KR20140033781A - Composition for improving condition of hair and scalp containing extract of soybean leaf - Google Patents

Abstract

The present invention relates to a composition comprising soybean leaf for preventing hair loss, promoting hair growth, or protecting scalp. The composition of the present invention can protect the scalp by alleviating itching by relieving skin irritation and by reducing dandruff. The composition of the present invention also can proliferate hair follicle cells and perform an opener role of potassium ion channels, thereby promoting the hair growth and preventing the hair loss.

Description

Technical Field [0001] The present invention relates to a composition for improving the condition of hair and scalp,

The present invention relates to a composition for preventing hair loss, for promoting wool, or for protecting scalp, which comprises an extract of soybean curd.

Hair loss is a problem that occurs through natural processes or is chemically facilitated through the use of certain therapeutic drugs designed to alleviate symptoms such as cancer. This hair loss involves a lack of hair regrowth leading to partial or complete baldness.

Recently, as more and more people with alopecia due to environmental pollution, stress, diet, fatigue, and inadequate eating habits, attention is paid to substances that can improve them. It was a challenge for the industry to find substances that inhibit hair loss, promote hair growth, and protect the scalp.

The inventors of the present invention confirmed that the composition containing the extract of soybean curd extract has the effect of preventing hair loss, promoting wool or protecting scalp, thus completing the present invention.

Fathke et al., "Wnt signaling induces epithelial differentiation during cutaneous wound healing ", BMC Cell Biology 2006, 7: 4

It is an object of the present invention to provide a composition comprising a natural extract which can be used for preventing hair loss or promoting wool or for protecting the scalp.

In order to achieve the above object, the present invention provides a composition for preventing hair loss or for promoting wool comprising an extract of soybean curd as an active ingredient.

The present invention also provides a composition for protecting scalp comprising an extract of soybean curd as an active ingredient.

The composition of the present invention can relieve skin irritation to relieve itching and reduce dandruff, thereby protecting the scalp. In addition, the composition of the present invention can proliferate oily cells of both hair follicles and can serve as an opener of potassium ion channels, thereby promoting wool and preventing hair loss. The composition of the present invention is also free from irritation to organisms including natural extracts obtained from natural products.

In this specification, the term 'bean' is not limited. In a composition according to one aspect of the present invention, the soybean of the present specification is selected from the group consisting of Seed Rice, Seomotei, Black Rice, Chungtae, Chrysanthemum, Fence, Soybean, Kidney Bean, But is not limited thereto.

In the present specification, 'canola' means the leaf part of the bean curd.

In the present invention, the 'canola leaf extract' is extracted in the extraction process by, for example, washing and drying the soap leaves or the kaolinized kaolin powder in water or an organic solvent, extracting it, and then filtering and separating the residue and the filtrate And the separated filtrate is concentrated under reduced pressure to obtain a soybean leaf extract. The organic solvent usable in the present invention may be selected from ethanol, methanol, butanol, ether, ethyl acetate, chloroform or a mixed solvent of these organic solvents and water. In view of the safety of raw materials, Of ethanol is used. After obtaining the extract by using the solvent, a liquid substance can be obtained by freezing, heating and filtering at room temperature by a conventional method known in the art. Alternatively, the solvent can be evaporated, spray dried or lyophilized But the present invention is not limited thereto, and any method generally used in the art can be used without limitation.

In one aspect, the present invention relates to a composition for preventing hair loss or promoting hair growth comprising an extract of soybean curd as an active ingredient.

As used herein, the term " hair " refers collectively to hair and hair, and may also be described as hair. The hair includes both human and animal hair.

As used herein, " promoting wool " means not only promoting the creation of new hair, but also allowing existing hair to grow coarser and healthier.

Specifically, in a composition according to one aspect of the present invention, the composition can alleviate skin irritation, reduce dandruff and alleviate skin itch, thereby preventing hair loss or promoting wool. The composition can also prevent hair loss or promote wool by either proliferating the hair cells or activating the potassium ion channels as openers of the potassium ion channels.

In another aspect, the present invention relates to a composition for protecting scalp comprising an extract of soybean curd as an active ingredient.

Specifically, in a composition that is an aspect of the present invention, the composition can alleviate skin irritation, particularly skin itch, and reduce dandruff. Therefore, the composition can be useful for the treatment of diseases such as atopic and psoriasis accompanied by itching of the skin.

In one aspect of the present invention, the composition may comprise from 0.001 to 10% by weight of the soybean leaf extract, based on the total weight of the composition.

If the content of the soybean leaf extract is less than 0.001% by weight based on the total weight of the composition, hair loss prevention effect or hair follicle stimulating effect or scalp protection effect is insignificant. If the content is more than 10% by weight, no significant increase in the effect is observed. In view of the above, the composition of the present invention is used in an amount of 0.005 to 9.5% by weight, 0.01 to 9% by weight, 0.03 to 8.5% by weight, 0.05 to 8% by weight, 0.07 to 7.5% by weight, 7 to 7 wt%, 0.1 to 6.5 wt%, 0.3 to 6 wt%, 0.5 to 5.5 wt%, or 0.7 to 5 wt% of the soybean leaf extract.

In one aspect of the invention, the beans are selected from the group consisting of Seoritae, Glycin max MERR, Seomoktae, Rhynchosia Nolubilis ), Black soybean, Glycine max (L.) Merr., blue bean, Glycime max MERR), yellow beans ( Glycime max MERR), field bean ( Vicia faba ), kidney bean (kidney bean, Phaseolus vulgaris , spotted beans (pinto bean, Phaseolus vulgaris L.), small red bean ( Vigna angularis ), small black beans ( Phaseolus angularis WF WIGHT.), sprouting beans ( Glycine max (L.) Merr.) and soybean (soybean, Glycine max ). < / RTI >

In one aspect of the present invention, the composition may alleviate scalp irritation.

In one aspect of the present invention, the composition may reduce dandruff.

In one aspect of the invention, the composition comprises a cosmetic composition.

Specifically, the cosmetic composition of the present invention may have conventional formulations and may be formulated into, for example, shampoos, rinses, hair tonics, pomades, hair treatments, hair lotions and the like. The hair cosmetic composition of each of these formulations may contain a cosmetic ingredient which is formulated in a conventional hair cosmetic composition according to the above-mentioned various formulations or for a final purpose, and the kind and amount of these ingredients can be easily selected by a person skilled in the art have.

When the composition according to the present invention is formulated in the form of a cosmetic, it may be in the form of softening agent, convergent lotion, nutritional lotion, eye cream, nutritional cream, massage cream, cleansing cream, cleansing foam, cleansing water, powder, essence or pack , And the formulation is not particularly limited. In addition, the composition according to the present invention can also be used as a lubricant, an organic solvent, a solubilizer, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, The active ingredient may be combined with any other ingredients commonly used in cosmetics, such as ionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, And may contain adjuvants commonly used in the same cosmetic or dermatological fields. Such adjuvants are introduced in amounts commonly used in the cosmetics or dermatological fields. In addition, the composition of the present invention may contain a skin absorption promoting substance to increase the skin improving effect.

The cosmetic composition according to the present invention may contain, in addition to the above-mentioned substances, other ingredients which can give a synergistic effect to the main effect, so long as they do not impair the main effect. The cosmetic composition according to the present invention may further comprise a moisturizing agent, an emollient agent, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an organic and inorganic pigment, a fragrance, a cold agent or a limiting agent. The compounding amount of the above components can be easily selected by those skilled in the art within a range not to impair the objects and effects of the present invention. The amount thereof may be 0.01 to 5% by weight, specifically 0.01 to 3% by weight, have.

In one aspect of the invention, the composition comprises a pharmaceutical composition.

When the composition according to the present invention is applied to medicines, it may be formulated into an oral or parenteral dosage form in the form of solid, semi-solid or liquid by adding an inorganic or organic carrier to the composition as an active ingredient.

Examples of the agent for oral administration include tablets, pills, granules, capsules, powders, fine granules, powders, emulsions, syrups and pellets. In addition, the parenteral administration may include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, pills, and the like. In order to formulate the active ingredient of the present invention, it can be easily formulated according to the conventional method. Surfactants, excipients, coloring agents, spices, preservatives, stabilizers, buffering agents, suspending agents and other adjuvants can be suitably used.

The pharmaceutical composition according to the present invention may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously and the like.

The effective ingredients of the pharmaceutical composition of the present invention will vary depending on the age, sex, weight, pathological condition and severity of the subject to be treated, administration route or judgment of the prescriber. Determination of the amount of application based on these factors is within the level of ordinary skill in the art and its daily dose is, for example, from 0.1 mg / kg / day to 100 mg / kg / day, more specifically from 5 mg / kg / day to 50 mg / / Day, but is not limited thereto.

In one aspect of the invention, the composition comprises a health food composition.

The composition according to the present invention provides various types of food additives or functional foods containing them. The composition may be formulated into fermented milk, cheese, yogurt, juice, probiotics, tablets, granules, drinks, caramels, diet bars, and the like, and may be processed into ordinary tea leaves, tea bags, And can be used in the form of various other food additives.

In one embodiment, the composition may contain other ingredients or the like that can give synergistic effects to the main effect within a range that does not impair the intended main effect of the present invention. For example, additives such as perfume, coloring agent, bactericide, antioxidant, preservative, moisturizing agent, thickening agent, inorganic salt, emulsifier and synthetic polymer substance may be further added for improvement of physical properties. In addition, it may further contain auxiliary components such as water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides and seaweed extract. The above components may be mixed and selected without difficulty by those skilled in the art depending on the purpose of formulation or use, and the amount thereof may be selected within a range that does not impair the objects and effects of the present invention. For example, the addition amount of the above components may be in the range of 0.01 to 5% by weight, more specifically 0.01 to 3% by weight, based on the total weight of the composition.

The composition according to the present invention may be in various forms such as a solution, an emulsion, a viscous mixture, a tablet, a powder, etc., and may be administered by various methods such as simple drinking, injection administration, spraying or squeezing.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for illustrating the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

[ Manufacturing example  1] Preparation of soybean leaf extract

1.5 kg of bean leaves were pulverized with a blender, and 7 liters of an 80% aqueous ethanol solution was added thereto. The mixture was refluxed three times and then immersed at 15 ° C for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 216 g of an extract of the bean leaf.

[ Comparative Example  1] Production of soybean extract

1.5 kg of soybean was pulverized with a mixer, and 7 liters of 80% aqueous ethanol solution was added thereto, refluxed three times, and then immersed at 15 ° C for 1 day. Thereafter, the filtrate and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain 252 g of a soybean extract

[ Example  1 to 4 and Comparative Example  1, 2] Manufacture of shampoo

Shampoos were prepared with the ingredients and composition ratios shown in Table 1 below.

Specifically, a surfactant and ethylene glycol distearate were added to purified water, heated to 80 DEG C to dissolve uniformly, and gradually cooled to 40 DEG C with stirring. To the mixture, , A viscosity adjusting agent, a perfume, and a hair conditioning agent were mixed and stirred, followed by cooling to room temperature.

ingredient Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 Ammonium lauryl sulfate 10 10 10 10 10 10 Polyoxyethylene lauryl ammonium sulfate 5 5 5 5 5 5 Cocoamidopropyl betaine 2 2 2 2 2 2 Ethylene glycol distearate 1.5 1.5 1.5 1.5 1.5 1.5 Cocoyl monoethanolamide 0.8 0.8 0.8 0.8 0.8 0.8 Soybean extract 1.0 2.0 3.0 5.0 - - Soybean extract - - - - - 5.0 Polyquaternium-10 0.2 0.2 0.2 0.2 0.2 0.2 Blue No. 1 0.0002 0.0002 0.0002 0.0002 0.0002 0.0002 Yellow No. 4 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 Methylparaben 0.1 0.1 0.1 0.1 0.1 0.1 Spices 0.8 0.8 0.8 0.8 0.8 0.8 Citric acid 0.1 0.1 0.1 0.1 0.1 0.1 Dimethicone 1.0 1.0 1.0 1.0 1.0 1.0 water to 100 to 100 to 100 to 100 to 100 to 100

[ Test Example  1] Stimulation relaxation efficacy test

To evaluate the effectiveness of stimulation by shampoo stimulation, the MEST method using a mouse was applied and the test method was as follows.

Six - week - old BALB / c mice were purchased and the test system was constructed with 10 - day quarantine and adaptation period. The hair was removed from the abdomen of the rats and 2.5% para-phenylenediamine (PPD) was applied for 3-5 days. After a week's resting period, the shampoo was applied to the rat's ear and wiped off after 30 minutes. After a resting period of one week, the compositions of Examples 1 to 4 and Comparative Examples 1 and 2 were applied twice at intervals of one hour. After shampooing again, the compositions of Examples 1 to 4 and Comparative Examples 1 to 2 were applied twice for 24 hours. After 24 hours, the weight of the ear was measured, and the weight of the ear that was pathologically organized by H & E staining was measured. The measurement results are shown in Table 2 below.

Ear Weight (mg) Ear thickness (μm) Example 1 9.71 + - 0.58 275.43 + - 24.51 Example 2 9.59 ± 0.65 251.11 ± 22.30 Example 3 9.21 ± 0.78 245.40 +/- 24.30 Example 4 8.17 ± 1.67 237.51 ± 25.82 Comparative Example 1 14.91 + 1.61 454.22 + - 11.59 Comparative Example 2 11.31 ± 1.21 314.22 + 16.59

As can be seen from the above Table 2, it was confirmed that the ear weight and the ear thickness decreased significantly by pretreating with the compositions of Examples 1 to 4 according to the present invention. In addition, the compositions of Examples 1 to 4 of the present invention showed better effects than those of Comparative Example 2 containing soybean extract. Thus, it can be seen that the composition for improving hair and scalp according to the present invention has an effect of relieving inflammation.

[ Test Example  2] Dandruff  evaluation

The anti-dandruff strengths of Examples 1 to 4 and Comparative Examples 1 and 2 were measured and compared. At this time, as test strains, Pseudosodium formate ( Pityrosporum Ovalae (ATCC 12078)) was used. The skin disc diffusion method was used to measure the antibacterial activity. The skin disc diffusion method (SDDM) is the method closest to the consumer's habits and is a method of measuring the antimicrobial activity against guinea pig skin similar to human skin. Specifically, guinea pig skin was peeled off, treated with 70% ethanol, uniformly spread, and skin disks cut to a certain size were used. The sterilized skin discs were immersed in the shampoo dilution solution for 3 minutes and then rinsed with flowing water. Then, the skin discs were placed on a solid medium inoculated with the test bacteria, and then the test bacteria were cultured. After the culture, the size of the clear zone in which the growth of the bacteria was inhibited was measured, and the relative antibacterial activity was measured. Experimental results are shown in Table 3 below as the average value of the results of three measurements.

division Restraining Size (mm) Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 Strain Reduction Size 3.5 3.7 4.6 5.2 0 2.8

In Table 3, Comparative Example 1 containing no soybean leaf extract had no effect of reducing dandruff, but Examples 1 to 4 containing soybean leaf extract effectively reduced dandruff. Compared with Comparative Example 2 containing soybean extracts, Examples 1 to 4 of the present invention were found to be more effective in reducing dandruff.

[ Test Example  3] Dandruff reduction effect test

Twenty-four men aged 19 to 35 years with a relatively large number of dandruff were selected, and the shampoos of Examples 1 to 4 and Comparative Examples 1 and 2 were used in 5 groups of 10 persons for 1 month in the following manner, Were measured.

Before the start of the test, the dandruff was sampled with ordinary shampoo, and the dandruff accumulated for 2 days after shaking was sampled. The dandruff weight was sampled once every two days with the shampoo of each of Example 4 and Comparative Examples 1 and 2, The weights of accumulated dandruff for days were compared and evaluated. The accumulated dandruff was collected directly from the scalp using a vacuum suction device, and the dandruff reduction rate was calculated according to the following equation (1). The results are shown in Table 4 below.

[Equation 1]

Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 Dandruff reduction rate (%) 71.83 68.25 75.19 86.52 1.40 61.83 71.20 60.69 82.22 86.52 -0.40 61.20 54.50 73.08 64.69 93.03 -1.00 44.50 54.19 63.73 86.63 80.01 1.60 44.19 62.17 55.65 83.69 73.81 2.30 52.17 56.08 74.76 78.55 84.84 0.80 46.08 52.51 63.21 69.20 79.06 6.40 42.51 71.09 66.15 66.47 80.07 3.70 61.09 55.87 67.62 78.86 89.88 3.40 45.87 63.01 59.74 82.43 90.51 4.70 53.01 medium 61.25 65.29 76.79 84.43 2.29 51.25 SD 7.74 5.93 7.65 6.05 2.30 7.74

As can be seen from Table 4, Examples 1 to 4 containing soybean leaf extract exhibit excellent dandruff prevention effect. It was also found that the extracts of Examples 1 to 4 of the present invention exhibit more excellent dandruff prevention effect than Comparative Example 2 containing soybean extract.

[ Test Example  4] Scalp itching prevention effect test

Seventy two men and women aged 25 to 45 years who were relatively scarred with scalp itching were selected and divided into 6 groups of 12 persons. Each shampoo of Example 4 and Comparative Examples 1 and 2 was used once every three days for 2 weeks, The itching prevention effect was evaluated through the following evaluation criteria, and the results are shown in Table 5 below.

[Evaluation standard]

Very good - 5 points

Excellent - 4 points

Usually - 3 points

Poor - 2 points

Very bad - 1 point

division Example 4 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 The itching effect of scalp 3.2 3.4 4.1 4.6 2.2 3.2

As can be seen from Table 5, it can be seen that Examples 1 to 4 containing the soybean leaf extract exhibit excellent effects in preventing scalp itching. In addition, Examples 1 to 4 containing the soybean leaf extract exhibited more excellent scalp itching inhibitory effect than Comparative Example 2 containing the soybean extract.

[ Test Example  5] hair follicles Dairy cattle  Cell proliferation effect

The keratin proteins that make up the hair are produced in hair follicle keratinocyte, which keratinocytes differentiate from the dermal papilla cells. In order to evaluate the activity of the dermal papilla cells of the present composition, DP6 (rat immortalized dermal papilla cell) cell line was used in the present invention (Wendy Filsell, Journal of Cell Science 107, 1761-1772 (1994)). This dermal papilla cell line was obtained by microdissection in the hair roots of male PVG rat beard and was cultured in DMEM (Dulbecco's modified Eagle's medium, Gibco BRL, Gaithersburg, MD, USA) containing 10% FBS (Fetal bovine serum) USA) for 24 hours in an incubator maintained at 37 ° C in 5% CO 2. DP6 was added to a 96-well plate and cultured in a 37 ° C incubator for 24 hours. Then, the extracts were treated at a concentration of 10 ppm, 50 ppm and 100 ppm and a soybean extract concentration of 100 ppm, respectively. After 24 hours of drug treatment, cell proliferation was measured using WST-1 kit (Roche). The results are shown in Table 6 below.

division Cell proliferation (%) Untreated Control (Control) 100 Soybean extract (10ppm) 117 Soybean extract (50ppm) 135 Soybean extract (100ppm) 156 Soybean extract (100ppm) 117

As can be seen from Table 6 above, the proliferation of dermal papilla cells was increased in the case of treatment with the soybean leaf extract, and it was confirmed that this increased significantly in a concentration-dependent manner. In addition, when the soybean extract was treated with soybean extract, the growth of the dermal papilla cells was more excellent than that of soybean extract.

[ Test Example  6] Assessment of Potassium Ion Channel Activity Increase Effect

Minoxidil, a therapeutic agent for depilation, is known as a potential mitochondrial potassium ion channel opener (KATP channel opener) and is a representative drug used for the treatment of androgenetic alopecia. To evaluate the mechanism of minoxidil, cytotoxicity was inhibited by treatment with tolbutamide (SIGMA AlDRICH, T0891) blocking the KATP channel in fibroblasts constituting the dermis of the scalp, and the cell proliferation was restored by opening the potassium ion channel again Was used.

To evaluate the function of the present composition as a KATP channel opener, the fibroblast cell line NIH3T3 (mouse embryonic fibroblast cell line) cell line was used in the present invention. This cell line is a cell line obtained by natural immortalization of fibroblasts isolated from NIH Swiss mouse embryo by the 3T3 protocol. The cell line was cultured in DMEM (Gibco BRL, Gaithersburg, Md., USA) containing 10% FBS for 24 hours in an incubator maintained at 37 ° C in 5% CO2. NIH3T3 was cultured in a 37 ° C incubator for 24 hours and then treated with 2.5 mM tolbutamide. After 10 minutes, 10 μM of minoxidil, a positive control, and soybean leaf extract were added at concentrations of 10 ppm, 50 ppm and 100 ppm, 100 ppm of soybean extract , And cell proliferative activity was measured using a WST-1 kit (Roche) 48 hours after the drug treatment. The results are shown in Table 7 below.

division Cell proliferation (%) Untreated Control (Control) 100 Minoxidil 125 Soybean extract (10ppm) 103 Soybean extract (50ppm) 115 Soybean extract (100ppm) 126 Soybean extract (100ppm) 110

As can be seen from the above Table 7, it can be understood that when the extract of canola leaves is treated, the proliferation of fibroblasts is restored and the cell proliferation ability is increased depending on the concentration of the treated soybean leaf extract. When the soybean leaf extract is treated at 100 ppm, And the proliferation of the fibroblasts is restored to the level. In addition, when the soybean extract was treated with soybean extract, the proliferation of fibroblast cells was more excellent than that of soybean extract.

[ Example  5 and Comparative Example  3, 4]

The composition of the cream formulation was prepared in a conventional manner according to the composition of Table 8 below (unit: wt%).

Compounding ingredient Example 5 Comparative Example 3 Comparative Example 4 Labrafac 23 23 23 Tween 80 10.5 10.5 10.5 Glycerol monostearate 4.0 4.0 4.0 Stearic acid 7.0 7.0 7.0 Cetyl alcohol 2.0 2.0 2.0 Propylene glycol 9.0 9.0 9.0 Glycerol monooleate 3.5 3.5 3.5 Soybean extract One - - Soybean extract - - One water 40 41 40

[ Test Example  7] Wool effect of soybean leaf extract

The hair of the back of the ICR mouse was depilated by a depilator, and hair was completely removed by using a depilatory cream (bit cream). The other two groups except the control group (normal group) were applied to the skin once a day with 200 쨉 l of 1% DNCB to induce skin inflammation for 3 days. In order to minimize the hair growth by setting stress conditions in the skin by inducing skin irritation by this method and to evaluate the hair regrowing effect of the composition of the present invention, in Example 5 and Comparative Examples 3 to 5, 4, and the length of the hair was measured according to the number of days of treatment and compared with the control group. The results are shown in Table 9 below.

Days to be treated (days) Fur Length (cm) Example 5 Treatment Comparative Example 3 Treatment Comparative Example 4 Treatment 0 0.07 0.07 0.07 6 0.21 0.07 0.10 9 0.52 0.07 0.13 12 0.81 0.07 0.15

As shown in Table 9, the hair length of the group treated with Example 5 showed a statistically significant difference (p < 0.01) as compared with the group treated with Comparative Example 3. In addition, the hair length of the group treated with Example 5 increased to 0.81 cm after 12 days from the treatment, depending on the treatment days. Therefore, it was confirmed that the group treated with Example 5 had longer fur than the group treated with Comparative Example 3 which did not contain the soybean leaf extract. Furthermore, it was confirmed that the hair length was remarkably increased in the group treated with Example 5 of the present invention containing the soybean leaf extract, as compared with Comparative Example 4 containing the soybean extract.

It was concluded that the extracts of soybean curd extract promoted hair regeneration even under conditions of restoration of hair regrowth under skin stress. Thus, it can be seen that the extract of soybean curd extract has a regenerating function against hair loss under stress.

[ Test Example  8] Prevention and treatment of white algae extract of in-vivo canola extract using white algae-stimulated albumin mice Black hair  Evaluation of induction efficacy

Vitiligo mice (C57bl / 6-Mitfmi-vit) were purchased from The Jackson Lab, USA. The anti-whiplash effect test method using the mouse in which the white moth is promoted is as follows. Dorsal hairs of 12-week-old mice were removed by depilation. However, the width of the hair removal area was adjusted to be the same for each individual. From the day after the hair was pulled out, twice a day, the anti-whiplash material was applied on the hairless area. A mixture of EtOH: 1,3-BG: DW = 3: 2: 5 (volume ratio) was used as the vehicle of the anti-whiplash material. The carrier was used as a negative control, and a solution containing 50 mM of IBMX As a control group, 2.5% of soybean leaf extract and 2.5% of soybean extract were used as a test group.

After 3 weeks, the difference in antibacterial efficacy between each substance was visually identified. The newly emerged hairs were collected and the amount of melanin in the hair was measured. The amount of melanin in hair was measured using the protein hydrolyzing enzyme Esperase (Novozyme). Reaction buffer was prepared by dissolving Esperase in a buffer (50 mM Tris-HCl, 5 mM DTT, pH 9.3) to a concentration of 1 NPU / ml. Five milligrams of mouse hair was added to 1 ml of reaction buffer and allowed to react for 13 hours at 37 ° C with shaking at 1,000 rpm. Then, the hair and the reaction solution were separated by an instant centrifugation. The reaction solution thus obtained was placed in a 96-well plate and the absorbance at 405 nm was measured to determine the amount of melanin in the reaction solution. Table 11 shows the results of visual inspection and measurement of melanin in hair by treatment of negative control, positive control, and test group material, respectively, with a white algae mouse model in which white spot generation was promoted.

Negative control group IMBX Soybean extract Soybean extract The% 100 106.7 108.8 101.5

According to Table 11, it was found that the extract of canola leaves inhibits white moths in the mouse body promoted to white moth and increases the amount of melanin in the hair to promote the black moth induction. In addition, the extracts of soybean curd extracts were significantly superior to those of soybean extracts.

Hereinafter, a formulation example of the composition according to the present invention will be described. However, the pharmaceutical composition and the cosmetic composition can be applied to various formulations, which are not intended to limit the present invention but merely to illustrate the present invention.

[Formulation Example 1] Soft capsule

Soft bean capsule was prepared by mixing 150 mg of soybean leaf extract, 2 mg of palm oil, 8 mg of palm kernel oil, 4 mg of yellow radish and 4 mg of lecithin and 400 mg per capsule according to a conventional method.

[Formulation Example 2] Tablets

The granules were prepared by mixing 150 mg of soybean leaf extract, 100 mg of glucose, 50 mg of red ginseng, 96 mg of starch and 4 mg of magnesium stearate and 40 mg of ethanol was added thereto, followed by drying at 60 ° C., Respectively.

[Formulation Example 3] Granules

The granules were formed by mixing 150 mg of soybean leaf extract, 100 mg of glucose, 50 mg of red ginseng extract and 600 mg of starch, and 100 mg of 30% ethanol was added thereto, followed by drying at 60 ° C to form granules. The final weight of the contents was 1 g.

[Formulation Example 4] Drinking agent

150 mg of soybean leaf extract, 10 g of glucose, 50 mg of red ginseng extract, 2 g of citric acid and 187.8 g of purified water were mixed and filled in a bottle. The final volume of the contents was adjusted to 200 ml.

[Formulation Example 5] Preparation of health food

Soybean leaf extract .............................. 1000 mg

Vitamin mixture

Vitamin A Acetate ....................... 70 ㎍

Vitamin E ............................................ 1.0 mg

Vitamin B1 ........................................... 0.13 mg

Vitamin B2 .......................................... 0.15 mg

Vitamin B6 ........................................... 0.5 mg

Vitamin B12 .............................. 0.2 g

Vitamin C ............................................. 10 mg

Biotin ................................................. 10 [mu] g

Nicotinic acid amide .................................. 1.7 mg

Folic acid ................................................. ..... 50 μg

Calcium pantothenate .................................... 0.5 mg

Mineral mixture

Ferrous sulfate .......................................... 1.75 mg

Zinc oxide ............................................ 0.82 mg

Magnesium carbonate ...................................... 25.3 mg

Potassium Phosphate ......................................... 15 mg

Secondary calcium phosphate ...................................... 55 mg

Potassium citrate ............................................ 90 mg

Calcium carbonate .............................................. 100 mg

Magnesium chloride ..................................... 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixture is comparatively mixed with a composition suitable for health food as a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional method for producing healthy foods , Granules can be prepared and used in the manufacture of health food compositions according to conventional methods.

[Formulation Example 6] Preparation of health drink

Canned Bean Extract ............................ 1000 mg

Citric acid ................................................. ... 1000 mg

oligosaccharide................................................. .... 100 g

Plum concentrate ................................................ ..... 2 g

Taurine ................................................. ........... 1 g

Purified water was added to the mixture to complete 900 ml

The above components were mixed according to a conventional health drink manufacturing method, and the mixture was heated for about 1 hour at 85 DEG C with stirring, and the solution thus prepared was filtered to obtain a sterilized 2-liter container, which was sealed and sterilized, &Lt; / RTI &gt;

Although the composition ratio is relatively mixed with a component suitable for a favorite drink, it is also possible to arbitrarily modify the blending ratio according to the regional or national preference such as the demand level, the demanding country, the use purpose, and the like. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

[Formulation Example 1] Softening lotion (skin lotion)

Compounding ingredient Content (% by weight) Soybean extract 0.1 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 Phage-12 nonylphenyl ether 0.2 Polysorbate 80 0.4 ethanol 10.0 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 2] Nutritional lotion (Milk lotion)

Compounding ingredient Content (% by weight) Soybean extract 0.1 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Wax 4.0 Polysorbate 60 1.5 Caprylic / capric triglyceride 5.0 Squalane 5.0 Sorbitacecequioleate 1.5 Liquid paraffin 0.5 Cetearyl alcohol 1.0 Triethanolamine 0.2 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 3] Nourishing cream

Compounding ingredient Content (% by weight) Soybean extract 0.1 glycerin 3.0 Butylene glycol 3.0 Liquid paraffin 7.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 5.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Polysorbate 60 1.2 Triethanolamine 0.1 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 4] Massage cream

Compounding ingredient Content (% by weight) Soybean extract 0.1 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 45.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Wax 4.0 Cetearyl glucoside 1.5 Sesquioleic acid sorbitan 0.9 Vaseline 3.0 paraffin 1.5 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 5] Pack

Compounding ingredient Content (% by weight) Soybean extract 0.1 glycerin 4.0 Polyvinyl alcohol 15.0 Hyaluronic acid extract 5.0 Beta Glucan 7.0 Allantoin 0.1 Nonyl phenyl ether 0.4 Polysorbate 60 1.2 Ethanol preservative 6.0 Good Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 6] ointment

Compounding ingredient Content (% by weight) Soybean extract 0.1 glycerin 8.0 Butylene glycol 4.0 Liquid paraffin 15.0 Beta Glucan 7.0 Carbomer 0.1 Caprylic / capric triglyceride 3.0 Squalane 1.0 Cetearyl glucoside 1.5 Sorbitan stearate 0.4 Cetearyl alcohol 1.0 Wax 4.0 Preservative, coloring, fragrance Suitable amount Purified water Balance

[Formulation Example 6] Shampoo

Compounding ingredient Content (% by weight) Ammonium lauryl sulfate 10 Polyoxyethylene lauryl ammonium sulfate 5 Cocoamidopropyl betaine 2 Ethylene glycol distearate 1.5 Cocoyl monoethanolamide 0.8 Soybean extract 5.0 Polyquaternium-10 0.2 Blue No. 1 0.0002 Yellow No. 4 0.0001 Methylparaben 0.1 Spices 0.8 Citric acid 0.1 Dimethicone 1.0 water to 100

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will readily appreciate that many modifications are possible in the exemplary embodiments without materially departing from the novel teachings and advantages of this invention. something to do. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (9)

A composition for preventing hair loss or promoting wool comprising an extract of soybean curd as an active ingredient. A composition for protecting scalp comprising an extract of soybean curd as an active ingredient. 3. The composition of claim 1 or 2, wherein the composition comprises from 0.001 to 10% by weight of the soybean leaf extract, based on the total weight of the composition. 3. The method according to claim 1 or 2, wherein the soybean is selected from the group consisting of Seoritae, Glycin max MERR), Seomoktae ( Rhynchosia Nolubilis , Black soybean, Glycine max (L.) Merr., Blue bean, Glycime max MERR), yellow beans ( Glycime max MERR), field beans ( Vicia faba , kidney bean, Phaseolus vulgaris , pinto bean, Phaseolus vulgaris L.), small red bean ( Vigna angularis ), small black beans ( Phaseolus angularis WF WIGHT.), sprouting beans ( Glycine max (L.) Merr.) and soybean (soybean, Glycine max ). &lt; / RTI &gt; 3. The composition of claim 1 or 2, wherein the composition alleviates scalp irritation. 3. The composition of claim 1 or 2, wherein the composition reduces dandruff. 3. The composition of claim 1 or 2, wherein the composition is a cosmetic composition. 3. The composition of claim 1 or 2, wherein the composition is a pharmaceutical composition. 3. The composition of claim 1 or 2, wherein the composition is a health food composition.