CN113827523A - Rose composition and application thereof - Google Patents
Rose composition and application thereof Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
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- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
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Abstract
The invention discloses a rose composition, which is prepared from rose fermentation product and plant extract according to the weight ratio of 1: 0.05-2, and the rose leavening is obtained by fermenting and extracting the rose raw material with ganoderma lucidum strain (Latin article name of Ganodermalucidum). The rose fermentation product is mixed with other commercially available sandalwood hydrolat, sandalwood essential oil, jasmine essential oil and the like to obtain the rose composition, and the rose composition has a synergistic effect in the aspects of anti-aging and whitening activities when applied to cosmetics.
Description
Technical Field
The invention belongs to the technical field of fermentation and functional cosmetics, and particularly relates to a rose composition and application thereof.
Background
The rose flower tea is drunk as a medicinal and edible traditional Chinese medicinal material, and fresh rose flowers or dried flower tea has unique functions of clearing away heat and toxic materials, relaxing bowel, regulating menstruation, activating blood circulation, promoting metabolism and the like, and the rose flowers are also applied to clinical medicines and partial health care products. The Rose with Chinese ink (Rose chinese janensis Jacq "crimson Glory" H.T.) belongs to the deciduous shrub of Rose (Rosa) in Rosaceae (Rosaceae), the origin is china, the cultivation history is long, and the Rose with ink has the functions of food and medicine besides the ornamental and cultural value. The rose black extract has the main effects of resisting oxidation, whitening skin, removing freckles, promoting skin permeation, eliminating free radicals, and helping other nutrient components to be absorbed by the skin; has certain tyrosinase inhibiting effect, and can be used for whitening skin. Because of its fragrant smell, it is commonly used in cosmetics for the formulation of perfumes and fragrances.
The rose has various active substances, and contains vitamins, proteins, amino acids, volatile oil, pigments, polyphenol, flavone, trace elements and the like. At present, the extraction of effective components in roses mainly adopts physical and chemical extraction means such as an alcohol reflux extraction technology, an ultrasonic extraction technology and a desorption-thermal extraction two-step method, and the extraction of active components by utilizing a microbial fermentation technology is less reported.
The rose petals contain cellulose, pectin and other components, the existing commonly used lactic acid bacteria, saccharomycetes and acetic acid bacteria rarely produce cellulase and pectinase, 0.01-0.03% of pectinase and cellulase are used for crushing before fermentation, the purity of commercialized enzyme cannot reach 100%, impurities can be doped into a fermentation system, the requirement on the purity of cosmetic raw materials is not met, the rose petals are difficult to perfectly compound with commonly used plant extracts, and the rose petals can play a role as a whitening component in cosmetics.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a rose composition and application thereof, wherein the rose composition capable of being compounded with plant extracts is lacked, and the defects and shortcomings in the background art are overcome.
In order to solve the technical problems, the technical scheme provided by the invention is as follows:
a rose composition comprising a rose ferment and a plant extract in a ratio of 1: 0.05-2, and the rose leavening is obtained by fermenting and extracting a rose raw material with a Ganoderma lucidum strain (Latin under the name of Ganoderma lucidum).
The rose composition is prepared by fermenting rose raw materials with Ganoderma lucidum strains (Latin, named as Ganoderma lucidum) and extracting rose fermentation products, is mixed with other commercially available essential oil and hydrolat, and has synergistic effect in the aspects of anti-aging and whitening activity.
Preferably, the plant extract comprises essential oil and/or hydrolat, the essential oil comprises plant alcohol substances, and the hydrolat is distillation stock solution separated in the process of refining the essential oil.
Preferably, the essential oil, hydrolat and plant flower, stem and leaf water extract comprise one or more of sandalwood hydrolat, sandalwood essential oil, jasmine hydrolat, rose essential oil and rose hydrolat.
The rose fermentation product is compounded with rose essential oil, jasmine essential oil and sandalwood essential oil, and the compounding mass ratio is as follows: rose fermentation product: rose essential oil: and (3) jasmine essential oil: sandalwood essential oil 1: 0.05-0.1: 0.05-0.1: 0.05-0.1; more preferably, the rose fermentation: rose essential oil jasmine essential oil: sandalwood essential oil 1: 0.05: 0.05: 0.05.
preferably, the ganoderma lucidum strain is derived from China general microbiological culture collection center, and the preservation number is CGMCC 5.1817.
Preferably, the preparation method of the rose fermentation product comprises the following steps:
(1) the activated ganoderma lucidum strain is preserved by China general microbiological culture Collection center with the preservation number of CGMCC 5.1817;
(2) fermentation culture: sterilizing dried flos Rosae Rugosae, inoculating activated Ganoderma strain, fermenting and culturing for 7-14 days;
(3) extraction: extracting the product obtained in the step (2) according to the mass ratio of 1:20-40 to obtain the rose fermentation product.
The fermentation culture is carried out on the selected ganoderma lucidum strain and the rose, polyphenol and flavone in the rose, polysaccharide and triterpene in the ganoderma lucidum have good skin care effects, the fermentation culture of the ganoderma lucidum strain and the rose interact with each other, the obtained rose fermentation product DPPH free radical clearance rate (diluted by 200 times), superoxide anion clearance rate, hydroxyl free radical clearance rate, ABTS free radical clearance rate (diluted by 200 times), tyrosinase activity inhibition rate and total reducing power are high, and the rose fermentation product can be used as an anti-aging and whitening active ingredient to play an excellent effect.
Preferably, the specific method for activating the ganoderma lucidum strain in the step (1) comprises the following steps: culturing Ganoderma strain at 25-28 deg.C in MEA culture medium (preferably malt extract 1.2-2%), agar 1-2%, and glucose 1-2% for 5-7 days to activate strain.
The MEA culture medium is suitable for the growth of the ganoderma lucidum strain, the preferable components of the culture medium can culture fungi under the optimal conditions, and the cultured ganoderma lucidum strain is suitable for the fermentation of the dried rose flowers.
Preferably, the dried rose flowers in step (2) are black red rose dried flowers.
The ganoderma lucidum strain belongs to white rot fungi, and the secreted enzyme determines that the ganoderma lucidum strain can grow on flowers and is suitable for fermenting the dried black red rose flowers.
Preferably, the temperature of the sterilization treatment in the step (2) is 115 ℃ and 121 ℃, and the time is 4-6 h; the inoculation amount of the ganoderma lucidum strain is 5-10% of the mass of the dried rose flowers; the fermentation culture temperature is 25-28 ℃; the extraction in the step (3) is ethanol extraction, the concentration of the ethanol is 60-90%, the extraction temperature is 50-70 ℃, and the extraction time is 3-5 h.
Under the same technical concept, the application also provides the application of the rose composition, and the rose composition is applied as an active ingredient of cosmetics.
Compared with the prior art, the invention has the beneficial effects that:
(1) strains suitable for rose fermentation are screened, and the obtained rose fermentation product has better anti-aging and whitening activities;
(2) the rose fermentation product is mixed with other commercially available sandalwood hydrolat, sandalwood essential oil, jasmine essential oil and the like to obtain the rose composition, and the rose composition has a synergistic effect in the aspects of anti-aging and whitening activities when applied to cosmetics.
Detailed Description
In order to facilitate an understanding of the present invention, the present invention will be described more fully and in detail with reference to the preferred embodiments, but the scope of the present invention is not limited to the specific embodiments below.
Unless otherwise defined, all terms of art used hereinafter have the same meaning as commonly understood by one of ordinary skill in the art. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of the present invention.
Unless otherwise specified, various raw materials, reagents, instruments, equipment and the like used in the present invention may be commercially available or may be prepared by existing methods, and the species used in the examples of the present invention and comparative examples are commercially available biomaterials.
Example 1:
in this example, a rose is selected and a rose composition is prepared as follows:
1. activation of glossy ganoderma strain
Ganoderma lucidum, China general microbiological culture Collection center (CGMCC 5.1817), Latin with the name of Ganoderma lucidum, wherein the Ganoderma lucidum can be purchased in China general microbiological culture Collection center through retrieval (CGMCC 5.1817); culturing Ganoderma strain in MEA (malt extract 1.2%, agar 2%, and glucose 1%) culture medium at 28 deg.C for 7 days to activate strain;
2. fermentation of the fermented product of the black red rose:
placing 6g dried flos Rosae Rugosae in 9 x 9cm dish, sterilizing at 121 deg.C for 4 hr, inoculating activated Ganoderma with inoculum size of 5%, and culturing at 28 deg.C for 7-14 days;
3. alcohol extraction of the fermented product of the black red rose:
the fermented black rose is prepared according to the following steps of 1:20 percent of ethanol is extracted, the concentration of the ethanol is 60 percent, the extraction temperature is 70 ℃, and the extraction time is 3 hours.
After the preparation, the fermented product of the rose pink of the embodiment is obtained; mixing the fermented product with commercially available lignum Santali albi hydrosol, lignum Santali albi essential oil, flos Jasmini sambac hydrosol, oleum Rosae Rugosae and flos Rosae Rugosae hydrosol to obtain flos Tamarindi Indicae composition, wherein commercially available lignum Santali albi hydrosol and lignum Santali albi essential oil are from Oshadhi.
Extracting plant essential oil: in the experiment, the water vapor distillation method is adopted to extract part of the plant essential oil selected in the embodiment. Pulverizing decoction pieces of Chinese medicinal materials, sieving with 80 mesh sieve, soaking for 2 hr, adding at a material-liquid ratio of 1:20, heating and distilling for 4 hr to obtain distillate, and adding appropriate amount of NaCl to separate water layer from oil layer. Separating the oil layer, adding a small amount of anhydrous sodium sulfate, drying, and filtering to obtain plant essential oil.
The following contents are mass to volume ratios (g content per 100 ml)
Rose essential oil: the content of citronellol is 20-34%; the content of nerol is 5-12 percent, and the content of geraniol is 15-22 percent;
sandalwood essential oil: the content of Z-alpha-santalol is 20-30.2%, the content of trans-alpha-bergamotol is 5-12.1%, the content of epi-beta-santalol is 12-18.9%, and the content of Z-beta-santalol is 15-28.3%;
and (3) jasmine essential oil: linalool content of 15-33.5%, acetic acid cis-3-hexenyl ester content of 7.5-15.5%, juniper alcohol content of 3.8-7.6%, nerol content of 2-5.2%;
in this embodiment, the water extracts of the flowers, stems and leaves of the plants and the rubia sativa composition can be selected for compounding, and the following preparation methods of the water extracts of the flowers, stems and leaves of the plants are as follows:
the preparation method of the jasmine aqueous extract comprises the following steps: adding 20 ml of water into 1g of jasmine, extracting for 3 hours at 90 ℃, centrifuging at 9000 rpm, and taking supernatant;
the preparation method of the sandalwood aqueous extract comprises the following steps: adding 20 ml of water into 1g of sandalwood, extracting at 90 ℃ for 3 hours, centrifuging at 9000 rpm, and taking supernatant.
The performance test of the ink red rose composition of the embodiment is carried out by mixing the ink red rose fermentation product with the commercially available essential oil and the pure dew according to the following mixing ratio:
the fermented product of the rose: 1:1 of sandalwood hydrosol;
② fermented products of the black red rose: 1:1 of jasmine hydrosol;
③ fermentation product of black red rose: and (3) jasmine hydrosol: 1: 0.05;
fourthly, the fermented product of the black red rose: and (3) jasmine hydrosol: rose essential oil, jasmine essential oil 1: 0.05: 0.05;
fifthly, the fermented product of the black red rose: and (3) jasmine hydrosol: rose essential oil jasmine essential oil: 1: 0.05: 0.05: 0.05;
sixthly, the fermented product of the rose containing the black red: rose essential oil jasmine essential oil: sandalwood essential oil 1: 0.05: 0.05: 0.05.
comparative example 1:
the comparative example adopts lactic acid bacteria and saccharomycetes to ferment the rose black, and the specific preparation method is as follows:
the rose composition was prepared as follows:
1. fermentation strain activation
Yeast: the preservation number is CCTCC No: m20191046 Saccharomyces cerevisiae, cell, ZLG-6;
lactic acid bacteria: lactobacillus plantarum with a preservation number of CCTCC No: lactobacillus plantarum pich-2 of M20191045;
activation of yeast: inoculating the strain in YPD culture medium, culturing at 30 deg.C for 36-48h, and continuously passaging for 3 times to activate the strain sufficiently until viable count is above 1.0 × 107 CFU/mL;
activating lactic acid bacteria: inoculating the strain in MRS culture medium, culturing at 35 deg.C for 24 hr, and continuously passaging for 3 times to activate the strain sufficiently until viable count is more than 1.0 × 108 CFU/mL.
2. Fermentation of the fermented product of the black red rose:
crude enzyme liquid (pectinase and cellulase 1: 1): the ratio of the dry rose powder to the dry rose powder is 20:1, the enzyme activity of pectinase is as follows: 2500U/mL, cellulase activity: extracting at 50 deg.C for 3 hr at 3000U/mL, centrifuging at 9000r/min for 15min to obtain enzymatic hydrolysate, and collecting supernatant for index detection.
The enzymatic hydrolysate of the rose is respectively filled in 250mL reagent bottles with blue caps (200 mL/bottle), sterilized at 115 ℃ for 30min, inoculated with activated lactobacillus strain with the inoculation amount of 5 percent of the volume of the dried rose flower and cultured at 37 ℃ for 7 days; then yeast is inoculated according to the proportion of 1 percent of the volume of the dried rose flowers, and after fermentation for 7 days, the rose flowers are centrifuged at 9000r/min for 15min to obtain rose fermentation products for index detection.
3. Alcohol extraction of the fermented product of the black red rose:
the fermented black rose is prepared according to the following steps of 1:20 percent of ethanol is extracted, the concentration of the ethanol is 60 percent, the extraction temperature is 70 ℃, and the extraction time is 3 hours.
After the preparation was completed, the fermented product of the rubia rosea of this comparative example was obtained.
Compounding the fermented product of the rose black obtained in the comparative example 1 with commercially available essential oil and hydrolat, wherein the compounding ratio is as follows:
seventhly, fermenting the black red rose: and (3) jasmine hydrosol: rose essential oil jasmine essential oil: 1: 0.05: 0.05: 0.05.
comparative example 2:
the comparative example adopts aspergillus oryzae strain to ferment the rose malus asiatica, and the specific preparation method is as follows:
the rose composition was prepared as follows:
1. fermentation strain activation
Aspergillus oryzae strain is derived from China general microbiological culture Collection center (CGMCC 3.13905), and Aspergillus oryzae spore is inoculated into liquid culture medium (glucose 2%, peptone 1%, KH2PO40.1%, MgSO40.05% and NaCl 0.05%) at 28 deg.C, and cultured for 7 days for strain activation.
2. Fermentation of the fermented product of the black red rose:
taking 6g of dried rose black flowers, placing in a 9 x 9cm plate, sterilizing at 121 ℃ for 4h, inoculating activated Aspergillus oryzae with the inoculum size of 10ml, and culturing at 28 ℃ for 7-14 days;
3. alcohol extraction of the fermented product of the black red rose:
the fermented black rose is prepared according to the following steps of 1:20 percent of ethanol is extracted, the concentration of the ethanol is 60 percent, the extraction temperature is 70 ℃, and the extraction time is 3 hours.
After the preparation was completed, the fermented product of the rubia rosea of this comparative example was obtained.
Compounding the fermented product of the rose black obtained in the comparative example 2 with commercially available essential oil and hydrolat, wherein the compounding ratio is as follows:
the fermented product of the red rose of the ink is: and (3) jasmine hydrosol: rose essential oil jasmine essential oil: 1: 0.05: 0.05: 0.05. the fermented product and the compound thereof of the rose and the rubi obtained in the above examples and comparative examples are tested by the following methods:
(1) determination of DPPH radical scavenging Capacity:
1ml of the sample solution +1ml of 0.2mmol/L DPPH was added to the experimental group. 1ml of absolute ethanol +1ml of the sample solution was added to the control group. The blank was added with 1ml of absolute ethanol +1ml of 0.2mmol/L DPPH, and the mixture was allowed to stand at room temperature (25 ℃) for 30 min. Absorbing 300ul at 517nm, measuring the absorbance of the experimental group, and recording as Ai(ii) a The absorbance of the control group was measured and recorded as Aj(ii) a Marking blank group as A0; each sample was tested in 3 replicates.
The calculation formula is as follows: DPPH clearance (%) - [1- (a)i-Aj)/A0]×100％
(2) Superoxide anion radical scavenging capacity determination:
5.7mL of Tris-HCl buffer (50mmol/L, pH 8.2) was added to a 10mL test tube, 0.2mL of the sample was mixed, the mixture was placed in an incubator at 25 ℃ for 10min, and then 0.1mL (10mmol/L) of pyrogallol was addedThe liquid (preheated) is quickly mixed uniformly, then a multifunctional microplate reader is used for measuring the increase value (Aj) of the absorbance value within 1min at the wavelength of 320nm, the increase value of the absorbance within 1min is calculated within the linear range, the reagent is taken, the sample is replaced by equal volume of water, and the increase value (A) of the absorbance within 1min at the wavelength of 320nm is measuredi)。
The calculation formula is as follows: superoxide anion radical clearance (%) ═ ai-Aj)/Ai×100％
(3) Determination of hydroxyl radical (. OH) scavenging Capacity:
hydroxyl radical (. OH) removal test method referring to Fenton reaction method, ferrous sulfate solution (6mmol/L), hydrogen peroxide solution (6mmol/L) and sample are sequentially added into a test tube, each sample is placed statically for 10min after 2mL, then 2mL salicylic acid solution (6mmol/L) is added, and absorbance A is measured at 510nm after standing for 30min0Treating with distilled water instead of sample solution, and measuring absorbance Ax。
The calculation formula is as follows: hydroxyl radical (& OH) clearance (%) - (1-A)0/Ax)×100％
(4) Determination of ABTS clearance:
0.2mL of 7.4mmoL/LABTS and 0.2mL of 2.6mmoL/L K2S2O8Mixing, and reacting at room temperature in the dark for 12h, after the reaction is completed, diluting with 95% ethanol (phosphate buffer solution with pH 7.4, 95% ethanol or methanol) by 40-50 times so that the absorbance of the mixture at 734nm is 0.68-0.72 (working solution).
Diluting the fermentation samples treated differently, respectively sucking 0.2mL, adding 0.8mL diluted working solution, mixing well, standing for reaction for 6min, and rapidly determining light absorption value A at 734nm wavelength0. The same treatment was carried out using 95% ethanol instead of the sample solution, and the absorbance A was measuredx. Each sample was tested in 3 replicates.
The calculation formula is as follows: ABTS free radical clearance (%) ═ 1-A0/Ax×100％
(5) Inhibition rate of tyrosinase activity
And (3) accurately sucking reaction liquid of tyrosinase, PBS and the samples by using a micropipette according to the volumes of the above table, respectively placing the reaction liquid into 4 PE tubes of 10ml, uniformly mixing the reaction liquid (each sample needs an experimental group 1 and an experimental group 2, and a control group can form a control with a plurality of experimental groups), carrying out 37 constant-temperature water bath for 10min, then completely adding 1ml of L-tyrosine into the four groups, reacting for 10min at 37 ℃, and quickly placing the groups in ice water for cooling. The absorbance was measured at 475nm using a microplate reader.
The calculation formula is as follows: tyrosinase activity inhibition rate of 1- (AT)4-AT3)/(AT2-AT1)×100％
AT1Absorbance at 475m measured for control 1;
AT2absorbance at 45m measured for control 2;
AT3test group 1 absorbance at 475 m;
AT4test group 2 absorbance at 475 m;
(6) total reducing power detection
Transferring 1ml of sample into a 10ml EP tube, immediately and rapidly adding 2.5ml (0.2ml/L) of PBS solution and 2.5ml of 1% potassium ferricyanide solution, uniformly mixing, then preserving the temperature for 20min in a 50 ℃ water bath kettle, taking out, adding 2.5ml of 10% trichloroacetic acid solution, adding trichloroacetic acid, centrifuging for 10min at 3500r/min at room temperature if precipitation is generated, sucking 2.5ml of supernatant, and adding 2.5ml of distilled water; if no precipitate is formed after adding trichloroacetic acid, 2.5ml of the solution is taken up and 2.5ml of distilled water is added. Adding 0.5ml of 0.1% ferric trichloride solution, mixing uniformly, and reacting for 10 min. The control group was prepared by replacing the sample solution with the same amount of distilled water, and the other operations were not changed. The result is that the absorbance value is measured under the luminosity of 700nm, and the absorbance of the experimental group and the control group is in direct proportion to the reducing force.
The results of the experiment are shown in table 1:
TABLE 1
As can be seen from Table 1, by compounding with commercially available essential oil and hydrosol with the same components, the performance test results of the Muhong rose composition activated by lucid ganoderma are better, and the Muhong rose composition can play a synergistic effect.
Claims (10)
1. A rose composition comprising a rose ferment and a plant extract in a ratio of 1: 0.05-2, and the rose leavening is obtained by fermenting and extracting a rose raw material with a Ganoderma lucidum strain (Latin under the name of Ganoderma lucidum).
2. The rose composition according to claim 1, wherein the plant extract comprises essential oils containing plant alcohols and/or hydrolat, which is a distillate stock separated during the extraction of essential oils.
3. The rose composition of claim 1 or claim 2, wherein the essential oil comprises one or more of sandalwood essential oil, jasmine essential oil, rose essential oil; the hydrolat comprises one or more of sandalwood hydrolat, jasmine hydrolat and rose hydrolat.
4. The rose composition according to claim 3, wherein the rose fermentation product is compounded with rose essential oil, jasmine essential oil and sandalwood essential oil, and the compounding mass ratio is that the rose fermentation product is: rose essential oil: and (3) jasmine essential oil: sandalwood essential oil 1: 0.05-0.1: 0.05-0.1: 0.05-0.1.
5. The rose composition according to claim 1, wherein the strain of ganoderma lucidum is derived from the collection of common strains of Chinese microorganisms with a collection number of CGMCC 5.1817.
6. The rose composition according to claim 5, wherein the rose ferment is prepared by a process comprising the steps of:
(1) the activated ganoderma lucidum strain is preserved by China general microbiological culture Collection center with the preservation number of CGMCC 5.1817;
(2) fermentation culture: sterilizing dried flos Rosae Rugosae, inoculating activated Ganoderma strain, fermenting and culturing for 7-14 days;
(3) extraction: extracting the product obtained in the step (2) according to the mass ratio of 1:20-40 to obtain the rose fermentation product.
7. The rose composition according to claim 6, wherein the specific method for activating the ganoderma lucidum strain in step (1) is: culturing Ganoderma strain in MEA culture medium at 25-28 deg.C for 5-7 days for activating strain.
8. The rose composition of claim 6, wherein the dried rose flowers in step (2) are dried rose black flowers.
9. The rose composition according to claim 6, wherein the temperature of the sterilization treatment in step (2) is 115 ℃ and 121 ℃ for 4-6 h; the inoculation amount of the ganoderma lucidum strain is 5-10% of the mass of the dried rose flowers; the fermentation culture temperature is 25-28 ℃; the extraction in the step (3) is ethanol extraction, the concentration of the ethanol is 60-90%, the extraction temperature is 50-70 ℃, and the extraction time is 3-5 h.
10. Use of a rose composition according to any of claims 1 to 9 as a cosmetic active ingredient.
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| CN116270826A (en) * | 2023-03-09 | 2023-06-23 | 中国农业科学院麻类研究所 | Method for improving activity of rose in reducing blood sugar and blood fat and application |
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| CN116270826A (en) * | 2023-03-09 | 2023-06-23 | 中国农业科学院麻类研究所 | Method for improving activity of rose in reducing blood sugar and blood fat and application |
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