CN104920495A - Biopesticide for controlling cucumber bacterial leaf spot and preparation method of biopesticide - Google Patents
Biopesticide for controlling cucumber bacterial leaf spot and preparation method of biopesticide Download PDFInfo
- Publication number
- CN104920495A CN104920495A CN201510301811.XA CN201510301811A CN104920495A CN 104920495 A CN104920495 A CN 104920495A CN 201510301811 A CN201510301811 A CN 201510301811A CN 104920495 A CN104920495 A CN 104920495A
- Authority
- CN
- China
- Prior art keywords
- biopesticide
- grams
- parts
- bacterial leaf
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention discloses a biopesticide for controlling cucumber bacterial leaf spot and a preparation method of the biopesticide. The biopesticide is prepared from the following Chinese herbal medicine: galla chinensis, flos caryophyllata, herba asari, radix sophorae flavescentis, herba orostachyos, radix sanguisorbae and semen myristicae. Field tests show that the biopesticide 200-fold liquid and the biopesticide 300-fold liquid respectively have the control efficiencies of 94.6% and 87.2%, which are both remarkably higher than those of a 75% chlorothalonil wettable powder 600-fold liquid and 77% kocide wettable powder 400-fold liquid. Compared with the control, the two treatments are remarkable in prevention efficiency.
Description
Technical field
The present invention relates to field of biological pesticide, particularly relate to a kind of biopesticide and the preparation method that prevent and treat cucumber Bacterial Leaf Blight.
Background technology
Cucumber Bacterial Leaf Blight main harm blade, also cause harm young stem and petiole.When spire is caught an illness, symptom is not obvious, becoming leaf blade face to occur yellow district, there is water stain shape fleck in blade back, and Spot expansion is circular or subcircular, blade face, scab place is protruding, thinning, white, canescence, yellow or yellowish-brown, translucent in the middle of scab, scab border is not obvious, tool yellow halo, bacterial ooze is not obvious sometimes, sometimes at the dry bacterial ooze of vacuum side of blade adularescent.Children's stem is caught an illness, sick stem cracking.Fruit is caught an illness, and fruit is formed circular gray corrosion, wherein has yellow dry bacterial ooze.Cause of disease Xantpomonas campestris pv.cucubitae (Bryan) Dye, different name X.cucurottae(Bryan) Dowson claims campestris cucumber pvs oryzae and oryzicola (cucumber bacterial spot sick Xanthomonas campestris), belongs to bacterium.Thalline is shaft-like, and Dan Sheng, twin or chain life, have pod membrane.Bacterial growth thermophilic 25 ~ 30 DEG C, 36 DEG C can grow, and 49 DEG C lethal through 10 minutes.Propagated by seed-borne fungi, also can survive the winter in soil with invalid body, invade from seedling cotyledon or true leaf water hole and wound.After blade is caught an illness, germ, at propagated in cells, then enters vascular bundle, its spread in china.In protecting field, temperature is high, high humidity, and blade face condenses, leaf margin guttation, is beneficial to disease and occurs.Current prevention and controls mainly uses sufficient
base manure, timely draining after rain, ventilation and penetrating light between plant.While enhancing field management, reasonable selection
medicamentcontrol, spraying fungicide, their early stage use 50% tiger glue adipic acid copper (DT) wetting powder, 400 times of liquid or 77% to kill wetting powder 400 times of liquid.When occurring serious, can select simultaneously can kill mix with tiger glue adipic acid copper and spray.Also 80% spray gram wetting powder 600-800 times liquid can be selected according to the patient's condition and period of disease; 75% tpn wetting powder 600 times liquid; 50% carbendazol wettable powder, 800 times of liquid etc.Within every about 10 days, spray medicine once, 2-3 time altogether.As everyone knows, chemical pesticide Long-Time Service, easily make germ develop immunity to drugs, meanwhile, residual life, is long, contaminated environment, and harm humans is healthy.
Summary of the invention
The object of this invention is to provide a kind of control efficiency good, noresidue, non-environmental-pollution, the biopesticide of control cucumber Bacterial Leaf Blight be made up of pure Chinese herbal medicine.
The object of the invention is to be achieved through the following technical solutions, the biopesticide of this control cucumber leaf rot is made up of the Chinese medicine material of following weight portion: gall nut 15-21 part, cloves 15-21 part, root of Chinese wild ginger 15-21 part, kuh-seng 10-16 part, Orostachys fimbriatus 10-16 part, garden burnet 10-16 part, nutmeg 5-9 part.
Preferred: gall nut 18 parts, cloves 18 parts, the root of Chinese wild ginger 18 parts, kuh-seng 13 parts, Orostachys fimbriatus 13 parts, garden burnet 13 parts, nutmeg 7 parts.
Preparation method: produce by traditional ethanol extraction method.
The property of medicine of each Chinese medicine: cloves is containing volatile oil composition, and mainly containing eugenol in oil, accounting for 80% ~ 87%, is secondly β-cloves alkene, aceteugenol; Water or alcohol extract pair
pigroundworm has anesthesia and killing action; All inhibitory action is had to bacterium and pathogenic fungus; In vitro, cloves infected by influenza PR
8strain has inhibitory action.Gall nut is containing Gallotannin, and namely gallotannic acid reaches 50% ~ 78%, separately containing gallic acid, resin, wax, starch, fat etc.Gall nut contains tannic acid, has the effect of precipitating proteins, can solidify bacterioprotein.In vitro test shows: gall nut decoction all has significantly antibacterial and bactericidal action to staphylococcus aureus, beta hemolytic streptococcus, pneumococcus, Pseudomonas aeruginosa, shigella dysenteriae, bacillus anthracis, diphtheria bacillus, Escherichia coli, Typhoid and paratyphoid bacillus etc.The root of Chinese wild ginger is antibacterial, antivirus action kakuol preserved material, volatilization wet goods have certain In-vitro Inhibitory Effect to gram positive bacteria, hay bacillus and typhoid bacillus, and decoction also has inhibitory action to tubercle bacillus and typhoid bacillus.Kuh-seng, Orostachys fimbriatus, garden burnet, myristic extract all have inhibitory action to bacterium.
Field trial
Yinan County, Shandong Province is national high-quality cucumber production base, and cucumber Bacterial Leaf Blight is on the rise in vegetables old liberated area, the county, and the general underproduction 20 ~ 30%, serious subtracts more than 50%, even has no harvest.For proving the control efficiency of control cucumber Bacterial Leaf Blight biopesticide of the present invention, in county's modern agriculture Demonstration Garden cucumber in solar-greenhouse, carry out preventive effect experiment in March, 2014.
One, materials and methods
1, experimental group and control group is set up: experimental group reagent agent is biopesticide of the present invention, its preparation method is: take gall nut 180 grams, cloves 180 grams, the root of Chinese wild ginger 180 grams, kuh-seng 130 grams, Orostachys fimbriatus 130 grams, garden burnet 130 grams, nutmeg 70 grams, mix after respective pulverize, first cross 60 mesh sieves, rear mistake 100 mesh sieve, then put into the closed container of in-built 95% ethanol, put into shaken cultivation case 30 DEG C, after 24 h that vibrate under the condition of 150 r/min by it through 3 layers of filtered through gauze.Under 2500r/min condition, centrifugal 10min, discards sediment, under 45 degree, with Rotary Evaporators, supernatant is carried out reduced pressure concentration and causes lg/mL, obtain this biopesticide.
Control group reagent agent select 75% tpn wetting powder, 600 times of liquid, 77% to kill wetting powder 400 times of liquid spraying control.
2, test plant and disease: for examination cucumber variety " Tianjin spring No. 3 ", cucumber Bacterial Leaf Blight.
3, primary condition: soil is sandy loam, middle fertility, managerial skills are general, cucumber growth mid-term.
4, experimental scheme: be experimental field selected in Yinan County modern agriculture Demonstration Garden and plant in the greenhouse by solar heat of cucumber for years.200,300 times of liquid, two concentration are established in biopesticide field, are watered dose 30kg/ mu.Contrast medicament select 75% tpn wetting powder, 600 times of liquid, 77% to kill wetting powder 400 times of liquid, be watered dose 35kg/ mu.Blank is clear water, totally 5 process, repeats for 3 times, plot area 30m
2, randomized arrangement.March 18 carried out dispenser when cucumber middle and upper part blade starts to occur scab, used the super green 16 type sprayers of backpack, and quantitative and even is sprayed.
5, within 7 days, start to investigate after investigation content and method dispenser, take five point samplings, fixed point adventive, determine the way of the indefinite leaf of the number of sheets, often some investigation 30 blades (10, bottom, 10, middle parts, 10, top), the classification investigation state of an illness, the statistics incidence of disease and disease index, calculate control efficiency.Severity Scaling standard: 0 grade, without scab on leaf; 1 grade, lesion area accounts for whole leaf area less than 1/4; 2 grades, lesion area accounts for 1/4 ~ 1/2 of whole leaf area; 3 grades, lesion area accounts for 1/2 ~ 3/4 of whole leaf area; 4 grades, lesion area accounts for more than 3/4 of whole leaf area.
Disease index (%)=Σ (number of blade of sick level × this sick level) × 100/ the highest sick level × total leaf number.
Control efficiency (%)=(contrast disease index-process disease index) × 100/ contrast disease index.
Two, result of the test: biopesticide 200,300 times of liquid control efficiency are respectively 94.6%, 87.2%, all can kill to obtain the control efficiency of wetting powder 400 times of liquid apparently higher than 75% tpn wetting powder, 600 times of liquid and 77%, two process, compared with the control, preventive effect is remarkable.
Embodiment
Embodiment one: take gall nut 180 grams, cloves 180 grams, the root of Chinese wild ginger 180 grams, kuh-seng 130 grams, Orostachys fimbriatus 130 grams, garden burnet 130 grams, nutmeg 70 grams, same after respective pulverization, first cross 60 mesh sieves, rear mistake 100 mesh sieve, then put into the closed container of in-built 95% ethanol, put into shaken cultivation case 30 DEG C, after 24 h that vibrate under the condition of 150 r/min by it through 3 layers of filtered through gauze.Under 2500r/min condition, centrifugal 10min, discards sediment, under 45 degree, with Rotary Evaporators, supernatant is carried out reduced pressure concentration and causes lg/mL, obtain this biopesticide.
Embodiment two: take gall nut 160 grams, cloves 160 grams, the root of Chinese wild ginger 160 grams, kuh-seng 110 grams, Orostachys fimbriatus 110 grams, garden burnet 110 grams, nutmeg 60 grams.Preparation method is with embodiment one.
Embodiment three: take gall nut 200 grams, cloves 200 grams, the root of Chinese wild ginger 200 grams, kuh-seng 150 grams, Orostachys fimbriatus 150 grams, garden burnet 150 grams, nutmeg 80 grams.Preparation method is with embodiment one.
Claims (2)
1. prevent and treat a biopesticide for cucumber Bacterial Leaf Blight, it is characterized in that this biopesticide is made up of the Chinese medicine material of following weight portion: gall nut 15-21 part, cloves 15-21 part, root of Chinese wild ginger 15-21 part, kuh-seng 10-16 part, Orostachys fimbriatus 10-16 part, garden burnet 10-16 part, nutmeg 5-9 part.
2. a kind of biopesticide preventing and treating cucumber Bacterial Leaf Blight according to claim 1, is characterized in that this biopesticide is made up of the Chinese medicine material of following weight portion: gall nut 18 parts, cloves 18 parts, the root of Chinese wild ginger 18 parts, kuh-seng 13 parts, Orostachys fimbriatus 13 parts, garden burnet 13 parts, nutmeg 7 parts.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510301811.XA CN104920495A (en) | 2015-06-04 | 2015-06-04 | Biopesticide for controlling cucumber bacterial leaf spot and preparation method of biopesticide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510301811.XA CN104920495A (en) | 2015-06-04 | 2015-06-04 | Biopesticide for controlling cucumber bacterial leaf spot and preparation method of biopesticide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104920495A true CN104920495A (en) | 2015-09-23 |
Family
ID=54108209
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510301811.XA Pending CN104920495A (en) | 2015-06-04 | 2015-06-04 | Biopesticide for controlling cucumber bacterial leaf spot and preparation method of biopesticide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104920495A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105638330A (en) * | 2015-12-14 | 2016-06-08 | 高阳 | Method for preventing and controlling cucumber bacterial leaf blight on basis of biological experiment class |
| CN108029409A (en) * | 2017-12-19 | 2018-05-15 | 潘卫剑 | A kind of method for preventing cucumber leaf blight |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101940206A (en) * | 2010-09-30 | 2011-01-12 | 江苏天容集团股份有限公司 | Application of eugenol in preventing and controlling plant diseases and bactericide |
| CN101965848A (en) * | 2010-10-20 | 2011-02-09 | 中南林业科技大学 | Biogenic pesticide for efficiently preventing and controlling main diseases of Camellia oleifera and application thereof |
| CN104365704A (en) * | 2014-10-21 | 2015-02-25 | 刘峰 | Green agricultural compound Chinese herbal medicine bactericide |
-
2015
- 2015-06-04 CN CN201510301811.XA patent/CN104920495A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101940206A (en) * | 2010-09-30 | 2011-01-12 | 江苏天容集团股份有限公司 | Application of eugenol in preventing and controlling plant diseases and bactericide |
| CN101965848A (en) * | 2010-10-20 | 2011-02-09 | 中南林业科技大学 | Biogenic pesticide for efficiently preventing and controlling main diseases of Camellia oleifera and application thereof |
| CN104365704A (en) * | 2014-10-21 | 2015-02-25 | 刘峰 | Green agricultural compound Chinese herbal medicine bactericide |
Non-Patent Citations (2)
| Title |
|---|
| 余东坡: ""中草药醇提物抑菌活性筛选及其在青椒保鲜上的应用"", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 崔彦: ""防治黄瓜细菌角斑病的中草药提取物的筛选与研究"", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105638330A (en) * | 2015-12-14 | 2016-06-08 | 高阳 | Method for preventing and controlling cucumber bacterial leaf blight on basis of biological experiment class |
| CN108029409A (en) * | 2017-12-19 | 2018-05-15 | 潘卫剑 | A kind of method for preventing cucumber leaf blight |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Babu Joseph et al. | Bioefficacy of plant extracts to control Fusarium solani f. sp. melongenae incitant of brinjal wilt | |
| AU2016263055B2 (en) | Use of hydroxyapatite as a carrier of bioactive substances for treating vascular diseases in plants | |
| JP2020099332A (en) | Concentrated extract of algae, production method thereof and use of same in agriculture | |
| DE102007004500B4 (en) | Use of a formulation containing Sapindus extract for the treatment of a plant disease supported or caused by fungi and / or oomycetes | |
| CN105394111A (en) | Organic conditioner utilizing starch industrial production wastewater and preparation method thereof | |
| CN102217661B (en) | Botanical bactericide and preparation method and application thereof | |
| CN105532767A (en) | Insecticide, preparation method and application thereof | |
| CN105053064A (en) | Nuisanceless biological compound pesticide and preparation method thereof | |
| CN106478275A (en) | A kind of composite pesticide of preventing and treating tobacco diseases and preparation method thereof | |
| CN102036564A (en) | Environmentally friendly plant protection agents | |
| CN104255829A (en) | Polygonum aviculare botanical bactericide and preparation method thereof | |
| CN105685121A (en) | Botanical pesticide and preparation method thereof | |
| CN105613147A (en) | Method for controlling pests of greenhouse vegetables based on biological experiment of senior high schools | |
| CN104611154A (en) | Fruit and vegetable cleaning powder | |
| CN104920495A (en) | Biopesticide for controlling cucumber bacterial leaf spot and preparation method of biopesticide | |
| CN102204575B (en) | Silktree-albizia-extract-based preparation for controlling tetranychus cinnabarinus, and preparation and application thereof | |
| CN1299580C (en) | Plant source biological pesticide and producing method | |
| CN106508993A (en) | Pesticide resistant to tobacco mosaic virus and preparation method of pesticide | |
| CN116210728A (en) | Bactericide and preparation method and application thereof | |
| US11805784B2 (en) | Use of hydroxyapatite as a carrier of bioactive substances for treating vascular diseases in plants | |
| CN105685102A (en) | Environment-friendly sterilization insect repellant containing wood tar | |
| CN109287677A (en) | The purposes of flos caryophylli volatile oil | |
| CN102176831A (en) | A process for the production of a storage stable fungicidal extract of glycyrrhiza glabra for the control of phytopathogenic fungi and other plant diseases | |
| Kelaniyangoda et al. | Puccinia melampodii Diet. and Holow. as a biological control agent of Parthenium hysterophorus | |
| CN105542783A (en) | Humic acid type inorganic water-retaining agent by using edible vinegar factory waste liquid and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150923 |