RU2363158C1 - Biocidal composition for soaking napkins - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: biocidal composition contains benzalkonium chloride with chain length C₁₂-C₁₆ as a quaternary ammonium compound and also a polyguanidine - polyhexamethyleneguanidine phosphate compound, or poly-(4,9-dioxadodecaneguanidine) chloride, or poly-(4,9-dioxadodecaneguanidine) phosphate, or polyhexamethyleneguanidine succinate, or poly-(4,9-dioxadodecaneguanidine) succinate, oxyethylated sorbitan laurate, polyethylene glycol-7 - glycerol cocoate, camomile extract, great stonecrop aqueous extract, lactic acid and allantoin in defined ratio of components in wt %.

EFFECT: increased biocidal effectiveness, soothing effect on skin and anti-inflammatory properties.

3 tbl, 5 ex

Description

The invention relates to the field of hygiene and sanitation, and in particular to compositions for the impregnation of napkins.

The composition is used to disinfect the skin of the hands, feet, face and other parts of the body and can be used in everyday life and medicine.

The stratum corneum is the uppermost layer of the skin, provides regulation of the water content in the skin and acts as a barrier to protect against chemicals and other stressful environmental factors. The complex distribution of lipids in the intercellular space of the stratum corneum provides the creation of a normal barrier function. Multilayer structures consisting of cholesterol, ceramides and fatty acids, as well as small amounts of some other lipids, create the main barrier to the transfer of hydrophilic substances into the skin and through the skin. The relationship between barrier function and skin condition can be seen with skin irritation, which is a consequence of lipid extraction from the skin.

In addition to irritation, a violation of the barrier function of the skin can be caused by a number of pathological conditions of the skin, the most expressive example of which is dry skin. For the treatment of dry skin, moisturizers are most often used, which contain chemicals that clog the skin and moisture-retaining substances.

The body tends to naturally cure impaired skin barrier function by enhancing the production of essential lipids contained in the skin, such as cholesterol, ceramides and fatty acids, and essential lipid-synthesizing enzymes.

Moisturizing wipes can help restore the balance of the skin to the body, which, in addition to the moisturizing effect, can create the effect of protecting the skin from external microorganisms.

This occurs if a disinfectant is present in the impregnation composition of the wipes.

A technical solution is known in which moisturizing wipes have the ability to improve the condition of the skin. The impregnation for such wipes is an oil-in-water composition containing alcohol, natural fat or oil, stearin, a water-retaining substance, an emulsifying surfactant and water (US Pat. RF No. 2246319, class A61L 15/34, Bull. No. 5, 2005).

However, the known solution has a limited range of action on external microorganisms, because alcohol destroys a narrow group of bacteria.

The closest technical solution to the proposed solution is a VELTALEX disinfectant for wipes, including ethyl alcohol, glycerin, auxiliary components, didecyldimethylammonium bromide clathrate and water (Pat. RF No. 2132698, class A61L 2/16, Bull. No. 19 , 1999).

The known composition is used to disinfect the skin of the hands, feet, face and other parts of the body.

The disadvantages of the known disinfectant composition for the impregnation of napkins include insufficient antimicrobial activity, as well as low consumer properties due to the evaporation of the alcohol component from the surface of the napkin.

The problem solved by the invention is to increase biocidal activity, increase the range of action on microorganisms, improve the consumer properties of the composition.

To solve the problem in a biocidal composition for the impregnation of napkins, including a quaternary ammonium compound, glycerin, perfume and distilled water, benzalkonium chloride with a chain length of C12-C16 is used as a quaternary ammonium compound (QA), and it additionally contains a polyguanidine-phosphate compound polyhexamethylene guanidine, or poly- (4,9-dioxadodecanguanidine) chloride, or poly- (4,9-dioxadodecanguanidine) phosphate, or polyhexamethylene guanidine succinate, or poly- (4,9-dioxadodecangane succinate uanidine), ethoxylated sorbitan laurate, PEG-7 glyceryl cocoate, chamomile extract, aqueous extract of stonecrop large, lactic acid, allantoin in the following ratio of components in wt.%:

polyguanidine compound 0.1-1.0 benzalkonium chloride with a chain length of C12-C16 0.05-0.3 glycerol 0.5-1.0 ethoxylated sorbitan laurate 0.2-1.0 PEG-7 glyceryl cocoate 0.2-1.0 chamomile extract 0.05-0.5 large stonecrop water extract 0.05-1.0 lactic acid 0.1-0.5 allantoin 0.1-0.5 perfume perfume 0.05-0.2 distilled water rest

The invention is illustrated as follows.

The highly effective polyguanidine compounds used in the napkin impregnation composition have a wide range of biocidal activity.

As polyguanidine compounds, phosphosexamethylene guanidine phosphate (PHMG) “FOSFOPAG”, poly- (4,9-dioxadodecanguanidine chloride (PDDH) “ECOSEPT”, poly- (4,9-dioxadeodecanguanidine phosphate) (ECOGecUnat PECGAT, ECOMEPAT PDDG.

These compounds are produced by the NGO Institute of Ecological and Technological Problems. The trademarks "FOSFOPAG" and "ECOSEPT" belong to the NGO "Institute of Ecological and Technological Problems".

Most preferred is the use of PGMG succinate and PDDG succinate.

PGMG succinate (disubstituted) has the following structural formula:

n = 30-50.

The poly- (4,9-dioxadodecanguanidine) succinate (disubstituted) has the following structural formula:

n = 30-50.

Succinic acid, which is part of PHMG succinate and poly- (4,9-dioxadodecanguanidine) succinate, is a bioactive natural compound that is present in the human body, and therefore, the succinic acid salt is necessary for the human body. The biocidal effect of the napkin impregnation composition is enhanced due to the fact that it is PHMG succinate and poly- (4,9-dioxadodecanguanidine) succinate that contribute to the longer-term effect of the napkin impregnation composition after contact with skin.

Benzalkonium chloride with a chain length of C12-C16 is used as an HOUR.

This is due to the fact that among mono-quaternary ammonium salts, the maximum activity, as a rule, is exhibited by compounds that contain 12-16 carbon atoms in the radical.

where n = 12-16.

Supplier SBK was founded in 1997 on the basis of the Russian representative office of the raw materials division of the German chemical concern Henkel KgaA (currently Cognis Deutshland GmbH) and specializes in the supply of raw materials for various branches of the chemical industry.

When polyguanidine is connected with QAS, a synergistic mixture is formed that has a wide range of biocidal activity: bactericidal (effective against Pseudomonas aeruginosa, staphylococci, Salmonella, pathogens of dysentery, diphtheria, Legionella), virucidal (HIV infection, hepatitis, herpes, influenza), fungi effective against dermatophytosis, candida albicans, aspergillus niger and other mold fungi).

Glycerin (TU 6259-25) is a clear liquid. Glycerin is an enveloping and astringent component. It has a softening and moisturizing effect on the skin, promotes the penetration of active substances into the skin, activates metabolic processes. The use of glycerin increases the effectiveness of the proposed biocidal composition.

The ethoxylated sorbitan laurate (trademark KAOPAN TWL-120, manufactured by Kao Chemicals Europe) is used as a dermatologically mild surfactant to enhance the cleansing properties of the biocidal composition.

PEG-7 glyceryl cocoate (trademark EMANON NOT, manufactured by Kao Chemicals Europe) is used as an additional mild cleansing surfactant (co-surfactant), which has the ability to soften and moisturize the skin, as well as increase the homogeneity of the biocidal composition.

Propylene glycol chamomile extract (preferably produced by Kamelia NPP LLC, Moscow or Pluton Farm CJSC, Elektrostal) is used as a component with antimicrobial and anti-inflammatory effects.

The aqueous extract of stonecrop large has wound healing properties, enhances metabolic processes in tissues and their regeneration, has a tonic and anti-inflammatory effect on the skin.

The extract is prepared as follows.

150 g of raw material - (leaves and roots of a plant) are placed in a container (8-10 l), 6 l of boiling water is poured and insisted for 4 hours, after which it is filtered. About 5.5 L of extract is obtained.

Lactic acid (hydroxypropionic acid) CH ₃ CH (OH) COOH gross formula C ₃ H ₆ O ₃ , M = 90 (GOST 490-79), _mp = 25-26 ° C, soluble in water, alcohol, glycerin. It is formed during lactic fermentation of sugary substances under the action of bacteria. It is used in the manufacture of medicines. Known for its antiviral effect.

Allantoin has a calming effect on the skin, stimulates tissue regeneration, removes dead epithelial cells. Allantoin is found in the roots of the plant Symphytum officinale (Comfrey officinalis). Allantoin manufactured by RESPHARMA (Italy) is used.

Perfumery fragrance is an organic liquid with a characteristic perfume smell. Use various fragrances with fruit and flower, fruit and herbal, spicy, coniferous aromas.

Most preferred are fragrances of Balsam OJSC (RF, Nizhny Novgorod) or CPL Aromas LTD (England, supplier of NovaCom CJSC, RF, Moscow).

As a basis in the composition, distilled water is used (GOST 609-84).

The quantitative content of the components of the biocidal composition recommended by the authors is optimal and is selected on the basis of numerous experiments.

Table 1 presents data confirming the optimality of the claimed ratios of the components of the composition, as well as data that goes beyond the stated ratios.

Alternative features of claim 1 of the claims provide the achievement of the same technical problem as given in table 1.

The process of preparing the biocidal composition includes the preparation of raw materials and equipment, the weighing of components. Separately, a solution of the polyguanidine and QAC compound is prepared by dissolving in water, an aqueous solution of a large stonecrop is also obtained in a separate container.

In practice, the preparation of the biocidal solution is carried out as follows: distilled water is pumped into the reactor, and ethoxylated sorbitan laurate and PEG-7 glyceryl cocoate are added. After that, turn on the mixing mode for 10 minutes until the solution is completely homogenized at a temperature of 30-35 ° C. Mixing speed 70 rpm

When the stirrer is working through the hatch, a solution of the polyguanidine and QAS compound, glycerin, chamomile extract, an aqueous solution of the stonecrop extract, lactic acid, allantoin, perfume composition and the missing amount of water are added in accordance with the recipe.

The resulting mixture is stirred for 5 minutes, after which sampling is carried out to check the external indicators (color, smell and compliance with the technical specifications).

The resulting biocidal composition is pumped into a polymer container.

Examples of specific biocidal composition.

Example No. 1.

The biocidal composition for napkin impregnation contains the following components, wt.%:

polyguanidine compound - PHMG phosphate 0.1 benzalkonium chloride with a chain length of C ₁₂ -C ₁₆ 0.05 glycerol 0.5 ethoxylated sorbitan laurate 1,0 PEG-7 glyceryl cocoate 0.2 chamomile extract 0.5 large stonecrop water extract 0.5 lactic acid 0.3 allantoin 0.3 perfume perfume 0.05 distilled water rest

The composition is a transparent liquid, the use of which protects the skin from the penetration of pathogenic microbes and bacteria. It has a moisturizing effect, does not upset the pH balance.

Example No. 2.

The biocidal composition for napkin impregnation contains the following components, wt.%:

polyguanidine compound - PDDG chloride 0.5 benzalkonium chloride with a chain length of C ₁₂ -C ₁₆ 0.3 glycerol 1,0 ethoxylated sorbitan laurate 0.6 PEG-7 glyceryl cocoate 1,0 chamomile extract 0.05 large stonecrop water extract 0.7 lactic acid 0.5 allantoin 0.5 perfume perfume 0.1 distilled water rest

Transparent liquid with a prolonged antimicrobial effect. It cleanses the skin well, has a regenerating effect, does not dry the skin, does not upset the pH balance.

Example No. 3.

The biocidal composition for napkin impregnation contains the following components, wt.%:

polyguanidine compound - PDDG succinate 1,0 benzalkonium chloride with a chain length of C ₁₂ -C ₁₆ 0.1 glycerol 0.7 ethoxylated sorbitan laurate 0.2 PEG-7 glyceryl cocoate 0.5 chamomile extract 0.2 large stonecrop water extract 1,0 lactic acid 0.1 allantoin 0.1 perfume perfume 0.2 distilled water rest

Transparent liquid, the use of which allows for long-term skin protection against pathogenic microbes and fungal infections. Eliminates irritation, dryness, peeling of the skin, does not violate the pH balance.

Example No. 4.

The biocidal composition for napkin impregnation contains the following components, wt.%:

polyguanidine compound - PGMG succinate 0.5 benzalkonium chloride with a chain length of C ₁₂ -C ₁₆ 0.2 glycerol 0.5 ethoxylated sorbitan laurate 0.6 PEG-7 glyceryl cocoate 1,0 chamomile extract 0.2 large stonecrop water extract 0.7 lactic acid 0.1 allantoin 0.1 perfume perfume 0.2 distilled water rest

Transparent liquid for a long time ensures safety from pathogenic microbes. Gently cleanses, moisturizes and refreshes the skin.

Example No. 5.

The biocidal composition for napkin impregnation contains the following components, wt.%:

polyguanidine compound - PDDH phosphate 0.3 benzalkonium chloride with a chain length of C ₁₂ -C ₁₆ 0.1 glycerol 0.7 ethoxylated sorbitan laurate 0.2 PEG-7 glyceryl cocoate 0.5 chamomile extract 0.1 large stonecrop water extract 1,0 lactic acid 0.3 allantoin 0.5 perfume perfume 0.15 distilled water rest

Transparent liquid, the use of which allows for prolonged protection of the skin from pathogenic microbes and fungal infections. It softens, eliminates irritation, peeling of the skin, promotes the rapid healing of cracks, abrasions on the skin.

When testing the antimicrobial activity of the developed biocidal composition for the impregnation of wipes, 2 methods were used. The first method was used to determine the zones of suppression of microorganisms during the diffusion of the test substance into a solid nutrient medium (Fedorov Yu.A., Koren V.N. Microbiological methods for the study of dental hygiene products in the book "Fundamentals of oral hygiene". L .: Medicine, 1973 ., p. 3-215).

For this, 20 ml of solid nutrient medium was poured into a Petri dish. A 24-hour microbial suspension of test microorganisms with a concentration of 5 × 10 ⁵ CFU / ml was prepared and 1 ml of suspension was applied to the surface of the nutrient medium. Dried for 30 minutes, and then in a nutrient medium made holes with a diameter of 6 mm, which were applied samples of the developed biocidal composition for the impregnation of napkins. The number of holes should not exceed 6 per Petri dish. For 30 minutes, Petri dishes were kept at room temperature (20 ° C), then they were placed in a thermostat for 24-48 hours at a temperature of 37 ° C, and after this period, zones of inhibition of microbial growth, including the diameter of the wells, were taken into account. By the size of the zones of growth inhibition of test microorganisms, the antimicrobial effect of the test substance was judged, although it should be borne in mind that the diffusion rate of polymer disinfectants is lower than the diffusion rate of low polymer disinfectants.

To obtain reliable test results for each test substance, it is necessary to carry out in 2-3 series of replicates.

The second way to test the antimicrobial activity of the biocidal composition for the impregnation of wipes is to determine the minimum time required to completely suppress the growth and development of test microorganisms.

A suspension of test microorganisms was added to the test substance in an amount of 10 ⁵ CFU / ml; for this, 1 ml of the test substance was poured into test tubes. The tubes were shaken and, at intervals of 2-5-10 and 30 minutes, were sown on solid nutrient media. Petri dishes were placed for 24-48 hours in a thermostat at 37 ° C. After this period, the number of colonies grown was counted and the bactericidal activity of the test substance was judged by this indicator.

The results of the studies are presented in table.2.

From the data presented in table 2, it follows that gram-positive microorganisms (S. aureus) were more sensitive to the tested biocidal compositions for the impregnation of napkins. All tested biocidal compositions formed growth retardation zones from 20 to 38 mm, which corresponds to a fairly high activity. Noteworthy is the high activity of the biocidal composition of Example 3 to gram-negative microorganisms of the species E. coli and Ps.aeruginosa.

When determining the rate of manifestation of antimicrobial activity to the tested biocidal compositions, the results were obtained, are presented in table 3.

From the data presented in Table 3, it follows that the antibacterial effect of the developed biocidal compositions develops very quickly. After 2 minutes of contact, in no case was live microflora detected after contamination of the nutrient medium with Escherichia coli.

Tests of the biocidal composition for the impregnation of napkins were carried out in an accredited specialized Center for volunteers. A highly effective effect of the biocidal composition on the skin is noted, it is shown that the biocidal composition is harmless, does not have an irritating and allergenic effect.

table 2 Samples of the biocidal composition for the impregnation of napkins Microorganism growth inhibition zones, mm S.aureus E.coli Ps.aeruginosa Example 1 28 twenty 24 Example 2 34 25 27 Example 3 38 24 26 Example 4 34 27 22 Example 5 thirty 21 26 Table 3 Samples of the biocidal composition for the impregnation of napkins Seeding * after a period of time (min) 2 5 10 thirty Example 1 not upd. not upd. not upd. not upd. Example 2 not upd. not upd. not upd. not upd. Example 3 not upd. not upd. not upd. not upd. Example 4 not upd. not upd. not upd. not upd. Example 5 not upd. not upd. not upd. not upd. * bacteria of the species E. coli, 10 ⁵ CFU / cm ³

Claims (1)

A biocidal composition for impregnating napkins, including a quaternary ammonium compound, glycerin, perfume and distilled water, characterized in that benzalkonium chloride with a chain length of C ₁₂ -C _{16 is} used as a quaternary ammonium compound and additionally contains a polyguanidine-polyhexamethylene guanidine or poly- (4,9-dioxadodecanguanidine), or poly- (4,9-dioxadodecanguanidine) phosphate, or polyhexamethylene guanidine succinate, or poly- (4,9-dioxadodecanguanidine succinate), hydroxyethyl ovany sorbitan laurate, polyethylene glycol-7 glyceryl cocoate, chamomile extract, aqueous extract of stonecrop large, lactic acid and allantoin in the following ratio, wt.%:
polyguanidine compound 0.1-1.0 benzalkonium chloride C ₁₂ -C ₁₆ 0.05-0.3 glycerol 0.5-1.0 ethoxylated sorbitan laurate 0.2-1.0 polyethylene glycol-7 glyceryl cocoate 0.2-1.0 chamomile extract 0.05-0.5 large stonecrop water extract 0.05-1.0 lactic acid 0.1-0.5 allantoin 0.1-0.5 perfume perfume 0.05-0.2 distilled water rest