CN113413329A - Dendrobium nobile polysaccharide herbal face cream and preparation method thereof - Google Patents
Dendrobium nobile polysaccharide herbal face cream and preparation method thereof Download PDFInfo
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Abstract
The application discloses dendrobium nobile polysaccharide herbal face cream in the technical field of traditional Chinese medicine application, and the raw materials comprise, by mass, 2% -10% of white vaseline, 1% -2% of glyceryl monostearate, 1% -2% of stearyl cetyl alcohol, 1% -2% of cetyl alcohol, 3% -10% of shea butter, 0.05% -1% of dendrobium nobile polysaccharide, 60% -80% of ultrapure water, 10% -20% of propylene glycol and 5% -10% of polyglycerol-10 stearate. The prepared dendrobium nobile polysaccharide herbal cream has the effects of resisting oxidation and promoting fibroblast growth (resisting wrinkles), and is externally applied for a long time to protect the skin and help to remove harmful substances from the skin.
Description
Technical Field
The invention relates to the technical field of application of traditional Chinese medicinal materials, and in particular relates to dendrobium stem polysaccharide herbal face cream and a preparation method thereof.
Background
With the development of the cosmetic industry and the pursuit of people for beauty, the quality and safety of cosmetics are emphasized more while the effect of skin care products on skin is emphasized, more and more people favor the cosmetics based on natural products, and in order to effectively delay skin aging, the counter measures which can be taken in the field of cosmetics are as follows: supplying water and oil to maintain water-oil balance, inhibiting excessive free radical production (antioxidation), supplying skin collagen and elastin, inhibiting tyrosinase activity and melanin production, inhibiting non-enzymatic glycosylation reaction, etc. The oxidation resistance is an important consideration factor of the quality of cosmetics, but at present, some products based on natural products on the market only add certain components in a targeted manner, and cannot play an oxidation resistance role while effectively resisting wrinkles.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the face cream which is prepared from natural products, has the functions of effectively resisting wrinkles and resisting oxidation.
One of the purposes of the invention is to provide application of dendrobium nobile lindl polysaccharide in cosmetics or skin care products with anti-wrinkle effect.
The invention also aims to provide application of the dendrobium nobile lindl polysaccharide in cosmetics or skin care products with anti-wrinkle and anti-oxidation effects.
The invention aims to provide dendrobium nobile polysaccharide herbal face cream, which comprises 2-10% of white vaseline, 1-2% of glyceryl monostearate, 1-2% of stearyl cetyl alcohol, 1-2% of cetyl alcohol, 3-10% of shea butter, 0.05-1% of dendrobium nobile polysaccharide, 60-80% of ultrapure water, 10-20% of propylene glycol and 5-10% of polyglycerol-10 stearate by mass.
The working principle and the beneficial effects of the invention are as follows: dendrobium nobile is a commonly used rare Chinese medicinal material belonging to the genus Dendrobium of the family Orchidaceae, which contains effective components such as alkaloid, polysaccharide, flavonoid and phenols, and substances extracted from the dendrobium nobile are widely used in medicines, foods and cosmetics.
In the invention, the white vaseline has the main functions of: a lubricant, an ointment base; the glyceryl monostearate has oleophilic and hydrophilic groups, and has the functions of moistening, emulsifying and the like; the stearic acid: octadecanoic acid is widely applied to preparation of vanishing cream and cold cream in cosmetics to achieve the emulsification effect. Generally, alkali is used for neutralization when the stearic acid is added, but the addition of an alkali agent is not generally considered, so that the absorption of acid by the skin can be adjusted by adding a small amount of stearic acid; the stearic cetyl alcohol: white solid crystals, particles or wax blocks, and has the effects of inhibiting greasy feeling, reducing viscosity of wax raw materials, stabilizing emulsion of cosmetics, and the like; in various cosmetics, as a base; the effect is better when the composition is matched with hexadecanol; the cetyl alcohol: the emulsion is suitable for cream and emulsification, is used as a matrix, an emulsifier cream, an ointment matrix and the like, has the effects of inhibiting greasy feeling and reducing viscosity of wax raw materials, has similar effect with stearyl cetyl alcohol, and has better matching use effect; the shea butter comprises the following components: the skin care product is rich in unsaturated fatty acid, is easy to absorb, can enhance the oxidation resistance of the skin, is solid oil, can promote cell regeneration, and has a skin nutrition function; the polyglycerol-10 stearate: the high polymer emulsifier has good hydrophilicity, can form o/w type emulsified liquid, and has the functions of stabilizing and thickening; the used water is ultrapure water subjected to impurity removal, so that the influence of impurities on the face cream production is avoided; the propylene glycol is used as a safe antioxidant, has an antioxidant effect on the face cream and has an antioxidant water-locking capacity on the skin.
Compared with the prior art, the invention has the beneficial effects that:
the dendrobium nobile polysaccharide herbal cream prepared by extracting dendrobium nobile lindl polysaccharide beneficial to human skin from the traditional Chinese herbal medicine dendrobium nobile lindl has the effects of resisting oxidation and promoting the growth of fibroblasts (resisting wrinkles), and is externally applied for a long time to protect the skin and help to remove harmful substances of the skin.
Simultaneously through multiple proportion combination contrast, the cream of this application has better feel, more affine and laminating facial skin, can be better guarantee the effect of dendrobium nobile polysaccharide.
Specifically, the dendrobium nobile lindl polysaccharide is extracted by adopting the following method: taking dried stems of dendrobium nobile, boiling in water for repeatedly extracting for three times, combining filtrates, concentrating, adding absolute ethanol for precipitating for 12h, taking a precipitate part for centrifugation at 4000r/min, adding water to the precipitate for dissolving, and volatilizing ethanol to obtain dendrobium nobile total sugar for later use; pretreating macroporous adsorbent resin with 95% ethanol for 24h, washing with distilled water, soaking concentrated herba Dendrobii total sugar in macroporous adsorbent resin for 24h, washing with distilled water for three times, washing with 10% ethanol for one time, mixing filtrates, concentrating, and freeze drying in freeze dryer to obtain herba Dendrobii polysaccharide.
The fourth purpose of the invention is to provide a preparation method of dendrobium nobile polysaccharide herbal cream, which comprises the following specific steps:
step one, dissolving prepared materials: preparing materials according to a certain proportion, dissolving dendrobium nobile polysaccharide in ultrapure water, fully dissolving, centrifuging by using a centrifuge, and taking supernatant as a water phase solution;
step two, water phase emulsification: adding a dendrobium nobile polysaccharide solution into a suitable container, adding propylene glycol and polyglycerol-10 stearate, putting into a constant-temperature magnetic stirrer, stirring at a constant speed of 75-85 ℃, and fully emulsifying to obtain a water phase;
step three, emulsifying an oil phase: respectively adding white vaseline, glyceryl monostearate, 1-2% stearyl cetyl alcohol, cetyl alcohol and shea butter into a suitable container, putting into a constant-temperature magnetic stirrer, stirring at a constant speed of 75-85 ℃, and fully emulsifying to obtain an oil phase;
step four, mixing water and oil: slowly adding a small amount of the water phase into the oil phase, and slowly stirring until the water phase and the oil phase are fully mixed;
step five, heat preservation and cooling: and (3) after the mixture of the water phase and the oil phase is stirred, preserving the heat for 2-3 minutes, taking out, and cooling to room temperature to obtain a finished product.
Further, dissolving the dendrobium nobile polysaccharide in ultrapure water for 24h, centrifuging for 10min by using a 10000r/min centrifuge, and taking the supernatant as an aqueous phase solution after centrifugation.
Further, the temperature of the constant-temperature magnetic stirrer in the second step and the third step is 85 ℃.
Drawings
FIG. 1 is a comparison of the appearance of the cream of example 1 and the cream without dendrobium stem polysaccharide;
FIG. 2 is a graph comparing the protective effect of Dendrobium nobile polysaccharide on fibroblast 3T3L 1;
FIG. 3 is a graph showing the antioxidant effect of Dendrobium nobile Lindl polysaccharide in example 3.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1: a preparation method of dendrobium nobile polysaccharide anti-wrinkle and anti-oxidation face cream specifically comprises the following steps:
(1) extracting dendrobium nobile polysaccharide: taking 1000g of dendrobium stem, boiling 20L of dendrobium stem in water for 3h, repeatedly extracting for three times, combining filtrates, concentrating to 500mL, adding 2L of absolute ethyl alcohol for precipitation for 12h, taking 4000r/min of precipitate for centrifugation for 20min, adding 500mL of precipitate for dissolving in water, volatilizing the ethanol for later use, taking 500g of D101 macroporous adsorption resin, pretreating with 95% ethanol for 24h, cleaning the ethanol with distilled water, pouring the concentrated dendrobium stem total sugar into the D101 macroporous adsorption resin for soaking for 24h, washing with distilled water for three times, washing with 10% ethanol for one time, combining filtrates, concentrating, and freeze-drying by a freeze dryer to obtain the dendrobium stem polysaccharide.
(2) Emulsification of water phase: dissolving 14mg of dendrobium nobile polysaccharide in 12.59mL of ultrapure water, wherein the proportion of the dendrobium nobile polysaccharide is 0.08 percent and the proportion of the ultrapure water is 69.9 percent, adding 2.7g of propylene glycol serving as an antioxidant, adding 1.08g of polyglycerol-10 stearate serving as an emulsifier, and putting the mixture into a constant-temperature magnetic stirrer to stir at a constant speed of about 85 ℃ for full emulsification.
(3) Oil phase emulsification: adding white vaseline 0.36g, glyceryl monostearate 0.36g, stearyl cetyl alcohol 0.18g, cetyl alcohol 0.18g and shea butter 0.54g into a container, and stirring at 85 deg.C with a constant temperature magnetic stirrer to emulsify completely.
(4) And after the oil phase is stirred, slowly adding the water phase into the oil phase, slowly stirring after adding, stirring until the water phase is completely poured into the oil phase, and continuously stirring for 5-6 minutes until the water phase and the oil phase are fully mixed.
(5) And (3) after the water phase and oil phase mixture is stirred, preserving the heat for 2-3 minutes, taking out, and cooling to room temperature at room temperature to obtain a finished product.
The following is a comparison of the texture of the creams prepared under the preparation method of example 1 in different proportions.
TABLE 1
The comparison shows that the cream of the invention has good texture, proper oiliness, proper consistency, proper coating, uniform whole, no particles and no mud, while the cream of the invention of the examples 1 to 5 has poor texture, especially after stearic acid is added, although stearic acid is a common raw material in the field, the texture and the absorption effect of the whole cream are affected, and even people can disintegrate and can not obtain the cream, thus proving that the formula of the cream of the invention is most suitable.
In addition, as shown in fig. 1, as can be seen from the comparison of the appearance of the cream in example 1 with that of the cream without dendrobium nobile polysaccharide, the cream of the present invention is uniform and has a more suitable consistency.
MTT method for detecting proliferation effect of dendrobium nobile polysaccharide on 3T3L1 fibroblast
Cell culture: 3T3L1 cells were cultured in DMEM high-glucose medium containing 10% newborn bovine serum (NBCS) at 37 ℃ with 5% CO2Culturing in a cell culture box.
Cell plating: taking cells in logarithmic growth phase at 1.5X 103Inoculating to 96-well plate at 37 deg.C and 5% CO2Was incubated in the cell incubator of (1) for 12 h.
Cell dosing: after the cells grow in an adherent manner, a control group and an additive group are arranged, a DMEM culture medium is used as the control group, the dendrobium nobile polysaccharide is prepared by a DMEM high-sugar culture medium to have final concentrations of 0.1mg/mL, 0.3mg/mL and 1mg/mL respectively as the additive group, and the dendrobium nobile polysaccharide is put into a cell culture box for culture for 48 hours.
MTT assay to detect 3T3L1 cell viability: and adding 20 mu L of 5mg/mL MTT solution into all the wells respectively, placing the wells in an incubator for incubation for 4h, removing the culture solution by suction, adding 150 mu L of DMSO into each well, keeping out of the light for 20min to fully dissolve crystals, and measuring the light absorption value at 490nm by using an enzyme-linked immunosorbent assay.
The fibroblast is an important cell on the surface of the skin, and has an important effect in the aspect of aging, and as shown in figure 2, the dendrobium nobile polysaccharide has a good effect of promoting the growth of the fibroblast, can be used as a main active ingredient of dendrobium nobile face cream, and can improve the anti-aging activity of the face cream.
Determination of ability of dendrobium nobile polysaccharide to eliminate DPPH free radical
Preparation of DPPH free radical solution: the DPPH was dissolved in 70% methanol to prepare a 0.2mmol/L DPPH solution.
Preparing a dendrobium nobile polysaccharide test solution: precisely weighing 96mg of dendrobium nobile polysaccharide, adding 30ml of ultrapure water to dissolve overnight, centrifuging at 1100rpm for 10min, and taking supernatant to obtain 3.2mg/ml sample mother liquor. Setting the concentration gradient of dendrobium nobile polysaccharide drug as 1.6mg/ml, 0.8mg/ml, 0.4mg/ml, 0mg/ml, 4 concentration gradients. 3 sets of replicates were run at each concentration.
Absorbance of test solution and control solution: 2ml of sample solutions with different concentrations were taken, 2ml of 0.2mmol/L DPPH solution was added, mixed well, reacted in a dark room for 30min, and the absorbance (AX1) was measured at 517nm using a spectrophotometer. Meanwhile, 2ml of sample solutions with different concentrations were taken, 2ml of 70% methanol was added, mixed well, reacted in a dark room for 30min, and then the absorbance (AX2) was measured at 517 mm. 2ml of 70% methanol was added to 2ml of 0.2mmol/L DPPH solution, mixed well, and reacted in the dark for 30min as a blank (A0).
The DPPH radical clearance rate of polysaccharides can be expressed as:
DPPH clearance (100%) (1-AX/A0) 100%
In the formula: AX is the absorbance of the sample after reaction with DPPH solution, and A0 is the absorbance of 70% methanol after reaction with DPPH solution.
The ability of dendrobium nobile polysaccharides to scavenge DPPH free radicals is plotted with the experimental concentration of a sample as the abscissa and the corresponding free radical scavenging rate as the ordinate. The results are shown in fig. 3, and the dendrobium nobile polysaccharide has good activity of eliminating DPPH free radicals.
The foregoing is merely an example of the present invention and common general knowledge of known specific structures and features of the embodiments is not described herein in any greater detail. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several changes and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (7)
1. Application of herba Dendrobii polysaccharide in cosmetic or skin care product with anti-wrinkle effect is provided.
2. Application of herba Dendrobii polysaccharide in cosmetics or skin care products with anti-wrinkle and antioxidant effects is provided.
3. The dendrobium nobile polysaccharide herbal face cream is characterized in that: the raw materials comprise, by mass, 2% -10% of white vaseline, 1% -2% of glyceryl monostearate, 1% -2% of stearyl cetyl alcohol, 1% -2% of cetyl alcohol, 3% -10% of shea butter, 0.05% -1% of dendrobium nobile polysaccharide, 60% -80% of ultrapure water, 10% -20% of propylene glycol and 5% -10% of polyglycerol-10 stearate.
4. The dendrobium nobile polysaccharide herbal facial cream of claim 3, characterized in that: the dendrobium nobile lindl polysaccharide is extracted by adopting the following method: taking dried stems of dendrobium nobile, boiling in water for repeatedly extracting for three times, combining filtrates, concentrating, adding absolute ethanol for precipitating for 12h, taking a precipitate part for centrifugation at 4000r/min, adding water to the precipitate for dissolving, and volatilizing ethanol to obtain dendrobium nobile total sugar for later use; pretreating macroporous adsorbent resin with 95% ethanol for 24h, washing with distilled water, soaking concentrated herba Dendrobii total sugar in macroporous adsorbent resin for 24h, washing with distilled water for three times, washing with 10% ethanol for one time, mixing filtrates, concentrating, and freeze drying in freeze dryer to obtain herba Dendrobii polysaccharide.
5. The preparation method of dendrobium nobile polysaccharide herbal cream according to claim 3 or 4, characterized in that: the method comprises the following specific steps:
step one, dissolving prepared materials: preparing materials according to a certain proportion, dissolving dendrobium nobile polysaccharide in ultrapure water, fully dissolving, centrifuging by using a centrifuge, and taking supernatant as a water phase solution;
step two, water phase emulsification: adding a dendrobium nobile polysaccharide solution into a suitable container, adding propylene glycol and polyglycerol-10 stearate, putting into a constant-temperature magnetic stirrer, stirring at a constant speed of 75-85 ℃, and fully emulsifying to obtain a water phase;
step three, emulsifying an oil phase: respectively adding white vaseline, glyceryl monostearate, 1-2% stearyl cetyl alcohol, cetyl alcohol and shea butter into a suitable container, putting into a constant-temperature magnetic stirrer, stirring at a constant speed of 75-85 ℃, and fully emulsifying to obtain an oil phase;
step four, mixing water and oil: slowly adding a small amount of the water phase into the oil phase, and slowly stirring until the water phase and the oil phase are fully mixed;
step five, heat preservation and cooling: and (3) after the mixture of the water phase and the oil phase is stirred, preserving the heat for 2-3 minutes, taking out, and cooling to room temperature to obtain a finished product.
6. The preparation method of dendrobium nobile polysaccharide herbal cream according to claim 5, characterized in that: dissolving dendrobium nobile lindl polysaccharide in ultrapure water for 24h, centrifuging for 10min by using a 10000r/min centrifugal machine, and taking supernatant as an aqueous phase solution after centrifugation.
7. The preparation method of dendrobium nobile polysaccharide herbal cream according to claim 6, characterized in that: the temperature of the constant-temperature magnetic stirrer in the second step and the third step is 85 ℃.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114533631A (en) * | 2022-03-18 | 2022-05-27 | 广西农业职业技术大学 | Kudzu vine root and dendrobium whitening cream and preparation method thereof |
| CN114732774A (en) * | 2022-03-11 | 2022-07-12 | 广东丸美生物技术股份有限公司 | Composition containing traditional Chinese medicine extract and preparation method and application thereof |
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