CN106580789A - Radix salviae miltiorrhizae fermented primary pulp cosmetic product as well as preparation method and applications thereof - Google Patents

Radix salviae miltiorrhizae fermented primary pulp cosmetic product as well as preparation method and applications thereof Download PDF

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Publication number
CN106580789A
CN106580789A CN201611196952.0A CN201611196952A CN106580789A CN 106580789 A CN106580789 A CN 106580789A CN 201611196952 A CN201611196952 A CN 201611196952A CN 106580789 A CN106580789 A CN 106580789A
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CN
China
Prior art keywords
radix salviae
salviae miltiorrhizae
preparation
fermentation
primary pulp
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CN201611196952.0A
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Chinese (zh)
Inventor
王昌涛
李萌
方祥铭
方晓薇
赵丹
王冬冬
史豆豆
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Shanghai Biotruly Biotechnology Co Ltd
Beijing Technology and Business University
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Shanghai Biotruly Biotechnology Co Ltd
Beijing Technology and Business University
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Application filed by Shanghai Biotruly Biotechnology Co Ltd, Beijing Technology and Business University filed Critical Shanghai Biotruly Biotechnology Co Ltd
Priority to CN201611196952.0A priority Critical patent/CN106580789A/en
Publication of CN106580789A publication Critical patent/CN106580789A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/08Anti-ageing preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/70Biological properties of the composition as a whole
    • A61K2800/72Hypo-allergenic
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

Abstract

The invention provides a radix salviae miltiorrhizae fermented primary pulp cosmetic product as well as a preparation method and applications of the radix salviae miltiorrhizae fermented primary pulp cosmetic product. The preparation method of radix salviae miltiorrhizae fermented primary pulp comprises the following steps: mixing radix salviae miltiorrhizae dry powder, water and bacterial liquid, thus obtaining an original system, and carrying out fermentation culturing on the original system, thus obtaining the radix salviae miltiorrhizae fermented primary pulp, wherein in the bacterial liquid, the bacterial strain is lactococcus lactis. The applications of the radix salviae miltiorrhizae fermented primary pulp in preparing cosmetic products with at least one of the four following functions or the applications of the radix salviae miltiorrhizae fermented primary pulp directly taken as the cosmetic products with at least one of the four following functions are within the protection scope of the invention: (1) scavenging DPPH free radicals; (2) scavenging hydroxyl free radicals; (3) resisting oxidation; and (4) resisting aging. The cosmetic product is a facial mask, essence or a toner. According to the method provided by the invention, the active substances are extracted from radix salviae miltiorrhizae by adopting a fermentation engineering technology, the operation is simple, since no foreign materials are added, the prepared radix salviae miltiorrhizae fermented product, namely, the radix salviae miltiorrhizae fermented primary pulp is safe and harmless to the skin, and has good anti-aging activity.

Description

A kind of Radix Salviae Miltiorrhizae proferment pulp cosmetic and preparation method and application
Technical field
The invention belongs to biological technical field, and in particular to a kind of Radix Salviae Miltiorrhizae proferment pulp cosmetic and preparation method thereof with should With.
Background technology
Radix Salviae Miltiorrhizae is dried root and rhizome for Lamiaceae Salvia plant Radix Salviae Miltiorrhizae Salvia miltiorrhiza Bunge's, is One of the most frequently used blood-activating and stasis-removing, head is loaded in《Sheng Nong's herbal classic》, it is listed in careless portion's top grade.Radix Salviae Miltiorrhizae, bitter in the mouth, cold nature, Enter the heart, Liver Channel, have functions that promoting blood flow to regulate menstruation, stasis-dispelling and pain-killing, cool blood to disappear carbuncle, relieving restlessness that clears away heart-fire, nourishing blood to tranquillize the mind.Gu has " red simply The saying of ginseng, same four things of work(":Enrich blood hemopoietic, merits and demerits return ground;Blood is adjusted to hold back blood, power bears Radix Paeoniae;Removing blood stasis tissue regeneration promoting, property times Rhizoma Chuanxiong.Radix Salviae Miltiorrhizae The main purpose that blood circulation promoting and blood stasis dispelling is reached by the effect of " blood-nourishing ".Radix Salviae Miltiorrhizae, as traditional blood-activating and stasis-removing, is also modern One of main Chinese medicine of medical research.Its main chemical compositions is fat-soluble diterpene quinone and water miscible phenolic acids Compound.Research in recent years finds that Radix Salviae Miltiorrhizae has improving the aspects such as cerebral ischemia reperfusion injury, hemorheology and platelet function Pharmacologically active.
The safety of active components of plants Yin Qigao and functional activity, have significantly penetrated into food, medicine, cosmetics etc. Industry.Therefore, extracted from plant using various extractive techniques and have become current study hotspot with highly active composition. Traditional extractive technique includes organic solvent extraction, hot water extraction method, system solvent extraction method etc., but these method product peaces It is complete that property is low, time-consuming, extraction ratio is low.The high and environmentally friendly Novel extraction method of safe, extraction ratio increasingly enjoys people Concern.It is the various enzymes secreted in growth course using microorganism that microbial method extracts active components of plants, so as to plant In active component extracted and modified.
The content of the invention
The preparation method of oleo stock it is an object of the invention to provide a kind of Radix Salviae Miltiorrhizae ferments.
The preparation method of Radix Salviae Miltiorrhizae fermentation oleo stock provided by the present invention, comprises the steps:By Radix Salviae Miltiorrhizae dry powder, water and bacterium solution Initial system is mixed to get, fermentation culture is carried out to the initial system, obtain Radix Salviae Miltiorrhizae fermentation oleo stock.
In above-mentioned preparation method, the Radix Salviae Miltiorrhizae dry powder is obtained from the root of Radix Salviae Miltiorrhizae by pulverizer grinding and sieving, and particle diameter can For 30~40 mesh, concretely 30 mesh.
In the bacterium solution, strain is lactococcus lactis, concretely lactococcus lactis Lactococus lacti, and ATCC is compiled Number be 19435.
The bacterial concentration can be (105~108)CFU/mL。
The pH value of the bacterium solution can be 6.5~7.5, concretely 7.0
In the initial system, the proportioning of the bacterium solution, Radix Salviae Miltiorrhizae dry powder and water can be (5~10mL):(18~22) g: 280mL, concretely 8mL:20g:300mL.
The temperature of the fermentation culture can be 35~45 DEG C, concretely 37 DEG C;Time can be 45~55h, concretely 48h。
Above-mentioned preparation method also includes that the fermentation liquid to obtaining after the fermentation culture is sterilized successively, is centrifuged, being taken The step of clear liquid obtains Radix Salviae Miltiorrhizae fermentation oleo stock.
The condition of the sterilizing is as follows:Sterilising temp can be 115~121 DEG C, concretely 115 DEG C;Sterilization time can be 25~35min, concretely 30min.
The condition of the centrifugation is as follows:Centrifugal rotational speed can be 3800~4200r/min, concretely 4000r/min;Centrifugation Time can be 10~15min, concretely 10min;Centrifugation radius can be 9cm.
The Radix Salviae Miltiorrhizae fermentation oleo stock that said method is prepared falls within protection scope of the present invention.
Above-mentioned Radix Salviae Miltiorrhizae fermentation oleo stock is being prepared with following 1) -4) at least one of application in the cosmetics of function or Directly as with following 1) -4) at least one of application in the cosmetics of function, also within the scope of the present invention:
1) DPPH free radicals are removed;
2) scavenging hydroxyl;
3) antioxidation;
4) defying age.
In above-mentioned application, the cosmetics are included but is not limited to:Facial film, essence or cosmetic water.
The inventive method extracts active substance using fermentation engineering from Radix Salviae Miltiorrhizae, simple to operate, and due to without Any foreign substance, the Radix Salviae Miltiorrhizae fermented product for preparing i.e. Radix Salviae Miltiorrhizae fermentation oleo stock resists to skin safe fanout free region with higher Aging activity.
The present invention has the advantages that:
(1) preparation method of the Radix Salviae Miltiorrhizae fermented product (i.e. Radix Salviae Miltiorrhizae fermentation oleo stock) that the present invention is provided adopts fermentation engineering, By selecting suitable strain and fermentation condition, extract from Radix Salviae Miltiorrhizae and obtain active substance.On the one hand, plant can at utmost be retained Active component in thing, and relative to traditional water extraction, it is to avoid the introducing of organic solvent, safety and environmental protection;On the other hand, no Need to add the foreign substance such as enzyme, not only save production cost, and it is maximized simplify production stage, make the fermentation technique A large amount of productions, industrialized production can be realized, and the stability of product quality can be fully ensured that.
(2) it is natural that the Radix Salviae Miltiorrhizae fermentation oleo stock that the present invention is provided contains the indispensable aminoacid of healthy skin, mineral etc. Composition, and due to being not introduced into foreign substance in preparation process, without any chemical composition, can be directly as facial film or elite The finished product of liquid or cosmetic water is used, more natural than existing other products on the market, will not cause any negative interaction to skin.
(3) the Radix Salviae Miltiorrhizae fermentation oleo stock that the present invention is provided, with stronger oxidation resistance DPPH free radicals and hydroxyl can be removed Free radical, improves body vitality, so as to delay cell senescence, has functions that defying age.
Description of the drawings
Fig. 1 is relation curve of the Radix Salviae Miltiorrhizae fermentation oleo stock to DPPH free radical scavenging activities.
Fig. 2 is relation curve of the Radix Salviae Miltiorrhizae fermentation oleo stock to Scavenging action to hydroxyl free radical.
Specific embodiment
Experimental technique used in following embodiments if no special instructions, is conventional method.
Material used, reagent etc. in following embodiments, if no special instructions, commercially obtain.
Fluid medium in following embodiments is made up of solute and solvent, and solvent is water, solute and its in liquid culture Concentration in base is:Glucose 20g/L, peptone 20g/L, yeast extract 10g/L, pH value is 7.0.
YPD culture medium in following embodiments is made up of solute and solvent, and solvent is water, solute and its liquid medium within In concentration be:Yeast extract 1% (mass fraction), tryptone 2% (mass fraction), glucose 2% (mass fraction), PH value is 7.0.
Radix Salviae Miltiorrhizae dry powder (from the root of Radix Salviae Miltiorrhizae) in following embodiments is sold from market, 30 mesh.
Lactococcus lactis Lactococus lacti in following embodiments, ATCC numbering is 19435, can be bought from ATCC Obtain.
Radix Salviae Miltiorrhizae bacterium solution in following embodiments is obtained in accordance with the following steps:
1st, the activation of strain
The ring of picking lactococcus lactis Lactococus lacti bacterium colonies one is put into strain in shaking table in fluid medium Activation, the bacterium solution after being activated.
2nd, the purification of strain
Bacterium solution gradient dilution bed board after the activation that step 1 is obtained, to obtain single bacterium colony, obtains lactic acid breast after purification Coccus.
3rd, the amplification culture of strain
The strain after purification that step 2 is obtained is inoculated in YPD culture medium (pH value is 7.0), in 25 DEG C of shaking table Culture, as OD values=0.5-1.0, (strain is in logarithmic (log) phase, and concentration is 10 to obtain zymocyte bacterium solution5~108CFU/mL)。
Embodiment 1, prepare Radix Salviae Miltiorrhizae fermentation oleo stock
Radix Salviae Miltiorrhizae fermentation oleo stock is prepared in accordance with the following steps:
(1) concentration for obtaining 8mL steps 3 is 105~108The zymocyte bacterium solution of CFU/mL is inoculated into the Radix Salviae Miltiorrhizae dry powder of 20g In the water of 300mL, fermentation system is obtained;
(2) above-mentioned fermentation system is fermented 48 hours in 37 DEG C of shaking tables, obtains tunning;
(3) by the 30min that sterilizes at 115 DEG C of above-mentioned tunning, inactivate bacterium, the tunning after being sterilized;To go out Tunning after bacterium is that 10min is centrifuged under conditions of 9cm in 4000r/min, centrifugation radius, abandons precipitation, collects supernatant, i.e., For Radix Salviae Miltiorrhizae fermentation oleo stock.
Application of embodiment 2, the Radix Salviae Miltiorrhizae fermentation oleo stock in as cosmetics
First, the property of Radix Salviae Miltiorrhizae fermentation oleo stock
By the fermentation oleo stock Jing activated carbon decolorizing of the prepared gained of embodiment 1, de-taste.Its outward appearance is thick liquid, color For faint yellow to brown color.PH value 6.1-6.8, viscosity 190-230cP, solubility Solid content content 3.0-3.5%, total plate count Less than 50CFU/mL, without pathogenic bacterium detection.According to hygienic standards for cosmetics GB7916-87, cosmetics total number of bacteria is not higher than 1000CFU/ml, so this fermentation oleo stock meets cosmetics quality requirement.
Component analyses are carried out to the Radix Salviae Miltiorrhizae fermentation oleo stock, crude polysaccharides detection method is with reference to GB/T 5009.8-2008;Flavone , with reference to GB/T 5009.124-2003, protein detection method is with reference to GB5009.5-2010 for detection method;Total phenols detection method is joined It is as follows according to GB/T 8313-2008 acquired results:
Obtained Radix Salviae Miltiorrhizae fermentation oleo stock 16.61g/kg containing crude polysaccharides of the invention, total flavones 0.51g/kg, total phenols 0.37g/ Kg, total protein 7.50g/kg.
2nd, the safety detection of Radix Salviae Miltiorrhizae fermentation oleo stock
Human body patch test is mainly used for detecting the zest of cosmetics finished product or raw material.The present invention is to embodiment 1 The Radix Salviae Miltiorrhizae fermentation oleo stock of middle acquisition carries out human closed formula patch test, it is intended to which its potential skin irritation is estimated.
1st, subjects
The people of suitable volunteer 30 is selected, the range of age is in 18-60 year random selection.
2nd, test method
Weigh 0.020g-0.025g solid samples or semisolid sample is put into speckle and tries standby in device.By fluid sample 0.2mL to 0.025mL Deca is on filter paper, then filter paper is placed in speckle examination device.Each sample is respectively provided with blank, The sample solvent with sample equivalent, such as distilled water or olive oil are added in control speckle examination device hole (the present embodiment adopts distilled water).
Human body back is elected at test position as, is fixed speckle examination device using nonirritant adhesive tape and is pasted on experimenter back. Test period continues 24h.For accurate, the credible and science of result of the test, volunteer is as requested during testing, it is impossible to pluck Fall speckle examination device, can not also make recipient site contact water.Speckle examination device is removed after 24h, after standing 30min, waits impression to disappear, observation The reaction of skin.If result of the test is feminine gender, 24h and 48h after patch test is needed to observe again once respectively.
3rd, result of the test
As shown in table 1, the implication that each symbol is represented in table 1 is as follows for patch test results:"-"=negative reaction;" ± "= Suspicious reaction:Only faint erythema;"+"=weakly positive reaction (erythematous response):Erythema, infiltration, edema, there can be pimple;"++" =strong positive reaction (herpess reaction);Erythema, infiltration, edema, pimple, herpess;Reaction can exceed tested area;" +++ "=it is extremely strong Positive reaction (reaction of amalgamation herpess);Obvious erythema, severe infiltration, edema, amalgamation herpess;Reaction exceeds tested area.
Criterion:According to the number for occurring 1 grade of skin adverse reaction in 2007,30 experimenters of cosmetics health specification More than 5, or the number for two grades of skin adverse reactions occur is more than 2, or any 1 three-level of appearance or three-level above skin are not During good reaction, then judge that tested material has untoward reaction to human body, conversely, be then considered as being had no adverse reaction to human body.
The patch test results of the Radix Salviae Miltiorrhizae fermentation oleo stock that table 1, embodiment 1 are obtained
As can be seen from Table 1:The Radix Salviae Miltiorrhizae fermentation oleo stock that embodiment 1 is obtained produces 1 suspicious reaction, illustrates that the present invention is carried For Radix Salviae Miltiorrhizae fermentation oleo stock be respectively provided with safety, body will not be given people and bring untoward reaction.
3rd, the antioxygenic property detection of Radix Salviae Miltiorrhizae fermentation oleo stock
DPPH is a kind of organic free radical of early stage synthesis, is commonly used to assess the hydrogen supply capacity of polyphenoils, and it is organic It is highly stable in solvent, in purple, and there are a characteristic absorption peak, when free radical scavenger is run into, DPPH at 517nm Lone pair electrons be paired and make it fade, that is, diminish in the light absorption value of maximum absorption wave strong point.Therefore, can be by determining The change of light absorption value is evaluating elimination effect of the sample to DPPH free radicals.
Take the Radix Salviae Miltiorrhizae fermentation oleo stock that different amounts of embodiment 1 prepares to be dissolved in deionized water, volume basis are obtained dense Spend a series of Radix Salviae Miltiorrhizaes fermentation oleo stock prepare liquid for 0.63%, 1.25%, 2.5%, 5% and 10%.
DPPH free radical scavengings experiment specific experiment step be:
(1) prepare liquid and 2 × 10 of equal-volume (generally 3mL) is taken-4The DPPH solution of mol/L mixes (A1Pipe);
(2) isopyknic dehydrated alcohol (determinand solvent) and 2 × 10 is taken-4The DPPH solution of mol/L mixes (A2Pipe);
(3) take isopyknic dehydrated alcohol and mix (A with prepare liquid3Pipe);
(4) react after 30min, under 517nm A is surveyed1、A2、A3Pipe absorbance.
Clearance rate computing formula is:Clearance rate (%)=[(A2+A3)-A1]/A2 (1)
As abscissa, clearance rate is vertical coordinate to concentration expressed in percentage by volume with Radix Salviae Miltiorrhizae fermentation oleo stock prepare liquid, makes Radix Salviae Miltiorrhizae and sends out Ferment oleo stock is shown in Fig. 1 to DPPH free radical scavenging effect curves.
As shown in Figure 1, the gained Radix Salviae Miltiorrhizae of embodiment 1 fermentation oleo stock reaches after diluting 10 times to DPPH free radical scavenging activities 92.57%, the Radix Salviae Miltiorrhizae fermentation oleo stock of 0.934% (concentration expressed in percentage by volume) can remove 50% DPPH free radicals, illustrate that Radix Salviae Miltiorrhizae is sent out There is ferment oleo stock very strong oxidation resistance can remove free radical, promote cellular metabolism, strengthen cell viability, improve the knot of body Structure and function, improve body vitality, so as to delay cell senescence, play the effect of its defying age.
Hydroxyl radical-scavenging is tested:
(1) 2mmol/L FeSO are added in 25ml color comparison tubes4 3ml、1mmol/L H2O23ml, shakes up;Wherein H2O2It is It is eventually adding and starts whole reaction.
(2) 6mmol/L salicylic acid 3ml are subsequently added into, are shaken up;
(3) take out after 37 DEG C of heating in water bath 15min, survey its absorbance A0
(4) certain density prepare liquid 0.2ml, 0.4ml, 0.6ml, 0.8ml, 1.0ml are then respectively adding;
(5) distilled water 0.8ml, 0.6ml, 0.4ml, 0.2ml, 0ml and then are again separately added into, are shaken up, continue heating in water bath 15min, takes out and surveys its absorbance AX
(6) be eliminate after plus common 1.0ml prepare liquids and distilled water caused by system absorbance reduction, method is same On, its absorbance A00, plus 1ml distilled water are surveyed after constant temperature 15min, survey once its absorbance A after shaking up againXX, AReduce=A00- AXX
Radix Salviae Miltiorrhizae ferments, and scavenging action of the oleo stock to hydroxy radical is obvious, and with Radix Salviae Miltiorrhizae fermentation oleo stock concentration increase, it removes hydroxyl The ability of free radical gradually strengthens, and shows the ability of Radix Salviae Miltiorrhizae fermentation oleo stock scavenging hydroxyl and just has significant dose-effect with its concentration Relation.100% Radix Salviae Miltiorrhizae fermentation oleo stock can remove 90.86% hydroxy radical, remove 50% hydroxy radical and need 23.23% (body Product percentage concentration) Radix Salviae Miltiorrhizae fermentation oleo stock.

Claims (10)

1. a kind of preparation method of Radix Salviae Miltiorrhizae fermentation oleo stock, comprises the steps:Radix Salviae Miltiorrhizae dry powder, water and bacterium solution are mixed to get initially System, to the initial system fermentation culture is carried out, and obtains Radix Salviae Miltiorrhizae fermentation oleo stock.
2. preparation method according to claim 1, it is characterised in that:In the preparation method, the bacterial concentration is (105 ~108)CFU/mL;
In the initial system, the proportioning of the bacterium solution, Radix Salviae Miltiorrhizae dry powder and water is (5~10mL):(18~22) g:280mL.
3. preparation method according to claim 1 and 2, it is characterised in that:The temperature of the fermentation culture is 35~45 DEG C; Time is 45~55h.
4. the preparation method according to any one of claim 1-3, it is characterised in that:In the bacterium solution, strain is lactic acid Lactococcus;
The Radix Salviae Miltiorrhizae dry powder is obtained from the under ground portion of Radix Salviae Miltiorrhizae by pulverizer grinding and sieving.
5. according to the preparation method described in claim 4, it is characterised in that:The lactococcus lactis are lactococcus lactis Lactococus lacti。
6. the preparation method according to any one of claim 1-5, it is characterised in that:The preparation method is also included to institute State the fermentation liquid obtained after fermentation culture sterilized successively, be centrifuged, take supernatant obtain Radix Salviae Miltiorrhizae fermentation oleo stock the step of.
7. preparation method according to claim 6, it is characterised in that:The condition of the sterilizing is as follows:Sterilising temp is 115 ~121 DEG C;Sterilization time is 25~35min;
The condition of the centrifugation is as follows:Centrifugal rotational speed is 3800~4200r/min;Centrifugation time is 10~15min;Centrifugation radius For 9cm.
8. the Radix Salviae Miltiorrhizae fermentation oleo stock that the preparation method by any one of claim 1-7 is prepared.
9. described in claim 8 Radix Salviae Miltiorrhizae fermentation oleo stock prepare with following at least one functions cosmetics in application or Application in directly as the cosmetics with following at least one functions:
1) DPPH free radicals are removed;
2) scavenging hydroxyl;
3) antioxidation;
4) defying age.
10. application according to claim 9, it is characterised in that:The cosmetics are facial film, essence or cosmetic water.
CN201611196952.0A 2016-12-22 2016-12-22 Radix salviae miltiorrhizae fermented primary pulp cosmetic product as well as preparation method and applications thereof Pending CN106580789A (en)

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Cited By (5)

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Publication number Priority date Publication date Assignee Title
CN107890441A (en) * 2017-11-24 2018-04-10 无限极(中国)有限公司 A kind of extract and preparation method and application
CN109350648A (en) * 2018-10-10 2019-02-19 广州睿森生物科技有限公司 A kind of Fructus Forsythiae fermenation raw liquid and preparation method thereof
CN112121121A (en) * 2020-09-20 2020-12-25 湖州盛明生物科技有限公司 Salvia miltiorrhiza fermentation concentrated solution
CN113520942A (en) * 2021-07-19 2021-10-22 广州环亚化妆品科技有限公司 Anti-oxidation and anti-aging five-ginseng fermentation product and preparation method and application thereof
WO2023070305A1 (en) * 2021-10-26 2023-05-04 湖州盛明生物科技有限公司 Radix salviae miltiorrhizae fermented beverage and preparation method therefor

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CN105147565A (en) * 2015-09-30 2015-12-16 上海全丽生物科技有限公司 Tomato fermentation puree cosmetic and preparing method and application thereof
CN105997836A (en) * 2016-05-13 2016-10-12 江燕馨 Ferment liquid shampoo and preparation method thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105147565A (en) * 2015-09-30 2015-12-16 上海全丽生物科技有限公司 Tomato fermentation puree cosmetic and preparing method and application thereof
CN105997836A (en) * 2016-05-13 2016-10-12 江燕馨 Ferment liquid shampoo and preparation method thereof

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107890441A (en) * 2017-11-24 2018-04-10 无限极(中国)有限公司 A kind of extract and preparation method and application
CN109350648A (en) * 2018-10-10 2019-02-19 广州睿森生物科技有限公司 A kind of Fructus Forsythiae fermenation raw liquid and preparation method thereof
CN109350648B (en) * 2018-10-10 2021-07-30 广州睿森生物科技有限公司 Forsythia suspensa fermentation stock solution and preparation method thereof
CN112121121A (en) * 2020-09-20 2020-12-25 湖州盛明生物科技有限公司 Salvia miltiorrhiza fermentation concentrated solution
CN113520942A (en) * 2021-07-19 2021-10-22 广州环亚化妆品科技有限公司 Anti-oxidation and anti-aging five-ginseng fermentation product and preparation method and application thereof
WO2023070305A1 (en) * 2021-10-26 2023-05-04 湖州盛明生物科技有限公司 Radix salviae miltiorrhizae fermented beverage and preparation method therefor

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Application publication date: 20170426