KR20140034964A - Composition for antiinflammation containing extract of soybean leaf - Google Patents

Abstract

The present invention relates to a composition for antiinflammation, antiallergy, atopic dermatitis treatment or improvement, moisturization, antibacterial, or hair follicle shrinkage. The composition of the present invention has antiinflammation effects which can treat or alleviate inflammation by suppressing or inhibiting the production of inflammation causing materials, and treat or alleviate allergies. In addition, the composition of the present invention can treat or improve the atopic dermatitis, and moisturize by promoting differentiation in keratinocytes so that the composition can be useful for treating or alleviating diseases related to xeroderma. Especially, the composition of the present invention has more enhanced effects of antiinflammation, antiallergy, atopic dermatitis treatment or improvement, or moisturization than bean extracts. the composition of the present invention merely has stimulation on organisms by including natural derived extracts obtained from natural materials.

Description

Anti-inflammatory composition comprising soybean leaf extract {COMPOSITION FOR ANTIINFLAMMATION CONTAINING EXTRACT OF SOYBEAN LEAF}

The present invention relates to an anti-inflammatory, anti-allergic, atopic treatment or improvement, moisturizing, antimicrobial or pore reduction composition comprising soybean leaf extract.

Human skin is the primary protective membrane of the human body. It functions to protect the internal organs from external environmental stimuli such as changes in temperature and humidity, ultraviolet rays, and pollutants. Depending on various internal and external factors, It undergoes change. In other words, internally, secretion of various hormones that regulate metabolism is reduced, the function of immune cells and the activity of cells are lowered, and the biosynthesis of immune proteins and biocompatible proteins necessary for the living body is reduced, and ozone layer destruction As the content of ultraviolet rays reaching the surface of the sunlight increases and environmental pollution is further intensified, free radicals and active noxious oxygen are increased, so that the thickness of the skin decreases, the wrinkles increase, the elasticity decreases Skin color is grayish, skin troubles occur frequently, and spots, freckles and black blots also increase.

In order to prevent changes in the skin condition caused by these internal and external factors and to maintain a healthy skin condition, the skin condition is improved by adding bioactive substances obtained from various known animals, plants, and microorganisms to cosmetics. Efforts have been made.

Accordingly, the present inventors have confirmed that the soybean leaf extract may provide an anti-inflammatory, anti-allergic effect as well as an atopic symptom improvement effect, and may provide a skin condition improvement effect such as a skin moisturizing function effect, thereby completing the present invention.

Republic of Korea Patent Publication No. 1998-077876

An object of the present invention is to provide a composition comprising a natural extract that can be used for anti-inflammatory, anti-allergic, atopic treatment or improvement or moisturizing.

In order to achieve the above object, the present invention provides an anti-inflammatory composition comprising a soybean leaf extract as an active ingredient.

The present invention also provides an anti-allergic composition comprising soybean leaf extract as an active ingredient, and provides a composition for treating or improving atopy comprising soybean leaf extract as an active ingredient and a moisturizing composition comprising soybean leaf extract as an active ingredient.

The composition of the present invention has an anti-inflammatory effect that can inhibit or inhibit the production of a causative agent of inflammation, thereby treating or alleviating inflammation, and is useful as it can treat or alleviate allergy. In addition, the composition of the present invention can treat or improve atopy, and has a moisturizing effect by promoting differentiation in keratinocytes, which is useful for the treatment or alleviation of symptoms associated with dry skin, and can relieve skin itching. have. In particular, the composition of the present invention shows a better effect than the soybean extract in anti-inflammatory, anti-allergic, atopic treatment or improvement or moisturizing ability. The compositions of the present invention also contain very little irritation to organisms, including extracts of natural origin obtained from natural products.

In the present specification, 'bean' is not limited in kind. In the composition which is one aspect of the present invention, specifically, the soybean of the present specification is selected from the group consisting of frosted, seomoktae, heuktae, chungtae, yellow, hedge bean, kidney bean, zebra kidney bean, red bean, soybean, bean sprout bean and soybean It may include any one or more, but is not limited thereto.

In the present specification, 'bean leaf' means the leaf portion of the bean tree.

In the present specification, the 'bean leaf extract' is, for example, in the extraction process, washed and dried soybean leaves or powdered soybean leaf powder and put it into water or an organic solvent to extract and deposit the residue and the filtrate through the filter cloth filtration and centrifugation Separation, and the separated filtrate can be concentrated under reduced pressure to obtain a soybean leaf extract. The organic solvent usable in the present invention may be selected from ethanol, methanol, butanol, ether, ethyl acetate, chloroform or a mixed solvent of these organic solvents and water, and considering the safety of the raw material, preferably water or 30 to 70% concentration. Ethanol is used. After obtaining the extract using a solvent in the above can be obtained by cooling, heating and filtration at room temperature in a conventional manner known in the art to obtain a liquid, or may further evaporate the solvent, spray drying or freeze drying, but The present invention is not limited thereto, and any method generally used in the art may be used without limitation.

The present invention relates to an anti-inflammatory composition comprising soybean leaf extract as an active ingredient in one aspect. The composition, which is an aspect of the present invention, can alleviate skin irritation and alleviate inflammation, and thus can be used for anti-inflammation. Specifically, the composition of the present invention may have an anti-allergic effect by inhibiting or inhibiting the expression of prostaglandin or interleukin, or by inhibiting or inhibiting the activity of prostaglandin or interleukin.

The present invention relates to an anti-allergic composition comprising soybean leaf extract as an active ingredient in one aspect. In one aspect of the present invention, the composition can treat or alleviate allergy by alleviating allergies or by inhibiting mechanisms in the body that cause allergies. Specifically, the composition of the present invention may have an anti-allergic effect by inhibiting or inhibiting the expression of prostaglandin or interleukin, or by inhibiting or inhibiting the activity of prostaglandin or interleukin.

The present invention relates to a composition for treating or improving atopy comprising soybean leaf extract as an active ingredient in one aspect. The composition, which is an aspect of the present invention, not only has anti-inflammatory and anti-allergic effects, but also can enhance skin barrier function and induce differentiation of dermal keratinocytes, Can be used for the treatment of visible atopy or for alleviation of atopic symptoms.

The present invention relates to a moisturizing composition comprising soybean leaf extract as an active ingredient in one aspect. The composition, which is an aspect of the present invention, may enhance skin barrier function and induce differentiation of keratinocytes of the skin to increase the moisture of the skin, thereby making the skin moist. Therefore, the composition, which is an aspect of the present invention, may be usefully used as a composition for preventing or improving skin dryness, atopic dermatitis, contact dermatitis, or psoriasis caused by incomplete epidermal differentiation.

The present invention relates to an antimicrobial composition comprising soybean leaf extract as an active ingredient in one aspect. The composition, which is one aspect of the present invention, specifically shows excellent antibacterial activity against acne bacteria. Therefore, the composition can be effectively used in the treatment and alleviation of acne.

The present invention relates to a composition for reducing pores comprising soybean leaf extract as an active ingredient in another aspect. Specifically, the composition of the present invention may increase the biosynthesis of collagen, thereby reducing the size of the pores.

The composition of the present invention means to alleviate the skin troubles or skin diseases caused by various reasons, to improve the condition, or to remove or inhibit various reasons causing skin troubles or skin diseases, It can improve the internal condition and is useful for improving skin condition.

The composition of the present invention can also be used as a composition for reducing pores, controlling sebum, and improving skin troubles. It reduces sebum secreted by the skin when applied to the skin, reduces active oxygen and promotes collagen synthesis, The reduction of the expression of inflammatory factors leads to an excellent effect of suppressing skin troubles.

In one aspect of the present invention, the composition may comprise 0.001 to 10% by weight of the bean leaf extract based on the total weight of the composition.

If the content of soybean leaf extract is less than 0.001% by weight based on the total weight of the composition, the skin troubles such as anti-inflammatory, anti-allergic and atopy are improved, and the skin moisturizing effect is insignificant. Because it does not. In view of the above, the composition of the present invention, 0.005 to 9.5% by weight, 0.01 to 9% by weight, 0.03 to 8.5% by weight, 0.05 to 8% by weight, 0.07 to 7.5% by weight, 0.09 to the total weight of the composition It may include 7% by weight, 0.1-6.5% by weight, 0.3-6% by weight, 0.5-5.5% by weight or 0.7-5% by weight of the bean leaf extract.

In the composition of one aspect of the invention, the soybean is Seoritae ( Glycin max MERR), Seomoktae (Seomoktae, Rhynchosia Nolubilis ), Black soybean, Glycine max (L.) Merr.), Cheongtae (blue bean, Glycime) max MERR), yellow bean, Glycime max MERR), field bean, Vicia faba ), Kidney bean ( Phaseolus) vulgaris , pinto bean, Phaseolus vulgaris L.), small red bean, Vigna angularis ), Soybeans (small black bean, Phaseolus angularis WF WIGHT.), Sprouting bean, Glycine max (L.) Merr.) and soybean ( Glycine) max ) and any one or more selected from the group consisting of.

In a composition which is one aspect of the present invention, the composition may inhibit or inhibit the production of prostaglandins. In one aspect of the present invention, the composition can inhibit or inhibit the production of prostaglandin itself, or inhibit or inhibit the activity of prostaglandins. The composition, which is an aspect of the present invention, may also inhibit or inhibit the activity of an up-stream enzyme or protein that produces prostaglandins.

In a composition which is one aspect of the present invention, the composition may inhibit or inhibit the production of interleukin. In one aspect of the invention, the composition may inhibit or inhibit the production of the interleukin itself or may inhibit or inhibit the activity of the interleukin. The composition, which is an aspect of the present invention, may also inhibit or inhibit the activity of an up-stream enzyme or protein that produces interleukin. Specifically, the interleukin may include interleukin-6 or interleukin-8, but is not limited thereto.

In a composition which is one aspect of the present invention, the bacterium includes acne bacteria.

In a composition which is one aspect of the present invention, the composition may promote biosynthesis of collagen. The composition may promote biosynthesis or increase activity of collagen itself, or increase or promote the activity of an enzyme or protein up-stream that produces collagen.

In one aspect of the invention, the composition comprises a cosmetic composition.

When formulating the external composition for skin according to the present invention in the form of cosmetics, soft cosmetics, astringent cosmetics, nourishing cosmetics, eye cream, nutrition cream, massage cream, cleansing cream, cleansing foam, cleansing water, powder, essence or pack, etc. It may be formulated in the form of, the formulation is not particularly limited. In addition, the composition according to the present invention can also be used as a lubricant, an organic solvent, a solubilizer, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, The active ingredient may be combined with any other ingredients commonly used in cosmetics, such as ionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, And may contain adjuvants commonly used in the same cosmetic or dermatological fields. Such adjuvants are introduced in amounts commonly used in the cosmetics or dermatological fields. In addition, the composition of the present invention may contain a skin absorption promoting substance to increase the skin improving effect.

The cosmetic composition according to the present invention may contain, in addition to the above-mentioned substances, other ingredients which can give a synergistic effect to the main effect, so long as they do not impair the main effect. The cosmetic composition according to the present invention may further comprise a moisturizing agent, an emollient agent, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an organic and inorganic pigment, a fragrance, a cold agent or a limiting agent. The blending amount of the above components can be easily selected by those skilled in the art within the range that does not impair the object and effect of the present invention, the blending amount may be 0.01 to 5% by weight, specifically 0.01 to 3% by weight based on the total weight of the composition. have.

In one aspect of the invention, the composition comprises a pharmaceutical composition.

When the composition according to the present invention is applied to medicines, it may be formulated into an oral or parenteral dosage form in the form of solid, semi-solid or liquid by adding an inorganic or organic carrier to the composition as an active ingredient.

Examples of the agent for oral administration include tablets, pills, granules, capsules, powders, fine granules, powders, emulsions, syrups and pellets. In addition, the preparations for parenteral administration include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, patches and the like. In order to formulate the active ingredient of the present invention, it can be easily formulated according to the conventional method. Surfactants, excipients, coloring agents, spices, preservatives, stabilizers, buffering agents, suspending agents and other adjuvants can be suitably used.

The pharmaceutical composition according to the present invention may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously and the like.

The effective ingredients of the pharmaceutical composition of the present invention will vary depending on the age, sex, weight, pathological condition and severity of the subject to be treated, administration route or judgment of the prescriber. Determination of the amount of application based on these factors is within the level of ordinary skill in the art and its daily dose is, for example, from 0.1 mg / kg / day to 100 mg / kg / day, more specifically from 5 mg / kg / day to 50 mg / / Day, but is not limited thereto.

In one aspect of the invention, the composition comprises a health food composition.

The composition according to the invention provides various types of food additives or functional foods comprising. Fermented milk, cheese, yogurt, juice, probiotic, tablets, granules, drinks, caramels, diet bars and the like containing the composition, and can be processed into conventional tea leaf form or tea bags and dietary supplements. And other various food additives.

In one embodiment, the composition may contain other ingredients and the like that can give a synergistic effect to the main effect within a range that does not impair the main effect of the present invention. For example, additives such as perfume, coloring agent, bactericide, antioxidant, preservative, moisturizing agent, thickening agent, inorganic salt, emulsifier and synthetic polymer substance may be further added for improvement of physical properties. In addition, supplementary ingredients such as water soluble vitamins, oil soluble vitamins, polymer peptides, polymer polysaccharides and seaweed extract may be further included. The above components may be mixed and selected without difficulty by those skilled in the art depending on the purpose of formulation or use, and the amount thereof may be selected within a range that does not impair the objects and effects of the present invention. For example, the addition amount of the above components may be in the range of 0.01 to 5% by weight, more specifically 0.01 to 3% by weight, based on the total weight of the composition.

The composition according to the present invention may be in various forms such as a solution, an emulsion, a viscous mixture, a tablet, a powder, etc., and may be administered by various methods such as simple drinking, injection administration, spraying or squeezing.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for illustrating the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

[ Manufacturing example  1] Preparation of Soybean Leaf Extract

1.5 kg of soybean leaves were pulverized with a blender, 7 L of an 80% ethanol aqueous solution was added thereto, and refluxed three times, followed by immersion at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation. The separated filtrate was concentrated under reduced pressure to obtain 216 g of a soybean leaf extract.

[ Comparative Example  1] Preparation of Soybean Extract

1.5 kg of beans were pulverized with a blender, and 7 L of an 80% ethanol aqueous solution was added thereto, followed by extraction under reflux three times, followed by immersion at 15 ° C. for 1 day. Thereafter, the residue and the filtrate were separated through filter cloth filtration and centrifugation. The separated filtrate was concentrated under reduced pressure to obtain 252 g of a soybean extract.

[ Test Example  1] improvement of inflammation

1. Inhibitory effect of prostaglandins

The anti - inflammatory effect was evaluated by the inhibitory effect of prostaglandin production. The effect was measured on macrophages using soybean leaf extract and soybean extract of Comparative Example 1. First, aspirin was added to the macrophage collected from mouse peritoneum to a final concentration of 500 uM to irreversibly inhibit the cyclooxygenase (COX) activity remaining in the cells. Then, the suspension was added to each well of a 96-well cell culture tube in an amount of 100 μl, followed by culturing in an incubator of 5% CO ₂ and 37 ° C for 2 hours to adhere macrophages to the surface of the container. Then, the adhered macrophages were washed three times with PBS and used for the inflammation improving effect test. After incubating for 12 hours by adding RPMI medium containing 1% (w / v) LPS to the cultured macrophages 5x10 ⁴ cells / ml, the production of prostaglandins was induced and 100 μl of the soybean leaf extract was treated to free prostaglandins. Quantification was performed using enzyme immunoassay (ELISA).

At this time, the production inhibitory activity of the prostaglandin of the soybean leaf extract was set to 100% the difference between the prostaglandin produced in the LPS treated group and the untreated group, and treated with LPS and the sample together to reduce the percentage of prostaglandin In comparison with, it was determined, and the result (the effect of inhibiting the production of prostaglandin) is shown in Table 1 below.

Test substance Prostaglandin inhibition (%) Blank 0% Control group (aspirin treatment group) 56.3% Bean extract 21.4% Soybean Leaf Extract 61.9%

As shown in Table 1, the experimental results showed that even when the soybean leaf extract treated as a control group treated with aspirin, the production inhibitory effect of prostaglandin is very high. In addition, it was found that the soybean leaf extract is more excellent in inhibiting the production of prostaglandins than the soybean extract. It was found that the soybean leaf extract of the present invention can provide an excellent inflammation improving effect. In addition, it was found that the soybean leaf extract can prevent and improve skin trouble by inhibiting the expression of prostaglandin, a skin inflammatory factor.

2. IL -8 generation inhibitory effect

The day before the experiment, normal human skin keratinocyte (NHEK, available from Lonza) was dispensed into a 96 well plate at 5 × 10 ⁴ cells / well and incubated at 37 ° C. in a 5% CO ₂ incubator And cultured for 24 hours. Twenty-four hours later, the cells were washed twice with PBS and replaced with serum free keratinocyte basement media (KBM). Soybean extract 50ppm and soybean leaf extract in each well was treated for 30 minutes by the concentration of Table 2, and then PGSA (10㎍ / ㎖) was treated in each well. Here, peptidoglycan from S. aureus (PGSA ) is a peptidoglycan extracted from Staphylococcus aureus, which is a major constituent of the cell wall of Gram-positive (+) bacteria, and the cell membrane components of bacteria are known to cause inflammation, Especially in staphylococcus, about 90% of atopic patients are reported to cause secondary infection by this bacterium. After culturing in a 5% CO ₂ incubator at 37 ° C for 24 hours, the culture was taken and ELISA for interleukin-8 (IL-8) was performed. The results are shown in Table 2 below. The ELISA used the experimental method of BD science.

Test substance IL-8 secretion (pg / ml) Untreated Control (Control) 932.19 PGSA (10 [mu] g / ml) 4831.20 Soybean Extract (50ppm) + PGSA (10µg / ml) 3987.37 Soybean Leaf Extract (5ppm) + PGSA (10µg / mL) 3832.38 Soybean Leaf Extract (25ppm) + PGSA (10µg / mL) 2412.41 Soybean Leaf Extract (50ppm) + PGSA (10µg / mL) 1477.25

In Table 2, it was confirmed that the soybean leaf extract significantly reduced and inhibited the secretion of IL-8 increased by PGSA and LPS. In addition, it was confirmed that the inhibitory effect of IL-8 secretion significantly higher than the soybean extract. Accordingly, it can be seen that the topical skin composition of the present invention can provide an excellent anti-inflammatory effect by significantly reducing the secretion of IL-8 increased by PGSA.

[ Test Example  2] Assess itching relief

The day before the experiment, keratinocytes (cell line name: HaCaT available from ATCC) were dispensed in a 96 well plate at 4x10 ⁴ cells / well and cultured in a 5% CO ₂ incubator at 37 ° C for 24 hours Respectively. After 24 hours, the 96-well plate was washed twice with HBSS (Hanks' Balanced Salt Solution) buffer, and the reaction buffer (2 μM Fluo-4-AM, 20% pluronic acid, 2.5 mM probenecid) gave. After reaction at 37 ° C., 5% CO ₂ incubator for 30 minutes, and at room temperature for 30 minutes, the cells were washed twice with HBSS buffer and treated with soybean leaf extract at the concentrations (%) shown in Table 3 below.

After reaction for 10 minutes to process the 2U / ml trypsin (Trypsin) or 5μM PAR-2 activation peptide (SLIGKV) and was measured in Ca ^{2 +} concentration in the cell 80 seconds. In Ca ^{2 +} concentration in the measurement cell presentation flex 3: was used (FlexStation 3 Molecular Device, USA) . After extracting soybean leaf extract, soybean extract and 2U / ml Trypsin or 5μM PAR-2 active peptide (SLIGKV) for 80 seconds, the difference between the minimum and maximum values was obtained. Table 3 shows the percent inhibition of calcium ions into the cell by comparing the difference between the minimum and maximum values of 2U / ml trypsin or 5 μM PAR-2 active peptide (SLIGKV).

Test substance
density Inhibition rate of intracellular influx of calcium ions (%) Trypsin (2 U / ml) PAR-2 active peptide (5 [mu] M) Bean Leaf Extract0.05% 25.2 18.4 Bean Leaf Extract 0.1% 31.4 25.7 Bean Leaf Extract 0.5% 45.7 48.3 Bean Extract 0.5% 25.9 16.4

As can be seen in Table 3, the influx of intracellular calcium ions by trypsin or PAR-2 active peptide (SLIGKV) was reduced by the treatment of soybean leaf extract, and the influx of intracellular calcium ions by increasing the concentration of soybean leaf extract. It was confirmed that this is significantly reduced. In addition, when compared with the soybean extract, it was confirmed that the soybean leaf extract has a significantly high inhibitory effect on calcium ion influx.

Therefore, the external preparation composition for skin containing soybean leaf extract of the present invention can provide an excellent antipruritic effect by effectively inhibiting PAR-2 activity causing itching.

[ Test Example  3] Antibacterial test for acne

Formulation Example 1 and Comparative Formulation Examples 1 to 3 were prepared according to the ingredients and the contents (% by weight) shown in Table 4 below. Specifically, Formulation Example 1 is a blend of soybean leaf extract, Comparative Formulation Example 1 does not contain any active ingredients for improving acne skin, Comparative Formulation Example 2 as a standard to be used as a reference for the antimicrobial activity, It contains erythromycin, a popular acne treatment. Comparative Formulation Example 3 is a formulation containing a soybean extract.

The preparation method of Formulation Example 1 and Comparative Formulation Examples 1-3 is as follows. The components of phase A in Table 4 were completely dissolved, and the components of phase B were completely dissolved in a separate dissolution bath, followed by mixing solubilization by adding phase B to phase A. The components of phase C were added thereto according to the blending ratios described in Table 4 to homogenize mixing and then filtered to prepare the compositions.

Composition statement (% by weight) Formulation Example  One Comparative Formulation Example  One Comparative Formulation Example  2 Comparative Formulation Example  3 A Purified water To  100 To  100 To  100 EDTA -2 Na 0.02 0.02 0.02 glycerin 5.0 5.0 5.0 B ethanol 2.0 2.0 2.0 PEG -60 Cured Castor Oil
( hydrogenated castor oil ) 0.4 0.4 0.4 Spices( perfume ) 0.04 0.04 0.04 C Soybean Leaf Extract 5.0 - - Erythromycin - - 5.0 Bean extract - - - 5.0

Antimicrobial activity was tested against propionibacterium acnes (ATCC 6919: Medium-BHI broth), an acne-causing strain, with each cosmetic composition prepared in the formulation of Formulation Example 1 and Comparative Formulation Examples 1-3.

The antibacterial test method for acne bacteria was as follows.

(1) Preparation of test strain

Propionibacterium acnes were cultured in an anaerobic culture inoculated on BHI broth.

(2) Dilution solution preparation

0.15 ml of the above test solution was added to 15 ml of BHI broth (pH 6.8) or LB broth (pH 4.5), and the mixture was used as a dilute solution.

(3) Preparation of sample

Cosmetic compositions prepared from Formulation Example 1 and Comparative Formulation Examples 1 to 3 were used as samples.

(4) Antimicrobial activity test

1) In a 96-well 96-well plate, place the sample in line 1 and add 200 μl of diluted solution.

2) Thoroughly mix the mixture of line 1, and then take 100 μl of the mixture, place it in the second row, mix well, and then repeat the double dilution by adding 100 μl to the third row.

3) After culturing the cells at 32 ° C for 24 hours and 48 hours, determine whether the bacteria are proliferating to the extent of suspension, and determine the minimum concentration without bacterial growth as the MIC (Minimum Inhibitory Concentration) value. If the mixed solution is opaque and it is difficult to determine the growth of bacteria, check through a microscope.

The antibacterial activity test results for acne bacteria are shown in Table 5 below. The MIC was expressed in terms of the concentration of the active ingredient contained in the formulation.

Item pH Propionibacterium Acnes Formulation Example 1 5.7 > 30 ppm Comparative Formulation Example 1 5.7 Maximum concentration (no antimicrobial activity) Comparative Formulation Example 2 5.7 > 90 ppm Comparative Formulation Example 3 5.7 > 200 ppm

The lower the ppm concentration in the MIC, the more effective the antimicrobial activity against acne bacteria. Formulation Example 1 was found to have a significantly lower ppm concentration than Comparative Formulation Example 2 using the known acne treatment erythromycin, it was confirmed that the composition containing the soybean leaf extract has a very good antibacterial activity. In addition, Formulation Example 1 containing the soybean leaf extract of the present invention was confirmed to have a significantly higher antimicrobial effect than Comparative Formulation Example 3 containing soybean extract .

[ Test Example  4] Improvement test of atopic symptoms in human body

In order to use the composition according to the composition of the present invention as a cosmetic, a composition containing a soybean leaf extract and a soybean extract was prepared with a cream having the composition specified in Table 6 below.

Ingredients (% by weight) Formulation Example 2 Comparative Formulation Example 4 Comparative Formulation Example 5 Purified water To 100 To 100 To 100 Soybean Leaf Extract 1.0 - - Bean extract - - 1.0 Vegetable hydrogenated oil 1.50 1.50 1.50 Stearic acid 0.60 0.60 0.60 Stearyl alcohol 2.00 2.00 2.00 Glycerol stearate 1.00 1.00 1.00 Polyglyceryl-10 < / RTI > pentastearate &
Behenyl alcohol and sodium stearoyl lactylate 1.00 1.00 1.00 Arachidyl behenyl alcohol and arachidyl glucoside 1.00 1.00 1.00 Cetylaryl Alcohol and Cetearyl Glucoside 2.00 2.00 2.00 PEG-100 Stearate & Glycerololate & Propylene Glycol 1.50 1.50 1.50 Caprylic / capric triglyceride 11.00 11.00 11.00 Cyclomedicone 6.00 6.00 6.00 Triethanolamine 0.1 0.1 0.1

In order to confirm the effect of improving atopic symptoms by the composition of the present invention, the cream prepared in Formulation Example 2 and Comparative Formulation Examples 4 to 5 was applied to the panels to evaluate the degree of atopic dermatitis improvement. For 20 panelists who had symptoms of dermatitis showing similar symptoms, divided into 10 groups of 3 people, and each composition of Table 6 above twice for 12 weeks at a temperature of 24 to 26 ° C and 75% humidity for 12 weeks. It was applied to the face. At the end of the test, subjects were asked to fill out a questionnaire and conducted a subjective efficacy evaluation. The questionnaire included symptoms of atopic dermatitis such as "pruritus", "dryness", "keratogenesis", "dandruff", "erythema", "swelling", "skin cracking", "tear and eczema", and "tanning". After dividing the above symptoms, the degree of improvement for each symptom was evaluated and averaged to evaluate the atopic symptoms. The results are shown in Table 7.

Application group (number of respondents - persons) Formulation Example 2 Comparative Formulation Example 4 Comparative Formulation Example 5 Significant improvement 3 Improving 6 2 4 No change One 8 6 worse

From Table 7, it can be seen that the composition containing the soybean leaf extract of the present invention further improves existing atopic dermatitis symptoms compared to Comparative Formulation Example 4. In addition, a significant atopic dermatitis improvement effect can be confirmed compared to the composition containing soy extract.

[ Test Example  5] pore reduction effect

1. Collagen biosynthesis promotes pore reduction effect

Collagen biosynthesis promoting effect of the soybean leaf extract according to the present invention was measured in comparison with TGF-β. First, fibroblasts were seeded at 10 ⁵ per hole in 24 wells and cultured until about 90% growth. After culturing with serum-free DMEM medium for 24 hours, the soybean leaf extract, soybean extract and TGF-β of the present invention dissolved in serum-free medium were treated with 10 mg / ml, respectively, and CO ₂ And cultured in an incubator for 24 hours. These supernatants were removed and procollagen increased or decreased using procollagen type I ELISA kit (procollagen type (I)). The results are shown in the following Table 8, and the combined performance of the collagen was set to 100 in the non-treatment group.

Test substance Collagen Total Performance (%) Untreated group 100 TGF-beta 183.5 Bean extract 142.6 Soybean Leaf Extract 178.2

As shown in Table 8, the soybean leaf extract of the present invention was confirmed to exhibit a collagen synthesis similar to the TGF-β positive control group, which was remarkable compared to the soybean extract. Therefore, it was confirmed that the soybean leaf extract according to the present invention can reduce the enlarged pores by increasing the amount of collagen production around the pores.

2. Pore reduction effect

In order to determine the pore reduction effect by the bean leaf extract of the present invention was evaluated as follows using the formulation example 2 and Comparative Formulation Examples 4-5 of Table 6. Thirty male and female subjects with large pore sizes were selected and divided into three groups of ten, and each of the nutritional creams of Formulation Example 2 and Comparative Formulation Examples 4 to 5 was applied to each face for four weeks. The evaluation of the effectiveness of the pore reduction was made by a visual evaluation of experts by taking pictures before and after the experiment. The results are shown in Table 9 below.

(Rating: 0-did not shrink at all; 5-very reduced)

Test substance Rating Rating Formulation Example 2 3.8 Comparative Formulation Example 4 One Comparative Formulation Example 5 1.5

From the results of Table 9, Comparative Formulation Examples 4 to 5 have little pore reduction effect, but in the case of Formulation Example 2, it was confirmed that the visible pore reduction effect was visible to the naked eye. Therefore, the soybean leaf extract according to the invention was found to be excellent in reducing the size of the pores.

Hereinafter, the formulation examples of the composition according to the present invention, but the pharmaceutical composition and cosmetic composition is applicable to a variety of formulations, which is intended to explain in detail only, not intended to limit the present invention.

[Formulation Example 1] Soft capsule

Soybean leaf extract 150 mg, palm oil 2 mg, palm hardened oil 8 mg, lead 4 mg and lecithin 6 mg were mixed, and 400 mg per capsule was filled according to a conventional method to prepare a soft capsule.

[Formulation Example 2] Tablets

150 mg of soybean leaf extract, 100 mg of glucose, 50 mg of red ginseng extract, 96 mg of starch and 4 mg of magnesium stearate were mixed and 40 mg of 30% ethanol was added to form granules, which were dried at 60 ° C. and purified using a tablet press. It was compressed into.

[Formulation Example 3] Granules

150 mg of soybean leaf extract, 100 mg of glucose, 50 mg of red ginseng extract, and 600 mg of starch were mixed, and 100 mg of 30% ethanol was added to form granules. The final weight of the contents was 1 g.

[Example 4] Drinks

150 mg of soybean leaf extract, 10 g of glucose, 50 mg of red ginseng extract, 2 g of citric acid and 187.8 g of purified water were mixed and filled into bottles. The final dose of the contents was 200 ml.

[Formulation Example 5] Preparation of health food

Soybean Leaf Extract ............................. 1000 mg

Vitamin mixture

Vitamin A Acetate ......... 70 μg

Vitamin E ............................................ 1.0 mg

Vitamin B1 ........................................... 0.13 mg

Vitamin B2 .......................................... 0.15 mg

Vitamin B6 ......................................... 0.5 mg

Vitamin B12 ......................... 0.2 μg

Vitamin C ............................................. 10 mg

Biotin ... 10 ㎍

Nicotinic acid amide .................................. 1.7 mg

Folic acid ................................................. ..... 50 μg

Calcium pantothenate .................................... 0.5 mg

Mineral mixture

Ferrous Sulfate ......................................... 1.75 mg

Zinc oxide ............................................ 0.82 mg

Magnesium carbonate ...................................... 25.3 mg

Potassium Phosphate ......................................... 15 mg

Secondary calcium phosphate ...................................... 55 mg

Potassium citrate ............................................ 90 mg

Calcium carbonate .............................................. 100 mg

Magnesium chloride ..................................... 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

Preparation Example 6 Preparation of Healthy Drinks

Soybean Leaf Extract ............................ 1000 mg

Citric acid ................................................. ... 1000 mg

oligosaccharide................................................. .... 100 g

Plum concentrate ................................................ ..... 2 g

Taurine ................................................. ........... 1 g

Purified water was added to the mixture to complete 900 ml

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was heated at 85 DEG C for about 1 hour with stirring, and the solution thus prepared was filtered to obtain a sterilized 2-liter container, which was sealed and sterilized, ≪ / RTI >

Although the composition ratio is mixed with a component suitable for a favorite beverage in a preferred embodiment, the compounding ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and use purpose. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

[Formulation Example 3] Softening Cosmetic (Skin Lotion)

Compounding ingredient Content (% by weight) Soybean Leaf Extract 0.1 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 Phage-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

Formulation Example 4 Nutrients (Milk Lotion)

Compounding ingredient Content (% by weight) Soybean Leaf Extract 0.1 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Wax 4.0 Polysorbate 60 1.5 Caprylic / capric triglyceride 5.0 Squalane 5.0 Sorbitacecequioleate 1.5 Liquid paraffin 0.5 Cetearyl alcohol 1.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water Balance

Formulation Example 5 Nutrition Cream

Compounding ingredient Content (% by weight) Soybean Leaf Extract 0.1 glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

Formulation Example 6 Massage Cream

Compounding ingredient Content (% by weight) Bean Leaf Extract 0.1 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 45.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Wax 4.0 Cetearyl glucoside 1.5 Sesquioleic acid sorbitan 0.9 Vaseline 3.0 paraffin 1.5 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 7] Pack

Compounding ingredient Content (% by weight) Soybean Leaf Extract 0.1 glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Allantoin 0.1 Nonyl phenyl ether 0.4 Polysorbate 60 1.2 Ethanol preservative 6.0 Good Preservative, coloring, fragrance Suitable amount Purified water Balance

Formulation Example 8 Ointment

Compounding ingredient Content (% by weight) Soybean Leaf Extract 0.1 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 1.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 Wax 4.0 Preservative, coloring, fragrance Suitable amount Purified water Balance

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will readily appreciate that many modifications are possible in the exemplary embodiments without materially departing from the novel teachings and advantages of this invention. something to do. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (15)

Anti-inflammatory composition comprising soybean leaf extract as an active ingredient. Anti-allergic composition comprising soybean leaf extract as an active ingredient. Atopic treatment or improvement composition comprising soybean leaf extract as an active ingredient. Moisturizing composition comprising soybean leaf extract as an active ingredient. Antimicrobial composition comprising soybean leaf extract as an active ingredient. Pore reduction composition comprising soybean leaf extract as an active ingredient. 7. The composition of claim 1, wherein the composition comprises from 0.001 to 10% by weight of soybean leaf extract, relative to the total weight of the composition. Article according to any one of the preceding claims, wherein the beans are seoritae (Seoritae, Glycin max MERR), seomoktae (Seomoktae, Rhynchosia Nolubilis ), Black soybean, Glycine max (L.) Merr.), Cheongtae (blue bean, Glycime) max MERR), yellow bean, Glycime max MERR), field bean, Vicia faba ), Kidney bean ( Phaseolus) vulgaris , pinto bean, Phaseolus vulgaris L.), small red bean, Vigna angularis ), Soybeans (small black bean, Phaseolus angularis WF WIGHT.), Sprouting bean, Glycine max (L.) Merr.) and soybean ( Glycine) max ) any one or more composition selected from the group consisting of. The composition of claim 1, wherein the composition inhibits or inhibits the production of prostaglandins. 7. The composition of claim 1, wherein the composition inhibits or inhibits the production of interleukin. 8. The composition of claim 5, wherein the bacterium is an acne bacterium. The composition of claim 6, wherein the composition promotes biosynthesis of collagen. The composition according to any one of claims 1 to 6, wherein the composition is a cosmetic composition. 7. The composition according to any one of claims 1 to 6, wherein the composition is a pharmaceutical composition. The composition of claim 1, wherein the composition is a health food composition.