CN105250169B - A kind of aloe proferment pulp cosmetic and the preparation method and application thereof - Google Patents

A kind of aloe proferment pulp cosmetic and the preparation method and application thereof Download PDF

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Publication number
CN105250169B
CN105250169B CN201510639589.4A CN201510639589A CN105250169B CN 105250169 B CN105250169 B CN 105250169B CN 201510639589 A CN201510639589 A CN 201510639589A CN 105250169 B CN105250169 B CN 105250169B
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aloe
magma
fermentation
zymophyte
preparation
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CN105250169A (en
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王昌涛
李萌
方国忠
方晓薇
赵丹
张佳婵
王冬冬
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SHANGHAI BIOTRULY BIOTECHNOLOGY Co.,Ltd.
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Shanghai Biotruly Biotechnology Co Ltd
Beijing Technology and Business University
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

Abstract

The invention discloses a kind of aloe proferment pulp cosmetic and the preparation method and application thereof.The preparation method of aloe fermentation magma provided by the invention, includes the following steps: for aloe magma, water and zymophyte to be mixed to get initial system, and carry out fermented and cultured to initial system, obtains the aloe fermentation magma.In the initial system, the zymophyte, the aloe magma and water ratio are as follows: (20~35) mL:(15~30) g:(250~400) mL；The zymogenic concentration is (106~109)CFU/mL.The zymophyte is streptococcus, specially streptococcus thermophilus.The present invention uses fermentation engineering, remains the active constituent in plant to the greatest extent, and avoid the introducing of organic solvent, safety and environmental protection with traditional water extraction relatively；The foreign substances such as addition enzyme are not needed, production cost is not only saved, but also maximumlly simplify production stage, so that the fermentation technique is can be realized mass production, industrialized production, and can fully ensure that the stability of product quality.

Description

A kind of aloe proferment pulp cosmetic and the preparation method and application thereof
Technical field
The invention belongs to field of biotechnology, and in particular to a kind of aloe proferment pulp cosmetic and preparation method thereof with answer With.
Background technique
Aloe (Aloe) is Liliaceae, perennial evergreen meat succulence herbaceous plant.Aloe be a kind of collection medicine food, health care, Beauty and watch in one Important Economic plant.The chemical component of aloe is considerably complicated, is studied for a long period of time and finds to contain in aloe There are 80 plurality of active ingredients headed by aloin.Mainly contain anthraquinone and its glucoside, glycosides, naphthalenone, resin, organic acid, carbohydrate, Protein, calcium oxalate, fiber, microelement etc..What aloe played a major role in terms of beauty and cosmetics is mucus ingredient, In polysaccharide material and the natural moisturizer of Amino acid profile, the moisture in skin can be supplemented, restore the function of collagen Can, facial wrinkles are prevented, skin smooth, high resilience are kept.Aloe arbutin, aloe crow in aloe is pungent (Aloeulcin) Gastric and duodenal ulcer can be treated, mucous membrane festers.Aloe arbutin can also prevent skin aging.China's Aloe resources are rich Richness provides advantage for development and application aloe.With luxuriant growth cultivation industry rapidly develop and application range widen, to aloe nutritional at Divide and studied in terms of medical care effect and is also gradually goed deep into.The extraction process of active aloe substance is also constantly improve, still Extracting method is mainly based on homogenate extraction method, water extraction.
What existing active aloe object extraction was commonly used is water extraction: aloe dry product adds deionized water, vacuum after crushing Filtrate is taken after suction filtration, filtrate is concentrated on a rotary evaporator, and last refrigerator cold-storage simultaneously uses ethanol precipitation, is produced after centrifugation Object.But this method introduces organic matter during the extraction process, not only breaks up environment, and there is potential peace during application Full hidden danger.Therefore, in order to avoid the above problem, it is desirable to provide a kind of to be mentioned using fermentation engineering to active aloe substance The method taken.
Summary of the invention
The object of the present invention is to provide a kind of aloe proferment pulp cosmetic and the preparation method and application thereof, this method is utilized Fermentation engineering extracts active material from aloe, easy to operate, and due to not adding any foreign substance, is prepared Aloe fermentate, that is, aloe fermentation magma has higher whitening and anti-aging activity to skin safe fanout free region.
The preparation method of aloe fermentation magma provided by the invention, includes the following steps: aloe magma, water and zymophyte It is mixed to get initial system, and fermented and cultured is carried out to initial system, obtains the aloe fermentation magma.
In above-mentioned preparation method, in the initial system, the proportion of the zymophyte, the aloe magma and water can be (18~35) mL:(15~30) g:(250~400) mL, the zymogenic concentration can be (106~109)CFU/mL；Specifically, In the initial system, the proportion of the zymophyte, the aloe magma and water can (18~35) mL:(15~30) g:320mL； More specifically, the proportion of the zymophyte, the aloe magma and water is 20mL:18g:320mL, institute in the initial system The concentration for stating aloe magma can be 106~109CFU/mL。
In above-mentioned preparation method, the temperature of the fermented and cultured can be 30~45 DEG C, concretely 35 DEG C；Time can be 24~38h, concretely 28h.
In above-mentioned preparation method, the zymophyte can be streptococcus, concretely streptococcus thermophilus Streptococcus thermophilus；The blade of the aloe magma from aloe, the slurry obtained after crushing for refiner, concretely exists The slurry like material that 3~10min is obtained is crushed under 1000~1200r/min revolving speed.
In above-mentioned preparation method, the zymophyte exists in the form of its culture solution or suspension；The zymophyte training The pH value of nutrient solution or the fermentation bacterium suspension can be 6.5~7.5, concretely 7.0.
In above-mentioned preparation method, the method further includes successively going out to fermentation liquid after the fermented and cultured Bacterium, the step of being centrifuged and taking supernatant.
In above-mentioned preparation method, the condition of the sterilizing is as follows: sterilising temp can be 115~121 DEG C, concretely 115℃；Sterilization time can be 15~25min, concretely 20min.
In above-mentioned preparation method, the condition of the centrifugation is as follows: centrifugal rotational speed can be 4000~6000r/min, specifically 4700r/min；The centrifugation time can be 20~30min, concretely 25min；The radius of the centrifugation can be 9cm.
Invention further provides a kind of aloe fermentation magma being prepared by above-mentioned preparation method.
Above-mentioned aloe fermentation magma has following 1) -4 in preparation) at least one of application in the cosmetics of function or Directly as with following 1) -4) at least one of application in the cosmetics of function, also within the scope of the present invention:
1) it inhibits tyrosinase activity；
2) skin-whitening；
3) DPPH free radical is removed；
4) anti-aging.
Above-mentioned 1) -4) application in, the cosmetics include but is not limited to: facial mask, Essence or toner.
The invention has the following beneficial effects:
(1) preparation method of aloe fermentate (i.e. aloe fermentation magma) provided by the invention uses fermentation engineering, By selecting suitable strain and fermentation condition, is extracted from aloe and obtain active material.On the one hand, it remains to the greatest extent Active constituent in plant, and the introducing of organic solvent, safety and environmental protection are avoided with traditional water extraction relatively；On the other hand, The foreign substances such as addition enzyme are not needed, production cost is not only saved, but also maximumlly simplify production stage, makes the fermentation skill Art can be realized mass production, industrialized production, and can fully ensure that the stability of product quality.
(2) in aloe provided by the invention fermentation magma containing the indispensable amino acid of healthy skin, minerals etc. from Right ingredient, and due to being not introduced into foreign substance during the preparation process, it, can be directly as facial mask or essence without any chemical component The finished product of magnificent liquid or toner uses, more natural than existing other products on the market, and any negative work will not be caused to skin With；And acquisition component molecules amount is small, is easy to that skin is allowed to fully absorb.
(3) aloe fermentation magma provided by the invention, there is stronger oxidation resistance can remove free radical, promote cell Metabolism enhances cell viability, improves the structure and function of body, improves body vitality, to delay cell senescence, has anti- The effect of aging.
(4) aloe fermentation magma provided by the invention, can inhibit the activity of tyrosinase, whitening effect is equivalent to The effect of 0.44% arbutin has certain white-skinned face function.
Detailed description of the invention
Fig. 1 is the active peptides molecular weight distribution HPLC map of aloe fermentation magma.
Fig. 2 is the relation curve of aloe the fermentation magma and DPPH free radical scavenging activity of various concentration.
The aloe that Fig. 3 is 10% ferment magma and 1% arbutin to the comparison diagram of the inhibiting rate of tyrosinase.
Specific embodiment
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Fluid nutrient medium in following embodiments is made of solute and solvent, and solvent is water, solute and its in Liquid Culture Concentration in base are as follows: glucose 20g/L, peptone 20g/L, yeast extract 10g/L, pH value 7.0.
YPD culture medium in following embodiments is made of solute and solvent, and solvent is water, solute and its liquid medium within In concentration are as follows: yeast extract 1% (mass fraction), tryptone 2% (mass fraction), glucose 2% (mass fraction), PH value is 7.0.
Aloe magma in following embodiments is that aloe leaf crushes in the refiner that revolving speed is 1000~1200r/min The slurry like material that 3~10min is obtained, aloe leaf used are sold from market.
The ATCC number of streptococcus thermophilus Streptococcus thermophilus in following embodiments is 19258, The public can be commercially available from ATCC.
Streptococcus thermophilus bacterium solution in following embodiments is made by the steps to obtain:
1, the activation of strain
One ring of picking streptococcus thermophilus (Streptococcus thermophilus) bacterium colony is put into fluid nutrient medium By actication of culture in shaking table, streptococcus thermophilus bacterium solution after being activated.
2, the purifying of strain
Bacterium solution gradient dilution bed board after the activation that step 1 is obtained obtains thermophilic chain after purification to obtain single colonie Coccus.
3, the expansion culture of strain
The strain after purification that step 2 is obtained is inoculated into YPD culture medium (pH value 7.0), in 25 DEG C of shaking table Culture, when value=0.6 OD~1.2, obtaining zymophyte bacterium solution, (strain is in logarithmic phase, concentration 106~109CFU/mL)。
Embodiment 1, preparation aloe fermentation magma
Aloe fermentation magma is prepared in accordance with the following steps
(1) by the streptococcus thermophilus fermentation bacterium bacterium solution that 20mL above-mentioned steps 3 obtain be inoculated into 18g aloe magma and In the water of 320mL, fermentation system is obtained；
(2) fermentation system is fermented 28 hours in 35 DEG C of shaking tables, obtains tunning；
(3) tunning is sterilized 20min at 115 DEG C, inactivates bacterium, the tunning after being sterilized；
(4) tunning after sterilizing is centrifuged 25min under conditions of 4700r/min, centrifugation radius are 9cm, it is heavy to abandon It forms sediment, collects supernatant, as aloe fermentation magma.
Embodiment 2, aloe fermentation magma are as the application in cosmetics
One, the property of aloe fermentation magma
Resulting fermentation magma prepared by embodiment 1 is used into active carbon decoloring, is de-tasted.Its appearance is thick liquid, face Color is faint yellow to brown color.PH value 5.2-6.3, viscosity 200-500cP, soluble solids content 1.5-5.0%, bacterium colony are total Number is less than 50CFU/mL, no pathogenic bacteria detection.According to hygienic standards for cosmetics GB7916-87, cosmetics total number of bacteria is not higher than 1000CFU/mL, so this fermentation magma meets cosmetics quality requirement.
Constituent analysis is carried out to aloe fermentation magma, protein detection method is referring to GB5009.5-2010；Thick many candies inspection Survey method is referring to GB/T 5009.8-2008；Flavones detection method is referring to GB/T 5009.124-2003；Total phenol detection method ginseng According to GB/T 8313-2008, acquired results are as follows:
Aloe fermentation magma 4.35g/kg containing protein, Thick many candies 15.36g/kg, general flavone produced by the present invention 0.086g/kg (in terms of rutin), total phenol 0.14g/kg.
Two, the safety detection of aloe fermentation magma
Human body patch test is mainly used for detecting the irritation of cosmetic end product or raw material.The present invention is to embodiment 1 The aloe fermentation magma of acquisition carries out human closed formula patch test, it is intended to assess its potential skin irritation.
1, subjects
Suitable 30 people of volunteer is selected, the range of age was randomly choosed at 18-60 years old.
2, test method
Weigh 0.020g-0.025g solid sample or semisolid sample be put into it is spare in spot tester.By fluid sample 0.2mL to 0.025mL dropwise addition is placed in spot tester on filter paper, then by filter paper.Each sample is respectively provided with blank control, The sample solvent being added in spot tester hole with sample equivalent is compareed, such as distilled water or olive oil (the present embodiment uses distilled water).
Experiment position is selected as human body back, and spot tester fixation is pasted on subject back using nonirritant adhesive tape. Test period continues for 24 hours.It is accurate, credible and scientific for test result, dduring test volunteer as requested, Bu Nengzhai Fall spot tester, recipient site can not also be made to contact water.It removes spot tester afterwards for 24 hours, after standing 30min, impression is waited to disappear, observation The reaction of skin.If test result is feminine gender, need to observe again respectively with 48h for 24 hours after patch test primary.
3, test result
Patch test results are as shown in table 1, and the meaning that each symbol indicates in table 1 is as follows: "-"=negative reaction；" ± "= Suspicious reaction: only faint erythema；"+"=weakly positive reaction (erythematous response): erythema, oedema, can have papule at infiltration；"++" =strong positive reaction (bleb reaction)；Erythema, infiltration, oedema, papule, bleb；Reaction can exceed tested area；" +++ "=it is extremely strong Positive reaction (reaction of amalgamation bleb)；Obvious erythema, severe infiltration, oedema, amalgamation bleb；Reaction exceeds tested area.
Judgment criteria: according to the number of 1 grade of skin adverse reaction of appearance in 2007,30 subjects of cosmetics health specification More than 5, or there is the number of second level skin adverse reaction more than 2, or any 1 three-level or the above skin of three-level occur not When good reaction, then determine that tested material has adverse reaction to human body, has no adverse reaction to human body conversely, being then considered as.
The patch test results for the aloe fermentation magma that table 1, embodiment 1 obtain
As can be seen from Table 1: the aloe fermentation magma that embodiment 1 obtains does not generate suspicious reaction, illustrates that the present invention mentions The aloe fermentation magma of confession all has safety, will not bring adverse reaction to human body.
Three, the molecular size range detection of aloe fermentation magma
Molecular size range is to check whether an important factor for being able to carry out Cutaneous permeation.The present invention passes through high performance liquid chromatography (HPLC) molecular size range of aloe fermentation magma prepared by measurement embodiment 1.Specific step is as follows:
1, the preparation of the reagent and solution tested
(1) mobile phase: acetonitrile: water: trifluoroacetic acid=30:70:0.1 (v/v/v).
(2) sample preparation: being the sample of 5mg/mL by solvent compound concentration of mobile phase, then with microporous barrier (0.45 μm) For sample introduction after filtering.
(3) prepared by standard sample: by bovine serum albumin (Mr 67000), B12 (Mr 1335), oxidized form of glutathione (Mr 614) it is made into mixed mark, the concentration of every kind of substance is 5mg/mL.
2, test method
By the standard items of three kinds of known molecular amounts: bovine serum albumin(BSA) (Mw67000), vitamin B12(Mw1335) it and aoxidizes Type glutathione (Mw614) passes through high performance liquid chromatograph (Waters company) reference literature " protease hydrolyzed wheat such as Wang Chengzhong Method in the research grain processing that polypeptide molecular weight is distributed in embryo egg bletilla enzymolysis liquid, 2012:37 (2) " carries out HPLC points The standard curve of molecular weight logarithm and elution time is drawn in analysis.According to gel column permeation chromatography principle, the big object of polypeptide molecular weight Matter first is eluted out, and the logarithm of elution time and molecular weight is in a linear relationship.It will be made in embodiment 1 according still further to same method Standby aloe fermentation magma carries out HPLC analysis, obtains the elution time of respective peaks, elution time is substituted into standard curve Obtain the molecular size range of the aloe fermentation magma of the preparation of embodiment 1.
Chromatographic condition: chromatographic column: TsK gel 2000SWXL 300mm × 7.8mm；Detection wavelength: UV 280nm；Flow velocity: 1ml/min；Column temperature: 30 DEG C.
3, test result
The molecular weight of standard substance and elution time it is as shown in table 2, figure is done according to the logarithm of molecular weight and elution time Obtain standard curve: y=-2.4738x+17.656, R2=0.9998.Wherein y is elution time (min)；X is molecular weight pair Number.
Table 2, the molecular weight of standard substance and elution time
The HPLC map of aloe fermentation magma prepared by embodiment 1 is as shown in Figure 1: from figure 1 it appears that aloe is fermented It mainly include 1 component peaks in the map of magma, elution time is substituted into standard curve: y=- by elution time 11.63min 2.4738x+17.656, the molecular weight for calculating appearance is 272.85Da, and is accounted for according to calculated by peak area aloe fermentation magma 99.95%.It is generally acknowledged that molecular weight 500Da or so activity peptide components are more easily absorbed by the skin, so existing in aloe fermentate Absorbable active peptides.
Four, the antioxygenic property detection of aloe fermentation magma
DPPH is a kind of organic free radical of early stage synthesis, is commonly used to the hydrogen supply capacity of assessment polyphenoils, it is organic It is highly stable in solvent, it is purple, and have a characteristic absorption peak, when encountering free radical scavenger, DPPH at 517nm Lone pair electrons be paired and it made to fade, that is, become smaller in the light absorption value of maximum absorption wave strong point.Therefore, measurement can be passed through The variation of light absorption value is to evaluate sample to the elimination effect of DPPH free radical.
The aloe fermentation magma for taking different amounts of embodiment 1 to be prepared is dissolved in deionized water, and it is dense that volume basis is made A series of aloes fermentation magma prepare liquid that degree is 0.63%, 1.25%, 2.50%, 5.00% and 10.00%.
The specific experiment step of DPPH radicals scavenging experiment are as follows:
(1) prepare liquid and 2 × 10 of isometric (generally 3mL) is taken-4The DPPH solution of mol/L mixes (A1Pipe)；
(2) isometric dehydrated alcohol (determinand solvent) and 2 × 10 is taken-4The DPPH solution of mol/L mixes (A2Pipe)；
(3) isometric dehydrated alcohol and prepare liquid is taken to mix (A3Pipe)；
(4) after reacting 30min, A is surveyed at 517nm1、A2、A3Pipe absorbance value.
Clearance rate calculation formula are as follows: clearance rate (%)=[(A2+A3)-A1]/A2 (1)
Using the concentration expressed in percentage by volume of aloe fermentation magma as abscissa, clearance rate is ordinate, production aloe fermentation magma To DPPH radicals scavenging effect curves, Fig. 2 is seen.
As seen from the figure, IC of 1 gained aloe of the embodiment fermentation magma to DPPH scavenging effect50=8.82%, illustrate aloe There is fermentation magma stronger oxidation resistance can remove free radical, promote cell metabolism, enhances cell viability, improves body Structure and function improves body vitality to delay cell senescence and plays the effect of its anti-aging.
Five, the white-skinned face function analysis of aloe fermentation magma
Tyrosinase is the key enzyme of melanogenesis, it controls the forming process of melanocyte, and level of activity is to pigment Deposition plays a major role.Current market sales of many whitenings, nti-freckle product are all to inhibit tyrosinase to reach whitening and make With, therefore be to evaluate the main indicator of whitening cosmetic to the power of tyrosinase inhibitory action.
The whitening function of sample is evaluated by measurement sample the influence of tyrosinase, and with 10%, (v/v, solvent are to go Ionized water) aloe fermentation magma be sample to be tested, measure its inhibitory effect to tyrosinase activity.
Table 2, solution prepare list
Solution C1(mL) C2(mL) T1(mL) T2(mL)
L-tyrosine 2 2 2 2
Sample 0 0 2 2
PBS 4 5 2 3
Tyrosinase 1 0 1 0
Total volume 7 7 7 7
Note: C1And T11mL tyrosinase, enzyme activity 100U/mL is added.
Each solution is configured by table 2, the maximum inhibition of tyrosinase in sample is measured in accordance with the following steps:
(1)C2Pipe prepares shake up after, return to zero under the heating water bath 10min in 37 DEG C of water-baths, wavelength 475nm.
(2)C1Pipe solution, which prepares, to be shaken up, and after 37 DEG C of water-bath 10min, is added tyrosinase 1mL, is continued water-bath 10 minutes, measurement C1Absorbance value.
(3) (1) (2) same method is pressed, with T2Zeroing measurement T1Absorbance value.
(4) sample is calculated to the maximum inhibition T (%) of tyrosinase.
The calculation formula of maximum inhibition T (%) are as follows: T (%)=(C1-T1)/C1× 100% (2)
It is demonstrated experimentally that 10% aloe is fermented, magma is 43.81% to the inhibiting rate of tyrosinase activity, 1% (v/v, solvent For deionized water) arbutin to the inhibiting rate 99.52% of tyrosinase activity, see Fig. 3.That is the whitening effect of aloe fermentation magma Fruit is equivalent to the effect of 0.44% arbutin, has certain white-skinned face function.

Claims (3)

1. a kind of aloe fermentation magma has following 1) -4 in preparation) at least one of application in the cosmetics of function or straight Connect as with following 1) -4) at least one of application in the cosmetics of function:
1) it inhibits tyrosinase activity；
2) skin-whitening；
3) DPPH free radical is removed；
4) anti-aging；
The aloe fermentation magma is prepared by the method included the following steps: aloe magma, water and zymophyte are mixed Conjunction obtains initial system, and carries out fermented and cultured to initial system, obtains the aloe fermentation magma；
The zymophyte, the aloe magma and water ratio are as follows: 20~35mL:15~30g:250~400mL；The fermentation The concentration of bacterium is 106~109CFU/mL；
The temperature of the fermented and cultured is 30~45 DEG C, and the time is 24~38h；
The zymophyte is streptococcus thermophilus Streptococcus thermophilus；The leaf of the aloe magma from aloe Piece；
The zymophyte exists in the form of its culture solution or suspension；The fermentation bacteria culture fluid or the fermentation bacterium suspension PH value be 6.5~7.5；
The method further includes the step for fermentation liquid successively being sterilized, being centrifuged and being taken supernatant after the fermented and cultured Suddenly.
2. application according to claim 1, it is characterised in that: the condition of the sterilizing is as follows: sterilising temp be 115~ 121 DEG C, sterilization time is 15~25min；
The condition of the centrifugation is as follows: centrifugal rotational speed is 3800~4200r/min, centrifugation time is 15~30min, is centrifuged radius For 9cm.
3. application according to claim 1, it is characterised in that: the cosmetics are facial mask, Essence or toner.
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CN105853303A (en) * 2016-05-30 2016-08-17 南昌大学 Preparation method of probiotic and aloe mask
CN108245464A (en) * 2018-02-28 2018-07-06 磐安县派普特生物科技有限公司 A kind of active aloe substance
CN109350584B (en) * 2018-10-17 2022-12-13 广州市孝真生物科技有限公司 Fermented raw juice of traditional Chinese medicine composition with powerful moisturizing effect and preparation and application thereof
CN111514077A (en) * 2020-04-02 2020-08-11 珠海中科先进技术研究院有限公司 Lactobacillus pentosus aloe fermented raw juice, preparation method, preservation method and application thereof
CN114788806A (en) * 2022-05-18 2022-07-26 加来(济南)生活科技有限公司 Traditional Chinese medicine composition fermented primary pulp with antioxidant and whitening integrated effects and preparation method and application thereof

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CN104382161A (en) * 2014-11-03 2015-03-04 南昌大学 Method for preparing streptococcus thermophilus ATCC 14485 and aloe co-fermented beverage

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104382161A (en) * 2014-11-03 2015-03-04 南昌大学 Method for preparing streptococcus thermophilus ATCC 14485 and aloe co-fermented beverage

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