CN105777933B - The preparation of algal polysaccharides and its application in cosmetics - Google Patents
The preparation of algal polysaccharides and its application in cosmetics Download PDFInfo
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- CN105777933B CN105777933B CN201610118886.9A CN201610118886A CN105777933B CN 105777933 B CN105777933 B CN 105777933B CN 201610118886 A CN201610118886 A CN 201610118886A CN 105777933 B CN105777933 B CN 105777933B
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/001—Preparations for care of the lips
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0084—Guluromannuronans, e.g. alginic acid, i.e. D-mannuronic acid and D-guluronic acid units linked with alternating alpha- and beta-1,4-glycosidic bonds; Derivatives thereof, e.g. alginates
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Abstract
A kind of preparation the present invention provides algal polysaccharides and its application in cosmetics.The preparation method of the algal polysaccharides is：It cleans after seaweed is crushed through methanol, hot acid extraction method is recycled to extract wherein acid-soluble polysaccharide after the abundant methanol removal of residue, then the relatively low macromolecular polysaccharide constituents of bioavailability are removed by alcohol precipitation, after surplus solution recycling ethyl alcohol, dialysis, freeze-drying, obtains algal polysaccharides.The molecular weight of gained algal polysaccharides is 8 35kDa, has notable antioxidation and moisturizing activity, available for cosmetic field.Skin cream prepared therefrom for skin surfaces such as hand, face, necks, has effects that moist moisturizing, mitigates wrinkle, slows down aging.Eye cream prepared therefrom for circumference of eyes, has effects that moist moisturizing, mitigates wrinkle, slows down aging.
Description
Technical field
The present invention relates to cosmetic field, the preparation more particularly, to a kind of algal polysaccharides and its answering in cosmetics
With.
Background technology
Seaweed is submarine algae, is the cryptogam of plant kingdom, and algae includes several inhomogeneities with photosynthetic work
With the biology for generating energy.They are generally considered to be simple plant, are mainly characterized by：Without vascular tissue, without real
Root, stem, the differentiating phenomenon of leaf；It does not bloom, no fruit and seed；Protective tissue of the reproductive organs without specialization, often directly by single
Cell generates spore or gamete；And the formation without embryo.The general designation of marine products algae (Algae), usually it is solid write in seabed or certain
It is single plant or a lot of simple plant that basal cell is formed on kind solid structure.The biology run after fame with seaweed enumerates
Many kinds, these body differences are huge, across a variety of life entities, and common ground is mainly lived in the seawater, can by from
The in vivo chromatoplast of body and photosynthesis carry out synthesis of organic substance.
Sargassum fusifome, a kind of algae, for one kind in seaweed, alias deer horn point, edible seaweed bud, goat breast, extra large barley etc..
Frond yellowish-brown, plump succulence, thallophytic variation is very big, and shape is various.It is grown on low tide band rock, in Chinese state
North gets Liaodong Peninsula, there is its distribution in south to the Lezhou Peninsula；It is most with Along Zhejiang Coast.
Seaweed, which is used as medicine, has history remote in China, written by Southern Qi Dynasty's Tao Hongjing (Christian era 452~536)《Sheng Nong's herbal classic》
On describe seaweed and describe dietotherapy property and Application way.Simultaneously also reducing blood lipid, antithrombotic and eliminate brain fag,
Promote child development, promote immunity of organisms, slow down aging and other effects.
Sargassum fusifome is Sargassaceae sea-plant, drying frond seaweed is included by Chinese Pharmacopoeia, containing there are many into
Divide such as polysaccharide, protein, polyphenol, minerals and various trace elements, there is radioresistance, antitumor, immunological regulation, antioxygen
Change and other effects.Application of its extract in cosmetics gradually increases in recent years, has moisturizing, sun-proof, adjusting sebum secretion, prolongs
The effects that slow aging.And Hijiki polysaccharide has antitumor action, there is the effect of promoting hematopoiesis function and enhancing immune function, it can
It prevents thrombosis, reduce cholesterol and prevents hypertension.
In the prior art, the moisturizing activity of extract, patent are focused mainly on to the extraction process of Hijiki polysaccharide
A kind of extraction process of sargassum fusifome active polysaccharide is disclosed in CN101250232A, is carried with reference to water, alkali carries, hot water carry and ultrasonic wave
Technology extracts sargassum fusifome active polysaccharide.But in this method, the only extraction stage just employs first normal-temperature water and carries, then at ice-bath ultrasonic ripple
Reason, then 70-80 DEG C of water carries, the method finally extracted again with buck, there are process it is numerous and diverse the problem of.
The content of the invention
For in the prior art the defects of, the object of the present invention is to provide a kind of preparation of algal polysaccharides and its in cosmetics
In application.The molecular weight of gained algal polysaccharides is 8-35kDa, is acid-soluble polysaccharide, has notable antioxidation and moisturizing
Activity, suitable for preparing moisturizing class and anti-aging class cosmetics.
The purpose of the present invention is what is be achieved through the following technical solutions：
The present invention provides a kind of preparation method of algal polysaccharides, the preparation method is：Through methanol after seaweed is crushed
It cleans, after the abundant methanol removal of residue hot acid extraction method is recycled to extract wherein acid-soluble polysaccharide, then removed by ethanol precipitation
After the macromolecular polysaccharide constituents that bioavailability is relatively low, surplus solution is gone to recycle ethyl alcohol, membrane filtration, freeze-drying is divided
The algal polysaccharides of son amount 8-35kDa.
The seaweed includes sargassum fusifome.
Preferably, the preparation method is as follows：
Removal of impurities：By the seaweed of dries pulverizing with methanol eddy extraction 2-4 it is small when, filtering, filtrate recycling methanol；Filter residue volatilizes
Residual methanol, it is fully dry at 45-50 DEG C, it is spare；
Extraction：Dried filter residue is extracted with hydrochloric acid solution, Extracting temperature is 70-75 DEG C, when extraction 2-4 is small,
Filtering, filtrate is evaporated under vacuum, obtains semifluid shape crude extract；
Alcohol precipitation：The crude extract obtained by extraction step is soluble in water, ethyl alcohol is added, after stirring evenly, stands 24-
48 it is small when, filtering, filtrate removal ethyl alcohol after, it is spare；
Dialysis：Gained filtrate after alcohol precipitation is used into algal polysaccharides of the membrane filter method molecular cut off for 8-35kDa, is concentrated,
Freeze-drying to get.
The optimum extraction temperature of the present invention is at 70-75 DEG C, if temperature is too low, recovery rate is not high；Temperature is excessively high, gained polysaccharide
Extract performance of keeping humidity reduces.
Preferably, in the removal step：The solid-to-liquid ratio of seaweed and methanol is that the methanol of 20-30mL is corresponded to per 1g seaweed,
I.e. 1:20-1:30(g/mL).
Preferably, in the extraction step, the molar concentration of hydrochloric acid solution is 0.1-0.2M；The material of filter residue and hydrochloric acid solution
Liquor ratio is that the hydrochloric acid solution of 15-30mL is corresponded to per 1g filter residues, i.e., 1：15-1:30(g/mL)；Vacuum condition is vacuum degree 0.085-
0.098Mpa.The concentration of hydrochloric acid is too low, antioxygenic property can be caused to reduce；Excessive concentration can cause performance of keeping humidity to reduce.Material
Hydrochloric acid solution is very few in liquor ratio antioxygenic property to be caused to reduce, and hydrochloric acid solution excessively increases subsequent concentrated cost.
Preferably, in the alcohol precipitation step, the volumetric concentration of the ethyl alcohol of addition in water is 30-50%.
A kind of use of the algal polysaccharides obtained the present invention also provides preparation method of the algal polysaccharides in cosmetics
On the way.
The present invention also provides a kind of skin lotion containing algal polysaccharides, in terms of mass fraction 100%, including following quality point
Several each components：Foregoing algal polysaccharides 1-3%, aloe polysaccharide extracting solution 10-20%, silicone grease 5-10%, caprylic/capric glycerine
Three ester 3-6%, jojoba oil 3-6%, octadecyl alcolol 2-5%, Brij72 polyoxyethylene stearyl base ether 2-4%, Brij721 polyoxy second
Alkene stearyl ether 2-4%, butanediol 3-5%, Acritamer 940 0.2-0.5%, Phenoxyethanol 0.5-0.8%, ethylhexyl are sweet
Oily 0.5-0.8%, EDETATE SODIUM 0.05-0.1%, triethanolamine 0.2-0.4%, essence 0.3-0.8%, surplus are deionization
Water.
The present invention also provides a kind of preparation methods of the skin lotion containing algal polysaccharides, comprise the following steps：
S1, each component raw material is weighed：
Oil phase：Silicone grease, caprylic/capric triglyceride, jojoba oil, octadecyl alcolol, Brij72 polyoxyethylene stearyl base ethers 2,
Brij721 polyoxyethylene stearyl base ethers；
Water phase：Algal polysaccharides, aloe polysaccharide extracting solution, butanediol, Acritamer 940, EDETATE SODIUM, deionized water；
Afterwards plus phase：Essence, Phenoxyethanol, Sensiva SC50, triethanolamine；
It is prepared by S2, oil phase mixed liquor：Each raw material of oil phase is mixed, heating stirring is uniform, obtains oil phase mixed liquor；
S3, water mix liquid preparation：First Acritamer 940 is swollen in deionized water, is configured to solution A, then by solution A
It is mixed with remaining Aqueous Phase Raw Material, heats mixing, obtain water and mix liquid；
S4, three mix：Water is mixed liquid to be added in oil phase mixed liquor, homogeneous, after stirring cooling, be added after adding
Each component raw material in phase, then homogeneous, vacuumize, be stirred for cooling to get.
Preferably, in the step S2,85 ± 2 DEG C are heated to；
In the step S3, the mass concentration of solution A is 1%, is heated to 60-70 DEG C；
The step S4 is specially：Water is mixed liquid to be slowly added into oil phase mixed liquor, 3000rpm homogeneous
20min, stirring defoaming is cooled to 45-50 DEG C, is kept stirring 30min, each component raw material after adding plus in phase,
2000rpm homogeneous 15min, vacuumize de-bubbled, stirring cooling to get.
The present invention also provides a kind of eye cream containing algal polysaccharides, in terms of mass fraction 100%, including following mass fraction
Each component：Foregoing algal polysaccharides 1-5%, aloe polysaccharide extracting solution 10-20%, camellia caul-fat 3-5%, shea butter 3-
5%, butanediol 5-10%, saualane 5-10%, beeswax 3-6%, cetostearyl alcohol and cetearyl glucoside
Montanov683-6%, Acritamer 940 0.3-0.5%, sodium hydroxide 0.1-0.5%, panthenol 0.5-2%, vitamin E acetic acid
Ester 0.5-2%, ceramide 0.1-0.2%, Phenoxyethanol 0.3-0.5%, Sensiva SC50 0.3-0.5%, hyaluronic acid
Sodium 0.1-0.3%, frankincense essential oil 0.3-1%, essence 0.2-0.5%, EDETATE SODIUM 0.05-0.1%, surplus is deionized water.
The present invention also provides a kind of preparation methods of the eye cream containing algal polysaccharides, comprise the following steps：
A1, each component raw material is weighed：
Oil phase：Camellia caul-fat, shea butter, saualane, beeswax, cetostearyl alcohol and cetearyl glucoside
Montanov68, ceramide；
Water phase：Algal polysaccharides, aloe polysaccharide extracting solution, butanediol, Acritamer 940, EDETATE SODIUM, deionized water；
Afterwards plus phase：Frankincense essential oil, Vitwas E, panthenol, sodium hyaluronate, essence, Phenoxyethanol, ethylhexyl
Glycerine, sodium hydroxide；
It is prepared by A2, oil phase mixed liquor：Each raw material of oil phase is mixed, heating stirring is uniform, obtains oil phase mixed liquor；
A3, water mix liquid preparation：First Acritamer 940 is swollen in deionized water, is configured to solution B, then by solution B
It is mixed with remaining Aqueous Phase Raw Material, heats mixing, obtain water and mix liquid；
A4, three mix：Water is mixed liquid to be slowly added into oil phase mixed liquor, homogeneous, stirring cooling, after adding
Add each component raw material in phase, then homogeneous, vacuumize, stirring cooling to get.Weigh each component raw material：
Preferably, in the step A2, it is heated to 80-85 DEG C；
In the step A3, the mass concentration of solution B is 1%, is heated to 70-75 DEG C；
The step A4 is specially：Water is mixed liquid to be slowly added into oil phase mixed liquor, 3000rpm homogeneous 20min,
Stirring defoaming, is cooled to 50-55 DEG C, is kept stirring 30min, each component raw material after adding plus in phase, 2000rpm homogeneous
15min, vacuumizes de-bubbled, stirring cooling to get.
Compared with prior art, the present invention has following advantageous effect：
The algal polysaccharides molecular weight of gained of the invention is relatively low, compared with high molecular weight algal polysaccharides, is retaining close moisturizing
Property while there is stronger inoxidizability, trophism and penetration, can accelerate the metabolism of cell, increase skin elasticity,
The aging of delaying skin.And with good water solubility, can in cosmetics with other raw material interworkings, before having a wide range of applications
Scape.
Specific embodiment
With reference to specific embodiment, the present invention is described in detail.Following embodiment will be helpful to the technology of this field
Personnel further understand the present invention, but the invention is not limited in any way.It should be pointed out that the ordinary skill to this field
For personnel, without departing from the inventive concept of the premise, various modifications and improvements can be made.These belong to the present invention
Protection domain.
Seaweed employed in the following embodiments of the present invention and comparative example is sargassum fusifome.The algal polysaccharides detection of preparation
In, the measure of the hydroscopicity and moisturizing rate is carried out according to following methods.
Hydroscopicity assay method：Precision weighs a certain amount of sample to be tested and (is denoted as W0), it is put into the culture dish of a diameter of 7cm
In, culture dish is positioned in drier, drier bottom replaces silica gel with saturated ammonium sulfate solution, and holding relative humidity is
81%, temperature is 25 DEG C, places the weight W of sample after a period of timen, hydroscopicity %=(Wn-W0)/W0*100。
Moisturizing rate assay method：Sample to be tested is made into the solution that mass fraction is 1%, precision pipettes 10mL in a diameter of
In the culture dish of 7cm, culture dish is positioned in drier, it is 81% to keep relative humidity, and temperature is 25 DEG C.It weighs and puts respectively
Put water content H after a period of timenWith water content H before placement0, moisturizing rate %=100*Hn/H0。
Embodiment 1
A kind of preparation method of algal polysaccharides is present embodiments provided, is comprised the following steps：
Step 1：Removal of impurities
It is (1 by solid-to-liquid ratio g/mL：20), the seaweed of dries pulverizing is extracted 4 hours with methanol eddy, filtering, filtrate
Recycle methanol；Filter residue volatilizes residual methanol, is fully dried at 45 DEG C, spare.
Step 2：Extraction
It by dried filter residue, is extracted with the hydrochloric acid solution of 0.2M, the solid-liquid ratio of filter residue and hydrochloric acid solution is 1:30,
Extracting temperature is 75 DEG C, 2 hours of extraction, and filtrate is steamed in the case where vacuum degree is 0.098Mpa near and done, obtains semifluid shape by filtering
Crude extract.
Step 3：Alcohol precipitation
Ethyl alcohol is slowly added in above-mentioned crude extract, and is stirred continuously, until concentration of alcohol is 50%, when standing 48 is small, mistake
Filter, filtrate recycling ethanol are spare.
Step 4：Dialysis
Sample solution obtained by step 3 is used into polysaccharide of the membrane filter method molecular cut off for 8-35kDa, is concentrated, freezing is dry
It is dry to get molecular weight be 8-35kDa algal polysaccharides.
In the research for extracting technique, the present embodiment 1 be using the inoxidizability of extract and moisture retention as guide, it is right
It raises each position and carries out inoxidizability and the measure of performance of keeping humidity respectively, as a result see Tables 1 and 2 respectively.
1 inoxidizability measurement result of table
2 moisture absorption of table and moisture retention measurement result
As can be known from the results of Table 1, present invention gained algal polysaccharides have more significant antioxidation activity.From the result of table 2
Understand, present invention gained algal polysaccharides with good antioxidation activity simultaneously, it may have significant moisturizing activity.
Embodiment 2
A kind of preparation method of algal polysaccharides is present embodiments provided, is comprised the following steps：
Step 1：Removal of impurities
It is (1 by solid-to-liquid ratio g/mL：30), the seaweed of dries pulverizing is extracted 2 hours with methanol eddy, filtering, filtrate
Recycle methanol；Filter residue volatilizes residual methanol, is fully dried at 45 DEG C, spare.
Step 2：Extraction
It by dried filter residue, is extracted with the hydrochloric acid solution of 0.2M, the solid-liquid ratio of filter residue and hydrochloric acid solution is 1：15,
Extracting temperature is 70 DEG C, 4 hours of extraction, and filtrate is steamed in the case where vacuum degree is 0.085Mpa near and done, obtains semifluid shape by filtering
Crude extract.
Step 3：Alcohol precipitation
Ethyl alcohol is slowly added in above-mentioned crude extract, and is stirred continuously, until concentration of alcohol is 30%, when standing 36 is small, mistake
Filter, filtrate recycling ethanol are spare.
Step 4：Dialysis
Sample solution obtained by step 3 is used into polysaccharide of the membrane filter method molecular cut off for 8-35kDa, is concentrated, freezing is dry
It is dry to get molecular weight be 8-35kDa algal polysaccharides.
Embodiment 3
A kind of preparation method of algal polysaccharides is present embodiments provided, is comprised the following steps：
Step 1：Removal of impurities
It is (1 by solid-to-liquid ratio g/mL：25), the seaweed of dries pulverizing is extracted 3 hours with methanol eddy, filtering, filtrate
Recycle methanol；Filter residue volatilizes residual methanol, is fully dried at 45 DEG C, spare.
Step 2：Extraction
It by dried filter residue, is extracted with the hydrochloric acid solution of 0.1M, the solid-liquid ratio of filter residue and hydrochloric acid solution is 1：20,
Extracting temperature is 70 DEG C, 4 hours of extraction, and filtrate is steamed in the case where vacuum degree is 0.085Mpa near and done, obtains semifluid shape by filtering
Crude extract.
Step 3：Alcohol precipitation
Ethyl alcohol is slowly added in above-mentioned crude extract, and is stirred continuously, until concentration of alcohol is 40%, when standing 24 is small, mistake
Filter, filtrate recycling ethanol are spare.
Step 4：Dialysis
Sample solution obtained by step 3 is used into polysaccharide of the membrane filter method molecular cut off for 8-35kDa, is concentrated, freezing is dry
It is dry to get molecular weight be 8-35kDa algal polysaccharides.
Comparative example 1
This comparative example is identical with the preparation method of embodiment 1, the difference is that only：In step 2, this comparative example uses
Extraction with aqueous solution.
Comparative example 2
This comparative example is identical with the preparation method of embodiment 1, is only that before different：In step 2, this comparative example uses
Extracting temperature be 80 DEG C.
Comparative example 3
This comparative example is identical with the preparation method of embodiment 1, is only that before different：In step 2, this comparative example uses
Filter residue and hydrochloric acid solution solid-liquid ratio be 1:10.
In embodiment 2 and 3, the extraction process of comparative example 1-3, raise each position and carry out inoxidizability and moisture retention respectively
The measure of energy, the results are shown in Table 3 and table 4.
3 embodiment 2 and 3 of table, the inoxidizability measurement result of comparative example 1-3
The moisture absorption of algal polysaccharides and moisture retention measurement result that 4 embodiment 2 and 3 of table, comparative example 1-3 are obtained
It was found from the result of table 1 and table 3, compared with comparative example, gained algal polysaccharides have better the embodiment of the present invention
Antioxidation activity.The result of table 2 and table 4 understands that the embodiment of the present invention is possessing the same of good oxidation resistance compared with comparative example
When have better performance of keeping humidity.
Skin lotion of the embodiment 4 containing algal polysaccharides
A kind of skin lotion containing algal polysaccharides is present embodiments provided, its preparation method is as follows：
Oil phase：Silicone grease (9040), caprylic/capric triglyceride, jojoba oil, octadecyl alcolol, Brij72 polyoxyethylene stearyls
Base ether 2, Brij721 polyoxyethylene stearyl base ethers.
Water phase：The algal polysaccharides of molecular weight 8-35kDa, aloe polysaccharide extracting solution, butanediol, Acritamer 940, EDTA bis-
Sodium, deionized water.
Afterwards plus phase：Essence, Phenoxyethanol, Sensiva SC50, triethanolamine.
The parts by weight of above-mentioned each raw material components are as follows：
The algal polysaccharides 1-3% of molecular weight 8-35kDa, aloe polysaccharide extracting solution 10-20%, silicone grease (9040) 5-10%,
Caprylic/capric triglyceride 3-6%, jojoba oil 3-6%, octadecyl alcolol 2-5%, Brij72 polyoxyethylene stearyl base ether 2-4%,
Brij721 polyoxyethylene stearyl base ether 2-4%, butanediol 3-5%, Acritamer 940 0.2-0.5%, Phenoxyethanol 0.5-
0.8%, Sensiva SC50 0.5-0.8%, EDETATE SODIUM 0.05-0.1%, triethanolamine 0.2-0.4%, essence 0.3-
0.8%, surplus is deionized water.
Step：Each raw material of oil phase is added in into main emulsification pot heating stirring to 85 ± 2 DEG C, treats fully to dissolve mixing；Water is mutually former
First Acritamer 940 is fully swollen in deionized water in material, is configured to 1% solution, then it is auxiliary with the addition of remaining Aqueous Phase Raw Material
Pot is emulsified, mixing is heated to 60-70 DEG C.Water is mutually slowly added to oil phase, 3000rpm homogeneous 20min, stirring defoaming is cooled to
45-50 DEG C, be kept stirring 30min, after addition plus phase, 2000rpm homogeneous 15min vacuumize de-bubbled, stirring cooling to get.
The skin cream that the present invention prepares for skin surfaces such as hand, face, necks, has and moistens moisturizing, mitigates wrinkle
The effect of line, anti-aging.
Eye cream of the embodiment 5 containing algal polysaccharides
A kind of eye cream containing algal polysaccharides is present embodiments provided, its preparation method is as follows：
A1, each component raw material is weighed：
Oil phase：Camellia caul-fat, shea butter, saualane, beeswax, cetostearyl alcohol and cetearyl glucoside
Montanov68, ceramide；
Water phase：Algal polysaccharides, aloe polysaccharide extracting solution, butanediol, Acritamer 940, EDETATE SODIUM, deionized water；
Afterwards plus phase：Frankincense essential oil, Vitwas E, panthenol, sodium hyaluronate, essence, Phenoxyethanol, ethylhexyl
Glycerine, sodium hydroxide；
The parts by weight of above-mentioned each raw material components are as follows：
The algal polysaccharides 1-5% of molecular weight 8-35kDa, aloe polysaccharide extracting solution 10-20%, camellia caul-fat 3-5%, breast wood
Fruit oil 3-5%, butanediol 5-10%, saualane 5-10%, beeswax 3-6%, cetostearyl alcohol (and) cetearyl glucoside
Montanov68 3-6%, Acritamer 940 0.3-0.5%, sodium hydroxide 0.1-0.5%, panthenol 0.5-2%, vitamin E vinegar
Acid esters 0.5-2%, ceramide 0.1-0.2%, Phenoxyethanol 0.3-0.5%, Sensiva SC50 0.3-0.5%, hyalomitome
Sour sodium 0.1-0.3%, frankincense essential oil 0.3-1%, essence 0.2-0.5%, EDETATE SODIUM 0.05-0.1%, surplus is deionization
Water.
Step：Each raw material of oil phase is added in into main emulsification pot heating stirring to 80-85 DEG C, treats fully to dissolve mixing；Water is mutually former
First Acritamer 940 is fully swollen in deionized water in material, is configured to 1% solution, then it is auxiliary with the addition of remaining Aqueous Phase Raw Material
Pot is emulsified, mixing is heated to 70-75 DEG C.Water is mutually slowly added to oil phase, 3000rpm homogeneous 20min, stirring defoaming is cooled to
50-55 DEG C, be kept stirring 30min, after addition plus phase, 2000rpm homogeneous 15min vacuumize de-bubbled, stirring cooling to get.
The eye cream that the present invention prepares for circumference of eyes, has effects that moist moisturizing, mitigates wrinkle, slows down aging.
In addition, with the effect of embodiment in patent CN101250232A comparatively, the seaweed for preparing of the method for the present invention is more
Sugared inoxidizability is stronger, and invention also provides the performance of keeping humidity investigation data of extract, shows that moistening effect is notable.
In conclusion the present invention prepare gained molecular weight be 8-35kDa algal polysaccharides, have notable antioxidation and
Moisturizing activity, suitable for preparing moisturizing class and anti-aging class cosmetics.And with good water solubility, can in cosmetics with its
His raw material interworking, is with a wide range of applications.
The above description of the embodiments is intended to facilitate ordinary skill in the art to understand and use the invention.
Person skilled in the art obviously can easily make these embodiments various modifications, and described herein general
Principle is applied in other embodiment without having to go through creative labor.Therefore, the invention is not restricted to above-described embodiment, abilities
Field technique personnel announcement according to the present invention, improvement and modification made without departing from the scope of the present invention all should be the present invention's
Within protection domain.
Claims (8)
1. a kind of preparation method of algal polysaccharides, which is characterized in that the preparation method includes the following steps：After seaweed is crushed
It cleans through methanol, after the abundant methanol removal of residue hot acid extraction method is recycled to extract wherein acid-soluble polysaccharide, then removed by alcohol precipitation
It after the macromolecular polysaccharide constituents that bioavailability is relatively low, surplus solution is gone to recycle ethyl alcohol, dialyses, freeze-drying obtains molecule
Measure the algal polysaccharides of 8-35kDa；
The preparation method is as follows：
Removal of impurities：By the seaweed of dries pulverizing with methanol eddy extraction 2-4 it is small when, filtering, filtrate recycling methanol；Filter residue volatilizes remnants
Methanol, it is fully dry at 45-50 DEG C, it is spare；
Extraction：Dried filter residue is extracted with hydrochloric acid solution, Extracting temperature is 70-75 DEG C, when extraction 2-4 is small, filtering,
Filtrate is evaporated under vacuum, obtains crude extract；
Alcohol precipitation：The crude extract obtained by extraction step is soluble in water, ethyl alcohol is added, after stirring evenly, it is small to stand 24-48
When, filtering is spare after filtrate removes ethyl alcohol；
Dialysis：Gained filtrate after alcohol precipitation is used into algal polysaccharides of the membrane filter method molecular cut off for 8-35kDa, is concentrated, freezing
It is dry to get；
In the extraction step, the molar concentration of hydrochloric acid solution is 0.1-0.2M；The solid-liquid ratio of filter residue and hydrochloric acid solution is, per 1g
Filter residue corresponds to the hydrochloric acid solution of 15-30mL；Vacuum condition is vacuum degree 0.085-0.098Mpa.
2. the preparation method of algal polysaccharides according to claim 1, which is characterized in that in the removal step：Seaweed with
The amount ratio of methanol is that the methanol of 20-30mL is corresponded to per 1g seaweed.
3. the preparation method of algal polysaccharides according to claim 1, which is characterized in that in the alcohol precipitation step, addition
The volumetric concentration of ethyl alcohol in water is 30-50%.
4. the algal polysaccharides that a kind of preparation method according to any one of claims 1 to 3 algal polysaccharides obtains are in cosmetics
In purposes.
5. a kind of skin lotion containing algal polysaccharides, which is characterized in that in terms of mass fraction 100%, including following mass fraction
Each component：Algal polysaccharides 1-3% prepared by any one of claims 1 to 3 the method, aloe polysaccharide extracting solution 10-20%, silicon
Fat 5-10%, caprylic/capric triglyceride 3-6%, jojoba oil 3-6%, octadecyl alcolol 2-5%, Brij72 polyoxyethylene stearyl
Base ether 2-4%, Brij721 polyoxyethylene stearyl base ether 2-4%, butanediol 3-5%,
Acritamer 940 0.2-0.5%, Phenoxyethanol 0.5-0.8%, Sensiva SC50 0.5-0.8%, EDETATE SODIUM 0.05-
0.1%, triethanolamine 0.2-0.4%, essence 0.3-0.8%, surplus are deionized water.
6. a kind of preparation method of the skin lotion according to claim 5 containing algal polysaccharides, which is characterized in that including following
Step：
S1, each component raw material is weighed：
Oil phase：Silicone grease, caprylic/capric triglyceride, jojoba oil, octadecyl alcolol, Brij72 polyoxyethylene stearyl base ethers 2,
Brij721 polyoxyethylene stearyl base ethers；
Water phase：Algal polysaccharides, aloe polysaccharide extracting solution, butanediol, Acritamer 940, EDETATE SODIUM, deionized water；
Afterwards plus phase：Essence, Phenoxyethanol, Sensiva SC50, triethanolamine；
It is prepared by S2, oil phase mixed liquor：Each raw material of oil phase is mixed, heating stirring is uniform, obtains oil phase mixed liquor；
S3, water mix liquid preparation：First Acritamer 940 is swollen in deionized water, is configured to solution A, then by solution A and its
Remaining Aqueous Phase Raw Material mixing, heats mixing, obtains water and mix liquid；
S4, three mix：Water is mixed liquid to be added in oil phase mixed liquor, homogeneous, after stirring cooling, after adding plus in phase
Each component raw material, then homogeneous vacuumizes, stirring cooling to get.
7. a kind of eye cream containing algal polysaccharides, which is characterized in that in terms of mass fraction 100%, including each of following mass fraction
Component：Algal polysaccharides 1-5% prepared by any one of claims 1 to 3 the method, aloe polysaccharide extracting solution 10-20%, camellia
Caul-fat 3-5%, shea butter 3-5%, butanediol 5-10%, saualane 5-10%, beeswax 3-6%, cetostearyl alcohol and spermaceti
Stearyl glucoside Montanov68 3-6%, Acritamer 940 0.3-0.5%, sodium hydroxide 0.1-0.5%, panthenol 0.5-
2%, Vitwas E 0.5-2%, ceramide 0.1-0.2%, Phenoxyethanol 0.3-0.5%, Sensiva SC50 0.3-
0.5%, Sodium Hyaluronate 0.1-0.3%, frankincense essential oil 0.3-1%, essence 0.2-0.5%, EDETATE SODIUM 0.05-0.1% are remaining
It measures as deionized water.
8. a kind of preparation method of the eye cream according to claim 7 containing algal polysaccharides, which is characterized in that including following step
Suddenly：
A1, each component raw material is weighed：
Oil phase：Camellia caul-fat, shea butter, saualane, beeswax, cetostearyl alcohol and cetearyl glucoside
Montanov68, ceramide；
Water phase：Algal polysaccharides, aloe polysaccharide extracting solution, butanediol, Acritamer 940, EDETATE SODIUM, deionized water；
Afterwards plus phase：Frankincense essential oil, Vitwas E, panthenol, sodium hyaluronate, essence, Phenoxyethanol, Sensiva SC50,
Sodium hydroxide；
It is prepared by A2, oil phase mixed liquor：Each raw material of oil phase is mixed, heating stirring is uniform, obtains oil phase mixed liquor；
A3, water mix liquid preparation：First Acritamer 940 is swollen in deionized water, is configured to solution B, then by solution B and its
Remaining Aqueous Phase Raw Material mixing, heats mixing, obtains water and mix liquid；
A4, three mix：Water is mixed liquid to be slowly added into oil phase mixed liquor, homogeneous, stirring cooling adds phase after adding
In each component raw material, then homogeneous vacuumizes, stirring cooling to get.
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| CN107223760A (en) * | 2017-08-06 | 2017-10-03 | 长沙小新新能源科技有限公司 | A kind of Enteromorpha ice cream and preparation method thereof |
| CN107334686A (en) * | 2017-08-18 | 2017-11-10 | 广州丸美生物科技有限公司 | Skin matrix, preparation method and applications and cosmetics and preparation method thereof |
| CN107874261A (en) * | 2017-12-14 | 2018-04-06 | 荣成市飞创科技有限公司 | The preparation method of one Polysaccharides From Laminaria Japonica health food |
| CN111067833A (en) * | 2020-01-09 | 2020-04-28 | 广州市美驰化妆品有限公司 | Linseed meal extract and preparation method and application thereof |
| CN113952252A (en) * | 2021-12-01 | 2022-01-21 | 雅诗蓝生物护肤科技(深圳)有限公司 | Preparation method of cosmetic eye cream compounded by functional polysaccharide |
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