CN108852973A - A kind of Semen Coicis extractive from fermentative and its preparation method and application - Google Patents
A kind of Semen Coicis extractive from fermentative and its preparation method and application Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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Abstract
The present invention relates to cosmetic technical field, specifically a kind of Semen Coicis extractive from fermentative and preparation method thereof includes the following steps：Ingredient：Zymophyte：Semen Coicis powder：Water=5~10mL：10-15g, 250~350mL；Prepare initial system：The Semen Coicis powder, zymophyte, water are sufficiently mixed, obtain initial system, fermentation bacteria concentration is 10 in the initial system5‑106CFU/ml；Fermentation：Fermentation process is carried out to initial system, obtains fermentation liquid；Obtain finished product：It successively sterilized to fermentation liquid, collect supernatant after centrifugal treating, obtain the Semen Coicis extractive from fermentative；The Semen Coicis powder is the powder of Semen Coicis root；The zymophyte uses the bacterium solution of saccharomycete；Compared to the prior art the present invention, effectively maintains the natural activity of plant, improve safety of the product to human body.
Description
Technical field
The present invention relates to cosmetic technical field, specifically a kind of Semen Coicis extractive from fermentative and preparation method thereof and answer
With.
Background technique
Semen Coicis is adlay category gramineae plant, wide geographic area of the main product on the south 33 ° of N.Seed, root, stem, leaf of adlay etc.
It can be utilized by the mankind, nutritive value and medical value are all that other plant cannot compare, and therefore, are known as
" king of world gramineae plant ".It is both food and the kind list of drug that adlay is classified as by the Ministry of Public Health now.
Currently, the cosmetics in the market containing Semen Coicis ingredient, be all added in cosmetic base formulations it is a certain proportion of
Semen Coicis extract, additive amount is lower, while being limited by extraction conditions, and contained substance ingredient is limited in extract, cannot be abundant
Play the beauty functions of Semen Coicis.
Therefore, there are also to be developed for the prior art.
Summary of the invention
Place in view of above-mentioned deficiencies of the prior art, the purpose of the present invention is to provide a kind of Semen Coicis extractive from fermentative and its
Preparation method.The problem of aiming to solve the problem that existing extractive technique inefficiency.
In order to achieve the above object, the present invention provides a kind of preparation method of Semen Coicis extractive from fermentative, which is characterized in that packet
Include following steps：
1) ingredient：Ingredient：Zymophyte：Semen Coicis powder：Water=5~10mL：10-15g, 250~350mL；The zymophyte
Concentration is 105-106CFU/ml；
2) initial system is prepared：The Semen Coicis powder, zymophyte, water are sufficiently mixed, initial system is obtained；
3) it ferments：Fermentation process is carried out to initial system, obtains fermentation liquid；
4) finished product is obtained：It successively sterilized to fermentation liquid, collect supernatant after centrifugal treating, obtained the Semen Coicis fermentation and mention
Take object；
The zymophyte uses the bacterium solution of saccharomycete.
Further, the saccharomycete uses red phaffia rhodozyma.
Further, the mesh number of the Semen Coicis powder is 20-40 mesh, specially 30 mesh.
Further, the initial system be take zymogenic bacterium solution 8ml, 30 mesh coixlacrymajobi powder 10g and water 300ml mix and
?.
Further, fermentation temperature is 20-30 DEG C in the fermentation, and fermentation time is 35-40 hours.
The fermentation temperature of the fermentation is 28 DEG C.
Further, the centrifugal rotational speed is 10000-12000r/min, and centrifugation radius is 9-12cm；Centrifugation time is
15-30min, specially 22min.
The present invention also provides the Semen Coicis extractive from fermentative of the preparation method preparation described in one kind.
The present invention also provides the Semen Coicis extractive from fermentative in the cosmetics that preparation has following at least one functions
Application or directly as the application in the cosmetics with following at least one functions：
1) DPPH free radical is removed；
2) it inhibits tyrosinase activity；
3) moisturizing.
Further, the cosmetics are facial mask, Essence or toner.
Compared with prior art, the present invention having following advantage：
(1) present invention carries out whole plant fermentation to Semen Coicis using red phaffia rhodozyma, remains whole functional components of plant
And its activity, avoid the loss of active constituent caused by extracting method.
(2) fermentation method of the present invention does not add any organic examination in Semen Coicis effective component extraction process
Agent, fermentation temperature and fermentation pH are mild, and active components of plants structure is not destroyed, and keeps the natural activity of plant.
(3) Semen Coicis extract provided by the invention after fermentation, can be used as cosmetics and directly use, in magma of fermenting not
Essence etc. is added to chemical component, it is ensured that safety of the product to human body.
(4) it is rich in have in Semen Coicis extractive from fermentative provided by the invention and inhibits tyrosinase activity and melanin is inhibited to close
At active material, thus to skin have stronger whitening function, while with the ingredient in extractive from fermentative have cooperate with work
With.
(5) Semen Coicis extractive from fermentative cosmetics provided by the invention have the function of slowing down skin water loss, to reach
To the effect of moisturizing.
(6) Semen Coicis extractive from fermentative provided by the invention has molecular weight small, is easy to the advantages of skin absorbs.
Detailed description of the invention
Fig. 1 is that Semen Coicis extractive from fermentative cosmetics remove DPPH free radical result schematic diagram；
Fig. 2 is that Semen Coicis extractive from fermentative cosmetics inhibit tyrosinase activity result schematic diagram；
Fig. 3 is Semen Coicis extractive from fermentative molecular weight schematic diagram；
Fig. 4 is that Semen Coicis extractive from fermentative cosmetics slow down skin water loss result schematic diagram.
Specific embodiment
Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings, it should be understood that preferred reality described herein
Apply example only for the purpose of illustrating and explaining the present invention and is not intended to limit the present invention.
Unless otherwise specified, the conventional means that technological means used in embodiment is well known to those skilled in the art.
Semen Coicis used in the present invention is commercially available Semen Coicis, eliminates larger-size particle by screening after crushing, retains mesh number
For the Semen Coicis powder of 20-40 mesh.
Red phaffia rhodozyma (Phaffia rhodozyma) used in the present invention is received purchased from Su Zhoubei creates the connection limited public affairs of biotechnology
Department, rhodotorula mucilaginosa, lactobacillus, saccharomyces cerevisiae are purchased from China General Microbiological Culture Collection Center.
Red phaffia rhodozyma used in following embodiments is pre-processed in accordance with the following steps：
Fermentation process first activates strain, makes to keep higher viability；Then bacterium is purified, makes to make when fermentation
Use single culture；Then spreading cultivation makes bacterium mass propagation.Item of the mixed liquor of bacterium access coixlacrymajobi powder and water after spreading cultivation in adaptation
It ferments under part, is then centrifuged for that supernatant is taken to be detected.Detailed process is as follows：
(1) actication of culture：Picking colony is put into fluid nutrient medium from the inclined-plane of preservation, is put into shaking table strain is living
Change.The activation uses the activation method of strain routine, does not make expansion description in the present invention.
(2) purifying agaric：By the strain gradient dilution bed board of activation, to obtain single colonie.
(3) strain spreads cultivation：Stand-by strain is inoculated into respective liquid culture medium, is cultivated in 20-30 DEG C of shaking table, until
When its OD value=0.5-1.0, strain is in logarithmic phase, as suitable inoculum density 105-106CFU/ml.Used medium is
Wort agar medium forms as follows：Yeast extract 100mL, agar 1.5g.
Embodiment 1
It is 1o by the fermentation bacteria concentration to spread cultivation in culture medium5-106The zymophyte bacterium solution 8mL of CFU/ml is inoculated into 30 mesh 10g
Coixlacrymajobi powder and 300mL water in, and after carrying out in 28 DEG C of shaking tables fermentation 40 hours, gained fermentation liquid is carried out 115 DEG C
High pressure sterilization, 20min high pressure sterilization, inactivates bacterium, in 12000r/min, centrifugation radius is 9cm by fermentation liquid after sterilizing
Under the conditions of be centrifuged 20min, collect supernatant, Semen Coicis extractive from fermentative as provided by the invention.
The embodiment prepares resulting Semen Coicis extractive from fermentative and can use directly as cosmetics, and appearance is viscous fluid
Body, color are pale pink to brownish red.PH value 5.2-6.8, viscosity 200-500cP, soluble solids content 2-5.0%, bacterium
It falls sum and is less than 50CFU/ml, no pathogenic bacteria detection meets cosmetics quality requirement.
Constituent analysis is carried out to the Semen Coicis extractive from fermentative cosmetics, protein detection method is referring to GB5009.5-2010；
Thick many candies detection method is referring to GB/T 5009.8-2008；Amino acid detection method knot referring to obtained by GB/T 5009.124-2003
Fruit is as follows：
Constituent analysis (g/kg) of 1 different strains of table to Semen Coicis extractive from fermentative
| Containing Thick many candies | General flavone | Total phenol | Total protein | |
| Rhodotorula mucilaginosa | 5.23 | 0.15 | 0.04 | 1.6 |
| Lactobacillus | 4.02 | 0.13 | 0.03 | 1.3 |
| Saccharomyces cerevisiae | 7.15 | 0.16 | 0.03 | 1.4 |
| Red phaffia rhodozyma | 10.07 | 0.33 | 0.08 | 4.4 |
As described in Table 1, Semen Coicis extractive from fermentative cosmetics 4.4g/kg containing protein, Thick many candies made from red phaffia rhodozyma
10.07g/kg, general flavone 0.033g/kg (in terms of rutin), total phenol 0.08g/kg.Therefore, to Semen Coicis made from red phaffia rhodozyma
Extractive from fermentative is further analyzed.
Embodiment 2
DPPH is a kind of organic free radical of early stage synthesis, is commonly used to the hydrogen supply capacity of assessment polyphenoils, it is organic
It is highly stable in solvent, it is purple, and have a characteristic absorption peak, when encountering free radical scavenger, the orphan of DPPH at place
Electronics is paired and it is made to fade, that is, is become smaller in the light absorption value of maximum absorption wave strong point.It therefore, can be by measuring extinction
The variation of value is to evaluate sample to the elimination effect of DPPH free radical.
DPPH radicals scavenging experiment specific experiment step be：
(1) prepare liquid and 2 × 10 of isometric (generally 3mL) is taken-4The DPPH solution of mol/L mixes (A1Pipe)；
(2) isometric dehydrated alcohol (determinand solvent) and 2 × 10 is taken-4The DPPH solution of mol/L mixes (A2Pipe)；
(3) isometric dehydrated alcohol and prepare liquid is taken to mix (A3Pipe)；
(4) after reacting 30min, A is surveyed at 517nm1、A2、A3Pipe absorbance value.
Clearance rate calculation formula is：Clearance rate (%)=[(A2+A3)-A1]/A2
Result as shown in Figure 1 it is found that 1 gained Semen Coicis extractive from fermentative of embodiment to the IC50=of DPPH scavenging effect
3.91%, illustrate that there is Semen Coicis extractive from fermentative stronger oxidation resistance can remove free radical, promote cell metabolism, enhancing is thin
Born of the same parents' vigor improves the structure and function of body, improves body vitality to delay cell senescence and plays the work of its anti-aging
With.
Embodiment 3
Tyrosinase is the key enzyme of melanogenesis, it controls the forming process of melanocyte, and level of activity is to pigment
Deposition plays a major role.Current market sales of many whitenings, nti-freckle product are all to inhibit tyrosinase to reach whitening and make
With, therefore be to evaluate the main indicator of whitening cosmetic to the power of tyrosinase inhibitory action.
The whitening function of sample is evaluated by measurement sample the influence of tyrosinase, and the specific method is as follows：
By the solution of proportion configuration shown in table 2：
Table 2, solution prepare list
| Unit (mL) | C1 | C2 | T1 | T2 |
| L-tyrosine | 2 | 2 | 2 | 2 |
| Sample | 0 | 0 | 2 | 2 |
| PBS | 4 | 5 | 2 | 3 |
| Tyrosinase | 1 | 0 | 1 | 0 |
| Total volume | 7 | 7 | 7 | 7 |
Note：C1And T11mL tyrosinase, enzyme activity 100U/mL is added.
(1)C2Pipe prepares shake up after, return to zero under the heating water bath 10min in 37 DEG C of water-baths, wavelength 475nm.
(2)C1Pipe solution, which prepares, to be shaken up, and after 37 DEG C of water-bath 10min, is added tyrosinase 1ml, is continued water-bath 10 minutes, measurement
C1Absorbance value.
(3) (1) (2) same method is pressed, with T2Zeroing measurement T1Absorbance value.
(4) sample is calculated to the maximum inhibition T (%) of tyrosinase.T (%)=(C1-T1)/c1× 100%
As shown in Fig. 2, experiments have shown that Semen Coicis extractive from fermentative is 64.53% to the average inhibition of tyrosinase activity,
1% arbutin is equivalent to the inhibiting rate 99.52% of tyrosinase activity, the i.e. whitening effect of Semen Coicis extractive from fermentative
The effect of 64.84% arbutin has certain white-skinned face function.
Embodiment 4
Patch test is mainly used for detecting the irritation of cosmetic end product or raw material.The present invention, which ferments to Semen Coicis, to be extracted
Materialization cosmetic carries out human closed formula patch test, assesses its potential skin irritation.
Suitable 30 people of volunteer is selected, the range of age was randomly choosed at 18-60 years old.Weighing 0.020g-0.025g will consolidate
Body sample or semisolid sample are put into spare in spot tester.Fluid sample 0.2mL to 0.025mL is added dropwise on filter paper,
Filter paper is placed in spot tester again.Each sample is respectively provided with blank control, is added and sample equivalent in control spot tester hole
Sample solvent, such as distilled water or olive oil.
Experiment position is selected as human body back, and spot tester fixation is pasted on subject back using nonirritant adhesive tape.
Test period continues for 24 hours.It is accurate, credible and scientific for test result, dduring test volunteer as requested, Bu Nengzhai
Fall spot tester, recipient site can not also be made to contact water.
It removes spot tester afterwards for 24 hours, after standing 30min, waits impression to disappear, observe the reaction of skin.If test result
For feminine gender, then need to observe again respectively with 48h for 24 hours after patch test primary.
Patch test results such as table 3：
Table 3, patch test results
* "-"=negative reaction；
" ± "=suspicious reaction：Only faint erythema；
"+"=weakly positive reaction (erythematous response)：Erythema, oedema, can have papule at infiltration；
" ++ "=strong positive reaction (bleb reaction)；Erythema, infiltration, oedema, papule, bleb；Reaction can exceed tested area；
" +++ "=extremely strong positive reaction (reaction of amalgamation bleb)；Obvious erythema, severe infiltration, oedema, amalgamation blister
Rash；Reaction exceeds tested area.
As shown in Table 3, the Semen Coicis extractive from fermentative that embodiment 1 provides does not generate in test adverse reaction.Through determining, the heart of a lotus seed
Rice extractive from fermentative has safety, will not bring security risk to human body.
Embodiment 5
The molecular size range of Semen Coicis extractive from fermentative detects
Molecular size range is to check whether an important factor for being able to carry out Cutaneous permeation.The present invention passes through high performance liquid chromatography
(HPLC) molecular size range of Semen Coicis extractive from fermentative prepared by measurement embodiment 1.
Specific step is as follows：
1, the preparation of the reagent and solution tested
(1) mobile phase：Acetonitrile: water: trifluoroacetic acid=30: 70: 0.1 (v/v/v).
(2) sample preparation：It is the sample of 5mg/ml by solvent compound concentration of mobile phase, then with microporous barrier (0.45 μm)
For sample introduction after filtering.
(3) prepared by standard sample：By bovine serum albumin (Mr 67000), B12 (Mr 1335), oxidized form of glutathione (Mr
614) it is made into mixed mark, the concentration of every kind of substance is 5mg/ml.
2, test method
By the standard items of three kinds of known molecular amounts：Bovine serum albumin(BSA) (Mw67000), vitamin B12(Mw1335) it and aoxidizes
Type glutathione (Mw614) passes through high performance liquid chromatograph (Waters company) reference literature " protease hydrolyzed wheat such as Wang Chengzhong
The research grain processing that polypeptide molecular weight is distributed in embryo egg bletilla enzymolysis liquid, 2012：Method in 37 (2) " carries out HPLC points
The standard curve of molecular weight logarithm and elution time is drawn in analysis.According to gel column permeation chromatography principle, the big object of polypeptide molecular weight
Matter first is eluted out, and the logarithm of elution time and molecular weight is in a linear relationship.It will be made in embodiment 1 according still further to same method
Standby Semen Coicis extractive from fermentative carries out HPLC analysis, obtains the elution time of respective peaks, elution time is substituted into standard curve, i.e.,
The molecular size range of the Semen Coicis extractive from fermentative of the preparation of embodiment 1 can be obtained.
Chromatographic condition：Chromatographic column：TsK gel 2000SWXL 300mm×7.8mm；Detection wavelength：UV 280nm；Flow velocity：
1ml/min；Column temperature：30℃.
3, test result
The molecular weight of standard substance and elution time it is as shown in table 4, figure is done according to the logarithm of molecular weight and elution time
Obtain standard curve：Y=-2.4738x+17.656, R2=0.9998.Wherein y is elution time (min)；X is molecular weight pair
Number.
Table 4, the molecular weight of standard substance and elution time
The HPLC map of Semen Coicis extractive from fermentative prepared by experimental group 1 is as shown in Figure 3：In the map of Semen Coicis extractive from fermentative
It mainly include 1 component peaks, elution time is substituted into standard curve by elution time 11.61min：Mono- 2.4738x+ of y=
17.656, the molecular weight for calculating appearance is 277.2Da, and accounts for 96.83% according to calculated by peak area Semen Coicis extractive from fermentative.One
As think that the active peptide components of molecular weight 500Da or so are more easily absorbed by the skin, be easy to be absorbed so existing in Semen Coicis fermentation material
Active peptides.
Three, the performance of keeping humidity detection of Semen Coicis extractive from fermentative
Semen Coicis extractive from fermentative prepared by embodiment 1 is as the cosmetics such as facial mask or Essence or toner directly to skin
Skin is smeared, and is detected by moisture content of skin testing experiment to its performance of keeping humidity, and specific step is as follows：
1, subjects
24 healthy trial volunteers are randomly selected, all subjects are without any skin and systemic disease history.And
The skin of recipient site (inner forearm) is without exception, does not also smear other external preparations such as any external drug and cosmetics.
2, test method
By tester (the Semen Coicis extractive from fermentative that experimental group, experimental group 2 and the experimental group 3 of embodiment 1 obtain) to tested
The skin of the inner forearm of person is smeared (amount of smearing is 0.08-0.1ml), and is control with distilled water.
Measure the skin of recipient site after 0h, 0.5h, 1h after smearing for Corneometer CM-825 using instrument
Water content, measurement result indicated by the humidity measurements (Moisture Measurement Val ue, MMV) of setting,
The numerical value that MMV is 0~150.Entire research is that temperature is 22 DEG C~24 DEG C indoors, the good skin that humidity is 50%~60%
It is carried out in noninvasive determination laboratory.
3, test result
Measurement result is as shown in Figure 4：It is Semen Coicis extractive from fermentative prepared by embodiment 1 in Fig. 4.It can from figure
It being compared out with control, after smearing the Semen Coicis extractive from fermentative that embodiment 1 obtains, moisture content of skin significantly improves subject,
And still there are very high moisture-keeping functions after smearing 1 hour.Illustrate that Semen Coicis extractive from fermentative of the invention has good moisturizing function
Can, it can be used for cosmetic in the water content for improving skin directly as facial mask or Essence or toner etc..
Claims (10)
1. a kind of preparation method of Semen Coicis extractive from fermentative, which is characterized in that include the following steps：
1) ingredient：Zymophyte：Semen Coicis powder：Water=5~10mL：10-15g, 250~350mL；The fermentation bacteria concentration is 105-
106CFU/ml；
2) initial system is prepared：The Semen Coicis powder, zymophyte, water are sufficiently mixed, initial system is obtained；
3) it ferments：Fermentation process is carried out to initial system, obtains fermentation liquid；
4) finished product is obtained：It successively sterilized to fermentation liquid, collect supernatant after centrifugal treating, obtained the Semen Coicis fermentation and extract
Object；
The zymophyte uses the bacterium solution of saccharomycete.
2. a kind of preparation method of Semen Coicis extractive from fermentative according to claim 1, which is characterized in that the saccharomycete is adopted
With red phaffia rhodozyma.
3. a kind of preparation method of Semen Coicis extractive from fermentative according to claim 1, which is characterized in that the Semen Coicis powder
Mesh number be 20-40 mesh.
4. a kind of preparation method of Semen Coicis extractive from fermentative according to claim 1, which is characterized in that the initial system
It is that zymogenic bacterium solution 8ml, 30 mesh coixlacrymajobi powder 10g and water 300ml is taken to be obtained by mixing.
5. a kind of preparation method of Semen Coicis extractive from fermentative according to claim 1, which is characterized in that sent out in the fermentation
Ferment temperature is 20-30 DEG C, and fermentation time is 35-40 hours.
6. a kind of preparation method of Semen Coicis extractive from fermentative according to claim 1, which is characterized in that the hair of the fermentation
Ferment temperature is 28 DEG C.
7. a kind of preparation method of Semen Coicis extractive from fermentative according to claim 1, which is characterized in that the centrifugal rotational speed
For 10000-12000r/min, centrifugation radius is 9-12cm；Centrifugation time is 15-30min.
8. a kind of Semen Coicis extractive from fermentative of any one of claim 1~7 preparation method preparation.
9. Semen Coicis extractive from fermentative answering in the cosmetics that preparation has following at least one functions according to claim 8
With or directly as application in the cosmetics with following at least one functions：
1) DPPH free radical is removed；
2) it inhibits tyrosinase activity；
3) moisturizing.
10. application according to claim 9, it is characterised in that：The cosmetics are facial mask, Essence or toner.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110403881A (en) * | 2019-08-29 | 2019-11-05 | 湖北省麦诗特生物科技有限公司 | A kind of preparation method and application of the river paddy seed tunning extract with white-skinned face function |
| CN114990186A (en) * | 2022-05-16 | 2022-09-02 | 广东完美生命健康科技研究院有限公司 | Coix seed multi-bacterium fermentation broth and preparation method and application thereof |
| CN115414313A (en) * | 2022-10-14 | 2022-12-02 | 佛山天韵化妆品科技有限公司 | Anti-aging composition containing micrococcus lysate and preparation method thereof |
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| CN114990186B (en) * | 2022-05-16 | 2024-02-09 | 广东完美生命健康科技研究院有限公司 | Coix seed multi-bacterium fermentation liquor and preparation method and application thereof |
| CN115414313A (en) * | 2022-10-14 | 2022-12-02 | 佛山天韵化妆品科技有限公司 | Anti-aging composition containing micrococcus lysate and preparation method thereof |
| CN115414313B (en) * | 2022-10-14 | 2023-11-14 | 佛山天韵化妆品科技有限公司 | Anti-aging composition containing micrococcus lysate and preparation method thereof |
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Application publication date: 20181123 |