CN105147565B - A kind of fermented tomato magma cosmetics and the preparation method and application thereof - Google Patents
A kind of fermented tomato magma cosmetics and the preparation method and application thereof Download PDFInfo
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- CN105147565B CN105147565B CN201510639648.8A CN201510639648A CN105147565B CN 105147565 B CN105147565 B CN 105147565B CN 201510639648 A CN201510639648 A CN 201510639648A CN 105147565 B CN105147565 B CN 105147565B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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Abstract
The invention discloses a kind of fermented tomato magma and the preparation method and application thereof.This method comprises the following steps：Fermented and cultured is carried out to the initial system being made of Lactococcus lactis, tomato magma and water, obtains the fermented tomato magma.It is experimentally confirmed：Fermented tomato magma prepared by the present invention is free of any chemical composition, can be used directly as the finished product of facial mask or Essence or toner, more natural than existing other products on the market, and any negative interaction will not be caused to skin；And small molecule active peptide components are obtained, it is easy to that skin is allowed to fully absorb.
Description
Technical field
Invention belongs to biotechnology, and in particular to a kind of fermented tomato magma cosmetics and its preparation side
Method and application.
Background technology
Tomato (Lycopersiconesculentum Mill.) is Solanaceae tomato platymiscium, is a kind of important solanaceous vegetables
Vegetables, fruit tomato (tomato) is not only full of nutrition, and has anti-oxidant, anti-aging, anticancer and protective effect on cancer risk.Tomato
Contain abundant lycopene, vitamin C and flavones in fruit.Tomato in China is resourceful, is provided with for development and application tomato
Sharp condition.As the rapid development of tomato cultivation industry and application range are widened, to tomato nutritional ingredient and in medical care effect side
Face research is also gradually goed deep into.To the extraction process of tomato activated substance also constantly improve, but extracting method is mainly carried with flash
It follows the example of, based on water extraction.
Invention content
The object of the present invention is to provide a kind of tomato magma cosmetics and the preparation method and application thereof.
The method provided by the invention for preparing fermented tomato magma cosmetics, includes the following steps：
Fermented and cultured is carried out to the initial system being made of zymophyte, tomato magma and water, it is former to obtain the fermented tomato
Slurry.
In the above method, the zymophyte bacterium solution and the amount ratio of the tomato magma and water are 24-40ml：10g-20g：
300mL, specially 30mL：16g：300mL；Zymophyte bacterial concentration is 104-108CFU/mL。
It is tomato magma that the tomato magma, which is crushed by tomato through refiner,.
In the fermented and cultured step, time 20h-32h, specially 28h；Temperature be 30 DEG C -40 DEG C, specially 35
℃。
The zymophyte is galactococcus, concretely Lactococcus lactis Lactococuslactis, and ATCC numbers are
19435。
The zymophyte exists in the form of its culture solution or suspension；
The pH value of the zymogenic culture solution or suspension is 6.5-7.5.
The method further includes following steps：Zymotic fluid obtained by fermented and cultured is sterilized successively, centrifuged, takes supernatant
Liquid.
Wherein, in the sterilization steps, sterilising temp is 110-121 DEG C, specially 115 DEG C；Sterilization time is 15min-
30min, specially 20min；
In the centrifugation step, centrifugal rotational speed 6400-8000r/min, specially 6800r/min；Centrifugation time is
30min-40min, specially 30min；Centrifugation radius is 8cm-12cm, specially 9cm.
In addition, according to the method described above the application in preparing the cosmetics with following at least one function or directly as
Application in cosmetics with following at least one function, also belongs to protection scope of the present invention：
1) whitening；
2) anti-aging；
3) DPPH free radicals are removed；
4) inhibit the activity of tyrosinase.
The cosmetics concretely facial mask, Essence or toner.
The present invention provides a kind of extracting method of fermented tomato object, this method selects more suitable strain, makes product
It is more natural, and the foreign substances such as enzyme need not be added, production cost is not only saved, but also maximumlly simplify production step
Suddenly, so that the fermentation technique is realized mass production, industrialized production, and can fully ensure that the stability of product quality.It is logical
Cross experiments have shown that：Fermented tomato magma prepared by the present invention is free of any chemical composition, can be directly as facial mask or Essence
Or the finished product of toner uses, and it is more natural than existing other products on the market, any negative interaction will not be caused to skin；And
Contain micromolecule polypeptide in fermented ingredient, is easy to that skin is allowed to fully absorb.
Description of the drawings
Fig. 1 is the active peptides molecular weight distribution HPLC collection of illustrative plates of fermented tomato magma.
Fig. 2 is the removing DPPH free radical result schematic diagrams of fermented tomato magma.
Fig. 3 inhibits tyrosinase activity schematic diagram for fermented tomato magma.
Specific implementation mode
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Fluid nutrient medium in following embodiments is made of solute and solvent, and solvent is water, solute and its in Liquid Culture
It is a concentration of in base：Glucose 20g/L, peptone 20g/L, yeast extract 10g/L, pH value 7.0.
YPD culture mediums in following embodiments are made of solute and solvent, and solvent is water, solute and its liquid medium within
In it is a concentration of：Yeast fermentation magma 1% (mass fraction), tryptone 2% (mass fraction), (quality point of glucose 2%
Number), pH value 7.0.
Tomato in following embodiments is from commercially available.
The ATCC numbers of Lactococcus lactis Lactococuslactis in following embodiments are 19435, are obtained from ATCC.
Embodiment 1, a kind of preparation method of fermented tomato magma
1, the activation of strain
One ring of picking Lactococcus lactis (Lactococuslactis) bacterium colony is put into shaking table in fluid nutrient medium by bacterium
Kind activation, saccharomyces pastorianus bacterium solution after being activated.
2, the purifying of strain
Bacterium solution gradient dilution bed board after the activation that step 1 is obtained obtains Pasteur's ferment after purification to obtain single bacterium colony
It is female.
3, the expansion culture of strain
The strain after purification that step 2 obtains is inoculated into YPD culture mediums (pH value 7.0), in 28 DEG C of shaking table
Culture, as OD values=0.4-1.0, obtain zymophyte bacterium solution (strain is in logarithmic phase, a concentration of 104-108CFU/ml, pH value
For 6.5-7.5).
4, the acquisition of fermented tomato magma
The zymophyte bacterium solution that 30mL steps 3 obtain is inoculated into the tomato magma of 16g and the water of 300mL, is fermented
(proportioning of bacterium solution total amount and tomato and water is 30mL to system in fermentation system：16g：400g)；By fermentation system in 35 DEG C of shaking tables
Middle fermentation 28 hours, obtains tunning；By tunning in 115 DEG C of high pressure sterilization 20min, bacterium is set to inactivate, after obtaining sterilizing
Tunning；Tunning after sterilizing is centrifuged into 30min under conditions of 6800r/min, centrifugation radius are 9cm, it is heavy to abandon
It forms sediment, collects supernatant, as fermented tomato magma.
The appearance that the embodiment prepares the fermented tomato magma cosmetics of gained is thick liquid, color is faint yellow to brown
Yellow.PH value 5.2-6.3, viscosity 200-500cP, soluble solids content 1.5-5.0%, total plate count is less than 50CFU/
Ml, no pathogenic bacteria detection.According to hygienic standards for cosmetics GB7916-87, cosmetics total number of bacteria is not higher than 1000CFU/ml, institute
Meet cosmetics quality requirement with this magma of fermenting.
Constituent analysis is carried out to the fermented tomato magma cosmetics, protein detection method is with reference to GB5009.5-2010；Slightly
Polysaccharide detection method is with reference to GB/T 5009.8-2008；Amino acid detection method is with reference to GB/T 5009.124-2003, total phenol inspection
For survey method with reference to GB/T8313-2008, acquired results are as follows：
Fermented tomato magma cosmetics 4.29g/kg containing protein produced by the present invention, Thick many candies 27.82g/kg, general flavone
0.023g/kg (in terms of rutin), total phenol 0.073g/kg.
Following embodiments further study fermented tomato magma directly as answering in facial mask or Essence or toner
With.
Embodiment 2, fermented tomato magma are as the application in cosmetics
One, the safety detection of fermented tomato magma
Human body patch test is mainly used for detecting the irritation of cosmetics finished product or raw material.The present invention is to embodiment 1
The fermented tomato magma of acquisition carries out human closed formula patch test, it is intended to assess its potential skin irritation.
1, subjects
30 people of suitable volunteer, the range of age is selected to be randomly choosed at 18-60 Sui.
2, test method
Weigh 0.020g-0.025g solid samples or semisolid sample be put into it is spare in spot examination device.By fluid sample
0.2mL to 0.025mL is added dropwise on filter paper, then filter paper is placed in spot examination device.Each sample is respectively provided with blank control,
It compares in spot examination device hole and the sample solvent with sample equivalent is added, such as distilled water or olive oil.
Experiment position is selected as human body back, and spot examination device fixation is pasted on subject back using nonirritant adhesive tape.
Test period continues for 24 hours.For accurate, the credible and science of test result, dduring test volunteer as requested, Bu Nengzhai
Fall spot examination device, recipient site can not also be made to contact water.Removal spot tries device afterwards for 24 hours, after standing 30min, impression is waited for disappear, observation
The reaction of skin.If test result is feminine gender, need to observe again respectively with 48h for 24 hours after patch test primary.
3, test result
Patch test results are as shown in table 1- tables 3：* "-"=negative reaction；" ± "=suspicious reaction：It is only faint red
Spot；"+"=weakly positive reaction (erythematous response)：Erythema, oedema, can have papule at infiltration；(bleb is anti-for " ++ "=strong positive reaction
It answers)；Erythema, infiltration, oedema, papule, bleb；Reaction can exceed tested area；" +++ "=extremely strong positive reaction (amalgamation bleb
Reaction)；Apparent erythema, severe infiltration, oedema, amalgamation bleb；Reaction exceeds tested area.
According to cosmetics health specification 2007, human body patch test criterion is：Occurs 1 grade of skin in 30 subjects
The number of adverse reaction is more than 5 or the number of two level skin adverse reaction is more than 2, or any 1 three-level or three-level occurs
When the above skin adverse reaction, then judge that tested material has adverse reaction to human body, has no adverse reaction to human body conversely, being then considered as.
As can be seen from the table：The fermented tomato magma that embodiment 1 obtains does not generate suspicious reaction, illustrates offer of the present invention
Fermented tomato object all have safety, adverse reaction will not be brought to human body.
The patch test results for the fermented tomato magma that table 1, embodiment 1 obtain
Two, the molecular size range detection of fermented tomato magma
Molecular size range is an important factor for checking whether that Cutaneous permeation can be carried out.The present invention passes through high performance liquid chromatography
(HPLC) molecular size range of fermented tomato magma prepared by embodiment 1 is measured.It is as follows：
1, the preparation for the reagent and solution tested
(1) mobile phase：Acetonitrile：Water：Trifluoroacetic acid=30：70：0.1(v/v/v).
(2) sample preparation：Using mobile phase as solvent, using 1 gained fermented tomato magma of embodiment as solute, compound concentration is
The sample of 5mg/ml, then for sample introduction after with (0.45 μm) filtering of microporous barrier.
(3) prepared by standard sample：By bovine serum albumin (Mr 67000), B12 (Mr 1335), oxidized form of glutathione (Mr
614) it is made into mixed mark, the concentration of each substance is 5mg/ml.
2, test method
By the standard items of three kinds of known molecular amounts：Bovine serum albumin(BSA) (Mw67000), vitamin B12(Mw1335) it and aoxidizes
Type glutathione (Mw614) passes through high performance liquid chromatograph (Waters companies) reference literature " protease hydrolyzed wheats such as Wang Chengzhong
The research grain processings that polypeptide molecular weight is distributed in embryo egg bletilla enzymolysis liquid, 2012:Method in 37 (2) " carries out HPLC points
The standard curve of molecular weight logarithm and elution time is drawn in analysis.According to gel column permeation chromatography principle, the big object of polypeptide molecular weight
Matter first is eluted out, and the logarithm of elution time and molecular weight is in a linear relationship.It will be in embodiment 1 according still further to same method
Fermented tomato magma prepared by experimental group 1, experimental group 2 and experimental group 3 carries out HPLC analyses, obtains the elution time of respective peaks,
Elution time is substituted into standard curve, you can obtain the molecular size range of the fermented tomato magma of the preparation of embodiment 1.
Chromatographic condition：Chromatographic column：TsK gel 2000 SWXL 300mm×7.8mm；Detection wavelength：UV 280nm；Stream
Speed：1ml/min；Column temperature：30℃.
3, test result
The molecular weight of standard substance and elution time it is as shown in table 4, figure is done according to the logarithm of molecular weight and elution time
Obtain standard curve：Y=-2.4738x+17.656, R2=0.9998.Wherein y is elution time (min)；X is molecular weight pair
Number.
Table 4, the molecular weight of standard substance and elution time
The HPLC collection of illustrative plates of fermented tomato magma prepared by experimental group 1 is as shown in Figure 1：It can be seen from the figure that fermented tomato
Include mainly 1 component peaks in the collection of illustrative plates of magma, elution time is substituted into standard curve by elution time 11.72min：Y=-
2.4738x+17.656, the molecular weight for calculating appearance is 250.93Da, and is accounted for according to calculated by peak area fermented tomato magma
89.33%.It is generally acknowledged that molecular weight 500Da or so activity peptide components are more easily absorbed by the skin, so existing in fermented tomato object
Absorbable active peptides.
Three, the antioxygenic property detection of fermented tomato magma
DPPH is a kind of organic free radical of early stage synthesis, is commonly used to the hydrogen supply capacity of assessment polyphenoils, it is organic
It is highly stable in solvent, it is in purple, and there are one characteristic absorption peaks at 517nm, when encountering free radical scavenger, DPPH
Lone pair electrons be paired and it made to fade, that is, become smaller in the light absorption value of maximum absorption wave strong point.Therefore, measurement can be passed through
Light absorption value changes to evaluate elimination effect of the sample to DPPH free radicals.
DPPH radicals scavengings experiment specific experiment step be：
Using 1 gained fermented tomato magma of embodiment as determinand；
(1) prepare liquid and 2 × 10 of isometric (generally 3mL) is taken-4The DPPH solution mixings (A of mol/L1Pipe)；
(2) isometric absolute ethyl alcohol (determinand solvent) and 2 × 10 is taken-4The DPPH solution mixings (A of mol/L2Pipe)；
(3) isometric absolute ethyl alcohol and determinand mixing (A are taken3Pipe)；
(4) after reacting 30min, A is surveyed at 517nm1、A2、A3Pipe absorbance value.
Clearance rate calculation formula is：Clearance rate (%)=[(A2+A3)-A1]/A2
Fermented tomato magma is made to DPPH radicals scavenging effect curves, sees attached drawing 2.
As seen from the figure, IC of the 1 gained fermented tomato magma of embodiment to DPPH scavenging effects50=7.50%, illustrate tomato
Magma of fermenting has certain oxidation resistance, can remove free radical, promotes cell metabolism, enhances cell viability, improves body
Structure and function, improve body vitality play the effect of its anti-aging to delay cell senescence.
Four, the white-skinned face function analysis of fermented tomato magma
Tyrosinase is the key enzyme of melanogenesis, it controls the forming process of melanocyte, and level of activity is to pigment
Deposition plays a major role.Current market sales of many whitenings, nti-freckle product are made with inhibiting tyrosinase to reach whitening
With, therefore be to evaluate the leading indicator of whitening cosmetic to the power of tyrosinase inhibitory action.
The whitening function of sample is evaluated to the influence of tyrosinase by determination sample, the specific method is as follows：
Solution is configured by table 5：
Table 5, solution prepare list
| Unit (mL) | C1 | C2 | T1 | T2 |
| L-tyrosine | 2 | 2 | 2 | 2 |
| Sample | 0 | 0 | 2 | 2 |
| PBS | 4 | 5 | 2 | 3 |
| Tyrosinase | 1 | 0 | 1 | 0 |
| Total volume | 7 | 7 | 7 | 7 |
Note：C1And T11mL tyrosinases, enzyme activity 100U/mL is added.
Sample is 1 gained fermented tomato magma of embodiment；
(1)C2Pipe prepares shake up after, return to zero under the heating water bath 10min in 37 DEG C of water-baths, wavelength 475nm.
(2)C1Pipe solution, which prepares, to be shaken up, and after 37 DEG C of water-bath 10min, is added tyrosinase 1ml, is continued water-bath 10 minutes, measures
C1Absorbance value.
(3) (1) (2) same method is pressed, with T2Zeroing measures T1Absorbance value.
(4) maximum inhibition T (%) of the sample to tyrosinase is calculated.T (%)=(C1-T1)/C1× 100%
It is demonstrated experimentally that the preparation gained fermented tomato magma of the embodiment of the present invention 1 is to the inhibiting rate of tyrosinase activity
56.04%, 1% ursin is shown in attached drawing 3 to the inhibiting rate 99.52% of tyrosinase activity.Tomato hair i.e. provided by the invention
The whitening effect of ferment magma is equivalent to the effect of 0.563% ursin, has certain white-skinned face function.
Claims (6)
1. a kind of method preparing fermented tomato magma, includes the following steps：It is first to being made of zymophyte, tomato magma and water
Initial body system carries out fermented and cultured, obtains the fermented tomato magma；
The zymophyte bacterium solution and the amount ratio of the tomato magma and water are 24-40ml：10g-20g：300mL, zymophyte bacterium
Liquid a concentration of 104-108CFU/mL；
In the fermented and cultured step, time 20h-32h；Temperature is 30 DEG C -40 DEG C；
The zymophyte is Lactococcus lactis Lactococuslactis；
The zymophyte exists in the form of its culture solution or suspension；
The pH value of the zymogenic culture solution or suspension is 6.5-7.5.
2. according to the method described in claim 1, it is characterized in that：The method further includes following steps：By fermented and cultured institute
Zymotic fluid is obtained to be sterilized successively, centrifuge, take supernatant.
3. according to the method described in claim 2, it is characterized in that：In the sterilization steps, sterilising temp is 110-121 DEG C；
Sterilization time is 15min-30min；
In the centrifugation step, centrifugal rotational speed 6400-8000r/min；Centrifugation time is 30min-40min；Centrifuging radius is
8cm-12cm。
4. the fermented tomato magma that any the method is prepared in claim 1-3.
5. fermented tomato magma described in claim 4 is preparing the makeup with following at least one function according to the method described above
Application in product or directly as the application in the cosmetics with following at least one function：
1) whitening；
2) anti-aging；
3) DPPH free radicals are removed；
4) inhibit the activity of tyrosinase.
6. application according to claim 5, it is characterised in that：The cosmetics are facial mask, Essence or toner.
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| CN112089664B (en) * | 2020-10-21 | 2021-09-07 | 广州市万千粉丝化妆品有限公司 | Essence with effects of resisting aging and removing deep wrinkles and preparation method thereof |
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| CN103610632A (en) * | 2013-11-06 | 2014-03-05 | 曹书林 | Beauty cream prepared by fermenting tomato composition |
| CN104164452A (en) * | 2014-08-19 | 2014-11-26 | 云南中烟工业有限责任公司 | Solanum lycopersicum fermentation broth extract as well as preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103610632A (en) * | 2013-11-06 | 2014-03-05 | 曹书林 | Beauty cream prepared by fermenting tomato composition |
| CN104164452A (en) * | 2014-08-19 | 2014-11-26 | 云南中烟工业有限责任公司 | Solanum lycopersicum fermentation broth extract as well as preparation method and application thereof |
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