CN103536710B - The preparation method and application of Radix Ampelopsis extract - Google Patents
The preparation method and application of Radix Ampelopsis extract Download PDFInfo
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- CN103536710B CN103536710B CN201210241868.1A CN201210241868A CN103536710B CN 103536710 B CN103536710 B CN 103536710B CN 201210241868 A CN201210241868 A CN 201210241868A CN 103536710 B CN103536710 B CN 103536710B
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Abstract
The invention discloses the preparation method of a kind of Radix Ampelopsis extract, the Radix Ampelopsis extract prepared by the method has moisture-keeping efficacy, it is possible to helps skin to resist in dry environments and is dried damage.Described Radix Ampelopsis extract can be additionally used in cosmetics additive, can moisturizing skin Caring after adding in cosmetics.The invention also discloses Radix Ampelopsis extract and prepare the purposes in the cosmetics of skin moisture-keeping.
Description
Technical field
The present invention relates to field of natural medicinal chemistry and cosmetic field, be specifically related to a kind of for moisturizing
The preparation method and applications of Chinese herbal medicine Radix Ampelopsis.
Background technology
Air humidity reduces, and keratodermatitis can not regulate enough Moisture factor in time, and the work of oils and fats gland
The ability that jumps also is lowering, and oils and fats on the face will reduce, and therefore skin is easy for tightening, and even wrinkles
Stricture of vagina, especially now and more there will be microgroove by nose.Moisture-retention function of skin be skin nursing class cosmetics and
The most basic function of skin care product, is also one of skin problem of paying close attention to the most of cosmetics research staff.
Wind, be dried, in the environment of cold, skin loss moisture, skin becomes to be dried, follows the string,
Wrinkling, acceleration skin aging, damages to skin, causes being dried damage.Protection under dry environment is right
An important step it is probably for skin moisture-keeping.The wetting agent of R and D excellent performance is that people are diligent
In the hope of target.
China's Chinese medicine aboundresources, finds superior moisturizing additive for us and provides the foundation.Radix Ampelopsis,
Have another name called mountain region melon, wild Radix Ipomoeae, Vitis Amurensis seedling, Bletilla striata (Thunb.ex A.Murray)Rchb.f., Herba Rubi blinii etc., being dried for vitaceae Radix Ampelopsis
Tuber.It is distributed in North China, northeast, East China, the Central-South and ground such as Shaanxi, Ningxia, Sichuan.Spring, Qiu Erji
Excavate, remove silt and radicula, be cut into vertical lobe or inclined tab, dry.Can be with heat-clearing and toxic substances removing, eliminating carbuncle eliminating stagnation.
Additionally, be different from the most common external calculating hydroscopicity and moisturizing rate to evaluate additive moisture retention
Method, present invention employs a kind of new method for evaluating in vitro moisture-keeping efficacy.
Summary of the invention
It is an object of the invention to provide a kind of Chinese herbal medicine Radix Ampelopsis with moisture-keeping function, by joining of providing
This Radix Ampelopsis extract that side and preparation method are made, can be directly used for skin moisture-keeping, it is possible to as merit
Effect additive joins in cosmetics and uses, and cost is relatively low.
Radix Ampelopsis extract prepared according to the methods of the invention has moisture-keeping efficacy, it is possible to help skin dry
Resist under dry environment and be dried damage.Radix Ampelopsis extract is used for cosmetics, there is the effect of moisturizing skin Caring,
Skin brilliance is made to moisten, non-stimulated to skin.
It is an object of the invention to provide the preparation method of a kind of Radix Ampelopsis extract, described method includes using
Solvent extraction, suitable solvent includes acetone, water, such as distilled water, deionized water and pure water,
The alcoholic solution of variable concentrations, the polyhydric alcohol solutions of variable concentrations, the solvent that other polarity are similar, or
It it is the combination of above-mentioned solvent.
Preferably, the step of described solvent extraction is selected from: decoction, reflux, extract, dipping, percolation or
A combination thereof.
Preferably, described preparation method also includes: filter extraction fluid and residue, it is thus achieved that extracting solution；
Concentrate alternatively；Dissolve alternatively.
Preferably, described preparation method also includes: filter extraction fluid and residue, it is thus achieved that extracting solution；
Concentrate alternatively；Precipitate with ethanol；Filter precipitation separation；Dissolution precipitation；Add preservative.Described preservative
Selected from Kazon, extremely U.S.-PLUS or a combination thereof.
It is a further object to provide the Radix Ampelopsis extraction that preparation in accordance with the present invention obtains
Thing.
The Radix Ampelopsis extract obtained prepared according to the methods of the invention can be directly used for skin moisture-keeping, it is also possible to
Join in cosmetics as functional additive and use.Preferably, Radix Ampelopsis extract can be according to
0.01-50%(weight ratio) ratio be added in cosmetics.Preferably, that adds in cosmetics is white
The consumption of liana extract can be 0.02-20%, more preferably 0.05-10%, most preferably 0.1-5%.
Therefore, a further object of the present invention is to provide Radix Ampelopsis extract in the cosmetics of preparation moisturizing
Purposes.Preferably, described Radix Ampelopsis extract is that the Radix Ampelopsis that the method according to the invention prepares carries
Take thing.
A further object of the present invention is to extract the cosmetics of a kind of moisturizing, its comprise Radix Ampelopsis extract with
And the acceptable adjuvant of cosmetic field.Preferably, described Radix Ampelopsis extract is the side according to the present invention
The Radix Ampelopsis extract that method prepares.
Preferably, described cosmetics can be beautifying and skin-protecting cream, eye cream, facial film, cleansing milk, soft skin
Water, essence.
Radix Ampelopsis has another name called mountain region melon, wild Radix Ipomoeae, Vitis Amurensis seedling, Bletilla striata (Thunb.ex A.Murray)Rchb.f., Herba Rubi blinii etc., plants for Vitaceae
The dried root of thing Radix Ampelopsis.Spring, season in autumn two excavate, and remove silt and radicula, are cut into vertical lobe or inclined tab,
Dry.Can be with heat-clearing and toxic substances removing, eliminating carbuncle eliminating stagnation.Radix Ampelopsis comes from " herbal classic ".TAO Hong-Jing: Radix Ampelopsis shortcut
There is it everywhere.Make rattan raw, the root such as Radix Angelicae Dahuricae.Fragmentation, wears it with bamboo, and day does.Life takes root and smashes deposited carbuncle
Also imitate.
The Radix Ampelopsis extract that the method for the present invention prepares has moisture-keeping efficacy, it is possible to give skin angle
The good moisture-keeping function of cell plastid, it is possible to help skin to resist in dry environments and be dried damage, help
Skin keeps moisture.After adding in cosmetics, there is the effect of moisturizing skin Caring, make skin brilliance moisten
Pool, non-stimulated to skin.
Detailed description of the invention
Present inventors discovered unexpectedly that: Radix Ampelopsis extract has moisture-keeping efficacy, can be directly used for skin
Moisturizing and resist drying damage, it is possible to join in cosmetics as functional additive and use.According to institute of the present invention
State Radix Ampelopsis extraction solution and cosmetics that method prepares, it is possible to help skin to resist in dry environments and be dried damage
Wound, moisturizing skin Caring, make skin brilliance moisten, non-stimulated to skin.
Radix Ampelopsis, has another name called mountain region melon, wild Radix Ipomoeae, Vitis Amurensis seedling, Bletilla striata (Thunb.ex A.Murray)Rchb.f., Herba Rubi blinii etc., for vitaceae
The dried root of Radix Ampelopsis.It is distributed in North China, northeast, East China, the Central-South and ground such as Shaanxi, Ningxia, Sichuan.
Spring, season in autumn two excavate, and remove silt and radicula, are cut into vertical lobe or inclined tab, dry.Can with heat-clearing and toxic substances removing,
Eliminating carbuncle eliminating stagnation.
The modern study of Radix Ampelopsis is concentrated mainly on its antifungic action.Such as, water logging agent (1:3) is in examination
To Trichophyton concentricunm, Du big belly microspore tinea bacterium difficult to understand, epidermophyton inguinale and epidermophyton rubrum etc. in pipe
Dermatophytes all has inhibitory action in various degree.Clinical report, Radix Ampelopsis can be used for treating bacillary dysentery,
Radix Ampelopsis tuber dries or dries, pulverization, loads capsule, treats acute bacillary dysentery.Can also be used for therapeutic
Purulence dermatosis, Radix Ampelopsis tuber, peeling grinds to the powder, with boiling water stirring agglomerating after, add ethanol furnishing thick paste,
External application affected part, to more.
All being not directed to Radix Ampelopsis extract application in cosmetics in document, not mentioned Radix Ampelopsis extract has guarantor
Wet effect.Therefore, present invention firstly provides Radix Ampelopsis and there is moisture-keeping efficacy, can add as functional additive
In the cosmetics of moisturizing, help skin to resist in dry environments and be dried damage, moisturizing skin Caring.
The invention provides the consumption proportion of various formula:
Radix Ampelopsis 100
Preservative qs
When producing, technological process is as follows:
Taking Radix Ampelopsis medical material appropriate, soak about 48h with appropriate vehicle, extract with correlation method, filtrate adds suitable
Preferably solvent, filters, resolution of precipitate, adds appropriate polyhydric alcohol and preservative, make containing crude drug amount 50-1500mg/ml
Solution.
Appropriate vehicle includes water (distilled water, deionized water, pure water), the alcoholic solution of variable concentrations, no
With the solvent that the polyhydric alcohol solutions of concentration and other polarity are similar；Extracting method can be decoction, refluxing carries
Take, impregnate, the method such as percolation；Preservative can be Kazon, extremely U.S.-plus etc..
Can be by Radix Ampelopsis extract according to 0.01 ~ 50%(weight ratio) ratio be added in cosmetics, make up
Product are selected from beautifying and skin-protecting cream, eye cream, facial film, cleansing milk, toner/smoothing toner, essence etc..
The present invention is expanded on further below in conjunction with specific embodiment.It should be appreciated, however, that these are real
Execute example to be merely to illustrate the present invention and be not meant to limit the scope of the invention.The following example is not noted
The test method of bright actual conditions, generally according to normal condition, or according to the bar proposed by manufacturer
Part.Except as otherwise noted, all of percentage ratio and number are by mass.
Embodiment 1
Take pure Chinese crude drug Radix Ampelopsis appropriate, add deionization water boiling and extraction twice, each 1 hour, filter
Crossing, extract obtained water dissolution, making containing crude drug amount is the solution of 1000mg/ml.
Embodiment 2
Take pure Chinese crude drug Radix Ampelopsis appropriate, add deionization water boiling and extraction twice, each 1 hour, filter
Cross, concentrate, add 3 times amount ethanol precipitate with ethanol, stand；Filter, precipitation water dissolution, add appropriate
Liquid extremely U.S.-plus, making containing crude drug amount is the solution of 1000mg/ml.
Embodiment 3
Taking pure Chinese crude drug Radix Ampelopsis appropriate, add 75% soak with ethanol 48h, filter, residue uses decocting again
Boil extraction about twice, concentrate, add 3 times amount ethanol precipitate with ethanol, stand；Filter, precipitation water dissolution,
Adding appropriate amount of fluid extremely U.S.-plus, making containing crude drug amount is the solution of 1000mg/ml.
Embodiment 4
Taking pure Chinese crude drug Radix Ampelopsis appropriate, add acetone soak 48h, filter, acetone is removed in evaporation, and medical material is residual
Slag by water boiling and extraction about twice, concentrates, adds 3 times amount ethanol precipitate with ethanol again, stands；Filter, heavy
Shallow lake water dissolution, adds appropriate amount of fluid extremely U.S.-plus, and making containing crude drug amount is the solution of 1000mg/ml.
Embodiment 5
Take pure Chinese crude drug Radix Ampelopsis appropriate, add 80 degree of extractions of 10% aqueous solution of propylene glycol twice, each 1
Hour, filtering, extract obtained water dissolution, making containing crude drug amount is the solution of 1000mg/ml.
Embodiment 6: the evaluation of external moisture-keeping efficacy
1.1 test material
Cell: people epidermal keratinocyte strain HaCat
Reagent: MEM culture medium, 0.25% pancreatin, calf serum, dual anti-, PBS(phosphoric acid buffer
Liquid), MTT
Material and instrument: 24 porocyte culture plates, CO2Incubator, inverted microscope, microplate reader,
Drimeter
1.2 test method
1.2.1 cell is cultivated
People's epidermal keratinocyte is normally cultivated with the MEM culture medium containing 10% calf serum, works as cell
Grow to fusion rate when reaching more than 80%, remove culture medium, wash once with PBS, be subsequently adding 0.25%
Trypsinization, when cell shrinkage becomes round, incline pancreatin, adds containing the MEM of 10% calf serum
Blow and beat gently, collect cell and be centrifuged, count cell, with MEM, cell concentration is adjusted to suitably
Concentration is seeded in culture bottle.To be grown to fusion rate reach more than 80% time, pass on next time.
1.2.2 cell synchronization
Cell grows to fusion rate when reaching more than 80%, adds the MEM culture medium without calf serum
Make synchronization process 12h.
1.2.3 plating cells
Cell after synchronization, 0.25% trypsinization, collect cell counting, adjust cell concentration, connect
Planting to 24 well culture plates, the every hole of 1ml cell suspension, 5%CO2,37 DEG C of overnight incubation make adherent.
1.2.4 dosing
Treat cell attachment, 1%, 0.5% and 0.1% that after 24h, addition cell proliferation has no significant effect
The Radix Ampelopsis extract of concentration, sets up Normal group (not making dried group), blank respectively
Group (dried blank group) and dosing group, often the multiple hole of group 3, hatches 24h.
1.2.5 dried
At 25 DEG C, in super-clean bench, it is dried wind speed 0.3m/s-0.5m/s, RH%=45% ± 5%, blank
Matched group and the every hole of dosing group suck all culture medium successively, place 10min ~ 15min in super-clean bench.
1.2.6MTT measure
Cell after dried continues normally to cultivate 24h with the MEM culture medium containing 10% calf serum
After, every hole adds MTT liquid (0.5mg/ml) 500ul, 5%CO2, 37 DEG C hatch 4h after go to 96
Orifice plate, 570nm measures.
1.2.7 interpretation:
Recording each hole resist drying time (T), often average in the multiple hole of group 3, and experimental result is applied
Statistical software STATA8.0 analyzes, and carries out the statistical procedures such as t inspection, variance analysis.P < 0.05,
Represent significant difference；P < 0.01 represents difference highly significant.And it is dry to calculate cell according to below equation
Dry mortality rate (%) and protection ratio (%).
Cell is dried mortality rate (%)=[1-(blank/medicine) OD value/normal blank OD value] * 100%；
Protection ratio (%)=[(blank group cell is dried mortality rate-medicine group cell and is dried mortality rate)/empty
White group cell is dried mortality rate] * 100%.
Result is as follows:
| Radix Ampelopsis extract | 1% | 0.5% | 0.1% |
| Protection ratio (%) | 35.87%** | 30.60%* | 15.25%* |
*: relative to blank group, have pole significant difference (p < 0.01)
*: relative to blank group, have significant difference (p < 0.05).
#: blank group, for Normal group, is formed and is dried damage, p < 0.01, have
Pole significant difference.
As can be seen here, Radix Ampelopsis extract is dried damage for skin can be protected to resist in dry environments,
There is effect of moisturizing skin Caring.
Claims (6)
1. Radix Ampelopsis extract purposes in the cosmetics preparing resist drying damage, described Radix Ampelopsis extract is pressed
Preparing according to following methods: solvent extraction, described solvent is selected from: water, acetone, alcohol, and other polarity are similar
Solvent, or the combination of above-mentioned solvent, the step of described solvent extraction is selected from: decoction, reflux, extract, dipping,
Percolation or a combination thereof.
2. purposes as claimed in claim 1, described method also includes:
Filter extraction fluid and residue, it is thus achieved that extracting solution；
Concentrate alternatively；
Dissolve alternatively.
3. purposes as claimed in claim 1, described method also includes:
Filter extraction fluid and residue, it is thus achieved that extracting solution；
Concentrate alternatively；
Precipitate with ethanol；
Filter precipitation separation；
Dissolution precipitation；
Add preservative.
4. purposes as claimed in claim 3, it is characterised in that described preservative is selected from Kazon, extremely U.S.
-PLUS or a combination thereof.
5. purposes as claimed in claim 1, described toiletry bag is containing Radix Ampelopsis extract and cosmetics neck
The acceptable adjuvant in territory.
6. purposes as claimed in claim 5, described cosmetics can be beautifying and skin-protecting cream, eye cream, face
Film, cleansing milk, toner/smoothing toner, essence.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210241868.1A CN103536710B (en) | 2012-07-12 | 2012-07-12 | The preparation method and application of Radix Ampelopsis extract |
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| CN201210241868.1A CN103536710B (en) | 2012-07-12 | 2012-07-12 | The preparation method and application of Radix Ampelopsis extract |
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| CN105147881A (en) * | 2015-10-28 | 2015-12-16 | 李琳 | Compound menthol balm for treating burns |
| CN109984973A (en) * | 2019-05-09 | 2019-07-09 | 李媛媛 | A kind of acne-eliminating cosmetic and preparation method thereof containing compound plant extract |
| CN113082128B (en) * | 2021-04-12 | 2022-05-03 | 南方医科大学 | Ampelopsis japonica extract, preparation method thereof and application of ampelopsis japonica extract in preventing and treating dengue fever type II virus infection |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0665039A (en) * | 1992-06-05 | 1994-03-08 | Nonogawa Shoji Kk | Cosmetic |
| JPH07126149A (en) * | 1993-10-28 | 1995-05-16 | Suntory Ltd | Cosmetic composition for beautifying and whitening |
| JP2002212087A (en) * | 2001-01-22 | 2002-07-31 | Kose Corp | Skin care preparation |
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2012
- 2012-07-12 CN CN201210241868.1A patent/CN103536710B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0665039A (en) * | 1992-06-05 | 1994-03-08 | Nonogawa Shoji Kk | Cosmetic |
| JPH07126149A (en) * | 1993-10-28 | 1995-05-16 | Suntory Ltd | Cosmetic composition for beautifying and whitening |
| JP2002212087A (en) * | 2001-01-22 | 2002-07-31 | Kose Corp | Skin care preparation |
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