CN103536462A - Preparation method and application of bletilla striata extract - Google Patents
Preparation method and application of bletilla striata extract Download PDFInfo
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- CN103536462A CN103536462A CN201210241612.0A CN201210241612A CN103536462A CN 103536462 A CN103536462 A CN 103536462A CN 201210241612 A CN201210241612 A CN 201210241612A CN 103536462 A CN103536462 A CN 103536462A
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Abstract
The invention discloses a preparation method of a bletilla striata extract. The bletilla striata extract prepared by the method has a dry damage resistance function and can be helpful for skins to resist dry damage under a dry environment. The bletilla striata extract further can be used for additives of cosmetics, and further has the dry damage resistance function after being added into cosmetics. The invention further discloses use of the bletilla striata extract in preparing cosmetics for preventing dry damages.
Description
Technical field
The present invention relates to Natural Medicine Chemistry field and cosmetic field, be specifically related to preparation method and the application thereof of a kind of Chinese herbal medicine Pseudobulbus Bletillae (Rhizoma Bletillae) for resist drying damage.
Background technology
Air humidity reduces, and keratodermatitis can not regulate in time, and the ability of enlivening of oils and fats gland is also lowering, and oils and fats on the face just can reduce, so skin just easily tightens, and wrinkle occurs even, especially now and the other microgroove that more there will be of nose.
Under wind, dry, cold environment, skin loss moisture, that skin becomes is dry, follow the string, wrinkling, accelerate skin aging, to skin, damage, cause dry damage.Protection under dry environment can not be ignored for skin, and it is significant that research and development have the resist drying damage additive of good effect.
China's Chinese medicine aboundresources, provides the foundation for we find superior resist drying damage additive.The Pseudobulbus Bletillae (Rhizoma Bletillae) is a kind of of the orchid family Pseudobulbus Bletillae (Rhizoma Bletillae) genus.Perennial herb, high 20～50 centimetres, 4～5 pieces, leaf, base portion mutually cover builds up stem shape, and central authorities extract scape out.A raceme tool number flower; Flower purple or pale red, 5 centimetres of diameters, are comprised of the lip of 3 pieces of sepals, 2 pieces of petals and 1 piece of specialization; Lip 3 splits, and has typhloscle sheet above; Stamen and style symphysis form gynostemium, and there is a flower pesticide on gynostemium top, and there is a stigma depression front upper place.Underground have a thick thick root stock, as Caput Gallus domesticus shape, and rich viscosity, containing Pseudobulbus Bletillae (Rhizoma Bletillae) colloid, i.e. bletilla mannan, can hyoscine, has the effect of hemostasis tonifying the lung, granulation promoting pain relieving, also can be made for thickener.Pseudobulbus Bletillae (Rhizoma Bletillae) genus has 6 kinds, all originates in East Asia.
In addition, the present invention has adopted a kind of new method for external assessment resist drying damage.
Summary of the invention
The object of the present invention is to provide a kind of can resist drying the Chinese herbal medicine Pseudobulbus Bletillae (Rhizoma Bletillae) of damage, this Pseudobulbus Bletillae (Rhizoma Bletillae) extract of making by the formula providing and preparation method, can be directly used in skin resist drying damage, also can be used as functional additive and join in cosmetics and use, and cost be lower.
Pseudobulbus Bletillae (Rhizoma Bletillae) extract prepared according to the methods of the invention can damage by resist drying, can help skin under dry environment, to resist dry damage.Pseudobulbus Bletillae (Rhizoma Bletillae) extract, for cosmetics, is had to the effect of resist drying damage equally.
The object of the present invention is to provide a kind of preparation method of Pseudobulbus Bletillae (Rhizoma Bletillae) extract, described method comprises with solvent extraction, suitable solvent comprises acetone, water, for example distilled water, deionized water and pure water, the alcoholic solution of variable concentrations, the polyhydric alcohol solutions of variable concentrations, solvent like other polar phase, or the combination of above-mentioned solvent.
Preferably, the step of described solvent extraction is selected from: decoction, reflux, extract,, dipping, percolation or its combination.
Preferably, described preparation method also comprises: isolated by filtration extracting solution and residue, obtain extracting solution; Concentrated alternatively; Dissolve alternatively.
Preferably, described preparation method also comprises: isolated by filtration extracting solution and residue, obtain extracting solution; Concentrated alternatively; Precipitate with ethanol; Isolated by filtration precipitation; Dissolution precipitation; Add antiseptic.Described antiseptic is selected from Kazon, extremely U.S.-PLUS or its combination.
Another object of the present invention is to provide the Pseudobulbus Bletillae (Rhizoma Bletillae) extract that preparation in accordance with the present invention obtains.
The Pseudobulbus Bletillae (Rhizoma Bletillae) extract obtaining prepared according to the methods of the invention can be directly used in skin resist drying damage, also can be used as functional additive and joins in cosmetics and use.Preferably, Pseudobulbus Bletillae (Rhizoma Bletillae) extract can be according to 0.01-50%(weight ratio) ratio be added in cosmetics.Preferably, the consumption that adds the Pseudobulbus Bletillae (Rhizoma Bletillae) extract in cosmetics to can be 0.02-20%, more preferably 0.05-10%, most preferably 0.1-5%.
Therefore, another object of the present invention is to provide the purposes of Pseudobulbus Bletillae (Rhizoma Bletillae) extract in the cosmetics of preparing resist drying damage.Preferably, described Pseudobulbus Bletillae (Rhizoma Bletillae) extract is the Pseudobulbus Bletillae (Rhizoma Bletillae) extract that the method according to this invention prepares.
Another object of the present invention is to extract a kind of cosmetics of resist drying damage, and it comprises Pseudobulbus Bletillae (Rhizoma Bletillae) extract and the acceptable adjuvant of cosmetic field.Preferably, described Pseudobulbus Bletillae (Rhizoma Bletillae) extract is the Pseudobulbus Bletillae (Rhizoma Bletillae) extract that the method according to this invention prepares.
Preferably, described cosmetics can be beautifying and skin-protecting cream, eye cream, facial film, cleansing milk, toner/smoothing toner, essence.
The Pseudobulbus Bletillae (Rhizoma Bletillae) extract that method of the present invention prepares has the effect of resist drying damage, can help skin under dry environment, to resist dry damage.After adding in cosmetics, there is equally the effect of resist drying damage.
The specific embodiment
The present inventor finds after having passed through extensive and deep research: Pseudobulbus Bletillae (Rhizoma Bletillae) extract has resist drying damaging action, can be directly used in skin resist drying damage, also can be used as functional additive and joins in cosmetics and use.The Rhizoma Bletillae extraction solution making according to the method for the invention and cosmetics, can help skin under dry environment, to resist dry damage, makes skin honorable moist.
The Pseudobulbus Bletillae (Rhizoma Bletillae) is the tuber of the orchid Pseudobulbus Bletillae (Rhizoma Bletillae), tonifying the lung, hemostasis, detumescence, granulation promoting, sore.Control lung and hinder hemoptysis, epistaxis, incised wound is hemorrhage, swollen ulcer drug, ulcer pain, soup fire is burnt, rhagadia manus et pedis.< < property of medicine opinion > >: " control stagnation of pathogenic heat and do not disappear, flaccidity under main the moon, controls Shao-bleb on face, makes us flesh and slides." < < Tang materia medica > >: " brothers' chap is torn open, chews to be coated with it."
In document, all do not relate to the effect that Pseudobulbus Bletillae (Rhizoma Bletillae) extract has resist drying damage, also the functional additive of the not mentioned damage using it as resist drying adds in cosmetics.Therefore, the present invention proposes the effect that the Pseudobulbus Bletillae (Rhizoma Bletillae) has resist drying damage first, can be used as in the cosmetics that functional additive adds resist drying damage, helps skin under dry environment, to resist dry damage.
The invention provides the consumption proportion of various formulas:
The Pseudobulbus Bletillae (Rhizoma Bletillae) 100
Antiseptic is appropriate
When producing, technological process is as follows:
Get Pseudobulbus Bletillae (Rhizoma Bletillae) medical material appropriate, with suitable solvent, soak about 48h, with correlation method, extract, filtrate adds suitable solvent, filters, and resolution of precipitate, adds appropriate polyhydric alcohol and antiseptic, makes the solution containing crude drug amount 50-1500mg/ml.
Suitable solvent comprises water (distilled water, deionized water, pure water), the alcoholic solution of variable concentrations, solvent like the polyhydric alcohol solutions of variable concentrations and other polar phase; Extracting method can be the methods such as decoction, reflux, extract,, dipping, percolation; Antiseptic can be Kazon, extremely U.S.-plus etc.
Can be by Pseudobulbus Bletillae (Rhizoma Bletillae) extract according to 0.01 ~ 50%(weight ratio) ratio be added in cosmetics, cosmetics can be selected from beautifying and skin-protecting cream, eye cream, facial film, cleansing milk, toner/smoothing toner, essence etc.
Below in conjunction with specific embodiment, further set forth the present invention.But, should be understood that these embodiment only do not form limitation of the scope of the invention for the present invention is described.The test method of unreceipted actual conditions in the following example, conventionally according to normal condition, or the condition of advising according to manufacturer.Except as otherwise noted, all percentage ratio and umber are by mass.
Embodiment 1
Get the pure Chinese crude drug Pseudobulbus Bletillae (Rhizoma Bletillae) appropriate, add deionization water boiling and extraction twice, each 1 hour, filter, extract obtained with water dissolution, the solution that to make containing crude drug amount be 1000mg/ml.
Embodiment 2
Get the pure Chinese crude drug Pseudobulbus Bletillae (Rhizoma Bletillae) appropriate, add deionization water boiling and extraction twice, each 1 hour, filter, concentrated, add 3 times of amount ethanol precipitate with ethanol, standing; Filter, precipitation water dissolution, adds extremely U.S.-plus of appropriate amount of fluid, the solution that to make containing crude drug amount be 1000mg/ml.
Embodiment 3
Get the pure Chinese crude drug Pseudobulbus Bletillae (Rhizoma Bletillae) appropriate, add 75% soak with ethanol 48h, filter, residue is again by water boiling and extraction approximately twice, concentrated, adds 3 times of amount ethanol precipitate with ethanol, standing; Filter, precipitation water dissolution, adds extremely U.S.-plus of appropriate amount of fluid, the solution that to make containing crude drug amount be 1000mg/ml.
Embodiment 4
Get the pure Chinese crude drug Pseudobulbus Bletillae (Rhizoma Bletillae) appropriate, add acetone and soak 48h, filter, acetone is removed in evaporation, and medical material residue is again by water boiling and extraction approximately twice, concentrated, adds 3 times of amount ethanol precipitate with ethanol, standing; Filter, precipitation water dissolution, adds extremely U.S.-plus of appropriate amount of fluid, the solution that to make containing crude drug amount be 1000mg/ml.
Embodiment 5
Get the pure Chinese crude drug Pseudobulbus Bletillae (Rhizoma Bletillae) appropriate, add 10% aqueous solution of propylene glycol 80 degree and extract twice, each 1 hour, filter, extract obtained with water dissolution, the solution that to make containing crude drug amount be 1000mg/ml.
Embodiment 6: the evaluation of the dry damage of In Vitro Anti effect
1.1 test material
Cell: people's epidermal keratinocyte strain HaCat
Reagent: MEM culture medium, 0.25% pancreatin, calf serum, dual anti-, PBS(phosphate buffer), MTT
Material and instrument: 24 porocyte culture plates, CO
2incubator, inverted microscope, microplate reader, drimeter
1.2 test method
1.2.1 cell culture
People's epidermal keratinocyte is normally cultivated with the MEM culture medium containing 10% calf serum, when Growth of Cells to fusion rate reaches 80% when above, remove culture medium, with PBS, wash once, then add 0.25% trypsinization, when cell shrinkage becomes bowlder, the pancreatin that inclines, adds the MEM containing 10% calf serum to blow and beat gently, and collecting cell is also centrifugal, counting cells, is adjusted to debita spissitudo with MEM by cell concentration and is seeded in culture bottle.Wait growing to fusion rate, reach 80% when above, go down to posterity next time.
1.2.2 cell synchronization
Growth of Cells to fusion rate reaches 80% when above, does not add and makes synchronization process 12h containing the MEM culture medium of calf serum.
1.2.3 cell bed board
Cell after synchronization, 0.25% trypsinization, collecting cell counting, adjusts cell concentration, is seeded to 24 well culture plates, the every hole of 1ml cell suspension, 5%CO2,37 ℃ of overnight incubation make adherent.
1.2.4 dosing
Treat cell attachment, the Pseudobulbus Bletillae (Rhizoma Bletillae) extract that adds 1%, 0.5% and 0.1% concentration that on cell proliferation has no significant effect after 24h, set up respectively Normal group (not making dried group), blank group (the blank group of dried) and dosing group, 3 every group multiple holes, hatch 24h.
1.2.5 dried
At 25 ℃, in super-clean bench, dry wind speed 0.3m/s-0.5m/s, RH%=45% ± 5%, blank group and the every hole of dosing group suck all culture medium successively, place 10min ~ 15min in super-clean bench.
1.2.6 MTT measures
Cell after dried continues after the normal 24h of cultivation with the MEM culture medium containing 10% calf serum, and every hole adds MTT liquid (0.5mg/ml) 500ul, 5%CO
2, 37 ℃ go to 96 orifice plates after hatching 4h, and 570nm measures.
1.2.7 interpretation:
Recording each hole resist drying time (T), averages in 3 every group multiple holes, and experimental result applied statistics software STATA 8.0 analyzes, and carries out the statistical procedures such as t check, variance analysis.P<0.05, represents significant difference; P<0.01 represents difference highly significant.And calculate the dry mortality rate (%) of cell and protection ratio (%) according to following formula.
Cell is dried mortality rate (%)=[1-(blank/medicine) OD value/normal blank OD value] * 100%;
Protection ratio (%)=[(the dry mortality rate of the dry mortality rate-medicine group cell of blank group cell)/dry mortality rate of blank group cell] * 100%.
Result is as follows:
| Pseudobulbus Bletillae (Rhizoma Bletillae) extract | 1% | 0.5% | 0.1% |
| Protection ratio (%) | 35.87% ** | 30.60% * | 15.25% * |
*: with respect to blank group, there is utmost point significant difference (p<0.01)
*: with respect to blank group, there is significant difference (p<0.05).
#: blank group, for Normal group, forms dry damage, and p<0.01, has utmost point significant difference.
As can be seen here, Pseudobulbus Bletillae (Rhizoma Bletillae) extract has the effect of resist drying damage, can protect skin under dry environment, to resist dry damage.
Claims (10)
1. a preparation method for Pseudobulbus Bletillae (Rhizoma Bletillae) extract, described method comprises:
Solvent extraction, described solvent is selected from: water, acetone, alcohol, polyhydric alcohol, solvent like other polar phase, or the combination of above-mentioned solvent.
2. preparation method as claimed in claim 1, is characterized in that, the step of described solvent extraction is selected from: decoction, reflux, extract,, dipping, percolation or its combination.
3. preparation method as claimed in claim 1 or 2, described method also comprises:
Isolated by filtration extracting solution and residue, obtain extracting solution;
Concentrated alternatively;
Dissolve alternatively.
4. preparation method as claimed in claim 1 or 2, described method also comprises:
Isolated by filtration extracting solution and residue, obtain extracting solution;
Concentrated alternatively;
Precipitate with ethanol;
Isolated by filtration precipitation;
Dissolution precipitation;
Add antiseptic.
5. preparation method as claimed in claim 4, is characterized in that, described antiseptic is selected from: Kazon, extremely U.S.-PLUS or its combination.
6. the Pseudobulbus Bletillae (Rhizoma Bletillae) extract preparing according to method described in any one in claim 1-5.
7. the purposes of Pseudobulbus Bletillae (Rhizoma Bletillae) extract in the cosmetics for the preparation of resist drying damage.
8. purposes as claimed in claim 7, is characterized in that, described Pseudobulbus Bletillae (Rhizoma Bletillae) extract is the extract of preparing according to method described in any one in claim 1-5.
9. the cosmetics that resist drying damages, it comprises Pseudobulbus Bletillae (Rhizoma Bletillae) extract and the acceptable adjuvant of cosmetic field.
10. cosmetics as claimed in claim 9, described cosmetics can be beautifying and skin-protecting cream, eye cream, facial film, cleansing milk, toner/smoothing toner, essence.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210241612.0A CN103536462A (en) | 2012-07-12 | 2012-07-12 | Preparation method and application of bletilla striata extract |
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| CN201210241612.0A CN103536462A (en) | 2012-07-12 | 2012-07-12 | Preparation method and application of bletilla striata extract |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105816507A (en) * | 2016-04-20 | 2016-08-03 | 中国科学院武汉植物园 | Bletilla striata callus skin care preparation and preparation method thereof |
| CN106137844A (en) * | 2016-08-11 | 2016-11-23 | 史加桃 | A kind of infant diaper ointment and preparation method thereof |
| CN106833977A (en) * | 2016-12-15 | 2017-06-13 | 湖南利诺生物药业有限公司 | Citral dendrobium candidum perfumed soap and preparation method thereof |
| CN106880568A (en) * | 2017-03-08 | 2017-06-23 | 张仕英 | A kind of ultrasonic technology extracts the preparation method of facial mask bletilla striata extract |
| CN107669583A (en) * | 2017-09-30 | 2018-02-09 | 上海清轩生物科技有限公司 | A kind of moisture-keeping composition and its application |
| CN108210423A (en) * | 2018-04-18 | 2018-06-29 | 王风云 | It is a kind of for ingredient of moisturizing emulsion and preparation method thereof |
| CN109846720A (en) * | 2019-01-22 | 2019-06-07 | 湖北中医药大学 | A kind of tear-pull bletilla whitening mask and preparation method thereof |
| CN113304088A (en) * | 2021-05-26 | 2021-08-27 | 上海辉文生物技术股份有限公司 | Preparation method of bletilla striata extract with repairing and whitening effects and bletilla striata extract |
| CN114848568A (en) * | 2022-06-01 | 2022-08-05 | 广州富煜陇生物科技有限公司 | Compound plant extract with moisturizing effect and preparation and application thereof |
| CN115957140A (en) * | 2021-10-13 | 2023-04-14 | 香港城市大学 | Water-soluble one-way moisture-conducting traditional Chinese medicine mask and preparation method thereof |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105816507A (en) * | 2016-04-20 | 2016-08-03 | 中国科学院武汉植物园 | Bletilla striata callus skin care preparation and preparation method thereof |
| CN106137844A (en) * | 2016-08-11 | 2016-11-23 | 史加桃 | A kind of infant diaper ointment and preparation method thereof |
| CN106833977A (en) * | 2016-12-15 | 2017-06-13 | 湖南利诺生物药业有限公司 | Citral dendrobium candidum perfumed soap and preparation method thereof |
| CN106880568A (en) * | 2017-03-08 | 2017-06-23 | 张仕英 | A kind of ultrasonic technology extracts the preparation method of facial mask bletilla striata extract |
| CN107669583B (en) * | 2017-09-30 | 2020-08-04 | 上海清轩生物科技有限公司 | Moisturizing composition and application thereof |
| CN107669583A (en) * | 2017-09-30 | 2018-02-09 | 上海清轩生物科技有限公司 | A kind of moisture-keeping composition and its application |
| CN108210423A (en) * | 2018-04-18 | 2018-06-29 | 王风云 | It is a kind of for ingredient of moisturizing emulsion and preparation method thereof |
| CN109846720A (en) * | 2019-01-22 | 2019-06-07 | 湖北中医药大学 | A kind of tear-pull bletilla whitening mask and preparation method thereof |
| CN109846720B (en) * | 2019-01-22 | 2021-11-16 | 湖北中医药大学 | Tear-type bletilla striata whitening mask and preparation method thereof |
| CN113304088A (en) * | 2021-05-26 | 2021-08-27 | 上海辉文生物技术股份有限公司 | Preparation method of bletilla striata extract with repairing and whitening effects and bletilla striata extract |
| CN115957140A (en) * | 2021-10-13 | 2023-04-14 | 香港城市大学 | Water-soluble one-way moisture-conducting traditional Chinese medicine mask and preparation method thereof |
| CN114848568A (en) * | 2022-06-01 | 2022-08-05 | 广州富煜陇生物科技有限公司 | Compound plant extract with moisturizing effect and preparation and application thereof |
| CN114848568B (en) * | 2022-06-01 | 2023-09-01 | 广州富煜陇生物科技有限公司 | Compound plant extract with moisturizing effect and preparation and application thereof |
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Application publication date: 20140129 |