CN101036455A - Preservative and its preparation method - Google Patents
Preservative and its preparation method Download PDFInfo
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- CN101036455A CN101036455A CN 200710034262 CN200710034262A CN101036455A CN 101036455 A CN101036455 A CN 101036455A CN 200710034262 CN200710034262 CN 200710034262 CN 200710034262 A CN200710034262 A CN 200710034262A CN 101036455 A CN101036455 A CN 101036455A
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Abstract
The invention discloses an antiseptic agent formula for use in an animal or human corpse and sample and its preparation and using method, which relates to the biological and medical aseptic technic field. The antiseptic agent without penetrating odor and with better mould proof effect is composed of 1% to 10% of main metal salt by weight, 0.3% to 2.5% organic acid by weight, 1% to 2.5% crosslinking agent by weight, 2% to 5% accessory metal salt by weight, and 80% to 95% water by weight, which can be used for antisepsis fixation and antisepsis preservation for human or animal corpses.
Description
Technical field
The present invention relates to biological and medical science aseptic technic field, specifically is about the preservative prescription of a kind of animal or human's of being used for class corpse, sample and preparation thereof, using method.
Background technology
After animal or human's death, if tissue automatic soup-dissolving and corruption just can take place without preservative treatment in corpse soon, and it shows as each organ and occurs softening or liquefaction, structural collapse, clasmatosis.Produce the toxic products that various kinds of amine, indoles, methyl indol, hydrogen sulphide etc. have strong odor simultaneously.Corrupt basic reason takes place corpse is the decomposition of enzyme.These have powerful bioactive enzyme and mainly contain two sources: the one, be present in the lysosome of body self cell; The 2nd, by the bacterium generation of continuous breeding.Because the chemical nature of enzyme is a protein, so all various physical chemical factors of protein denaturation inactivation that can make all have the different antisepsis of degree.
Physics anti-corrosion method commonly used has heating, freezing, ray or ultraviolet irradiation, drying and dehydrating etc.But the anticorrosion many disadvantages that exists of physical method as cost height, condition harshness, easily cause metaplasia etc., is used less in medical science and biological study.
The common chemical preservative has aldehydes, phenols, alcohols, heavy metal salt and some organic acid.Now, the chemical preservative that generally uses of people is a formalin.The formalin of the 35%-40% that sells on the market is commonly called as formalin.Use formaldehyde and antiseptic to have many advantages, but following major defect also arranged: the one, tissue after formalin is handled, hard and embrittlement, toughness and elasticity are little, easily rupture because of the tractive in using; The 2nd, anti-mold effect is poor, and sample or corpse are deposited under the situation that there was dampness in the air, will go mouldy soon; The 3rd, give out strong asphyxiating penetrating odor, eye, respiratory mucosa there are the strong impulse effect, cause serious air pollution; The 4th, the toxicity of formaldehyde is big, and it shows three broad aspect such as spread effect, organ injury and carcinogenic short cancer effect to the harm of human body.In order to reduce the harm of formaldehyde to human body, people have carried out unremitting effort and exploration aspect the corpus antiseptic improvement, made 0.05% PM agent and added 10% alcoholic solution, the replacement formalins such as mould enemy's solution of the phenol solution of 2%-3%, 0.025%-0.035% concentration are used for corpse and soak and preserve.But these solution are poor to the anticorrosion fixed effect of corpse, can only be used for the immersion of the corpse of the anticorrosion fixedly processing of process and preserve, and can not be used for the anticorrosion fixing of corpse.
Summary of the invention
Technical problem underlying solved by the invention provides a kind of having no irritating odor and has the preservative of strong anti-mildew effect simultaneously, can be used for the anticorrosion fixing and anticorrosion preservation of human or animal's corpse.
Preservative disclosed in this invention is that the main slaine of 1%-10%, the organic acid of 0.3%-2.5%, the crosslinking agent of 1%-2.5%, the auxilliary slaine of 2%-5% and the water of 80%-95% are formed by percentage by weight.
Main slaine of the present invention is meant molysite, zinc salt, aluminium salt.Described molysite is meant ferric trichloride, ferric sulphate, and zinc salt is meant zinc sulphate, zinc chloride, and aluminium salt is meant aluminum sulfate, aluminium chloride.
Organic acid of the present invention is meant citric acid, succinic acid, lactic acid.
Crosslinking agent of the present invention is a methenamine.
Auxilliary slaine of the present invention is meant sodium salt, sylvite, calcium salt.Described sodium salt is meant sodium chloride; Sylvite is meant potassium chloride, potassium nitrate; Calcium salt is meant calcium chloride, nitrate of lime.
Second technical problem solved by the invention provides the compound method of aforementioned preservative.
The compound method of preservative of the present invention is as follows:
By aforementioned formula take by weighing main slaine, organic acid adds in the entry, is mixed with A liquid after the dissolving.By prescription take by weighing crosslinking agent, auxilliary slaine adds in the entry, is mixed with B liquid after the dissolving.A liquid and B liquid are mixed, stir promptly.
The 3rd technical problem to be solved by this invention is the range of application that discloses aforementioned preservative.
Anticorrosion fixative of the present invention can be used for the anticorrosion fixing of human or animal's corpse and the corpse that is used for having handled through anticorrosion fixative, the immersion preservation or the spray of animal or human's body dissec are preserved moisture.
Preservative prescription disclosed by the invention is scientific and reasonable, does not contain volatile component, makes the preservative odorlessness of preparation.Listed main slaine all has brute force and protein coagulating, precipitation and make its sex change inactivation fast in the prescription, and the decomposition of having blocked cathepsin has suppressed the growth and breeding of bacterium, thereby prevents corrupt the generation.Organic acid can quicken the dissolving of slaine and prevent to produce precipitation because of the flocculation of main slaine, has increased the stability of solution.The adding methenamine has the effect of three aspects, and the one, disinfection, the 2nd, neutralization is acid, and the 3rd, with proteins react, collaborative main slaine solidifies protein, to reduce its consumption.Avoid causing being fixed the tissue contracts distortion because of the consumption of main slaine is excessive.
The listed salt of preservative of the present invention does not comprise hypertoxic slaines such as mercury salt, lead salt, and some is used as flavor enhancement, preservative or nourishing additive agent selected composition in food industry, and some composition medically is being used as nutritional supplement or disease medicine.These compositions are little to the toxicity of human body, effectively controlled long-term use or contactee's poisoning risk, belong to low toxic and environment-friendly type preservative.Preservative of the present invention does not contain volatile ingredient, has reduced possibility lost because of volatilizing, that the concentration reduction was lost efficacy, has guaranteed that preservative is permanently effective.The main slaine of preservative of the present invention is the main component that tissue solidifies, and adjusts its concentration the hardness of organizing that is fixed is changed, and can adapt to the needs of making the different hardness sample better.
One, the fixation of tissue measure of merit of preservative of the present invention.
Test one:
1, prescription
Ferric trichloride 6g citric acid 2.3g methenamine 1.8g sodium chloride 4.5g water 136ml
2, sample
Each one in lean pork, fat meat, liver, kidney and brain are 1.5*2*2 (cm
3) size.
3, sample is handled
The tested tissue piece is put into above-mentioned impregnating fluid soaked for two weeks.
4, testing result
1. naked eyes are seen: impregnating fluid is faint yellow, transparent, odorlessness.The not variable color of fat meat piece, liver, kidney, lean meat piece keep primary colors, color thin out.That the brain piece is is faint yellow, the protein boundary is clear.Solidifying all appears in each piece of tissue, hardness increases, and wherein lean meat, kidney tissue block are obvious.
2. microscopic examination: liver, kidney, lean meat, brain are made histologic section, be placed on microscopically and observe after HE dyeing, see that institutional framework is clear, complete, cell nucleus, cytoplasm are fixed well, form is normal.
Test two:
Get virgin corpse 12 tools of 1-3 year, wherein male corpse 8 tools, women corpse 4 tools.Be divided into each 6 tool of experimental group and control group at random.Experimental group is carried out anticorrosion fixing and preservation by prescription of the present invention, and control group carries out anticorrosion fixing and preservation with 10% formalin.All preserved at normal temperatures 1 year for two groups.Its result is as follows:
Experimental group: preserve liquid and be faint yellow, limpid transparent, odorlessness does not have fungus growth on liquid level and the chamber wall, and pH value is 3.0.Corpse does not have corruption, the skin pinkiness.The elasticity of muscle is good, and the joint is movable.Anatomic observation is seen each internal organs preservation original position, and hardening is in various degree all arranged.Liver,kidney,spleen is brown, and stomach, large intestine, small intestine are creamy white.Peritoneal exudate odorlessness, pH value are 6.0.
Control group: preserve liquid and be water white transparency, the strong impulse smell is arranged, several mildews are arranged on the chamber wall, the liquid pH value is 6.0.Corpse does not have corruption, and skin is crineous.The elasticity of muscle is poor, and the joint is stiff substantially.Anatomic observation is seen each internal organs preservation original position, obviously sclerosis, and color is pale.Peritoneal exudate has penetrating odor, and pH value is 6.5.
Two, the fungistatic effect of preservative of the present invention test
1, bacterial classification is surveyed in choosing: staphylococcus aureus, Escherichia coli, proteus, surperficial staphylococcus, streptococcus, hay bacillus.
2, detection method: dull and stereotyped inhibition zone method (using nutrient agar panel)
3, detected temperatures: 35 ℃ ± 1 ℃
4, detection time: 24 hours
5, testing result: use kind of calliper.Preservative of the present invention all has the obvious suppression effect to selected bacterial classification.The antibacterial circle diameter reckling is 16mm, and the maximum diameter is 25mm.
Three, the anti-mildew of preservative of the present invention becomes effect detection
1, bacterial classification is surveyed in choosing:
Penicillium citrinum, Mucor racemosus, blue colter Mucor, aspergillus flavus, variable color aspergillus, Trichoderma viride, grayish green colter are mould, mucor mucedo, cladosporium herbarum.
2, detection method: dull and stereotyped inhibition zone method.
3, detected temperatures: 26 ℃ ± 2 ℃
4, incubation time: 72 hours
5, testing result:
Preservative of the present invention all has inhibitory action to tested mould, and antibacterial circle diameter is as follows: Penicillium citrinum 45mm, Mucor racemosus 42mm, blue colter Mucor 43mm, aspergillus flavus 40mm, variable color aspergillus 38mm, Trichoderma viride 45mm, the mould 29mm of grayish green colter, mucor mucedo 26mm, cladosporium herbarum 23mm.
In sum, the present invention is that a kind of having no irritating odor has the preservative of stronger antibacterial anti-mildew effect simultaneously, can be used for the anticorrosion fixing and anticorrosion preservation of human or animal's corpse.
Specific embodiment
Embodiment one:
Ferric trichloride 50g citric acid 15g methenamine 12g sodium chloride 30g water 893ml.
By the prescription proportioning water that ferric trichloride and citric acid add 435ml is put into nonmetal canister, mix, stir, make A liquid after the dissolving.The water that methenamine and sodium chloride is added 458ml is put into nonmetallic vessel again, mixes and stirs, and makes B liquid after the dissolving, again will A liquid and B liquid be obtain preservative after mixing stirring and dissolving.During preservation, must be with A, B liquid respectively with holding in the nonmetallic vessel.
Embodiment two:
Zinc chloride 50g citric acid 15g methenamine 12g potassium chloride 30g water 893ml.
By the prescription proportioning water that zinc chloride and citric acid add 435ml is put into nonmetal canister, mix, stir, make A liquid after the dissolving.The water that methenamine and potassium chloride is added 458ml is put into nonmetallic vessel again, mixes and stirs, and makes B liquid after the dissolving, again will A liquid and B liquid be obtain preservative after mixing stirring and dissolving.Store method is with embodiment one.
Implement three:
Aluminium chloride 50g citric acid 15g methenamine 12g nitrate of lime 30g water 893ml.
By the prescription proportioning water that aluminium chloride and citric acid add 435ml is put into nonmetal canister, mix, stir, make A liquid after the dissolving.The water that methenamine and nitrate of lime is added 458ml is put into nonmetallic vessel again, mixes and stirs, and makes B liquid after the dissolving, again will A liquid and B liquid be obtain preservative after mixing stirring and dissolving.Store method is with embodiment one.
Implement four:
Aluminum sulfate 50g citric acid 15g methenamine 12g potassium nitrate 30g water 893ml.
By the prescription proportioning water that aluminum sulfate and citric acid add 435ml is put into nonmetal canister, mix, stir, make A liquid after the dissolving.The water that methenamine and potassium nitrate is added 458ml is put into nonmetallic vessel again, mixes and stirs, and makes B liquid after the dissolving, again will A liquid and B liquid be obtain preservative after mixing stirring and dissolving.Store method is with embodiment one.
Claims (9)
1, a kind of preservative, its component and content range are as follows:
The water of the crosslinking agent of the main slaine of 1%-10%, the organic acid of 0.3%-2.5%, 1%-2.5%, the auxilliary slaine of 2%-5% and 80%-95% is formed.
2, preservative as claimed in claim 1 is characterized in that: described main slaine is meant molysite, zinc salt, aluminium salt.
3, preservative as claimed in claim 2 is characterized in that: described molysite is meant ferric trichloride, ferric sulphate, and zinc salt is meant zinc sulphate, zinc chloride, and aluminium salt is meant aluminum sulfate, aluminium chloride.
4, preservative as claimed in claim 1 is characterized in that: described organic acid is meant citric acid, succinic acid, lactic acid.
5 preservatives as claimed in claim 1 is characterized in that: described crosslinking agent is a methenamine.
6, preservative as claimed in claim 1 is characterized in that: described auxilliary slaine is sodium salt, sylvite, calcium salt.
7, preservative as claimed in claim 6 is characterized in that: described sodium salt is meant sodium chloride; Sylvite is meant potassium chloride, potassium nitrate; Calcium salt is meant calcium chloride, nitrate of lime.
8, a kind of compound method of preservative is as follows:
By aforementioned formula take by weighing main slaine, organic acid adds in the entry, is mixed with A liquid after the dissolving.By prescription take by weighing crosslinking agent, auxilliary slaine adds in the entry, is mixed with B liquid after the dissolving.A liquid and B liquid are mixed, stir promptly.
9, a kind of range of application of preservative is: the corpse that can be used for the anticorrosion fixing of human or animal's corpse and be used for having handled through anticorrosion fixative, the immersion of animal or human's body dissec are preserved or are sprayed and preserve moisture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200710034262XA CN100534302C (en) | 2007-01-10 | 2007-01-10 | Preservative and its preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200710034262XA CN100534302C (en) | 2007-01-10 | 2007-01-10 | Preservative and its preparation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101036455A true CN101036455A (en) | 2007-09-19 |
| CN100534302C CN100534302C (en) | 2009-09-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB200710034262XA Expired - Fee Related CN100534302C (en) | 2007-01-10 | 2007-01-10 | Preservative and its preparation method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102175496A (en) * | 2011-01-21 | 2011-09-07 | 哈尔滨格林标本技术开发有限公司 | Formaldehyde-free fixative for tissue sample |
| CN101438698B (en) * | 2008-12-17 | 2011-11-30 | 中南大学 | Anti-corrosive perfusate and preservative fluid of biological wet carcase |
| CN103988834A (en) * | 2014-05-18 | 2014-08-20 | 南阳医学高等专科学校 | Animal specimen preserving fluid |
| CN104521941A (en) * | 2014-12-13 | 2015-04-22 | 李洋 | Antiseptic powder for preparing animal specimens and application thereof |
| CN105052891A (en) * | 2015-07-16 | 2015-11-18 | 内蒙古科技大学包头医学院 | Long-stem storage method for anatomical visceral specimen |
-
2007
- 2007-01-10 CN CNB200710034262XA patent/CN100534302C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101438698B (en) * | 2008-12-17 | 2011-11-30 | 中南大学 | Anti-corrosive perfusate and preservative fluid of biological wet carcase |
| CN102175496A (en) * | 2011-01-21 | 2011-09-07 | 哈尔滨格林标本技术开发有限公司 | Formaldehyde-free fixative for tissue sample |
| CN102175496B (en) * | 2011-01-21 | 2013-04-03 | 哈尔滨格林标本技术开发有限公司 | Formaldehyde-free fixative for tissue sample |
| CN103988834A (en) * | 2014-05-18 | 2014-08-20 | 南阳医学高等专科学校 | Animal specimen preserving fluid |
| CN104521941A (en) * | 2014-12-13 | 2015-04-22 | 李洋 | Antiseptic powder for preparing animal specimens and application thereof |
| CN104521941B (en) * | 2014-12-13 | 2016-08-03 | 华北理工大学 | A kind of antiseptin making zoological specimens and application |
| CN105052891A (en) * | 2015-07-16 | 2015-11-18 | 内蒙古科技大学包头医学院 | Long-stem storage method for anatomical visceral specimen |
| CN105052891B (en) * | 2015-07-16 | 2017-03-08 | 内蒙古科技大学包头医学院 | The long-term preservation method of anatomy internal organ sample |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100534302C (en) | 2009-09-02 |
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Granted publication date: 20090902 Termination date: 20110110 |