CN110604126A - Non-formaldehyde environment-friendly pathological tissue fixing agent - Google Patents
Non-formaldehyde environment-friendly pathological tissue fixing agent Download PDFInfo
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- CN110604126A CN110604126A CN201810616429.1A CN201810616429A CN110604126A CN 110604126 A CN110604126 A CN 110604126A CN 201810616429 A CN201810616429 A CN 201810616429A CN 110604126 A CN110604126 A CN 110604126A
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- buffer solution
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- pathological tissue
- fixing agent
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
Abstract
The invention discloses a non-formaldehyde environment-friendly pathological tissue fixing agent, which relates to the technical field of pathological tissue fixing and is characterized by comprising plant tannin and a buffer solution, wherein the content of tannin in the plant tannin is more than 8%, the buffer solution is a weak acid solution, and the buffer solution is a mixed solution of sodium hydroxide, citric acid, urea, phosphoric acid and deionized water, wherein the mass fraction of the plant tannin is 2-10%, the mass fraction of the sodium hydroxide is 0.5-1%, the mass fraction of the citric acid is 0.865-3.5%, the mass fraction of the urea is 0.045-0.2%, the volume fraction of the phosphoric acid is 0.2-0.48%, and the balance is deionized water. The invention can completely replace formalin solution, and simultaneously solves the problems of pungent smell, volatile active ingredients, poor softness of preserved pathological tissues, poor tissue fixing effect and the like of the traditional fixative.
Description
Technical Field
The invention relates to the technical field of pathological tissue fixation, in particular to a non-formaldehyde environment-friendly pathological tissue fixing agent.
Background
Formalin is used most widely as a cadaver preservative, formaldehyde and benzene are determined as carcinogens by world health organization, formaldehyde is a recognized carcinogen in the world, is listed as a high-toxicity chemical, can strongly stimulate eyes, skin, respiratory mucosa and the like, finally causes immunologic dysfunction, liver injury and influence on a nerve central system, and can cause diseases such as fetal deformity, leukemia, chronic respiratory diseases, nasopharyngeal carcinoma, female menstrual disorder, acute dysthymia and the like. In addition, the specimen preserved for a long time by the formaldehyde solution can be hardened and yellowed, and simultaneously has white precipitate and turbidity, and the formaldehyde solution can also be reduced in concentration due to the volatilization of the formaldehyde, so that the function of preserving the specimen is gradually lost.
While the harm of the formaldehyde solution for preserving specimens is gradually recognized, some substitute products for the formaldehyde solution, such as benzoic acid-ethanol-potassium nitrate solution, concentrated seawater saturated salt solution, surfactant solution, molinate solution, etc., are also appearing in succession.
The study of the preservation of specimens with benzoic acid-ethanol-potassium nitrate solution began earlier. The benzoic acid has the main function of killing fungi and playing a role in resisting mildew; ethanol can sterilize and dehydrate; the potassium nitrate can improve the permeability, enhance the corrosion resistance and the mildew resistance and has the color retention effect on specimens; in addition, in some damp and hot areas, a small amount of formaldehyde solution is additionally added for achieving better anticorrosion and fixation effects. The multi-component chemicals are matched for use, so that the specimen can be well preserved. The specimen preserved by the fixing agent has natural color and luster, soft texture, strong hygroscopicity and no mildew phenomenon, the fixing agent is always clear and transparent, has no precipitate, has no obvious difference with the specimen preserved by the traditional formaldehyde solution, and is an ideal specimen fixing agent in a short period. However, the fixative also has a number of disadvantages. Because of the volatility of ethanol, the fixative gradually loses effectiveness, and is difficult to popularize especially in hot regions. In addition, the solubility of benzoic acid is low, and the solubility of benzoic acid in 100g of water at 18 ℃ is only 0.27g, so that the mildew-proof effect cannot be achieved by increasing the dosage of benzoic acid. In addition, if the specimen fixative is not used together with a small amount of formaldehyde, the fixation effect of the specimen fixative cannot meet the requirement of specimen preservation, and the specimen preserved for a long time can generate tissue adhesion. Therefore, in the future research, the formulation components of the benzoic acid-ethanol-potassium nitrate solution need to be improved to achieve the ideal effect.
Due to the lack of effective environment-friendly preservative solution, formalin has to be eliminated in countries and regions with high environment-friendly standards, such as the medical college of hong Kong university and medical colleges of some countries in southeast Asia, and human pathological tissues are preserved by a freezing method with high energy consumption.
Therefore, the technicians in the field are dedicated to develop a non-formaldehyde environment-friendly pathological tissue fixative which can completely replace formalin solution and simultaneously overcome the problems of pungent smell, volatilization of effective components, poor softness of preserved pathological tissues, poor tissue fixing effect and the like of the traditional fixative.
Disclosure of Invention
In view of the above-mentioned defects of the prior art, the technical problem to be solved by the present invention is to provide a non-formaldehyde environment-friendly pathological tissue fixative to completely replace formalin solution, and simultaneously overcome the problems of pungent odor, volatilization of effective components, poor softness of preserved pathological tissues, poor tissue fixing effect, etc. of the traditional fixatives, and simultaneously create a good working environment for medical workers, and provide high-quality medical pathological tissues for the medical workers, which will help medical education and scientific research to develop better and faster.
In order to achieve the purpose, the invention provides a non-formaldehyde environment-friendly pathological tissue fixing agent which is characterized by comprising plant tannin and a buffer solution, wherein the content of tannin in the plant tannin is more than 8%, the buffer solution is a weak acid solution, the buffer solution is a mixed solution of sodium hydroxide, citric acid, urea, phosphoric acid and deionized water, the mass fraction of the plant tannin is 2% -10%, the mass fraction of sodium hydroxide is 0.5% -1%, the mass fraction of citric acid is 0.865% -3.5%, the mass fraction of urea is 0.045% -0.2%, the volume fraction of phosphoric acid is 0.2% -0.48%, and the balance is deionized water.
Further, the pH value of the buffer solution is 4.0-5.0.
Further, the plant tannin is 1kg, the mass of the buffer solution is 50kg, wherein 374g of sodium hydroxide, 945g of citric acid, 50g of urea, 203mL of phosphoric acid and the balance of deionized water.
Further, the plant tannin is 2kg, the mass of the buffer solution is 50kg, wherein the buffer solution comprises 296g of sodium hydroxide, 865g of citric acid, 45g of urea, 195mL of phosphoric acid and the balance of deionized water.
Further, the plant tannin is 5kg, the mass of the buffer solution is 200kg, wherein 595g of sodium hydroxide, 1730g of citric acid, 90g of urea, 350mL of phosphoric acid and the balance of deionized water are used.
Further, 10kg of plant tannin, 500kg of buffer solution, 1000g of sodium hydroxide, 3500g of citric acid, 200g of urea, 800mL of phosphoric acid and the balance of deionized water.
Further, the plant tannin is 20kg, the mass of the buffer solution is 1000kg, wherein the buffer solution comprises 2000g of sodium hydroxide, 7000g of citric acid, 400g of urea, 1800mL of phosphoric acid, and the balance of deionized water.
Further, the fixing agent is colorless transparent liquid, has no pungent smell and contains no volatile substances.
Furthermore, the fixing agent has excellent sterilization and mildew prevention functions and has the effects of resisting the formation of thrombus substances and dissolving thrombus.
Furthermore, the fixing agent is green and environment-friendly, and the shelf life of the fixing agent is more than 10 years.
Compared with the prior art, the non-formaldehyde environment-friendly pathological tissue fixing agent has the following positive effects:
(1) the invention can completely replace formalin solution, and simultaneously overcome the problems of pungent smell, volatile active ingredients, poor softness of preserved pathological tissues, poor tissue fixing effect and the like of the traditional fixative;
(2) the non-formaldehyde environment-friendly pathological tissue fixing agent is a multi-component matching system, ensures proper fixation of pathological tissues while sterilizing and preventing mildew, and has the advantages of no formaldehyde, no pungent smell, environmental protection, green color, transparent solution, non-volatile effective components, long drug effect, safety, high efficiency and excellent permeability;
(3) the invention solves the problem of formaldehyde which troubles medical workers for more than 100 years, creates a good working environment for the medical workers, provides high-quality medical pathological tissues for the medical workers, and promotes better and faster development of medical education and scientific research.
(4) The non-formaldehyde environment-friendly pathological tissue fixing agent is the only domestic environment-friendly product with a fixing effect, fundamentally improves the working teaching environment, improves the quality of pathological tissue preservation and fixation, and generates subversive innovation in the fields of biology, medicine and the like.
Detailed Description
The following describes several preferred embodiments of the present invention to make the technical contents thereof clearer and easier to understand. The present invention may be embodied in many different forms of embodiments and the scope of the invention is not limited to the embodiments set forth herein.
Example 1
Dissolving 1kg of plant tannin with tannin content more than or equal to 8% in 50kg of buffer solution with pH of 4.0, wherein the buffer solution is prepared by dissolving 374g of sodium hydroxide, 945g of citric acid, 50g of urea and 203ml of phosphoric acid in deionized water and diluting the mixture to 50kg, and finally obtaining 50kg of environment-friendly pathological tissue fixing agent with pH of 4.0.
Example 2
2kg of plant tannin with the tannin content of more than or equal to 8 percent is dissolved in 50kg of buffer solution with the pH value of 4.5, wherein the buffer solution is prepared by dissolving 296g of sodium hydroxide, 865g of citric acid, 45g of urea and 195ml of phosphoric acid in deionized water and diluting the mixture to 50kg, and finally 50kg of environment-friendly pathological tissue fixing agent with the pH value of 4.5 is obtained.
Example 3
Dissolving 5kg of plant tannin with tannin content more than or equal to 8% in 200kg of buffer solution with pH of 5.0, wherein the buffer solution is prepared by dissolving 595g of sodium hydroxide, 1730g of citric acid, 90g of urea and 350ml of phosphoric acid in deionized water to dilute 200kg, and finally obtaining 200kg of environment-friendly pathological tissue fixing agent with pH of 4.5.
Example 4
Dissolving 10kg of plant tannin with tannin content more than or equal to 8% in 500kg of buffer solution with pH value of 5.0, wherein the buffer solution is prepared by dissolving 1000g of sodium hydroxide, 3500g of citric acid, 200g of urea and 800ml of phosphoric acid in deionized water and diluting to 200kg, and finally obtaining 400 kg of environment-friendly pathological tissue fixing agent with pH value of 4.5.
Example 5
Dissolving 20kg of plant tannin with the tannin content of more than or equal to 8% in 1000kg of buffer solution with the pH value of 5.0, wherein the buffer solution is prepared by dissolving 2000g of sodium hydroxide, 7000g of citric acid, 400g of urea and 1800ml of phosphoric acid in deionized water and diluting the mixture to 200kg, and finally obtaining 800 kg of the environment-friendly pathological tissue fixing agent with the pH value of 4.5.
The above 5 examples are all developed based on plant tannin to obtain the non-formaldehyde environment-friendly pathological tissue fixing agent, the main difference lies in the difference of the component proportion, the comparison of the pathological tissue preservation effect of the invention and the existing pathological tissue fixing agent on the market is made below to illustrate the benefits of the invention, the application effect and the like products are compared as shown in the following table:
TABLE 1 demonstration of the pathological tissue preservation Effect
From the evaluation criteria for the preservation effect of the pathological tissues shown in Table 1, we can conclude, by comprehensive analysis, that: the non-formaldehyde environment-friendly pathological tissue fixing agent developed based on the plant tannin has the advantages of optimal preservation effect, good transparency of the fixing agent, no precipitation of the fixing agent, no mildew of pathological tissues and no change of color of the pathological tissues.
TABLE 2 demonstration of pathological tissue preservation Effect
From the evaluation criteria in table 2 demonstrating the preservation effect of pathological tissues, we can conclude, by comprehensive analysis: the non-formaldehyde environment-friendly pathological tissue fixing agent developed based on the plant tannin has the best effect, does not stimulate cornea, respiratory tract and skin, does not harm human bodies, teaches that the pathological tissues do not have the phenomena of rot, putrefaction and the like, and does not change the color and luster of the pathological tissues.
TABLE 3 comparison of Effect of the existing fixatives at home and abroad
Table 3 comparative analysis of the effect of the present fixative in and outside of china can give: the non-formaldehyde environment-friendly pathological tissue fixing agent developed based on the plant tannin has good permeability and fixity, and compared with other fixing agents, the non-formaldehyde environment-friendly pathological tissue fixing agent also has a certain price advantage; the plant tannin is a green and environment-friendly organic phosphine material, has higher antimicrobial property, low toxicity, easy degradation, no residue and no harm to human bodies, so the invention has good environment-friendliness.
The foregoing detailed description of the preferred embodiments of the invention has been presented. It should be understood that numerous modifications and variations could be devised by those skilled in the art in light of the present teachings without departing from the inventive concepts. Therefore, the technical solutions available to those skilled in the art through logic analysis, reasoning and limited experiments based on the prior art according to the concept of the present invention should be within the scope of protection defined by the claims.
Claims (8)
1. The non-formaldehyde environment-friendly pathological tissue fixing agent is characterized by comprising plant tannin and a buffer solution, wherein the content of tannin in the plant tannin is more than 8%, the buffer solution is a weak acid solution, and the buffer solution is a mixed solution of sodium hydroxide, citric acid, urea, phosphoric acid and deionized water, wherein the mass fraction of the plant tannin is 2% -10%, the mass fraction of the sodium hydroxide is 0.5% -1%, the mass fraction of the citric acid is 0.865% -3.5%, the mass fraction of the urea is 0.045% -0.2%, the volume fraction of the phosphoric acid is 0.2% -0.48%, and the balance is the deionized water.
2. The non-formaldehyde environmentally friendly pathological tissue fixative according to claim 1, wherein preferably the buffer solution has a pH of 4.0 to 5.0.
3. The non-formaldehyde environment-friendly pathological tissue fixing agent as claimed in claim 1, wherein the plant tannin accounts for 1kg, the mass of the buffer solution is 50kg, the sodium hydroxide accounts for 374g, the citric acid accounts for 945g, the urea accounts for 50g, the phosphoric acid accounts for 203mL, and the rest is deionized water.
4. The non-formaldehyde environment-friendly pathological tissue fixing agent as claimed in claim 1, wherein the plant tannin is 2kg, the mass of the buffer solution is 50kg, and the buffer solution comprises 296g of sodium hydroxide, 865g of citric acid, 45g of urea, 195mL of phosphoric acid, and the balance of deionized water.
5. The non-formaldehyde environment-friendly pathological tissue fixing agent as claimed in claim 1, wherein the plant tannin is 5kg, the mass of the buffer solution is 200kg, and the buffer solution comprises 595g of sodium hydroxide, 1730g of citric acid, 90g of urea, 350mL of phosphoric acid, and the balance of deionized water.
6. The non-formaldehyde environment-friendly pathological tissue fixing agent as claimed in claim 1, wherein the plant tannin is 10kg, the mass of the buffer solution is 500kg, and the buffer solution comprises 1000g of sodium hydroxide, 3500g of citric acid, 200g of urea, 800mL of phosphoric acid and the balance of deionized water.
7. The non-formaldehyde environment-friendly pathological tissue fixing agent as claimed in claim 1, wherein the plant tannin is 20kg, the mass of the buffer solution is 1000kg, and the buffer solution comprises 2000g of sodium hydroxide, 7000g of citric acid, 400g of urea, 1800mL of phosphoric acid, and the balance of deionized water.
8. The non-formaldehyde environmentally friendly pathological tissue fixative of claim 1, wherein the fixative is a colorless transparent liquid, free of pungent odor and volatile substances.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810616429.1A CN110604126A (en) | 2018-06-14 | 2018-06-14 | Non-formaldehyde environment-friendly pathological tissue fixing agent |
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| CN201810616429.1A CN110604126A (en) | 2018-06-14 | 2018-06-14 | Non-formaldehyde environment-friendly pathological tissue fixing agent |
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| CN110604126A true CN110604126A (en) | 2019-12-24 |
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Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101545876A (en) * | 2009-04-29 | 2009-09-30 | 中国海洋大学 | Method for observing collagenous fibre of fresh and alive trepang with electron microscope |
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| CN105392363A (en) * | 2013-03-01 | 2016-03-09 | A·S·戈尔兹伯勒 | Sample fixation and stabilisation |
| CN104338180A (en) * | 2013-07-31 | 2015-02-11 | 百多力股份公司 | Method for preparing biological tissue |
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