CN106963677B

CN106963677B - Striae gravidarum removing cream

Info

Publication number: CN106963677B
Application number: CN201710182413.XA
Authority: CN
Inventors: 吴倩; 诸辉
Original assignee: Ningbo Xinuoya Marine Biotechnology Co ltd
Current assignee: Ningbo Xinuoya Marine Biotechnology Co ltd
Priority date: 2017-03-24
Filing date: 2017-03-24
Publication date: 2021-02-26
Anticipated expiration: 2037-03-24
Also published as: CN106963677A

Abstract

The invention discloses stretch mark removing cream which comprises the following raw materials in percentage by weight: 50-70% of beta-glucan, 3-5% of glycerol, 2-5% of jojoba seed oil, 2-5% of vitamin E oil, 2-5% of shea butter, 3-7% of olive oil, 1-3% of centella asiatica extract, 0.2-1% of notoginsenoside and 0.1-1% of dipotassium glycyrrhizinate. The invention has the advantages that through reasonable formula combination, each effective component can be quickly introduced into the dermis of the skin to accelerate skin repair, so that the collagen fibers and the elastic fibers of the skin forming striae gravidarum are effectively repaired, simultaneously pigment is lightened, the moisturizing effect on the skin is enhanced, and the formed striae gravidarum, especially striae gravidarum of more than 3 years, is good in removing effect.

Description

Striae gravidarum removing cream

Technical Field

The invention relates to a skin care product, in particular to stretch mark removing cream.

Background

The mechanisms by which the skin folds are mainly 2, one is intrinsic aging and the other is extrinsic damage. The formation of striae gravidarum is attributed to the latter, which is caused by the damage or rupture of elastic and collagen fibers of the skin due to the influence of pregnancy hormones and abdominal distension. At present, products for removing striae gravidarum are more, for example, Chinese patent CN103655355A discloses a skin lotion for removing striae gravidarum, which comprises, by mass, 5-10% of tremella extract, 2-10% of centella asiatica extract, 1-5% of beeswax, 2-5% of lanolin, 5-15% of vaseline, 1-4% of sorbitan sesquioleate, 5-10% of glycerol and 50-80% of purified water, and can repair skin and improve striae gravidarum in time, but the skin lotion is an oil-in-water system, has poor skin moistening performance and limited effect on improving striae gravidarum; chinese patent CN106361608A discloses a pure natural striae gravidarum removing composition containing camellia oil, which comprises the following raw materials in percentage by weight: 0.1-0.5% of lavender alcohol, 0.01-0.02% of jasmonate, 0.03-0.06% of rosemary diterpene phenol and 99.387-99.849% of camellia oil, and the lavender alcohol is an oily substrate which can have better moistening degree but can cause sticky feeling when being used and has poor experience; chinese patent CN106281808A discloses a soap for fading striae gravidarum, which comprises the following raw materials in parts by weight: 2 to 6 portions of tea oil, 3 to 6 portions of tomato juice, 1 to 3 portions of pine pollen, 1 to 3 portions of lemon juice and 80 to 100 portions of soap base, which belong to soap products and can not effectively play a role of removing striae gravidarum when staying on the skin for a short time.

The products belong to oily substrates, so that the use experience is poor, the soap base cannot play a role in effectively removing the striae gravidarum when the soap base is used for a short time on the skin, the striae gravidarum removing effect is weak or ineffective when the soap base is used for a long time, such as more than 3 years, or the absorption effect is poor.

Disclosure of Invention

In order to solve the technical problems, the invention provides stretch mark removing cream for fading and removing stretch marks and fat marks.

The invention provides stretch mark removing cream, which comprises the following raw materials in percentage by weight:

50-70% of beta-glucan, 3-5% of glycerol, 2-5% of jojoba seed oil, 2-5% of vitamin E oil, 2-5% of shea butter, 3-7% of olive oil, 1-3% of centella asiatica extract, 0.2-1% of notoginsenoside and 0.1-1% of dipotassium glycyrrhizinate.

The balance of water, which is less than one hundred percent of the raw materials.

As a further improvement, the stretch mark removing cream comprises the following raw materials in percentage by weight: 50-60% of beta-glucan, 4-5% of glycerol, 2-3% of jojoba seed oil, 4-5% of vitamin E oil, 2-4% of shea butter, 5-7% of olive oil, 2-3% of centella asiatica extract, 0.8-1% of notoginsenoside and 0.1-0.5% of dipotassium glycyrrhizinate.

As a further improvement, the stretch mark removing cream also comprises a preservative, and the preservative comprises 0.1-0.3% of methyl hydroxybenzoate and 0.1-0.5% of bis (hydroxymethyl) imidazolidinyl urea.

The beta-glucan is obtained according to the method described in Chinese patent application CN 201510725996.7.

The preparation method of the stretch mark removing cream comprises the following steps:

s1, weighing the raw materials in percentage by weight;

s2, mixing jojoba seed oil, vitamin E oil, shea butter and olive oil at 70-85 deg.C, stirring for 20-35min to obtain oil phase, and keeping the temperature;

s3, mixing beta-dextran, glycerol, herba Centellae extract, notoginsenoside, dipotassium glycyrrhizinate, and water at 70-85 deg.C, stirring for 25-35min to obtain water phase, and keeping the temperature;

s4, adding the water phase obtained in the step S3 into the oil phase obtained in the step S2, homogenizing for 7-15min, cooling to 50-60 ℃, and homogenizing for 2-5 min.

The step S4 further includes adding a preservative after cooling.

The invention has the advantages and beneficial effects that:

1. the extract is prepared from natural sources as main effective component

The beta-glucan is extracted by fermenting the schizophyllum commune, can improve the proliferation rate of skin cells, promote the generation of collagen and elastin, help to renew and repair damaged skin, promote scar healing, simultaneously can keep skin moisture, improve the moisture retention and tightening smoothness of the skin, and can inhibit mast cells from releasing histamine and degranulation to relieve sensitive skin;

the centella asiatica extract has the effects of promoting skin collagen regeneration, enhancing cell basal layer cell activity, and keeping skin elasticity and compactness; lightening melanin deposition, and renewing skin cells; increasing skin water retention degree, and activating and renewing skin cells.

The panax notoginseng saponins are effective components extracted and separated from the panax notoginseng, comprise more than 20 saponin active substances, 17 trace elements, protein, abundant vitamins, polysaccharide and the like, have the effects of eliminating free radicals, resisting inflammation and oxidation, improving the blood circulation and metabolism of local skin, enhancing the immunity of the skin, accelerating wound healing and repairing the damaged skin of inflammation;

the jojoba seed oil contains abundant vitamin A, B, E and minerals such as calcium, magnesium and the like, has a composition close to skin grease, and can provide two layers of effects on skin, namely, the jojoba seed oil can rapidly permeate and absorb and soften skin cutin to enable the skin to become soft and elastic;

the olive oil contains more vitamins than other oils, such as vitamin A (and provitamin A) with effects of smoothing and tenderizing skin and increasing its elasticity, vitamin B1 with function of smoothing skin fine wrinkles, and vitamin C with effects of enhancing blood vessel wall elasticity and expanding blood flow; the vitamin E oil is matched with the activity of inhibiting tyrosinase, so that the generation of melanin can be effectively reduced;

the shea butter has the effects of promoting skin regeneration and preventing photoaging, and has obvious healing promoting effect on skin with dermatitis, sunburn and scars. Has good long-term moisturizing effect on the surface layer of the skin, has good spreadability and transdermal absorbability, is particularly suitable for a formula without greasy, relieves irritation and moisturizes the surface layer.

2. The preparation method has the advantages that the beta-glucan with a specific source is selected as a main matrix, and the percutaneous absorption effect of effective components, particularly the centella asiatica extract, can be increased through the reasonable proportion of the components, so that the components in the stretch mark removing cream can be effectively and quickly absorbed by the skin, and are led into the dermal layer part of the skin to repair broken collagen fibers and elastic fibers, the repair function of the skin is reactivated, the stretch marks with long forming time can be eliminated, the pigment is lightened, the moisturizing effect on the skin is enhanced, and the effect of removing the stretch marks is further achieved; on the other hand, the beta-glucan plays a role in emulsification, and the use of an emulsifier is reduced.

Drawings

Figure 1 is a graph of the amount of transdermal absorption of a β -glucan carrier-centella asiatica extract and a single centella asiatica extract as a function of time.

Detailed Description

The present invention will be further described with reference to the following embodiments.

Example 1

The invention provides stretch mark removing cream which comprises the following raw materials in percentage by weight:

50% of beta-glucan, 5% of glycerol, 5% of jojoba seed oil, 2% of vitamin E oil, 5% of shea butter, 5% of olive oil, 1% of centella asiatica extract, 0.3% of notoginsenoside, 0.1% of dipotassium glycyrrhizinate and the balance of water.

s1, weighing the raw materials in percentage by weight;

Example 2

70% of beta-glucan, 3% of glycerol, 2% of jojoba seed oil, 5% of vitamin E oil, 4% of shea butter, 7% of olive oil, 3% of centella asiatica extract, 1% of notoginsenoside, 1% of dipotassium glycyrrhizinate and the balance of water.

The preparation method is the same as example 1.

Example 3

55% of beta-glucan, 4% of glycerol, 3% of jojoba seed oil, 5% of vitamin E oil, 4% of shea butter, 4% of olive oil, 1% of centella asiatica extract, 0.5% of notoginsenoside, 0.3% of dipotassium glycyrrhizinate and the balance of water.

The preparation method is the same as example 1.

Example 4

60% of beta-glucan, 5% of glycerol, 4% of jojoba seed oil, 3% of vitamin E oil, 3% of shea butter, 3% of olive oil, 2% of centella asiatica extract, 0.8% of notoginsenoside, 0.8% of dipotassium glycyrrhizinate and the balance of water.

The preparation method is the same as example 1.

Example 5

beta-glucan 65%, glycerin 5%, jojoba seed oil 5%, vitamin E oil 4%, shea butter fruit resin 2%, olive oil 3%, centella asiatica extract 1.5%, notoginsenoside 0.6%, dipotassium glycyrrhizinate 0.6%, methylparaben 0.1%, bis (hydroxymethyl) imidazolidinyl urea 0.3%, and the balance of water. The methylparaben and the bis (hydroxymethyl) imidazolidinyl urea can be used as a preservative to prolong the shelf life of the product, further improve the coatability and smoothness of the product, and improve the use feeling of the product.

s1, weighing the raw materials in percentage by weight;

s4, adding the water phase obtained in the step S3 into the oil phase obtained in the step S2, homogenizing for 7-15min, cooling to 50-60 ℃, adding methylparaben and bis (hydroxymethyl) imidazolidinyl urea, and homogenizing for 2-5 min.

Example 6

beta-glucan 50%, glycerin 5%, jojoba seed oil 4%, vitamin E oil 3%, shea butter fruit resin 2%, olive oil 3.5%, centella asiatica extract 1%, notoginsenoside 0.4%, dipotassium glycyrrhizinate 0.4%, methylparaben 0.2%, bis (hydroxymethyl) imidazolidinyl urea 0.4%, and the balance of water. The methylparaben and the bis (hydroxymethyl) imidazolidinyl urea can be used as a preservative to prolong the shelf life of the product, further improve the coatability and smoothness of the product, and improve the use feeling of the product.

The preparation method is the same as example 5.

The beta-glucan used in the above examples was prepared by the method described in chinese patent application CN 201510725996.7.

Effect evaluation test of Components and products of the invention

1. Transdermal absorption experiment of beta-glucan carrier-centella asiatica extract system by adopting in-vitro skin assay method

The sample solution is beta-glucan-centella asiatica extract (mass ratio is 49:1)50g dissolved in 200ml of normal saline, and 1g of centella asiatica extract dissolved in 200ml of normal saline; physiological saline was used as the receiving solution.

Shaving the dead abdomen of the mouse after neck breaking, shearing the skin of the abdomen, and repeatedly washing the belly with normal saline for later use; before the experiment, the integrity of the rat skin is checked visually, and no damage can be caused; fixing the stratum corneum of the in vitro skin of the mouse on a Franz diffusion cell upwards, and filling water in a supply cell and ensuring the sealing of the supply cell; respectively adding the receiving solution into a receiving pool and 2ml of sample solution into a supply pool by using an injector; taking 1ml of receiving solution at different time intervals for HPLC determination, and supplementing an equal amount of fresh receiving solution, wherein the determination method comprises evaporating the receiving solution to dryness, re-dissolving with methanol, centrifuging at 13000 r/min for 15min, and taking supernatant for HPLC determination. After the skin penetration is finished, cutting off the rat skin of the part of the skin penetration area, washing the skin penetration surface with purified water, wiping the surface of the rat skin with an alcohol cotton ball to remove the residual medicine on the surface, cutting up the rat skin, placing the cut rat skin into a centrifugal tube containing 1mL of methanol, performing ultrasonic extraction for 60min, centrifuging at 13000 rpm for 15min, taking supernatant, and performing HPLC sample injection detection to determine the skin retention of the sample. The results of transdermal absorption are shown in Table 1

TABLE 1

From Table 1, it can be seen that the transdermal accumulation rate and amount of the same concentration of the centella asiatica extract solution in the presence of the beta-glucan carrier at the same time interval are much higher than those of a single centella asiatica extract solution.

2. Evaluation of repair effect of striae gravidarum removing cream on damaged skin

The method comprises the following steps: testing of cytotoxicity

The samples were diluted with serum-free DMEM medium to five concentrations of 500mg/ml, 250mg/ml, 125mg/ml, 72.5mg/ml and 36.25mg/ml, and 10% newborn calf serum was added. Cell suspension was prepared by conventional fibroblast culture and seeded in 96-well cell culture plates with 5% CO₂After incubation at 37 ℃ for 24h, the stock culture was discarded and 125. mu.l of each well was added with the test samples at different concentrations and negative controls. The incubator is incubated for 48h +/-0.5 h (5% CO)₂37 ℃, relative humidity > 90%). And (3) observing the cell morphology and characteristics under a phase contrast inverted microscope, and detecting the cell activity by using an MTT method. IC50 was calculated from the concentration-response relationship using the log-probability unit method.

Step two: collagen and elastin synthesis assay

According to the cytotoxicity test result in the step one, selecting the concentration of the test object with the cell activity of more than 50% (IC50) as the initial concentration of the efficacy test, and diluting the test object to three concentrations of 120mg/ml, 100mg/ml and 83.3mg/ml by taking DMEM culture solution containing 10% NCS as a dilution factor; negative control group (NC, no glucocorticoid) and positive control group (PC, glucocorticoid exposure) are provided. Fibroblast cell size of 1.0 × 10⁵The culture dish is inoculated with/ml of the culture dish and cultured for 24 hours to form a monolayer of cells; after the cells were exposed to glucocorticoid (10. mu.g/(g medium)) for 24 hours by replacing the original medium with HBSS, they were washed 2 times with PBS, and the medium containing the test substance at different concentrations was added to continue the culture for 24 hours. Cells were scraped off into PBS and cell contents were collected by centrifugation and sonication and stored at-20 ℃.

The elastin content is indirectly determined by the activity of elastase, and the two are in negative correlation: preheating a 96-well plate at 37 ℃, incubating a sample to be detected and a substrate STANA of elastase for 1h at 37 ℃, and measuring absorbance at the wavelength of 410 nm; elastase activity was expressed as a percentage with NC as a control.

Collagen content was determined directly using the type I procollagen C-peptide (PIP) Elisa kit (Takara Bio Inc.) and the type I procollagen C-peptide content was expressed as a percentage with the NC group as a control.

The following judgment criteria are met, and the test substance has the effect of promoting the generation of elastin and collagen:

compared with a positive control group, the reduction of the elastase activity of the experimental group has significant significance (statistical significance, P is less than 0.05);

② compared with the positive control group, the increase of the collagen content in the experimental group has significant meaning (statistical meaning, P is less than 0.05). The results are shown in Table 2

TABLE 2

*: the difference in comparison with the NC group has statistical significance

Compared with a positive control, the test substance of 120mg/ml can obviously reduce the activity of the elastase (P <0.01) and obviously improve the content of procollagen (P < 0.01). The above results indicate that the sample has the effect of promoting the production of elastin and collagen in a certain concentration range for human primary fibroblasts.

The comparison of the using effect of the stretch mark removing cream of the invention and the use effect of certain commercially available stretch mark removing cream (the components are pure cocoa butter, vitamin E, elastin, collagen and shea butter) is shown in Table 3

TABLE 3

As can be seen from Table 3, the stretch mark removing cream has a good stretch mark removing effect, and particularly has an obvious effect on stretch marks of more than 3 years compared with the existing products.

Materials, reagents and experimental equipment related to the embodiment of the invention are all commercial products which accord with the field of manufacturing and selling skin care cosmetics unless particularly stated.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, modifications and decorations can be made without departing from the core technology of the present invention, and these modifications and decorations shall also fall within the protection scope of the present invention. Any changes which come within the meaning and range of equivalency of the claims are to be embraced within their scope.

Claims

1. The stretch mark removing cream comprises the following raw materials in percentage by weight:

50-70% of beta-glucan, 3-5% of glycerol, 2-5% of jojoba seed oil, 2-5% of vitamin E oil, 2-5% of shea butter, 3-7% of olive oil, 1-3% of centella asiatica extract, 0.2-1% of notoginsenoside and 0.1-1% of dipotassium glycyrrhizinate; the beta-glucan is obtained by fermenting and extracting schizophyllum commune; also comprises a preservative, wherein the preservative comprises 0.1-0.3% of methyl hydroxybenzoate and 0.1-0.5% of bis (hydroxymethyl) imidazolidinyl urea.

2. The stretch mark removing cream according to claim 1, characterized in that the preparation method comprises:

S1weighing the raw materials in percentage by weight;

S2mixing jojoba seed oil, vitamin E oil, shea butter fruit resin and olive oil at 70-85 deg.C, stirring for 20-35min to obtain oil phase, and keeping the temperature;

S3mixing beta-dextran, glycerol, herba Centellae extract, notoginsenoside, and dipotassium glycyrrhizinate at 70-85 deg.C, stirring for 25-35min to obtain water phase, and keeping the temperature;

will be described in the specificationS3The resulting aqueous phase is added to stepS2Homogenizing the obtained oil phase for 7-15min, cooling to 50-60 deg.C, and homogenizing for 2-5 min;

s4, adding the water phase obtained in the step S3 into the oil phase obtained in the step S2, homogenizing for 7-15min, cooling to 50-60 ℃, adding a preservative, and homogenizing for 2-5 min.