KR20100101257A - Compositions for uv protection, anti-inflammatory, antioxidation and antiaging - Google Patents

Abstract

PURPOSE: A composition containing Galla Phois fermentation for blocking UV ray is provided to use as an anti-inflammation and anti-oxidation composition and to variously use in cosmetic and medical field. CONSTITUTION: A composition for blocking UV ray contains Galla Phois fermentation as an active ingredient. A composition for anti-inflammation and antioxidation contains Galla Phois fermentation as an active ingredient. The Galla Phois fermentation is obtained by fermenting Galla Phois using lactobacillus or yeast. The lactobacillus is Lactobacillus sakei, Lactobacillus brevis, Lactobacillus plantarum, Lactococcus lactis or Bacillus coagulance. The yeast is Saccharomyces cerevisiae.

Description

Composition, UV protection, anti-inflammatory, antioxidation and antiaging

The present invention relates to a composition containing a gall bladder fermentation having excellent UV blocking activity.

Herbal medicines have been used for a long time in the cosmetics industry, and recently, herbal cosmetics have become the mainstream of cosmetic products. Herbal cosmetics have been used for hundreds of years and have been proven in terms of safety and effectiveness, so they can be easily recognized and used by consumers. In addition, the effect is also inferior to synthetic materials. The herbal medicine contains various bioactive substances, and for example, has various effects such as anti-aging, anticancer, anti-inflammatory, and immune function improvement.

In particular, the inventors of the present invention, while studying various physiological activities on the gall, found that the gall fermented product had superior efficacy compared to a simple extract and completed the present invention.

It is an object of one embodiment of the present invention to provide a gall bladder fermentate-containing composition having excellent UV blocking effect.

It is an object of another embodiment of the present invention to provide a gall bladder-containing composition having anti-inflammatory efficacy.

It is an object of another embodiment of the present invention to provide a gall bladder fermentation composition having an antioxidant effect.

It is an object of another embodiment of the present invention to provide a composition containing a gall bladder fermentation having an anti-aging effect.

The composition containing the gall bladder fermentation product according to the present invention as an active ingredient is characterized in that the composition for sunscreen. In addition, the composition is characterized in that the anti-inflammatory, antioxidant and anti-aging composition.

The gall bladder-containing composition according to the present invention exhibits excellent sunscreen effect, anti-inflammatory, antioxidant and anti-aging effects are recognized, and can be used in various fields such as beauty and medicine.

The composition according to the embodiment of the present invention is characterized by containing a fermented gall (Galla Phois, Malaphis chinensis) as an active ingredient. The composition containing the gall bladder fermentation is excellent in the sunscreen effect, anti-inflammatory, antioxidant and anti-aging efficacy is recognized.

In the present invention, the "galloper" is a generic term for insect nodules that are made by parasitic nematodes of cicada aphids parasitic on the leaves of Rhodes of Lacaceae.

In one embodiment, the red tree is a sumacaceae, and when aphids are parasitic on the wings of petioles, bumps, such as large balloon bags, pop out to form an insect worm called a gall bladder. In addition, red wood is also used as a raw material for making natural dyes and inks dyeing cloth, and the effect of treating acute, chronic colitis, prolonged cough, diarrhea has been known since ancient times. Rhododendrons are named red trees because they have beautiful red leaves in autumn. Chinese characters are called salt splint (부 木) because the fruit is salty and salty. When the fruit is ripe, the white powder is very sour and salty. In addition, the leaves have a bumpy bag that looks bumpy, and there are a lot of small bugs in it. The only salt in the tree is red wood, and those who lived in the mountains far away from the sea rubbed the red fruit into the water and used it to make tofu instead of salt or tofu.

In addition, white gin from red leaves and bark is good for treating burns, skin diseases, and small wounds. In particular, white gin from red wood can be applied to burns without leaving scars. Rhododendron bark and leaves are particularly effective against acute or chronic enteritis. Finely chop the leaves until it becomes syrupy and eat it with a strong sweetening.

In some cases, the gall bladder is a rhododendron worm house, also known as anastomosis, baekchungchang (白 蟲 倉). In September-October, pick a Rhododendron worm and steam it to dry. Taste is bitter and sour. Menopause, stomach, and colonoscopy, intestinal (腸) to stop diarrhea, stop bleeding and sweat, sore boils and cough is effective to stop.

In one embodiment, the main component of the gall bladder is tannin and contains about 50-60% of the gall bladder. Tannins are also used to prevent alkaloid poisoning because they sink alkaloids into sediments. The gall bladder has been used to treat dysentery, prolapse, cold, cold, and oil wells because of its strong convergence, which stops chronic seawater caused by weak lung function. Hemostatic action stops fecal bleeding, nosebleeds, uterine bleeding and is also effective for traumatic bleeding. It is also effective for boils, dermatitis and itching. In pharmacological action, the gallotannin component of the gall bladder has a convergent action, and when it comes into contact with the skin mucosa, tissue proteins coagulate and show a convergent effect. It has antibiotic effect on dysentery, Pseudomonas aeruginosa, etc. and has antibiotic action to reduce sperm. Hepatoprotective and antioxidant activity is shown.

An exemplary process of creating a gallant is as follows. The winged worm, which drills through the worm house in late September, gives birth to worms in the midhost Minum vesicatum (M. trichomane). The larvae suck on the juices of the parasites and grow to make cocoons, which are worms with white lead, and winter. In the spring of the following year, it becomes a pupa and emerges from the pupa in late April to become a winged worm. The female worms lay off wingless male larvae on the branches of the red tree after mating. Wingless worms migrate to young leaves to grow parasites. In one worm house, worms grow to an average of 400 by early October. Then, through a fake chrysalis, it grows into a winged worm and breaks through the hole. This gives birth to larvae in the middle host. If you plant a lot of red trees and pick a worm house, you can make a lot of herbs in the shaded and humid place where a lot of phytopods grow. As a result of the histological study of the process of the formation of worms, it was seen as abnormal proliferation of plant cells by external stimuli, also called plants or plant tumors. Research on plant lumps has been very helpful for anticancer drug research.

In one embodiment, the gall bladder fermentation may be fermented by adding either or both of lactic acid bacteria or yeast. Examples of the lactic acid bacterium include, but are not particularly limited to, Lactobacillus sakei , Lactobacillus brevis, Lactobacillus plantarum , Lactococcus lactis , and the like. Bacillus coagulance ( Bacillus coagulance ) may be used any one selected from the group consisting of or a mixed strain thereof. The yeast is not particularly limited, but Saccharomyces cerevisiae can be used, for example.

In one embodiment, the gall bladder fermentation-containing composition may be a composition for sunscreen. For example, the gall bladder extract has an effect of strongly absorbing ultraviolet rays. In particular, it was confirmed through various experiments that the fermented fermented product mixed with fermentation by adding lactic acid bacteria and yeasts to the gall bladder, UV absorption ability increased by about 30% compared to the gall bladder extract. Therefore, the gall bladder fermented product-containing composition has the effect of preventing skin damage by ultraviolet rays and improving skin condition.

In another embodiment, the gall bladder fermentation-containing composition may be an anti-inflammatory composition. The composition is recognized for the effect of inhibiting and alleviating the inflammatory response. For example, there is an effect of alleviating and suppressing inflammation caused by ultraviolet light.

In one embodiment, the gall bladder fermentation-containing composition may be an antioxidant composition. Fermented products fermented by using lactic acid bacteria, yeast, or mixed strains of lactic acid bacteria and yeast will significantly increase the antioxidant activity. For example, compared to gall bladder extract, gall bladder fermentation increases antioxidant activity by about 30%.

In one embodiment, the gall bladder fermentation-containing composition may be an anti-aging composition. Gall bladder fermentation has the effect of inhibiting aging by promoting collagen synthesis. For example, it was confirmed through experiments that gall bladder fermentation has an effect of inhibiting collagen reduction by ultraviolet rays.

In addition, the gall bladder fermented product-containing composition is recognized to suppress and improve wrinkles of the skin and protect skin keratinocytes from reactive oxygen species.

In one embodiment, the content of the gall bladder fermentation, based on the total weight of the composition may be 0.001% to 98% by weight, specifically 0.05% to 95% by weight, more specifically 0.01 to 85% by weight. have. The content of such gall bladder fermentation is to improve the skin condition improvement effect according to the addition of gall bladder fermentation.

In one embodiment, the present invention provides a cosmetic composition containing the gall bladder fermentation. The cosmetic composition may be used for effects such as skin damage prevention and improvement, anti-aging and wrinkle improvement by ultraviolet rays. The cosmetic composition is not particularly limited and may be formulated in various forms such as creams, lotions, skins, essences, emulsion soaps, foam cleansing, packs, shampoos, rinses, body washes, and the like.

In another embodiment, the present invention provides a pharmaceutical composition containing the gall bladder fermentation. The pharmaceutical composition can be used for effects such as skin protection, anti-aging, skin irritation, wrinkle improvement and skin protection from reactive oxygen species from ultraviolet rays.

When the composition according to the present invention is applied to a pharmaceutical product, it is formulated as an oral or parenteral dosage form in solid, semi-solid or liquid form by adding a commercially available inorganic or organic carrier with the copper or its extract as an active ingredient. can do.

Examples of the preparation for oral administration include tablets, pills, granules, soft and hard capsules, powders, fine granules, powders, emulsions, syrups, pellets, and the like. Can be mentioned. In addition, preparations for parenteral administration include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. In order to formulate the active ingredient of the present invention, it can be easily formulated according to the conventional method, and surfactants, excipients, coloring agents, spices, preservatives, stabilizers, buffers, suspending agents, and other commonly used auxiliaries can be suitably used.

The pharmaceutical composition according to the present invention may be administered orally, parenteral, rectal, topical, transdermal, intravenous, intramuscular, intraperitoneal, subcutaneous.

In addition, the dosage of the active ingredient will vary depending on the age, sex and weight of the subject to be treated, the specific disease or pathology to be treated, the severity of the disease or pathology, the route of administration and the judgment of the prescriber. Dosage determination based on these factors is within the level of those skilled in the art, and generally the dosage ranges from 0.001 mg / kg / day to approximately 2000 mg / kg / day. More preferred dosages are from 0.5 mg / kg / day to 2.5 mg / kg / day.

The present invention also provides a functional food or food additive comprising the above gall bladder fermentation. It can be processed into fermented milk, cheese, yogurt, juice, probiotic and health supplements, including the composition comprising the gall bladder fermentation, it can be used in the form of various other food additives. Functional foods or food additives containing the gall bladder fermentation may exhibit a variety of efficacy, such as improvement of skin condition, inhibition of inflammation, antioxidant and anti-aging.

In one embodiment, the composition may contain other ingredients and the like that can give a synergistic effect to the main effect within a range that does not impair the main effect of the present invention. For example, it may further include additives such as perfumes, pigments, fungicides, antioxidants, preservatives, moisturizers, thickeners, inorganic salts, emulsifiers and synthetic polymer materials to improve physical properties. In addition, supplementary ingredients such as water soluble vitamins, oil soluble vitamins, polymer peptides, polymer polysaccharides and seaweed extract may be further included. The components may be appropriately selected and blended by those skilled in the art according to the formulation or purpose of use, and the amount of the additives may be selected within a range that does not impair the object and effect of the present invention. For example, the addition amount of the components may be in the range of 0.01 to 5% by weight, more specifically 0.01 to 3% by weight, based on the total weight of the composition.

In addition, the formulation of the composition according to the present invention may also be in various forms such as solution, emulsion, viscous mixture, tablet, powder, etc., which may be administered by various methods such as simple drinking, injection, spray or squeeze method. .

Hereinafter, the present invention will be described in more detail with reference to examples. However, the following examples and the like are intended to confirm the effects of the present invention by way of example, and the scope of the present invention is not limited thereto.

Example 1 Preparation of Mellitus Extract

The gall bladder dry was crushed and mixed with water to 10% by dry weight. Then extracted at 80 ° C. for 4 hours, then filtered and dried.

Example 2 Preparation of Fermented Product Using Mellitus Extract

By centrifugation at 5, 000 rpm of the gall bladder extract prepared in Example 1, insoluble components were removed and the water-soluble extract was separated. After sterilizing the isolated water-soluble extract for 15 minutes at 121 ℃, Lactobacillus sakei ( Lactobacillus sakei ) B2-16 (Accession No .; KFCC-11321) and Saccharomyces cerevisiae yeast ( Accession No .: KCCM 11201) was inoculated so that the initial bacterial count was 10 ⁷ cfu / g. After inoculation, the cells were left to culture at 30 ° C. for 3 days and then centrifuged to obtain a fermented supernatant.

Example 3 Preparation of Fermented Product Using Mellitus Extract

Lactobacillus plantarum ( Lactobacillus plantarum ) as a fermentation strain during fermentation A fermentation supernatant was obtained in the same manner as in Example 2, except that p23 (commercially available from Bioben Co., Ltd.) was used.

Example 4 Preparation of Fermented Products Using Mellitus Extract

The fermentation supernatant was prepared in the same manner as in Example 2, except that Lactococcus lactis A164 (Accession No .; KFCC-11047), which was a lactic acid bacterium, was used as a fermentation strain during fermentation of the gall bladder extract. Got it.

Experimental Example 1 Antioxidant Effect

After dissolving DPPH (diphenylpicrylhydrazyl) in ethanol to a concentration of 500 μM, the DPPH solution was diluted to prepare a light absorbance in the range of 0.8-0.9 at a wavelength of 517 nm. The DPPH solution was mixed with the fermented supernatant supernatant according to Example 2 at a ratio of 1: 1, and then reacted in a dark room for 20 minutes. By measuring the absorbance at a wavelength of 517 nm after the reaction, the antioxidant activity (Free radical scavenging activity) of the gall bladder fermentation sample was measured. The measurement results are shown in Table 1 and FIG. 1.

sample Concentrations capable of 50% scavenging of 500 μM DPPH Gall bladder extract 0.00066 100 Vitamin c 0.00075 88 gallnut
Lactic acid bacteria + yeast complex fermentation 0.00051 130

Referring to Table 1 and Figure 1, it can be seen that the antioxidant power of about 5% is increased compared to the fermented gallja fermentation.

In addition, it is shown in Figure 2 the results of measuring the antioxidant activity of the gall fermentation product is safe for 6 months at 4 ℃ dark conditions, room temperature light conditions, room temperature dark conditions, 45 ℃ dark conditions. Referring to Figure 2, it was confirmed that the antioxidant power is maintained relatively constant, unlike the initial.

Experimental Example 2 UV Absorption Effect

In order to evaluate the ultraviolet absorbing power of the five-fermented fermentation, the absorbance at 200-800 nm was measured from the ultraviolet region to the visible region using an absorbance measuring instrument. The measurement results are shown in FIG. 3.

Referring to Figure 3, it can be seen that the gall bladder extract and fermented product show a strong absorption in the medium-wave ultraviolet region (UV-B). In addition, it can be seen that the fermented product increases UVB absorption by about 30% compared to the extract. These results show about 1/3 of OMC (octylmethyl cinnamate), which is widely used in cosmetics (see Fig. 4), showing the potential as a natural UV absorber.

Experimental Example 3 Inhibition of Inflammatory Response

First, human keratinocytes were placed in DMEM (Dulbecco's Modified Eagle's Medium) medium containing penicillin (100 IU / mL), streptomycin (100 g / mL), and 10% FBS (fetal bovine serum). The cells were cultured in an incubator containing 5% carbon dioxide while maintaining 37 ° C. The obtained keratinocytes were dispensed into 6-well plates at 3 × 10 ⁵ per well and incubated for 24 hours under cell culture conditions. After culturing, the cells were irradiated with 25 mJ / Cm ² of ultraviolet rays, and then the embryonic fermentation supernatant according to Example 2 was added and further cultured for 24 hours. After culturing, the culture supernatant was recovered and the amount of inflammatory cytokine TNF-α was measured. The measurement results are shown in FIG. 5.

Referring to Figure 5, in the case of five-fermented fermented product decreases the secretion of the inflammatory cytokine TNF-α (Tumor necrosis factor α) is increased by the ultraviolet rays, the fermented product is superior to the case without the fermentation Able to know.

Experimental Example 4 Collagen Synthesis Promoting Effect (Anti-Aging Effect)

In order to confirm the anti-aging effect of fermented fermented gall bladder, we investigated the effect of promoting collagen synthesis in human fibroblasts.

First, normal human fibroblasts were charged with DMEM (Dulbecco's Modified Eagle's Medium) medium containing penicillin (100 IU / mL), streptomycin (100 g / mL), and 10% FBS (fetal bovine serum). The cells were cultured in an incubator containing 5% carbon dioxide while maintaining 37 ° C. The obtained fibroblasts were dispensed in 6-well plates at 3 × 10 ⁵ per well and incubated for 24 hours under cell culture conditions. After culturing, the cells were irradiated with 25 mJ / Cm ² of ultraviolet rays, and then the embryonic fermentation supernatant according to Example 2 was added and further cultured for 24 hours. After collecting and crushing the cells, the amount of collagen was measured after using a collagen collage kit (Sircol assay kit). The measured result is shown in FIG.

Referring to Figure 6, it can be seen that the gall bladder ferment inhibits the collagen decrease by ultraviolet irradiation.

Hereinafter, the present invention will be described in detail through the formulation examples of the composition, but the scope of the present invention is not limited thereto.

Preparation Example 1 Soap-Type Preparation

Extract of Example 1 5.00

Upkeep 75

Sodium hydroxide 5

Spices 10

Purified water level

(Unit: wt%)

Preparation Example 2 Lotion Type Formulations

Extract of Example 1 3.00

L-ascorbic acid-2-magnesium phosphate salt 1.00

Water Soluble Collagen (1% Aqueous Solution) 1.00

Sodium Citrate 0.10

Citric Acid 0.05

Licorice Extract 0.20

1,3-butylene glycol 3.00

Purified water level

(Unit: wt%)

Preparation Example 3 Cream

Extract of Example 1 1.00

Polyethylene Glycol Monostearate 2.00

Self-emulsifying glycerin monostearate 5.00

Cetyl Alcohol 4.00

Squalene 6.00

Tri2-ethylhexaneglyceryl 6.00

Sphingolipids 1.00

1.3-butylene glycol 7.00

Purified water level

(Unit: wt%)

Preparation Example 4 Packed Formulation

Extract of Example 1 5.00

Polyvinyl Alcohol 13.00

L-ascorbic acid-2-magnesium phosphate salt 1.00

Lauroylhydroxyproline 1.00

Water Soluble Collagen (1% Aqueous Solution) 2.00

1,3-butylene glycol 3.00

Ethanol 5.00

Purified water level

(Unit: wt%)

Preparation Example 5 Cosmetic Liquid Formulation

Extract 2.00 of Example 1

Hydroxyethylene Cellulose (2% Aqueous Solution) 12.00

Xanthan gum (2% aqueous solution) 2.00

1,3-butylene glycol 6.00

Dark Glycerin 4.00

Sodium Hyaluronate (1% aqueous solution) 5.00

Purified water level

1 is a graph showing the results of measuring the antioxidant activity (Free radical scavenging activity) of the gall bladder fermentation by measuring the absorbance at 517 nm wavelength;

Figure 2 is a graph showing the results of comparative measurements for 6 months the stability of the antioxidant activity of the gall bladder fermentation;

3 is a graph showing the results of comparing and measuring the ultraviolet absorption power of the gall bladder fermentation;

Figure 4 is a graph showing the results of comparing the ultraviolet absorption of the five-fermented fermentation in the medium wavelength ultraviolet region (UV-B);

5 is a graph showing the results of comparative measurements of the secretion amount of TNF-α in cells to which the embryonic fermentation was added;

Figure 6 is a graph showing the results of comparative measurement of the amount of collagen using a circol collagen analysis kit in the cells to which the gall bladder ferment was added.

Claims (10)

UV-blocking composition containing a galleng fermented product as an active ingredient. Anti-inflammatory composition containing the galleng fermented product as an active ingredient. Antioxidant composition containing a galleng fermented product as an active ingredient. Aging fermented product as an active ingredient, anti-aging composition for promoting collagen synthesis. The method according to any one of claims 1 to 4, The fermented fertilizer is a composition characterized in that the fermentation by adding any one or both of the lactic acid bacteria or yeast to the galleng extract. The method of claim 5, Lactobacillus sakei ( Lactobacillus sakei ), Lactobacillus brevis ( Lactobacillus brevis), Lactobacillus plantarum ( Lactobacillus plantarum ), Lactococcus lactis ( Lactococcus lactis ) and Bacillus coagulance ( Bacillus coagulance ) composition, characterized in that any one or two or more strains selected from the group consisting of. The method of claim 5, The yeast is a composition characterized in that Saccharomyces cerevisiae ( Saccharomyces cerevisiae ). The method of claim 5, The fermented fermented product is a composition characterized in that the fermentation by adding a mixed strain of lactic acid bacteria and yeast to the gall extract. The method of claim 8, The lactic acid bacteria is Lactobacillus sakei ( Lactobacillus sakei ), the yeast is a composition characterized in that Saccharomyces cerevisiae ( Saccharomyces cerevisiae ). The method according to any one of claims 1 to 4, The content of the gall bladder fermentation composition, characterized in that 0.05 to 95% by weight based on the total weight of the composition.

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