CN109350648A - A kind of Fructus Forsythiae fermenation raw liquid and preparation method thereof - Google Patents
A kind of Fructus Forsythiae fermenation raw liquid and preparation method thereof Download PDFInfo
- Publication number
- CN109350648A CN109350648A CN201811180671.5A CN201811180671A CN109350648A CN 109350648 A CN109350648 A CN 109350648A CN 201811180671 A CN201811180671 A CN 201811180671A CN 109350648 A CN109350648 A CN 109350648A
- Authority
- CN
- China
- Prior art keywords
- fructus forsythiae
- fermenation raw
- raw liquid
- preparation
- bacterium solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
- A61K36/634—Forsythia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/04—Anorexiants; Antiobesity agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Child & Adolescent Psychology (AREA)
- General Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Obesity (AREA)
- Dermatology (AREA)
- Diabetes (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of Fructus Forsythiae fermenation raw liquid and preparation method thereof, the raw material for preparing of the fermenation raw liquid includes Fructus Forsythiae dry powder, water and bacterium solution.Fructus Forsythiae fermenation raw liquid preparation method carries out fermented and cultured the following steps are included: Fructus Forsythiae leaf dried powder, water and bacterium solution 1) are mixed to get initial system, to initial system, obtains Fructus Forsythiae fermentation liquid；2) after high-temperature sterilization, the membrane filtration for being 0.22 μm or 0.45 μm by fermentation liquid aperture removes impurity, obtains Fructus Forsythiae fermenation raw liquid.Method of the invention improves its medical value effectively by the extraction of effective component in Folium Forsythia；It is highly-safe, time-consuming is short, recovery rate is high, the pancreatic lipase activity component content that is inhibited simultaneously is high, good effect, the Folium Forsythia fermenation raw liquid that the present invention obtains, for liquid preparation fat reducing is made, to carry out smearing external application, human body is without sense of discomfort, and it can effectively solve the problem that the existing technological means for inhibiting obesity, solve the problems, such as drug to be leaned on or reduce food intake dose to be caused easily to cause people's digestive discomfort.
Description
Technical field
The present invention relates to technical field of plant extraction, it is specifically related to a kind of Fructus Forsythiae fermenation raw liquid and preparation method thereof.
Background technique
As the improvement of people's living standards, obesity has become a major global public health problem.According to statistics, at present
Existing 1,500,000,000 overweight patients in the whole world and 500,000,000 obese patients, and fat brought negative effect alreadys exceed excessive drinking and smoking.
Obesity is formally regarded as a kind of disease by June, 2013, American Medical Association.Obesity will lead to many related diseases, and such as II
Patients with type Ⅰ DM, cardiovascular disease, gallbladder disease, osteoarthritis, obstructive sleep apnea and cancer.
Pancreatic lipase (Pancreatic Lipase, PL) is by pancreas synthesis and secretion, is that hydrolysis dietary fat is main
Enzyme, it is responsible for the decomposition and digestion of 50%-70% dietary fat, and the fat in food is hydrolyzed to monoacylglycerol and free by PL
It after fatty acid, is absorbed in enteron aisle, then recombines fat in vivo again, caused fat accumulation, finally can lead to obesity.If
There are active principles to block decomposition of the PL to fat, and body will reduce the absorption of fat, so as to pre- preventing obesity or subtract
Light obese patient's weight.In recent years, since natural products has the characteristics that source is wide, at low cost, Small side effects, people is obtained and are got over
Carry out more concerns.It is also very extensive to the research of PL inhibitor in natural products, such as lotus leaf, radix scutellariae, buckwheat.
Fructus Forsythiae (Forsythia suspensa (Thunb.) Vahl) is Oleaceae forsythia, is distributed in China, Korea Spro
The states such as state and Japan.The ground such as China Shanxi, Henan, Shaanxi, Hebei are distributed.Civil, people often acquire Fructus Forsythiae tender leaf and add
Work is health tea use.Fructus Forsythiae fruit is 1 year knot, is influenced vulnerable to factors such as weathers, and Folium Forsythia is resourceful, and is bullied
The influence of the conditions such as time is smaller, thus more and more scholars start to focus on Folium Forsythia research.Folium Forsythia main component and Fructus Forsythiae phase
Seemingly, all there is liver protection, shield heart, antibacterial, anti-oxidant and anti-aging etc. comprising glycoside, flavonoids, phenylethanol, lignin etc.
Effect.2004, there is the discovery Folium Forsythiae extract such as Hou Gaixia effect for reducing blood fat (reducing blood lipid of Folium Forsythiae extract and to resist tired
Labor Effect study [D] 2004)；2018, all phenanthrene etc., which are studied, to be contained in Folium Forsythia more than total substance for inhibiting pancreatic lipase activity,
With antiobesity action (different Folium Forsythia processed products inhibit pancreatic lipase activity to compare [J] University Of Shanxi journal 2018).
The safety of active components of plants Yin Qigao and functional activity significantly penetrate into food, medicine, cosmetics etc.
Field.Therefore, the ingredient with high activity is extracted from plants using various extractive techniques and has become current research hotspot.
Traditional extractive technique includes organic solvent extraction, hot water extraction method, system solvent extraction method etc., but these method products are pacified
It is complete that property is low, time-consuming, recovery rate is low.The high and environmentally friendly Novel extraction method of highly-safe, recovery rate is increasingly closed by people
Note.It is a variety of enzymes secreted in process of production using microorganism that microbial method, which extracts active components of plants, thus in plant
Active constituent extract and modify.
Summary of the invention
It is an object of the invention to overcome the above problem and provide a kind of Fructus Forsythiae fermenation raw liquid and preparation method thereof, the system
Preparation Method, which obtains, inhibits pancreatic lipase activity ingredient high, good effect.
To achieve the above object, the technical scheme adopted by the invention is as follows:
The raw material for preparing of a kind of Fructus Forsythiae fermenation raw liquid, the fermenation raw liquid includes Fructus Forsythiae dry powder, water and bacterium solution.
It is a variety of enzymes secreted in process of production using microorganism that microbial method, which extracts active components of plants, thus to plant
Active constituent in object is extracted and is modified, and the present invention, which mixes Fructus Forsythiae with bacterium solution, ferments, to obtain the activity of Fructus Forsythiae
Ingredient, the results showed that, there is good inhibiting rate to PL using the Fructus Forsythiae fermentation liquid that method of the invention obtains, and pass through HPLC
Analysis obtains wherein containing to pancreatic lipase inhibiting effect Kaempferol-O- rutin sophorin, caffeic acid, forsythin A, and content is high,
It can effectively inhibit fat.
Preferably, the Fructus Forsythiae dry powder: bacterium solution: the proportion of water is (10~20) g:(5~10) ml:(250~300) ml.
Preferably, the Fructus Forsythiae dry powder, bacterium solution mass volume ratio are 15:10.Present inventor sends out by numerous studies
It is existing, it is best to pancreatic lipase activity inhibiting rate when bacterium solution is 10mL:15g with Fructus Forsythiae leaf dried powder adding proportion；When Folium Forsythia is dry
When the addition of powder amount is less, the effective component in fermentation liquid being likely to be obtained is less, and when its additive amount is more, company that may be excessive
The active constituent content decline that leaf dried powder inhibits the ferment effect of strain that fermentation is caused to obtain is stuck up, therefore the two is to pancreatic lipase
Inhibitory effect decline.
Preferably, the Fructus Forsythiae leaf dried powder, bacterium solution mass volume ratio are 15:(7.5~10).
Preferably, the Fructus Forsythiae dry powder is Fructus Forsythiae leaf dried powder.
Preferably, the preparation method of the bacterium solution is that 1) picking Lactococcus lactis bacterium colony, a ring are put into fluid nutrient medium
Lactococcus lactis by actication of culture in shaking table, after being activated；2) by the bacterium solution gradient dilution bed board after obtained activation, with
Just single colonie is obtained, Lactococcus lactis after purification is obtained；3) strain for obtaining after purification is inoculated into culture medium, at 25 DEG C
It is cultivated in shaking table, when value=0.5 OD~1.0, obtains zymophyte bacterium solution.
A kind of preparation method of Fructus Forsythiae fermenation raw liquid, comprising the following steps:
1) Fructus Forsythiae dry powder, water and bacterium solution are mixed to get initial system, fermented and cultured is carried out to initial system, obtains Fructus Forsythiae
Fermentation liquid；
2) after high-temperature sterilization, by fermentation liquid membrane filtration, impurity is removed, Fructus Forsythiae fermenation raw liquid is obtained；
Preferably, the step 1) ferments initial system 36~72 hours in 30~45 DEG C of shaking tables.
Preferably, the step 2) sterilizes tunning 15~30min at 80~110 DEG C, inactivates strain, obtains
Tunning after sterilizing；By the tunning after sterilizing in 3500r/min, it is centrifuged 10~15min, collects supernatant liquor, it will
The membrane filtration that fermentation liquid aperture is 0.22 or 0.45 μm removes impurity, obtains Fructus Forsythiae fermenation raw liquid.
Preferably, the bacterial concentration is (105~108)CFU/mL。
Preferably, the step 1) ferments initial system 36 hours in 45 DEG C of shaking tables.
Preferably, the step 2) sterilizes tunning 30min at 110 DEG C, inactivates strain, after being sterilized
Tunning；By the tunning after sterilizing in 3500r/min, it is centrifuged 10min, supernatant liquor is collected, by fermentation liquid aperture
For 0.22 or 0.45 μm of membrane filtration, impurity is removed, Fructus Forsythiae fermenation raw liquid is obtained.
Preferably, the fluid nutrient medium: 5.5% fructose is as carbon source, and 8% ammonium chloride is as nitrogen source, 2% yeast extract,
PH value is 7.0.
Preferably, the bacterial concentration is (105~108)CFU/mL；
Preferably, the pH value of the bacterium solution is 5.5~7.5.
Beneficial effects of the present invention: using method of the invention effectively by the extraction of effective component in Folium Forsythia, it is improved
Medical value；It is highly-safe, time-consuming is short, recovery rate is high, while the pancreatic lipase activity component content of being inhibited is high, good effect,
The Folium Forsythia fermenation raw liquid that the present invention obtains, for liquid preparation fat reducing is made, to carry out smearing external application, human body is without discomfort
Sense, and can effectively solve the problem that the existing technological means for inhibiting obesity, it is carried out mainly by drug or reduction food intake dose,
Which easily causes the problem of people's digestive discomfort.
Specific embodiment
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with specific embodiment to the present invention
It is described further.
The preparation method of Fructus Forsythiae leaf dried powder of the invention is high-temperature sterilization after washing with water Folium Forsythia completely, drying, powder
Broken, be sieved 50 mesh, and Fructus Forsythiae leaf dried powder is made.
Bacterium solution parameters of the present invention are as follows: strain is Lactococcus lactis；Bacterial concentration is 105~108CFU/mL；
The pH value of bacterium solution can be 5.5~7.5, concretely 6.0；Fermentation liquid cultivation temperature is 35~45 DEG C, and the time is 30~50h；Liquid
Body culture medium: 5.5% fructose is as carbon source, and 8% ammonium chloride is as nitrogen source, 2% yeast extract, pH value 7.0.
The preparation process of bacterium solution: picking Lactococcus lactis bacterium colony, a ring are put into shaking table strain is living in fluid nutrient medium
Change, the Lactococcus lactis after being activated.Bacterium solution gradient dilution bed board after obtained activation is obtained to obtain single colonie
To Lactococcus lactis after purification；The strain for obtaining after purification is inoculated into culture medium, is cultivated in 25 DEG C of shaking table, when OD value
When=0.5~1.0, obtaining zymophyte bacterium solution, (strain is in logarithmic phase, concentration 105~108CFU/ml)。
Embodiment 1
As a kind of a kind of embodiment of the preparation method of Fructus Forsythiae fermenation raw liquid of the present invention, the preparation method of the Fructus Forsythiae stoste
The following steps are included:
1) 10ml zymophyte bacterium solution is inoculated into the Fructus Forsythiae leaf dried powder of 10g and the water of 300ml, obtains fermentation system；It will
Fermentation system ferments 72 hours in 30 DEG C of shaking tables, obtains tunning.
2) tunning is sterilized 30min at 110 DEG C, inactivates strain, the tunning after being sterilized；It will sterilizing
Tunning afterwards is centrifuged 10min in 3500r/min, collects supernatant liquor, is taken out using 0.22 or 0.45 μm of filter membrane
Filter, obtaining filtrate is Fructus Forsythiae fermenation raw liquid.
Embodiment 2
As a kind of a kind of embodiment of the preparation method of Fructus Forsythiae fermenation raw liquid of the present invention, the preparation method of the Fructus Forsythiae stoste
The following steps are included:
1) 10mL zymophyte bacterium solution is inoculated into the Fructus Forsythiae leaf dried powder of 15g and the water of 300mL, obtains fermentation system；It will
Fermentation system ferments 36 hours in 45 DEG C of shaking tables, obtains tunning.
2) tunning is sterilized 30min at 110 DEG C, inactivates strain, the tunning after being sterilized；It will sterilizing
Tunning afterwards is centrifuged 10min in 3500r/min, collects supernatant liquor, is taken out using 0.22 or 0.45 μm of filter membrane
Filter, obtaining filtrate is Fructus Forsythiae fermenation raw liquid.
Embodiment 3
As a kind of a kind of embodiment of the preparation method of Fructus Forsythiae fermenation raw liquid of the present invention, the preparation method of the Fructus Forsythiae stoste
The following steps are included:
1) 10mL zymophyte bacterium solution is inoculated into the Fructus Forsythiae leaf dried powder of 20g and the water of 300mL, obtains fermentation system；It will
Fermentation system ferments 36 hours in 45 DEG C of shaking tables, obtains tunning.
2) tunning is sterilized 20min at 100 DEG C, inactivates strain, the tunning after being sterilized；It will sterilizing
Tunning afterwards is centrifuged 10min in 3500r/min, collects supernatant liquor, is filtered, obtained using 0.22 μm of filter membrane
Filtrate is Fructus Forsythiae fermenation raw liquid.
Embodiment 4
As a kind of a kind of embodiment of the preparation method of Fructus Forsythiae fermenation raw liquid of the present invention, the preparation method of the Fructus Forsythiae stoste
The following steps are included:
1) 5mL zymophyte bacterium solution is inoculated into the Fructus Forsythiae leaf dried powder of 10g and the water of 250mL, obtains fermentation system；It will hair
Ferment system is fermented 36 hours in 45 DEG C of shaking tables, obtains tunning.
2) tunning is sterilized 30min at 80 DEG C, inactivates strain, the tunning after being sterilized；It will sterilizing
Tunning afterwards is centrifuged 10min in 3500r/min, collects supernatant liquor, is filtered, obtained using 0.22 μm of filter membrane
Filtrate is Fructus Forsythiae fermenation raw liquid.
Embodiment 5
As a kind of a kind of embodiment of the preparation method of Fructus Forsythiae fermenation raw liquid of the present invention, the preparation method of the Fructus Forsythiae stoste
The following steps are included:
1) 7.5mL zymophyte bacterium solution is inoculated into the Fructus Forsythiae leaf dried powder of 15g and the water of 275mL, obtains fermentation system；It will
Fermentation system ferments 48 hours in 40 DEG C of shaking tables, obtains tunning.
2) tunning is sterilized 15min at 110 DEG C, inactivates strain, the tunning after being sterilized；It will sterilizing
Tunning afterwards is centrifuged 15min in 3500r/min, collects supernatant liquor, is taken out using 0.22 or 0.45 μm of filter membrane
Filter, obtaining filtrate is Fructus Forsythiae fermenation raw liquid.
Embodiment 6
Inhibitory enzyme activity measurement to Fructus Forsythiae fermenation raw liquid prepared by the present invention
The preparation of 1.Tris-HCl buffer solution
6.057g Tris-HCl accurately is weighed, then solution is transferred to pH=8.0 with HCl, is settled to 500mL with distilled water.
2. the preparation of Folium Forsythia fermentation liquid
Folium Forsythia fermenation raw liquid made from embodiment 1 to 5 is concentrated into thick, then is freeze-dried, obtains crude extract, it is standby
With.
It accurately weighs in dry Folium Forsythiae extract 0.1g and 1.5ml centrifuge tube, is dissolved in 1ml DMSO solution, it is sufficiently molten
After solution 1ml 0.1g/ml forsythia suspense extraction mother liquor, 20 μ L Folium Forsythiae extract solution are added in when experiment in the reaction system,
Obtaining concentration is 2000 μ g/ml crude extract solution.
The preparation of 3.PL solution
Accurately weigh 5mg PL, be dissolved in 10ml Tris-HCl (pH=8.0) buffer, vibrate, sufficiently dissolution to get
0.5mg/mL PL solution, it is ready-to-use.
The preparation of 4.PNPB solution
Accurately pipette 11 μ L of PNPB, add 5mL acetonitrile to get concentration be 12mmol PNPB solution, as substrate.
5.PL viability examination
It accurately pipettes in 100 μ l PL solution and 1.5ml centrifuge tube, 20 μ L Folium Forsythia crude extract solution is added, use
Tris-HCl buffer is settled to 900 μ l, sufficiently shakes up.People's enzyme solutions are not added as blank in reaction system, to be added without
Crude extract is as normal group.96 orifice plates are clicked and entered respectively, and each solution sets 4 multiple holes, is incubated for 15min at 37 DEG C, 20 μ are added immediately
LPNPB solution, is detected with microplate reader.Detection wavelength is 400nm, and every 3min measures a point, measures 15min, records number
According to calculating absorbance and change with time rate K.Inhibiting rate is calculated according to the following formula.Each reaction is in triplicate.
Inhibiting rate (%)=(normal group of-K experimental group of K)/K normally organizes * 100%
Test result is as follows table 1:
Table 1
As can be seen from the results: obtained fermenation raw liquid of the invention is to the good inhibiting rate of PL, when bacterium solution and Folium Forsythia
It is best to pancreatic lipase activity inhibiting rate when dry powder adding proportion is 10mL:15g；It, can when the addition of Fructus Forsythiae leaf dried powder amount is less
Effective component in getable fermentation liquid is less, and when its additive amount is more, may excessive Fructus Forsythiae leaf dried powder inhibit bacterium
The active constituent content decline that the ferment effect of kind causes fermentation to obtain, therefore the two declines the inhibitory effect of pancreatic lipase.
Embodiment 8
HPLC analysis is carried out to embodiment 1 to 5
Each ingredient in Fructus Forsythiae is Kaempferol-O- rutinoside, aurantiamarin, rutin, coffee to pancreatic lipase inhibiting effect
Coffee acid, forsythiaside A, the present invention carry out constituent analysis to fermenation raw liquid prepared by embodiment 1 to 5.
Folium Forsythia fermenation raw liquid obtained is concentrated into thick, then is freeze-dried, obtains crude extract, it is spare.
It takes 50mg Folium Forsythia crude extract in 1.5ml centrifuge tube, the oscillation of 40% ethanol solution of 1ml is added and mixes, uses needle
Solution is filled into vial by cartridge type membrane filter, carries out HPLC analysis.
HPLC testing conditions: being separated with C18 chromatographic column, Detection wavelength 270nm, and 25 DEG C of column temperature, flow velocity 0.8ml/
Min, mobile phase are methanol (A) and 0.3% aqueous acetic acid (B), gradient elution: 0~8min, 30%~33%A；8~
24min, 33%~40%A；24~39min, 40%~48%A；39~55min, 48%~64%A.
The ingredient contained in fermenation raw liquid is main are as follows: Kaempferol-O- rutin sophorin, forsythin, caffeic acid, forsythin A；
Each component content such as the following table 2 that different embodiments obtain:
Table 2
The result shows that ferment effect of the present invention is good, obtained Kaempferol-O- rutin sophorin, caffeic acid, forsythin A contains
Amount is high, wherein preferable with 2 ferment effect of embodiment.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent substitution, improvement and etc. done be should be included within the scope of the present invention.
Claims (10)
1. a kind of Fructus Forsythiae fermenation raw liquid, which is characterized in that the raw material for preparing of the fermenation raw liquid includes Fructus Forsythiae dry powder, water and bacterium
Liquid.
2. Fructus Forsythiae fermenation raw liquid according to claim 1, which is characterized in that the Fructus Forsythiae dry powder: bacterium solution: the proportion of water is
(10~20) g:(5~10) ml:(250~300) ml.
3. Fructus Forsythiae fermenation raw liquid according to claim 1, which is characterized in that the Fructus Forsythiae dry powder, bacterium solution mass volume ratio
For 15:10.
4. Fructus Forsythiae fermenation raw liquid according to claim 1, which is characterized in that the Fructus Forsythiae leaf dried powder, bacterium solution quality volume
Than for 15:(7.5~10).
5. Fructus Forsythiae fermenation raw liquid according to claim 1, which is characterized in that the Fructus Forsythiae dry powder is Fructus Forsythiae leaf dried powder.
6. Fructus Forsythiae fermenation raw liquid according to claim 1, which is characterized in that the preparation method of the bacterium solution is that 1) picking is newborn
Yogurt coccus falls, and a ring is put into shaking table the Lactococcus lactis by actication of culture, after being activated in fluid nutrient medium；2)
Bacterium solution gradient dilution bed board after obtained activation is obtained into Lactococcus lactis after purification to obtain single colonie；3) it will obtain
Strain after purification is inoculated into culture medium, is cultivated in 25 DEG C of shaking table, when value=0.5 OD~1.0, obtains zymophyte bacterium
Liquid.
7. a kind of preparation method such as Fructus Forsythiae fermenation raw liquid as claimed in any one of claims 1 to 6, which is characterized in that including with
Lower step:
1) Fructus Forsythiae dry powder, water and bacterium solution are mixed to get initial system, fermented and cultured is carried out to initial system, obtain Fructus Forsythiae fermentation
Liquid；
2) after high-temperature sterilization, by fermentation liquid membrane filtration, impurity is removed, Fructus Forsythiae fermenation raw liquid is obtained.
8. the preparation method of Fructus Forsythiae fermenation raw liquid according to claim 7, which is characterized in that the step 1) is by first initial body
It ties up in 30~45 DEG C of shaking tables and ferments 36~72 hours.
9. the preparation method of Fructus Forsythiae fermenation raw liquid according to claim 7, which is characterized in that the step 2) produces fermentation
Object sterilizes 15~30min at 80~110 DEG C, inactivates strain, the tunning after being sterilized；Fermentation after sterilizing is produced
Object is centrifuged 10~15min in 3500r/min, collects supernatant liquor, the filter membrane mistake for being 0.22 or 0.45 μm by fermentation liquid aperture
Filter removes impurity, obtains Fructus Forsythiae fermenation raw liquid.
10. the preparation method of Fructus Forsythiae fermenation raw liquid according to claim 7, which is characterized in that the bacterial concentration is (105
~108)CFU/mL。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811180671.5A CN109350648B (en) | 2018-10-10 | 2018-10-10 | Forsythia suspensa fermentation stock solution and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811180671.5A CN109350648B (en) | 2018-10-10 | 2018-10-10 | Forsythia suspensa fermentation stock solution and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109350648A true CN109350648A (en) | 2019-02-19 |
| CN109350648B CN109350648B (en) | 2021-07-30 |
Family
ID=65349037
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811180671.5A Active CN109350648B (en) | 2018-10-10 | 2018-10-10 | Forsythia suspensa fermentation stock solution and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109350648B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919463A (en) * | 2012-11-12 | 2013-02-13 | 左权县桐峪镇嘉百岁农民专业合作社 | Preparation method for fructus forsythiae leaf fermented tea |
| CN103719598A (en) * | 2013-12-19 | 2014-04-16 | 泰州中嘉农业开发有限公司 | Compound feed and preparing method thereof |
| CN104855684A (en) * | 2015-04-13 | 2015-08-26 | 成都乾坤动物药业有限公司 | An antibacterial traditional Chinese medicine microbial fermentation preparation and a preparation method and application thereof |
| CN106580789A (en) * | 2016-12-22 | 2017-04-26 | 北京工商大学 | Radix salviae miltiorrhizae fermented primary pulp cosmetic product as well as preparation method and applications thereof |
-
2018
- 2018-10-10 CN CN201811180671.5A patent/CN109350648B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919463A (en) * | 2012-11-12 | 2013-02-13 | 左权县桐峪镇嘉百岁农民专业合作社 | Preparation method for fructus forsythiae leaf fermented tea |
| CN103719598A (en) * | 2013-12-19 | 2014-04-16 | 泰州中嘉农业开发有限公司 | Compound feed and preparing method thereof |
| CN104855684A (en) * | 2015-04-13 | 2015-08-26 | 成都乾坤动物药业有限公司 | An antibacterial traditional Chinese medicine microbial fermentation preparation and a preparation method and application thereof |
| CN106580789A (en) * | 2016-12-22 | 2017-04-26 | 北京工商大学 | Radix salviae miltiorrhizae fermented primary pulp cosmetic product as well as preparation method and applications thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109350648B (en) | 2021-07-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105193876A (en) | Purslane extract and preparation method thereof | |
| CN106265846A (en) | A kind of fermentation process improving Radix Rhodiolae oxidation resistance | |
| CN106726949A (en) | A kind of grape pip proferment pulp cosmetic and preparation method and application | |
| CN103315359B (en) | Grifola frondosus solid state fermentation functional beverage and its preparation method | |
| CN101642450B (en) | New application of dicaffeoylquinic acid | |
| US20190000905A1 (en) | Gynostemma pentaphyllum fermented extracts comprising Saponins as an active functional food ingredient and preparation method thereof | |
| CN104257826A (en) | Traditional Chinese medicine pycnonotus sinensis fermented granule for livestock and poultry and preparation method of traditional Chinese medicine pycnonotus sinensis fermented granule | |
| WO2021093299A1 (en) | Astragalus-paecilomyces cicadae fermented fungal substance, preparation method and application thereof | |
| CN105998222A (en) | Probiotic fermented unprocessed common monkshood daughter root composition and preparation method and application thereof | |
| CN102764294A (en) | Cough relieving and sputum eliminating combination and preparation method thereof | |
| CN109350648A (en) | A kind of Fructus Forsythiae fermenation raw liquid and preparation method thereof | |
| CN103536710B (en) | The preparation method and application of Radix Ampelopsis extract | |
| CN101911988A (en) | Bamboo leaf health-care tea and preparation method thereof | |
| CN105585573B (en) | A kind of Tropolones compound and its pharmaceutical composition and its preparation method and application | |
| CN108201122A (en) | With the Cordyceps militaris composition and preparation method for improving intestinal microflora effect | |
| CN105125646B (en) | A kind of application of Trichoderma viride in improving maple knotweed prescription medicinal material in Quercetin recovery rate | |
| CN107334793A (en) | The purposes of wintersweet platymiscium helicobacter pylori resistant | |
| CN103263484A (en) | Fermentation method and fermentation product of tartary buckwheat bran | |
| CN101152202B (en) | Pharmaceutical composition for treating apparatus respiratorius disease and method for preparing the same | |
| CN103882075A (en) | Process for obtaining ginkgolide B by utilizing tremella aurantialba strains to transform ginkgo biloba extracts | |
| CN110507698A (en) | A kind of agent of common rabdosia leaf extract particles and preparation method of anti-mycobacterium tuberculosis | |
| CN109288902A (en) | A kind of preparation method and applications of the Wild jujube leaf total flavonoids tunning with strong anti-oxidative activity | |
| CN106860781A (en) | A kind of application of Rhizopus oryzae solid state fermentation extract, preparation method and its anticancer function | |
| CN113181229B (en) | Application of cabbage type rape-isatis tinctoria G monomer addition system in inhibiting novel coronavirus SARS-CoV-2 | |
| CN103655648B (en) | The preparation method of little leaf boxwood active component and purposes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |