KR20180047227A - Composition for Preventing Hair Loss or Improving Hair Growth Containing Extract of Yellow-Colored Leaf of Soybean - Google Patents

Abstract

The present invention relates to a composition for preventing hair loss or improving hair growth containing an extract of autumn soybean leaves, and more specifically, to a composition having an effect of preventing hair loss or improving hair growth by containing an extract of autumn soybean leaves, wherein the extract is extracted from bean leaves in which the color of bean leaves has changed to yellow during the growth of beans. When the composition containing the extract of autumn soybean leaves according to the present invention is used, the hair loss is prevented or the hair growth is improved by promoting the activity of a Wnt/β-catenin signal transduction system in tissue associated with hair loss such as hair paper cells.

Description

TECHNICAL FIELD The present invention relates to a composition for preventing hair loss or improving hair growth,

The present invention relates to a composition for preventing hair loss or hair growth, which contains an extract of Extract of Green Tea Extract, and more specifically, a composition for preventing hair loss or improving hair growth by containing an extract extracted from a soybean leaf whose color changes to yellow during soybean growth ≪ / RTI >

The scalp is composed of the epidermis, dermis and subcutaneous tissue, and the outermost tissue, the epidermis, protects the sensitive tissues of the body from damage. However, environmental factors affecting the scalp may result in loss of scalp function due to stress and lack of influence due to external stimuli such as temperature changes, humidity changes, exposure to pollutants, etc., and may also affect hair loss .

The tissues associated with hair loss include sebaceous glands, keratinocytes of epidermis and hair follicles, dermal papilla cells, dermal papilla cells, sweat glands, root sheath of the scalp hair follicle).

It is known that dihydrotestosterone (DHT) is involved in the tissues in the expression of male transcripts, skeletal muscle increase and spermatogenesis involve testosterone, acne, sebum increase, hair loss and enlargement of the prostate Thiboutot et al., J. Invest. Dermatol ., 105 (2): 209-14, 1995).

Male alopecia is caused by a male hormone called testosterone. When this testosterone is converted to a more powerful hormone, dihydrotestosterone, by an enzyme called 5-alpha reductase (5-alpha reductase) Acting on the hair follicle, leads the hair follicle from the growing stage to the regenerator stage to cause hair loss. Therefore, a method of inhibiting the production of DHT by 5 alpha-reductase in order to treat male alopecia is mainly used.

On the other hand, finasteride, a 5-alpha reductase inhibitor, has been shown to reduce hair loss by decreasing hair loss hormone DHT in patients with hair loss (Van Neste et al. , 2000) A major target for hair loss treatment is the research on finding a pharmacologically active substance for the treatment of alopecia by inhibiting the activity of 5-alpha reductase, a biosynthetic enzyme involved in the reaction of dihydrotestosterone in testosterone, Many are being done.

Female alopecia are caused mainly by a decrease in the amount of estrogen after menopause. Minoxidil or estrogen is mainly used as a treatment for alopecia areata. Alopecia areata is caused by autoimmune diseases, mental stress, genetic predisposition. These alopecia areata are fundamentally different from those of androgenetic alopecia, and different methods of treatment are used, such as a method of treating corticosteroids, minoxidil being applied to the affected part or artificially induced to the affected part.

As a result, there have been no commercially available products for the purpose of promoting blood circulation, inhibiting male hormone function, and enhancing hair follicle function. However, . For example, minoxidil has been reported to cause sticky sensation and side effects that cause irritation to the skin. Pinastaride is currently used as a preparation for oral administration, but adverse effects such as sexual dysfunction have been reported depending on its consumption, There was a lot of inconvenience to use because it was expected to be effective only by oral administration.

For prevention and treatment of such hair loss, hormone replacement therapy is used as the most effective method. However, when a hormone such as estrogen is administered for a long time, there is a risk of carcinogenesis such as breast cancer and uterine cancer, and side effects such as headache and weight increase occur.

On the other hand, soybean isoflavone, which is a phytoestrogen extracted from plants, has been widely used for the purpose of alleviating hair loss, but the issue of effectiveness and safety has been continuously raised. Therefore, it is urgently required to develop a therapeutic agent for alopecia using a more safe and effective natural-derived substance.

Selective estrogen receptor modulators (SERMs) are selective estrogen receptor modulators that bind to estrogen receptors and act as estrogen agonists in some tissues and act as estrogen antagonists in other tissues. Using the characteristics of SERM, SERM-containing drugs are being developed which are safe in tissues such as uterus and breast which are highly likely to be carcinogenic, and are effective in improving menopausal syndrome.

Apigenin is a vegetable polyphenol that is widely distributed in fruits and vegetables, and is particularly abundant in parsley, celery, garlic, and cabbage, and is known to have characteristics such as anti-inflammatory, anti-cancer, and free radical elimination Kim et al., Prostag . Leukotr . Ess . , 58: 17-24, 1998).

Coumestrol is known to be present in trace amounts or rarely in beans or legume plants. Compared with isoflavones, the estrogenic activity of cumestrol is 30 to 100 times higher than that of isoflavones and is very useful in relieving cervical, bone marrow, breast or brain cancer, and menopausal symptoms.

Caffeine promotes hair follicle growth by stimulating the metabolism of dermal papilla cells (Kren et al, Zygote, 12 (1): 31-38, 2004; Tsianakasa et al., Arch. Dermatol Res. , 2005) and inhibit the action of dihydrotestosterone, an active male hormone that inactivates hair follicles (Fischer et al., Int J Dermatol. , 46 (1): 27-35, 2007). However, since too much caffeine can affect the dermal papilla cells and necrotize the cells (Hashimoto et al., Cancer Res. , 64 (9): 3344-3349, 2004) There are also research results. In other words, if caffeine reaches hair follicles in an amount sufficient to prevent hair loss and hair growth, drink 60 cups of caffeinated coffee or tea a day, or if caffeine solution can be applied to scalp, And thus it has been difficult to develop a safe and effective anti-hair-loss agent.

Ginseng ( Panax ginseng CA Mey. 1909) is a perennial plant belonging to Araliaceae. It is used for medicinal purposes. It is said to supplement the vitals by watching the five ovaries. The efficacy of ginseng on the active ingredient has been revealed by Brekhman (1957) as a study of ginsenoside or saponin. Ginsenosides are known to have many effects such as cancer cell proliferation inhibition, hypertension improvement, antioxidant effect, and anti-stress.

Green tea has been drinking from the Three Kingdoms period as a perennial evergreen plant belonging to the genus Camellia (for example, Camellia sinensis ), and recently its value has been re-recognized as the functionalities of the various ingredients in green tea gradually become known have. It has been found that green tea contains a large amount of useful components such as polyphenols (e.g., catechins), caffeine, amino acids and vitamin C, and thus has excellent effects on hangover and nicotine detoxification, fatigue recovery, The antioxidant activity, anticholesterol effect, hypoglycemic action, antitumor action and platelet aggregation inhibitory action of the most abundant polyphenols are proved and their functionality is attracting much attention.

Catechin, which is a major physiologically active substance of green tea, is a kind of polyphenol. It contains about 100 mg in a cup of green tea. Epigallocatechin gallate (hereinafter referred to as "EGCG") and epicatechin gallate (Hereinafter referred to as "GC") and epicatechin (hereinafter referred to as "EC") and epigallocatechin (hereinafter referred to as "EGC"), Quot;). These components can be used as food materials having various biological control functions such as anti-cavitation, antibacterial, antioxidant, deodorizing action, inhibition of blood glucose level and elevation of blood cholesterol, antitumor, And is widely used in the field of processed foods. These catechins are known to have antioxidative effects that are three to four times higher than those of vitamin C and vitamin E.

Catechins are water-soluble, but tea leaves also contain lipid-soluble components such as vitamin E and beta-carotene, and these components are used to remove active oxygen or oxygen-derived products that adversely affect the immune response or cancer development in vivo It is known that catechin, which is known to be involved and at the same time has antioxidant effect, can be expected to have a synergistic effect.

The Wnt / β-catenin signaling pathway promotes the formation of hair follicles (Andl T, et al ., Dev Cell, 2 (5): 643-653, 2002) (Huelsken J, et al., Cell 105 (4)), which plays an important role (Kishimoto J, et al., Genes & Dev , 14: 1181-1185, 2000), promoting differentiation from stem cells to keratinocytes : 533-545, 2001).

The activity of the Wnt /? -Catenin signaling system is known to act on skin formation and hair follicle development, and it has been known that hair growth is inhibited in a mouse knock-down of the Wnt gene. Thus, a substance promoting the activation of the Wnt / β-catenin signaling system present in hair follicle stem cells can promote hair growth.

Minoxidil is one of the hair growth promoting drugs approved by the Food and Drug Administration (FDA). It was originally developed as a vasodilator for the treatment of hypertension. However, as a side effect, hirsutism was reported and now it is used as a hair growth promoter . Although the specific mechanism of action of minoxidil on hair growth has not yet been clearly elucidated, it is believed that K _ATP -channel opening, increased nutritional supply through vasodilation and activation of the β-catenin pathway in human dermal papilla cells Are thought to induce hair growth. Human dermal papilla cells are mesenchymal-derived fibroblasts that secrete protein through secretion of proteins such as insulin-like growth factor-1 (IGF-1), hepatocyte growth factor (HGF), and fibroblast growth factor- . Minoxidil is known to have an effect of proliferating human dermal papilla cells through reduction of apoptosis of human dermal papilla cells.

Thus, measuring the 5-alpha reductase activity inhibitory effect, the NIH3T3 fibroblast proliferation effect, and the growth proliferation effect of human dermal papilla cells of any substance indicates that the substance effectively treats hair loss and improves hair growth This can be a valid indicator to assess if it is possible.

On the other hand, according to the prior art, the following Patent Document 1 discloses a skin inflammation, an allergy and an atopy improvement effect and a skin moisturizing effect of a composition containing a soybean leaf extract, and Patent Document 2 discloses a composition containing a ginseng extract and a green tea extract A composition for improving hair growth and improving hair loss symptoms, which comprises an extract derived from a canola having a yellow color of the canola during the growth of soybean, Is not disclosed.

Under these technical backgrounds, the inventors of the present invention have developed a composition showing the effect of improving hair growth and alopecia symptoms by containing an extract of an extract of the colored leaves of bean leaves, that is, an extract extracted from a canola leaf in which the color of the bean leaves has changed from yellow to yellow during the growing process Respectively.

Korean Patent Publication No. 10-2014-0034964 (March 21, 2014). Korean Patent Publication No. 10-2015-0030095 (Mar. 19, 2015).

Accordingly, the present inventors have made intensive efforts to develop a composition containing a plant-derived SERM (phytoSERM) effective for preventing hair loss or improving hair growth, and as a result, found that the extract of soybean leaves The present inventors confirmed that the extract promotes hair growth promotion and alopecia symptom improvement activity by promoting the activity of the Wnt /? - catenin signal transduction system of dermal papilla cells, and completed the present invention.

Accordingly, it is an object of the present invention to provide a composition containing an extract of an Aspenaceae leaf which is excellent in hair growth promotion and hair loss prevention effect.

In order to achieve the above object, the present invention provides a hair loss preventing or hair growth improving composition comprising an extract of Asahi Kasei as an active ingredient.

In the case of using the composition containing the leaves of the present invention, there is an effect of preventing hair loss or improving hair growth by promoting the activity of the Wnt /? - catenin signal transduction system in tissues associated with hair loss such as dermal papilla cells.

Fig. 1 is a graph showing the results of HPLC analysis of soybean leaf extract according to growth stages of soybean (soybean curd).
2 is a graph showing the results of HPLC analysis of an extract of soybean curd lees and soybean extract according to the present invention.
FIG. 3 is a graph showing the degree of the reaction according to the Wnt / β-catenin signaling cascade activity of human dermal papilla cells treated with the leaves of the present invention or the soybean extract according to the present invention.
FIG. 4 is a graph showing hair growth lengths of a human hair follicular organ treated with the leaves of the present invention or soybean extract.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In general, the nomenclature used herein is well known and commonly used in the art.

In the present specification, " Maple cane leaves " refers to a canola having a yellow color in the color of the cane during the growth of the bean, and the cane is in a state where the bean and the bean turn yellow (hereinafter referred to as R7 period); 2) the state of the bean pods and beans turning completely yellow as the leaves fall (R8 period below); It means the bean leaves in. Specifically, " maple leaves " means a green or brown bean that is in a yellow state or a fully yellowed state, and the broadest concept including the case where the bean leaves are partially turned yellow .

In the aspect of the present invention, the extract of green tea leaves can be extracted from the soybean leaves of steps R7 to R8 during the growth stage of the following soybean. In this specification, the step of growing the soybean is as follows.

VE stage: 1 ~ 2 weeks after planting the seeds, the soil is covered with cotyledon

VC stage: cotyledon blooms, a word grows on it, and a lobe is created

Step V1: A word is generated from the first lobe and three leaves are created

Step V2: One more word was created on the V1 Stage to create three leaves

Step V3: One more word is created on the V2 Stage to create three leaves

Step V4: One word is created on the V3 Stage to create three leaves

Step V5: One more word was created on the V4 Stage to create three leaves

Step R2: The state of the bean blooms

Step R4: Completion of pod creation

Step R5: Bean is produced in the pods

Step R6: Green beans in pods

Step R7: The state of the bean pods and beans turning yellow

Step R8: The state of the bean pods and beans turning yellow as the leaves fall

In one embodiment of the present invention, the change in the activity of the Wnt / beta -catenin signal transduction system of hair follicular stem cells following the treatment of the leaves of soybean curd extract was measured. As a result, as shown in Fig. 3, the degree of Wnt / [beta] -catenin luciferase reaction of 150% or more (i.e., activity of Wnt / [beta] -catenin signal transduction system ), And the degree of the reaction was increased depending on the concentration of the extract of the colored leaves of the leaves (Example 4). Therefore, it was found that the extract of the leaves of green tea leaves promotes the hair growth by promoting the activity of the Wnt /? -Catenin signal transduction system, which is a signal for promoting hair growth of hair follicle stem cells (see Test Example 2).

In another embodiment of the present invention, the hair growth effect of the human hair follicle organ according to the treatment of the leaves of the canola extract was confirmed. As a result, as shown in Fig. 4, in the concentration range of 1, 10, and 50 ppm, the extract of canola leaves (Example 4) exhibited hair growth effect equal to or higher than that of the positive control, Growth was the best. Therefore, it was confirmed that the extract of the leaves of the leaves of the present invention is effective for growing hair of a human hair follicle organ (see Test Example 3).

In another embodiment of the present invention, there is provided a method for the treatment of a mixture comprising an extract of an Aspenaceae leaf extract or an extract of Apigenin, cumestrol, caffeine, ginseng extract and green tea extract, The proliferation effect of human dermal papilla cells was confirmed. As a result, as shown in Table 2, human dermal papilla cells treated with 1, 10 or 50 ppm of the leaves of the canola extract (Example 4) in comparison with human dermal papilla cells treated with DMSO (negative control) In particular, the human dermal papilla cells treated with the leaves of the extract of canola leaves at the concentration of 10 ppm (Example 4) showed the highest cell proliferation rate, which is equivalent to that of human dermal papilla cells treated with minoxidil (positive control) Respectively. In addition, any one substance selected from the group consisting of apigenin, cumestrol, caffeine, ginseng extract (Example 5) and green tea extract (Example 6) and 10 ppm of maple leaf extract (Example 4) As a result of treating a mixture of human dermal papilla cells, the proliferation rate of human dermal papilla cells was remarkably increased. Therefore, it was confirmed that synergy effect that the effect of hair growth is further promoted when treating the human dermal papilla cells with the mixture of the leaves and the soybean curd extract and the above substances was confirmed (see Test Example 4).

The substance that promotes hair growth through regulation of the activity of the Wnt / beta -catenin signaling system is not involved in promoting hair growth through regulating male hormone and / or blood circulation, and thus inhibits male hormone or promotes blood circulation It is possible to improve severe hair loss and feminine hair loss which have not been resolved by the method.

Accordingly, in one aspect, the present invention relates to a pharmaceutical composition, a health functional food composition or a cosmetic composition for preventing hair loss or improving hair growth, which comprises an extract of Aspergillus oryzae as an active ingredient, Can be extracted from the canola leaves when the whole is changed to yellow, and more preferably, it is extracted from the canola leaves at the stages R7 to R8 during the growing stage of the soybean.

In another aspect, the present invention relates to a method of preventing or treating hair loss comprising at least one selected from the group consisting of apigenin, coumestrol, caffeine, ginseng extract, and green tea extract, Hair growth improving composition.

In the present invention, 'beans' are not limited in their kind and include, for example, Seoritae , Glycin max MERR, Seomoktae, Rhynchosia Nolubilis , Black soybean, Glycine max (L.) Merr., Blue bean, Glycime max MERR, Yellow bean, Glycime max MERR, Field bean, Vicia faba ), kidney bean (kidney bean, Phaseolus vulgaris ), pinto bean ( Phaseolus vulgaris L.), small red bean ( Vigna) angularis), reap (small black bean, Phaseolus angularis WF WIGHT.), bean sprouts (sprouting bean, Glycine max (L. ) Merr.) , and soybeans (soybean, Glycine max) be at least one selected from the group consisting of, but , But is not limited thereto.

In one aspect of the present invention, the " colored leaves soybean extract " includes all of the substances obtained by extracting the components of the colored leaves of the leaves, regardless of the extraction method, the extraction solvent, the extracted components or the form of the extract, It contains substances obtained through extraction process including heat, acid, base, enzymes, etc., and extracts the material from the leaves of the leaves and extracts them. It is a broad concept that includes all substances that can be obtained by treatment. Specifically, the processing or treatment may be a step of additionally fermenting or treating with an extract of soybean curd lees of autumn leaves. Therefore, in one aspect of the present invention, the extract of the colored leaves of the leaves may be a fermented product.

In one aspect of the present invention, the " colored leaves of the leaves " may be in the form of an extract, or may be a raw leaves of green leaves, a pulverized product of the herb itself, a dried product of herbal medicine, a dried product of herbal medicine, But is not limited to. There is no limitation on the method for obtaining the leaves of the present invention, and the present invention can be used by cultivating or purchasing commercially available soybean leaves, or a part or all of the root portions of the herbal can be used. More specifically, at least one selected from the group consisting of leaves, stems, roots, and flowers of the leaves can be used. More specifically, flowers, leaves, and stems may be used. In one aspect of the present invention, in case of maple leaves, the maple leaves are not necessarily manufactured through drying, and are not limited as long as they are in a form suitable for extracting the active ingredient of the maple leaves.

In one aspect of the present invention, the colored leaves of soybean extract may be at least one extract selected from the group consisting of water, organic solvents, and mixtures thereof.

In one aspect of the present invention, water comprises distilled water or purified water, and the organic solvent is at least one selected from the group consisting of C1-C6 lower alcohol, acetone, ether, ethyl acetate, diethyl ether, ethyl methyl ketone and chloroform But is not limited thereto. Specifically, the alcohol may be methanol or ethanol.

The term " improve " or " alleviate ", as used herein, refers to the reduction or elimination of conditions, diseases, disorders or phenotypes including abnormalities or symptoms, syndromes. Herein, improvement or alleviation of hair loss means preventing, ameliorating or alleviating hair loss diseases by promoting the activity of the Wnt / beta -catenin signal transduction system.

As used herein, " treatment " means any action that alleviates or alleviates symptoms of hair loss disease, and more particularly, refers to any disease or condition to which the term applies, or to reversal of one or more symptoms of the disease or condition Or ameliorating, inhibiting, or preventing the progress of the disease.

The extract of the leaves of the present invention may be characterized by containing a phyto-selective estrogen receptor modulator (phyto-SERM). phyto-SERM is a combination of "phyto" meaning "plant-derived" and "selective estrogen receptor modulator (SERM)". A selective estrogen receptor modulator refers to a substance that binds to an estrogen receptor and acts as an estrogen agonist in some tissues and as an estrogen antagonist in other tissues. The composition containing the extract of canola leaves of the present invention may act as an antagonist to the estrogen receptors in tissues such as uterus and breast which are highly likely to be carcinogenic to reduce the risk of cancer such as uterine cancer and breast cancer, Is expected to contain phyto-SERM, which acts as an agonist in human dermal papilla cells and is effective in promoting hair growth and preventing hair loss.

According to the present invention, the extract of the colored leaves may be contained in an amount of 1 to 60% by weight, preferably 5 to 50% by weight based on the total weight of the composition. When the content of the colored leaves is less than 1% by weight, it is difficult to expect hair growth promotion and hair loss prevention effect. When the content exceeds 60% by weight, the stability of the formulation is lowered and the stability of the extract is also affected, It is expected.

In the present invention, the composition may further include at least one selected from the group consisting of apigenin, coumestrol, caffeine, ginseng extract, and green tea extract.

When the composition contains a mixture composed of an extract of green tea leaves and apigenin, the mixture is preferably a mixture of green tea leaf extract and apigenin in a weight ratio of 1 to 10: 1 to 10, preferably 1: 2: 1 to 2 . ≪ / RTI >

When the composition contains a mixture composed of an extract of an Aspergillus ficus-indica extract and a mixture of cumestrol, the mixture is preferably a mixture of an extract of an Aspergillus oryzae and a derivative of cumestrol in a weight ratio of 1 to 10: 1 to 10, preferably 1 to 2: And mixed.

When the composition contains a mixture composed of an extract of green tea leaves and caffeine, the mixture is characterized in that the extract of green tea leaves and caffeine are mixed at a weight ratio of 1 to 10: 1 to 10, preferably 1: 2: 1 to 2 .

When the composition contains a mixture composed of an extract of green tea leaves and a ginseng extract, the mixture is preferably a mixture of green tea leaves and ginseng extract at a weight ratio of 1 to 10: 1 to 10, preferably 1: 2: 1 to 2 . ≪ / RTI >

When the composition contains a mixture composed of an extract of green tea leaves and green tea extract, the mixture is preferably a mixture of green tea leaves and green tea extract at a weight ratio of 1 to 10: 1 to 10, preferably 1: 2: 1 to 2 . ≪ / RTI >

In the present invention, the canola extract may promote the activity of the Wnt / beta -catenin signal transduction system, preferably promotes the activity of the Wnt / beta -catenin signal transduction system of the dermal papilla cell, More preferably promotes the activity of the Wnt / beta -catenin signaling system of human dermal papilla cells.

In the present invention, the soybean-leaf extract may be characterized by growing hair of a hair follicle organ.

In the present invention, the bean leaf extract may be characterized in that it promotes hair growth and / or alleviation of hair loss symptoms by promoting the activity of the Wnt / beta -catenin signal transduction system of the dermal papilla cells.

The pharmaceutical composition containing the autumn colored leave leaf extract of the present invention can be used as a preservative, a stabilizer, a wetting agent or an emulsifying accelerator, a pharmaceutical adjuvant such as a salt and / or a buffer for controlling osmotic pressure and other therapeutically useful substances (carrier, excipient, ), And may be formulated into various oral administration form or parenteral administration form according to a conventional method.

The term " carrier " is defined as a compound that facilitates the addition of a compound into a cell or tissue. For example, dimethylsulfoxide (DMSO) is a commonly used carrier that facilitates the introduction of many organic compounds into cells or tissues of an organism.

The term " excipient " refers to a substance which, when used in tablets or pills, is added to the medicine to make it easier to eat or to have a certain color and shape when the amount of the drug is low. Lactose or starch is mainly used do.

The term " diluent " is defined as a compound that not only stabilizes the biologically active form of the compound of interest, but also dilutes in water to which the compound is dissolved. Salts dissolved in buffer solutions are used as diluents in the art. A commonly used buffer solution is phosphate buffered saline, since it mimics the salt state of the human solution. Since buffer salts can control the pH of the solution at low concentrations, buffer diluents rarely modify the biological activity of the compounds.

The composition containing the colored leaves soybean extract used herein can be administered to a human patient as such, or as a pharmaceutical composition mixed with other active ingredients, such as in a combination therapy, or together with a suitable carrier or excipient.

Examples of carriers, excipients and diluents that can be contained in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose , Methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

Examples of the oral administration agent include tablets, pills, hard and soft capsules, liquids, suspensions, emulsions, syrups, powders, powders, granules, granules and pellets, , Diluents (such as lactose, dextrose, sucrose, mannitol, sorbitol, cellulose and glycine), lubricants (such as silica, talc, stearic acid and magnesium or calcium salts thereof and polyethylene glycols) . Tablets may also contain binders such as magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose and polyvinylpyrrolidine, optionally mixed with starch, agar, alginic acid or its sodium salt Such as disintegrants, absorbents, coloring agents, flavoring agents, and sweetening agents. The tablets may be prepared by conventional mixing, granulating or coating methods.

The parenteral administration may be, for example, an injection, a drip, an ointment, a lotion, a gel, a cream, a spray, a suspension, an emulsion, a suppository, It is not.

The pharmaceutical composition according to an embodiment of the present invention may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously, and the like. The pharmaceutical composition according to one embodiment of the present invention may be administered topically, for example, to the scalp.

In addition, the pharmaceutically acceptable dose, that is, the dosage of the active ingredient may be determined according to the age, sex, and weight of the subject to be treated, the specific disease or condition to be treated, the severity of the disease or condition, It will be different. Dosage determinations based on these factors are within the level of those skilled in the art. The dosage is, for example, from 1 to several times to be administered in an amount of 0.01 to 5000 mg, preferably 0.1 to 2000 mg, more preferably 0.5 to 500 mg, and most preferably 1 to 100 mg per kg of body weight per day , But the dose is not limited to the scope of the present invention by any means.

Since the extract of the canola leaves or the leaves of the present invention is a natural substance, there is no toxicity, and thus it can be continuously and widely used as a medicine.

Pharmaceutical compositions suitable for use in the present invention include compositions in which the active ingredients, including the colored leaves soybean extract, are contained in an amount effective to achieve its intended purpose. More specifically, a therapeutically effective amount refers to an amount of a compound that is effective to prolong the survival of an object to be treated or to prevent, alleviate, or alleviate symptoms of the disease. The determination of a therapeutically effective amount is well within the ability of those skilled in the art, particularly in light of the detailed disclosure provided herein.

The therapeutically effective amount of the maple bean leaf extract used in the methods of the present invention and the composition (compounds) containing it as an active ingredient can be measured early from the cell culture analysis. For example, a dose can be calculated in an animal model to obtain a circulating concentration range that includes IC ₅₀ (half maximal inhibitory concentration) or EC ₅₀ (half maximal effective concentration) determined in cell culture. Such information can be used to more accurately determine useful doses in humans.

This leaves kongip extract as set forth in, or Toxicity and therapeutic effectiveness of this composition (compounds) which contains as an active ingredient, for example, LD ₅₀ (lethal dose for 50% of the population), ED ₅₀ (50 in the cluster %), IC ₅₀ (dose with therapeutic inhibition effect for 50% of the population), can be estimated by cell culture or by surface restriction procedures in laboratory animals. The dose ratio between toxic and therapeutic effects is the therapeutic index and can be expressed as the ratio between LD ₅₀ and ED ₅₀ (or IC ₅₀ ). Compounds with a high therapeutic index are preferred. Data from these cell culture assays can be used to estimate the range of doses used in humans. The dosage or amount of such compounds is preferably within the range of circulating concentrations, including the ED ₅₀ (or IC ₅₀ ), with no or little toxicity.

In the present invention, the term "functional food" or "functional food" means a food having improved functionality of a general food by adding the extract of the present invention to the ordinary food.

When the extract of the present invention is added to a general food, the physical properties and physiological functions of the general food will be improved, and the present invention can be applied to food of such improved function as a comprehensive functional food (Health functional food) 'or' functional food (health functional food) '.

In the present invention, the term "health functional food" refers to foods prepared and processed using raw materials and ingredients having useful functions in accordance with the Health Functional Food Act No. 10219, and the nutrients Or to obtain a beneficial effect in health use such as physiological action.

The health functional food containing the autumn colored cane leaf extract of the present invention may contain other ingredients or the like which can give synergy to the main effect within a range not impairing the intended main effect of the present invention. For example, additives such as perfume, coloring agent, bactericide, antioxidant, preservative, moisturizing agent, thickening agent, inorganic salt, emulsifier and synthetic polymer substance may be further added for improvement of physical properties. In addition, it may further contain auxiliary components such as water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides and seaweed extract. The above components may be mixed and selected without difficulty by those skilled in the art depending on the purpose of formulation or use, and the amount thereof may be selected within a range that does not impair the objects and effects of the present invention. In addition, the composition of the present invention itself can be used in the form of an additive for other foods.

The formulation of the health functional food containing the colored leaves of the present invention is not particularly limited. For example, it may be formulated into various forms such as tablets, granules, drinks, beverages, solutions, emulsions, . In addition, when the health functional food is administered, it may be administered by various methods such as simple drinking, injection administration, spraying or squeezing.

According to one embodiment of the present invention, the composition of the present invention can be variously applied to hair cosmetics which are formulated into a cosmetic formulation and have hair growth promoting effect.

The formulation of the cosmetic composition containing the autumn colored can-and-leaves extract of the present invention is not particularly limited and may be appropriately selected according to the purpose. For example, it can be applied to softeners (skin lotion and milk lotion), nutrition lotion, essence, nutrition cream, massage cream, pack, gel, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, Body Lotion, Body Cream, Body Oil, Body Essence, Hair Tonic, Hair Conditioner, Hair Essence, Hair Lotion, Hair Nourishing Lotion, Hair Shampoo, Hair Rinse, Hair Treatment, Hair Cream, Hair Cream, Hair Moisture Cream, Hair Hair cream, hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair drying agent, hair preservative, hair dye, hair wave agent, hair bleaching agent, hair gel, hair glaze, hair dresser , Hair lacquers, hair moisturizers, hair mousses, and hair sprays, but the present invention is not limited thereto, Hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream, hair moisturizing cream, hair massage cream, hair wax, hair aerosol, Hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair dyes, hair moisturizers, Mousse or hair spray.

When the composition of the present invention is formulated into a cosmetic formulation, the composition of the present invention may contain all the ingredients commonly used in cosmetics in addition to the above-mentioned extracts. Examples of the composition include water-soluble vitamins, oil-soluble vitamins, polymer peptides, Lipids, and seaweed extracts.

The polymeric peptide may be selected from any of those that can be compounded in the cosmetic, and preferably collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin and the like can be selected. The polymeric peptide can be obtained by a conventional method such as purification from a culture broth of a microorganism, an enzymatic method, or a chemical synthesis method, or it can be purified from natural products such as ducks such as pigs and cows and silk fiber of silkworms.

The polymeric polysaccharide may be selected from any of those that can be compounded in the cosmetic, and preferably hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be selected. For example, chondroitin sulfate or a salt thereof can be usually purified from mammals or fish.

Sphingo lipids may be selected from any of those that can be incorporated in cosmetics, but ceramides, phytosphingosine, sphingoglycolipids and the like may be preferably selected. Sphingoid lipids can be purified from ordinary mammals, fish, shellfish, yeast or plants by conventional methods or can be obtained by chemical synthesis.

The cosmetic of the present invention may contain, in addition to the above-mentioned essential ingredients, other ingredients usually added to cosmetics, if necessary. In this case, the compounding ingredient may be a preservative, a moisturizer, an emollient, a surfactant, an organic or inorganic pigment, an organic powder, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a plant extract, a pH adjuster, , A cold agent, an antiperspirant agent, purified water, etc. may be used.

Any of the above components may be blended within the range not impairing the object and effect of the present invention, but it is preferably 0.001 to 50% by weight based on the total weight, 0.01 to 5% by weight, more preferably 0.01 to 3% by weight.

The ingredients contained in the composition formulated in the cosmetic formulations of the present invention may contain, as an active ingredient, the ingredients commonly used in cosmetics in addition to the above extracts, for example, stabilizers, solubilizers, vitamins, And the like.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for illustrating the present invention and that the scope of the present invention is not construed as being limited by these embodiments. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

[ Example  1 ~ 4] Production of Soybean Leaf Extract by Growth Stage

(V5 level) after 45 days of sowing, cannellal leaves (78 days) from R2 to 3 stage (V5 level) after sowing of Glycine max (L.) Merrill (National seed source varieties registration No. 01-0003-134) ), The canola leaves (stage R6) aged 107 days, and the canola leaves (stage R8) aged 139 days were each washed with purified water, and then dried and chalcogenized. 100 g of each of the hemihydrated cane leaf powder was added to 1 liter of an ethanol aqueous solution and extracted at room temperature (25 DEG C) for 24 hours, followed by filtration through a 300-mesh filter cloth. The filtrate was then concentrated and powdered. Thus, the powder of the bean leaf extract of Example 5 (Example 1), the bean leaf extract of Examples 2 and 3 (Example 2), the bean leaf extract of Example 6 (Example 3), and the bean leaf extract of Example 8 10.3 g each, respectively.

[ Example  5] Production of ginseng extract

The raw ginseng root was sun dried or hot air dried to prepare ginseng root. 3 kg of water was added to 1 kg of the dried ginseng root material, and the mixture was refluxed, filtered and concentrated under reduced pressure at 40 to 45 ° C to obtain 300 g of ginseng root extract.

[ Example  6] Preparation of green tea extract

4.5 L of water heated to 85 to 95 캜 was added to 300 g of green tea, and the mixture was treated for 2 to 3 hours while maintaining the above temperature. Then, the mixture was filtered, and 82.7 g of green tea extract was obtained under reduced pressure at 65 to 75 캜 .

[ Comparative Example ] Preparation of soybean extract

Xinhua bean, which is the same kind of soybeans used in the production of Example 1-4, was washed with purified water, dried and then chopped. 100 g of the hemihydrate soybean powder was added to 1 liter of an aqueous ethanol solution and extracted at room temperature (25 DEG C) for 24 hours, followed by filtration through a 300-mesh filter cloth. The filtrate was then concentrated and powdered. Thus, 10.3 g of a powder of the soybean extract was obtained.

[ Test Example  1] Comparative Analysis of Components of Soybean Leaf Extract by Growth Stage

Ingredients of the soybean leaf extract (Examples 1 to 4) and soybean extract (Comparative Example) for each stage of growth were analyzed. Each of the extracts was dissolved in 70% ethanol to prepare a 10,000 ppm solution. The components were analyzed (Waters 2996 PDA detector) using HPLC (Waters 2695 model). The stationary phase was a Mightysil RP-18 GP 250-4.6 (5 [mu] m) column from Kanto Chemical, and the mobile phase used was the composition ratio shown in Table 1 below.

Time (minutes) water Acetonitrile 0 95 5 65 62 38 70 38 62 75 95 5 80 95 5

As a result, as shown in the HPLC analysis results of FIG. 1, the extract of canola (V5) (Example 1), which had passed 45 days after sowing, in the canola leaves (R8 stage) , A new peak not found in the canola leaves (R2 ~ 3) extract (Example 2) after 78 days of sowing (Example 2) and in the canola leaf (R6) extract after 3 days of sowing (Example 3) Could know. Therefore, it was confirmed that the composition of the bean leaf extract was changed according to the growth stage of the bean leaf.

As shown in the results of the HPLC analysis of FIG. 2, the soybean leaves (R8 stage) extract (Example 4) which had passed 139 days after sowing had a higher level of isoflavone and its derivatives (Daidzein, Genistein, Daidzin, Genistin, Malonyl-Daidzin, and Malonyl-Genistin) were significantly lower and the contents of cumestrol and other unknown components were high.

[ Test Example  2] Leaf hair follicle stem cell Wnt / β- Catechin  Changes in signal transduction system activity

NIH3T3 (Wnt) cells, a follicular stem cell expressing luciferase, a reporter protein capable of measuring the activity of Wnt /? -Catenin signal transduction system, were seeded in 96 well plates (Nunc, Wiesbaden, Germany) at 10000 cells / well After seeding, the cells were cultured for 24 hours at 37 ° C in 5% CO ₂ . (Example 4) was treated at a concentration of 1, 10 or 50 ppm and then cultured for 24 hours (negative control: DMSO (Dimethyl Sulfoxide); positive control: 50 mM Lithium chloride (LiCl) of Comparative Example: common cane leaf extract). Then, 100 μL of ONE-Glo reagent (Promega, Madison, WI, USA) was added to each well of a 96-well plate and reacted for 3 minutes. After the reaction, NIH3T3 (Wnt /? -Catenin luciferase assay) of Wnt /? - catenin luciferase reaction (activity of Wnt /? - catenin signaling system) of Wnt cells.

As a result, as shown in Fig. 3, the degree of Wnt / [beta] -catenin luciferase reaction of 150% or more (i.e., activity of Wnt / [beta] -catenin signal transduction system ), And the degree of the reaction was increased depending on the concentration of the extract of the colored leaves (Example 4). On the other hand, the common soybean leaf extract, which is a comparative example, hardly showed the Wnt /? -Catenin luciferase reaction. (Generally, when the Wnt /? - luciferase reaction degree exceeds 150%, the hair growth promoting effect is judged to be effective.

Therefore, it was found that the extract of the leaves of soya leaves promotes hair growth by promoting the activity of the Wnt / β-catenin signal transduction system, which is a signal promoting hair growth of hair follicle stem cells.

[ Test Example  3] Hair growth effect of human hair follicle organ according to treatment of autumn leaves

After the hair transplantation, the human hair follicle organ was isolated from the remaining tissues one by one and cultured in William 'E medium (L-glutamine (2 mM), insulin (10 μg / ml), hydrocortisone (40 ng / ml), antibiotics (1% w / v) containing medium). The length of the hair grown from the hair follicular organ was measured at 3 days and 6 days after cultivation of the leaves (Example 4) at a concentration of 1, 10 or 50 ppm (negative control: DMSO; positive control: 50 μM minoxidil) .

As a result, as shown in Fig. 4, in the concentration range of 1, 10, and 50 ppm, the extract of canola leaves (Example 4) exhibited hair growth effect equal to or higher than that of the positive control, Growth was the best.

Therefore, it has been confirmed that the extract of the leaves of the canola leaves is effective for the hair growth of the human hair follicle organ.

[ Test Example  4] Effect of Extract of Green Leaves on Extracts and Their Mixtures on Human Dermal Pupa

Human dermal papilla cells were seeded in 96-well plates at 5000 cells / well and cultured for 24 hours at 5% CO ₂ and 37 ° C. (I) (Sigma, USA), cumesist (Sigma, USA), caffeine (Sigma, USA), or the like) (Example 4: 10 ppm) at a concentration of 5 ppm was treated with human dermal papilla cells (Sigma, USA), ginseng extract (Example 5) or green tea extract (Example 6) , And cultured for 72 hours. Cell proliferation was measured by the MTT assay (3- (4,5-Dimethyl-2-thiazolyl) -2,5-diphenyl-2H-tetrazolium bromide tetrazolium reduction assay) Respectively.

sample Cell proliferation (%) Standard Deviation(%) 0.1% DMSO (negative control) 100.0 3.4 50 [mu] M minoxidil (positive control) 112.4 9.0 1ppm Leaves of Canola Extract ( Example  4) 105.7 4.5 10ppm Leaves of Canola Extract ( Example  4) 114.1 3.6 50ppm Leaves of Canola Extract ( Example  4) 107.9 11.4 5ppm Apigenin 112.2 5.8 5 ppm cumestrol 109.6 4.4 5 ppm caffeine 127.6 2.5 5 ppm ginseng extract (Example 5) 124.9 8.7 5 ppm green tea extract (Example 6) 106.5 5.1 10ppm Green bean leaf extract ( Example 4) + 5ppm Apigenin 131.1 6.4 10ppm Leafy-green bean leaf extract ( Example 4) + 5ppm Cumestrol 129.3 5.5 10ppm Green bean leaf extract ( Example 4) + 5ppm Caffeine 138.8 4.7 10ppm Green bean leaf extract ( Example 4) + 5ppm Ginseng extract (Example 5) 140.2 10.4 10 ppm Green bean leaf extract ( Example 4) + 5 ppm Green tea extract (Example 6) 133.9 8.3

As a result, as shown in Table 2, human dermal papilla cells treated with 1, 10 or 50 ppm of the leaves of the canola extract (Example 4) in comparison with human dermal papilla cells treated with DMSO (negative control) In particular, the human dermal papilla cells treated with the 10 ppm carnauba bean leaf extract (Example 4) showed the highest cell proliferation rate, and it was confirmed that the cell proliferation effect was equivalent to that of human dermal papilla cells treated with minoxidil (positive control) Respectively.

Meanwhile, treatment of human dermal papilla cells with 5 ppm of apigenin, coumestrol, caffeine, ginseng extract (Example 5) or green tea extract (Example 6) increased cell proliferation rate,

(Example 4) was mixed with 10 ppm of any one substance selected from the group consisting of apigenin, cumestrol, caffeine, ginseng extract (Example 5) and green tea extract (Example 6) As a result of treatment with dermal papilla cells, the proliferation rate of human dermal papilla cells was remarkably increased.

Therefore, it was confirmed that synergistic effect of further promoting the hair growth effect is obtained when human hair dough cells are treated with the mixture of the extract of green tea leaves and the above-mentioned materials.

The pharmaceutical, food and cosmetic composition containing the extract of the present invention as an active ingredient can be manufactured into various formulations (medicines, foods, cosmetics) of Formulation Examples 1 to 10, but the present invention is not limited thereto. It can be applied to various formulations and formulations including an appropriate amount of colored leaves of soybean leaf extract in consideration of functionality, cost and other conditions.

[ Formulation example  1] Preparation of soft capsules

The soft capsule filling liquid was prepared by mixing 80 mg of the extract of the canola with leaves, 9 mg of vitamin E, 9 mg of vitamin C, 2 mg of palm oil, 8 mg of vegetable hardening oil, 4 mg of yellow radish and 9 mg of lecithin and mixing them according to a conventional method. 400 mg per one capsule was filled to prepare a soft capsule. Separately from the above, a soft capsule sheet was prepared in a ratio of 66 parts by weight of gelatin, 24 parts by weight of glycerin and 10 parts by weight of sorbitol, and filled with the filling solution to prepare a soft capsule containing 400 mg of the composition of the present invention.

[ Formulation example  2] Preparation of tablets

Vitamin E 9 mg, galactooligosaccharide 200 mg, lactose 60 mg, and maltose 140 mg were mixed and granulated using a fluidized bed dryer, and 6 mg of sugar ester was added. 504 mg of these compositions were tabletted by a conventional method to prepare tablets.

[ Formulation example  3] Manufacture of Drinks

Vitamin E 9 mg, glucose 10 g, citric acid 0.6 g, and liquid oligosaccharide 25 g were mixed, and 300 ml of purified water was added to each bottle to fill 200 ml of each bottle. And then sterilized at 130 DEG C for 4 to 5 seconds to prepare a beverage.

[ Formulation example  4] Preparation of granules

The extracts were prepared by mixing 80 mg of Extract of Green Bean Leaf, 9 mg of Vitamin E, 9 mg of Vitamin C, 250 mg of anhydrous crystalline glucose and 550 mg of starch, forming into granules using a fluidized bed granulator and filling the pellets.

[ Formulation Example  5] Nourishing lotion ( Milk lotion )

The ingredients of Table 3 containing the oil component and the water component were mixed to prepare a nutritional lotion containing the autumn colored cane leaf extract of the present invention.

Compounding ingredient Content (% by weight) Purified water Balance Maple leaf extract 0.1 Wax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.5 Liquid paraffin 0.5 Montana 202 (Manufacturer: Seppic) 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount

[ Formulation Example  6] Manufacture of massage cream

The ingredients of Table 4, including the oil component and the water component, were mixed to produce a massage cream containing the leaves of the present invention.

Compounding ingredient Content (% by weight) Purified water Balance Maple leaf extract 0.1 Wax 10.0 Polysorbate 60 1.5 Piggy 60 hardened castor oil 2.0 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Montana 202 (Manufacturer: Seppic) 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount

[ Formulation Example  7] Manufacture of pack

The ingredients of Table 5 containing the oil component and the water component were mixed to prepare a pack containing the autumn colored cane leaf extract of the present invention.

Compounding ingredient Content (% by weight) Purified water Balance Maple leaf extract 0.1 Polyvinyl alcohol 13.0 Sodium carboxymethylcellulose 0.2 glycerin 5.0 Allantoin 0.1 ethanol 6.0 Pingyi-12 nonylphenyl ether 0.3 Polysorbate 60 0.3 Preservative, coloring, fragrance Suitable amount

[ Formulation Example  8] Manufacture of hair tonic

The ingredients of Table 6 containing the oil component and the water component were mixed to prepare a hair tonic containing the autumn colored bean leaf extract of the present invention.

Compounding ingredient Content (% by weight) Purified water Balance Maple leaf extract 5.0 Castor oil 5.0 Resorcinol 0.1 menthol 0.05 Panthenol 0.2 Salicylic acid 0.1 Tocopheryl acetate 0.1 Pyridoxine hydrochloride 0.1 ethanol Suitable amount Preservative, coloring, fragrance Suitable amount

[ Formulation Example  9] Hair shampoo  Produce

The ingredients of Table 7 including the oil component and the water component were mixed to prepare a hair shampoo containing the autumn colored lotus leaf extract of the present invention.

Compounding ingredient Content (% by weight) Purified water Balance Maple leaf extract 5.0 Ammonium lauryl sulfate (ALS) 20 Ammonium laureth sulfate (ALES) 20 Coca- Suitable amount Disodium iodide 0.03 Carbomer Suitable amount ethanol Suitable amount Cocamide Profile Betaine 6.0 Dimethicone (50%) 2.0 Dimethicone (70%) 2.0 DL-Panthenol Suitable amount Tocopheryl acetate Suitable amount Triethanolamine Suitable amount menthol Suitable amount Methylchloroisothiazolinone
Mixture of methylisothiazolinone (MC / IMI) 0.03 Methylparaben Suitable amount Preservative, coloring, fragrance Suitable amount

[ Formulation Example  10] Hair Conditioner  Produce

The ingredients of Table 8 containing the oil component and the water component were mixed to prepare a hair conditioner containing the autumn colored bean leaf extract of the present invention.

Compounding ingredient Content (% by weight) Purified water Balance Maple leaf extract 5.0 ethanol 3.5 Self emulsifying glycerin monostearate 1.5 Propylene glycol 2.5 Stearylmethylbenzylammonium chloride (25%) 7.0 Methyl paraoxybenzoate 0.3 Preservative, coloring, fragrance Suitable amount

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will readily appreciate that many modifications are possible in the exemplary embodiments without materially departing from the novel teachings and advantages of this invention. something to do. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (8)

A composition for preventing hair loss or improving hair growth, comprising an extract of Asahi Kasei as an active ingredient.
The composition according to claim 1, wherein the colored leaves extract is extracted from the canola leaves when part or all of the leaves are yellow.
3. The composition according to claim 2, wherein the colored leaves extract is extracted from the canola leaves of steps R7 to R8 during the growth of the following soybean.
VE stage: 1 ~ 2 weeks after planting the seeds, the soil is covered with cotyledon
VC stage: cotyledon blooms, a word grows on it, and a lobe is created
Step V1: A word is generated from the first lobe and three leaves are created
Step V2: One more word was created on the V1 Stage to create three leaves
Step V3: One more word is created on the V2 Stage to create three leaves
Step V4: One word is created on the V3 Stage to create three leaves
Step V5: One more word was created on the V4 Stage to create three leaves
Step R2: The state of the bean blooms
Step R4: Completion of pod creation
Step R5: Bean is produced in the pods
Step R6: Green beans in pods
Step R7: The state of the bean pods and beans turning yellow
Step R8: The state of the bean pods and beans turning yellow as the leaves fall
The composition according to claim 1, further comprising at least one selected from the group consisting of apigenin, coumestrol, caffeine, ginseng extract and green tea extract.
5. The method of claim 4,
When the composition contains an extract of green tea leaves and apigenin, the extract of green tea leaves and apigenin is contained in a mixing ratio of 1 to 10: 1 to 10 by weight,
Wherein the composition contains, in the case of containing the leaves of green bean leaf extract and coumestrol, the leaves of green bean leaf extract and cumestrol in a mixing ratio of 1 to 10: 1 to 10 by weight,
When the composition contains an extract of an extract of green tea leaves and caffeine, the extract of green tea leaves and caffeine in a mixing ratio of 1 to 10: 1 to 10 by weight,
When the composition contains the extract of the leaves of green tea leaves and ginseng extract, it contains the extract of green tea leaves and ginseng extract in a mixing ratio of 1 to 10: 1 to 10 by weight,
Wherein the composition contains, in the case of containing the extract of green tea leaves and green tea extract, the extract of green tea leaves and green tea extract in a mixing ratio of 1 to 10: 1 to 10 by weight.
The composition according to claim 1, wherein the colored leaves extract is contained in an amount of 1 to 60% by weight based on the total weight of the composition.
The composition according to claim 1, wherein the colored leaves extract promotes the activity of a Wnt / 3-catenin signaling system.
8. The composition according to any one of claims 1 to 7, wherein the composition is a pharmaceutical composition, a health functional food composition or a cosmetic composition.

Images