CN115006297A - Revival grass fermentation extracting solution, preparation method and application - Google Patents
Revival grass fermentation extracting solution, preparation method and application Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9741—Pteridophyta [ferns]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Abstract
The invention relates to the field of cosmetics, and particularly discloses a revived grass fermentation extracting solution, a preparation method and application thereof, wherein the revived grass fermentation extracting solution has higher active substance content and stronger antioxidant activity compared with the conventional revived grass extracting solution. Moreover, the preparation method provided by the embodiment of the invention is simple and has a wide application prospect.
Description
Technical Field
The invention relates to the field of cosmetics, and particularly relates to a revived grass fermentation extracting solution, a preparation method and application.
Background
Revival grass, Chinese scientific name Selaginella tamariscina, also known as Jiu-death Senecio Ccannabifolius, Rohdea japonica, houseleek flower, etc. The root of the revival grass grows only at the base of the stem, the root is branched, and the main stem begins to branch like a pinnate branch or an unequal binary branch from the middle part. As chemical components in the revival grass serving as a desert plant mainly comprise flavonoids, alkynol, alkaloids, glycosides and the like, the desert plant has good effects of resisting oxidation, resisting bacteria, reducing blood sugar and the like.
At present, the active ingredients in revived grass are mainly extracted by a hot water extraction method, an organic reagent extraction method and the like in the prior art. However, the existing methods consume a large amount of organic reagents, have high energy consumption and high cost in practical application, easily cause loss of active ingredients in the extraction process, reduce the content and the effect of the active ingredients of the revived grass extracting solution, and further cause low extraction efficiency.
Therefore, the above technical solutions have the following disadvantages in practical use: most of the revival grass extraction methods in the prior art have the problems of high energy consumption, high cost and low extraction efficiency.
Disclosure of Invention
The invention aims to provide a revival grass fermentation extracting solution to solve the problems of large energy consumption, high cost and low extraction efficiency of a revival grass extraction mode in the prior art in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a revival grass fermentation extract is prepared by taking a revival grass fermentation culture medium as a raw material and respectively carrying out aerobic fermentation and anaerobic fermentation on the revival grass fermentation culture medium by inoculating saccharomyces cerevisiae; wherein the revival grass fermentation medium comprises the following raw materials: revival grass powder, protein powder, glucose and water.
It should be noted that the prior art mainly adopts hot water extraction, organic reagent extraction and the like to extract active ingredients from revived grass, which not only consumes a large amount of organic reagents, but also has high energy consumption and high cost in practical application, and easily causes active ingredient loss in the extraction process, resulting in low extraction efficiency. In the embodiment of the invention, the revived grass fermentation extracting solution is obtained by fermenting the revived grass by using the saccharomyces cerevisiae, can be directly used as cosmetics or added into the cosmetics as an additive, and has the effects of oxidation resistance, bacteriostasis and the like. Moreover, the fermentation extraction method is an effective means for extracting the active ingredients of the plants, the energy consumption is low, the pollution is less, the cost is low, the content of the active ingredients in the final revived grass fermentation extracting solution is high, the active ingredients cannot be damaged in the extraction process, and microorganisms can grow by using the active ingredients in the plants and metabolize new active ingredients in the fermentation process, so that the effect of the final revived grass fermentation extracting solution is enhanced in many aspects.
Another object of the embodiments of the present invention is to provide a method for preparing the revival grass fermentation extract, which specifically comprises the following steps:
inoculating saccharomyces cerevisiae into the revival grass fermentation culture medium for aerobic fermentation, then carrying out anaerobic fermentation, standing and cooling after sterilization, and centrifuging to remove precipitates to obtain the revival grass fermentation extracting solution.
Another object of the embodiments of the present invention is to provide a revival grass fermented extract prepared by the above method for preparing a revival grass fermented extract.
Another object of the embodiments of the present invention is to provide an application of the revival grass fermentation extract in the preparation of cosmetics and/or skin care products.
Compared with the prior art, the invention has the beneficial effects that:
compared with the conventional revival grass extracting solution, the revival grass fermentation extracting solution provided by the invention has higher active substance content and stronger antioxidant activity, and can be directly used as cosmetics or added into the cosmetics as an additive by carrying out aerobic fermentation and anaerobic fermentation on a revival grass fermentation culture medium by using saccharomyces cerevisiae at one time.
Drawings
FIG. 1 is a graph illustrating the result of DPPH radical scavenging experiments according to an embodiment of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the following specific embodiments and the accompanying drawings. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention.
The revival grass fermentation extracting solution provided by the embodiment of the invention is prepared by taking a revival grass fermentation culture medium as a raw material and respectively carrying out aerobic fermentation and anaerobic fermentation on the revival grass fermentation culture medium by inoculating saccharomyces cerevisiae; wherein the revival grass fermentation medium comprises the following raw materials: revival grass powder, protein powder, glucose and water.
As another preferred embodiment of the present invention, the water may be any one selected from purified water, mineral water, distilled water, deionized water, and soft water, which is not limited herein and may be selected as needed.
Preferably, the water is deionized water.
As another preferred embodiment of the invention, the revival grass fermentation medium is prepared by mixing revival grass powder, protein powder, glucose and water and then sterilizing.
As another preferred embodiment of the invention, when the revival grass fermentation medium is prepared, the sterilization is high-pressure moist heat sterilization, and the specific sterilization temperature is 105-125 ℃, and the sterilization time is 10-40 min.
As another preferred embodiment of the invention, the revival grass fermentation medium comprises the following raw materials in parts by weight: 3-30 parts of revival grass powder, 0.3-6 parts of protein powder, 1.5-15 parts of glucose and 350 parts of water.
As another preferred embodiment of the invention, the revived grass powder is prepared by drying and crushing revived grass into 40-200 meshes.
Preferably, the preparation method of the revival grass fermentation medium comprises the following steps:
drying the revival grass, then crushing the dried revival grass into 40-200 meshes to obtain revival grass powder, weighing 3-30 g of revival grass powder, adding 1.5-15 g of glucose, 0.3-6 g of protein powder and 300 g of deionized water, uniformly stirring, placing the mixture into a 500 ml triangular flask, sealing the flask by using a sealing film, and then carrying out high-pressure moist heat sterilization at the sterilization temperature of 105-125 ℃ for 10-40 min; and (4) after the sterilization is finished, standing to normal temperature to obtain the revival grass fermentation medium.
In another preferred embodiment of the present invention, the saccharomyces cerevisiae comprises any one of saccharomyces cerevisiae, yellow wine yeast, sake yeast, and the like.
In the embodiment of the invention, the revived grass fermentation extracting solution obtained by carrying out aerobic fermentation and anaerobic fermentation on the revived grass fermentation culture medium by using saccharomyces cerevisiae at one time has higher active substance content and stronger antioxidant activity, and can be directly used as cosmetics or added into the cosmetics as an additive.
The embodiment of the invention also provides a preparation method of the revival grass fermentation extracting solution, which comprises the following steps:
inoculating saccharomyces cerevisiae into a revival grass fermentation culture medium for aerobic fermentation, then carrying out anaerobic fermentation, standing and cooling after sterilization, and centrifuging to remove precipitates to obtain the revival grass fermentation extracting solution.
In another preferred embodiment of the present invention, the method for preparing the revival grass fermentation extract further comprises the step of adding a preservative after removing the precipitate by centrifugation and mixing the mixture uniformly. The preservative can be a preservative which can be used for cosmetics in the prior art, is selected according to requirements, and is not described in detail herein. Of course, the specific amount of the preservative is selected according to the needs, and the preservative may be directly used as a cosmetic without being added.
As another preferred embodiment of the invention, in the preparation method of the revived grass fermentation extract, the sterilization temperature is 60-100 ℃, and the sterilization time is 30-120 min.
In another preferred embodiment of the present invention, the inoculation amount of the revived grass fermentation medium for aerobic fermentation with saccharomyces cerevisiae is 1 wt% to 20 wt%, that is, 1 wt% to 20 wt% of saccharomyces cerevisiae is inoculated to the revived grass fermentation medium, and the concentration of the saccharomyces cerevisiae solution is 10 4 -10 9 CFU/mL。
As another preferred embodiment of the invention, in the preparation method of the revived grass fermentation extract, the aerobic fermentation is performed for 4-48h at constant temperature of 20-35 ℃ with shaking and fermentation culture.
As another preferred embodiment of the invention, the rotation speed of constant temperature shaking is 100r/min-200r/min, specifically, the aerobic fermentation is cultured in a constant temperature shaking incubator for 4-48h, the culture temperature is 20-35 ℃, and the rotation speed of the incubator is 100-200 r/min. This stage is the aerobic fermentation stage.
As another preferred embodiment of the present invention, the anaerobic fermentation is a fermentation culture at 20-35 ℃ for 24-120 h. Specifically, the culture medium is moved into a constant temperature incubator for anaerobic fermentation, the culture temperature is 20-35 ℃, and the culture time is 24-120 h.
As another preferred embodiment of the invention, in the preparation method of the revived grass fermentation extract, the rotation speed of the centrifugation is 2000r/min-10000r/min, and the time of the centrifugation is 5min-40 min.
Preferably, the preparation method of the revival grass fermentation extract comprises the following steps:
(1) inoculating Saccharomyces cerevisiae into the revived grass fermentation culture medium, wherein the concentration of the bacterial liquid is 10 4 -10 9 CFU/mL, the inoculation amount is 1% -20%. After the inoculation is finished, the triangular flask is placed in a constant-temperature shaking incubator for culture for 4-48h, the culture temperature is 20-35 ℃, and the rotation speed of the incubator is 100-200 r/min. This stage is the aerobic fermentation stage.
(2) After the aerobic fermentation stage is finished, the triangular flask is moved into a constant temperature incubator for anaerobic fermentation, the culture temperature is 20-35 ℃, and the culture time is 24-120 h.
(3) After the anaerobic fermentation is finished, sterilizing the triangular flask at 60-100 deg.C for 30-120 min. After sterilization, standing, cooling and centrifuging, wherein the centrifugal speed is 2000-10000r/min, and the centrifugal time is 5-40 min. And filtering the supernatant to obtain the revived grass fermentation extract. The revival grass fermentation extract is preserved or added into cosmetics, namely revival grass fermentation cosmetics, which is also within the protection scope of the invention.
The embodiment of the invention also provides a revival grass fermentation extracting solution prepared by the preparation method of the revival grass fermentation extracting solution.
The embodiment of the invention also provides application of the revival grass fermentation extracting solution in preparation of cosmetics and/or skin care products.
The technical effects of the extract from the fermentation of revival grass of the present invention will be further described below by referring to specific examples.
Example 1
A revival grass fermentation extract is prepared by the following steps:
drying the resurrection grass, crushing to 100 meshes to obtain resurrection grass powder, weighing 15 g of resurrection grass powder, adding 6 g of glucose, 3 g of protein powder and 300 g of deionized water, uniformly stirring, placing in a 500 ml triangular flask, sealing by using a sealing film, and performing high-pressure damp-heat sterilization at the sterilization temperature of 121 ℃ for 15 min. After the sterilization is finished, standing to normal temperature, inoculating saccharomyces cerevisiae, wherein the concentration of the bacterial liquid is 10 7 CFU/mL, the inoculation amount is 5 wt%. After the inoculation is finished, placing the triangular flask in a constant-temperature shaking incubator for aerobic fermentation culture for 24 hours, wherein the culture temperature is 28 ℃, and the rotation speed of the incubator is 160 r/min. After the aerobic fermentation stage is finished, the triangular flask is moved to a constant-temperature incubator for anaerobic fermentation, the culture temperature is 25 ℃, and the culture time is 72 hours. After the anaerobic fermentation is finished, the triangular flask is sterilized at 80 ℃ for 40 min. And after the sterilization is finished, standing, cooling and centrifuging at the rotating speed of 5000r/min for 30 min. Mixing the supernatantFiltering the solution to obtain the revived grass fermentation extract.
Example 2
A revival grass fermentation extract is prepared by taking a revival grass fermentation culture medium as a raw material, and respectively carrying out aerobic fermentation and anaerobic fermentation on the revival grass fermentation culture medium by inoculating saccharomyces cerevisiae; wherein the revival grass fermentation medium comprises the following raw materials: 3 g of revival grass powder, 0.3 g of protein powder, 1.5 g of glucose and 250 g of deionized water. The revival grass fermentation medium is prepared by mixing revival grass powder, protein powder, glucose and water, and then performing high-pressure damp-heat sterilization, wherein the sterilization temperature is 105 ℃, and the sterilization time is 10 min. The saccharomyces cerevisiae is saccharomyces cerevisiae.
In the embodiment of the invention, the preparation method of the revival grass fermentation extracting solution specifically comprises the following steps:
inoculating saccharomyces cerevisiae (the inoculation amount is 1 wt%) into the revived grass fermentation culture medium, and culturing for 4h in a constant-temperature shaking incubator at the culture temperature of 20 ℃ and the rotation speed of the incubator of 100 r/min; after the aerobic fermentation stage is finished, transferring the culture medium into a constant-temperature incubator for anaerobic fermentation, wherein the culture temperature is 20 ℃, and the culture time is 24 hours; and (4) sterilizing after the anaerobic fermentation is finished, wherein the sterilization temperature is 60 ℃, and the sterilization time is 30 min. After the sterilization is finished, standing, cooling and centrifuging at the rotating speed of 2000r/min for 40 min. And filtering the supernatant to obtain the revived grass fermentation extracting solution.
Example 3
A revival grass fermentation extract is prepared by taking a revival grass fermentation culture medium as a raw material and respectively carrying out aerobic fermentation and anaerobic fermentation on the revival grass fermentation culture medium by inoculating saccharomyces cerevisiae; wherein the revival grass fermentation medium comprises the following raw materials: 30 g of revival grass powder, 6 g of protein powder, 15 g of glucose and 350 g of deionized water. The revived grass fermentation medium is prepared by mixing revived grass powder, protein powder and glucose with water, and performing high-pressure moist heat sterilization, wherein the specific sterilization temperature is 125 ℃, and the sterilization time is 40 min. The saccharomyces cerevisiae is saccharomyces cerevisiae.
In the embodiment of the invention, the preparation method of the revival grass fermentation extracting solution specifically comprises the following steps:
inoculating saccharomyces cerevisiae (the inoculation amount is 20 wt%) into the revived grass fermentation culture medium, and culturing in a constant-temperature shaking incubator for 48h, wherein the culture temperature is 35 ℃, and the rotation speed of the incubator is 200 r/min; after the aerobic fermentation stage is finished, transferring the mixture into a constant-temperature incubator for anaerobic fermentation, wherein the culture temperature is 35 ℃, and the culture time is 120 h; and (4) sterilizing after the anaerobic fermentation is finished, wherein the sterilization temperature is 100 ℃, and the sterilization time is 120 min. After sterilization, standing, cooling and centrifuging at 10000r/min for 5 min. And filtering the supernatant to obtain the revived grass fermentation extracting solution.
Example 4
A revival grass fermentation extract is prepared by taking a revival grass fermentation culture medium as a raw material, and respectively carrying out aerobic fermentation and anaerobic fermentation on the revival grass fermentation culture medium by inoculating saccharomyces cerevisiae; wherein the revival grass fermentation medium comprises the following raw materials: 20 g of revival grass powder, 2 g of protein powder, 5 g of glucose and 280 g of deionized water. The revived grass fermentation medium is prepared by mixing revived grass powder, protein powder and glucose with water, and performing high-pressure moist heat sterilization, wherein the specific sterilization temperature is 110 ℃, and the sterilization time is 20 min. The saccharomyces cerevisiae is saccharomyces cerevisiae.
In the embodiment of the invention, the preparation method of the revival grass fermentation extract is the same as that of the embodiment 2.
Example 5
A revival grass fermentation extract is prepared by taking a revival grass fermentation culture medium as a raw material, and respectively carrying out aerobic fermentation and anaerobic fermentation on the revival grass fermentation culture medium by inoculating saccharomyces cerevisiae; wherein the revival grass fermentation medium comprises the following raw materials: 15 g of revival grass powder, 4 g of protein powder, 8 g of glucose and 300 g of deionized water. The revived grass fermentation medium is prepared by mixing revived grass powder, protein powder and glucose with water, and performing high-pressure moist heat sterilization, wherein the sterilization temperature is 115 ℃ and the sterilization time is 25 min. The saccharomyces cerevisiae is saccharomyces cerevisiae.
In the embodiment of the invention, the preparation method of the revival grass fermentation extracting solution specifically comprises the following steps:
inoculating saccharomyces cerevisiae (the inoculation amount is 10 wt%) into the revived grass fermentation culture medium, and culturing in a constant-temperature shaking incubator for 20h, wherein the culture temperature is 28 ℃, and the rotation speed of the incubator is 150 r/min; after the aerobic fermentation stage is finished, transferring the mixture into a constant-temperature incubator for anaerobic fermentation, wherein the culture temperature is 28 ℃, and the culture time is 80 hours; and (4) sterilizing after the anaerobic fermentation is finished, wherein the sterilization temperature is 80 ℃, and the sterilization time is 90 min. And after the sterilization is finished, standing, cooling and centrifuging, wherein the centrifugal rotating speed is 6000r/min, and the centrifugal time is 20 min. And filtering the supernatant to obtain the revived grass fermentation extracting solution.
Example 6
The procedure of example 2 was repeated except that the Saccharomyces cerevisiae was a mixture of Saccharomyces cerevisiae, and Saccharomyces sake.
Example 7
Compared with example 2, except that the saccharomyces cerevisiae is red wine yeast, the method is the same as the example 2.
Example 8
Compared with the example 2, the saccharomyces cerevisiae is the same as the example 2 except that the saccharomyces cerevisiae is yellow wine yeast.
Example 9
Compared with example 2, the method is the same as example 2 except that the saccharomyces cerevisiae is sake yeast.
Example 10
The procedure of example 1 was repeated except that the particle size of the revived grass powder was 40 mesh as compared with example 1.
Example 11
The procedure of example 1 was repeated except that the particle size of the revived grass powder was 80 mesh as compared with example 1.
Example 12
The procedure of example 1 was repeated except that the particle size of the revived grass powder was 200 mesh as compared with example 1.
Example 13
The same as example 1 except that the deionized water was replaced with purified water as compared with example 1.
Example 14
The same as example 1 except that deionized water was replaced with mineral water, as compared with example 1.
Example 15
The same as example 1 except that the deionized water was replaced with soft water as compared with example 1.
Example 16
Compared with example 1, except that the inoculation amount of the saccharomyces cerevisiae for aerobic fermentation is 4 wt%, and the bacterial liquid concentration of the saccharomyces cerevisiae is 10 4 The procedure was repeated in the same manner as in example 1 except for CFU/mL.
Example 17
Compared with example 1, except that the inoculation amount of the saccharomyces cerevisiae for aerobic fermentation is 12 wt%, and the bacterial liquid concentration of the saccharomyces cerevisiae is 10 6 The procedure was repeated in the same manner as in example 1 except for CFU/mL.
Example 18
Compared with example 1, except that the inoculation amount of the saccharomyces cerevisiae for aerobic fermentation is 20 wt%, and the concentration of the saccharomyces cerevisiae liquid is 10 9 The procedure was repeated except for using CFU/mL in the same manner as in example 1.
Example 19
A revival grass fermented cosmetic is prepared by the following steps:
(1) drying the resurrection grass, then crushing to 40-200 meshes to obtain resurrection grass powder, weighing 3-30 g of resurrection grass powder, adding 1.5-15 g of glucose, 0.3-6 g of protein powder and 300 g of deionized water, uniformly stirring, placing in a 500 ml triangular flask, sealing by using a sealing film, and then carrying out high-pressure moist heat sterilization at the sterilization temperature of 105-; and (4) after the sterilization is finished, standing to normal temperature to obtain the revival grass fermentation medium.
(2) Inoculating saccharomyces cerevisiae into the revived grass fermentation culture medium obtained in the step (1), wherein the concentration of the bacterial liquid is 10 4 -10 9 CFU/mL, the inoculation amount is 1% -20%. After the inoculation is finishedThe triangular flask is placed in a constant-temperature shaking incubator for culture for 4-48h, the culture temperature is 20-35 ℃, and the rotation speed of the incubator is 100-. This stage is the aerobic fermentation stage.
(3) After the aerobic fermentation stage in the step (2) is finished, the triangular flask is moved to a constant temperature incubator for anaerobic fermentation, the culture temperature is 20-35 ℃, and the culture time is 24-120 h.
(4) And (4) after the anaerobic fermentation in the step (3) is finished, sterilizing the triangular flask at the temperature of 60-100 ℃ for 30-120 min. After sterilization, standing, cooling and centrifuging, wherein the centrifugal speed is 2000-10000r/min, and the centrifugal time is 5-40 min. And filtering the supernatant to obtain the revived grass fermentation extract. And performing corrosion prevention on the revived grass fermentation extracting solution or adding the revived grass fermentation extracting solution into cosmetics to obtain the revived grass fermentation cosmetics.
Firstly, the method comprises the following steps: detection of antioxidant property of revival grass fermentation extract
DPPH is an early synthetic organic radical commonly used to evaluate the hydrogen donating ability of antioxidants, is very stable in organic solvents, is purple in color, and has a characteristic absorption peak at 517nm, when encountering radical scavengers, the lone pair of DPPH is paired to discolor it, i.e., the absorbance at the wavelength of maximum absorption becomes small. Therefore, the effect of the sample on DPPH radical scavenging can be evaluated by measuring the change in absorbance.
Different amounts of the revival grass fermentation extract prepared in example 1 were dissolved in deionized water to prepare a series of solutions to be tested with volume percentage concentrations of 20%, 40%, 60%, 80%, 100%, with vitamin C as a positive control.
The specific experimental steps of the DPPH free radical scavenging experiment are as follows:
(1) taking the same volume (3mL) of the solution to be detected and 2X 10 -4 mixing (A) with a solution of DPPH in mol/L 1 A tube);
(2) taking equal volume (3mL) of absolute ethanol (solvent of the test substance) and 2X 10 -4 mixing (A) with a solution of DPPH in mol/L 2 A tube);
(3) mixing the same volume (3mL) of anhydrous ethanol with the solution to be detected (A) 3 A tube);
(4) after 30min of reaction, A was measured at 517nm 1 Pipe, A 2 Pipe, A 3 Tube absorbance values.
The clearance rate is calculated by the formula: clearance (%) - (A) 2 +A 3 )-A 1 ]/A 2 (1)
And (3) taking the volume percentage concentration of the liquid to be detected as an abscissa and the clearance as an ordinate, and making a DPPH free radical scavenging action curve, wherein A: revival grass fermentation extracting solutions with different concentrations, B: vitamin C (1 mg/mL).
As can be seen from FIG. 1, the IC50 for scavenging DPPH free radicals of the resuscitated grass fermented extract obtained in example 1 was 70.71%, which indicates that the resuscitated grass fermented extract has strong antioxidant activity.
II, secondly: safety detection of revival grass fermentation extracting solution
The human body patch test is mainly used for detecting the irritation of the final cosmetic product or raw materials. The invention carries out a closed patch test on the revived grass fermentation extract obtained in the example 1, aiming at evaluating the potential skin irritation.
1. Test object
Suitable volunteers were selected for 30 persons, and were randomly selected in the age range of 18-60 years.
2. Test method
The liquid sample, 0.2mL to 0.025mL, was dropped onto the filter paper sheet, which was then placed in the plaque tester. A blank control is set for each sample, and an equal amount of sample solvent, such as distilled water or olive oil (distilled water is used in this example), is added to the control chamber.
The test part is selected as the back of a human body, and the spot tester is fixedly attached to the back of the testee by using a non-irritant adhesive tape. The test period lasted 24 h. In order to ensure the accuracy, credibility and scientificity of test results, the volunteers cannot remove the spot tester or make the tested part contact water according to the requirements during the test. And removing the spot tester after 24h, standing for 30min, waiting for the indentation to disappear, and observing the reaction of the skin. If the test result is negative, the test needs to be observed once more at 24h and 48h after the patch test.
3. Test results
The results of the patch test are shown in Table 1, and the symbols in Table 1 have the following meanings: "-negative reaction; "±" ═ suspicious reaction: only faint erythema; "+" ═ weak positive reaction (erythema reaction): erythema, infiltration, edema, and possibly pimples; "+ +", strong positive reaction (herpes response); erythema, infiltration, edema, papules, herpes; the reaction may be beyond the test area; "+ + + +" -very strong positive reaction (fusogenic herpes response); obvious erythema, severe infiltration, edema, and fusional herpes; the reaction was beyond the test area.
TABLE 1 Patch test results of the revival grass fermentation extract obtained in example 1
As can be seen from table 1: the revival grass fermentation extract obtained in the embodiment 1 does not produce suspicious reactions, which shows that the revival grass fermentation extract provided by the invention has safety and does not bring adverse reactions to human bodies.
The method aims at the defects of low extraction efficiency, high components, high energy consumption and the like of active ingredients in the revival grass in the prior art, and utilizes the saccharomyces cerevisiae to ferment the revival grass to obtain the revival grass fermentation extracting solution, and compared with the conventional revival grass extracting solution, the revival grass fermentation extracting solution has higher active substance content and stronger antioxidant activity. In the fermentation process, no organic reagent is added, the fermentation condition is mild, the obtained revival grass fermentation extract has higher safety and does not cause irritation to skin, and the extract can be directly used as cosmetics or added into the cosmetics as an additive and has the effects of resisting oxidation, inhibiting bacteria and the like.
While the preferred embodiments of the present invention have been described in detail, the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications of the invention may be made without departing from the scope of the invention.
Claims (10)
1. The revival grass fermentation extracting solution is characterized in that the revival grass fermentation extracting solution is prepared by taking a revival grass fermentation culture medium as a raw material and respectively carrying out aerobic fermentation and anaerobic fermentation on the revival grass fermentation culture medium by inoculating saccharomyces cerevisiae; wherein the revival grass fermentation medium comprises the following raw materials: revival grass powder, protein powder, glucose and water.
2. The revival grass fermentation extract as claimed in claim 1, wherein the revival grass fermentation medium is prepared by mixing revival grass powder, protein powder, glucose and water, and sterilizing.
3. The revival grass fermentation extracting solution as claimed in claim 1, wherein the revival grass fermentation culture medium comprises the following raw materials in parts by weight: 3-30 parts of revival grass powder, 0.3-6 parts of protein powder, 1.5-15 parts of glucose and 350 parts of water.
4. The revival grass fermentation extract as claimed in claim 1, wherein the saccharomyces cerevisiae includes any one of brewer's yeast, red wine yeast, yellow wine yeast and sake yeast.
5. A method for preparing revived grass fermentation extract according to any one of claims 1 to 4, comprising the steps of: inoculating saccharomyces cerevisiae into the revival grass fermentation culture medium for aerobic fermentation, then carrying out anaerobic fermentation, standing and cooling after sterilization, and centrifuging to remove precipitates to obtain the revival grass fermentation extracting solution.
6. The method for preparing revived grass fermentation extract according to claim 5, wherein the inoculation amount of saccharomyces cerevisiae when saccharomyces cerevisiae is inoculated into a revived grass fermentation medium for aerobic fermentation is 1 wt% -20 wt%, and the concentration of saccharomyces cerevisiae bacterial liquid is 10 4 -10 9 CFU/mL。
7. The method for preparing revival grass fermentation extracting solution as claimed in claim 5, characterized in that in the method for preparing revival grass fermentation extracting solution, the aerobic fermentation is performed for 4-48h at constant temperature of 20-35 ℃ with shaking and fermentation culture.
8. The method for preparing revival grass fermentation extract according to claim 5, wherein the anaerobic fermentation is performed at 20-35 ℃ for 24-120 h.
9. A revived grass fermented extract prepared by the method for preparing a revived grass fermented extract according to claim 5, 6, 7 or 8.
10. Use of a revival grass fermented extract according to claim 1 or 2 or 3 or 4 or 9 for the preparation of a cosmetic and/or dermatological product.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115590805A (en) * | 2022-11-09 | 2023-01-13 | 珠海市科美优肽生物科技有限公司（Cn） | Microbial fermentation process for preparing active cosmetics |
| CN116602898A (en) * | 2023-06-08 | 2023-08-18 | 深圳杉海创新技术有限公司 | Reviving grass extract fermentation liquor and preparation method and application thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115590805A (en) * | 2022-11-09 | 2023-01-13 | 珠海市科美优肽生物科技有限公司（Cn） | Microbial fermentation process for preparing active cosmetics |
| CN116602898A (en) * | 2023-06-08 | 2023-08-18 | 深圳杉海创新技术有限公司 | Reviving grass extract fermentation liquor and preparation method and application thereof |
| CN116602898B (en) * | 2023-06-08 | 2024-03-29 | 深圳杉海创新技术有限公司 | Reviving grass extract fermentation liquor and preparation method and application thereof |
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