CN104798793A - Germicidal suspending agent containing tetramycin and difenoconazole and preparation method of germicidal suspending agent - Google Patents
Germicidal suspending agent containing tetramycin and difenoconazole and preparation method of germicidal suspending agent Download PDFInfo
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- CN104798793A CN104798793A CN201510200281.XA CN201510200281A CN104798793A CN 104798793 A CN104798793 A CN 104798793A CN 201510200281 A CN201510200281 A CN 201510200281A CN 104798793 A CN104798793 A CN 104798793A
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- difenoconazole
- tetramycin
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Abstract
The invention relates to the technical field of pesticide compounding, in particular to a germicidal suspending agent containing tetramycin and difenoconazole and a preparation method of the germicidal suspending agent. The germicidal suspending agent containing tetramycin and difenoconazole has the effective constituents of tetramycin and difenoconazole which are subjected to binary combination, and comprises the following constituents in parts by mass: 0.1-25 parts of tetramycin, 0.1-25 parts of difenoconazole, 5-20 parts of a dispersant, 1-5 parts of an anti-freezing agent, 0.1-2 parts of a thickening agent, 0.1-0.8 part of a foam killer, 0-10 parts of a penetration enhancer, 0.1-5 parts of a pH value regulator and the balance of the water. The germicidal suspending agent is used for preventing and controlling various funguses and bacterial diseases of various crops, mainly bacterial angular leaf spot disease, anthracnose, scab, powdery mildew, rust disease and the like.
Description
Technical field
The present invention relates to technical field of pesticide combination, particularly relate to a kind of bactericidal suspending agent containing tetramycin and Difenoconazole and preparation method thereof.
Background technology
The fermentating metabolism product that tetramycin (tetramycin) is S. ahygroscopicus Wuzhou subspecies, fungicidal spectrum is wide, all has extremely strong killing action to three main kind of 26 kinds of known disease funguses such as mastigomycetes, sac fungi and Deuteromycotina fungies.Be suitable for the multiple fungi of various crop, the control of bacteriosis.Especially to fruit tree putrefaction disease spot defoliation; Rice blast; Root rot; Cucurbits fusarium wilt; Cotton verticillium wilt; Jujube tree rust; Fruit white rot of grape; Panax pseudoginseng black spot; Tealeaves tea gall, the fungal disease special efficacys such as forest rot, canker, gummosis, defoliation, woods seedling blight.
Difenoconazole is triazole bactericidal agent; fungicidal spectrum is wide; to Ascomycetes, Basidiomycetes and comprise Alternaria, Ascochyta, the mould genus of tail spore, Colletotrichum, ball Cuo Pseudomonas, Phoma, Ramularia, Septoria, Venturia partly know disease, Erysiphaceae, Uredinales and some Seedborne pathogen have lasting protection and therapeutic action.Also fine to bitter rot or anthracnose of grape, white rot effect.Leaf surface treatment or seed treatment can improve output and the guaranteed quality of crop.
In the real process of agricultural production, using chemical agent is the most effective means of control plant pest, but is the generation of disease drug resistance by the problem that chemical control disease the most easily produces.In the chemical control of bacteriosis, years of researches are carried out both at home and abroad, although some antibacterium medicaments have started to enter practical stage, but the medicament of chemosynthesis has cycle long, the shortcoming such as cost is high, toxicity is large, environmental pollution is serious, can not meet the requirement of agricultural sustainable development far away.And long-term use single pesticidal preparations continuously high dose, easily cause the problems such as the remaining of medicament, environmental pollution and resistance to pesticide resistance fungi development.And by contrast, exploitation is efficient with research, low toxicity, low-residual composite and mixture has small investment, the lead time is short and be subject to domestic and international attention, numerous and confused increasing develops dynamics.We are on the basis of lab screening and field trial, filter out tetramycin and Difenoconazole carries out composite, have obvious synergistic effect.And about tetramycin and Difenoconazole composite composition pesticide and apply and there is no people at present and reported.
Summary of the invention
Based on above situation, the object of the invention is to provide a kind of new and effective pesticide sterilizing suspending agent, for the multiple fungi of various crop, the control of bacteriosis, be mainly used in the diseases such as control bacterial angular leaf spot, anthracnose, scab, powdery mildew, rust.
Technical scheme of the present invention is realized by following measures:
Contain a bactericidal suspending agent for tetramycin and Difenoconazole, the active ingredient of this bactericidal suspending agent is tetramycin and Difenoconazole binary built, comprises following component by mass fraction: tetramycin 0.1 ~ 25 part; Difenoconazole 0.1 ~ 25 part; Dispersant 5 ~ 20 parts; Antifreezing agent 1 ~ 5 part; Thickener 0.1 ~ 2 part; Defoamer 0.1 ~ 0.8 part; Penetrating agent 0 ~ 10 part; PH value conditioning agent 0.1 ~ 5 part; Water, surplus.
As preferably, the mass ratio of tetramycin and Difenoconazole is 1 ~ 10: 50 ~ 1.
As preferably, the mass ratio of tetramycin and Difenoconazole is 1:10 ~ 10:1.
As preferably, it is LG-3, GY-D1252, GY-D1256, SNWGF-01 that dispersant is selected from polycarboxylate code name, lignosulfonates code name is 201107,201108, alkylphenol polyoxyethylene methyl ether condensate sulfate, alkylsulfonate calcium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, alkylphenol polyoxyethylene, polyoxyethylene carboxylate, aliphatic amine polyoxyethylene ether, one or more in fatty acid glyceride APEO.
As preferably, described antifreezing agent is selected from ethylene glycol, propane diols, one or more in glycerine.
As preferably, described defoamer is selected from silicone oil, silicone compound, C10-20 saturated fat acid compounds, one or more of C8-10 fatty alcohol.
As preferably, described thickener is selected from xanthans, carboxymethyl cellulose, carboxyethyl cellulose, methylcellulose, Magnesiumaluminumsilicate, in polyvinyl alcohol one or more.
A kind of preparation method preparing described bactericidal suspending agent, first other auxiliary agents are mixed, mix through high speed shear, add active ingredient tetramycin and Difenoconazole, grind 2 ~ 3 hours in sand mill, make a diameter all at below 5mm, namely can be made into the aqueous suspension formulations of the present composition.
The composite bactericide that the present invention is active ingredient with tetramycin and Difenoconazole has obvious synergistic effect, delays the drug-fast generation in key, and reduces into product cost and use cost, can be used for the improvement of resistance disease.For the multiple fungi of various crop, the control of bacteriosis, be mainly used in the diseases such as control cucumber bacterial angular leaf spot, anthracnose, scab, powdery mildew, rust.
Embodiment
For making technical scheme of the present invention, object and advantage are clearly understood, the following specific embodiment of the present invention is described, but the present invention is not limited to these examples.The mode that effect experimental of the present invention adopts indoor biometrics and field trial to combine, if no special instructions, the ratio below mentioned is all ratio of quality and the number of copies.
Embodiment 1:
Contain a bactericidal suspending agent for tetramycin and Difenoconazole, the active ingredient of this bactericidal suspending agent is tetramycin and Difenoconazole binary built, comprises following component by mass fraction: tetramycin 10 parts; Difenoconazole 5 parts; Dispersant 10 parts; Antifreezing agent 2 parts; Thickener 0.5 part; Defoamer 0.5 part; Penetrating agent 2 parts; PH value conditioning agent 0.5 part; Water, surplus.
First other auxiliary agents are mixed, mix through high speed shear, add active ingredient tetramycin and Difenoconazole, grind 2 ~ 3 hours in sand mill, make a diameter all at below 5mm, namely can be made into the aqueous suspension formulations of the present composition.
embodiment 2: tetramycin and Difenoconazole different ratio co-toxicity are tested.
1.1 test targets
Cucumber bacterial angular leaf spot bacterium (germ bacterial classification picks up from Pu Yan town, XiaoShan, HangZhou City, Zhejiang Province cucumber booth, obtains through the Sheng Ce laboratory separation and purification of research and development centre of Zhejiang Sega Science and Technology Co., Ltd.).
1.2 test method
The female medicine of 15% tetramycin, 95% difenoconazole technical material, is provided by Zhejiang Sega Science and Technology Co., Ltd..Add aqua sterilisa by purified slant tube bacterial classification, be made into former bacteria suspension, then be diluted to 107/rise bacteria suspension, for subsequent use.First by tetramycin with
1000mg/L mother liquor is made into, again with sterile water dilution (unit: mg/litre) during use after the water-soluble solution of the female medicinal sterile of Difenoconazole.
Tetramycin is mixed with Difenoconazole sets 5 assembly ratios altogether, and on the basis of preliminary experiment, each single dose and each proportioning establish 5 concentration process respectively, not contain the system of liquid for blank (maximum concentration with reference to chemicals treatment dilutes).Each concentration process repeats 4 times, under aseptic technique, each concentration gradient medicament is drawn 4ml successively respectively from low concentration to high concentration, add in the aseptic conical flask melted in advance containing 36ml sterilising medium, fully shake up, then pour into immediately in the culture dish of diameter 9cm, make corresponding pastille dull and stereotyped.Every ware add 107/rise bacterial suspension 200 μ l, then with after sterilizing triangular glass rod bacteria suspension in ware is pushed away even, be placed in 28 DEG C of insulating boxs and cultivate.Separately establish the process not containing medicament as blank.Each process repetition 4 times.
1.3 investigation method
Cultivate after 48 hours and culture dish is taken out, according to blank, observe bacteria colony count in each ware of each concentration.
Relative inhibition is calculated according to each process bacteria colony count:
Relative inhibition (%)=(blank clump count-chemicals treatment clump count) * 100%/blank clump count
1.4 synegism evaluation
The synergistic effect of pharmacy mix is evaluated according to the co-toxicity coefficient method (CTC) of Sun & Johnson (1960), namely CTC≤80 are antagonism, 80 < CTC < 120 are summation action, and CTC >=120 are synergistic effect.
Actual measurement toxicity index (ATI)=standard agent EC50/ reagent agent EC50*100
In toxicity index * 100 mixture of theoretical toxicity index (the TTI)=medicament A of mixture the percentage composition+medicament B of A medicament toxicity index * 100 mixture in the percentage composition of B medicament
The theoretical toxicity index (TTI) * 100 of co-toxicity coefficient (CTC)=mixture actual measurement toxicity index (ATI)/mixture.
2.1 toxicity test results
Table 1 tetramycin and Difenoconazole are to the toxicity test of cucumber bacterial angular leaf spot bacterium
As can be seen from the table, the result of the test of different proportion proportioning shows, dilutes respectively all show in active ingredient ratio
Stronger synergistic effect, wherein with tetramycin: time Difenoconazole is 1:10 ~ 10:1, synergistic effect is best, suggestion carries out further field control effectiveness test to suitable proportion about 1:10 ~ 10:1 mixture preparation, to evaluate its field practical application effect.
3 field trials prevent and treat the experimental result of bitter rot or anthracnose of grape, cucumber bacterial angular leaf spot
3.1 field trials prevent and treat bitter rot or anthracnose of grape
3.1.1 test method
Test is located in Jinhua Academy of Agricultural Sciences proving ground beam-connected greenhouse.Experimental cultivar is that rattan harvests grape, the age of tree 4 years,
Seeding row spacing 1m × 4m, pergola system.This garden booth anniversary epiphragma, the sterilization of clear garden is not thorough, and in soil, invalid body is more, goes through
Over year, bitter rot or anthracnose of grape occurs more serious.Be experimental field faintly acid sandy loam, the content of organic matter is lower, and Fertilization Level is medium, and moisture is sufficient, and soil keeps moistening for a long time.
3.1.2 control time, method and number of times
Investigate incidence and disease index before dispenser, after medicine 10 days, second time medicine after within 10 days and 21 days, investigate, press
Tree position, three, body upper, middle and lower is each takes out 10 fruit ears at random and investigates disease level.Grade scale is as follows:
0 grade: anosis;
1 grade: sick portion accounts for less than 5% of whole fruit ear;
3 grades: sick portion accounts for the 6%-15% of whole fruit ear;
5 grades: sick portion accounts for the 16%-25% of whole fruit ear;
7 grades: sick portion accounts for the 26%-50% of whole fruit ear;
9 grades: sick portion accounts for more than 50% of whole fruit ear.
3.1.3 drug effect computational methods
Diseased fruit rate (%)=disease fruit number/investigation total fruit number × 100
Disease index=Σ (sick fruit number × relative level numerical value at different levels)/(investigation total fruit number × 9) × 100
Control efficiency (%)=(before 1-(blank district medicine after disease index × treatment region medicine disease index)/(blank
Disease index before disease index after district's medicine × treatment region medicine)) × 100.
3.1.4 poisoning investigation method
Whether after dispenser, continuous 10d estimates medicament has poisoning to crop.
3.1.5 result of the test and analysis
The effect of bitter rot or anthracnose of grape prevented and treated by table 2 respectively process.
Control successful higher than tetramycin and Difenoconazole single dose with 3:2 ratio composite bactericide for bitter rot or anthracnose of grape by the known tetramycin of table 2 and Difenoconazole, bactericidal effect increases progressively with the increase of dosage.According to field range estimation, within the scope of test dose, plant growth is normal, and the poisoning phenomenon to grape does not all appear in each treatment agent, illustrates that it is safe to grape, significant for agricultural produce, is worthy to be popularized.
3.2 field trial control cucumber bacterial angular leaf spots
3.2.1 test method
Test on Jiaxing City, Zhejiang Province cucumber ground, test kind is middle peasant No. 8, and often process 4 times and repeat, every community double diagonal line is adjusted
Look into 5 points, often some investigation 3 strain cucumber, record occurring degree by staging, grade scale is as follows:
0 grade: anosis;
1 grade: scab heap area accounts for less than 5% of whole leaf area;
3 grades: scab heap area accounts for below the 6%-10% of whole leaf area;
5 grades: scab heap area accounts for below the 11%-20% of whole leaf area;
7 grades: scab heap area accounts for below the 21%-50% of whole leaf area;
9 grades: scab heap area accounts for more than 50% of whole leaf area.
3.2.2 control time and number of times
Test altogether investigate 4 times, before medicine radix investigation, first time medicine after 7 days, second time 7 days and medicine after within 15 days, investigate.
3.2.3 drug effect computational methods
Sick leaf rate (%)=sick number of sheets/investigate the total number of sheets × 100
Disease index=Σ (the sick number of sheets × relative level numerical value at different levels)/(investigating the total number of sheets × 9) × 100
Control efficiency (%)=(before 1-(blank district medicine after disease index × treatment region medicine disease index)/(blank
Disease index before disease index after district's medicine × treatment region medicine)) × 100.
3.2.4 poisoning investigation method
Whether after dispenser, continuous 15d estimates medicament has poisoning to crop.
3.2.5 result of the test and analysis
Table 3 different disposal prevents and treats measurement result to cucumber bacterial angular leaf spot
Shown by above field experiment, tetramycin and Difenoconazole complex preparation obvious for the preventive effect of cucumber bacterial angular leaf spot
Be better than single dose, bactericidal effect increases progressively with the increase of dosage.According to field range estimation, within the scope of test dose, plant growth just
Often, occur without poisoning situation, significant for agricultural produce, be worthy to be popularized.
In sum, the present invention contains the bactericidal suspending agent of tetramycin and Difenoconazole, has good control efficiency to bitter rot or anthracnose of grape and cucumber bacterial angular leaf spot, and it is to Target crops safety.Complex preparation not only increases preventive effect, and expands the control to bacterium, so this compounded combination tool in production practices is of great significance.
Claims (8)
1. contain a bactericidal suspending agent for tetramycin and Difenoconazole, it is characterized in that: the active ingredient of this bactericidal suspending agent is tetramycin and Difenoconazole binary built, comprises following component by mass fraction: tetramycin 0.1 ~ 25 part; Difenoconazole 0.1 ~ 25 part; Dispersant 5 ~ 20 parts; Antifreezing agent 1 ~ 5 part; Thickener 0.1 ~ 2 part; Defoamer 0.1 ~ 0.8 part; Penetrating agent 0 ~ 10 part; PH value conditioning agent 0.1 ~ 5 part; Water, surplus.
2. a kind of bactericidal suspending agent containing tetramycin and Difenoconazole according to claim 1, is characterized in that: the mass ratio of tetramycin and Difenoconazole is 1 ~ 10: 50 ~ 1.
3. a kind of bactericidal suspending agent containing tetramycin and Difenoconazole according to claim 1, is characterized in that: the mass ratio of tetramycin and Difenoconazole is 1:10 ~ 10:1.
4. a kind of bactericidal suspending agent containing tetramycin and Difenoconazole according to claim 1, it is characterized in that: it is LG-3, GY-D1252, GY-D1256, SNWGF-01 that dispersant is selected from polycarboxylate code name, lignosulfonates code name is 201107,201108, alkylphenol polyoxyethylene methyl ether condensate sulfate, alkylsulfonate calcium salt, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, alkylphenol polyoxyethylene, polyoxyethylene carboxylate, aliphatic amine polyoxyethylene ether, one or more in fatty acid glyceride APEO.
5. a kind of bactericidal suspending agent containing tetramycin and Difenoconazole according to claim 1, is characterized in that: described antifreezing agent is selected from ethylene glycol, propane diols, one or more in glycerine.
6. a kind of bactericidal suspending agent containing tetramycin and Difenoconazole according to claim 1, is characterized in that: described thickener is selected from xanthans, carboxymethyl cellulose, carboxyethyl cellulose, methylcellulose, Magnesiumaluminumsilicate, in polyvinyl alcohol one or more.
7. a kind of bactericidal suspending agent containing tetramycin and Difenoconazole according to claim 1, is characterized in that: described defoamer is selected from silicone oil, silicone compound, C10-20 saturated fat acid compounds, one or more of C8-10 fatty alcohol.
8. prepare the preparation method of the bactericidal suspending agent described in claim 1 ~ 7 any one claim for one kind, it is characterized in that: first other auxiliary agents are mixed, mix through high speed shear, add active ingredient tetramycin and Difenoconazole, grind 2 ~ 3 hours in sand mill, make a diameter all at below 5mm, namely can be made into the aqueous suspension formulations of the present composition.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105191956A (en) * | 2015-09-15 | 2015-12-30 | 广东中迅农科股份有限公司 | Pesticide composition containing tetramycin and epoxiconazole |
| CN105851024A (en) * | 2016-05-21 | 2016-08-17 | 泉州绿邦贸易有限公司 | Bactericide composition and application thereof |
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| CN101379966A (en) * | 2008-08-06 | 2009-03-11 | 张少武 | Sterilization suspension agent containing difenoconazole |
| CN101856017A (en) * | 2010-05-17 | 2010-10-13 | 天津师范大学 | Suspension concentrate containing difenoconazole and preparation method thereof |
| CN103583532A (en) * | 2013-11-26 | 2014-02-19 | 辽宁微科生物工程有限公司 | Tetramycin complex and application |
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2015
- 2015-04-24 CN CN201510200281.XA patent/CN104798793A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101379966A (en) * | 2008-08-06 | 2009-03-11 | 张少武 | Sterilization suspension agent containing difenoconazole |
| CN101856017A (en) * | 2010-05-17 | 2010-10-13 | 天津师范大学 | Suspension concentrate containing difenoconazole and preparation method thereof |
| CN103583532A (en) * | 2013-11-26 | 2014-02-19 | 辽宁微科生物工程有限公司 | Tetramycin complex and application |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105191956A (en) * | 2015-09-15 | 2015-12-30 | 广东中迅农科股份有限公司 | Pesticide composition containing tetramycin and epoxiconazole |
| CN105851024A (en) * | 2016-05-21 | 2016-08-17 | 泉州绿邦贸易有限公司 | Bactericide composition and application thereof |
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