seed extract, 0.01-15 parts by weight of tea tree essential oil and 0.01-5 parts by weight of eucalyptus oil.
Preferably, the bactericide further comprises, by weight, tween-800.1 to 35 parts; further preferably, the bactericide further comprises, by weight, tween-800.1 to 30 parts; more preferably tween-802-20 parts.
Preferably, the bactericide further comprises 800.1-5 parts by weight of glycerol; further preferably, the bactericide further comprises 0.1-3 parts by weight of glycerol; more preferably 1-2 parts of glycerol.
Preferably, the bactericide further comprises 4-35 parts by weight of ethanol; further preferably, the bactericide further comprises 5-30 parts by weight of ethanol; more preferably 8-20 parts of ethanol.
Preferably, the bactericide further comprises 7-95 parts by weight of water; more preferably, the bactericide further comprises 20-60 parts by weight of water.
Preferably, the bactericide comprises, by weight, 0.1-5 parts of an andrographis paniculata extract, 0.01-5 parts of a chinaberry seed extract, 0.01-15 parts of tea tree essential oil, 0.01-5 parts of eucalyptus oil, 800.1-30 parts of tween-800.1, 0.1-3 parts of glycerol and 5-30 parts of ethanol.
More preferably, the bactericide comprises, by weight, 0.1-5 parts of an andrographis paniculata extract, 0.01-5 parts of a chinaberry seed extract, 0.01-15 parts of tea tree essential oil, 0.01-5 parts of eucalyptus oil, 800.1-30 parts of tween-800.1, 0.1-3 parts of glycerol, 5-30 parts of ethanol and 20-60 parts of water. The proper amount of each component is selected, so that the bactericide has better stability and can play a bactericidal role uniformly and long-term.
In a second aspect, the invention provides a method for preparing a bactericide.
In particular to a preparation method of a bactericide, which is used for preparing the bactericide.
Preferably, the preparation method of the bactericide comprises the following steps:
(1) mixing tea tree essential oil and eucalyptus oil to obtain an essential oil mixture;
(2) mixing tween-80, ethanol and glycerol to prepare an auxiliary agent mixture, and then mixing the auxiliary agent mixture with the essential oil mixture prepared in the step (1) to prepare emulsified oil;
(3) and (3) adding the common andrographis herb extract, the chinaberry seed extract and water into the emulsified oil prepared in the step (2), and stirring and mixing to obtain the bactericide.
Preferably, in the step (3), the stirring speed is 500-; further preferably, the stirring speed is 500-600 rpm. High speed stirring is helpful to prepare uniform and stable bactericide.
Preferably, in the step (3), the stirring time is 8-18 minutes; further preferably, the stirring time is 10 to 15 minutes.
A third aspect of the invention provides a use of a biocide.
The bactericide is used in preparing bactericidal, antiviral and antifungal agents.
Compared with the prior art, the invention has the following beneficial effects:
the bactericide contains an andrographis paniculata extract, a chinaberry seed extract, tea tree essential oil and eucalyptus oil, and the simultaneous existence of the andrographis paniculata extract, the chinaberry seed extract, the tea tree essential oil and the eucalyptus oil has a synergistic antibacterial and bactericidal effect, and the bactericidal effect is better when the components are matched according to a specific ratio. Can effectively dissolve and destroy the bacterial biofilm so as to achieve the purposes of disinfection and environment cleaning. And because the components are all from natural plants, the composition is mild to human bodies, is not easy to generate bacterial drug resistance and is environment-friendly.
Detailed Description
In order to make the technical solutions of the present invention more apparent to those skilled in the art, the following examples are given for illustration. It should be noted that the following examples are not intended to limit the scope of the claimed invention.
The starting materials, reagents or apparatuses used in the following examples are conventionally commercially available or can be obtained by conventionally known methods, unless otherwise specified.
The tea tree essential oil used in the following examples is also known as MELALEUCA ALTERNIFOLIA (MELALEUCA ALTERNIFOLIA) leaf oil, purchased from nanning wanghui perfumery ltd.
Example 1: preparation of fungicides
A bactericide comprises, by weight, 0.1 part of an extract of Andrographis paniculata Nees, 5 parts of an extract of Melia azedarach seeds, 12.5 parts of tea tree essential oil, and 1 part of eucalyptus oil.
A preparation method of a bactericide comprises the following steps:
the raw material components are stirred and mixed, the stirring speed is 200 revolutions per minute, and the stirring time is 10 minutes, so that the bactericide is prepared.
Example 2: preparation of fungicides
A bactericide comprises, by weight, 3.5 parts of an extract of Andrographis paniculata Nees, 1 part of an extract of Melia azedarach seeds, 3.5 parts of tea tree essential oil, and 3.5 parts of eucalyptus oil.
A preparation method of a bactericide comprises the following steps:
the raw material components are stirred and mixed, the stirring speed is 200 revolutions per minute, and the stirring time is 10 minutes, so that the bactericide is prepared.
Example 3: preparation of fungicides
A bactericide comprises (by weight parts) herba Andrographitis extract 2.5, fructus Toosendan extract 2.5, tea tree essential oil 7.5, and oleum Eucalypti 2.5.
A preparation method of a bactericide comprises the following steps:
the raw material components are stirred and mixed, the stirring speed is 200 revolutions per minute, and the stirring time is 10 minutes, so that the bactericide is prepared.
Example 4: preparation of fungicides
A bactericide comprises, by weight, 1 part of an extract of Andrographis paniculata Nees, 3.5 parts of an extract of Melia azedarach seeds, 15 parts of tea tree essential oil, and 0.01 part of eucalyptus oil.
A preparation method of a bactericide comprises the following steps:
the raw material components are stirred and mixed, the stirring speed is 200 revolutions per minute, and the stirring time is 10 minutes, so that the bactericide is prepared.
Example 5: preparation of fungicides
A bactericide comprises, by weight, 5 parts of an extract of Andrographis paniculata Nees, 0.01 part of an extract of Melia azedarach seeds, 0.01 part of tea tree essential oil, and 5 parts of eucalyptus oil.
A preparation method of a bactericide comprises the following steps:
the raw material components are stirred and mixed, the stirring speed is 200 revolutions per minute, and the stirring time is 10 minutes, so that the bactericide is prepared.
Example 6: preparation of fungicides
A bactericide comprises, by weight, 0.1 part of an andrographis paniculata extract, 5 parts of a chinaberry seed extract, 12.5 parts of tea tree essential oil, 1 part of eucalyptus oil, 805 parts of tween-glycerol, 1.5 parts of glycerol, 20 parts of ethanol and 54.9 parts of water.
A preparation method of a bactericide comprises the following steps:
(1) mixing tea tree essential oil and eucalyptus oil to obtain an essential oil mixture;
(2) mixing tween-80, ethanol and glycerol to prepare an auxiliary agent mixture, and then mixing the auxiliary agent mixture with the essential oil mixture prepared in the step (1) to prepare emulsified oil;
(3) and (3) adding the common andrographis herb extract, the chinaberry seed extract and water into the emulsified oil prepared in the step (2), stirring and mixing at the stirring speed of 600 revolutions per minute for 10 minutes, and preparing the bactericide.
Example 7: preparation of fungicides
A bactericide comprises, by weight, 3.5 parts of an andrographis paniculata extract, 1 part of a chinaberry seed extract, 3.5 parts of tea tree essential oil, 3.5 parts of eucalyptus oil, 800.1 parts of tween-800.1 parts, 2 parts of glycerol, 30 parts of ethanol and 56.4 parts of water.
A preparation method of a bactericide comprises the following steps:
(1) mixing tea tree essential oil and eucalyptus oil to obtain an essential oil mixture;
(2) mixing tween-80, ethanol and glycerol to prepare an auxiliary agent mixture, and then mixing the auxiliary agent mixture with the essential oil mixture prepared in the step (1) to prepare emulsified oil;
(3) and (3) adding the common andrographis herb extract, the chinaberry seed extract and water into the emulsified oil prepared in the step (2), stirring and mixing at the stirring speed of 600 revolutions per minute for 10 minutes, and preparing the bactericide.
Example 8: preparation of fungicides
A bactericide comprises, by weight, 2.5 parts of an extract of Andrographis paniculata Nees, 2.5 parts of an extract of Melia azedarach seeds, 7.5 parts of tea tree essential oil, 2.5 parts of eucalyptus oil, 5 parts of Tween-8015 parts of glycerol, 15 parts of ethanol and 54.5 parts of water.
A preparation method of a bactericide comprises the following steps:
(1) mixing tea tree essential oil and eucalyptus oil to obtain an essential oil mixture;
(2) mixing tween-80, ethanol and glycerol to prepare an auxiliary agent mixture, and then mixing the auxiliary agent mixture with the essential oil mixture prepared in the step (1) to prepare emulsified oil;
(3) and (3) adding the common andrographis herb extract, the chinaberry seed extract and water into the emulsified oil prepared in the step (2), stirring and mixing at the stirring speed of 550 revolutions per minute for 12 minutes, and preparing the bactericide.
Example 9: preparation of fungicides
A bactericide comprises, by weight, 1 part of an extract of Andrographis paniculata Nees, 3.5 parts of an extract of Melia azedarach seeds, 15 parts of tea tree essential oil, 0.01 part of eucalyptus oil, 20 parts of Tween-8020 parts of glycerol, 1 part of ethanol, and 56.49 parts of water.
A preparation method of a bactericide comprises the following steps:
(1) mixing tea tree essential oil and eucalyptus oil to obtain an essential oil mixture;
(2) mixing tween-80, ethanol and glycerol to prepare an auxiliary agent mixture, and then mixing the auxiliary agent mixture with the essential oil mixture prepared in the step (1) to prepare emulsified oil;
(3) and (3) adding the common andrographis herb extract, the chinaberry seed extract and water into the emulsified oil prepared in the step (2), stirring and mixing at the stirring speed of 500 revolutions per minute for 15 minutes, and preparing the bactericide.
Example 10: preparation of fungicides
A bactericide comprises, by weight, 5 parts of an andrographis paniculata extract, 0.01 part of a chinaberry seed extract, 0.01 part of tea tree essential oil, 5 parts of eucalyptus oil, 8030 parts of tween-8030 parts of glycerol, 5 parts of ethanol and 54.88 parts of water.
A preparation method of a bactericide comprises the following steps:
(1) mixing tea tree essential oil and eucalyptus oil to obtain an essential oil mixture;
(2) mixing tween-80, ethanol and glycerol to prepare an auxiliary agent mixture, and then mixing the auxiliary agent mixture with the essential oil mixture prepared in the step (1) to prepare emulsified oil;
(3) and (3) adding the common andrographis herb extract, the chinaberry seed extract and water into the emulsified oil prepared in the step (2), stirring and mixing at the stirring speed of 600 revolutions per minute for 10 minutes, and preparing the bactericide.
Comparative example 1
Compared with example 2, comparative example 1 is different only in that eucalyptus oil is replaced with the same amount of extract of roots of sophora flavescens ait, and the rest of the components and the preparation process are the same as example 2.
Comparative example 2
Comparative example 2 is different from example 2 only in that the same amount of zedoary turmeric oil is used instead of tea tree essential oil, and the remaining components and preparation process are the same as example 2.
Comparative example 3
Compared with example 2, comparative example 3 is different only in that the same amount of biota orientalis extract is used instead of the chinaberry seed extract, and the rest of the components and the preparation process are the same as example 2.
Comparative example 4
Comparative example 4 is different from example 2 only in that the same amount of scutellaria extract is used instead of andrographis paniculata extract, and the remaining components and preparation process are the same as example 2.
Comparative example 5
Weighing 10g of andrographis paniculata extract, dissolving the andrographis paniculata extract in a propylene glycol solvent to obtain a disinfection composition mixed solution with the concentration of 10 wt%, adding the disinfection composition mixed solution into a disinfectant matrix (the disinfectant matrix comprises 67.7% of water, 15% of carbomer, 1.5% of glycerol, 10% of propylene glycol, 805% of polysorbate and 0.8% of triethanolamine) according to the mass fraction of 10%, and uniformly stirring to prepare the disinfectant.
Comparative example 6
Weighing 10g of chinaberry seed extract, dissolving the chinaberry seed extract by using a propylene glycol solvent to obtain a disinfection composition mixed solution with the concentration of 10 wt%, adding the disinfection composition mixed solution into a disinfectant matrix (the disinfectant matrix comprises 67.7% of water, 15% of carbomer, 1.5% of glycerol, 10% of propylene glycol, 805% of polysorbate and 0.8% of triethanolamine) according to the mass fraction of 10%, and uniformly stirring to prepare the disinfectant.
Comparative example 7
Weighing 10g of tea tree essential oil, dissolving the tea tree essential oil with a propylene glycol solvent to obtain a disinfection composition mixed solution with the concentration of 10 wt%, adding the disinfection composition mixed solution into a disinfectant matrix (the disinfectant matrix comprises 67.7% of water, 15% of carbomer, 1.5% of glycerol, 10% of propylene glycol, 805% of polysorbate and 0.8% of triethanolamine) according to the mass fraction of 10%, and uniformly stirring to prepare the disinfectant.
Comparative example 8
Weighing 10g of eucalyptus oil, dissolving the eucalyptus oil in a propylene glycol solvent to obtain a disinfection composition mixed solution with the concentration of 10 wt%, adding the disinfection composition mixed solution into a disinfectant matrix (the disinfectant matrix comprises 67.7% of water, 15% of carbomer, 1.5% of glycerol, 10% of propylene glycol, 805% of polysorbate and 0.8% of triethanolamine) according to the mass fraction of 10%, and uniformly stirring to prepare the disinfectant.
Comparative example 9
75% by volume of ethanol.
Comparative example 10
84 sterilizing liquid.
Product effectiveness testing
The bactericides prepared in examples 1 to 6, the bactericides prepared in comparative examples 1 to 8, the 75% ethanol and the 84 disinfectant of comparative examples 9 to 10 were subjected to a sterilization test.
In the experiment, staphylococcus aureus ATCC 6538, escherichia coli 8099, pseudomonas aeruginosa ATCC 15442 and candida albicans 8032 are selected as experimental strains, and sterilization experiments are carried out on the self-prepared bacterial suspensions, wherein 1mL of the self-prepared bacterial suspensions are respectively and uniformly coated by 1 × 105-1×106cfu/ml of a plate solid culture medium of a bacterial solution, wherein a physiological saline solution is dripped into one plate solid culture medium to serve as a blank control. The change in the number of bacterial colonies before and after the experiment was observed after 5min and recorded. Reference for specific experimental operationThe technical specification of disinfection effect test of disinfection products in 2002 edition 'disinfection technical specification', and the experimental results of the disinfectant microbicidal test are shown in table 1.
TABLE 1
As can be seen from Table 1, the bactericidal effects of examples 1-6 of the present invention are significantly better than those of comparative examples 1-8, wherein the bactericidal effects of examples 3-4 are the best and equivalent to that of 84 disinfectant. And is milder, green and environment-friendly compared with 75% alcohol and 84 disinfectant, and is nontoxic and harmless to human bodies. As can be seen from the bactericidal effects of examples 1-6 and comparative examples 1-8 of the present invention, the andrographis paniculata extract, melia azedarach seed extract, tea tree essential oil and eucalyptus oil in the bactericide of the present invention cannot be replaced by other substances, and the andrographis paniculata extract, melia azedarach seed extract, tea tree essential oil and eucalyptus oil have synergistic effects.
Claims (10)
1. A bactericide is characterized by comprising an andrographis paniculata extract, a chinaberry seed extract, tea tree essential oil and eucalyptus oil.
2. The bactericide as claimed in claim 1, wherein the tea tree essential oil comprises 4-terpineol and 1, 8-cineole, wherein the mass percentage of 4-terpineol is not less than 38%, and the mass percentage of 1, 8-cineole is not more than 5%.
3. The bactericide according to claim 1, which further comprises an auxiliary.
4. The bactericide as claimed in claim 3, wherein the auxiliary comprises at least one of Tween-80, ethanol, glycerol or water.
5. The fungicide according to claim 1, which comprises 0.1-8 parts by weight of andrographis paniculata extract, 0.01-8 parts by weight of melia azedarach seed extract, 0.01-20 parts by weight of tea tree essential oil, and 0.01-8 parts by weight of eucalyptus oil.
6. The bactericide as claimed in claim 4, which comprises 0.1 to 5 parts by weight of extract of Andrographis paniculata Nees, 0.01 to 5 parts by weight of extract of Melia azedarach seeds, 0.01 to 15 parts by weight of tea tree essential oil, 0.01 to 5 parts by weight of eucalyptus oil, 800.1 to 30 parts by weight of Tween-800.1, 0.1 to 3 parts by weight of glycerin, and 5 to 30 parts by weight of ethanol.
7. The bactericide as claimed in claim 4, which comprises 0.1-5 parts by weight of Andrographis paniculata Nees extract, 0.01-5 parts by weight of Melia azedarach seed extract, 0.01-15 parts by weight of tea tree essential oil, 0.01-5 parts by weight of eucalyptus oil, tween-800.1-30 parts by weight of glycerin, 5-30 parts by weight of ethanol, and 20-60 parts by weight of water.
8. A method for producing a bactericide, characterized in that the method is used for producing a bactericide as claimed in any one of claims 1 to 7.
9. The method of claim 8, comprising the steps of:
(1) mixing tea tree essential oil and eucalyptus oil to obtain an essential oil mixture;
(2) mixing tween-80, ethanol and glycerol to prepare an auxiliary agent mixture, and then mixing the auxiliary agent mixture with the essential oil mixture prepared in the step (1) to prepare emulsified oil;
(3) and (3) adding the common andrographis herb extract, the chinaberry seed extract and water into the emulsified oil prepared in the step (2), and stirring and mixing to obtain the bactericide.
10. Use of a fungicide according to any one of claims 1 to 7 for the preparation of a bactericidal, antiviral or antifungal agent.
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