CN105147565A - Tomato fermentation puree cosmetic and preparing method and application thereof - Google Patents
Tomato fermentation puree cosmetic and preparing method and application thereof Download PDFInfo
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- CN105147565A CN105147565A CN201510639648.8A CN201510639648A CN105147565A CN 105147565 A CN105147565 A CN 105147565A CN 201510639648 A CN201510639648 A CN 201510639648A CN 105147565 A CN105147565 A CN 105147565A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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Abstract
The invention discloses tomato fermentation puree and a preparing method and application thereof. The method comprises the following step that an initial system composed of lactococcus lactis, tomato puree and water is fermented and cultivated to obtain the tomato fermentation puree. Experimental results show that the prepared tomato fermentation puree does not contain any chemical components, can directly serve as a finished product of a mask or essence or toner, is more natural than other products in the existing market, and has no negative effect on skin; micromolecule active peptide components are obtained, and therefore the puree is prone to being fully absorbed by the skin.
Description
Technical field
The present invention the invention belongs to biological technical field, is specifically related to a kind of fermented tomato oleo stock cosmetics and preparation method thereof and application.
Background technology
Fructus Lycopersici esculenti (LycopersiconesculentumMill.) is Solanaceae Fructus Lycopersici esculenti platymiscium, it is a kind of important solanaceous vegetables, its fruit Fructus Lycopersici esculenti (Fructus Lycopersici esculenti) is not only nutritious, and has antioxidation, defying age, anticancer and protective effect on cancer risk.Containing abundant lycopene, vitamin C and flavone in tamato fruit.Tomato in China aboundresources, for Application and Development Fructus Lycopersici esculenti provides advantage.Develop rapidly along with tomato cultivation industry and range of application is widened, to tomato nutrient composition and in medical care effect, research is also progressively deeply.Also constantly perfect to the extraction process of tomato activated material, but extracting method is mainly based on homogenate extraction method, water extraction.
Summary of the invention
The object of this invention is to provide a kind of Fructus Lycopersici esculenti oleo stock cosmetics and preparation method thereof and application.
The method preparing fermented tomato oleo stock cosmetics provided by the invention, comprises the steps:
Fermentation culture is carried out to the initial system be made up of zymocyte, Fructus Lycopersici esculenti oleo stock and water, obtains described fermented tomato oleo stock.
In said method, the amount ratio of described zymocyte bacterium liquid and described Fructus Lycopersici esculenti oleo stock and water is 24-40ml:10g-20g:300mL, is specially 30mL:16g:300mL; Zymocyte bacterial concentration is 10
4-10
8cFU/mL.
It is Fructus Lycopersici esculenti oleo stock that described Fructus Lycopersici esculenti oleo stock is pulverized through refiner by Fructus Lycopersici esculenti.
In described fermentation culture step, the time is 20h-32h, is specially 28h; Temperature is 30 DEG C-40 DEG C, is specially 35 DEG C.
Described zymocyte is Lactococcus, and specifically can be lactococcus lactis Lactococuslactis, ATCC is numbered 19435.
Described zymocyte exists with the form of its culture fluid or suspension;
The pH value of described zymogenic culture fluid or suspension is 6.5-7.5.
Described method also comprise the steps: fermentation culture gained fermentation liquid to carry out successively sterilizing, centrifugal, get supernatant.
Wherein, in described sterilization steps, sterilising temp is 110-121 DEG C, is specially 115 DEG C; Sterilization time is 15min-30min, is specially 20min;
In described centrifugation step, centrifugal rotational speed is 6400-8000r/min, is specially 6800r/min; Centrifugation time is 30min-40min, is specially 30min; Centrifugal radius is 8cm-12cm, is specially 9cm.
In addition, preparing the application or direct as the application had in the cosmetics of following at least one function had in the cosmetics of following at least one function according to the method described above, also belonging to protection scope of the present invention:
1) whitening;
2) defying age;
3) DPPH free radical is removed;
4) activity of restraint of tyrosinase.
Described cosmetics specifically can be facial film, essence or cosmetic water.
The invention provides a kind of extracting method of fermented tomato thing, the method selects more suitable strain, make product more natural, and do not need to add the foreign substances such as enzyme, not only save production cost, and maximizedly simplify production stage, enable this fermentation technique realize a large amount of production, suitability for industrialized production, and fully can ensure the stability of product quality.Prove by experiment: fermented tomato oleo stock prepared by the present invention, not containing any chemical composition, can directly use as the finished product of facial film or essence or cosmetic water, more natural than existing other products on the market, can not cause any negative interaction to skin; And containing micromolecule polypeptide in fermented ingredient, be easy to allow skin fully absorb.
Accompanying drawing explanation
Fig. 1 is the active polypeptide molecular weight distribution HPLC collection of illustrative plates of fermented tomato oleo stock.
Fig. 2 is the removing DPPH free radical result schematic diagram of fermented tomato oleo stock.
Fig. 3 is the active schematic diagram of restraint of tyrosinase of fermented tomato oleo stock.
Detailed description of the invention
The experimental technique used in following embodiment if no special instructions, is conventional method.
Material used in following embodiment, reagent etc., if no special instructions, all can obtain from commercial channels.
Fluid medium in following embodiment is by solute and solvent composition, and solvent is water, and the concentration in solute and liquid medium within thereof is: glucose 20g/L, peptone 20g/L, yeast extract 10g/L, pH value is 7.0.
YPD culture medium in following embodiment is by solute and solvent composition, solvent is water, concentration in solute and liquid medium within thereof is: culture propagation oleo stock 1% (mass fraction), tryptone 2% (mass fraction), glucose 2% (mass fraction), pH value is 7.0.
Fructus Lycopersici esculenti in following embodiment derives from commercially available.
The ATCC of the lactococcus lactis Lactococuslactis in following embodiment is numbered 19435, obtains from ATCC.
The preparation method of embodiment 1, a kind of fermented tomato oleo stock
1, the activation of strain
Picking lactococcus lactis (Lactococuslactis) bacterium colony one ring, in fluid medium, puts into shaking table by actication of culture, obtains activating rear saccharomyces pastorianus bacterium liquid.
2, the purification of strain
Bacterium liquid gradient dilution bed board after activation step 1 obtained, to obtain single bacterium colony, obtains saccharomyces pastorianus after purification.
3, the amplification culture of strain
Strain inoculation after purification step 2 obtained, in YPD culture medium (pH value is 7.0), is cultivated in the shaking table of 28 DEG C, and as OD value=0.4-1.0, (strain is in logarithmic (log) phase, and concentration is 10 to obtain zymocyte bacterium liquid
4-10
8cFU/ml, pH value is 6.5-7.5).
4, the acquisition of fermented tomato oleo stock
Zymocyte bacterium liquid 30mL step 3 obtained is inoculated in the Fructus Lycopersici esculenti oleo stock of 16g and the water of 300mL, obtains fermentation system (in fermentation system, the proportioning of bacterium liquid total amount and Fructus Lycopersici esculenti and water is 30mL:16g:400g); Fermentation system is fermented 28 hours in 35 DEG C of shaking tables, obtains tunning; By tunning in 115 DEG C of autoclaving 20min, make bacterium inactivation, obtain the tunning after sterilizing; By the centrifugal 30min under 6800r/min, centrifugal radius are the condition of 9cm of the tunning after sterilizing, abandon precipitation, collect supernatant, be fermented tomato oleo stock.
The outward appearance that this embodiment prepares the fermented tomato oleo stock cosmetics of gained is thick liquid, color is faint yellow to brown color.PH value 5.2-6.3, viscosity 200-500cP, solubility is admittedly containing thing content 1.5-5.0%, and total plate count is less than 50CFU/ml, detects without pathogenic bacterium.According to hygienic standards for cosmetics GB7916-87, cosmetics total number of bacteria is not higher than 1000CFU/ml, so this fermentation oleo stock meets cosmetics quality requirement.
Carry out component analysis to these fermented tomato oleo stock cosmetics, protein detection method is with reference to GB5009.5-2010; Crude polysaccharides detection method is with reference to GB/T5009.8-2008; Aminoacid detection method is with reference to GB/T5009.124-2003, and total phenols detection method is with reference to GB/T8313-2008, and acquired results is as follows:
The fermented tomato oleo stock cosmetics that the present invention obtains are containing protein 4.29g/kg, crude polysaccharides 27.82g/kg, total flavones 0.023g/kg (in rutin), total phenols 0.073g/kg.
Following embodiment studies fermented tomato oleo stock direct further as the application in facial film or essence or cosmetic water.
Embodiment 2, fermented tomato oleo stock are as the application in cosmetics
One, the safety of fermented tomato oleo stock detects
Human body patch test is mainly used for the zest detecting cosmetics finished product or raw material.The present invention carries out human closed formula patch test to the fermented tomato oleo stock that embodiment 1 obtains, and is intended to assess its potential skin irritation.
1, subjects
Select suitable volunteer 30 people, the range of age is at 18-60 year Stochastic choice.
2, test method
Taking 0.020g-0.025g solid sample or semisolid sample, to put into speckle examination device for subsequent use.Fluid sample 0.2mL to 0.025mL is dripped on filter paper, then filter paper is placed in speckle examination device.Each sample all arranges blank, in contrast speckle examination device hole, add the sample solvent with sample equivalent, as distilled water or olive oil.
Human body back is elected at test position as, utilizes non-irritating adhesive tape speckle to be tried device and fixedly sticks in experimenter back.Test period continues 24h.In order to the accurate, credible of result of the test and science, test period volunteer as requested, speckle examination device can not be removed, recipient site also can not be made to contact water.Remove speckle examination device after 24h, after leaving standstill 30min, wait for that impression disappears, observe the reaction of skin.If result of the test is negative, then 24h and 48h after patch test is needed to observe once respectively again.
3, result of the test
Patch test results is as shown in table 1-table 3: * "-"=negative reaction; " ± "=suspicious reaction: only have faint erythema; "+"=weak positive reaction (erythematous response): erythema, infiltration, edema, can pimple be had; " ++ "=strong positive reaction (herpes reaction); Erythema, infiltration, edema, pimple, herpes; Reaction can exceed tested district; " +++ "=extremely strong positive reaction (reaction of amalgamation herpes); Obvious erythema, severe infiltration, edema, amalgamation herpes; Reaction exceeds tested district.
According to cosmetics health specification 2007, human body patch test criterion is: occur in 30 routine experimenters that the number of 1 grade of skin adverse reaction is more than 5 examples, or the number of secondary skin adverse reaction is more than 2 examples, or when there is any 1 example more than three grades or three grades skin adverse reactions, then judge that tested material has untoward reaction to human body, otherwise, be then considered as having no adverse reaction to human body.
As can be seen from the table: the fermented tomato oleo stock that embodiment 1 obtains does not produce suspicious reaction, illustrate that fermented tomato thing provided by the invention all has safety, untoward reaction can not be brought to human body.
The patch test results of the fermented tomato oleo stock that table 1, embodiment 1 obtain
Two, the molecular size range of fermented tomato oleo stock detects
Molecular size range checks the key factor whether can carrying out Cutaneous permeation.The present invention measures the molecular size range of fermented tomato oleo stock prepared by embodiment 1 by high performance liquid chromatography (HPLC).Concrete steps are as follows:
1, the reagent of test and the preparation of solution
(1) mobile phase: acetonitrile: water: trifluoroacetic acid=30:70:0.1 (v/v/v).
(2) sample preparation: take mobile phase as solvent, with embodiment 1 gained fermented tomato oleo stock for solute, compound concentration is the sample of 5mg/ml, then for sample introduction after filtering with microporous membrane (0.45 μm).
(3) preparation of standard sample: bovine serum albumin (Mr67000), B12 (Mr1335), oxidized form of glutathione (Mr614) are made into mixed mark, and the concentration of often kind of material is 5mg/ml.
2, test method
Standard substance by three kinds of known molecular amounts: bovine serum albumin (Mw67000), vitamin B
12(Mw1335) and oxidized form of glutathione (Mw614) by high performance liquid chromatograph (Waters company) reference literature " Wang Chengzhong etc. the research of polypeptide molecular weight distribution in protease hydrolyzed wheat germ protein and enzymolysis solution. grain processing; 2012:37 (2) " in method carry out HPLC analysis, draw the standard curve of molecular weight logarithm and elution time.According to gel column permeation chromatography principle, the material that polypeptide molecular weight is large is first by eluting out, and the logarithm of elution time and molecular weight is linear.After the same method fermented tomato oleo stock prepared by the experimental group 1 in embodiment 1, experimental group 2 and experimental group 3 is carried out HPLC analysis again, obtain the elution time of respective peaks, elution time is substituted into standard curve, the molecular size range of fermented tomato oleo stock prepared by embodiment 1 can be obtained.
Chromatographic condition: chromatographic column: TsKgel2000SWXL300mm × 7.8mm; Determined wavelength: UV280nm; Flow velocity: 1ml/min; Column temperature: 30 DEG C.
3, result of the test
The molecular weight of standard substance and the as shown in table 4 of elution time, be figure according to the logarithm of molecular weight and elution time and obtain standard curve: y=-2.4738x+17.656, R
2=0.9998.Wherein y is elution time (min); X is molecular weight logarithm.
The molecular weight of table 4, standard substance and elution time
Experimental group 1 prepare fermented tomato oleo stock HPLC collection of illustrative plates as shown in Figure 1: as can be seen from the figure, 1 component peaks is mainly comprised in the collection of illustrative plates of fermented tomato oleo stock, elution time is 11.72min, elution time is substituted into standard curve: y=-2.4738x+17.656, the molecular weight calculating peak is 250.93Da, and accounts for 89.33% according to calculated by peak area fermented tomato oleo stock.It is generally acknowledged that molecular weight about 500Da bioactive peptide composition is more easily absorbed by the skin, so there is absorbable active polypeptide in fermented tomato thing.
Three, the antioxygenic property of fermented tomato oleo stock detects
DPPH is a kind of organic free radical of early stage synthesis, be commonly used to the hydrogen supply capacity assessing polyphenoils, it is highly stable in organic solvent, in purple, and have a characteristic absorption peak at 517nm place, when running into free radical scavenger, the lone pair electrons of DPPH are paired and make it fade, and namely diminish at the light absorption value of maximum absorption wave strong point.Therefore, the change by measuring light absorption value carrys out the elimination effect of assess sample to DPPH free radical.
The specific experiment step of DPPH free radical scavenging experiment is:
Using embodiment 1 gained fermented tomato oleo stock as determinand;
(1) liquid to be measured and 2 × 10 of equal-volume (being generally 3mL) is got
-4the DPPH solution mixing (A of mol/L
1pipe);
(2) isopyknic dehydrated alcohol (determinand solvent) and 2 × 10 is got
-4the DPPH solution mixing (A of mol/L
2pipe);
(3) isopyknic dehydrated alcohol is got and determinand mixes (A
3pipe);
(4), after reacting 30min, under 517nm, A is surveyed
1, A
2, A
3pipe absorbance.
Clearance rate computing formula is: clearance rate (%)=[(A
2+ A
3)-A
1]/A
2
Make fermented tomato oleo stock to DPPH free radical scavenging effect curves, see accompanying drawing 2.
As seen from the figure, embodiment 1 gained fermented tomato oleo stock is to the IC of DPPH scavenging action
50=7.50%, illustrate that fermented tomato oleo stock has certain oxidation resistance, can scavenging free radicals, promote cellular metabolism, strengthen cell viability, improve the 26S Proteasome Structure and Function of body, improve body vitality, thus delay cell senescence, play its antidotal effect.
Four, the white-skinned face function analysis of fermented tomato oleo stock
Tryrosinase is melanogenic key enzyme, which control the forming process of melanocyte, and the deposition of its level of activity to pigment plays a major role.Many whitenings of selling in the market, speckle dispelling product are all reach whitening function with restraint of tyrosinase, therefore are evaluate the leading indicator of whitening cosmetic to the power of tyrosinase inhibitory action.
Carried out the whitening function of assess sample by the impact of working sample on tryrosinase, concrete grammar is as follows:
Solution is configured by table 5:
Table 5, solution preparation list
| Unit (mL) | C 1 | C 2 | T 1 | T 2 |
| TYR | 2 | 2 | 2 | 2 |
| Sample | 0 | 0 | 2 | 2 |
| PBS | 4 | 5 | 2 | 3 |
| Tryrosinase | 1 | 0 | 1 | 0 |
| Cumulative volume | 7 | 7 | 7 | 7 |
Note: C
1and T
1add 1mL tryrosinase, enzyme is lived as 100U/mL.
Sample is embodiment 1 gained fermented tomato oleo stock;
(1) C
2after pipe prepares and shakes up, heating in water bath 10min in 37 DEG C of water-baths, returns to zero under wavelength 475nm.
(2) C
1pipe solution prepares and shakes up, and after 37 DEG C of water-bath 10min, adds tryrosinase 1ml, continues water-bath 10 minutes, measures C
1absorbance.
(3) by (1) (2) same method, with T
2zeroing measures T
1absorbance.
(4) calculation sample is to the maximum inhibition T (%) of tryrosinase.T(％)＝(C
1-T
1)/C
1×100％
Experiment prove, the embodiment of the present invention 1 prepare gained fermented tomato oleo stock to the suppression ratio of tyrosinase activity be the arbutin of 56.04%, 1% to the suppression ratio 99.52% of tyrosinase activity, see accompanying drawing 3.Namely the whitening effect of fermented tomato oleo stock provided by the invention is equivalent to the effect of the arbutin of 0.563%, has certain white-skinned face function.
Claims (10)
1. prepare a method for fermented tomato oleo stock, comprising the steps: that the initial system to being made up of zymocyte, Fructus Lycopersici esculenti oleo stock and water carries out fermentation culture, obtaining described fermented tomato oleo stock.
2. method according to claim 1, is characterized in that: the amount ratio of described zymocyte bacterium liquid and described Fructus Lycopersici esculenti oleo stock and water is 24-40ml:10g-20g:300mL, and zymocyte bacterial concentration is 10
4-10
8cFU/mL.
3. method according to claim 1 and 2, is characterized in that: in described fermentation culture step, and the time is 20h-32h; Temperature is 30 DEG C-40 DEG C.
4., according to described method arbitrary in claim 1-3, it is characterized in that: described zymocyte is Lactococcus, be specially lactococcus lactis Lactococuslactis.
5. according to described method arbitrary in claim 1-4, it is characterized in that: described zymocyte exists with the form of its culture fluid or suspension;
The pH value of described zymogenic culture fluid or suspension is 6.5-7.5.
6., according to described method arbitrary in claim 1-5, it is characterized in that: described method also comprise the steps: fermentation culture gained fermentation liquid to carry out successively sterilizing, centrifugal, get supernatant.
7. method according to claim 6, is characterized in that: in described sterilization steps, and sterilising temp is 110-121 DEG C; Sterilization time is 15min-30min;
In described centrifugation step, centrifugal rotational speed is 6400-8000r/min; Centrifugation time is 30min-40min; Centrifugal radius is 8cm-12cm.
8. the fermented tomato oleo stock that in claim 1-7, arbitrary described method prepares.
9. fermented tomato oleo stock described in claim 8 is preparing the application or direct as the application had in the cosmetics of following at least one function had in the cosmetics of following at least one function according to the method described above:
1) whitening;
2) defying age;
3) DPPH free radical is removed;
4) activity of restraint of tyrosinase.
10. application according to claim 9, is characterized in that: described cosmetics are facial film, essence or cosmetic water.
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| CN112089664A (en) * | 2020-10-21 | 2020-12-18 | 广州华晟科创科技发展有限公司 | Essence with effects of resisting aging and removing deep wrinkles and preparation method thereof |
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| CN112089664A (en) * | 2020-10-21 | 2020-12-18 | 广州华晟科创科技发展有限公司 | Essence with effects of resisting aging and removing deep wrinkles and preparation method thereof |
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Effective date of registration: 20201109 Address after: No.6, 18-19, Lane 666, Xianing Road, Jinshan District, Shanghai, 201506 Patentee after: SHANGHAI BIOTRULY BIOTECHNOLOGY Co.,Ltd. Address before: 100048, Fu Cheng Road, Beijing, Haidian District, No. 33 Patentee before: BEIJING TECHNOLOGY AND BUSINESS University Patentee before: SHANGHAI BIOTRULY BIOTECHNOLOGY Co.,Ltd. |