CN114271261B - Efficient composite remains preservative and preparation method and preservation method thereof - Google Patents
Efficient composite remains preservative and preparation method and preservation method thereof Download PDFInfo
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Abstract
The invention discloses a high-efficiency composite remains preservative which mainly comprises the following substances in percentage by weight: 5-30% of glycerol, 10-15% of alcohol, 1-5% of formaldehyde, 5-10% of peroxyacetic acid, 1-2% of safflower oil, 2-4% of bay oil, 2-3% of glycerol acrylate, 1-4% of acrylic copolymer, 1-5% of lycopene, 1-3% of hyaluronic acid and the balance of deionized water. The remains preservative has the advantages of high-efficiency sterilization and disinfection, and can effectively improve putrefaction, hardening, discoloration and the like on the remains surface, so that the skin is relatively white, soft and moist. The fixed tissue structure is well preserved, has small volatility and is harmless to the body, and is better than 5 percent formalin and cadaver preserving fluid. Because the ionic liquid hardly volatilizes, is nontoxic and pollution-free, the ionic liquid can be recycled, the use cost is reduced, and the utilization rate is improved.
Description
Technical Field
The invention relates to the technical field of preservatives, and particularly relates to an efficient compound remains preservative and a preparation method and a preservation method thereof.
Background
After the tissue cells of animal or human remains lose vitality, the organelles of lysosome and the like are disintegrated, the tissues are decomposed under the action of various enzymes, each organ is softened and liquefied, and the remains are autolyzed. And because digestive juice in digestive organs of the remains generates autodigestion, and hypoxia, bacteria, toxins thereof and the like all accelerate the rupture of lysosomes. The massive release of hydrolytic enzymes in lysosomes destroys cells, disintegrates tissues, produces products which cannot be oxidized and increases the activity of the hydrolytic enzymes. Under the influence of putrefying bacteria, parasites and external environment inside and outside the remains, dead body tissues are accelerated to decompose, and the putrefaction of the remains is realized. In the process, a large amount of toxic malodorous gases such as amine, indole, hydrogen sulfide and the like are generated, and dead circulation is generated due to the expansion of hollow organs in the chest and abdominal cavity, so that a series of body putrefaction phenomena of severe change and vicious circulation such as odor, blackness, swelling, desquamation, deformation and the like occur.
The common remains preservation method is that formaldehyde is smeared, injected, soaked or frozen in a refrigerator for storage, and also a medicine solution is used as a preservative and mildew preventive, such as a 0.025-0.035% concentration molinate solution, or a preservative solution prepared by 0.05% PM agent and 10% ethanol, and the like, and often contains toxic substances such as benzene, phenol, aldehyde, heavy metal and the like, and the preservative measures have good effect on the remains preservation, but the remains treated in the way change the antenatal prototype, have pollution to the environment, and particularly cannot be repaired for the remains which are rotten. In addition, the formalin solution used alone has some disadvantages, and if the concentration of formaldehyde is insufficient, the bactericidal power is reduced, and the corpses become mildewed, which is mainly caused by the insufficient concentration of formaldehyde in the preservation solution. However, if the concentration of formaldehyde is too high, the odor is heavy, the permeability is not strong, and the damage to human is larger, so that the development of a novel efficient compound remains preservative is needed.
Disclosure of Invention
An efficient compound remains preservative mainly comprises the following substances in percentage by weight: 5-30% of glycerol, 10-15% of alcohol, 1-5% of formaldehyde, 5-10% of peroxyacetic acid, 1-2% of safflower oil, 2-4% of bay oil, 2-3% of glycerol acrylate, 1-4% of acrylic copolymer, 1-5% of lycopene, 1-3% of hyaluronic acid and the balance of deionized water.
In some embodiments, the high-efficiency compound remains preservative mainly comprises the following substances in percentage by weight: 25% of glycerol, 15% of alcohol, 2% of formaldehyde, 5% of peroxyacetic acid, 1% of safflower oil, 3% of bay oil, 2% of glycerol acrylate, 3% of acrylic copolymer, 3% of lycopene, 3% of hyaluronic acid and the balance of deionized water.
Of the above components, glycerin: colorless, odorless and sweet viscous liquid. The functional indications are as follows: can keep skin soft and elastic, is not damaged by dust, climate, etc., and has effect in preventing skin cold injury.
Alcohol: colorless, transparent and volatile liquid. The functional indications are as follows: and (5) sterilizing.
Formaldehyde: colorless and has a strong pungent odor. The functional indications are as follows: sterilizing, disinfecting and preserving agent.
Peroxyacetic acid: colorless or yellowish transparent liquid. The functional indications are as follows: can kill all microorganisms, and can quickly kill viruses, bacteria and fungi.
Safflower oil: reddish brown clear liquid, specific smell, pungent taste. The functional indications are as follows: the skin moistening oil can double care the skin of a human body, increase the expansibility and the cutability of oil on the skin, and increase the luster and the elasticity of the skin.
Bay oil extract: a bright yellow liquid with an aromatic odor. The functional indications are as follows: has good repairing and nourishing effects, and can make skin smooth and tender.
Glycerol acrylate: a water-soluble transparent gel. The functional indications are as follows: effectively lock the moisture in the skin, reduce the moisture loss and moisten the dry skin.
Acrylic acid copolymer: a material having extremely high adhesiveness. The functional indications are as follows: used for antistatic and filming agent.
Lycopene: natural red open chain hydrocarbon carotene, deep red crystal. The functional indications are as follows: has radioprotective, skin caring and skin caring effects.
Hyaluronic acid: a gel-like substance. The functional indications are as follows: effectively supplements water and achieves the effect of locking water. Can improve skin physiological condition, enhance skin nutrient supply, prevent skin oxidation, and smooth wrinkle.
Deionized water: colorless, odorless, and odorless. The functional indications are as follows: has moisture keeping effect, and can completely resist xerosis cutis caused by environmental and temperature changes.
In some embodiments, the high-efficiency compound remains preservative can be prepared by weighing each component of the high-efficiency compound remains preservative, and uniformly stirring and mixing the components.
In some embodiments, the method for preserving remains by using the high-efficiency compound remains preservative comprises the following steps:
s1: washing the surface of the remains with water, emptying the liquid in the body groove, wiping the remains with 5% formalin solution, and drying with an electric fan.
S2: the remains are wiped by the high-efficiency compound remains preservative, and the common carotid artery of the remains is perfused with the high-efficiency compound remains preservative.
S3: ligating the proximal end and the distal end of a common carotid artery incision after the perfusion is finished, sewing an operation incision, and immersing the remains in the efficient compound remains preservative after 24 hours.
In some embodiments, in the step S2, the perfusion amount of the high-efficiency compound remains preservative is 30% -40% of the body weight of the remains.
In some embodiments, in step S2, the electric fan drying time is 7 days, and 2 hours are blown in the morning and 2 hours are blown in the afternoon each day.
Compared with the prior art, the remains preservative has the beneficial effects that the remains preservative has high-efficiency sterilization and disinfection, can effectively improve putrefaction, hardening, discoloration and the like on the remains surface, and enables the skin to be relatively white, soft and moist. Compared with the corpse preservation solution and 5 percent formalin Lin Yelai, the corpse preservation solution not only achieves the long-term fixed preservation effect of formalin, but also realizes the antisepsis, the mildew prevention and the color preservation and the moisture preservation of the corpse preservation solution in short-term preservation, and the fixed tissue structure is well preserved, has small volatility and is harmless to the body, and is better than the 5 percent formalin and the corpse preservation solution. Because the remains preservative has almost no volatilization, no toxicity and no pollution, the remains preservative can be recycled, the use cost is reduced and the utilization rate is improved.
Drawings
FIG. 1 shows the observation results of tissue section and optical microscope after storing three different specimen storage solutions, wherein A is normal fresh muscle tissue, B is the high-efficiency compound remains preservative of example 1, C is a specimen of a corpse preservation solution, and D is a 5% formalin specimen.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1: preparation of high-efficiency composite remains preservative
Taking the following substances in percentage by weight: 25% of glycerol, 15% of alcohol, 2% of formaldehyde, 5% of peroxyacetic acid, 1% of safflower oil, 3% of bay oil, 2% of glycerol acrylate, 3% of acrylic copolymer, 3% of lycopene, 3% of hyaluronic acid and the balance of deionized water, and stirring and mixing uniformly to obtain the efficient composite remains preservative.
Example 2: the efficient composite remains preservative has different proportions, killing effect on bacteria and fungi and effect on remains
Preparing a remains preservative according to the following proportion, and testing the effect:
table 1: remains antistaling agent with different proportions
The test strains are escherichia coli, staphylococcus aureus, bacillus megaterium, pseudomonas fluorescens, aspergillus niger, aspergillus flavus, chromotropic mold, penicillium citrinum and paecilomyces variotii, the detection conditions are that the bacteria are cultured at 36 ℃, the mold is cultured to logarithmic growth phase at 28 ℃, the antibacterial activity experiments are compared, and the size of the antibacterial zone is tested. Meanwhile, the remains are observed to be treated by the preservatives with different proportions, and the treatment method comprises the following steps of S1: washing the surface of the remains with water, emptying the liquid in the body groove, wiping the remains with 5% formalin solution, and drying with an electric fan. The electric fan is blown for 7 days, 2 hours in the morning and 2 hours in the afternoon every day. S2: the remains are wiped by the high-efficiency compound remains preservative, and the high-efficiency compound remains preservative is filled into the common carotid artery of the remains, wherein the filling amount is 30-40% of the weight of the remains. . S3: ligating the proximal end and the distal end of a common carotid artery incision after the perfusion is finished, sewing an operation incision, and immersing the remains in the efficient compound remains preservative after 24 hours. The change in the remains was observed on day 10. The results are as follows:
table 2: the effects of the remains fresh-keeping agents with different proportions on bacteria, mould and remains treatment are compared
As can be seen from the table above, after the remains fresh-keeping agent is used for treatment, the propagation of bacteria and mould is effectively inhibited, and after the remains are treated by the remains fresh-keeping agent, the remains are moist, glossy, white, soft and not easy to rot or harden.
Example 3: influence of different-component specimen storage liquid on specimen storage quality
Example 1 the efficient composite remains preservative
Comparative example 1 Formaldehyde-free cadaver preservative solution, wenzhou, zhejiang, production area, main ingredients of mildew-proof preservative, ethanol, glycerol, 50% glutaraldehyde and the like
Comparative example 2 was 5% formalin, hangzhou, origin.
The experimental method comprises the following steps: 18 pieces of glass jars (with the same size and provided with movable glass covers) filled with knee joint specimens (waste corpse small-sized local knee joint specimens for source teaching, pretreated for 1 year by using 5% formalin liquid) are placed in 18 lattices with the same height and size in a specimen display cabinet in the same laboratory. Tissue sections and optical microscopic observation of the material are required. A vernier caliper (the accuracy is 0.02 mm) and an IQ-250 type formaldehyde detector.
The 18 cells of the anatomical laboratory display case are independent of each other and are provided with a movable glass door. The amount of liquid in each jar was varied and 1 knee joint specimen was placed, completely submerged in the liquid and capped. There are 6 glass jars for each preservation solution. The cover opening inspection is carried out in the same period of the continuous year (the cover opening time is 2019-31 days of 8 months every year 2021), liquid is not added in the process, and the volatilization condition of the liquid in 18 grids is measured. The cadaver specimen was visually inspected for mildew and fluid color, and the procedure lasted for two years. And detecting and analyzing the formaldehyde content of 5 points in the range of 5cm around the glass cylinder by using an IQ-250 type formaldehyde detector. Taking the tissue of the same part of all specimens for section observation and comparison.
Observation of first storage effect in 2019 on day 8, month 31, the liquid level of the three liquids in the laboratory showcase was observed for the first time, and in example 1, comparative example 1 and comparative example 2, the liquid level was decreased by 0.5cm,3.8cm and 2.3cm, respectively. The color of the liquid and the mildew condition of the specimen are clear and transparent in the embodiment 1, and the specimen is not exposed and does not mildew; the cadaver preservation solution is turbid and milky compared with the cadaver preservation solution in the comparative example 1, and 1 mildew spot with the diameter of 0.2cm is seen on an exposed part; 5% formalin, comparative example 2, stored the liquid with turbid color and no mildew stains on the exposed portions.
The second storage effect was observed to be a drop of 1.2cm,7.8cm,7.2cm in the liquid level of the three liquids in the laboratory, example 1, comparative example 1 and comparative example 2, at 31/8/2020, second decap check. The color of the liquid and the mildew condition of the specimen are clear and transparent in the embodiment 1, and the specimen is not exposed and does not mildew; cadaver preservative fluid, comparative example 1 is turbid, milky white precipitate, and 4 mildew spots with the diameter of 0.3cm are seen on the naked part; the 5% formalin preservation solution of comparative example 2 was turbid and yellowish in color, and 2 mildew spots with a diameter of 0.1cm were observed on the exposed part. The long-term storage effect of example 1 is significantly better than that of the other two liquids.
The third storage effect observation is carried out at 8 months and 31 days in 2021, the liquid level of the three liquids in the laboratory is respectively reduced by 1.7cm,14.5cm and 13.7cm after the third cover opening inspection. The color of the liquid and the mildew condition of the specimen are clear and transparent in the embodiment 1, and the specimen is not exposed and does not mildew; the cadaver preservation solution is turbid and dark red compared with the cadaver preservation solution in the comparative example 1, and the liquid surface of the exposed part is fused and covered by large mildew stains; the 5% formalin preservation solution of comparative example 2 was cloudy and dark yellow in color, and 2 mildew spots with a diameter of 0.5cm were observed on the exposed portions.
The measured results of the IQ-250 formaldehyde detector show that the formaldehyde content values of three preservation solutions at 5 points in the range of 5cm around the glass cylinder in the grid in the exhibition cabinet are shown in Table 3.
Table 3: comparison of Formaldehyde content between the remains preservative in example 1 and the conventional remains treatment reagent
Comparison with example 1. P < 0.01
The above tests show that example 1 is a novel long-term mildew-proof, antiseptic preservative solution. Almost no vapor pressure at room temperature, excellent chemical and thermodynamic stability, and suitability for wide temperature range, compared with the traditional industrial organic solvent, the organic solvent is also called as 'green solvent' because the vapor pressure can not be measured, and the organic solvent is non-volatile and pollution-free.
Example 4: fixation of specimen tissue
When the storage effect is observed in 2021, 8, 31 days, tissue sections and optical microscopic observation materials are obtained, wherein A is normal fresh muscle tissue, B is the high-efficiency compound remains preservative in example 1, C is a specimen of a corpse preservation solution, and D is a 5% formalin specimen.
As can be seen from the section, the fixing effect of the specimen preserved by the efficient compound remains preservative in the embodiment 1B and the specimen preserved by the D5% formalin Lin Baocun is better than that of the specimen preserved by the C corpse preserving fluid, and the specimen preserved by the efficient compound remains preservative is obviously better than that of the specimen preserved by the 5% formalin fluid and the corpse preserving fluid due to the structure under the tissue section mirror.
Example 5: sensory analysis was performed by observing the hand feeling, color quality, fixing effect, and mildew phenomenon of the specimen for the first uncapping for 2019 and the second uncapping for 2020 and 31 months, and the change results are shown in the following tables 4 and 5:
table 4 first uncap 2019 year 8 month 31 day note book effect observation
TABLE 5 Observation of effect of standard book of 31/8/month/2020 by opening cover for the second time
As can be seen from the analysis of the above table, the specimen slice tissues of the first two preservation solutions of the three preservation solutions are loose, and the others are unchanged. For remains, the remains need to be stored for a long time and cannot mildew, and the liquid for soaking the specimens cannot volatilize quickly, so the high-efficiency compound remains preservative comprehensively compared with the high-efficiency compound remains preservative in the embodiment 1 has the advantages of high-efficiency sterilization and disinfection, and can effectively improve the decay, hardening, color change and the like of the remains surface, and ensure that the skin is relatively white, soft and moist.
While the foregoing is directed to the preferred embodiment of the present invention, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention.
Claims (6)
1. The compound remains preservative is characterized by mainly comprising the following substances in percentage by weight: 5-30% of glycerol, 10-15% of alcohol, 1-5% of formaldehyde, 5-10% of peroxyacetic acid, 1-2% of safflower oil, 2-4% of bay oil, 2-3% of glycerol acrylate, 1-4% of acrylic copolymer, 1-5% of lycopene, 1-3% of hyaluronic acid and the balance of deionized water.
2. The composite remains preservative according to claim 1, which is characterized by mainly comprising the following substances in percentage by weight: 25% of glycerol, 15% of alcohol, 2% of formaldehyde, 5% of peroxyacetic acid, 1% of safflower oil, 3% of bay oil, 2% of glycerol acrylate, 3% of acrylic copolymer, 3% of lycopene, 3% of hyaluronic acid and the balance of deionized water.
3. The preparation method of the composite remains preservative is characterized by comprising the following steps: the compound remains preservative according to the claim 1 or 2, and the components are weighed and stirred and mixed uniformly to prepare the compound remains preservative.
4. A method of preserving, comprising the steps of:
s1: washing the surface of the remains with water, emptying the liquid in the body groove, wiping the remains with 5% formalin solution, and drying with an electric fan;
s2: wiping a remains with the compound remains preservative of claim 3, and perfusing the compound remains preservative into the common carotid artery of the remains;
s3: ligating the proximal end and the distal end of a common carotid artery incision after the perfusion is finished, suturing the surgical incision, and immersing the remains in the compound remains preservative according to claim 3 after 24 hours.
5. The preservation method according to claim 4, wherein in the step S2, the compound remains preservative is infused with an amount of 30% -40% of the body weight of the remains.
6. The preservation method according to claim 4, wherein in the step S2, the electric fan drying time is 7 days, and the electric fan is blown for 2 hours in the morning and 2 hours in the afternoon every day.
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| CN1732775A (en) * | 2004-08-10 | 2006-02-15 | 任祖翰 | Composition for short-term remains preservation, its preparation process and application |
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