CN109350570B - Cosmetic preservative based on natural plant components and application thereof - Google Patents
Cosmetic preservative based on natural plant components and application thereof Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
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Abstract
The invention discloses a cosmetic preservative based on natural plant components and application thereof, wherein the preservative is prepared by compounding the following raw materials in parts by mass: 20-30 parts of litsea cubeba essential oil, 5-15 parts of oldenlandia diffusa extract and 60-70 parts of dihydric alcohol mixed solution. The dihydric alcohol mixed solution is formed by mixing 1, 2-pentanediol and 1, 2-hexanediol according to a mass ratio of 2-4: 1. The composite preservative disclosed by the invention is prepared by compounding the mixed dihydric alcohol extract of the oldenlandia diffusa, the litsea cubeba essential oil and the mixed liquid of the dihydric alcohol, has high safety, broad-spectrum antibacterial property and excellent preservative effect, replaces the traditional chemically synthesized preservative, reduces the potential safety risk of the chemically synthesized preservative, and has a great application value and a great market prospect.
Description
Technical Field
The invention relates to the technical field of cosmetics, in particular to a cosmetic preservative based on natural plant components and application thereof.
Background
The cosmetics are rich in nutrition and water, and are particularly suitable for growth and propagation of microorganisms, so that the cosmetics are extremely easy to be polluted by the microorganisms to cause putrefaction and deterioration in the processes of production, storage and use. Products polluted by microorganisms have potential safety hazards, can cause skin allergy, irritation and even infection, and bring harm to the health of consumers, so that the preservation is a very important link for quality control in the production of cosmetics, and the addition of preservatives is a commonly adopted and effective method.
At present, more than sixty preservatives are allowed to be used in the world, more than ten preservatives are commonly used, 51 preservatives approved in technical Specification for cosmetic safety (2015 edition) in China belong to chemically synthesized substances, most of the preservatives have certain irritation, sensitization and even biotoxicity, the safety of the preservatives is concerned all the time, and relevant regulations and standards strictly limit the use amount and the use range of the preservatives in cosmetics.
Research and development of mild, safe and efficient preservatives are always important research subjects in the field of cosmetics. Many plants have the functions of diminishing inflammation, resisting bacteria and preserving, and according to the existing anti-inflammatory and preservative practices of traditional Chinese medicines, natural, green, safe and efficient preservatives are searched from the plants, which is an important direction in the field of current cosmetic preservation.
Disclosure of Invention
The invention aims to provide a cosmetic preservative based on natural plant components, which has high safety, broad-spectrum antibiosis and good preservative effect.
In order to achieve the purpose, the invention provides a cosmetic preservative based on natural plant components, which is prepared by compounding the following raw materials in parts by mass: 20-30 parts of litsea cubeba essential oil, 5-15 parts of oldenlandia diffusa extract and 60-70 parts of dihydric alcohol mixed solution.
Preferably, the preservative is prepared by compounding the following raw materials in parts by mass: 22-28 parts of litsea cubeba essential oil, 7-12 parts of oldenlandia diffusa extract and 62-68 parts of dihydric alcohol mixed solution.
Optimally, the preservative is prepared by compounding the following raw materials in parts by mass: 26 parts of litsea cubeba essential oil, 10 parts of oldenlandia diffusa extract and 64 parts of dihydric alcohol mixed solution.
In a preferred embodiment, the diol mixture is prepared by mixing 1, 2-pentanediol and 1, 2-hexanediol in a mass ratio of 2-4: 1.
As a preferred embodiment, the litsea cubeba essential oil is prepared by the following method:
1) performing wall breaking treatment on the fresh litsea cubeba for 1-2 min to obtain pulp of the fresh litsea cubeba;
2) mixing the slurry obtained in the step 1) with distilled water according to a mass ratio of 1: 2-5, distilling for 2-3 h in an oil bath at a temperature of 120-130 ℃ by adopting a water vapor distillation method, and collecting an upper oil phase to obtain the litsea cubeba essential oil.
In a preferred embodiment, the extract of hedyotis diffusa is prepared by the following method:
1) cleaning, drying and crushing the whole plant of the oldenlandia diffusa, sieving the whole plant of the oldenlandia diffusa by using a 40-60-mesh sieve, and obtaining the powder of the oldenlandia diffusa at the undersize part;
2) mixing the oldenlandia diffusa powder obtained in the step 1) with a dihydric alcohol mixed solution according to a solid-liquid mass ratio of 1: 4-5, performing oscillation extraction for 1-2 hours at a temperature of 90-100 ℃, and filtering while hot by using a 100-200-mesh sieve to obtain a filtrate, wherein the dihydric alcohol mixed solution is prepared by mixing 1, 2-pentanediol and 1, 2-hexanediol according to a mass ratio of 2-4: 1;
3) cooling the filtrate obtained in the step 2) to room temperature, centrifuging at 4000-4500 rpm for 20-30 min, and decoloring the supernatant with activated carbon to obtain the oldenlandia diffusa extract.
The invention also provides an application of the cosmetic preservative based on the natural plant components, and the cosmetic preservative based on the natural plant components is added into cosmetics, and the mass usage amount of the cosmetic preservative is 1-3%.
The invention also provides an application of the cosmetic preservative based on the natural plant components in the facial cleanser, and the facial cleanser consists of the following raw materials in parts by mass: 10.0 to 14.0 parts of stearic acid, 12.0 to 16.0 parts of myristic acid, 3.0 to 7.0 parts of lauric acid, 2.0 to 4.0 parts of jojoba oil, 13.0 to 17.0 parts of sorbitol (70 mass percent), 8.0 to 13.0 parts of glycerol, 8.0 to 12.0 parts of 1, 3-butanediol, 1.0 to 3.0 parts of PEG-20 stearate, 3.0 to 6.0 parts of N-acyl-N-methyl sodium taurate, 0.15 to 0.3 part of EDTA disodium, 4.0 to 6.9 parts of potassium hydroxide (the total saponification rate is 70 to 80 percent), 2.0 to 3.0 parts of the natural plant component-based cosmetic preservative, and the balance of water, wherein the sum of the parts by mass of the raw materials is 100 parts.
As a preferred embodiment, the facial cleansing cream is composed of the following raw materials in parts by mass: 10.0 parts of stearic acid, 12.0 parts of myristic acid, 3.0 parts of lauric acid, 2.0 parts of jojoba oil, 13.0 parts of sorbitol (mass concentration is 70%), 13.0 parts of glycerol, 12.0 parts of 1, 3-butanediol, 3.0 parts of PEG-20 stearate, 6.0 parts of N-acyl-N-methyl sodium taurate, 0.3 part of disodium EDTA, 4.0 parts of potassium hydroxide (the saponification rate is 70%), 3.0 parts of the natural plant component-based cosmetic preservative, and 18.7 parts of water.
As a preferred embodiment, the facial cleansing cream is composed of the following raw materials in parts by mass: 14.0 parts of stearic acid, 16.0 parts of myristic acid, 7.0 parts of lauric acid, 4.0 parts of jojoba oil, 17.0 parts of sorbitol (mass concentration is 70%), 8.0 parts of glycerol, 8.0 parts of 1, 3-butanediol, 1.0 part of PEG-20 stearate, 3.0 parts of N-acyl-N-methyl sodium taurate, 0.15 part of EDTA disodium, 6.9 parts of potassium hydroxide (the total saponification rate reaches 80%), 2.0 parts of the natural plant component-based cosmetic preservative and 12.95 parts of water.
The invention also provides an application of the cosmetic preservative based on natural plant components in the skin cream, wherein the skin cream is prepared from the following raw materials in parts by mass: 2.0-6.0 parts of rice bran oil, 2.0-4.0 parts of jojoba oil, 1.0-2.0 parts of cetyl alcohol, 0.8-1.5 parts of glycerin monostearate, 4.0-7.0 parts of glycerol, 4.0-7.0 parts of 1, 2-propylene glycol, 1.0-1.5 parts of sodium stearate, 0.4-1.0 part of cetearyl pentosan glycoside, 1.0-3.0 parts of the cosmetic preservative based on natural plant components, and the balance of water, wherein the sum of the parts by mass of the raw materials is 100 parts.
As a preferred embodiment, the skin cream is composed of the following raw materials in parts by mass: rice bran oil 6.0 parts, jojoba oil 2.0 parts, cetyl alcohol 2.0 parts, glyceryl monostearate 0.8 parts, glycerin 7.0 parts, 1, 2-propylene glycol 4.0 parts, sodium stearate 1.5 parts, cetearyl pentosan 0.4 parts, and the above cosmetic preservatives based on natural plant components 3.0 parts, water 73.3 parts.
As a preferred embodiment, the skin cream is composed of the following raw materials in parts by mass: rice bran oil 2.0 parts, jojoba oil 4.0 parts, cetyl alcohol 1.0 parts, glyceryl monostearate 1.5 parts, glycerin 4.0 parts, 1, 2-propylene glycol 7.0 parts, sodium stearate 1.0 parts, cetearyl pentoside 1.0 parts, and the above cosmetic preservative based on natural plant components 1.0 parts, water 77.5 parts.
Compared with the prior art, the invention has the following advantages:
the litsea cubeba essential oil is volatile oil with lemon fragrance obtained by distilling litsea cubeba fruits, the main component of the litsea cubeba essential oil is citral, the content of the citral reaches 70-80 percent, and the litsea cubeba essential oil also contains limonene, methyl heptenone, linalool, geraniol, pinene and the like, the litsea cubeba essential oil has obvious inhibiting and killing effects on microorganisms such as aspergillus flavus, aspergillus niger, penicillium citrinum, rhizopus nigricans, rhizopus oryzae, mucor racemosus, staphylococcus aureus, bacillus subtilis, escherichia coli and the like, and the litsea cubeba essential oil is extremely safe for human bodies, is food spice and medical raw material which is allowed to be used, and is also daily seasoning oil which is deeply loved by common people; the hedyotis diffusa is rich in flavonoids which are bioactive substances beneficial to a human body and have antibacterial and bactericidal effects, the litsea cubeba essential oil and the hedyotis diffusa extract are matched and synergized to improve the antibacterial effect, and the hedyotis diffusa essential oil and the hedyotis diffusa extract have a good antiseptic effect on staphylococcus aureus, escherichia coli, candida albicans, pseudomonas aeruginosa and the like, and particularly have the most obvious effect on the staphylococcus aureus.
Secondly, the 1, 2-pentanediol and the 1, 2-hexanediol adopted by the invention have excellent moisturizing effect, are used as safe and mild moisturizers in a large amount in cosmetics, and simultaneously have broad-spectrum antibacterial effect on bacteria and fungi. According to the invention, 1, 2-pentanediol and 1, 2-hexanediol are compounded with litsea cubeba essential oil and oldenlandia diffusa extract for use, so that the synergistic effect is achieved, and the antibacterial effect is obvious.
Thirdly, the 1, 2-pentanediol and the 1, 2-hexanediol which are adopted in the invention are not only a humectant with an antiseptic effect, but also a good solvent, and the invention adopts the mixed solution of the 1, 2-pentanediol and the 1, 2-hexanediol as an extraction solvent to extract the active ingredients of the oldenlandia diffusa, thereby omitting the subsequent solvent removal process and simplifying the extraction process.
Fourthly, the compound preservative which is high in safety, broad-spectrum antibacterial and excellent in preservative effect is prepared by compounding the oldenlandia diffusa extract with the litsea cubeba essential oil, replaces the traditional chemical synthetic preservative, reduces the potential safety risk of the traditional chemical synthetic preservative, accords with the current healthy and green concept of cosmetics, and has a great application value and market prospect.
Detailed Description
The present invention will be described in further detail with reference to specific examples.
Example 1: the cosmetic preservative based on natural plant components is prepared by compounding the following raw materials in parts by mass: 20 parts of litsea cubeba essential oil, 15 parts of oldenlandia diffusa extract and 65 parts of dihydric alcohol mixed solution; wherein the dihydric alcohol mixed solution is formed by mixing 1, 2-pentanediol and 1, 2-hexanediol according to the mass ratio of 2: 1.
The litsea cubeba essential oil is prepared by the following method: performing wall breaking treatment on the fresh litsea cubeba fruits for 2min by using a wall breaking machine to obtain pulp of the fresh litsea cubeba fruits; mixing the obtained slurry with distilled water according to the mass ratio of 1:5, distilling for 3h in an oil bath at the temperature of 130 ℃ by adopting a steam distillation method, and collecting an upper oil phase to obtain the litsea cubeba essential oil.
The preparation method of the oldenlandia diffusa extract comprises the following steps: cleaning, drying and crushing the whole plant of the oldenlandia diffusa, sieving the plant by a 60-mesh sieve to obtain the powder of the oldenlandia diffusa at the undersize part, and mixing the powder according to the weight ratio of 1:5, adding a dihydric alcohol mixed solution into the solid-liquid mass ratio, oscillating and extracting for 2h at 100 ℃, filtering while hot by using a 200-mesh sieve, cooling the filtrate to room temperature, centrifuging for 30min at 4500rpm, and decoloring the supernatant by using activated carbon to obtain the oldenlandia diffusa extract. Wherein the dihydric alcohol mixed solution is prepared from 1, 2-pentanediol and 1, 2-hexanediol according to a mass ratio of 2:1 are mixed.
Example 2: the cosmetic preservative based on natural plant components is prepared by compounding the following raw materials in parts by mass: 26 parts of litsea cubeba essential oil, 10 parts of oldenlandia diffusa extract and 64 parts of dihydric alcohol mixed solution. Wherein the dihydric alcohol mixed solution is formed by mixing 1, 2-pentanediol and 1, 2-hexanediol according to the mass ratio of 2.5: 1.
The litsea cubeba essential oil is prepared by the following method: performing wall breaking treatment on the fresh litsea cubeba fruits for 2min by using a wall breaking machine to obtain pulp of the fresh litsea cubeba fruits; mixing the obtained slurry with distilled water according to the mass ratio of 1:2, distilling for 2h in an oil bath at the temperature of 120 ℃ by adopting a steam distillation method, and collecting an upper oil phase to obtain the litsea cubeba essential oil.
The preparation method of the oldenlandia diffusa extract comprises the following steps: cleaning, drying and crushing the whole plant of the oldenlandia diffusa, sieving the plant by a 40-mesh sieve to obtain the powder of the oldenlandia diffusa at the sieved part, and mixing the powder of the oldenlandia diffusa with the powder of 1:4, adding the dihydric alcohol mixed solution into the solid-liquid mass ratio, oscillating and extracting for 2h at 90 ℃, filtering while hot by using a 100-mesh sieve, cooling the filtrate to room temperature, centrifuging for 30min at 4000rpm, and decoloring the supernatant by using activated carbon to obtain the oldenlandia diffusa extract. Wherein the dihydric alcohol mixed solution is prepared from 1, 2-pentanediol and 1, 2-hexanediol according to a mass ratio of 2:1 are mixed.
Example 3: the cosmetic preservative based on natural plant components is prepared by compounding the following raw materials in parts by mass: 25 parts of litsea cubeba essential oil, 10 parts of oldenlandia diffusa extract and 65 parts of dihydric alcohol mixed solution. Wherein the dihydric alcohol mixed solution is formed by mixing 1, 2-pentanediol and 1, 2-hexanediol according to the mass ratio of 3.5: 1.
The litsea cubeba essential oil is prepared by the following method: breaking the wall of the fresh litsea cubeba fruits by a wall breaking machine for 1.5min to obtain pulp of the fresh litsea cubeba fruits; mixing the obtained slurry with distilled water according to the mass ratio of 1:4.5, distilling for 2h in an oil bath at the temperature of 125 ℃ by adopting a steam distillation method, and collecting an upper oil phase to obtain the litsea cubeba essential oil.
The preparation method of the oldenlandia diffusa extract comprises the following steps: cleaning, drying and crushing the whole plant of the oldenlandia diffusa, sieving the plant by a 60-mesh sieve to obtain the powder of the oldenlandia diffusa at the undersize part, and mixing the powder according to the weight ratio of 1:4.5, adding the dihydric alcohol mixed solution at the solid-liquid mass ratio, oscillating and extracting for 2h at 100 ℃, filtering while hot by using a 150-mesh sieve, cooling the filtrate, centrifuging at 4500rpm for 20min, and decoloring the supernatant by using activated carbon to obtain the oldenlandia diffusa extract. Wherein the dihydric alcohol mixed solution is prepared from 1, 2-pentanediol and 1, 2-hexanediol according to a mass ratio of 3:1 are mixed.
Example 4: the cosmetic preservative based on natural plant components is prepared by compounding the following raw materials in parts by mass: 22 parts of litsea cubeba essential oil, 10 parts of oldenlandia diffusa extract and 68 parts of dihydric alcohol mixed solution. Wherein the dihydric alcohol mixed solution is formed by mixing 1, 2-pentanediol and 1, 2-hexanediol according to the mass ratio of 3: 1.
The litsea cubeba essential oil is prepared by the following method: performing wall breaking treatment on the fresh litsea cubeba fruits for 1min by using a wall breaking machine to obtain pulp of the fresh litsea cubeba fruits; mixing the obtained slurry with distilled water according to the mass ratio of 1:4, distilling for 2.5h in an oil bath at the temperature of 125 ℃ by adopting a steam distillation method, and collecting an upper oil phase to obtain the litsea cubeba essential oil.
The preparation method of the oldenlandia diffusa extract comprises the following steps: cleaning, drying and crushing the whole plant of the oldenlandia diffusa, sieving the plant by a 40-mesh sieve to obtain the powder of the oldenlandia diffusa at the sieved part, and mixing the powder of the oldenlandia diffusa with the powder of 1:4, adding a dihydric alcohol mixed solution into the solid-liquid mass ratio, oscillating and extracting for 1h at 95 ℃, filtering while hot by using a 150-mesh sieve, cooling the filtrate, centrifuging for 30min at 4200rpm, and decoloring the supernatant by using activated carbon to obtain the oldenlandia diffusa extract. Wherein the dihydric alcohol mixed solution is prepared from 1, 2-pentanediol and 1, 2-hexanediol according to a mass ratio of 4:1 are mixed.
Example 5: the cosmetic preservative based on natural plant components is prepared by compounding the following raw materials in parts by mass: 30 parts of litsea cubeba essential oil, 10 parts of oldenlandia diffusa extract and 60 parts of dihydric alcohol mixed solution. Wherein the dihydric alcohol mixed solution is formed by mixing 1, 2-pentanediol and 1, 2-hexanediol according to the mass ratio of 4: 1.
The litsea cubeba essential oil is prepared by the following method: performing wall breaking treatment on the fresh litsea cubeba fruits for 2min by using a wall breaking machine to obtain pulp of the fresh litsea cubeba fruits; mixing the obtained slurry with distilled water according to the mass ratio of 1:4, distilling for 3h in an oil bath at the temperature of 120 ℃ by adopting a steam distillation method, and collecting an upper oil phase to obtain the litsea cubeba essential oil.
The preparation method of the oldenlandia diffusa extract comprises the following steps: cleaning, drying and crushing the whole plant of the oldenlandia diffusa, sieving the plant by a 40-mesh sieve to obtain the powder of the oldenlandia diffusa at the sieved part, and mixing the powder of the oldenlandia diffusa with the powder of 1:4, adding a dihydric alcohol mixed solution into the solid-liquid mass ratio, oscillating and extracting for 1h at 100 ℃, filtering while hot by using a 200-mesh sieve, cooling the filtrate, centrifuging for 30min at 4000rpm, and decoloring the supernatant by using activated carbon to obtain the oldenlandia diffusa extract. Wherein the dihydric alcohol mixed solution is prepared from 1, 2-pentanediol and 1, 2-hexanediol according to a mass ratio of 2:1 are mixed.
Effect example 1: bacteriostasis test
1. Material
1.1 test materials: examples 1 to 4 the obtained preservatives.
1.2 test strains:
bacteria: staphylococcus aureus, escherichia coli and pseudomonas aeruginosa.
Mold and yeast: saccharomyces cerevisiae and Aspergillus niger.
1.3 test medium:
bacteria culture medium: nutrient agar culture medium, and autoclaving at 121 deg.C for 30 min.
And (3) yeast culture medium: YPD medium, autoclaved at 115 deg.C for 30 min.
And (3) a mildew culture medium: sterilizing the Chao's culture medium at 115 deg.C under high pressure for 30 min.
2. Experimental methods
2.1 preparation of the bacterial suspension
Before the experiment, each strain is inoculated to a corresponding culture medium slant. Culturing bacteria (Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa) in constant temperature incubator at 36 + -1 deg.C for 36-48 hr, respectively selecting appropriate amount of bacterial colony, mixing with sterile normal saline, and making into bacterial suspension with concentration of about 1.0 × 108CFU/ml, stored at 4 ℃ for further use. The mold is cultured in a constant temperature incubator at 27 +/-1 ℃ for 120 hours. The yeast is cultured in a constant temperature incubator at 27 +/-1 ℃ for 24 hours. Respectively selecting appropriate amount of fungus and yeast colonies, mixing in sterile physiological saline to obtain fungus suspension with concentration of about 1.0 × 106CFU/ml, concentration of yeast spore suspension is about 1.0 × 108CFU/ml, stored at 4 ℃ for further use.
2.2 the bacteriostasis test is carried out by adopting a filter paper diffusion method under the aseptic condition in the whole process. The filter paper is punched into a circular filter paper sheet with the diameter of 6mm by a puncher, and the circular filter paper sheet is sterilized and dried at 121 ℃ for standby. And (3) respectively soaking the filter paper sheets in the preservatives of the groups 1-4 in the examples for 20min, respectively pouring the sterilized prepared solid culture medium into culture dishes, and sucking 0.2ml of bacterial suspension to uniformly coat on a flat plate after cooling and solidification. Respectively clamping filter paper sheets soaked in the preservative by using sterile tweezers, attaching the filter paper sheets to a bacteria-containing flat plate, taking the filter paper sheets soaked in sterile water as a blank control, placing a bacteria culture dish in a constant-temperature incubator at 37 ℃ for flat plate inverted culture for 24 hours, placing a mould and yeast culture dish in a constant-temperature incubator at 28 ℃ for flat plate inverted culture for 72-120 hours, measuring the diameter of a bacteriostatic ring, setting 3 times of repetition, and taking the average value.
3. Results and analysis
Table 1 shows the results of the experiment of the bacteriostatic effect of the preservatives of examples 1 to 4.
TABLE 1 results of the experiment of the bacteriostatic effect
From table 1, it can be seen that the preservatives of 4 groups in examples 1 to 4 all have antiseptic and bacteriostatic effects on five microorganisms, the diameters of inhibition zones are all larger than 10mm, the effects are obvious, and the preservatives have broad-spectrum bacteriostatic properties, wherein the preservative prepared in example 2 has the best effect. In summary, the groups of preservatives in examples 1-4 have the following bacteriostatic ability on five bacteria: escherichia coli, pseudomonas aeruginosa, saccharomycetes, staphylococcus aureus and aspergillus niger.
Effect example 2: corrosion challenge test
The 4 groups of preservatives of examples 1-4 were applied to a cleansing cream, and 2% of the preservatives of examples 1-4 were added to the system, respectively, to perform a preservative challenge test.
1. Material
1.1 test materials
4 groups of facial cleansers to which the preservatives prepared in examples 1 to 4 were added.
1.2 test strains
Bacteria: staphylococcus aureus, escherichia coli and pseudomonas aeruginosa.
Mold and yeast: saccharomyces cerevisiae and Aspergillus niger.
1.3 test Medium
Bacteria culture medium: nutrient agar culture medium, and autoclaving at 121 deg.C for 30 min.
And (3) yeast culture medium: YPD medium, autoclaved at 115 deg.C for 30 min.
And (3) a mildew culture medium: sterilizing the Chao's culture medium at 115 deg.C under high pressure for 30 min.
2. Experimental methods
2.1 preparation of the bacterial suspension
Before the experiment, each strain is inoculated to a corresponding culture medium slant. Culturing bacteria (Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa) in constant temperature incubator at 36 + -1 deg.C for 36-48 hr, respectively selecting appropriate amount of bacterial colony, mixing with sterile normal saline, and making into mixed bacterial suspension with a certain concentration of about 1.0 × 108CFU/ml, stored at 4 ℃ for further use. Culturing mould in a constant temperature incubator at 27 + -1 deg.C for 120 hr, culturing yeast in a constant temperature incubator at 27 + -1 deg.C for 24 hr, respectively selecting appropriate amount of mould and yeast colonyMixing with sterile normal saline to obtain mixed fungal suspension with a concentration of about 1.0 × 107CFU/ml, stored at 4 ℃ for further use.
2.2 Corrosion challenge test
A one-time bacterial-addition 28-day microbial challenge test was used, which refers to the method for testing preservative efficacy in the United states Pharmacopeia microbial challenge test. Respectively weighing 4 groups of facial cleanser samples 100g as test samples, filling the test samples into sample bottles, and respectively adding a proper amount of mixed bacterial suspension to ensure that each gram of sample finally contains 10 bacteria6CFU/g, the amount of the mold yeast is 105CFU/g, mixing well, and culturing in constant temperature incubator at 36 + -1 deg.C and 27 + -1 deg.C respectively. Samples were taken on day 0, day 3, day 7, day 14, day 21 and day 28 of inoculation for bacterial load analysis.
3. Evaluation criteria
Initial inoculation amount: bacterium 106CFU/g～107CFU/g (mL), fungus 105CFU/g～106CFU/g(mL)：
(28 th day), the sample contains bacteria or fungi > 103CFU/g (ml), which fails challenge tests with microbial attack, indicating that the preservative system of the sample does not effectively act to inhibit microorganisms and that the product is susceptible to microbial contamination during manufacture, storage and use.
② on day 28, the sample contains bacteria or fungi at 102CFU/g～103CFU/g (mL), the sample conditionally passes challenge tests, namely when the protein or other animal and plant material components in the product are not particularly high, the production sanitary environment meets the requirements, and the package is not easy to generate secondary pollution, the mildew-proof system can be used, otherwise, the mildew-proof system cannot be used.
③ at day 28, the sample contains bacteria or fungi at 10 CFU/g-102CFU/g (mL) shows that the preservative system of the sample has strong inhibiting and killing effects on microorganisms, and the product is not easily polluted by the microorganisms during production, storage and use through challenge tests.
And from day 7, the bacteria or fungi in the sample is less than 10CFU/g (mL), which indicates that the preservative system of the sample has extremely strong inhibiting and killing effects on microorganisms, and the product is not easily polluted by the microorganisms during production, storage and use through challenge tests.
3. Results and analysis
Table 2 below shows the results of the preservative challenge experiments for 4 groups of facial cleansers with preservatives prepared in examples 1-4.
Table 2 corrosion protection challenge experimental results
Table 2 the results show that: on the 28 th day, the contents of bacteria, mold and yeast in all samples are less than 10CFU/g (mL), which shows that the preservatives in the groups of examples 1-4 have strong inhibiting and killing effects on several microorganisms, and through challenge tests, the product is not easily polluted by the microorganisms during production, storage and use. The four preservatives of embodiments 1-4 can all be used as an antiseptic system of the cleansing cream to replace the traditional chemically synthesized preservative, and provide safer and milder products for consumers.
Application example 1 and application example 2:
the cosmetic preservative based on natural plant components prepared in example 1 is applied to a facial cleanser, which comprises the following raw materials in parts by mass, as shown in table 3.
TABLE 3 cleansing cream formulation examples
Application example 3 and application example 4:
the natural plant component-based cosmetic preservative prepared in example 2 is applied to skin cream, and the skin cream comprises the following raw materials in parts by mass, and is specifically shown in table 4.
Table 4 skin cream formulation examples
| Raw materials | Application example 3 (parts by mass) | Application example 4 (parts by mass) |
| Rice bran oil | 6.0 | 2.0 |
| Jojoba oil | 2.0 | 4.0 |
| Cetyl alcohol | 2.0 | 1.0 |
| Glyceryl monostearate | 0.8 | 1.5 |
| Glycerol | 7.0 | 4.0 |
| 1, 2-propanediol | 4.0 | 7.0 |
| Sodium stearate | 1.5 | 1.0 |
| Cetearyl alcohol pentoside | 0.4 | 1.0 |
| Preservative prepared in example 2 | 3.0 | 1.0 |
| Water (W) | 73.3 | 77.5 |
Effect example 3: quality detection
Table 5 shows the results of microbiological indicators of the products obtained in application examples 1 to 4.
TABLE 5 application examples 1-4 microbiological indicator test results
The detection method of the microorganism indexes is carried out according to technical Specification for cosmetic safety (2015 edition). The microbial indexes of the cleansing cream products prepared in the application examples 1-2 and the skin cream products prepared in the application examples 3-4 meet the microbial index requirements of technical standards for cosmetic safety.
The foregoing is only an embodiment of the present invention, and it should be noted that the remaining portions not described in detail are prior art, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the protection scope of the present invention.
Claims (5)
1. The cosmetic preservative based on natural plant components is characterized by being prepared by compounding the following raw materials in parts by mass: 22-28 parts of litsea cubeba essential oil, 7-12 parts of oldenlandia diffusa extract and 62-68 parts of dihydric alcohol mixed solution;
the dihydric alcohol mixed solution is formed by mixing 1, 2-pentanediol and 1, 2-hexanediol according to a mass ratio of 2-4: 1;
the litsea cubeba essential oil is prepared by the following method:
1) performing wall breaking treatment on the fresh litsea cubeba for 1-2 min to obtain pulp of the fresh litsea cubeba;
2) mixing the slurry obtained in the step 1) with distilled water according to a mass ratio of 1: 2-5, distilling for 2-3 h in an oil bath at a temperature of 120-130 ℃ by adopting a water vapor distillation method, and collecting an upper oil phase to obtain litsea cubeba essential oil;
the oldenlandia diffusa extracting solution is prepared by the following method:
1) cleaning, drying and crushing the whole spreading hedyotis herb, and sieving the spreading hedyotis herb by using a 40-60-mesh sieve to obtain spreading hedyotis herb powder on the undersize part;
2) mixing the oldenlandia diffusa powder obtained in the step 1) with the dihydric alcohol mixed solution according to a solid-liquid mass ratio of 1: 4-5, performing oscillation extraction for 1-2 hours at a temperature of 90-100 ℃, and filtering while hot by using a 100-200-mesh sieve to obtain a filtrate;
3) cooling the filtrate obtained in the step 2) to room temperature, centrifuging at 4000-4500 rpm for 20-30 min, and decoloring the supernatant with activated carbon to obtain the oldenlandia diffusa extract.
2. The natural plant component-based cosmetic preservative according to claim 1, which is prepared by compounding the following raw materials in parts by mass: 25-26 parts of litsea cubeba essential oil, 10-12 parts of oldenlandia diffusa extract and 64-65 parts of dihydric alcohol mixed solution.
3. The non-therapeutic use of the natural plant component-based cosmetic preservative according to claim 1 or 2, wherein the natural plant component-based cosmetic preservative is added to a cosmetic in an amount of 1 to 3% by mass.
4. The use of the cosmetic preservative based on natural plant components according to claim 1 for the preparation of a facial cleanser, wherein the facial cleanser consists of the following raw materials in parts by mass: 10.0-14.0 parts of stearic acid, 12.0-16.0 parts of myristic acid, 3.0-7.0 parts of lauric acid, 2.0-4.0 parts of jojoba oil, 13.0-17.0 parts of sorbitol, 8.0-13.0 parts of glycerol, 8.0-12.0 parts of 1, 3-butanediol, 1.0-3.0 parts of PEG-20 stearate, 3.0-6.0 parts of N-acyl-N-methyl sodium taurate, 0.15-0.3 part of EDTA disodium, 4.0-6.9 parts of potassium hydroxide, 2.0-3.0 parts of the natural plant component-based cosmetic preservative as claimed in claim 1 or 2, and the balance of water, wherein the sum of the parts by mass of the raw materials is 100.
5. The use of the cosmetic preservatives based on natural plant components in skin cream according to claim 1, characterized in that the skin cream is composed of the following raw materials in parts by mass: 2.0-6.0 parts of rice bran oil, 2.0-4.0 parts of jojoba oil, 1.0-2.0 parts of cetyl alcohol, 0.8-1.5 parts of glycerin monostearate, 4.0-7.0 parts of glycerol, 4.0-7.0 parts of 1, 2-propylene glycol, 1.0-1.5 parts of sodium stearate, 0.4-1.0 part of cetearyl pentosan glycoside, 1.0-3.0 parts of the natural plant component-based cosmetic preservative as claimed in claim 1 or 2, and the balance of water, wherein the sum of the parts by weight of the raw materials is 100 parts.
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| CN108676622A (en) * | 2018-06-26 | 2018-10-19 | 清远市瑶康生物科技有限公司 | A kind of preparation method of the litsea cubeba oil with antibacterial activity |
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| CN103622871A (en) * | 2013-11-29 | 2014-03-12 | 上海相宜本草化妆品股份有限公司 | Anticorrosive composition containing polyhydric alcohols and Chinese herbal medicines and application of anticorrosive composition in cosmetics |
| CN108676622A (en) * | 2018-06-26 | 2018-10-19 | 清远市瑶康生物科技有限公司 | A kind of preparation method of the litsea cubeba oil with antibacterial activity |
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