CN106236676A - A kind of vegetable composition with senile-resistant efficacy and application thereof - Google Patents
A kind of vegetable composition with senile-resistant efficacy and application thereof Download PDFInfo
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A61Q19/08—Anti-ageing preparations
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
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- A61K2236/30—Extraction of the material
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- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
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Abstract
The invention discloses a kind of vegetable composition with senile-resistant efficacy and application thereof.This vegetable composition is made up of the component of following percentage by weight: Radix Polygoni Multiflori 10 50%, Fructus Lycii 5 35%, Dendrobium nobile 5 35%, Radix Glycyrrhizae 5 30%.Vegetable composition uses water, ethanol, ethanol water to be Extraction solvent, and after the extraction of high pressure homogenization method machine and membrance concentration, concentrated solution crosses macroporous resin column, with ethanol solution repeatedly eluting, eluent is after membrance concentration, with 1,3 butanediols dissolve, and filter, i.e. can get vegetable composition.The vegetable composition of the present invention is applied in cosmetics, has and good promotes dermal cell growth, removes free radical, improves skin microcirculation, skin-whitening and effect of raising skin elasticity, thus reaches skin anti-aging purpose.
Description
Technical field
The present invention relates to a kind of vegetable composition and application thereof, particularly relate to a kind of defying age vegetable composition and changing
Application in cosmetic, belongs to cosmetic field.
Background technology
Aging is the direct performance that function of human body slows down, and is the mistake that gradually fails of the function of body parts tract
Journey, is also a kind of naturally process.The reason causing aging is extremely complex, sums up and mainly has endogenous cause of ill and two sides of exopathogenic factor
Face.Endogenous cause of ill is main and hereditary, endocrine is relevant, and the human free radical that exopathogenic factor mainly environment causes raises, and breaks free radical and produces
Balance between life and extinction, causes disease to produce and old and feeble.
Skin aging is that a kind of concrete of human senility embodies, and is embodied in and wrinkle, speckle occurs, the feature such as be dried.
Skin aging to be prevented should from promote cell growth, remove free radical, improve skin microcirculation etc. in terms of set about.
Prevention and delay skin aging are the important topics of cosmetic dermatology.Development effectiveness is obvious, safe and stable, suction
The activity of fighting against senium thing kept well, it has also become the focus of current beauty industry.Natural materials is applied to cosmetics and can reach skin and control
Treat and skin protection double effects, and there is diverse in function, the characteristic such as widely applicable, safe, so natural plants antisenescence work
Property thing is defying age main direction of studying.
Summary of the invention
It is an object of the invention to provide a kind of vegetable composition with senile-resistant efficacy and this vegetable composition
Preparation method.
To achieve the object of the present invention, present invention employs following technical scheme:
A kind of vegetable composition with senile-resistant efficacy, it is characterised in that be made up of the component of following percentage by weight: He Shou
Crow 10-50%, Fructus Lycii 5-35%, Dendrobium nobile 5-35%, Radix Glycyrrhizae 5-30%.
Preferably, the vegetable composition percentage by weight described in senile-resistant efficacy is: Radix Polygoni Multiflori 40%, Fructus Lycii 25%,
Dendrobium nobile 20%, Radix Glycyrrhizae 15%.
Preferably, vegetable composition (II) the amount percentage ratio described in senile-resistant efficacy is: Radix Polygoni Multiflori 38%, Fructus Lycii
22%, Dendrobium nobile 20%, Radix Glycyrrhizae 20%.
Preferably, the vegetable composition percentage by weight described in senile-resistant efficacy is: Radix Polygoni Multiflori 35%, Fructus Lycii 25%,
Dendrobium nobile 20%, Radix Glycyrrhizae 20%.
Preferably, the vegetable composition percentage by weight described in senile-resistant efficacy is: Radix Polygoni Multiflori 35%, Fructus Lycii 20%,
Dendrobium nobile 25%, Radix Glycyrrhizae 20%.
The preparation method of above-mentioned defying age vegetable composition extract, it is characterised in that: above-mentioned vegetable composition is pulverized,
Mix homogeneously after sieving, is that 5:1-30:1 adds Extraction solvent, after soaking 1-3h, at 20-80MPa pressure by liquid material mass ratio
Lower high pressure homogenization method machine carries out extract at room temperature, and extracting solution is performing centrifugal separation on, after membrance concentration, by macroporous resin adsorption, use body
Fraction is the ethanol elution of 10-95%, collects eluent, and membrance concentration to extracting solution is 1:0.1-1 with the mass ratio of Chinese medicine:
10, the butanediol adding and extracting concentrated solution equal in quality dissolves, and filters, and prepares vegetable composition；The present invention uses
Extraction solvent can use water, ethanol, ethanol water.
The second object of the present invention is to be used in cosmetics by above-mentioned anti-defying age vegetable composition, make stable in properties,
Respond well cosmetics.
For realizing the second object of the present invention, present invention employs following technical scheme: be used for by above-mentioned vegetable composition
Preparing cosmetics, it is characterised in that containing above-mentioned vegetable composition in cosmetics, its consumption is calculated as 0.01-25% with mass fraction,
Preferable amount is 1-10%.
Beneficial effects of the present invention: the present invention carries out the design of vegetable composition according to the theory of Chinese medical science of monarch.This
Radix Polygoni Multiflori in the vegetable composition of invention is monarch drug, and the Song dynasty " Kaibao Bencao " is referred to as " to take long muscles and bones, benefit marrow for a long time, prolong life not
Always ".Modern study finds, Radix Polygoni Multiflori can promote the growth of neurocyte and good antioxidation；Fructus Lycii is ministerial drug,
Shennong's Herbal claims Fructus Lycii " the hard muscles and bones of clothes for a long time, makes light of one's life by commiting suicide not old, resistance to cold and summer-heat ", and modern study shows lycium barbarum polysaccharide (LBP) energy
Significantly improve multiplication capacity and the immunity of cell, thus assist Radix Polygoni Multiflori to promote and promote cell growth；Dendrobium nobile is adjuvant drug,
Dendrobium nobile is the rare Chinese medicine that China is traditional, is classified as the top grade of YIN nourishing qi-restoratives in " Sheng Nong's herbal classic ", and modern study shows
Dendrobium nobile can significantly improve superoxide dismutase (SOD) level, reduces lipid peroxide (LPO), removes free radical, plays
The effect of slow down aging；Radix Glycyrrhizae for making medicine, coordinating the actions of various ingredients in a prescription, there is heat-clearing and toxic substances removing, skin-active granulation promoting and whitening, anti-inflammatory efficacy.Above
All medicines share, Synergistic, have and well promote that cell grows, removes free radical, improves skin microcirculation, whitening and raising
Effect of skin elasticity, thus reach skin anti-aging purpose..
Detailed description of the invention
The present invention will be described in more detail to enumerate embodiment below, but the technical scope of the present invention is not implemented by these
Example limits.The most all proportional quantities are the most by weight percentage.
Embodiment 1 vegetable composition extract (I) and preparation method
Plant material Radix Polygoni Multiflori 4kg, Fructus Lycii 2.5kg, Dendrobium nobile 2kg, Radix Glycyrrhizae 1.5kg is weighed by proportioning；By above-mentioned plant group
Compound is pulverized, and sieves, and is the ethanol extraction solvent that 15:1 adds that volume fraction is 50% by liquid material mass ratio, after soaking 2h,
Extract at room temperature is carried out with high pressure homogenization method machine under 50MPa pressure, after extracting solution is performing centrifugal separation on, big by AB-8 type after membrance concentration
Hole resin absorption, is respectively three eluting of ethanol of 50%, 75%, 90% by volume fraction, collects eluent, and membrance concentration is to extracting
Liquid 5kg, adds 5kg butanediol and dissolves, filter, prepare 10kg vegetable composition.
Embodiment 2 vegetable composition extract (II) and preparation method
Plant material Radix Polygoni Multiflori 3.8kg, Fructus Lycii 2.2kg, Dendrobium nobile 2kg, Radix Glycyrrhizae 2kg is weighed by proportioning；By above-mentioned plant group
Compound is pulverized, and sieves, and is the ethanol extraction solvent that 15:1 adds that volume fraction is 40% by liquid material mass ratio, after soaking 2h,
Extract at room temperature is carried out with high pressure homogenization method machine under 50MPa pressure, after extracting solution is performing centrifugal separation on, big by AB-8 type after membrance concentration
Hole resin absorption, is respectively twice eluting of ethanol of 50%, 80% by volume fraction, collects eluent, and membrance concentration is to extracting solution
5kg, adds 5kg butanediol and dissolves, filter, prepare 10kg vegetable composition.
Embodiment 3 vegetable composition extract (III) and preparation method
Plant material Radix Polygoni Multiflori 3.5kg, Fructus Lycii 2.5kg, Dendrobium nobile 2kg, Radix Glycyrrhizae 2kg is weighed by proportioning；By above-mentioned plant group
Compound is pulverized, and sieves, and is the ethanol extraction solvent that 12:1 adds that volume fraction is 60% by liquid material mass ratio, after soaking 2h,
Extract at room temperature is carried out with high pressure homogenization method machine under 40MPa pressure, after extracting solution is performing centrifugal separation on, big by AB-8 type after membrance concentration
Hole resin absorption, is respectively twice eluting of ethanol of 60%, 90% by volume fraction, collects eluent, and membrance concentration is to extracting solution
5kg, adds 5kg butanediol and dissolves, filter, prepare 10kg vegetable composition.
Embodiment 4 vegetable composition extract (IV) and preparation method
Plant material Radix Polygoni Multiflori 3.5kg, Fructus Lycii 2kg, Dendrobium nobile 2.5kg, Radix Glycyrrhizae 2kg is weighed by proportioning；By above-mentioned plant group
Compound is pulverized, and sieves, and is the ethanol extraction solvent that 20:1 adds that volume fraction is 30% by liquid material mass ratio, after soaking 2h,
Extract at room temperature is carried out with high pressure homogenization method machine under 50MPa pressure, after extracting solution is performing centrifugal separation on, big by AB-8 type after membrance concentration
Hole resin absorption, is respectively three eluting of ethanol of 50%, 70%, 90% by volume fraction, collects eluent, and membrance concentration is to extracting
Liquid 5kg, adds 5kg butanediol and dissolves, filter, prepare 10kg vegetable composition.
The preparation of comparative example 1-4 single plant extraction liquid
Weigh plant material Radix Polygoni Multiflori 10kg, Fructus Lycii 10kg, Dendrobium nobile 10kg, Radix Glycyrrhizae 10kg respectively, by above-mentioned plant society
Thing is pulverized, and sieves, and is the ethanol extraction solvent that 15:1 adds that volume fraction is 50% by liquid material mass ratio, after soaking 2h,
Extract at room temperature is carried out with high pressure homogenization method machine under 50MPa pressure, after extracting solution is performing centrifugal separation on, big by AB-8 type after membrance concentration
Hole resin absorption, is respectively three eluting of ethanol of 50%, 75%, 90% by volume fraction, collects eluent, and membrance concentration is to extracting
Liquid 5kg, add 5kg butanediol dissolve, filter, prepare 10kg Radix Glycyrrhizae extract, Radix Polygoni Multiflori extracting solution, Herb Gynostemmae Pentaphylli extract,
Fructus lycii extracted solution, Dendrobium nobile extracting solution.
Embodiment 5 defying age sleep mask
Table 1 provides the defying age sleep mask formula of the vegetable composition extract containing the present invention.
Table 1
| Composition | Mass fraction (%) |
| Cyclomethicone | 3.00 |
| Ring five polydimethylsiloxane | 1.00 |
| Vitamin E | 0.20 |
| Hydrolecithin | 1.20 |
| Carbopol 940 (1% aqueous solution) | 30.00 |
| Ammonium acryloyldime-thyltaurate/VP copolymer | 0.30 |
| Allantoin | 0.10 |
| DL-Panthenol | 0.50 |
| Hyaluronate sodium | 0.07 |
| Deionized water | Surplus |
| Glycerol | 3.00 |
| 1.3-butanediol | 5.00 |
| Methyl hydroxybenzoate | 0.12 |
| Propylparaben | 0.05 |
| Aminomethyl propanol | 0.18 |
| Essence | 0.05 |
| Vegetable composition (I) | 5.00 |
Preparation method:
1, by Oil soluble materials stirring and dissolving in advance to being uniformly dispersed, for oil phase, standby.
2, putting into water-soluble material in deionized water, be heated to 75 ~ 85 DEG C, stirring is to being uniformly dissolved, for aqueous phase, standby
With.
3, oil phase (1000 ~ 2000rpm) under agitation is slowly at the uniform velocity joined in aqueous phase, after addition, all
Reemulsification 5 ~ 10 minutes under matter speed 4000 ~ 6000rpm.
4, it is cooled to 40 DEG C, adds vegetable composition (I) and essence, then add aminomethyl propanol regulation pH value to 5.5 ~ 6.5.
5, stir, be cooled to 35 DEG C, repetition measurement pH final vacuum deaeration, filter discharging,.
Comparative example 5 normal sleep facial film
Table 2 provides the anti-normal sleep face pack prescription of the vegetable composition extract not containing the present invention.
Table 2
| Composition | Mass fraction (%) |
| Cyclomethicone | 3.00 |
| Ring five polydimethylsiloxane | 1.00 |
| Vitamin E | 0.20 |
| Hydrolecithin | 1.20 |
| Carbopol 940 (1% aqueous solution) | 30.00 |
| Ammonium acryloyldime-thyltaurate/VP copolymer | 0.30 |
| Allantoin | 0.10 |
| DL-Panthenol | 0.50 |
| Hyaluronate sodium | 0.07 |
| Deionized water | Surplus |
| Glycerol | 3.00 |
| 1.3-butanediol | 5.00 |
| Methyl hydroxybenzoate | 0.12 |
| Propylparaben | 0.05 |
| Aminomethyl propanol | 0.18 |
| Essence | 0.05 |
Preparation method:
1, by Oil soluble materials stirring and dissolving in advance to being uniformly dispersed, for oil phase, standby.
2, putting into water-soluble material in deionized water, be heated to 75 ~ 85 DEG C, stirring is to being uniformly dissolved, for aqueous phase, standby
With.
3, oil phase (1000 ~ 2000rpm) under agitation is slowly at the uniform velocity joined in aqueous phase, after addition, all
Reemulsification 5 ~ 10 minutes under matter speed 4000 ~ 6000rpm.
4, it is cooled to 40 DEG C, adds aminomethyl propanol regulation pH value to 5.5 ~ 6.5.
5, stir, be cooled to 35 DEG C, repetition measurement pH final vacuum deaeration, filter discharging,.
Experimental example 1 extract anti-senescence function in-vitro evaluation
1, the preparation of sample
Monotaste plant extract prepared by the vegetable composition extract (I) embodiment 1 prepared and comparative example 1-5 is as sample
Solution, carries out in-vitro evaluation experiment.
2, the mensuration of tryrosinase vigor is suppressed
It is loaded according to table 3, solution is placed in constant temperature 10min in 30 DEG C of water-baths；Add tryrosinase, solution is placed in 30 DEG C of water-baths
After middle constant temperature 10min, under 475nm, measure its absorbance.
Table 3
| Project | Blank (ml) | Blank (ml) | Sample controls (ml) | Sample (ml) |
| Sample | - | - | 1.0 | 1.0 |
| PBS(pH=6.8) | 2.5 | 2.0 | 1.5 | 1.0 |
| TYR | 1.0 | 1.0 | 1.0 | 1.0 |
| Tryrosinase | - | 0.5 | - | 0.5 |
| Total amount | 3.5 | 3.5 | 3.5 | 3.5 |
By formula (1) calculate sample to the relative inhibition of tryrosinase (I, it is called for short suppression ratio, lower same).
I=[(A1-A2)-(A3-A4)] × 100% ÷ (A1-A2) (1)
In formula: A1, A2, A3, A4 are respectively the absorbance of 1 group～4 groups solution.
3, the mensuration of ultra-oxygen anion free radical scavenging action
Take the Tris-HCl buffer 4.5mL of 0.05mol/L pH=8.2, in 25 DEG C of water-baths, preheat 20min.Add 1mL
The pyrogallol solution of sample and 0.4mL 25mmol/L, after mixing, reacts 5min in 25 DEG C of water-baths, adds 8mol/L's
HCl 1.0mL terminates reaction.Make reference with Tris-HCl buffer, at 299nm, survey absorbance.Blank 1mL tries
The solvent of sample substitutes sample.Clearance rate (D) is calculated according to formula (2).
·O2Clearance rate=[1-(A6/ A5)] × 100% (2)
In formula, A5 is the absorbance of blank；A6 is the absorbance of medicine.
4, the Scavenging activity of hydroxy radical is measured
2mmol/L FeSO it is sequentially added in 25mL color comparison tube43mL, 1mmol/L H2O23mL, shakes up, and is subsequently added into
6mmol/L salicylic acid 3mL, shakes up, and takes out, survey its absorbance after 37 DEG C of heating in water bath 15min；It is separately added into certain density
Liquid to be measured, shakes up, and continues heating in water bath 15min, takes out and surveys its absorbance.Following formula is clear to carboxylic free radical (OH) of liquid to be measured
Except rate:
OH clearance rate (%)=[A7-A8-(A7-A9)] A7 × 100% (3)
In formula, A7 is not add the absorbance of reaction system before medicine；A8 is that the absorbance of system after OH removed by medicine；A9
For the absorbance of system after blank removing OH.
5, experimental result
Anti-senescence function evaluation experimental is as shown in table 4.
Table 4 experimental result
Experiment shows, the vegetable composition of the present invention can effectively suppress tyrosinase activity and remove the effect of free radical, and
And also stronger than the effect of single plant extract, illustrate to serve synergistic function after compound recipe.
Experimental example 2 defying age sleep mask effect human body evaluation test
1, experimental technique
The sleep mask of embodiment 3 and comparative example 6 preparation carries out normal individual trial evaluation respectively, according to " cosmetics safety technology is advised
Model " (version in 2015) test.Choose the healthy, without volunteer 40 (10 men of cosmetic allergic contact dermatitis history of 30-50 year
Property, 30 women).Using method is use after face cleaning every day, and once a day (before sleep in evening), left face uses by embodiment 5
The defying age sleep mask of preparation, right face uses the normal sleep facial film by comparative example 5 preparation.Experiment periods is one month, periodically
Measure the skin elasticity of volunteer face and dermal melanin, and after using one month the colour of skin to left face and right face carry out right
Than observing.
2, experimental result
To the face of volunteer it has been observed that have that 31 user employ by embodiment 4 preparation is anti-ageing after using one month
The colour of skin of the left face of old sleep mask significantly better than the colour of skin of right face of the normal sleep facial film employed by comparative example 5 preparation, 9
People is not clearly distinguished from, and effective percentage reaches 77.5%.
Claims (2)
1. a vegetable composition with senile-resistant efficacy, it is characterised in that be made up of the component of following percentage by weight: what
Radix Polygoni Multiflori 10-50%, Fructus Lycii 5-35%, Dendrobium nobile 5-35%, Radix Glycyrrhizae 5-30%；The manufacture method of vegetable composition is: plant above-mentioned
Compositions is pulverized, mix homogeneously after sieving, and is that 5:1 ~ 30:1 adds Extraction solvent by liquid material mass ratio, after soaking 1 ~ 3h,
Carrying out extract at room temperature with high pressure homogenization method machine under 20 ~ 80MPa pressure, extracting solution is performing centrifugal separation on, after membrance concentration, with macropore tree
Fat adsorbs, and is the ethanol elution of 10 ~ 95% by volume fraction, collects eluent, and membrance concentration to extracting solution with the mass ratio of Chinese medicine is
1:0.1 ~ 1:10, the butanediol adding and extracting concentrated solution equal in quality dissolves, and filters, and prepares vegetable composition；The present invention
The Extraction solvent of middle use can use water, ethanol, ethanol water.
2. a kind of described in claim 1 has the application in cosmetics of the vegetable composition of senile-resistant efficacy, and its feature exists
Containing the vegetable composition described in claim 1 in cosmetics, its consumption is calculated as 0.01-25% with mass fraction.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107137316A (en) * | 2017-05-25 | 2017-09-08 | 海南诗博丽生物科技有限公司 | A kind of stem of noble dendrobium lip gloss and preparation method thereof |
| CN108309841A (en) * | 2018-04-10 | 2018-07-24 | 广州赛莱拉干细胞科技股份有限公司 | Skin care compositions based on China fir leaf caulerpa extract and its application |
| CN113413329A (en) * | 2021-08-02 | 2021-09-21 | 遵义医科大学 | Dendrobium nobile polysaccharide herbal face cream and preparation method thereof |
| WO2023226346A1 (en) * | 2022-05-27 | 2023-11-30 | 广州环亚化妆品科技股份有限公司 | Anti-aging plant extract composition, preparation method therefor and use thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107137316A (en) * | 2017-05-25 | 2017-09-08 | 海南诗博丽生物科技有限公司 | A kind of stem of noble dendrobium lip gloss and preparation method thereof |
| CN107137316B (en) * | 2017-05-25 | 2021-02-26 | 海南诗博丽生物科技有限公司 | Dendrobium lip balm and preparation method thereof |
| CN108309841A (en) * | 2018-04-10 | 2018-07-24 | 广州赛莱拉干细胞科技股份有限公司 | Skin care compositions based on China fir leaf caulerpa extract and its application |
| CN113413329A (en) * | 2021-08-02 | 2021-09-21 | 遵义医科大学 | Dendrobium nobile polysaccharide herbal face cream and preparation method thereof |
| WO2023226346A1 (en) * | 2022-05-27 | 2023-11-30 | 广州环亚化妆品科技股份有限公司 | Anti-aging plant extract composition, preparation method therefor and use thereof |
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