RU2485598C1 - Method of increasing non-specific body resistance in ultraviolet radiation environment - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: 20 minutes before radiation in the UV chamber, an infusion of nettle, birch, plantain and oak leaves, tansy blossom taken in 1:1:1:1:1 are introduced orally into laboratory animals daily at a rate of 5 ml/kg of body weight.

EFFECT: method enables providing a manifested actoprotective and antioxidant effect on the exposed animals that gives reason to recommend the above agent to increase the non-specific body resistance with underlying pro-oxidant factors.

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Description

The invention relates to medicine, in particular to pharmacology, can be used to increase the nonspecific resistance of a warm-blooded organism under ultraviolet rays and find application in experimental medicine and clinical practice.

To increase the nonspecific resistance of the body, drugs of general strengthening effect are used, based on a multifactorial effect on metabolic processes, including a wide original spectrum of pharmacological effects, an important feature of which is a combination of actoprotective (resistance to physical activity without increasing oxygen consumption) and antioxidant (resistance to active forms of oxygen) activity. It is known to use complex domestic preparations for athletes to increase physical endurance, including highly active extracts from plants, which include dioica nettle [1] and an infusion of collection from plantain leaves, calendula flowers, dioica nettle leaves in a ratio of 2: 2: 1 for increase the working capacity of laboratory animals during primary and repeated physical exertion and possessing antioxidant activity [2]. There is also a method of increasing the antioxidant status of a warm-blooded organism under ultraviolet radiation by introducing an infusion based on a collection of nettle, birch, and plantain leaves taken in a ratio of 1: 1: 1 (RF Patent No. 2424580) [3]. This technical solution was taken by us as a prototype.

The aim of the present invention was to expand the arsenal of prophylactic agents that increase the nonspecific resistance of the body and have an actoprotective and antioxidant effect, based on available domestic raw materials and increase the persistent pharmacological effect.

The problem was solved by developing a new composition based on the collection of medicinal plants: nettle leaves, birch, plantain, oak and tansy flowers, taken in a ratio of 1: 1: 1: 1: 1. The plants that make up the collection contain a wide range of biologically active substances. The raw materials used for manufacturing are available, the production technology is cost-effective, and the range of applications is wide. An infusion based on phytobox helps to increase the nonspecific resistance of the body, based on the stabilization of lipid peroxidation of biomembranes and activation of the antioxidant system, on increasing the body's resistance to physical stress, thus exerting a multifaceted positive effect on the warm-blooded organism under ultraviolet radiation and other prooxidant factors.

The technical result of the invention is to obtain an infusion based on phyto-collection from plants (nettle leaves, birch, plantain, oak and tansy flowers) and its use to increase the nonspecific resistance of a warm-blooded organism under ultraviolet radiation.

The method consists in the fact that experimental animals (rats or mice) under standard vivarium conditions, 20 minutes before irradiation in an ultraviolet chamber (RF Patent No. 2348079 of 02.27.2009), are infused daily with an infusion of nettle, birch leaves, plantain, oak and tansy flowers, taken in a ratio of 1: 1: 1: 1: 1, at the rate of 5 ml / kg weight for 21 days.

Preparation of infusion based on a collection of nettle leaves, birch, plantain, oak and tansy flowers. The leaves of nettle (harvested during flowering), birch (harvested in May), plantain (harvested in June - July), oak (harvested in June) and tansy flowers (harvested during flowering) were crushed, mixed at a ratio of 1: 1: 1 : 1: 1, poured with boiling water at the rate of 8 g per 200 ml of water, insisted for 60 minutes, filtered, the precipitate was removed, the infusion was cooled.

On the 22nd day of the experiment, the animals were killed by decapitation.

The results were taken into account by the ratio of the duration of swimming of rats in water in comparison with animals of the control group on days 7, 14 and 21 of the experiment, by the ratio of the contents of lipid peroxidation products (lipid hydroperoxides, diene conjugates, malondialdehyde) and the main components of AOS (ceruloplasmin, vitamin E) blood and internal organs in comparison with animals of the control group, processed by statistical methods using the Wilcoxon-Mann-Whitney criterion.

The method allows to provide a more pronounced increase in actoprotective and antioxidant activity compared to the prototype under conditions of a reduction in the phytocorrection course (21 days) of the processes of lipid peroxidation of biomembranes, based on a decrease in the degree of accumulation of radical products and lipid peroxides in the body of UV-irradiated mice and rats.

The duration of swimming of rats of the control and experimental groups was studied (Table 1).

As a result of the studies, the duration of swimming of animals that received an infusion on the basis of irradiation based on a collection of nettle, birch, plantain, oak and tansy flowers was significantly higher on the 7th day of the experiment compared to control (irradiated) rats by 29.3% (p <0 , 05), on day 14 - by 30% (p <0.05), on day 21 - by 32% (p <0.05). Thus, the proposed tool improves the health of the irradiated animals, which indicates the presence of the infusion based on the collection of nettle, birch, plantain, oak and tansy flowers actoprotective effect.

Table 1 The duration of swimming of rats subjected to ultraviolet radiation against the background of the introduction of the infusion based on the collection of nettle leaves, plantain, birch, oak and tansy flowers
(in minutes) Groups of animals 7 day 14 day 21 day Control Group (Ural Federal District) 99 ± 7.2 95 ± 7.1 98 ± 8.4 Experimental group 140 ± 7.8 135 ± 8.5 144 ± 10.0 (UFO + infusion) (p <0.05) (p <0.05) (p <0.05)

The content of lipid peroxidation products (lipid hydroperoxides, diene conjugates, malondialdehyde) and the main components of AOS (ceruloplasmin, vitamin E) in the blood plasma and liver tissue of the control and experimental groups was studied (Tables 2, 3, 4, 5).

table 2 The content of lipid peroxidation products in the blood plasma of rats subjected to ultraviolet radiation against the background of the introduction of the infusion based on the collection of nettle leaves, plantain, birch, oak and tansy flowers (nmol / ml) Groups of animals Lipid hydroperoxides Diene conjugates Malonic dialdehyde Control Group (Ural Federal District) 31.6 ± 2.2 40.2 ± 4.1 5.8 ± 0.4 Experimental group (UFO + infusion) 23.5 ± 1.7 25.9 ± 2.6 4.6 ± 0.2 (p <0.05) (p <0.05) (p <0.05)

As a result of the studies, the content of lipid hydroperoxides in the blood of animals that received an infusion on the basis of irradiation based on a collection of nettle, birch, plantain, oak and tansy flowers was significantly lower by 25.7% than in the control (irradiated) group of animals (p < 0.05), diene conjugates - by 35.6% (p <0.05), malondialdehyde - by 20.7% (p <0.05).

Table 3 The content of the main components of AOS in the blood of rats subjected to ultraviolet radiation against the background of the introduction of the infusion based on the collection of nettle leaves, plantain, birch, oak and tansy flowers
(mcg / ml) Groups of animals Ceruloplasmin Vitamin E Control Group (Ural Federal District) 25.4 ± 2.0 76.5 ± 6.2 Experimental group (UFO + infusion) 36.2 ± 2.6 (p <0.05) 102.4 ± 6.8 (p <0.05)

In the blood of experimental animals, the content of ceruloplasmin is significantly higher by 30% compared with the control group of rats (p <0.05), the level of vitamin E - by 25.3% (p <0.05).

Table 4 The content of lipid peroxidation products in the liver of rats subjected to ultraviolet radiation against the background of the introduction of the infusion based on the collection of nettle leaves, plantain, birch, oak and tansy flowers
(nmol / g) Groups of animals Lipid hydroperoxides Diene conjugates Malonic dialdehyde Control Group (Ural Federal District) 110.6 ± 7.0 224.2 ± 15.6 10.5 ± 1.1 Experimental group 81.2 ± 6.5 149.5 ± 12.1 6.4 ± 0.9 (UFO + infusion) (p <0.05) (p <0.05) (p <0.05)

In the liver tissue of the experimental group of animals, the content of lipid hydroperoxides was significantly lower by 26.6% compared with the control (p <0.05), diene conjugates - 34% (p <0.05), malondialdehyde - 39% (p <0.05).

Table 5 The content of the main components of AOS in the liver of rats subjected to ultraviolet radiation against the background of the introduction of the infusion based on the collection of nettle leaves, plantain, birch, oak and tansy flowers (μg / g) Groups of animals Ceruloplasmin Vitamin E Control Group (Ural Federal District) 21.5 ± 2.0 38.6 ± 2.3 Experimental group (UFO + infusion) 32.1 ± 3.2 51.2 ± 3.8 (p <0.05) (p <0.05)

The ceruloplasmin content in the liver tissue of the experimental group of animals was significantly higher by 30% compared to the control (p <0.05), the level of vitamin E was 24.7% (p <0.05).

Thus, a combination of actoprotective and antioxidant effects in conditions of ultraviolet irradiation at the infusion based on the collection of nettle leaves, plantain, birch, oak and tansy flowers was established, which gives reason to recommend for use to increase the non-specific resistance of a warm-blooded organism against the background of the influence of prooxidant factors.

Information sources

1. Turishchev S.N. Medicinal plants in sports medicine, 08/29/2007. http://www.dopinga.net/content/view.

2. Shepeleva V.V. Pharmacological study of a number of plant-derived histoprotectors for the prevention and treatment of chemotherapy complications: Author's abstract. dis ... cand. biol. sciences. - St. Petersburg, 2009 .-- 28 p.

3. Simonova N.V., Dorovskikh V.A., Anokhin R.A., Simonova I.V. A way to increase the antioxidant status of a warm-blooded organism under ultraviolet radiation. - RF patent for invention No. 2424580 of 07/20/2011; - Bulletin of inventions No. 20.

Claims (1)

A method of increasing the nonspecific resistance of an organism under ultraviolet irradiation of laboratory animals, based on a combination of actoprotective and antioxidant activity against the background of stabilization of lipid peroxidation processes of biomembranes, characterized in that animals are orally administered daily 20 minutes before exposure to ultraviolet rays at the rate of 5 ml / kg infusion based on a collection of nettle, birch, plantain, oak and tansy flowers taken in a ratio of 1: 1: 1: 1: 1, and obtained by infusion of 8 g with ora 200 ml of boiling water.