KR101666348B1

KR101666348B1 - Compositions for improving skin wrinkle or enhancing skin elasticity comprising Cynanchum wilfordii and composition prepared therefrom

Info

Publication number: KR101666348B1
Application number: KR1020150113930A
Authority: KR
Inventors: 김수경; 서동구; 차영주
Original assignee: 김수경; 서동구; 차영주
Priority date: 2015-08-12
Filing date: 2015-08-12
Publication date: 2016-10-17

Abstract

The present invention relates to a method for manufacturing a cosmetic composition for reducing skin wrinkles or enhancing skin elasticity using naturally-derived ingredients including Cynanchum wilfordii Hemsley and the cosmetic composition manufactured thereby. The method comprises the following steps: (A) mixing Cynanchum wilfordii Hemsley, one or more ingredients selected from the group consisting of Rubus coreanus fruits, Cuscuta chinensis Lamark seeds, Cnidium monnieri (L). Cussion fruits, Lycium chinense Miller fruits, Schisandra chinensis fruits, Dioscorea batatas Decaisne roots, and acanthopanax, brown rice, leaven, grain syrup, effective microorganisms, and concentrated mineral water and then adding deep sea water and loess jijangsu, before primarily fermenting the same; (B) injecting air under the same temperature condition as the primary fermentation and fermenting secondarily; (C) mixing Cynanchum wilfordii Hemsley, Astragalus membranaceus BUNGE, one or more ingredients selected from the group consisting of Cistanche deserticola Y. C. Ma, Morinda officinalis roots, Eucommia ulmoides OLIVER, Polygala tenuifolia Willdenow, Phyllostachyos caulis in Taeniam, Acorus graminens SOLAND, Rehmanneh glutinosa Libosch. var. purpurea Mak., Poria cocas Wolf., Polydonati rhizoma, Cinnamomum cassia Presl, red ginseng, Inonotus obliquus, pine nuts, and walnuts, one or more ingredients selected from the group consisting of licorice and Lycium chinense Miller fruits, concentrated mineral water, and deep sea water, and preparing an extract by hot water extraction; (D) separating the product fermented at the step (B) into a fermented liquid and a residue by filtration; (E) preparing an extract from the residue separated at the step (D) by hot water extraction; and (F) mixing the fermented liquid separated at the step (D), the extract extracted at the step (E), and the extract extracted at the step (C). Therefore, the cosmetic composition can enhance skin elasticity, excellently reduce skin wrinkles, and supplement a female hormone in a menopausal woman.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for improving skin wrinkles or skin elasticity by using a naturally-occurring material including white sachet, and a cosmetic composition prepared thereby,

The present invention relates to a method for producing a cosmetic composition for improving skin wrinkle or skin elasticity using a natural-derived material including white mulberry, which not only has excellent skin wrinkle improving effect but also produces female hormone, and To a cosmetic composition prepared thereby.

Because the skin has many opportunities to interact with various external stimuli, it is more prone to wrinkles than other organs. Among them, facial skin is directly exposed to sunlight, dry outside air and pollutants, so that aging phenomenon such as wrinkles occurs more early than other skin.

The most characteristic change due to aging of the skin tissue is a change in the skin matrix, which causes aging of the human skin fibroblast in the dermis, resulting in a decrease in the ability to produce fibrous and matrix. The amount of the substrate is generally decreased, the thickness of the skin is thinned, and the elasticity of the skin is lowered to form the wrinkles. That is, as aging progresses, the skin becomes weaker in elasticity, disorder of blood circulation, weakening of skin barrier, and the like.

Collagen, a major component of the extracellular matrix, is a major substrate protein in the fibroblasts of the skin and is present in extracellular epilepsy. It is also an important protein that accounts for about 30% of the total weight of bioproteins, and has a solid triple helix structure.

Collagen forms most of the organic matter in the skin, tendons, bones and teeth, especially in bone and skin (dermis). In most other structures, it exists as a fibrous inclusion.

The collagen is a relatively weak immunogen, partly due to the blocking of the latent antigenic determinant by the helical structure of the collagen, which also causes the collagen to be resistant to proteolysis.

The functions of collagen present in the skin are known as mechanical rigidity of the skin, resistance of connective tissues and binding force of tissues, support of cell adhesion, induction of cell division and differentiation (when organism grows or healing) (van der Rest et al., 1990). Such collagen is reduced by aging and UV irradiation, which is known to be closely related to skin wrinkling and skin elasticity (Arthur K. Balin et al., "Aging and the skin ", 1989). In recent years, as a result of extensive research on skin aging, important functions of collagen in the skin have been revealed.

In the prior art, there have been products that incorporate collagen into cosmetics from the skin moisturizing effect of collagen, but these cosmetics apply collagen to the skin surface, and since the collagen, which is a polymer, can not be expected to be moisturized by transdermal absorption, Can not be said.

As a collagen synthesis promoting substance, retinoic acid, TGF (transforming growth factor carcinogenesis factor; Cardinale G. et al, Adv. Enzymol., 41, p. 425, 1974) -231370), betulinic acid (JP8-208424), chlorella extract (JP9-40523, JP10-36283, fibroblast proliferation promoting action) are known, but when applied to the skin, there are safety problems such as stimulation and redness The use amount is limited or the effect is insignificant, so that the skin collagen synthesis is substantially promoted and the skin function improving effect can not be expected.

Therefore, it is required to develop a composition which is safe in vivo and which is more effective than a substance promoting collagen synthesis performance.

Korean Patent Publication No. 2010-0028601 Korea Patent Publication No. 2009-0081810 Korea Patent Publication No. 2009-0063456

It is an object of the present invention to provide a cosmetic composition for skin wrinkle improvement or skin elasticity enhancement using a naturally occurring substance including white sachet which not only has excellent skin wrinkle improving effect but also produces female hormone Method.

Another object of the present invention is to provide a cosmetic composition prepared by the above-mentioned method.

In order to accomplish the above object, the present invention provides a cosmetic composition for improving skin wrinkles or skin elasticity using a natural derived material comprising white sachet (A) Useful microorganisms such as liquorice, acacia, brown rice, yeast, zucchini, EM solution; Mixing the mineral water with mineral water, adding the deep sea water and yellow clay water to the first fermentation;

(B) injecting air under the same temperature as that of the primary fermentation to perform secondary fermentation;

(C) Mixed with deep seawater, concentrated water of mineral water and deep sea water, pine nut, hwanggi, jaejong yong, paulchiae, mugwort, raw paper, Killer, Seokchangpo, Seokgiwang, Baekbyeong, Hwangjeong, Broiler, Red ginseng, Extracting the hot water;

(D) separating the fermented product fermented in step (B) into a fermentation broth and a residue;

(E) hot water extraction of the residue separated in step (D); And

(F) mixing the fermentation broth separated in step (D), the extract extracted in step (E), and the extract extracted in step (C).

50 to 600 parts by weight of the group consisting of bokbunja, earthworm, casualty, gugija, omija, acidophilus, and ogap is 100 parts by weight in the step (A) 5 to 10 parts by weight of brown rice; 5 to 10 parts by weight of yeast; 100 to 300 parts by weight of crude oil; 100 to 300 parts by weight of useful microorganisms; 20 to 100 parts by weight of mineral concentrated water; 1500 to 2000 parts by weight of deep ocean water; And 1,500 to 2,000 parts by weight of yellow clay.

In the step (A), the primary fermentation may be performed at 20 to 50 ° C for 12 to 20 days.

In the step (B), the secondary fermentation may be performed for 12 to 20 days.

100 to 300 parts by weight of sulfur based on 100 parts by weight of bagasse in step (C); 100 to 3500 parts by weight of a group consisting of green tea, green tea, green tea, green tea, kelp, kochujang, seokjanghwang, white bongryeong, hwangjeong, broiler, red ginseng, pine nut and walnut; 100-400 parts by weight of a group consisting of licorice and coriander; 20 to 50 parts by weight of mineral concentrated water; And deep sea water at 1000 to 20,000 parts by weight.

In the step (C), the hot-water extract may be a mixture obtained by extracting the mixture at 70 to 90 ° C. for 100 to 130 hours.

In the step (E), the hot-water extract may be a mixture of the residue and water at a weight ratio of 1: 1 to 3 and extracted at 70 to 90 ° C for 10 to 30 hours.

In step (F), the fermentation broth separated in step (D), the extract extracted in step (E), and the extract extracted in step (C) are mixed at a weight ratio of 1: 0.2-0.8: 0.5-3.0 .

According to another aspect of the present invention, there is provided a cosmetic composition for improving skin wrinkles or skin elasticity.

The composition may be in the form of a solution, an external ointment, a cream, a foam, a nutritional lotion, a softening longevity pack, a softener, a milky lotion, a makeup base, an essence, a soap, a liquid detergent, , A gel, a lotion, a powder, a soap, a surfactant-containing cleansing, an oil, a powdered foundation, an emulsion foundation, a wax foundation, a patch and a spray.

According to another aspect of the present invention, there is provided a method for manufacturing a skin composition for improving skin wrinkles or skin elasticity, comprising the steps of: (A) preparing a composition for skin wrinkles or skin elasticity, Useful microorganisms such as Nuruk, Chojung and EM solution; Mixing the mineral water with mineral water, adding the deep sea water and yellow clay water to the first fermentation;

(E) hot water extraction of the residue separated in step (D); And

The cosmetic composition for improving skin wrinkles or skin elasticity according to the present invention has an excellent collagen synthesis promoting activity, and therefore can have an effect of improving skin wrinkles or enhancing skin elasticity.

In addition, the cosmetic composition of the present invention can induce postmenopausal menstruation again because it produces female hormone, and can improve the calm feeling after menopause and maintain good mood.

In addition, the composition of the present invention can be applied not only to cosmetics to be applied but also to cosmetics to be eaten, but also to foods.

The present invention relates to a method for producing a cosmetic composition for improving skin wrinkle or skin elasticity using a natural-derived material including white seaweed, which not only has excellent skin wrinkle improving effect but also produces female hormone, And to a cosmetic composition prepared thereby.

In the present invention, "skin wrinkle" refers to a scarring caused by skin damage, which may be caused by a cause of a gene, a decrease in collagen and elastin present in the skin dermis, and an external environment.

In the present invention, "improvement of skin wrinkles" refers to inhibiting or inhibiting the generation of wrinkles on the skin, or alleviating already formed wrinkles.

In the present invention, "skin elasticity" is expressed by elastic fibers composed of elastin existing in the dermal layer. Such elastic fibers have a very low elastic modulus such as rubber and are easily deformed by a small force, When the force is removed, it easily returns to the original shape. In addition, the elastic fibers have a form in which microfibrils are embedded in an amorphous matrix called elastin, and elastin is a protein that is found only in elastic fibers such as desmosine and isodesmosine derived from lysine, It is a protein composed of one amino acid. These desmosins and isodesmesynes form cross-links in long peptide chains, which makes elastin behave like a rubber.

In the present invention, "skin elasticity enhancement" refers to elasticity fibers composed of elastin together with collagen fibers called collagen, wherein skin elasticity is maintained in a state where elastin and collagen are sufficiently present.

Hereinafter, the present invention will be described in detail.

A method for producing a cosmetic composition for improving skin wrinkles or skin elasticity using a naturally-occurring material comprising white sachet of the present invention comprises the steps of: (A) (B) injecting air under the same temperature as that of the primary fermentation to perform secondary fermentation; (C) hot water extraction by mixing white algae and other natural substances; (D) separating the fermented product fermented in step (B) into a fermentation broth and a residue; (E) hot water extraction of the residue separated in step (D); And (F) mixing the fermentation broth separated in step (D), the extract extracted in step (E), and the extract extracted in step (C).

First, in step (A) above, At least one member selected from the group consisting of bokbunja, earthworm, casualty, gojira, omija, mackerel and ogapi; Brown rice; yeast; Cho Chung; Useful microorganisms; After mixing the mineral concentrated water, deep ocean water; And yellow clinging factor are added to the first fermentation.

The above-mentioned Bacillus subtilis is an Asclepiadaceae large gourd (Cynanchum wilfordii Hemsley), and is a root plant and has the efficacy of nourishment, blood, blood (补精) and leiomyoma (leukemia) It is used for white hair (early white hair), nervous breakdown, chronic airflow (chronic wind).

When at least one selected from the group consisting of brambles, earthworms, casualties, gougie, omija, liquorice, and ogapi is fermented together with white seaweed, it can improve skin wrinkles, improve skin elasticity, It uses bokbunja, tosajeon, casualties, gogija, omija, mackerel and ogapi.

At least one selected from the group consisting of brambles, dead animals, casualties, gypsum, omija, liquorice, and acacia is used in an amount of 50 to 600 parts by weight based on 100 parts by weight of white mud. 1: 1: 1: 1: 1. If the content of at least one selected from the group consisting of bokbunja, skin cancer, casualties, phlegm, omija, acidophilus and ogapi is lower than the lower limit, wrinkles may not be improved and skin elasticity may not be enhanced. Can occur.

The brown rice is preferably brown rice, which is mixed with the bagasse to improve skin wrinkles and improve skin elasticity during fermentation. The brown rice is mixed with 5 to 10 parts by weight, preferably 6 to 8 parts by weight, based on 100 parts by weight of white soda. When the content of the brown rice is less than the lower limit, excellent wrinkles are not improved and skin elasticity may not be enhanced, and when the content is above the upper limit value, resources can be wasted because it can not induce more excellent effects.

The yeast is a traditional fermentation agent of Korea, and the commonly used yeast is used to ferment other naturally occurring substances by mixing with useful microorganisms to improve skin wrinkles and skin elasticity, as well as to produce female hormones, Help. If none of the yeast and useful microorganisms are used, the skin wrinkles and skin elasticity may not be improved but may be lowered, and there is no effect on female hormones.

Nuruk is mixed in an amount of 5 to 10 parts by weight, preferably 6 to 8 parts by weight, based on 100 parts by weight of white bagasse. If the content of the yeast is below the lower limit, the fermentation may not be performed. If the content of the yeast exceeds the upper limit, the yeast is used more than necessary and resources are wasted.

The above-mentioned starch is used for fermentation, and specific examples thereof include cornstarch, bellflower, cornstarch and black garlic, preferably cornstarch.

The content of tonic acid is 100 to 300 parts by weight, preferably 150 to 200 parts by weight, based on 100 parts by weight of white seaweed. Fermentation may not be carried out when the content of oranges is less than the above lower limit, and in the case of exceeding the upper limit, waste more than necessary is used and resources are wasted.

The beneficial microorganism (EM) is mixed with yeast to ferment naturally occurring substances, thereby improving skin wrinkles and improving skin elasticity.

The content of the useful microorganism is 100 to 300 parts by weight, preferably 100 to 150 parts by weight based on 100 parts by weight of the bagasse. When the content of the useful microorganism is less than the lower limit, skin wrinkles and skin elasticity may not be enhanced. If the content of the microorganisms is above the upper limit value, unnecessary useful microorganisms are used and resources are wasted.

The mineral-enriched water is obtained by concentrating deep-sea water having a hardness of 300 mg / L to a hardness of 230,000 to 290,000 mg / L. The hardness is an index indicating the content of calcium carbonate in the water, which corresponds to the sum of the contents of calcium ions and magnesium ions in water. The higher the hardness, the larger the amount of minerals contained. When the hardness of the mineral-concentrated water is less than the lower limit, minerals such as calcium and magnesium can not be supplied to the skin. If the hardness of the mineral-concentrated water is above the upper limit, skin troubles may occur.

The content of the mineral-enriched water is 20 to 100 parts by weight, preferably 30 to 50 parts by weight, based on 100 parts by weight of the bagasse. When the content of the mineral-concentrated water is less than the lower limit value, excellent skin wrinkles and skin elasticity can not be expected. If the content is above the upper limit, skin trouble may occur.

In the present invention, it is necessary to first mix a mineral-concentrated water having a high hardness and natural-derived materials to coat or impregnate a large amount of concentrated minerals on the surface of natural-derived materials, and then ferment the marine deep- And minerals can improve their efficacy by fermentation. In this case, when the deep sea water and the yellow clay are added together with the mineral concentrated water later, it is not expected to improve the skin wrinkles and skin elasticity.

The content of the deep seawater is 1500 to 2000 parts by weight, preferably 1600 to 1700 parts by weight, based on 100 parts by weight of white seaweed. If the content of the deep sea water is out of the above range, it may interfere with fermentation.

The above-mentioned yellow clay is a supernatant after standing for 20 to 25 hours in a mixture of yellow soil and water, and contains various minerals such as potassium, sodium and iron, which are contained in mineral concentrated water and deep seawater, .

The content of the yellowing agent is 1500 to 2000 parts by weight, preferably 1600 to 1700 parts by weight, based on 100 parts by weight of the bagasse. When the content of the yellow clay is less than the lower limit, various minerals can not be supplied to the skin. If the content is above the upper limit, the yellow clay is used more than necessary and resources are wasted.

The mixture mixed in the step (A) is firstly fermented at 20 to 50 ° C, preferably 25 to 30 ° C for 12 to 20 days, preferably 13 to 14 days. During the first fermentation, the fermentation vessel is turned over once a day to allow the fermentation to be performed well. When the fermentation temperature and time are out of the above range, fermentation may not be performed well, and in particular, if the fermentation temperature is higher than the upper limit value, skin trouble may be caused.

Next, in the step (B), air is injected under the same temperature as that of the primary fermentation for secondary fermentation.

Air is injected for 12 to 20 days, preferably 13 to 14 days at the same temperature as the primary fermentation, thereby performing secondary fermentation while air bubbles are formed in the fermentation product.

When fermentation is carried out without air injection, secondary fermentation is not smoothly performed and, in some cases, toxicity may be induced.

In the present invention, step (C) is performed separately from steps (A) and (B).

On the other hand, in the step (C), Hwang, Ki - Young At least one selected from the group consisting of green tea, green tea, green tea, pine nut and walnut; At least one selected from the group consisting of licorice and coriander; Mineral concentrated water; And deep sea water are mixed and subjected to hot water extraction.

It is used together with the fermented product prepared in the step (B) to greatly enhance skin wrinkles and skin elasticity, and to produce female hormones, Hwanggi is used.

The content of the sulfur group is 100 to 300 parts by weight, preferably 120 to 150 parts by weight based on 100 parts by weight of the white soda. When the content of the hwanggi is less than the lower limit, the skin wrinkles and the skin elasticity are not improved and the female hormone is not produced. If the content is above the upper limit value, the skin wrinkles may occur and the elasticity of the skin may be lowered.

In addition, in order to further improve the skin wrinkles and skin elasticity, it is mixed with the fermented product prepared in step (B) to further improve skin wrinkles and skin elasticity. Pine nut, and walnut, preferably at least one of the above 14 species.

The content of at least one selected from the group consisting of green tea, green tea, green tea, green tea, green tea, green tea, korean tea, kochujang, Wealth. If the content is below the lower limit, skin wrinkles and skin elasticity may not be enhanced. If the content is above the upper limit, skin troubles and toxicity may be caused.

Also, in order to produce female hormone by mixing with the fermented product prepared in step (B), one or more selected from the group consisting of licorice and coriander, preferably two of them, are used together.

The content of at least one selected from the group consisting of licorice and coriander is 100 to 400 parts by weight with respect to 100 parts by weight of the bagasse. Licorice, and goji, the female hormone can not be produced. If the upper limit is exceeded, the female hormone may be weakly produced.

The content of the mineral-concentrated water is 20 to 50 parts by weight, preferably 25 to 30 parts by weight, based on 100 parts by weight of the bagasse. When the content of mineral-concentrated water is less than the lower limit value, skin wrinkles and skin elasticity are not enhanced, and when the content is above the upper limit value, no further effect is promoted.

The deep seawater is used for hot water extraction and is preferably used in an amount of 1000 to 20,000 parts by weight per 100 parts by weight of the bagasse.

The hot-water extract in step (C) is obtained by extracting the mixture at 70 to 90 ° C. for 100 to 130 hours, preferably 120 to 130 hours. If the extraction temperature and time are out of the above range, the active substances may not be extracted.

Next, in step (D), the secondary fermentation product fermented in the step (B) is filtered and separated into a fermentation broth and a residue.

The secondary fermented product is separated by filtration into a fermentation broth and a residue.

Next, in the step (E), the residue separated in the step (D) is extracted with hot water.

The residue separated in the step (D) and water are mixed at a weight ratio of 1: 1 to 3 and extracted with hot water at 70 to 90 ° C for 10 to 30 hours, preferably 15 to 20 hours to obtain an extract.

When the extraction temperature and the extraction time are out of the above range, the effective substance contained in the residue can not be extracted.

Next, in step (F), the fermentation broth separated in step (D), the residue extract extracted in step (E), and the extract extracted in step (C) are mixed.

The fermentation broth separated in step (D), the residue extract extracted in step (E), and the extract extracted in step (C) are mixed at a weight ratio of 1: 0.2-0.8: 0.5-3.0, 0.7: 1.0-1.5. When the content of the residue extract extracted at step (E) and the extract extracted at step (C) are lower than the lower limit value based on the fermentation broth separated in step (D), the skin wrinkles and skin elasticity are not improved and female hormone If it exceeds the upper limit value, it is toxic and skin trouble may occur.

If any of the fermentation broth separated in step (D), the residue extract extracted in step (E), and the extract extracted in step (C) is omitted, the skin wrinkles and skin elasticity can not be enhanced and female hormones can not be produced.

The naturally-occurring materials used in the present invention should be prepared according to the manufacturing method of the present invention, so that complementary pharmacological effects are exerted to improve skin wrinkles, promote skin elasticity, and produce female hormones.

The cosmetic composition for skin wrinkle improvement or skin elasticity enhancement of the present invention may be blended with cosmetic composition for skin wrinkle improvement or skin elasticity enhancement prepared according to the above production method, Examples of such a compounding component include a preservative, a humectant, an emollient, a surfactant, an organic and inorganic pigment, an organic powder, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an alcohol, a pigment, Limiting agents, and purified water.

The cosmetic composition for improving skin wrinkles or improving skin elasticity may be prepared in the form of an emulsified formulation and a solubilized formulation commonly used in the art, and examples thereof include a solution, an external ointment, a cream, a foam, a nutritional lotion, Sunscreen creams, sun oil, suspensions, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations , Emulsion foundation, wax foundation, patch, spray, and the like. In particular, the cosmetic composition for improving skin wrinkles or skin elasticity according to the present invention is preferable for cream or for eating cosmetics.

The present invention also provides a method for producing a food composition for improving skin wrinkles or improving skin elasticity.

Foods containing the food composition of the present invention include, for example, various foods, beverages, gums, tea, vitamin complex, and the like, but are not limited thereto.

The food composition for improving skin wrinkles or skin elasticity of the present invention includes forms such as tablets, capsules, pills, and liquids.

The beverage composition for improving skin wrinkles or improving skin elasticity of the present invention is not particularly limited to other components except for containing the food composition as an essential ingredient at the indicated ratio, and may be added with various flavors or natural carbohydrates As a component. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavors (tau martin, stevia extracts (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used as flavors other than those described above The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the food composition of the present invention.

In addition to the above, the food composition of the present invention can be used as a flavoring agent such as a variety of nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, coloring agents and thickening agents (cheese, chocolate etc.), pectic acid and its salts, Salts thereof, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like.

In addition, the food composition of the present invention may contain natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not so critical, but is generally selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the food composition of the present invention.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention. Such variations and modifications are intended to be within the scope of the appended claims.

Preparation Example 1. Preparation of the compound of step (A)

83 parts by weight of ginseng, 83 parts by weight of ginseng, 83 parts by weight of casualties, 83 parts by weight of casualties, 83 parts by weight of gumigae, 83 parts by weight of omija, 83 parts by weight of acid, and 83 parts by weight of ogapi were mixed with 6.6 parts by weight of rice brown rice, And 166 parts by weight of cornstarch, and then 33 parts by weight of mineral concentrated water and 100 parts by weight of useful microorganisms were added and mixed. Then, 1,600 parts by weight of deep sea water and 1,600 parts by weight of yellow clay were added.

Preparation Example 2. Preparation of the compound of step (C)

100 weight parts of white mud, 125 weight parts of sulfuric acid, 125 weight parts of green tea, 125 weight parts of cornstalks, 125 weight parts of curds, 125 weight parts of raw paper, 125 weight parts of kills, 125 weight parts of Seokchangpo, 125 parts by weight of Hwangjeol, 125 parts by weight of Korean red ginseng, 125 parts by weight of red ginseng, 125 parts by weight of chaga mushroom, 125 parts by weight of pine nuts and 125 parts by weight of walnuts were mixed with 100 parts by weight of licorice and 100 parts by weight of ginger, And 10000 parts by weight of deep sea water were added and mixed, followed by extraction at 80 DEG C for 120 hours to obtain a hot-water extract.

Example 1.

The compound of Preparation Example 1 was subjected to primary fermentation (inversion of the fermentation vessel once a day) at 30 ° C. for 14 days, then air was injected at the same temperature for 14 days to perform secondary fermentation Next, the secondary fermentation product was filtered and separated into a fermentation broth and a residue.

The residue and water were mixed at a weight ratio of 1: 2 and extracted at 80 DEG C for 20 hours to obtain a residue extract.

The filtered fermentation broth, the residue extract and the hot water extract of Preparation Example 2 were mixed at a weight ratio of 1: 0.6: 1.5 to prepare a cosmetic composition.

Comparative Example 1. Fermentation Omission

The compound of Preparation Example 1 and the hot-water extract of Preparation Example 2 were mixed at a weight ratio of 1: 2.1 to prepare a cosmetic composition.

Comparative Example 2. Omission of residue extract

The same procedure as in Example 1 was carried out except that the filtered fermentation broth without using the residue extract and the hot water extract of Preparation Example 2 were mixed at a weight ratio of 1: 2.1 to prepare a cosmetic composition.

Comparative Example 3. Manufacturing example 2 of Heat number Omit extract

The same procedure as in Example 1 was conducted except that the filtered fermentation broth and the residue extract were mixed at a weight ratio of 1: 2.1 to prepare a cosmetic composition.

< Test Example >

Test Example 1. Cytotoxicity test

Cytotoxicity was measured according to the concentration of the cosmetic composition prepared in Examples and Comparative Examples.

Cytotoxicity was measured by the method of Rochem et al. In order to carry out the test in the safety concentration range of the sample. Cells (2 × 10 ⁵ cells / well) (RAW 264.7) were seeded in 24-well plates and cultured for 24 hours. After that, the medium was removed and 1 mL of the medium containing 3% of the serum in which the extract was dissolved was dispensed and cultivation was carried out for 24 hours. After 24 hours, 0.25 mL of XTT-PMS solution (1 mg XTT and 10 g PMS / mL of MEM without phenol red) was added to each well and incubated for another 2 hours. The degree of cytotoxicity was determined by measuring the absorbance of formazan at 450 nm using a microplate reader. The medium was MEM supplemented with 10% (v / v) fetal bovine serum (FBS), 0.5% (v / v) 50 g / mL streptomycin, 50 IU / mL penicillin, 0.125 g / mL fungizone, 3.024 g sodium bicarbonate And incubated at 37 ℃, 5% CO _2, 95% humidified air.

division Cell viability (%) 10 [mu] g / ml 25 [mu] g / ml 50 [mu] g / ml 100 [mu] g / ml Example 1 112.5 108.9 105.7 104.8 Comparative Example 1 56.3 51.1 42.8 33.2 Comparative Example 2 101.9 99.3 93.7 90.1 Comparative Example 3 103.1 101.5 97.2 88.3

As shown in Table 1, it was confirmed that the cosmetic composition prepared according to Example 1 of the present invention showed no toxicity in the interval of 10 to 100 占 퐂 / ml.

On the other hand, in Comparative Example 1, toxicity was observed in the entire section of 10 to 100 占 퐂 / ml, while in Comparative Examples 2 and 3, toxicity was observed in 25 占 퐂 / ml.

Test Example 2. Collagen Sum performance

The effect of wrinkle improvement or skin elasticity improvement can usually be measured as collagen biosynthesis performance, collagenase degradation inhibition ability and human clinical effect. The cosmetic composition prepared in Examples and Comparative Examples was added to a culture solution of human-derived fibroblasts to examine the promoting effect of collagen synthesis at the cellular level. The biosynthetic collagen was quantitated using a PICP EIA kit (Procollagen Type I CPeptide Enzyme Immunoassay KIT).

Human normal fibroblasts were cultured in DMEM medium at 37 ° C for 24 hours in a 5% CO ₂ incubator. The cosmetic composition and vitamin C (Vitamin C) were added to the human fibroblast culture medium and cultured for 1 day with the control (untreated). After 1 day, the amount of collagen biosynthesis was determined by the PICP EIA kit at 450 nm using a spectrophotometer. Calculation of collagen biosynthesis performance is shown in Table 2, measured by the relative increase in relative performance compared to the control (untreated).

division Increase in collagen synthesis (%) compared to control 10 [mu] g / ml 25 [mu] g / ml 50 [mu] g / ml 100 [mu] g / ml Vitamin C 13 28 29 18 Example 1 25 63 84 72 Comparative Example 1 5 10 9 4 Comparative Example 2 10 28 20 14 Comparative Example 3 12 29 25 20

As shown in Table 2 above, it was confirmed that the cosmetic composition prepared according to Example 1 of the present invention was superior in collagen synthesis to vitamin C and Comparative Examples 1 to 3. Particularly, it was confirmed that collagen synthesis ability was the most excellent at 50 占 퐂 / ml of Example 1.

This means that the cosmetic composition of Example 1 exhibits excellent collagen combining performance against human-derived fibroblasts.

Test Example 3. Effect of wrinkle improvement

The wrinkle-reducing effect was measured using the experimental group treated with the cosmetic composition prepared according to Examples and Comparative Examples and the control group not treated. Wrinkle-improving effect The animal test was performed using a hairless mouse that was 6 weeks old. As a sample, the cosmetic composition was applied to 1,3-butylene glycol at 50 μg / ml. Hairless mice were irradiated with MED (minimal crythema dosis) using a solar simulator for 1 week to 3 days for 10 weeks to form wrinkles. Then, 1,3-butylene glycol (Butylene glycol) and 50 ㎍ / ㎖ of cosmetic composition were treated with 0.1 ml / cm ² twice a day for 6 weeks.

The degree of wrinkle improvement was determined by naked eyes through naked eyes and photographs, and then the sample treatment group and the control group were compared according to the following criteria compared with before treatment. The population of each group is 10.

division No improvement Slight improvement A significant improvement Control group 10 0 0 Example 1 0 2 8 Comparative Example 1 10 0 0 Comparative Example 2 8 2 0 Comparative Example 3 6 4 0

As shown in Table 3 above, it was confirmed that the cosmetic composition prepared according to Example 1 of the present invention exhibited excellent wrinkle-reducing effect as compared with the control group and Comparative Examples 1 to 3.

Test Example 4. Wrinkle improvement and skin elasticity Clinical effect

A sample containing the present invention (Example 1, Comparative Example 1-3) was applied to 1,3-butylene glycol at a concentration of 50 占 퐂 / ml to 100 subjects (25 subjects per sample) After that, it was applied twice a day in the morning and in the evening for 3 months. The degree of wrinkle improvement and skin elasticity after use was judged according to the following criteria.

division No improvement Slight improvement A significant improvement Example 1 0 5 20 Comparative Example 1 25 0 0 Comparative Example 2 23 2 0 Comparative Example 3 21 4 0

As shown in Table 4, the cosmetic composition prepared according to Example 1 of the present invention exhibited excellent wrinkle-improving effect and confirmed that skin elasticity was improved.

On the other hand, Comparative Example 1 showed no improvement in wrinkle and skin elasticity, and Comparative Examples 2 and 3 also showed lower wrinkle and skin elasticity.

Test Example 5. Estrogen content measurement

Six-week old female Sprague Dawley (200 g) experimental animals were added with the food composition prepared in Examples and Comparative Examples to a concentration of 50 μg / ml in the feed, and the feeds were taken for 28 days, respectively. Control groups were fed diets without added food composition.

The ovaries were isolated from each group, and the animals were sacrificed for 16 hours before the autopsy. The anesthetized animals were anesthetized and the abdominal cavity was opened. Blood was collected from the abdominal vein and centrifuged at 2500 rpm for 15 minutes After isolating the serum, the following results were obtained by RIA method.

division Control group Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Estrogen
(pg / ml) 58 75 58 60 62

As shown in Table 5, it was confirmed that the composition prepared according to Example 1 of the present invention produced a large amount of estrogen.

On the other hand, in Comparative Examples 1 to 3, estrogen was not produced because the estrogen content of the control group was similar.

Since the composition of Example 1 produces a large amount of estrogen, female hormone can be supplied.

<Formulation example>

Hereinafter, formulation examples of the composition containing the extract of the present invention will be described, but the present invention is not intended to be limited thereto but is specifically described.

Formulation Example 1. Preparation of cosmetic product of emulsified formulation

Cosmetics of emulsified form such as nutrient lotion, cream, essence, and cosmetics of solubilized form such as softened longevity were prepared.

Emulsifier type cosmetics were prepared in the compositions shown in Table 6 below. The production method is as follows.

1) A mixture of raw materials 1 to 9 was heated to 65 to 70 占 폚.

2) The starting material of 10 was added to the mixture of step 1).

3) The mixture of raw materials 11 to 13 was completely dissolved by heating to 65 to 70 占 폚.

4) While the above step 3) was carried out, the mixture of step 2) was gradually added and emulsified at 8,000 rpm for 2 to 3 minutes.

5) The raw material of 14 was dissolved in a small amount of water and then added to the mixture of step 4) and further emulsified for 2 minutes.

6) The raw materials of 15 to 17 were each weighed, and then put into the mixture of step 5) and further emulsified for 30 seconds.

7) The mixture of step 6) was degassed after emulsification and cooled to 25-35 占 폚 to prepare an emulsifier-type cosmetic.

Furtherance Emulsifier type 1 Emulsifier type 2 Emulsifier type 3 One Stearic acid 0.3 0.3 0.3 2 Stearyl alcohol 0.2 0.2 0.2 3 Glyceryl monostearate 1.2 1.2 1.2 4 Wax 0.4 0.4 0.4 5 Polyoxyethylene sorbitan
Monolauric acid ester 2.2 2.2 2.2 6 Methyl paraoxybenzoate 0.1 0.1 0.1 7 P-hydroxybenzoic acid profile 0.05 0.05 0.05 8 Cetyl ethyl hexanoate 5 5 5 9 Triglyceride 2 2 2 10 Cyclomethicone 3 3 3 11 Distilled water ~ 100 ~ 100 ~ 100 12 Concentrated glycerin 5 5 5 13 Triethanolamine 0.15 0.15 0.15 14 Polyacrylic acid polymer 0.12 0.12 0.12 15 Pigment 0.0001 0.0001 0.0001 16 incense 0.10 0.10 0.10 17 The cosmetic composition of Example 1 0.0001 One 10

Formulation example 2. Solubilization Manufacture of Cosmetics for Formulation

Cosmetic products of the solubilized formulations were prepared with the compositions shown in Table 7 below. The production method is as follows.

1) 2 to 6 raw materials were put into 1 raw material (purified water) and dissolved using a mixer.

2) Raw materials 8 to 11 were completely dissolved in 7 raw materials (alcohol).

3) The mixture of step 2) was slowly solubilized by adding it to the mixture of step 1).

Furtherance Solubilization Formulation 1 Solubilization Formulation 2 Solubilization Formulation 3 One Purified water ~ 100 ~ 100 ~ 100 2 Concentrated glycerin 3 3 3 3 1,3-butylene glycol 2 2 2 4 EDTA-2Na 0.01 0.01 0.01 5 Pigment 0.0001 0.0002 0.0002 6 The cosmetic composition of Example 1 0.1 5 5 7 Alcohol (95%) 8 8 8 8 Methyl paraoxybenzoate 0.1 0.1 0.1 9 Polyoxyethylene
Hydro genide ester 0.3 0.3 0.3 10 incense 0.15 0.15 0.15 11 Cyclomethicone - - 2

Formulation Example 3. Preparation of Eating Cosmetics

Composition of Example 1 19 kg

Betaclaran 5 kg

Collagen 0.6 kg

Fructose 4 kg

Alpha tocopherol 0.3 kg

Pectin 0.5 kg

Citric acid 0.3 kg

Purified water 50 L

Number of conversions 100 L

Formulation Example 4. Preparation of functional beverage

The composition of Example 1 1,000 mg

Citric acid 1,000 mg

100 g of oligosaccharide

Plum concentrate 2 g

Taurine 1 g

Purified water was added to the flask to obtain a total of 900 mL

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was stirred and heated at 85 DEG C for about 1 hour. The solution thus prepared was filtered and sterilized in a sterilized 2 L container, It is used in the production of the functional beverage composition of the invention.

Although the composition ratio is a mixture of the components suitable for the preferred beverage as a preferred embodiment, the blending ratio may be arbitrarily varied according to the regional and national preferences such as the demand level, the demanding country, and the intended use.

Claims

(A) useful microorganisms which are white liquor, bokbunja, earthworm, casualty, gugija, omija, liquorice, ogapi, brown rice, yeast, zucchini, EM solution; Mixing the mineral water with mineral water, adding the deep sea water and yellow clay water to the first fermentation;
(B) injecting air under the same temperature as that of the primary fermentation to perform secondary fermentation;
(C) Mixed with deep seawater, concentrated water of mineral water and deep sea water, pine nut, hwanggi, jaejong yong, paradigm, mulberry, raw paper, killing, Seokchangpo, Seokgiwang, Baekbyeong, Hwangjeong, broiler, red ginseng, Extracting the hot water;
(D) separating the fermented product fermented in step (B) into a fermentation broth and a residue;
(E) hot water extraction of the residue separated in step (D); And
(F) mixing the fermentation broth separated in the step (D), the extract extracted in the step (E) and the extract extracted in the step (C). A method for producing a cosmetic composition for improving skin wrinkles or skin elasticity using a derived material.

[7] The method of claim 1, wherein 50 to 600 parts by weight of the group consisting of bokbunja, earthworm, casualty, gugija, omija, liquorice, and ogap is 100 parts by weight of bagasse in step (A) 5 to 10 parts by weight of brown rice; 5 to 10 parts by weight of yeast; 100 to 300 parts by weight of crude oil; 100 to 300 parts by weight of useful microorganisms; 20 to 100 parts by weight of mineral concentrated water; 1500 to 2000 parts by weight of deep ocean water; And 1500 to 2000 parts by weight of a yellowing agent. The method for manufacturing a cosmetic composition for improving skin wrinkles or skin elasticity using a naturally-derived material comprising white backbone.

The method according to claim 1, wherein the primary fermentation in step (A) is performed at 20 to 50 ° C for 12 to 20 days. The method for improving skin wrinkles or skin elasticity using a natural- Gt;

The method according to claim 1, wherein the secondary fermentation is performed for 12 to 20 days in the step (B). The method for producing a cosmetic composition for improving skin wrinkles or skin elasticity using a naturally- .

The method according to claim 1, wherein, in step (C), 100-300 parts by weight of a sulfur-containing compound is added to 100 parts by weight of white algae; 100 to 3500 parts by weight of a group consisting of green tea, green tea, green tea, green tea, kelp, kochujang, seokjanghwang, white bongryeong, hwangjeong, broiler, red ginseng, pine nut and walnut; 100-400 parts by weight of a group consisting of licorice and coriander; 20 to 50 parts by weight of mineral concentrated water; And 1000 to 20,000 parts by weight of deep sea water. The method for manufacturing a cosmetic composition for improving skin wrinkles or skin elasticity using a natural-derived material containing white sewage.

The method of claim 1, wherein the hot-water extract in step (C) is extracted from the mixture at 70 to 90 ° C for 100 to 130 hours to improve skin wrinkles or skin elasticity using natural- Wherein the cosmetic composition is a cosmetic composition.

The method according to claim 1, wherein the hot water extract in step (E) is obtained by mixing the residue and water at a weight ratio of 1: 1 to 3 and extracted at 70 to 90 ° C for 10 to 30 hours. A method for producing a cosmetic composition for improving skin wrinkles or skin elasticity using a naturally occurring substance.

2. The method according to claim 1, wherein the fermentation broth separated in step (D), the extract extracted in step (E), and the extract extracted in step (C) Wherein the composition is mixed at a weight ratio of about 3.0 to about 3.0. The method for manufacturing a cosmetic composition for improving skin wrinkles or skin elasticity using a naturally-occurring material containing white backbone.

9. A cosmetic composition for improving skin wrinkles or skin elasticity, which is prepared according to any one of claims 1 to 8.

(A) useful microorganisms which are white liquor, bokbunja, earthworm, casualty, gugija, omija, liquorice, ogapi, brown rice, yeast, zucchini, EM solution; Mixing the mineral water with mineral water, adding the deep sea water and yellow clay water to the first fermentation;
(B) injecting air under the same temperature as that of the primary fermentation to perform secondary fermentation;
(C) Mixed with deep seawater, concentrated water of mineral water and deep sea water, pine nut, hwanggi, jaejong yong, paradigm, mulberry, raw paper, killing, Seokchangpo, Seokgiwang, Baekbyeong, Hwangjeong, broiler, red ginseng, Extracting the hot water;
(D) separating the fermented product fermented in step (B) into a fermentation broth and a residue;
(E) hot water extraction of the residue separated in step (D); And
(F) mixing the fermentation broth separated in the step (D), the extract extracted in the step (E) and the extract extracted in the step (C). A method for producing a food composition for improving skin wrinkles or skin elasticity using a derived material.