KR20200057126A - Composition for Regenerating Skin and Enhancing Hair Growth Comprising Apigenin - Google Patents
Composition for Regenerating Skin and Enhancing Hair Growth Comprising Apigenin Download PDFInfo
- Publication number
- KR20200057126A KR20200057126A KR1020180140423A KR20180140423A KR20200057126A KR 20200057126 A KR20200057126 A KR 20200057126A KR 1020180140423 A KR1020180140423 A KR 1020180140423A KR 20180140423 A KR20180140423 A KR 20180140423A KR 20200057126 A KR20200057126 A KR 20200057126A
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- South Korea
- Prior art keywords
- composition
- hair growth
- skin regeneration
- apigenin
- extract
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- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/494—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom
- A61K8/4953—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom containing pyrimidine ring derivatives, e.g. minoxidil
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/14—Drugs for dermatological disorders for baldness or alopecia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q7/00—Preparations for affecting hair growth
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2300/00—Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
Abstract
Description
본 발명은 아피게닌을 포함하는 피부 재생 및 육모 촉진용 조성물에 관한 것이다.The present invention relates to a composition for promoting skin regeneration and hair growth comprising apigenin.
피부(skin)는 인체의 최외각에 존재하여 외부 환경과 직접적으로 접해 있어, 외부의 손상으로부터 인체를 보호하고, 체내수분과 열의 과도한 증발을 막는 역할을 한다. 피부는 표피(epidermis), 진피(dermis), 피하지방(subcutaneous tissue)의 3개의 층으로 구성되어 있으며, 가장 바깥쪽에 위치하고 있는 표피의 대부분은 각질형성세포로 구성되어 있다. 표피의 각질형성세포는 기저층으로부터 증식, 이동 및 분화과정을 통해 각질을 형성하여 피부를 보호하고, 다양한 사이토카인을 생산하여 염증반응과 면역반응에 관여한다. 또한, 각질형성세포의 증식과 이동은 상처치유와 피부 재생을 제공할 뿐만 아니라, 콜라젠(collagen) 합성을 유도하여 피부의 탄력 증진과 기저막 형성을 향상시켜 피부 세포들의 부착과 증식이 조절되고, 유해물질을 선택적으로 투과하는 장벽기능의 향상과 표피와 진피의 상호작용 등을 제공한다. 이로 인해, 피부 표피 각질형성세포의 이동과 증식은 피부재생과 상처 치유뿐만 아니라 피부탄력을 증진시키고 건강한 피부가 유지되도록 도와준다.Skin exists in the outermost part of the human body and is directly in contact with the external environment, thus protecting the human body from external damage and preventing excessive evaporation of water and heat in the body. The skin is composed of three layers of epidermis, dermis, and subcutaneous tissue. Most of the outermost epidermis is composed of keratinocytes. Keratinocytes in the epidermis protect the skin by forming keratin through the process of proliferation, migration and differentiation from the base layer, and produce various cytokines to participate in inflammatory and immune responses. In addition, proliferation and migration of keratinocytes not only provides wound healing and skin regeneration, but also induces collagen synthesis to improve skin elasticity and basement membrane formation, thereby controlling adhesion and proliferation of skin cells and harmful. It provides an improvement in barrier function that selectively penetrates substances and interaction between the epidermis and the dermis. Due to this, the movement and proliferation of skin epidermal keratinocytes not only improves skin regeneration and wound healing, but also promotes skin elasticity and helps maintain healthy skin.
피부 미용에 대한 관심이 높아짐에 따라, 보다 건강하고 아름다운 피부를 유지시키기 위하여 종래로부터 다양한 동물, 식물 및, 미생물 등으로부터 얻은 안전성이 높은 각종 천연물질 유래 생리활성물질들 이용하여 피부의 고유 기능을 유지 및 증진시키고 피부세포를 활성화시켜서 피부 노화를 효과적으로 억제 내지 경감시키기 위한 다양한 시도들이 광범위하게 수행되어 왔다.As the interest in skin beauty has increased, in order to maintain a healthier and more beautiful skin, the unique function of the skin is maintained by using physiologically active substances derived from various natural substances with high safety obtained from various animals, plants, and microorganisms. And various attempts to effectively suppress or alleviate skin aging by enhancing and activating skin cells have been extensively performed.
아피게닌(apigenin)은 채소에 함유되어 있는 항산화 성분으로 잘 알려져 있는 영양성분으로, 피부 미백, 피부 재생, 모발 성장 촉진 등을 위한 성분으로서 사용되고 있다.Apigenin (apigenin) is a nutritional component well known as an antioxidant contained in vegetables, and is used as a component for skin whitening, skin regeneration, and hair growth promotion.
한국공개특허 제2017-0119757호는 아피게닌과 게르마늄 및 아미노산을 이용한 화장료 조성물로서, 각 성분이 가지는 효과들이 복합적으로 발현되는 시너지 작용에 의하여 피부 재생 및 안정화 효과를 나타낼 수 있다는 점을 개시하고 있다.Korean Patent Publication No. 2017-0119757 discloses that a cosmetic composition using apigenin, germanium, and amino acids, can exhibit skin regeneration and stabilizing effects by synergistically expressing the effects of each component.
중국등록특허 제106880693호는 아피게닌과 인삼추출물을 포함하는 조성물로서, 탈모 방지 효능이 있음을 개시하고 있다.Chinese Patent No. 106880693 discloses a composition comprising apigenin and ginseng extract, which has the effect of preventing hair loss.
이와 같이 아피게닌을 이용한 피부 재생 또는 탈모 관련 제품에 대하여 꾸준히 기술 개발이 이루어져 오고 있으나, 그 효능에 대한 소비자들의 만족도는 아직 낮은 수준이다.As such, technological development has been steadily developed for products related to skin regeneration or hair loss using apigenin, but consumers' satisfaction with the efficacy is still low.
따라서, 피부 재생과 모발 성장 촉진에 효능이 있는 아피게닌을 이용하되, 아피게닌과 시너지 작용을 하여 그 효능을 극대화할 수 있는 제품에 대한 연구개발이 지속적으로 요구되고 있다.Therefore, apigenin, which is effective in promoting skin regeneration and hair growth, is used, but research and development of products capable of maximizing its efficacy by synergistically with apigenin is continuously required.
상기 문제를 해결하기 위하여 본 발명자들은, 카페인, 인삼추출물 및 녹차추출물 중 1종 이상과 아피게닌을 동시에 포함하는 조성물을 제조하였으며, 상기 조성물이 피부 재생 및 육모 성장 촉진 효능이 우수한 것을 확인하였다.In order to solve the above problems, the present inventors have prepared a composition containing at least one of caffeine, ginseng extract, and green tea extract and apigenin at the same time, and confirmed that the composition has excellent efficacy in promoting skin regeneration and hair growth.
따라서, 본 발명의 목적은 아피게닌을 포함하는 피부 재생 및 육모 촉진용 조성물을 제공하는데 있다.Accordingly, an object of the present invention is to provide a composition for promoting skin regeneration and hair growth comprising apigenin.
상기 목적을 달성하기 위하여, 본 발명은 아피게닌(apigenin); 및 카페인, 인삼 추출물 및 녹차 추출물로 이루어진 군에서 선택되는 1종 이상;을 포함하는 피부 재생 및 육모 촉진용 조성물을 제공한다.In order to achieve the above object, the present invention apigenin (apigenin); And caffeine, ginseng extract and one or more selected from the group consisting of green tea extract; provides a composition for promoting skin regeneration and hair growth comprising a.
상기 아피게닌은 상기 조성물 전체 중량을 기준으로 40 내지 60 중량%로 포함되는 것일 수 있다.The apigenin may be contained in 40 to 60% by weight based on the total weight of the composition.
상기 인삼 추출물 및 녹차추출물로 이루어진 군에서 선택되는 1종 이상은 상기 피부 재생용 조성물 전체 중량을 기준으로 40 내지 60 중량%로 포함되는 것일 수 있다.One or more selected from the group consisting of the ginseng extract and green tea extract may be included in 40 to 60% by weight based on the total weight of the composition for skin regeneration.
상기 조성물은 윈트/베타-카테닌 신호(Wnt/beta-catenin signal)를 활성화하는 것일 수 있다.The composition may be to activate the Wnt / beta-catenin signal.
상기 조성물의 농도는 고형분 농도를 기준으로 10 내지 40 ppm인 것일 수 있다.The concentration of the composition may be 10 to 40 ppm based on the solid content concentration.
상기 조성물은 아피게닌 및 카페인을 포함하는 것일 수 있다.The composition may include apigenin and caffeine.
상기 조성물은 아피게닌 및 인삼 추출물을 포함하는 것일 수 있다.The composition may include apigenin and ginseng extract.
상기 조성물은 아피게닌 및 녹차 추출물을 포함하는 것일 수 있다.The composition may include apigenin and green tea extract.
상기 조성물은 화장료 조성물, 식품 조성물 및 약학 조성물로 이루어진 군에서 선택되는 1종 이상인 것일 수 있다.The composition may be one or more selected from the group consisting of cosmetic compositions, food compositions and pharmaceutical compositions.
본 발명의 피부 재생 및 육모 성장 촉진용 조성물은 아피게닌과 함께, 카페인, 인삼추출물 및 녹차추출물 중 1종 이상을 포함함으로써, 각 성분이 가지는 효능의 시너지 작용으로 인하여 피부 재생 효과, 구체적으로 뛰어난 피부 상처 치유 및 흉터 감소 효과를 나타낼 수 있다.The composition for promoting skin regeneration and growth of hair growth of the present invention includes apigenin, at least one of caffeine, ginseng extract, and green tea extract, resulting in synergistic effect of the efficacy of each component, specifically excellent skin It can exhibit wound healing and scar reduction effects.
또한, 상기 피부 재생 및 육모 성장 촉진용 조성물은 모발 성장을 촉진하여 탈모를 예방 또는 개선할 수 있는 효과가 있다.In addition, the composition for promoting skin regeneration and growth of hair has an effect of preventing or improving hair loss by promoting hair growth.
또한, 상기 피부 재생 및 육모 성장 촉진용 조성물은 윈트/베타-카테닌(Wnt/beta-catenin) 신호전달을 통하여 세포 분화를 촉진하므로, 피부 재생 및 육모 성장 촉진 효과를 더욱 개선시킬 수 있다.In addition, the composition for promoting skin regeneration and hair growth promotes cell differentiation through signaling of Wnt / beta-catenin, thereby further improving skin regeneration and hair growth growth promoting effects.
또한, 상기 피부 재생 및 육모 성장 촉진용 조성물은 피부 재생 및 모발 성장 촉진 효능을 가지는 화장료 조성물, 식품 조성물 및 약학 조성물에 광범위하게 적용될 수 있다.In addition, the composition for promoting skin regeneration and hair growth can be widely applied to cosmetic compositions, food compositions, and pharmaceutical compositions having skin regeneration and hair growth promoting effects.
도 1은 실시예 및 비교예에서 각각 제조된 조성물의 Wnt 신호 전달 활성을 나타낸 그래프이다.1 is a graph showing Wnt signal transduction activity of compositions prepared in Examples and Comparative Examples, respectively.
이하, 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자가 용이하게 실시할 수 있도록 상세히 설명한다. 그러나 본 발명은 여러 가지 상이한 형태로 구현될 수 있으며, 여기에서 설명하는 실시예에 한정되지 않는다.Hereinafter, it will be described in detail so that those skilled in the art to which the present invention pertains can easily practice. However, the present invention may be implemented in various different forms, and is not limited to the embodiments described herein.
피부 재생 및 육모 촉진용 조성물Composition for skin regeneration and hair growth promotion
본 발명은 피부 재생 및 육모 촉진용 조성물에 관한 것으로, 상기 피부 재생 및 육모 촉진용 조성물은 아피게닌(apigenin); 및 카페인, 인삼 추출물 및 녹차 추출물로 이루어진 군에서 선택되는 1종 이상;을 포함한다.The present invention relates to a composition for promoting skin regeneration and hair growth, wherein the composition for promoting skin regeneration and hair growth comprises apigenin; And one or more selected from the group consisting of caffeine, ginseng extract, and green tea extract.
상기 아피게닌과, 카페인, 인삼 추출물 및 녹차 추출물로 이루어진 군에서 선택되는 1종 이상은 각각 피부 재생 또는 육모 성장 촉진 효능을 가지고 있으나, 이들을 조합할 경우 이들 성분의 시너지 작용에 의해 피부 재생 또는 육모 성장 촉진 효능이 현저히 향상시킬 수 있다.At least one selected from the group consisting of the apigenin, caffeine, ginseng extract, and green tea extract has an effect of promoting skin regeneration or hair growth, but when combined, skin regeneration or hair growth by synergy of these ingredients The promoting effect can be significantly improved.
바람직하게는, 상기 피부 재생 및 육모 촉진용 조성물은 아피게닌과 카페인을 포함하거나, 또는 아피게닌과 인삼 추출물을 포함하거나, 또는 아피게닌과 녹차 추출물의 조합을 포함할 수 있다. 상기 피부 재생 및 육모 촉진용 조성물이 이와 같은 조합의 성분을 포함할 경우 피부 재생 및 육모 성장 촉진을 위한 시너지 작용이 가장 우수할 수 있다.Preferably, the composition for promoting skin regeneration and hair growth may include apigenin and caffeine, or apigenin and ginseng extract, or a combination of apigenin and green tea extract. When the composition for promoting skin regeneration and hair growth includes components of such a combination, synergy for promoting skin regeneration and hair growth may be the best.
본 발명에 있어서, 상기 아피게닌은 과일, 채소 또는 허브에 포함된 플라보노이드 성분의 일종으로, 이들 과일, 채소 또는 허브로부터 추출된 것일 수 있다.In the present invention, the apigenin is a kind of flavonoid component contained in a fruit, vegetable or herb, and may be extracted from these fruits, vegetables or herbs.
상기 아피게닌은 상기 피부 재생 또는 육모 촉진용 조성물 전체 중량을 기준으로 40 내지 60 중량%, 바람직하게는 42 내지 58 중량%, 보다 바람직하게는 45 내지 55 중량%로 포함될 수 있다. 상기 범위 미만이거나 상기 범위 초과이면 상기 카페인, 인삼 추출물 및 녹차 추출물로 이루어진 군에서 선택되는 1종 이상과의 시너지 작용 효과가 저하될 수 있다.The apigenin may be included in an amount of 40 to 60% by weight, preferably 42 to 58% by weight, and more preferably 45 to 55% by weight based on the total weight of the composition for promoting skin regeneration or hair growth. If it is less than or equal to the above range, the synergistic effect with one or more selected from the group consisting of the caffeine, ginseng extract and green tea extract may be lowered.
본 발명에 있어서, 상기 카페인, 인삼 추출물 및 녹차 추출물로 이루어진 군에서 선택되는 1종 이상은 상기 아피게논과의 조합에 의해 피부 재생 및 육모 성장 촉진을 위한 시너지 작용을 나타낼 수 있다.In the present invention, at least one selected from the group consisting of the caffeine, ginseng extract and green tea extract may exhibit a synergistic effect for promoting skin regeneration and hair growth by combining with the apigenone.
상기 카페인, 인삼 추출물 및 녹차 추출물로 이루어진 군에서 선택되는 1종 이상은 상기 피부 재생 또는 육모 촉진용 조성물 전체 중량을 기준으로 40 내지 60 중량%, 바람직하게는 42 내지 58 중량%, 보다 바람직하게는 45 내지 55 중량%로 포함될 수 있다. 상기 범위 미만이거나 상기 범위 초과이면 상기 아피게논과 시너지 작용 효과가 저하될 수 있다.One or more selected from the group consisting of the caffeine, ginseng extract and green tea extract is 40 to 60% by weight, preferably 42 to 58% by weight, more preferably based on the total weight of the composition for promoting skin regeneration or hair growth 45 to 55% by weight. If it is less than or equal to the above range, the synergistic effect with the apigenone may be reduced.
상기 카페인은 특별히 제한되지 않지만, 합성 카페인; 또는 과라나추출물, 커피 추출물 및 차 추출물로 이루어진 군으로부터 선택되는 1종 이상의 천연 카페인 추출물의 형태를 사용할 수 있다.The caffeine is not particularly limited, synthetic caffeine; Alternatively, a form of at least one natural caffeine extract selected from the group consisting of guarana extract, coffee extract and tea extract may be used.
상기 카페인은 그 농도가 고형분 농도를 기준으로 5 내지 20 ppm, 바람직하게는 5 내지 15 ppm, 바람직하게는 7 내지 12 ppm 일 수 있다. 상기 범위 미만이면 피부 재생 및 육모 성장 촉진 효과가 미미할 수 있고, 상기 범위 초과이면 아피게논과의 시너지 작용을 위한 최적의 농도를 초과하게 되어 역시 피부 재생 및 육모 성장 촉진 효과가 저하될 수 있다. 이때, 카페인의 농도란 합성 카페인의 경우 카페인 수용액의 농도를 의미하고, 천연 카페인 추출물 형태일 경우 고형분 농도를 의미한다.The caffeine may have a concentration of 5 to 20 ppm, preferably 5 to 15 ppm, and preferably 7 to 12 ppm, based on the solid content concentration. If it is less than the above range, the effect of promoting skin regeneration and hair growth may be negligible, and if it is more than the above range, the optimum concentration for synergistic action with apigenone may be exceeded, and thus the effect of promoting skin regeneration and hair growth may be reduced. At this time, the concentration of caffeine means the concentration of an aqueous solution of caffeine in the case of synthetic caffeine, and the concentration of solid content in the form of a natural caffeine extract.
상기 인삼 추출물은 인삼뿌리 추출물, 인삼잎 추출물, 인삼열매 추출물 및 인삼씨 추출물로 이루어진 군에서 선택되는 1종 이상일 수 있으며, 바람직하게는 인삼 뿌리 추출물일 수 있다.The ginseng extract may be one or more selected from the group consisting of ginseng root extract, ginseng leaf extract, ginseng fruit extract, and ginseng seed extract, preferably ginseng root extract.
상기 인삼 추출물은 그 농도가 고형분 농도를 기준으로 5 내지 20 ppm, 바람직하게는 5 내지 15 ppm, 바람직하게는 7 내지 12 ppm 일 수 있다. 상기 범위 미만이면 피부 재생 및 육모 성장 촉진 효과가 미미할 수 있고, 상기 범위 초과이면 아피게논과의 시너지 작용을 위한 최적의 농도를 초과하게 되어 역시 피부 재생 및 육모 성장 촉진 효과가 저하될 수 있다.The ginseng extract may have a concentration of 5 to 20 ppm, preferably 5 to 15 ppm, and preferably 7 to 12 ppm based on the solid content concentration. If it is less than the above range, the effect of promoting skin regeneration and hair growth may be negligible, and if it is more than the above range, the optimum concentration for synergistic action with apigenone may be exceeded, and thus the effect of promoting skin regeneration and hair growth may be reduced.
또한, 상기 인삼 추출물은 물, 유기용매 및 상기 물과 유기용매의 혼합용매로 이루어진 군에서 선택된 추출 용매를 이용하여 추출된 것일 수 있다. 상기 유기용매는 에탄올, 메탄올, 부탄올, 에테르, 에틸아세테이트 및 클로로포름으로 이루어진 군에서 선택된 1종 이상일 수 있다. 바람직하게는 상기 추출 용매는 에탄올일 수 있다.In addition, the ginseng extract may be extracted using an extraction solvent selected from the group consisting of water, an organic solvent and a mixed solvent of water and an organic solvent. The organic solvent may be one or more selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate and chloroform. Preferably, the extraction solvent may be ethanol.
또한, 상기 인삼 추출물을 제조하는 방법은 당업계에서 사용되는 통상의 추출 방법이라면 특별히 제한되지 않으나, 예를 들면, 상기 추출 용매를 이용하여 10~80 ℃에서 3~24시간 동안 추출하여 제조될 수 있다.In addition, the method for preparing the ginseng extract is not particularly limited as long as it is a common extraction method used in the art, for example, it can be prepared by extracting for 3 to 24 hours at 10 to 80 ° C. using the extraction solvent. have.
상기 녹차 추출물은 녹차잎 추출물, 녹차꽃 추출물 및 녹차줄기 추출물로 이루어진 군에서 선택되는 1종 이상일 수 있으며, 바람직하게는 녹차잎 추출물일 수 있다.The green tea extract may be at least one selected from the group consisting of green tea leaf extract, green tea flower extract and green tea stem extract, preferably green tea leaf extract.
상기 녹차 추출물은 그 농도가 고형분 농도를 기준으로 5 내지 20 ppm, 바람직하게는 5 내지 15 ppm, 바람직하게는 7 내지 12 ppm 일 수 있다. 상기 범위 미만이면 피부 재생 및 육모 성장 촉진 효과가 미미할 수 있고, 상기 범위 초과이면 아피게논과의 시너지 작용을 위한 최적의 농도를 초과하게 되어 역시 피부 재생 및 육모 성장 촉진 효과가 저하될 수 있다.The green tea extract may have a concentration of 5 to 20 ppm, preferably 5 to 15 ppm, and preferably 7 to 12 ppm, based on the solid content concentration. If it is less than the above range, the effect of promoting skin regeneration and hair growth may be negligible, and if it is more than the above range, the optimum concentration for synergistic action with apigenone may be exceeded, and thus the effect of promoting skin regeneration and hair growth may be reduced.
또한, 상기 녹차 추출물은 열수 추출물 또는 C1 내지 C5의 저급알코올 추출물일 수 있다. 바람직하게는, 상기 녹차 추출물은 에탄올 추출물일 수 있다. In addition, the green tea extract may be a hot water extract or a lower alcohol extract of C 1 to C 5 . Preferably, the green tea extract may be an ethanol extract.
또한, 상기 녹차 추출물을 제조하는 방법은 당업계에서 사용되는 통상의 추출 방법이라면 특별히 제한되지 않는다. 예를 들면, 상기 녹차 추출물이 열수 추출물일 경우에는 녹차 투입, 열수추출, 여과, 감압농축 및 분무건조의 과정을 거쳐 얻을 수 있다. 또는 상기 녹차 추출물이 에탄올 추출물일 경우 에탄올을 이용하여 10~80 ℃에서 3~24시간 동안 추출하여 제조될 수 있다.In addition, the method for preparing the green tea extract is not particularly limited as long as it is a conventional extraction method used in the art. For example, when the green tea extract is a hot water extract, it may be obtained through the processes of green tea input, hot water extraction, filtration, concentration under reduced pressure, and spray drying. Alternatively, if the green tea extract is an ethanol extract, it may be prepared by extracting for 3 to 24 hours at 10 to 80 ° C using ethanol.
본 발명에 있어서, 상기 피부 재생 및 육모 촉진용 조성물의 농도는 고형분 농도를 기준으로 10 내지 40 ppm, 바람직하게는 10 내지 30 ppm, 보다 바람직하게는 15 내지 25 ppm 일 수 있다. 상기 범위 미만이거나 초과이면 피부 재생 및 육모 성장 촉진 효과가 저하될 수 있다. 또한, 상기 조성물을 상기 농도 범위일 경우, 후술하는 바와 같은 윈트/베타-카테닌 신호 활성화를 가능하게 할 수 있다.In the present invention, the concentration of the composition for promoting skin regeneration and hair growth may be 10 to 40 ppm, preferably 10 to 30 ppm, more preferably 15 to 25 ppm, based on the solid content concentration. If it is less than or equal to the above range, the effect of promoting skin regeneration and hair growth may be reduced. In addition, when the composition is in the concentration range, it is possible to enable the win / beta-catenin signal activation as described below.
또한, 상기 피부 재생 및 육모 촉진용 조성물은 윈트/베타-카테닌 신호(Wnt/beta-catenin signal)를 활성화하여, 피부 재생 및 육모 성장을 촉진할 수 있다.In addition, the composition for promoting skin regeneration and hair growth can promote skin regeneration and hair growth by activating a Wnt / beta-catenin signal.
윈트/베타-카테닌(Wnt/beta-catenin) 신호 전달은 생명체의 발생과 관련 있는 신호 전달 체계이며, 피부 생성 및 모낭의 발생에 작용하는 것으로 알려져 있다. 나아가 피부에 상처가 생기면 피부 조직 회복 과정에서 윈트 신호 전달이 필수적으로 발현되어 상피 세포의 분화를 촉진하는 것으로 알려져 있다(Fathke et al., "Wnt signaling induces epithelial differentiation during cutaneous wound healing", BMC Cell Biology 2006, 7:4). 따라서 윈트 신호 전달이 촉진되면 상피 세포의 분화가 촉진되어 피부 재생이 촉진될 수 있고, 나아가 피부 상처의 치유가 촉진될 수 있다. 치자 추출물은 줄기세포의 윈트/베타-카테닌(Wnt/beta-catenin) 신호 전달을 촉진한다. 따라서 이를 포함하는 제제는 피부 재생을 촉진할 수 있으며, 구체적으로 피부 상처의 치유를 촉진할 수 있다 (Kateri A. Moore et al., "Stem Cells and Their Niches", Science, VOL 311, p1880-1885).Wnt / beta-catenin signaling is a signaling system related to the development of living organisms and is known to act on skin formation and hair follicle development. Furthermore, when a wound is formed on the skin, it is known that wind signal transmission is essentially expressed in the process of skin tissue recovery to promote differentiation of epithelial cells (Fathke et al ., "Wnt signaling induces epithelial differentiation during cutaneous wound healing", BMC Cell Biology 2006, 7: 4). Therefore, when the wind signal transmission is promoted, differentiation of epithelial cells is promoted, skin regeneration can be promoted, and further, healing of skin wounds can be promoted. Gardenia extract promotes stem cell wint / beta-catenin signaling. Therefore, formulations containing the same can promote skin regeneration and specifically promote healing of skin wounds (Kateri A. Moore et al ., "Stem Cells and Their Niches", Science, VOL 311, p1880-1885 ).
본 발명에 있어서, 상기 피부 재생 및 육모 성장 촉진용 조성물은 화장료 조성물, 식품 조성물 및 약학 조성물로 이루어진 군에서 선택되는 1종 이상일 수 있다.In the present invention, the composition for promoting skin regeneration and hair growth may be at least one selected from the group consisting of cosmetic compositions, food compositions and pharmaceutical compositions.
상기 피부 재생 및 육모 성장 촉진용 조성물이 화장료 조성물일 경우, 화장품학 또는 피부과학적으로 허용 가능한 매질 또는 기제를 함유하여 제형화될 수 있다. 또한, 상기 피부 재생 및 육모 성장 촉진용 화장료 조성물은 국소 적용에 적합한 모든 제형으로 제공될 수 있으며, 예를 들어 용액, 수상에 유상을 분산시켜 얻은 에멀젼, 유상에 수상을 분산시켜 얻은 에멀젼, 현탁액, 고체, 겔, 분말, 페이스트, 포말(foam) 또는 에어로졸 조성물의 제형으로 제공될 수 있다. 이러한 제형의 조성물은 당해 분야의 통상적인 방법에 따라 제조될 수 있다.When the composition for promoting skin regeneration and hair growth is a cosmetic composition, it may be formulated by containing a cosmetic or dermatologically acceptable medium or base. In addition, the cosmetic composition for promoting skin regeneration and hair growth may be provided in any formulation suitable for topical application, for example, a solution, an emulsion obtained by dispersing an oil phase in an aqueous phase, an emulsion obtained by dispersing an aqueous phase in an oil phase, a suspension, It can be provided in the form of a solid, gel, powder, paste, foam or aerosol composition. Compositions of these formulations can be prepared according to conventional methods in the art.
또한, 상기 피부 재생 및 육모 성장 촉진용 화장료 조성물은 상기한 물질 이외에 주 효과를 손상시키지 않는 범위 내에서, 바람직하게는 주 효과에 상승 효과를 줄 수 있는 다른 성분들을 포함할 수 있다. 또한 상기 피부 재생 및 육모 성장 촉진용 화장료 조성물은 보습제, 에몰리언트제, 자외선 흡수제, 방부제, 살균제, 산화 방지제, pH 조정제, 유기 및 무기 안료, 향료, 냉감제 또는 제한제를 더 포함할 수 있다. 상기 성분의 배합량은 본 발명의 목적 및 효과를 손상시키지 않는 범위 내에서 당업자가 용이하게 선정 가능하며, 그 배합량은 조성물 총 중량에 대하여 0.01~5중량%, 구체적으로 0.01~3중량%일 수 있다.In addition, the cosmetic composition for promoting skin regeneration and growth of hair growth may include other ingredients that can give a synergistic effect to the main effect, within a range that does not impair the main effect other than the above-mentioned substances. In addition, the cosmetic composition for promoting skin regeneration and hair growth may further include a moisturizing agent, an emollient, an ultraviolet absorbent, a preservative, a disinfecting agent, an antioxidant, a pH adjusting agent, organic and inorganic pigments, flavoring agents, cooling agents, or limiting agents. The compounding amount of the components can be easily selected by those skilled in the art within a range that does not impair the objects and effects of the present invention, and the blending amount may be 0.01 to 5% by weight, specifically 0.01 to 3% by weight, based on the total weight of the composition. .
상기 피부 재생 및 육모 성장 촉진용 조성물이 식품 조성물일 경우, 다양한 형태의 식품 첨가제 또는 기능성 식품에 적용될 수 있다. 상기 피부 재생 및 육모 성장 촉진용 식품 조성물을 포함하는 발효유, 치즈, 요구르트, 주스, 생균제제 및 건강보조식품 등으로 가공될 수 있으며, 그 외 다양한 식품 첨가제의 형태로 사용될 수 있다.When the composition for promoting skin regeneration and hair growth is a food composition, it may be applied to various types of food additives or functional foods. It can be processed into fermented milk, cheese, yogurt, juice, probiotic and health supplements including the food composition for promoting skin regeneration and hair growth, and can be used in the form of various other food additives.
또한, 상기 피부 재생 및 육모 성장 촉진용 식품 조성물은, 본 발명이 목적으로 하는 주 효과를 손상시키지 않는 범위 내에서 주 효과에 상승 효과를 줄 수 있는 다른 성분 등을 함유할 수 있다. 예를 들어, 물성 개선을 위하여 향료, 색소, 살균제, 산화방지제, 방부제, 보습제, 점증제, 무기염류, 유화제 및 합성 고분자 물질 등의 첨가제를 더 포함할 수 있다. 그 외에도, 수용성 비타민, 유용성 비타민, 고분자 펩티드, 고분자 다당 및 해초 엑기스 등의 보조 성분을 더 포함할 수도 있다. 상기 성분들은 제형 또는 사용 목적에 따라서 당업자가 어려움 없이 적의 선정하여 배합할 수 있으며, 그 첨가량은 본 발명의 목적 및 효과를 손상시키지 않는 범위 내에서 선택될 수 있다. 예를 들어, 상기 성분들의 첨가량은, 조성물 전체 중량을 기준으로, 0.01~5 중량%, 보다 구체적으로는 0.01~3 중량% 범위일 수 있다.In addition, the food composition for promoting skin regeneration and hair growth may contain other ingredients and the like that can give a synergistic effect to the main effect within a range that does not impair the main effect targeted by the present invention. For example, in order to improve physical properties, additives such as fragrances, pigments, disinfectants, antioxidants, preservatives, moisturizers, thickeners, inorganic salts, emulsifiers, and synthetic polymer materials may be further included. In addition, auxiliary components such as water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, and seaweed extract may be further included. The ingredients may be appropriately selected and blended without difficulty by a person skilled in the art depending on the formulation or the purpose of use, and the amount added may be selected within a range not impairing the objects and effects of the present invention. For example, the addition amount of the components, based on the total weight of the composition, may be in the range of 0.01 to 5% by weight, more specifically 0.01 to 3% by weight.
본 발명에 따른 상기 피부 재생 및 육모 성장 촉진용 식품 조성물은 용액, 유화물, 점성형 혼합물, 타블렛, 분말 등의 다양한 형태로 제형화될 수 있으며, 이는 단순 음용, 주사 투여, 스프레이 방식 또는 스퀴즈 방식 등의 다양한 방법으로 투여될 수 있다.The food composition for promoting skin regeneration and hair growth according to the present invention may be formulated in various forms such as solutions, emulsions, viscous mixtures, tablets, powders, etc., such as simple drinking, injection, spray, or squeeze methods. It can be administered in a variety of ways.
상기 피부 재생 및 육모 성장 촉진용 조성물이 약학 조성물일 경우, 의약품에 적용될 수 있으며, 본 발명에서 사용되는 유효성분에 상용되는 무기 또는 유기의 담체를 가하여 고체, 반고체 또는 액상의 형태로 경구 투여제 혹은 비경구 투여제로 제형화할 수 있으며, 본 발명에 따른 상기 약학 조성물은 경구, 비경구, 직장, 국소, 경피, 정맥 내, 근육 내, 복강 내, 피하 등으로 투여될 수 있다.When the composition for promoting skin regeneration and growth of hair growth is a pharmaceutical composition, it can be applied to pharmaceuticals, orally or in a solid, semi-solid or liquid form by adding an inorganic or organic carrier compatible with the active ingredient used in the present invention, or It can be formulated as a parenteral administration agent, and the pharmaceutical composition according to the present invention can be administered orally, parenterally, rectal, topical, transdermal, intravenous, intramuscular, intraperitoneal, subcutaneous, and the like.
상기 경구 투여를 위한 제형으로서는 정제, 환제, 과립제, 캡슐제, 산제, 세립제, 분제, 유탁제, 시럽제, 펠렛제 등을 들 수 있다. 또한, 상기 비경구 투여를 위한 제형으로는 주사제, 점적제, 연고, 로션, 스프레이, 현탁제, 유제, 좌제 등을 들 수 있다. 본 발명에 따른 조성물은 상법에 따라 실시함으로써, 유효성분을 용이하게 제형화할 수 있으며, 이 때 계면활성제, 부형제, 착색료, 향신료, 보존료, 안정제, 완충제, 현탁제, 기타 상용하는 보조제를 적당히 사용할 수 있다.Examples of the dosage form for oral administration include tablets, pills, granules, capsules, powders, granules, powders, emulsions, syrups, pellets, and the like. In addition, formulations for parenteral administration include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. The composition according to the present invention can be easily formulated into an active ingredient by carrying out according to the commercial method, in which case surfactants, excipients, colorants, spices, preservatives, stabilizers, buffers, suspending agents, and other commonly used adjuvants can be suitably used. have.
또한, 본 발명의 약학 조성물의 유효 성분의 투여 용량은 투여받을 대상의 연령, 성별, 체중, 병리 상태 및 그 심각도, 투여 경로 또는 처방자의 판단에 따라 달라질 것이다. 이러한 인자에 기초한 적당한 용량의 결정은 당업자의 수준 내에 있으며, 이의 1일 투여 용량은 예를 들어 0.1mg/kg/일 내지 100mg/kg/일, 보다 구체적으로는 5mg/kg/일 내지 50mg/kg/일이 될 수 있으나, 이에 제한되는 것은 아니다.In addition, the dosage of the active ingredient of the pharmaceutical composition of the present invention will vary depending on the age, gender, weight, pathology and severity of the subject to be administered, the route of administration or the judgment of the prescriber. Determination of a suitable dose based on these factors is within the level of those skilled in the art, and the daily dose administered is, for example, 0.1 mg / kg / day to 100 mg / kg / day, more specifically 5 mg / kg / day to 50 mg / kg. / Can be a day, but is not limited to this.
전술한 바와 같은 피부 재생 및 육모 성장 촉진용 조성물은 아피게닌과 함께, 카페인, 인삼추출물 및 녹차추출물 중 1종 이상을 포함함으로써, 각 성분이 가지는 효능의 시너지 작용으로 인하여 피부 재생 효과, 구체적으로 뛰어난 피부 상처 치유 및 흉터 감소 효과를 나타내고, 육모 성장 촉진 효과를 나타내어, 이와 같은 효능을 가지는 화장품, 식품 및 의약품 분야에 광범위하게 적용될 수 있다.The composition for promoting skin regeneration and hair growth as described above includes apigenin and at least one of caffeine, ginseng extract, and green tea extract, thereby synergistically acting on the efficacy of each component, thereby providing excellent skin regeneration effect. It has the effect of healing skin wounds and reducing scars, and has an effect of promoting hair growth, and thus can be widely applied to the fields of cosmetics, food, and pharmaceuticals having such effects.
이하 본 발명의 이해를 돕기 위하여 바람직한 실시예를 제시하나, 하기 실시예는 본 발명을 예시하는 것일 뿐 본 발명의 범주 및 기술사상 범위 내에서 다양한 변경 및 수정이 가능함은 통상의 기술자에게 있어서 명백한 것이며, 이러한 변경 및 수정이 첨부된 특허청구범위에 속하는 것도 당연한 것이다.Hereinafter, preferred examples are provided to help understanding of the present invention, but the following examples are merely illustrative of the present invention, and it is apparent to those skilled in the art that various changes and modifications are possible within the scope and technical thought scope of the present invention. It is natural that such changes and modifications fall within the scope of the appended claims.
제조예 1: 아피게닌 제조Preparation Example 1: Preparation of apigenin
엉겅퀴(circium japonicum)는 충북지방 산간 지역에서 채취 건조하여 사용하였다. 경동시장(서울, 한국)에서 구입한 것을 건조하여 사용하였다. 건조시킨 엉겅퀴(circium japonicum)는 95% 주정을 가하여 추출하고 감압 농축 장치로 농축하여 주정 조 추출물을 제조하였다.Thistle (circium japonicum) was collected and used in mountainous areas of Chungbuk Province. What was purchased from Gyeongdong Market (Seoul, Korea) was dried and used. The dried thistle (circium japonicum) was extracted by adding 95% alcohol, and concentrated using a vacuum concentrator to prepare a spirit crude extract.
상기 주정 조 추출물은 증류수와 에틸아세테이트에 용해하여 1 : 1의 비율(v/v)로 혼합하여 이상간 분배를 3회 반복하고, 에틸아세테이트 추출물을 감압, 농축, 건조하였다.The crude crude extract was dissolved in distilled water and ethyl acetate, mixed at a ratio of 1: 1 (v / v), and the distribution between the abnormalities was repeated three times, and the ethyl acetate extract was reduced, concentrated, and dried.
상기 건조물에 대해, 실리카겔 플래쉬 크로마토 그래피 (용매계:클로로포름/에틸아세테이트 = 2 : 1)를 수행하여 활성분획을 다시 감압, 건조한 다음, 소량의 메탄올에 포화상태로 용해시켜 밀리포아(0.45nm)로 여과하여 고성능 분석기기(HPLC, 칼럼: 오디에스 20×250nm; 80% 메탄올)로 활성물질인 아피게닌을 분취하였다.The dried product was subjected to silica gel flash chromatography (solvent system: chloroform / ethyl acetate = 2: 1) to reduce the active fraction again under reduced pressure, dry, and then dissolve in a small amount of methanol saturated with millipore (0.45 nm). Filtration was performed to collect apigenin as an active material with a high-performance analyzer (HPLC, column: ODS 20 × 250 nm; 80% methanol).
제조예 2: 카페인 제조Preparation Example 2: Caffeine production
카페인은 시그마 화학 주식회사(St. Louis, MO, USA)로부터 구매하였다.Caffeine was purchased from Sigma Chemical Corporation (St. Louis, MO, USA).
제조예 3: 인삼 추출물 제조Preparation Example 3: Preparation of ginseng extract
건조된 백삼 1kg을 믹서기로 분쇄한 뒤 여기에 정제수 5ℓ를 넣고, 38~42℃ 온수로 24시간 교반 추출한 다음, 15℃에서 1일간 침적시켰다. 그 후, 여과포 여과와 원심분리를 통해 잔사와 여액을 분리하고, 분리된 여액을 감압농축하여 그 추출물 270g을 얻었다.After crushing 1 kg of dried white ginseng with a blender, 5 liters of purified water was added thereto, and stirred and extracted with hot water at 38 to 42 ° C. for 24 hours, and then deposited at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filtration and filtration and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 270 g of the extract.
제조예 4: 녹차 추출물 제조Preparation Example 4: Preparation of green tea extract
1차 가공된(덖음 과정) 녹차 잎 1kg을 믹서기로 분쇄한 뒤 여기에 정제수 5ℓ를 넣고, 38~42℃ 온수로 24시간 교반 추출한 다음, 15℃에서 1일간 침적시켰다. 그 후, 여과포 여과와 원심분리를 통해 잔사와 여액을 분리하고, 분리된 여액을 감압 농축하여 그 추출물 290g을 얻었다.After crushing 1 kg of green tea leaves that were primarily processed (steaming process) with a blender, 5 l of purified water was added thereto, and the mixture was stirred for 24 hours with hot water at 38 to 42 ° C., and then deposited at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filtration and filtration and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 290 g of the extract.
실시예 1Example 1
제조예 1 및 제조예 2에서 각각 제조된 아피게닌 50 중량% 및 카페인 50 중량%를 혼합하여, 20 ppm 의 조성물을 제조하였다.Apigenin 50% by weight and caffeine 50% by weight, respectively prepared in Preparation Example 1 and Preparation Example 2, were mixed to prepare a 20 ppm composition.
실시예 2Example 2
제조예 1 및 제조예 3에서 각각 제조된 아피게닌 50 중량% 및 인삼 추출물 50 중량%를 혼합하여, 20 ppm 의 조성물을 제조하였다.Apigenin 50 wt% and ginseng extract 50 wt% prepared in Preparation Example 1 and Preparation Example 3, respectively, were mixed to prepare a 20 ppm composition.
실시예 3Example 3
제조예 1 및 제조예 3에서 각각 제조된 아피게닌 50 중량% 및 녹차 추출물 50 중량%를 혼합하여, 20 ppm 의 조성물을 제조하였다.Apigenin 50 wt% and green tea extract 50 wt%, respectively prepared in Preparation Example 1 and Preparation Example 3, were mixed to prepare a 20 ppm composition.
비교예 1 내지 비교예 8Comparative Examples 1 to 8
비교예 1 내지 비교예 8 에 대하여, 하기 표 1에 기재된 바와 같은 조성물을 각각 20 ppm 으로 준비하였다.For Comparative Examples 1 to 8, compositions as described in Table 1 below were prepared at 20 ppm each.
실험예 1 Experimental Example 1
표피 줄기세포에 Wnt 신호가 주어지면 세포 내 베타-카테닌(beta-catenin)을 억제하고 있는 단백질 복합체(GSK3, APC, Axin)가 저해되면서 베타-카테닌이 세포 핵 내로 이동하고 발생 및 모발 성장에 관여하는 다양한 유전자가 발현되는 원리를 이용하여, 실시예 및 비교예의 조성물에 의한 Wnt 신호 전달 정도에 대한 실험을 실시하였다. 구체적인 실험 방법은 아래와 같다.When Wnt signal is given to epidermal stem cells, the protein complex (GSK3, APC, Axin) that inhibits intracellular beta-catenin is inhibited, and beta-catenin moves into the cell nucleus and is involved in development and hair growth Using the principle in which various genes are expressed, experiments were performed on the degree of Wnt signal transmission by the compositions of Examples and Comparative Examples. The specific experimental method is as follows.
[Wnt 신호 전달 실험][Wnt signal transmission experiment]
(1)세포 배양(1) Cell culture
Wnt reporter cell line을 구매한 후 (#ENZ-61002-0001) Collagen typeⅠ이 coating된 white/clear flat bottom의 96well plate에 Wnt reporter cell line을 1x104 cells/well로 시딩(seeding)하였다. After purchasing the Wnt reporter cell line (# ENZ-61002-0001), the Wnt reporter cell line was seeded at 1x10 4 cells / well in a 96well plate on a white / clear flat bottom coated with Collagen type I.
(2)물질 처리(2) Material treatment
무혈청 배지(Serum free medium)를 사용하여 24시간 동안 실시예 및 비교예의 조성물을 농도별로 100 ㎕ 씩 처리하였다. Using the serum free medium (Serum free medium) for 24 hours, the compositions of Examples and Comparative Examples were treated at 100 μl for each concentration.
양성 대조군(Positive control)은 LiCl 10 mM 을 사용하였다 (세포 상태에 따라, 10 mM은 control 대비 150~300%, 50mM은 control 대비 1000~4000%의 활성을 보인다).As a positive control (Positive control), 10 mM of LiCl was used (depending on the cell state, 10 mM shows 150-300% of control compared to control, and 50mM shows 1000-4000% of activity compared to control).
(3)Luciferase 활성 측정(3) Luciferase activity measurement
각 Well마다 ONE-Glo™ Luciferase Assay reagent를 100 ㎕씩 넣고 3분간 상온에서 빛을 차단하고 반응시켰다.For each well, 100 µL of ONE-Glo ™ Luciferase Assay reagent was added and the light was blocked and reacted for 3 minutes at room temperature.
Wallac Victor2 multilabel counter를 이용해서 활성을 측정하였다.Activity was measured using a Wallac Victor2 multilabel counter.
(4) 데이처 처리 방법(4) Data processing method
물질 처리를 하지 않은 대조군의 흡광도를 기준으로 하여, 대조군 대비 물질 처리군의 luminescence 세기를 % 값으로 환산하여 얻은 수치를 Response ratio(%)로 하였다. 상기 Response ratio 수치가 클수록 Wnt 신호 전달 활성이 우수한 것을 의미한다.Based on the absorbance of the control group without material treatment, the value obtained by converting the luminescence intensity of the material treatment group to the control group as a percentage value was taken as a response ratio (%). The larger the response ratio, the better the Wnt signal transduction activity.
도 1은 실시예 및 비교예에서 각각 제조된 조성물의 Wnt 신호 전달 활성을 나타낸 그래프이다.1 is a graph showing Wnt signal transduction activity of compositions prepared in Examples and Comparative Examples, respectively.
도 1을 참조하면, 실시예 1의 아피게닌과 카페인의 조합, 실시예 2의 아피게닌과 인삼 추출물의 조합, 실시예 3의 아피게닌과 녹차 추출물의 조합을 포함하는 조성물이 Wnt 신호 전달 활성이 우수한 것을 알 수 있다.Referring to FIG. 1, a composition comprising a combination of apigenin and caffeine in Example 1, a combination of apigenin and ginseng extract in Example 2, and a combination of apigenin and green tea extract in Example 3 has Wnt signal transduction activity. You can see that it is excellent.
반면, 비교예 3 내지 7과 같이, 아피게닌, 카페인, 인삼 추출물, 녹차 추출물 및 마데카소사이드(madecassoside)를 각각 단독으로 포함하는 조성물과 비교예 8과 같이 아피게닌과 마데카소사이드의 조합을 포함하는 조성물은 Wnt 신호 전달 활성이 실시예 1,2,3에 비해 현저히 저하되는 것을 알 수 있다.On the other hand, as in Comparative Examples 3 to 7, the composition comprising apigenin, caffeine, ginseng extract, green tea extract, and madecassoside alone, and a combination of apigenin and madecassoside as in Comparative Example 8 The composition to be found that Wnt signal transduction activity is significantly reduced compared to Examples 1,2,3.
이로부터, 실시예 1의 아피게닌과 카페인의 조합, 실시예 2의 아피게닌과 인삼 추출물의 조합, 실시예 3의 아피게닌과 녹차 추출물의 조합을 포함하는 조성물은 각 성분의 조합에 의한 시너지 작용으로 인하여 Wnt 신호 전달 효능이 크게 개선되는 것을 확인할 수 있었다.From this, the composition comprising a combination of apigenin and caffeine in Example 1, a combination of apigenin and ginseng extract in Example 2, and a combination of apigenin and green tea extract in Example 3, synergistic action by combination of each component As a result, it was confirmed that the efficiency of Wnt signaling was greatly improved.
이상에서 본 발명은 비록 한정된 실시예와 도면에 의해 설명되었으나, 본 발명은 이것에 의해 한정되지 않으며, 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자에 의해 본 발명의 기술사상과 아래에 기재될 특허청구범위의 균등범위 내에서 다양한 수정 및 변형이 가능함은 물론이다.Although the present invention has been described above by way of limited embodiments and drawings, the present invention is not limited by this, and is described below by the person skilled in the art to which the present invention pertains, and the technical idea of the present invention. Of course, various modifications and variations are possible within the scope of the equivalent claims.
Claims (9)
상기 아피게닌은 상기 조성물 전체 중량을 기준으로 40 내지 60 중량%로 포함되는 것인, 피부 재생 및 육모 촉진용 조성물.According to claim 1,
The apigenin is contained in 40 to 60% by weight based on the total weight of the composition, the composition for promoting skin regeneration and hair growth.
상기 인삼 추출물 및 녹차추출물로 이루어진 군에서 선택되는 1종 이상은 상기 피부 재생용 조성물 전체 중량을 기준으로 40 내지 60 중량%로 포함되는 것인, 피부 재생 및 육모 촉진용 조성물.According to claim 1,
At least one selected from the group consisting of the ginseng extract and green tea extract is contained in 40 to 60% by weight based on the total weight of the composition for skin regeneration, the composition for promoting skin regeneration and hair growth.
상기 조성물은 윈트/베타-카테닌 신호(Wnt/beta-catenin signal)를 활성화하는 것인, 피부 재생 및 육모 촉진용 조성물.According to claim 1,
The composition is to activate the win / beta-catenin signal, a composition for promoting skin regeneration and hair growth.
상기 조성물의 농도는 고형분 농도를 기준으로 10 내지 40 ppm인 것인, 피부 재생 및 육모 촉진용 조성물.According to claim 1,
The concentration of the composition is 10 to 40 ppm based on the solid content concentration, the composition for promoting skin regeneration and hair growth.
상기 조성물은 아피게닌 및 카페인을 포함하는 것인, 피부 재생 및 육모 촉진용 조성물.According to claim 1,
The composition will include apigenin and caffeine, skin regeneration and hair growth promoting composition.
상기 조성물은 아피게닌 및 인삼 추출물을 포함하는 것인, 피부 재생 및 육모 촉진용 조성물.According to claim 1,
The composition comprises apigenin and ginseng extract, a composition for promoting skin regeneration and hair growth.
상기 조성물은 아피게닌 및 녹차 추출물을 포함하는 것인, 피부 재생 및 육모 촉진용 조성물.According to claim 1,
The composition will contain apigenin and green tea extract, a composition for promoting skin regeneration and hair growth.
상기 조성물은 화장료 조성물, 식품 조성물 및 약학 조성물로 이루어진 군에서 선택되는 1종 이상인, 피부 재생 및 육모 촉진용 조성물.
According to claim 1,
The composition is at least one selected from the group consisting of cosmetic composition, food composition and pharmaceutical composition, a composition for promoting skin regeneration and hair growth.
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