CN111616997A - Junshan honeysuckle tea fermentation raw stock and preparation method and application thereof - Google Patents
Junshan honeysuckle tea fermentation raw stock and preparation method and application thereof Download PDFInfo
- Publication number
- CN111616997A CN111616997A CN202010503551.5A CN202010503551A CN111616997A CN 111616997 A CN111616997 A CN 111616997A CN 202010503551 A CN202010503551 A CN 202010503551A CN 111616997 A CN111616997 A CN 111616997A
- Authority
- CN
- China
- Prior art keywords
- junshan
- fermentation
- tea
- silver needle
- fermentation raw
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000855 fermentation Methods 0.000 title claims abstract description 79
- 230000004151 fermentation Effects 0.000 title claims abstract description 79
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 241001122767 Theaceae Species 0.000 title claims description 67
- 241000205585 Aquilegia canadensis Species 0.000 title description 2
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims abstract description 49
- 229910052709 silver Inorganic materials 0.000 claims abstract description 49
- 239000004332 silver Substances 0.000 claims abstract description 49
- 238000000034 method Methods 0.000 claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 17
- 239000000843 powder Substances 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 10
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 10
- 238000002156 mixing Methods 0.000 claims abstract description 5
- 239000006228 supernatant Substances 0.000 claims abstract description 4
- 230000001954 sterilising effect Effects 0.000 claims abstract description 3
- 239000000047 product Substances 0.000 claims description 17
- 230000008569 process Effects 0.000 claims description 11
- 239000001963 growth medium Substances 0.000 claims description 10
- 238000005119 centrifugation Methods 0.000 claims description 9
- 238000009630 liquid culture Methods 0.000 claims description 6
- 241000894006 Bacteria Species 0.000 claims description 4
- 238000000746 purification Methods 0.000 claims description 4
- 240000004550 Hesperantha coccinea Species 0.000 claims description 3
- 230000003213 activating effect Effects 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- 150000004676 glycans Chemical class 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 239000002609 medium Substances 0.000 claims description 3
- 239000006872 mrs medium Substances 0.000 claims description 3
- 238000007747 plating Methods 0.000 claims description 3
- 229920001282 polysaccharide Polymers 0.000 claims description 3
- 239000005017 polysaccharide Substances 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 102000010911 Enzyme Precursors Human genes 0.000 claims description 2
- 108010062466 Enzyme Precursors Proteins 0.000 claims description 2
- 239000006210 lotion Substances 0.000 claims description 2
- 241000235342 Saccharomycetes Species 0.000 claims 1
- 238000001035 drying Methods 0.000 claims 1
- 229930003935 flavonoid Natural products 0.000 claims 1
- 150000002215 flavonoids Chemical class 0.000 claims 1
- 235000017173 flavonoids Nutrition 0.000 claims 1
- 238000000227 grinding Methods 0.000 claims 1
- 239000011812 mixed powder Substances 0.000 claims 1
- 150000002989 phenols Chemical class 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 16
- 238000000605 extraction Methods 0.000 abstract description 2
- 244000269722 Thea sinensis Species 0.000 abstract 5
- 235000013616 tea Nutrition 0.000 description 48
- 239000000523 sample Substances 0.000 description 22
- 238000012360 testing method Methods 0.000 description 20
- 102000003425 Tyrosinase Human genes 0.000 description 13
- 108060008724 Tyrosinase Proteins 0.000 description 13
- 238000006243 chemical reaction Methods 0.000 description 11
- 239000002537 cosmetic Substances 0.000 description 9
- 230000002087 whitening effect Effects 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 239000000126 substance Substances 0.000 description 7
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 6
- BJRNKVDFDLYUGJ-RMPHRYRLSA-N hydroquinone O-beta-D-glucopyranoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-RMPHRYRLSA-N 0.000 description 6
- 230000005764 inhibitory process Effects 0.000 description 6
- 206010015150 Erythema Diseases 0.000 description 5
- 231100000321 erythema Toxicity 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- XQFRJNBWHJMXHO-RRKCRQDMSA-N IDUR Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(I)=C1 XQFRJNBWHJMXHO-RRKCRQDMSA-N 0.000 description 4
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- 206010030113 Oedema Diseases 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 229960000271 arbutin Drugs 0.000 description 3
- 210000000805 cytoplasm Anatomy 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000008595 infiltration Effects 0.000 description 3
- 238000001764 infiltration Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- BJRNKVDFDLYUGJ-UHFFFAOYSA-N p-hydroxyphenyl beta-D-alloside Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-UHFFFAOYSA-N 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 2
- 206010000496 acne Diseases 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 238000003287 bathing Methods 0.000 description 2
- 229930003944 flavone Natural products 0.000 description 2
- 150000002212 flavone derivatives Chemical class 0.000 description 2
- 235000011949 flavones Nutrition 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 230000007794 irritation Effects 0.000 description 2
- 230000003061 melanogenesis Effects 0.000 description 2
- 239000000419 plant extract Substances 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 2
- OCZVHBZNPVABKX-UHFFFAOYSA-N 1,1-diphenyl-2-(2,4,6-trinitrophenyl)hydrazine;ethanol Chemical compound CCO.[O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1NN(C=1C=CC=CC=1)C1=CC=CC=C1 OCZVHBZNPVABKX-UHFFFAOYSA-N 0.000 description 1
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- 206010067484 Adverse reaction Diseases 0.000 description 1
- 101100191768 Caenorhabditis elegans pbs-4 gene Proteins 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000721047 Danaus plexippus Species 0.000 description 1
- 208000003351 Melanosis Diseases 0.000 description 1
- 230000002292 Radical scavenging effect Effects 0.000 description 1
- 206010040880 Skin irritation Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 206010061428 decreased appetite Diseases 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 102000038379 digestive enzymes Human genes 0.000 description 1
- 108091007734 digestive enzymes Proteins 0.000 description 1
- MGJZITXUQXWAKY-UHFFFAOYSA-N diphenyl-(2,4,6-trinitrophenyl)iminoazanium Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1N=[N+](C=1C=CC=CC=1)C1=CC=CC=C1 MGJZITXUQXWAKY-UHFFFAOYSA-N 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 230000000799 fusogenic effect Effects 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000007373 indentation Methods 0.000 description 1
- 239000002085 irritant Substances 0.000 description 1
- 231100000021 irritant Toxicity 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 230000008099 melanin synthesis Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 238000000643 oven drying Methods 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 231100000475 skin irritation Toxicity 0.000 description 1
- 230000036556 skin irritation Effects 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 229960004441 tyrosine Drugs 0.000 description 1
- 235000020338 yellow tea Nutrition 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/20—Chemical, physico-chemical or functional or structural properties of the composition as a whole
- A61K2800/30—Characterized by the absence of a particular group of ingredients
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Dermatology (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a Junshan silver needle tea fermentation raw stock and a preparation method and application thereof, wherein the preparation method comprises the following steps: mixing the powder of the Junshan Yinzi tea leaves, water and a zymophyte liquid to obtain an initial system, fermenting to obtain a fermentation liquid, and sterilizing and centrifuging the obtained fermentation liquid to obtain a supernatant, namely the Junshan Yinzi tea fermentation raw stock. The method adopts yellow wine yeast to ferment the Junshan silver needle tea leaves, retains all functional components and activity of the Junshan silver needle tea leaves, and avoids loss of active components caused by an extraction method.
Description
Technical Field
The invention relates to a fermentation product, in particular to a fermentation product with a skin care function.
Background
Junshan Yinzi is one of famous Chinese teas, and belongs to yellow tea in six major teas. The tea is thin and needle-like, so it is named as Junshan Yinzi. The Junshan silver needle is made of bud heads, and the tea body is full of cotton wool, and has bright color. The tea bud is healthy and strong, the length and the size are uniform, the inner surface of the tea bud is golden yellow, the outer layer of the tea bud is completely exposed, the tea bud is wrapped firmly, the appearance of the tea bud is very similar to that of a root silver needle, and the tea bud is elegantly called as 'gold inlaid jade'. The Junshan Yinpi contains a large amount of digestive enzymes, is beneficial to the spleen and stomach, and can be drunk by people with dyspepsia and inappetence.
So far, no Junshan silver needle is applied to skin care products. Moreover, most skin care products in the industry are not fermented skin care products and are mainly prepared by the traditional process, but the traditional process is limited by ancient thinking and is difficult to break through, and even the traditional process is currently found to extract plant extracts in a plurality of skin care products, so that not only medicinal materials are seriously consumed, but also the sustainable development is realized, even the obtained substances have limited types and quantity, and the unknown irritation is often caused, so that a new technology is considered. At present, many of the skin care products are claimed to be fermented, and the actual commercial products are only a little fermentation source components added to the formula of the skin care products, so that the content of the fermentation products in the formula is only 1-2%, other components are traditional chemical substances, while many plant extracts contain a small amount of substances, and the substances are continuously added in the formula at the low proportion, so that poor effects are naturally obtained, and the appearance state is transparent and clear, but has no significance to consumers.
The statements in the background section are merely prior art as they are known to the inventors and do not, of course, represent prior art in the field.
Disclosure of Invention
The invention aims to provide a fermentation raw stock of Junshan silver needle tea aiming at one or more problems in the prior art;
the invention also aims to provide a preparation method of the fermentation raw stock prepared by the method;
the invention also aims to provide application of the fermentation raw pulp.
The purpose of the invention is realized by the following technical scheme:
a fermentation raw stock of Junshan silver needle tea is prepared by fermenting Junshan silver needle tea dried at 70-80 deg.C.
According to one aspect of the invention, the fermentation raw pulp of the Junshan Yinzhu tea contains 1.0-5.0mg/ml of protein, 5-15mg/ml of crude polysaccharide, 0.1-0.8mg/ml of total flavone and 1.0-5.0mg/ml of total phenol.
According to one aspect of the invention, the pH value of the Junshan silver needle tea fermentation raw stock is 4.5-6.8.
According to one aspect of the invention, the fermentation is carried out using yeast. Preferably, the yellow wine yeast.
A skin care product comprises the fermentation raw stock of the Junshan silver needle tea.
The fermentation raw pulp of the Junshan silver needle tea is used as skin care toning lotion.
A method for preparing fermentation raw stock of Junshan silver needle tea comprises oven drying folium Camelliae sinensis at 70-80 deg.C, mixing with mechanically ground powder of Junshan silver needle folium Camelliae sinensis, water and fermentation broth to obtain initial system, fermenting to obtain fermentation broth, sterilizing the fermentation broth, and centrifuging to obtain supernatant as fermentation raw stock of Junshan silver needle tea.
According to one aspect of the invention, the mesh number of the Junshan silver needle tea powder is 30-80 meshes, preferably 50 meshes.
According to one aspect of the invention, the kaffir lily tea powder: the proportion of water is (2-10) g (100-300) mL. Preferably, the kaffir lily tea powder: the mass ratio of water is 10g:300 mL.
According to one aspect of the invention, the concentration of the zymogen liquid is 105-108CFU/ml。
According to one aspect of the invention, the ratio relationship between the zymophyte liquid and the Junshan silver needle tea powder is (5-30) ml: (2-20) g. Preferably, the proportion relationship between the zymocyte liquid and the Junshan silver needle tea powder is 5 ml: 10 g.
According to one aspect of the invention, the fermentation temperature of the fermentation is 30-45 ℃, preferably 37 ℃.
According to one aspect of the invention, the fermentation time of the fermentation is 10-50h, preferably 24 h.
According to one aspect of the invention, the centrifugation is carried out at 4000-12000r/min for 15-30 min. Preferably, the centrifugation is performed at 4500r/min for 20 min.
According to one aspect of the invention, the centrifugation radius of the centrifugation is 9-12 cm.
According to one aspect of the invention, the fermentation bacteria are yeasts. Preferably, the fermentation bacteria are yellow wine yeast.
According to one aspect of the invention, the zymophyte is pretreated in advance, and the pretreatment process comprises the following steps:
activating strains: putting the bacterial colony of the zymocyte into a liquid culture medium, and then putting the culture medium into an incubator to activate the strain;
and (3) strain purification: the activated strains are subjected to gradient dilution and plating so as to obtain single colonies; and
expanding culture of strains: inoculating the strain to corresponding liquid culture medium, culturing at 30-45 deg.C in culture box until OD value is 0.5-1.0, and the strain is in log phase, i.e. proper inoculation concentration is 105-108CFU/ml; preferably, the medium is MRS medium.
The fermentation raw juice of the Junshan silver needle tea provided by the invention can be used as an effective component in a skin care product, can also be independently applied as the skin care product, and has an antioxidant function and a whitening function.
The preparation method of the fermentation raw stock by utilizing the Junshan silver needle tea provided by the invention has the following advantages:
(1) the method adopts yellow wine yeast to ferment the Junshan silver needle tea leaves, retains all functional components and activity of the Junshan silver needle tea leaves, and avoids loss of active components caused by an extraction method.
(2) The fermentation method adopted by the invention does not add any organic reagent in the process of extracting the effective components of the Junshan silver needle tea leaves, has mild fermentation temperature and fermentation pH, does not damage the structure of the active components of the plants, and keeps the natural activity of the plants.
(3) After the fermentation is finished in the method, chemical components such as essence and the like are not added into the fermentation raw stock, so that the safety of the product to human bodies is ensured.
(4) The Junshan silver needle tea fermentation raw stock provided by the invention is rich in active substances which have the effects of inhibiting tyrosinase activity and melanin synthesis, so that the Junshan silver needle tea fermentation raw stock has a strong skin whitening function and has a synergistic effect with components in fermentation filtrate.
Drawings
FIG. 1 is a curve of action of Junshan silver needle tea fermentation raw stock on scavenging DPPH free radicals in the embodiment of the invention;
FIG. 2 is a bar graph of the inhibition rate of Junshan silver needle tea fermentation raw pulp on tyrosinase activity and arbutin on tyrosinase activity in the specific embodiment of the invention.
Detailed Description
In the following, only certain exemplary embodiments are briefly described. As those skilled in the art will recognize, the described embodiments may be modified in various different ways, all without departing from the spirit or scope of the present invention. Accordingly, the drawings and description are to be regarded as illustrative in nature, and not as restrictive.
The following disclosure provides many different embodiments or examples for implementing different features of the invention. To simplify the disclosure of the present invention, the components and arrangements of specific examples are described below. Of course, they are merely examples and are not intended to limit the present invention. Furthermore, the present invention may repeat reference numerals and/or letters in the various examples, such repetition is for the purpose of simplicity and clarity and does not in itself dictate a relationship between the various embodiments and/or configurations discussed. In addition, the present invention provides examples of various specific processes and materials, but one of ordinary skill in the art may recognize applications of other processes and/or uses of other materials.
The following description of the preferred embodiments of the present invention is provided for the purpose of illustration and description, and is in no way intended to limit the invention.
The fermentation tubes used in the following examples were yellow wine yeast.
The zymophyte is pretreated in advance, and the pretreatment process comprises the following steps:
activating strains: putting the bacterial colony of the zymocyte into a liquid culture medium, and then putting the culture medium into an incubator to activate the strain;
and (3) strain purification: the activated strains are subjected to gradient dilution and plating so as to obtain single colonies; and
expanding culture of strains: inoculating the strain to corresponding liquid culture medium, culturing at 30-45 deg.C in culture box until OD value is 0.5-1.0, and the strain is in log phase, i.e. proper inoculation concentration is 105-108CFU/ml; preferably, the medium is MRS medium.
The specific processes of strain activation and strain purification are routine technical means for those skilled in the art.
Example 1:
the concentration of zymophyte in the culture medium is 105-1085mL of CFU/mL zymocyte liquid is inoculated into 15g of Junshan silver needle tea powder of 50 meshes and 300mL of water, and cultured in an incubator at 37 ℃ for 24 hours, the obtained fermentation liquid is sterilized at 121 ℃ under high pressure for 15min to inactivate the bacteria, the sterilized fermentation liquid is centrifuged for 20min at 4500r/min and the centrifugation radius of 9cm, and the supernatant is collected, thus obtaining the Junshan silver needle tea fermentation protoplasm cosmetic provided by the invention.
The appearance of the Junshan silver needle tea fermentation raw stock cosmetic prepared in the embodiment is viscous liquid, and the color is colorless, transparent and brown. The pH value is 4.5-6.8, the viscosity is 100-800cP, the content of soluble solid is 1.0-5.0%, the total number of colonies is less than 50CFU/ml, and no pathogenic bacteria are detected. Meets the quality requirement of cosmetics.
The components of the Junshan silver needle tea fermentation raw stock cosmetic are analyzed: the Junshan silver needle tea fermentation raw stock cosmetic prepared by the invention contains 1.0-5.0mg/ml of protein, 5-15mg/ml of crude polysaccharide, 0.1-0.8mg/ml of total flavone and 1.0-5.0mg/ml of total phenol.
Analyzing the antioxidant effect of the fermentation raw stock of the Junshan Yinzhu tea:
the experimental method comprises the following steps:
the positive control is dissolved and diluted by 95% ethanol to form: the test system was verified by using a series of concentration gradients of 0.08mg/mL, 0.04mg/mL, 0.02mg/mL, and 0.01 mg/mL.
Treating a test object: the water-soluble test substance is diluted into a multi-concentration sample by water, and the oil-soluble test substance is diluted into a multi-concentration sample by 95% ethanol.
With reference to Table 1, use10mL, the test tube is provided with a sample tube (T) and a sample background (T)0) DPPH tube (C) and solvent background (C)0) For each sample, 3 parallel tubes are required for each sample tube (T) of each concentration to be tested.
In the sample tube (T) and sample background (T)0) 1mL of the same concentration of each sample solution was added.
In all test tubes (T, T)0、C、C0) Supplementing solvent, adding water to the water-soluble sample, adding 95% ethanol to the oil-soluble sample to make up for 3mL, and mixing.
1mL of DPPH ethanol solution, sample background (T0) and solvent background (C0) were added to sample tube (T) and DPPH tube (C)
Replace with 95% ethanol, shake gently, and stand at room temperature for 5 minutes.
Each reaction solution was transferred to a 1cm cuvette and absorbance was measured at 517 nm.
Table 1: sample liquid adding requirement meter
Analyzing the whitening efficacy of the Junshan Yinzi tea fermented raw juice:
tyrosinase is a key enzyme in melanogenesis, which controls the process of melanogenesis, and its degree of activity plays a major role in pigment deposition. Many whitening and freckle-removing products sold in the market at present achieve the whitening effect by inhibiting tyrosinase, so the strength of the tyrosinase inhibition effect is a main index for evaluating whitening cosmetics.
The whitening function of the sample is evaluated by measuring the influence of the sample on tyrosinase, and the specific method comprises the following steps:
prepare the solution as in table 2:
TABLE 2 solution preparation List
| Unit (mL) | C1 | C2 | T1 | T2 |
| L-tyrosine | 2 | 2 | 2 | 2 |
| Sample (I) | 0 | 0 | 2 | 2 |
| PBS | 4 | 5 | 2 | 3 |
| Tyrosinase enzyme | 1 | 0 | 1 | 0 |
| Total volume | 7 | 7 | 7 | 7 |
Note: c1And T1Adding 1mL of tyrosinase, and enabling the enzyme activity to be 100U/mL.
(1)C2After the tube is prepared and shaken up, the tube is heated in a water bath kettle at 37 ℃ for 10min, and the zero setting is carried out under the wavelength of 475 nm.
(2)C1Mixing the tube solution, shaking, water-bathing at 37 deg.C for 10min, adding tyrosinase 1ml, continuing water-bathing for 10min, and determining C1An absorbance value.
(3) In the same manner as in (1) and (2), with T2Zero setting determination of T1An absorbance value.
(4) The inhibition rate T (%) of the sample on tyrosinase activity was calculated. T (%) ═ C1-T1)/C1×100％
Experiments prove that the inhibition rate of 30 percent of the Junshan Yinzhu fermentation raw pulp on the tyrosinase activity is 45.50 percent, and the inhibition rate of 1 percent of arbutin on the tyrosinase activity is 75 percent, and the inhibition rate is shown in figure 2. Namely, the whitening effect of the 30% Junshan Yinzhen tea fermented raw juice is equivalent to the effect of 60.6% of 1% arbutin, and the whitening effect is certain.
And (3) evaluating the safety of the Junshan Yinzhu fermentation raw stock:
the patch test is mainly used for detecting the irritation of the final cosmetic product or raw material. The invention carries out a human body closed patch test on the Junshan silver needle tea fermentation protoplasm cosmetic and evaluates the potential skin irritation of the Junshan silver needle tea fermentation protoplasm cosmetic.
Suitable volunteers were selected for 30 persons, and were randomly selected in the age range of 18-60 years. 0.020g to 0.025g of solid or semi-solid sample is weighed into a plaque test device for use. The liquid sample, 0.2mL to 0.025mL, was dropped onto the filter paper sheet, which was then placed in the plaque tester. A blank control is set for each sample and an equal amount of sample solvent, such as distilled water or olive oil, is added to the control chamber.
The test part is selected as the back of a human body, and the spot tester is fixedly attached to the back of the testee by using a non-irritant adhesive tape. The test period lasted 24 h. In order to ensure the accuracy, credibility and scientificity of test results, the volunteers cannot remove the spot tester or make the tested part contact water according to the requirements during the test.
And removing the spot tester after 24h, standing for 30min, waiting for the indentation to disappear, and observing the reaction of the skin. If the test result is negative, the test needs to be observed once more at 24h and 48h after the patch test.
The patch test results are shown in table 3:
TABLE 3 Patch test results
"-" ═ negative reactions;
"±" ═ suspicious reaction: only faint erythema;
"+" ═ weak positive reaction (erythema reaction): erythema, infiltration, edema, and possibly pimples;
"+ +", strong positive reaction (herpes response); erythema, infiltration, edema, pimples, herpes; the reaction may be beyond the test area;
"+ + + +" -very strong positive reaction (fusogenic herpes response); obvious erythema, severe infiltration, edema, and fusional herpes; the reaction goes beyond the test area.
As can be seen from Table 3, the fermentation broth of Junshan Yinzhu tea provided in example 1 produced no more than 1 of grade 2 reactions in the experiment. The Junshan silver needle tea fermentation raw stock is safe and does not bring adverse reactions to human bodies.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A fermentation raw stock of Junshan silver needle tea is characterized by being prepared by fermenting Junshan silver needle tea after drying at 70-80 ℃.
2. The Junshan Yinzhen tea fermentation raw stock as claimed in claim 1, wherein the Junshan Yinzhen tea fermentation raw stock contains 1.0-5.0mg/ml of protein, 5-15mg/ml of crude polysaccharide, 0.1-0.8mg/ml of total flavonoids, and 1.0-5.0mg/ml of total phenols;
preferably, the pH value of the Junshan Yinzhu fermentation raw stock is 4.5-6.8.
3. The fermentation raw stock of the Junshan Yinzhi tea of claim 1, wherein the fermentation is carried out by using yeast;
preferably, yellow wine yeast is adopted for fermentation.
4. A skin care product comprising the Junshan silver needle tea fermentation broth of any one of claims 1 to 3.
5. The use of the fermentation broth of Junshan Yinzhu tea according to any one of claims 1 to 3 as a skin-care toning lotion.
6. A preparation method of Junshan silver needle tea fermentation raw stock is characterized by comprising the following steps: mixing the tea dried at 70-80 deg.C, the tea powder, water and zymocyte liquid after mechanical grinding to obtain initial system, fermenting to obtain fermentation liquid, sterilizing, and centrifuging to obtain supernatant as the fermentation raw stock.
7. The method for preparing the fermentation raw pulp of the Junshan silver needle tea according to claim 6, which is characterized by comprising the following steps: the tea powder is characterized in that the mesh number of the Junshan silver needle tea powder is 30-80 meshes, and the preferred mesh number is 50 meshes;
preferably, the Junshan silver needle tea powder: the proportion of water is (2-10) g (100-300) mL; further preferably, the mixed powder of the kaffir lily tea leaves: the mass ratio of water is 10g:300 mL;
preferably, the concentration of the zymogen liquid is 105-108CFU/ml；
Preferably, the proportion relationship between the zymocyte liquid and the Junshan silver needle tea powder is (5-30) ml: (2-20) g; further preferably, the proportion relationship between the zymocyte liquid and the Junshan silver needle tea powder is 5 ml: 10 g.
8. The method for preparing the fermentation raw pulp of the Junshan silver needle tea according to claim 6, which is characterized by comprising the following steps: the fermentation temperature of the fermentation is 30-45 ℃, and preferably 37 ℃;
preferably, the fermentation time of the fermentation is 10-50h, preferably 24 h.
9. The method for preparing the fermentation raw pulp of the Junshan silver needle tea according to claim 6, which is characterized by comprising the following steps: the centrifugation is carried out for 15-30min at the speed of 4000-12000 r/min;
preferably, the centrifugation is performed at 4500r/min for 20 min;
preferably, the centrifugation radius of the centrifugation is 9-12 cm.
10. The method for preparing the fermentation raw pulp of the Junshan silver needle tea according to claim 6, which is characterized by comprising the following steps: the zymocyte is saccharomycetes;
preferably, the fermentation bacteria are yellow wine yeast;
preferably, the pretreatment process comprises:
activating strains: putting the bacterial colony of the zymocyte into a liquid culture medium, and then putting the culture medium into an incubator to activate the strain;
and (3) strain purification: the activated strains are subjected to gradient dilution and plating so as to obtain single colonies; and
expanding culture of strains: inoculating the strain to corresponding liquid culture medium, culturing at 30-45 deg.C in culture box until OD value is 0.5-1.0, and the strain is in log phase, i.e. proper inoculation concentration is 105-108CFU/ml; preferably, the medium is MRS medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010503551.5A CN111616997A (en) | 2020-06-05 | 2020-06-05 | Junshan honeysuckle tea fermentation raw stock and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010503551.5A CN111616997A (en) | 2020-06-05 | 2020-06-05 | Junshan honeysuckle tea fermentation raw stock and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111616997A true CN111616997A (en) | 2020-09-04 |
Family
ID=72267480
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010503551.5A Pending CN111616997A (en) | 2020-06-05 | 2020-06-05 | Junshan honeysuckle tea fermentation raw stock and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111616997A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113827521A (en) * | 2021-10-18 | 2021-12-24 | 上海应用技术大学 | Preparation method of tea powder fermentation liquor and application of tea powder fermentation liquor in cosmetics |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105250168A (en) * | 2015-09-30 | 2016-01-20 | 上海全丽生物科技有限公司 | High mountain tea fermentation raw stock cosmetic and preparation method and application thereof |
-
2020
- 2020-06-05 CN CN202010503551.5A patent/CN111616997A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105250168A (en) * | 2015-09-30 | 2016-01-20 | 上海全丽生物科技有限公司 | High mountain tea fermentation raw stock cosmetic and preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 张景: "《中国茶文化》", 31 January 2018, 天津科学技术出版社, pages: 210 * |
| 林治: "《茶韵心香》", 31 August 2017, 世界图书出版西安有限公司, pages: 158 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113827521A (en) * | 2021-10-18 | 2021-12-24 | 上海应用技术大学 | Preparation method of tea powder fermentation liquor and application of tea powder fermentation liquor in cosmetics |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111544357B (en) | Centella fermented raw stock and preparation method and application thereof | |
| CN111419752B (en) | Four-substance fermented water and preparation method and application thereof | |
| CN110680774B (en) | Preparation method of jasmine double-bacterium fermented cosmetic and application of product | |
| CN106726949B (en) | Grape seed fermentation raw stock cosmetic and preparation method and application thereof | |
| CN110680781B (en) | White peony root fermented cosmetic and preparation method and application thereof | |
| CN109893487B (en) | Rice rose fermented raw pulp and preparation method and application thereof | |
| CN104257545A (en) | Rhodiola rosea fermentation protoplasm cosmetic and preparation method thereof | |
| CN111249218B (en) | Saussurea involucrate fermentation stock solution and preparation method and application thereof | |
| CN113855603B (en) | Combined fermentation product, skin external preparation containing same, and preparation method and application of combined fermentation product | |
| CN105919889B (en) | A kind of blueness bur proferment pulp cosmetic and the preparation method and application thereof | |
| CN108042433B (en) | Whitening antioxidant plant composition, plant composition fermentation liquor, preparation method and application | |
| CN104257598A (en) | Chinese wolfberry fermented raw stock cosmetic and preparation method thereof | |
| CN115572738A (en) | Dendrobium officinale fermentation liquor with anti-inflammatory, relieving and repairing effects as well as preparation method and application thereof | |
| Lin et al. | Study on the properties of Dendrobium officinale fermentation broth as functional raw material of cosmetics | |
| CN106511180A (en) | Codonopsis pilosula fermented raw stock cosmetic, preparation method thereof and application thereof | |
| CN111494269B (en) | Grape fermentation raw pulp and preparation method and application thereof | |
| CN111616997A (en) | Junshan honeysuckle tea fermentation raw stock and preparation method and application thereof | |
| CN115006297A (en) | Revival grass fermentation extracting solution, preparation method and application | |
| CN113476388A (en) | Bulbus Lilii fermented product for cosmetic and its preparation method | |
| CN111588677B (en) | Barley tremella fermentation raw stock and preparation method and application thereof | |
| CN114588091B (en) | Preparation method of cantaloupe fermentation primary pulp and application of cantaloupe fermentation primary pulp in cosmetics | |
| CN105925617A (en) | Radix pueraiae fermentation protoplasm cosmetic product and preparation method and application thereof | |
| CN111374922B (en) | Rice composite fermentation product and preparation method and application thereof | |
| CN111544352B (en) | Tremella-ganoderma lucidum composite fermentation product, cosmetic composition with anti-aging effect and preparation method and application thereof | |
| CN114788806A (en) | Traditional Chinese medicine composition fermented primary pulp with antioxidant and whitening integrated effects and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200904 |
|
| RJ01 | Rejection of invention patent application after publication |