CN104257598A - Chinese wolfberry fermented raw stock cosmetic and preparation method thereof - Google Patents
Chinese wolfberry fermented raw stock cosmetic and preparation method thereof Download PDFInfo
- Publication number
- CN104257598A CN104257598A CN201410479856.1A CN201410479856A CN104257598A CN 104257598 A CN104257598 A CN 104257598A CN 201410479856 A CN201410479856 A CN 201410479856A CN 104257598 A CN104257598 A CN 104257598A
- Authority
- CN
- China
- Prior art keywords
- chinese wolfberry
- cosmetics
- zymocyte
- wolfberry fruit
- fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a Chinese wolfberry fermented raw stock cosmetic and a preparation method thereof. The active ingredient of the Chinese wolfberry fermented raw stock cosmetic refers to a supernatant prepared by sterilizing and centrifuging the fermentation liquor prepared by fermenting an initial system obtained by mixing Chinese wolfberry powder, water and zymocyte, wherein the zymocyte is Lactobacillus delbrueckii. The Chinese wolfberry fruits are subjected to whole plant fermentation by adopting the Lactobacillus delbrueckii, the total functional components and activities of plants are remained, and loss of active ingredients caused by the extraction method is avoided; and moreover, any organic reagent is not added during preparation, the fermentation temperature and the fermentation pH value are mild, the active ingredients of the plants are not damaged, the natural activity of the plant is maintained, essence and other chemical components are not added into the fermented raw stock, and the product safety on a human body is ensured. The product is enriched in lycium barbarum polysaccharides, medlar flavone and other substances, has a synergistic effect with probiotic fermented products in the fermented filtrate and can obviously resist oxidation and ageing.
Description
Technical field
The invention belongs to cosmetic field, the present invention relates to a kind of fermentation Chinese wolfberry fruit oleo stock cosmetics and preparation method thereof.
Background technology
Fructus Lycii is perennial machaka, Hua Zi, arnotto, flourishing.Its fruit Fructus Lycii sweet in the mouth, property are flat, have and grow kidney and replenishing marrow, nourishing the liver to improve visual acuity and wind action of dispelling, be traditional tonic Chinese medicine, have the multiple biological activity such as defying age, moisturizing.The current city value of Fructus Lycii in cosmetics is not almost also developed, and needs a kind of cosmetics giving full play to the beauty functions of Fructus Lycii of exploitation badly.
Summary of the invention
The object of this invention is to provide a kind of fermentation Chinese wolfberry fruit oleo stock cosmetics and preparation method thereof.
Fermentation Chinese wolfberry fruit oleo stock cosmetics provided by the invention, its effective ingredient is that initial system Chinese wolfberry fruit powder, water and zymocyte are obtained by mixing ferments the fermentation liquor sterilizing obtained, the centrifugal supernatant obtained.
In above-mentioned cosmetics, the order number of Chinese wolfberry fruit powder is 20-50 order, is specially 50 orders;
Described zymocyte is lactobacillus, is specially Deshi Lactobacillus, is more specifically lactobacillus delbruockii subspecies bulgaricus (Lactobacillus delbrueckii subsp.Bulgaricus).
Described zymocyte adds with the form of bacterium liquid;
Described initial system is 10 by 10-40ml zymocyte concentration
6-10
8the zymocyte bacterium liquid of CFU/ml, 3-20g Chinese wolfberry fruit powder and 100-300ml water are obtained by mixing.
It is 10 that described initial system is specially 20ml zymocyte concentration
6-10
8the zymocyte bacterium liquid of CFU/ml, 15g Chinese wolfberry fruit powder and 200ml water are obtained by mixing.
In described fermentation step, temperature is 36-48 DEG C, is specially 45 DEG C; Time is 10-16 hour;
In described centrifugation step, rotating speed is 8000-12000r/min, and centrifugal radius is 9-12cm; Centrifugation time is 10-20min.
The method of preparation provided by the invention described fermentation Chinese wolfberry fruit oleo stock cosmetics, comprises the steps:
Ferment after described Chinese wolfberry fruit powder, water and zymocyte are mixed to get initial system according to proportioning, then gained fermentation liquid is carried out successively sterilizing and centrifugal, collect supernatant, obtain described fermentation Chinese wolfberry fruit oleo stock cosmetics.
In said method, the order number of described Chinese wolfberry fruit powder is 20-50 order, is specially 50 orders;
Described zymocyte is lactobacillus, is specially Deshi Lactobacillus, is more specifically lactobacillus delbruockii subspecies bulgaricus (Lactobacillus delbrueckii subsp.Bulgaricus).
Described zymocyte adds with the form of bacterium liquid;
Described initial system is 10 by 10-40ml zymocyte concentration
6-10
8the zymocyte bacterium liquid of CFU/ml, 3-10g Chinese wolfberry fruit powder and 100-300ml water are obtained by mixing.
It is 10 that described initial system is specially 20ml zymocyte concentration
6-10
8the zymocyte bacterium liquid of CFU/ml, 15g Chinese wolfberry fruit powder and 200ml water are obtained by mixing.
In described fermentation step, temperature is 36-48 DEG C; Time is 10-16 hour;
In described centrifugation step, rotating speed is 8000-12000r/min, and centrifugal radius is 9-12cm; Centrifugation time is 10-20min.
In described sterilization steps, sterilizing methods is autoclaving; Sterilising temp is 115 DEG C, and the time is 20min;
In addition, the fermentation Chinese wolfberry fruit oleo stock cosmetics that the invention described above provides, at the application prepared in cosmetics and the cosmetics being effective ingredient with described fermentation Chinese wolfberry fruit oleo stock cosmetics, also belong to protection scope of the present invention.Wherein, described cosmetics are have the product removing DPPH free radical function.
The invention provides a kind of method utilizing FRUCTUS LYCII fermenting and producing cosmetics.The present invention advantageously (1) the present invention adopts Deshi Lactobacillus to carry out whole plant fermentation to FRUCTUS LYCII, remains whole functional component and the activity thereof of plant, avoids the active component loss that extracting method causes.(2) fermentation method of the present invention, in FRUCTUS LYCII extracts active ingredients process, does not add any organic reagent, and fermentation temperature and fermentation pH gentleness, active components of plants structure is not destroyed, and keeps the natural activity of plant.(3), after cosmetics provided by the invention have fermented, do not add essence etc. to chemical composition in fermentation oleo stock, guarantee the safety of product to human body.(4) material such as lycium barbarum polysaccharide, Fructus Lycii flavone be rich in fermentation Chinese wolfberry fruit oleo stock cosmetics provided by the invention, has synergism with the probiotics fermention product component in ferment filtrate, thus has antioxidation and anti-aging effects significantly.
Accompanying drawing explanation
Fig. 1 is that DPPH free radical result schematic diagram removed by fermentation Chinese wolfberry fruit oleo stock cosmetics;
Detailed description of the invention
Following examples further illustrate content of the present invention, but should not be construed as limitation of the present invention.Without departing from the spirit and substance of the case in the present invention, the amendment do the inventive method, step or condition or replacement, all belong to scope of the present invention.
If do not specialize, the conventional means that technological means used in embodiment is well known to those skilled in the art.
The present invention's FRUCTUS LYCII used, purchased from Kang Pu bio tech ltd, Qinghai, is crushed to 20-50 order.
The present invention's lactobacillus delbruockii subspecies bulgaricus used (Lactobacillus delbrueckii subsp.Bulgaricus) is purchased from China General Microbiological culture presevation administrative center.
The bacterium liquid of following embodiment Deshi Lactobacillus used is in accordance with the following steps and obtains:
First strain activates by sweat, makes it keep higher vitality; Then by bacterium purification, make to use single culture during fermentation; Then spread cultivation and make bacterium amount reproduction.Fermented under the condition adapted to by the mixed liquor of the bacterium access Chinese wolfberry fruit powder after spreading cultivation and water, then centrifuging and taking supernatant detects.Detailed process is as follows:
(1) actication of culture: picking colony puts into fluid medium from the inclined-plane of preservation, by actication of culture.
(2) purifying agaric: by the strain gradient dilution bed board of activation, to obtain single bacterium colony.
(3) strain spreads cultivation: by stand-by strain inoculation to skim milk and water composition culture medium in (defatted milk powder 100g/L, pH nature) in, cultivate in incubator, cultivation temperature is 36-48 DEG C, during to its OD value=0.5-1.0, strain is in exponential phase, is suitable inoculum density, and this concentration is 10
6-10
8cFU/ml.
The preparation of embodiment 1, fermentation Chinese wolfberry fruit oleo stock cosmetics and detection
Be 10 by the concentration spread cultivation in culture medium
6-10
8the Deshi Lactobacillus bacterium liquid 20ml of CFU/ml is inoculated in 50 object Chinese wolfberry fruit powder 15g and 200g water, and in 45 DEG C of incubators, carry out fermentation after 16 hours, gained fermentation liquid is carried out 115 DEG C of autoclavings, 20min, make bacterium inactivation, by the fermentation liquid after sterilizing at 12000r/min, centrifugal radius is 9cm; Centrifugation time is 20min, collects supernatant, is fermentation Chinese wolfberry fruit oleo stock cosmetics provided by the invention.
The outward appearance that this embodiment prepares gained fermentation Chinese wolfberry fruit oleo stock cosmetics is thick liquid, color is that pale red is to peony.PH value is 5.2-6.8, and viscosity is 50-300cP, and the mass percentage that solubility contains thing is admittedly 1.5-5.0%, and total plate count is less than 50CFU/ml, detects without pathogenic bacterium.Meet cosmetics quality requirement.
Carry out component analysis to these fermentation Chinese wolfberry fruit oleo stock cosmetics, protein detection method is with reference to GB5009.5-2010; Crude polysaccharides detection method is with reference to GB/T 5009.8-2008; Aminoacid detection method is with reference to GB/T 5009.124-2003.Acquired results is as follows:
Containing protein 3.6g/kg, crude polysaccharides 0.92g/kg, total flavones 3.90g/kg (in rutin), total phenols 0.130g/kg in these fermentation Chinese wolfberry fruit oleo stock cosmetics.Total amino acids 1.25g/kg, comprising aspartic acid 0.152g/kg, threonine 0.051g/kg, serine 0.092g/kg, glutamic acid 0.240g/kg, glycine 0.098g/kg, alanine 0.072g/kg, valine 0.044g/kg, isoleucine 0.034g/kg, leucine 0.067g/kg, tyrosine 0.079g/kg, histidine 0.055g/kg, arginine 0.083g/kg, phenylalanine 0.132g/kg.
The anti-oxidation efficacy analysis of embodiment 2, fermentation Chinese wolfberry fruit oleo stock cosmetics
DPPH is a kind of organic free radical of early stage synthesis, be commonly used to the hydrogen supply capacity assessing polyphenoils, it is highly stable in organic solvent, in purple, and have a characteristic absorption peak at place, when running into free radical scavenger, the lone pair electrons of DPPH are paired and make it fade, and namely diminish at the light absorption value of maximum absorption wave strong point.Therefore, the change by measuring light absorption value carrys out the elimination effect of assess sample to DPPH free radical.
The specific experiment step of DPPH free radical scavenging experiment is:
(1) liquid to be measured and 2 × 10 of equal-volume (being generally 3mL) is got
-4the DPPH solution mixing (A of mol/L
1pipe);
(2) isopyknic dehydrated alcohol (determinand solvent) and 2 × 10 is got
-4the DPPH solution mixing (A of mol/L
2pipe);
(3) isopyknic dehydrated alcohol is got and liquid to be measured mixes (A
3pipe);
(4), after reacting 30min, under 517nm, A is surveyed
1, A
2, A
3pipe absorbance.
Clearance rate computing formula is: clearance rate (%)=[(A
2+ A
3)-A
1]/A
2
Make fermentation Chinese wolfberry fruit oleo stock to DPPH free radical scavenging effect curves, see accompanying drawing 1.
As seen from the figure, embodiment 1 gained fermentation Chinese wolfberry fruit oleo stock is to the IC50=8.6% of DPPH scavenging action, illustrating that fermentation Chinese wolfberry fruit oleo stock has stronger oxidation resistance can scavenging free radicals, promote cellular metabolism, strengthen cell viability, improve the 26S Proteasome Structure and Function of body, improve body vitality, thus delay cell senescence, play its antidotal effect.
The safety evaluatio of embodiment 3, fermentation Chinese wolfberry fruit oleo stock
Patch test is mainly used for the zest detecting cosmetics finished product or raw material.The present invention carries out human closed formula patch test to fermentation Chinese wolfberry fruit oleo stock cosmetics, assesses its potential skin irritation.
Select suitable volunteer 30 people, the range of age is at 18-60 year Stochastic choice.Take 0.020g-0.025g solid sample or semisolid sample are put into speckle examination device for subsequent use.Fluid sample 0.2mL to 0.025mL is dripped on filter paper, then filter paper is placed in speckle examination device.Each sample all arranges blank, in contrast speckle examination device hole, add the sample solvent with sample equivalent, as distilled water or olive oil.
Human body back is elected at test position as, utilizes non-irritating adhesive tape speckle to be tried device and fixedly sticks in experimenter back.Test period continues 24h.In order to the accurate, credible of result of the test and science, test period volunteer as requested, speckle examination device can not be removed, recipient site also can not be made to contact water.Remove speckle examination device after 24h, after leaving standstill 30min, wait for that impression disappears, observe the reaction of skin.If result of the test is negative, then 24h and 48h after patch test is needed to observe once respectively again.
Patch test results is as table 1:
Table 1, patch test results
* "-"=negative reaction;
" ± "=suspicious reaction: only have faint erythema;
"+"=weak positive reaction (erythematous response): erythema, infiltration, edema, can pimple be had;
" ++ "=strong positive reaction (herpes reaction); Erythema, infiltration, edema, pimple, herpes; Reaction can exceed tested district;
" +++ "=extremely strong positive reaction (reaction of amalgamation herpes); Obvious erythema, severe infiltration, edema, amalgamation herpes; Reaction exceeds tested district.
As shown in Table 1, the fermentation Chinese wolfberry fruit oleo stock cosmetics that embodiment 1 provides all produce in test suspicious reaction only 1 example.Through judging, fermentation Chinese wolfberry fruit oleo stock cosmetics provided by the invention have safety, can not bring untoward reaction to human body.
Claims (10)
1. fermentation Chinese wolfberry fruit oleo stock cosmetics, its effective ingredient is that initial system Chinese wolfberry fruit powder, water and zymocyte are obtained by mixing ferments the fermentation liquor sterilizing obtained, the centrifugal supernatant obtained;
Zymocyte concentration in described initial system is 10
6-10
8cfu/ml.
2. cosmetics according to claim 1, is characterized in that: the order number of described Chinese wolfberry fruit powder is 20-50 order, is specially 50 orders;
Described zymocyte is lactobacillus, is specially Deshi Lactobacillus, is more specifically lactobacillus delbruockii subspecies bulgaricus (Lactobacillus delbrueckii subsp.Bulgaricus).
3. cosmetics according to claim 1 and 2, is characterized in that: described zymocyte adds with the form of bacterium liquid;
Described initial system is 10 by 10-40ml zymocyte concentration
6-10
8the zymocyte bacterium liquid of cfu/ml, 3-20g Chinese wolfberry fruit powder and 100-300ml water are obtained by mixing.
4., according to the arbitrary described cosmetics of claim 1-3, it is characterized in that: in described fermentation step, temperature is 36-48 DEG C; Time is 10-16 hour;
In described centrifugation step, rotating speed is 8000-12000r/min, and centrifugal radius is 9-12cm; Centrifugation time is 10-20min.
5. prepare a method for the arbitrary described fermentation Chinese wolfberry fruit oleo stock cosmetics of claim 1-4, comprise the steps:
Ferment after described Chinese wolfberry fruit powder, water and zymocyte are mixed to get initial system according to proportioning, then gained fermentation liquid is carried out successively sterilizing and centrifugal, collect supernatant, obtain described fermentation Chinese wolfberry fruit oleo stock cosmetics.
6. method according to claim 5, is characterized in that: the order number of described Chinese wolfberry fruit powder is 20-50 order, is specially 50 orders;
Described zymocyte is lactobacillus, is specially Deshi Lactobacillus, is more specifically lactobacillus delbruockii subspecies bulgaricus (Lactobacillus delbrueckii subsp.Bulgaricus).
7. the method according to claim 5 or 6, is characterized in that: described zymocyte adds with the form of bacterium liquid;
Described initial system is 10 by 10-40ml zymocyte concentration
6-10
8the zymocyte bacterium liquid of cfu/ml, 3-10g Chinese wolfberry fruit powder and 100-300ml water are obtained by mixing.
8., according to the arbitrary described method of claim 5-7, it is characterized in that: in described fermentation step, temperature is 36-48 DEG C; Time is 10-16 hour;
In described centrifugation step, rotating speed is 8000-12000r/min, and centrifugal radius is 9-12cm; Centrifugation time is 10-20min.
9. the application in cosmetics prepared by the arbitrary described fermentation Chinese wolfberry fruit oleo stock cosmetics of claim 1-4;
With the cosmetics that the arbitrary described fermentation Chinese wolfberry fruit oleo stock cosmetics of claim 1-4 are effective ingredient.
10. application according to claim 9 or cosmetics, is characterized in that: described cosmetics are have the product removing DPPH free radical function.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410479856.1A CN104257598A (en) | 2014-09-18 | 2014-09-18 | Chinese wolfberry fermented raw stock cosmetic and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410479856.1A CN104257598A (en) | 2014-09-18 | 2014-09-18 | Chinese wolfberry fermented raw stock cosmetic and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104257598A true CN104257598A (en) | 2015-01-07 |
Family
ID=52149262
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410479856.1A Pending CN104257598A (en) | 2014-09-18 | 2014-09-18 | Chinese wolfberry fermented raw stock cosmetic and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104257598A (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105147585A (en) * | 2015-09-30 | 2015-12-16 | 上海全丽生物科技有限公司 | Lavender fermented puree and preparation method and application thereof |
| CN105902468A (en) * | 2016-05-20 | 2016-08-31 | 秦瑜 | Whitening anti-aging facial cream |
| CN106511180A (en) * | 2016-12-22 | 2017-03-22 | 北京工商大学 | Codonopsis pilosula fermented raw stock cosmetic, preparation method thereof and application thereof |
| CN108309922A (en) * | 2018-03-12 | 2018-07-24 | 上海应用技术大学 | One kind three spends proferment pulp cosmetic and preparation method thereof |
| CN108338946A (en) * | 2018-03-12 | 2018-07-31 | 黑龙江军门现代农业发展有限公司 | A kind of beauty mask and preparation method thereof containing silk peptide |
| CN108815031A (en) * | 2018-07-16 | 2018-11-16 | 广东卓明仕生物科技有限公司 | A kind of Chinese medicine composition fermentation magma and its preparation method and application |
| CN109125164A (en) * | 2018-08-22 | 2019-01-04 | 上海仪玳化妆品有限公司 | A kind of lycium ruthenicum fermentation magma and preparation method thereof |
| CN109288750A (en) * | 2018-10-31 | 2019-02-01 | 浙江大学华南工业技术研究院 | A kind of lycium ruthenicum fermentation liquid and preparation method thereof and the application in cosmetics |
| CN111109604A (en) * | 2020-03-11 | 2020-05-08 | 深圳市清生元生物技术股份有限公司 | A fructus Lycii fermented product with antioxidant activity and its preparation method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060165636A1 (en) * | 2003-03-10 | 2006-07-27 | Kouhei Hasebe | Hair treatment composition and hair cosmetic for damaged hair |
| CN1839793A (en) * | 2006-01-17 | 2006-10-04 | 高铎 | Beauty and health cosmetics |
| CN103027860A (en) * | 2011-09-29 | 2013-04-10 | 庞来祥 | Wolfberry nourishing sleeping mask and preparation method thereof |
| CN103704557A (en) * | 2013-12-30 | 2014-04-09 | 徐州绿之野生物食品有限公司 | Preparation method and application of lycium barbarum fermented and concentrated juice |
-
2014
- 2014-09-18 CN CN201410479856.1A patent/CN104257598A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060165636A1 (en) * | 2003-03-10 | 2006-07-27 | Kouhei Hasebe | Hair treatment composition and hair cosmetic for damaged hair |
| CN1839793A (en) * | 2006-01-17 | 2006-10-04 | 高铎 | Beauty and health cosmetics |
| CN103027860A (en) * | 2011-09-29 | 2013-04-10 | 庞来祥 | Wolfberry nourishing sleeping mask and preparation method thereof |
| CN103704557A (en) * | 2013-12-30 | 2014-04-09 | 徐州绿之野生物食品有限公司 | Preparation method and application of lycium barbarum fermented and concentrated juice |
Non-Patent Citations (2)
| Title |
|---|
| 张春祥等: "枸杞乳酸发酵和酒精发酵前后各种成分的变化", 《湖北农业科学》 * |
| 王建新: "《化妆品植物原料大全》", 30 June 2012, 中国纺织出版社 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105147585A (en) * | 2015-09-30 | 2015-12-16 | 上海全丽生物科技有限公司 | Lavender fermented puree and preparation method and application thereof |
| CN105902468A (en) * | 2016-05-20 | 2016-08-31 | 秦瑜 | Whitening anti-aging facial cream |
| CN106511180A (en) * | 2016-12-22 | 2017-03-22 | 北京工商大学 | Codonopsis pilosula fermented raw stock cosmetic, preparation method thereof and application thereof |
| CN108309922A (en) * | 2018-03-12 | 2018-07-24 | 上海应用技术大学 | One kind three spends proferment pulp cosmetic and preparation method thereof |
| CN108338946A (en) * | 2018-03-12 | 2018-07-31 | 黑龙江军门现代农业发展有限公司 | A kind of beauty mask and preparation method thereof containing silk peptide |
| CN108815031A (en) * | 2018-07-16 | 2018-11-16 | 广东卓明仕生物科技有限公司 | A kind of Chinese medicine composition fermentation magma and its preparation method and application |
| CN108815031B (en) * | 2018-07-16 | 2021-04-13 | 广东卓明仕生物科技有限公司 | Traditional Chinese medicine composition fermented raw pulp with function of balancing skin micro-ecology and preparation method and application thereof |
| CN109125164A (en) * | 2018-08-22 | 2019-01-04 | 上海仪玳化妆品有限公司 | A kind of lycium ruthenicum fermentation magma and preparation method thereof |
| CN109288750A (en) * | 2018-10-31 | 2019-02-01 | 浙江大学华南工业技术研究院 | A kind of lycium ruthenicum fermentation liquid and preparation method thereof and the application in cosmetics |
| CN111109604A (en) * | 2020-03-11 | 2020-05-08 | 深圳市清生元生物技术股份有限公司 | A fructus Lycii fermented product with antioxidant activity and its preparation method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104257598A (en) | Chinese wolfberry fermented raw stock cosmetic and preparation method thereof | |
| CN104257545B (en) | A kind of Radix Rhodiolae proferment pulp cosmetic and preparation method thereof | |
| CN105193658B (en) | A kind of rice proferment pulp cosmetic and the preparation method and application thereof | |
| CN105919889B (en) | A kind of blueness bur proferment pulp cosmetic and the preparation method and application thereof | |
| CN110680774B (en) | Preparation method of jasmine double-bacterium fermented cosmetic and application of product | |
| CN105002254A (en) | Preparation method and application of tremella fermentation extract | |
| CN111249218B (en) | Saussurea involucrate fermentation stock solution and preparation method and application thereof | |
| CN102727412B (en) | Method for preparing macadimia nut green-peel extractum and lotion | |
| CN105726398B (en) | A kind of tremella prickly pear ferment face cream and preparation method thereof | |
| CN111407678A (en) | Microemulsion with moisturizing, skin-brightening and anti-aging effects and preparation method thereof | |
| CN106726949B (en) | Grape seed fermentation raw stock cosmetic and preparation method and application thereof | |
| CN105147587A (en) | Ganoderma lucidum fermentation protoplasm cosmetic and preparation method and application thereof | |
| CN106309309A (en) | Milk fermented product filtrate as well as preparation method and application thereof | |
| CN113520942B (en) | Anti-oxidation and anti-aging five-ginseng fermentation product and preparation method and application thereof | |
| CN105147588A (en) | Ginseng fermentation puree as well as preparation method and application thereof | |
| CN103800247B (en) | Thick polysaccharide face cream of one main laver and preparation method thereof | |
| KR20150039263A (en) | Cosmetic composition containing fermented herb extracts and marine derived materials | |
| CN115125153B (en) | Preparation method and application of galactose yeast-like bacteria fermentation product filtrate | |
| CN105769601B (en) | Mud mask and preparation method thereof is nourished in a kind of walnut ferment whitening | |
| CN106511180A (en) | Codonopsis pilosula fermented raw stock cosmetic, preparation method thereof and application thereof | |
| CN115554226A (en) | Multi-strain fermentation filtrate and preparation method and application thereof | |
| CN106580789A (en) | Radix salviae miltiorrhizae fermented primary pulp cosmetic product as well as preparation method and applications thereof | |
| CN105147564A (en) | Purslane fermentation protoplasm ceramic and preparation method and application | |
| CN115006297A (en) | Revival grass fermentation extracting solution, preparation method and application | |
| CN108743476A (en) | A kind of composite plant fermentation composition with red blood silk and preparation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150107 |
|
| RJ01 | Rejection of invention patent application after publication |