KR20150036917A - Composition for improving condition of hair and scalp containing polysaccharides extracted from processed Panax ginseng and Green tea - Google Patents

Composition for improving condition of hair and scalp containing polysaccharides extracted from processed Panax ginseng and Green tea Download PDF

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KR20150036917A
KR20150036917A KR20130115936A KR20130115936A KR20150036917A KR 20150036917 A KR20150036917 A KR 20150036917A KR 20130115936 A KR20130115936 A KR 20130115936A KR 20130115936 A KR20130115936 A KR 20130115936A KR 20150036917 A KR20150036917 A KR 20150036917A
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ginseng
green tea
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composition
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이옥찬
김동현
황경환
염명훈
조준철
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(주)아모레퍼시픽
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9789Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/25Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
    • A61K36/258Panax (ginseng)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/82Theaceae (Tea family), e.g. camellia
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q5/00Preparations for care of the hair
    • A61Q5/002Preparations for repairing the hair, e.g. hair cure
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q5/00Preparations for care of the hair
    • A61Q5/006Antidandruff preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10Preparation or pretreatment of starting material

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Abstract

The present invention relates to a composition including a ginseng polysaccharide and a green tea polysaccharide for improvement of hair or scalp conditions. More specifically, the present invention relates to a composition which uses a mixture obtained by mixing polysaccharides extracted from an extract of preprocessed ginseng and green tea. Accordingly, the composition prevents grey hair and hair loss, alleviates scalp irritation and inhibits dandruff, scalp inflammation, etc, thereby improving general hair conditions and having an excellent cleaning and calming effect on the scalp. The composition includes the ginseng polysaccharide and the green tea polysaccharide separately in a content of 0.001-10 wt% with respect to the total weight of the composition. The ginseng polysaccharide and the green tea polysaccharide are mixed in the weight ratio of 0.1-10:1.

Description

포제 처리한 인삼 및 녹차에서 추출한 다당체를 함유하는 모발 또는 두피 상태 개선용 조성물{Composition for improving condition of hair and scalp containing polysaccharides extracted from processed Panax ginseng and Green tea}TECHNICAL FIELD The present invention relates to a composition for improving the condition of hair or scalp containing polysaccharides extracted from green tea,

본 발명은 인삼 다당체와 녹차 다당체를 함유하는 모발 또는 두피 상태 개선용 조성물에 관한 것으로서, 보다 상세하게는 포제 처리한 인삼 및 녹차의 추출물로부터 추출한 각각의 다당체를 혼합하여 사용함으로써 백모 및 탈모를 방지하며, 두피에 대한 자극을 완화하고 비듬, 두피 염증 등을 억제하여 전반적인 모발 상태를 개선시키고 두피에 대한 정화 및 진정 효과가 우수한 조성물에 대한 것이다.
The present invention relates to a hair or scalp condition improving composition containing a ginseng polysaccharide and a green tea polysaccharide. More particularly, the present invention relates to a composition for preventing hair loss and hair loss by mixing a polysaccharide extracted from an extract of green tea, , A composition which alleviates irritation to the scalp, suppresses dandruff and scalp inflammation, thereby improving the overall hair condition, and having a cleansing and soothing effect on the scalp.

두피는 표피(epidermis), 진피(dermis), 피하조직(subcutaneous tissue)으로 구성되어 있으며, 가장 바깥조직이 표피이다. 표피는 신체의 민감한 조직을 손상받지 않도록 보호해주는 기능을 한다. 그러나 외부의 온도, 습도의 변화, 공해물질 등의 여러 가지 물리적, 화학적 외부의 자극과 스트레스, 영향 결핍 등으로 인해 두피의 기능을 상실할 수 있으며, 나아가서는 탈모에도 영향을 미칠 수 있다. The scalp is composed of the epidermis, dermis, and subcutaneous tissue, and the outermost tissue is the epidermis. The epidermis protects the body's sensitive tissues from damage. However, due to various physical and chemical external stimuli such as external temperature, humidity change, pollutants, stress, and lack of influence, the scalp function may be lost, and furthermore, it may affect hair loss.

또한, 미용을 중시하는 현대사회에서는 개성표현 욕구를 충족시키기 위하여 남녀노소를 불문하고 많은 사람들이 염모제, 퍼머제 등을 사용하고 있다. 그러나, 일부 민감성 두피의 경우는 이러한 염색, 퍼머 등의 화학적 시술로 인해 두피의 가려움이나 따가움과 같은 자극현상과 염증이 유발되며, 이는 환경적 탈모로 이어지고 있다.In modern society, which emphasizes beauty, many people, both men and women, use hair dye, permanent hair, and the like in order to satisfy the need for expression of individuality. However, in the case of some sensitive scalp, chemical treatment such as dyeing and perming causes irritation such as itching or stinging of the scalp and inflammation, which leads to environmental hair loss.

이와 같이, 여러 가지 원인에 의한 환경으로부터의 자극, 스트레스, 화학적 시술 등으로부터 두피를 보호하고, 탈모를 방지하면서 모발 생장을 촉진할 수 있고, 생태계 친화적이면서 환경과 인간에 무해한 성분에 관한 연구가 지속되고 있다.
In this way, it is possible to protect the scalp from stimulation, stress, and chemical treatment from the environment due to various causes, to promote hair growth while preventing hair loss, and to continue research on ecosystem-friendly and environmentally harmless components to humans .

이에 본 발명자들은 인삼과 녹차를 포제 처리하고 이로부터 다당체를 추출하면, 포제 처리하지 않은 인삼 또는 녹차에 비하여 효능이 강화된 물질을 수득할 수 있으며, 이와 같이 얻은 인삼 다당체와 녹차 다당체를 동시에 사용하게 되면 상승 효과에 의하여 이들 다당체를 각각 사용하는 경우보다 백모 및 탈모 방지, 비듬 억제 등의 효과가 뛰어남을 발견하고 본 발명을 완성하게 되었다.Therefore, the inventors of the present invention have found that when ginseng and green tea are subjected to forage treatment and polysaccharide is extracted therefrom, a substance enhanced in potency can be obtained as compared with ginseng or green tea which has not been treated with faujasite, and the obtained ginseng polysaccharide and green tea polysaccharide are simultaneously used The present inventors have found that the polysaccharides are more effective than the polysaccharides for preventing white hair, hair loss, and dandruff, respectively, by the synergistic effect, and completed the present invention.

따라서, 본 발명은 포제 처리한 인삼 및 녹차 추출물로부터 추출한 인삼 다당체와 녹차 다당체를 동시에 사용함으로써 우수한 모발 또는 두피 상태 개선 효과를 나타내는 조성물을 제공하는 것을 목적으로 한다.
Accordingly, it is an object of the present invention to provide a composition exhibiting excellent hair or scalp condition improving effect by simultaneously using ginseng polysaccharide extracted from forage-treated ginseng and green tea extract and green tea polysaccharide.

상기한 목적을 달성하기 위하여, 본 발명은 포제 처리한 인삼 및 녹차의 추출물로부터 추출한 인삼 다당체와 녹차 다당체를 유효성분으로 함유하는 모발 또는 두피 상태 개선용 피부 외용제 조성물 또는 약학 조성물을 제공한다.
In order to achieve the above object, the present invention provides a composition for external application for skin or scalp or a pharmaceutical composition comprising the ginseng polysaccharide extracted from the extract of green tea and the green tea polysaccharide as an active ingredient.

본 발명의 조성물은 포제 처리한 인삼과 녹차의 추출물로부터 다당체를 추출함으로써 효능이 강화된 물질의 수득이 가능하며, 또한 인삼 다당체와 녹차 다당체를 동시에 함유함으로써 백모 및 탈모를 방지하고, 두피 자극을 완화시키며, 두피 가려움 및 비듬을 방지하는데 효과적일 뿐만 아니라, 모발을 더 부드럽고 윤기나게 한다.
The composition of the present invention can obtain a substance enhanced in efficacy by extracting a polysaccharide from a ginseng and green tea extract that has been subjected to forage treatment. It also contains ginseng polysaccharide and green tea polysaccharide simultaneously to prevent white hair and hair loss, Not only is it effective in preventing scalp itching and dandruff, it also makes the hair softer and shiny.

본 발명의 조성물은 유효성분으로서 인삼 다당체와 녹차 다당체를 함유하며, 상기 인삼 다당체와 녹차 다당체는 한방 가공 기술인 포제를 활용하여 가공한 인삼 및 녹차로부터 추출한다.The composition of the present invention contains a ginseng polysaccharide and a green tea polysaccharide as active ingredients, and the ginseng polysaccharide and the green tea polysaccharide are extracted from processed ginseng and green tea utilizing a herbicide processing technology.

본 발명에서 사용되는 인삼(Panax ginseng C.A. Meyer) 다당체는 분자량이 34,600인 펙틴(pectin) 유사물질로서, 갈락투론산(galacturonic acid)이 60% 정도를 차지하고, 그 외 아라비노스, 람노오스, 글루코스 및 갈락토오스 등이 측쇄를 구성하고 있다. 본 발명에서 사용되는 인삼은 오가피과 인삼 속에 속하는 식물로서, 본 발명에서 인삼은 그 종류 및 년근에 제한없이 사용가능하며, 말리거나 말리지 않은 인삼을 모두 사용할 수 있다. 또한 본 발명에서 인삼은 그 부위에 제한없이 사용 가능하며, 구체적으로는 인삼의 뿌리를 사용할 수 있다.The ginseng (Panax ginseng CA Meyer) polysaccharide used in the present invention is a pectin-like substance having a molecular weight of 34,600, and contains about 60% of galacturonic acid. In addition, arabinose, rhamnose, glucose and And galactose constitute side chains. The ginseng used in the present invention is a plant belonging to the genus Ogawa and Ginseng. In the present invention, ginseng can be used without limitation in its kind and year-round, and both dried and non-dried ginseng can be used. In the present invention, ginseng can be used without limitation in the site, and specifically, roots of ginseng can be used.

또한, 본 발명에서 사용되는 녹차(Green tea) 다당체는 녹차로부터 유래한 것으로서, 다른 식물에서 발견되는 다당체와는 달리 산성 다당체군이며, 광합성을 통해 만들어진 당 성분과 아미노산이 결합되어 생성된 것이다. 본 발명에서 녹차는 그 종류 및 재배 일수에 제한없이 사용가능하며, 말리거나 말리지 않은 녹차를 모두 사용할 수 있다. 또한 본 발명에서 녹차는 그 부위에 제한없이 사용 가능하며, 구체적으로는 녹차의 잎을 사용할 수 있다.The green tea polysaccharide used in the present invention is derived from green tea and is an acidic polysaccharide group, unlike a polysaccharide found in other plants, and is produced by combining sugar components and amino acids produced through photosynthesis. In the present invention, green tea can be used regardless of the type and the number of days of cultivation, and both dried and non-dried green tea can be used. In the present invention, the green tea can be used without limitation in the area, and specifically, the leaves of green tea can be used.

본 발명에서 사용되는 “포제(匍制)”는 한방이론에 근거하여 약재를 가공처리함으로써 약재 본래의 성질을 변화시키는 제약기술을 의미한다. 포제는 약재의 독성을 감소시키거나, 약재의 약성 보존, 약성 증진, 약재의 복용 편리성 증진을 위해 삶거나, 찌거나, 불에 볶거나, 불에 굽거나 달구는 과정을 포함할 수 있다. 아울러, 포제법에는, 예컨대, 약재를 볶는 초(炒)법, 약재를 일정량의 액체 보조재료와 함께 볶음으로써 보조재료가 약물조직 내에 스며들게 하는 자(炙)법, 약재 각 품목의 포제 규정에 따라 액체 보조재료를 넣고 섞어서 적당한 용기 안에서 가열하여 찌거나, 일정한 정도가 될 때까지 쪄서 말리는 증(蒸)법 등이 있다. As used herein, the term " formulated " means a constraint technique that changes the original properties of a medicament by processing the medicament based on the oriental theory. Foje can include boiling, steaming, roasting, roasting, burning, or boiling for the purpose of reducing the toxicity of the medicinal product, preserving the medicinal properties of the medicinal product, improving the medicinal properties, and improving the convenience of taking the medicinal product. In addition, the foaming method includes, for example, frying the herbal medicine, frying the medicinal material with a certain amount of the liquid auxiliary material, grinding the auxiliary material into the drug tissue, (Vapor) method in which a liquid auxiliary material is added and mixed, heated in a suitable container, and steamed or steamed until a certain degree is reached.

또한 포제법은 불의 세기, 사용하는 보료에 따라 더 세분화되는데, 그 몇 가지를 예로 들면 다음과 같다. 청초(淸炒)법은 문화(文火 : 불꽃이 약한 불) 또는 무화(武火 : 불꽃이 강한 불)로 규정된 정도가 될 때까지 약재를 볶는 방법이다. 주자(酒炙)법은 약 15%의 주정을 사용하여 약재를 볶는 방법이고, 주증(酒蒸)법은 약 15%의 주정을 사용하여 약재를 찌는 방법이다. 동일한 약용식물이라 하여도 생재와 숙재는 성질이 같지 않거나 작용에서 차이가 나는 경우가 있다. 또한 생재와 숙재를 적절히 조합한 복합처방은 각 약재의 약효를 서로 상승시키거나 독성을 상쇄하여 치료 효과를 극대화시킬 수 있다. In addition, the casting method is further subdivided according to the intensity of the fire and the type of the material to be used. Here are some examples. It is a method of frying medicinal materials until it reaches a level defined by culture (fire) or artificial fire (fire). The liquorice method is a method of roasting a medicinal substance using about 15% of the alcohol and the method of boiling the medicinal substance using about 15% of alcohol. Even in the same medicinal plant, the properties of the raw material and the sown material may not be the same or may differ from each other. In addition, the combined prescription of a combination of raw materials and mature materials can maximize the therapeutic effect by elevating the drug efficacy of each medicinal substance or by canceling the toxicity.

본 발명에서의 “포제 처리”는 상기 기술한 “포제”법에 의하여 추출물을 제조하기 전에 인삼 또는 녹차를 선처리하는 공정을 의미하며, 포제 처리를 통하여 기존에 인삼 또는 녹차에 없던 물질이 새로 생겨나거나, 기존 물질의 효능이 강화되는 효과를 얻을 수 있다. "Foaming treatment" in the present invention refers to a process of pre-treating ginseng or green tea prior to the preparation of the extract by the above-described "Foje" method. Through the foaming treatment, substances that were not present in ginseng or green tea are newly produced , The effect of enhancing the efficacy of the existing substance can be obtained.

포제 처리된 인삼 또는 녹차로부터 다당체를 추출하는 방법은 당업계에 공지된 방법을 이용할 수 있으며, 그 방법이 특별히 한정되지 않는다. A method known in the art can be used for the method of extracting the polysaccharide from the ginseng or green tea treated with fog, and the method is not particularly limited.

구체적으로, 38~42℃에서 물을 이용하여 ‘포제 처리한 인삼 또는 녹차’의 추출물을 제조한 후에 이를 농축하고, 이를 필터로 불순물을 제거한 다음, 여기에 에탄올을 적가하고 열풍 건조시킴으로써 녹차 다당체 및 인삼 다당체를 수득할 수 있다. Specifically, an extract of "ginseng or green tea" which has been treated with water at 38 to 42 ° C is prepared, and then concentrated. After removing impurities with a filter, ethanol is added dropwise thereto and the mixture is dried with hot air to obtain a green tea polysaccharide Ginseng polysaccharide can be obtained.

본 발명의 조성물은 인삼 다당체와 녹차 다당체를 각각 조성물 총 중량에 대하여 0.001~10중량%의 양으로 함유할 수 있으며, 바람직하게는 인삼 다당체와 녹차 다당체를 각각 조성물 총 중량에 대하여 0.005~9.5중량%, 0.01~9중량%, 0.03~8.5중량%, 0.05~8중량%, 0.07~7.5중량%, 0.09~7중량%, 0.1~6.5중량%, 0.3~6중량%, 0.5~5.5중량% 또는 0.7~5중량%의 양으로 함유할 수 있다.The composition of the present invention may contain the ginseng polysaccharide and the green tea polysaccharide in an amount of 0.001 to 10% by weight based on the total weight of the composition, preferably the ginseng polysaccharide and the green tea polysaccharide in an amount of 0.005 to 9.5% 0.09 to 7.5 wt.%, 0.1 to 6.5 wt.%, 0.3 to 6 wt.%, 0.5 to 5.5 wt.%, Or 0.7 By weight to 5% by weight.

본 발명에 있어서, 인삼 다당체와 녹차 다당체는 중량비로 0.1~10:1의 범위로 혼합하여 사용된다. 또한, 상기 두 성분의 병용에 따른 상승 효과를 극대화하기 위하여, 바람직하게는 인삼 다당체와 녹차 다당체를 중량비로 0.5~9.5:1, 1~9:1 또는 1.5~8.5:1의 범위로 혼합하여 사용할 수 있다. In the present invention, the ginseng polysaccharide and the green tea polysaccharide are mixed in a weight ratio of 0.1 to 10: 1. In order to maximize the synergistic effect of the combination of the two components, preferably, the ginseng polysaccharide and the green tea polysaccharide are mixed in a weight ratio of 0.5 to 9.5: 1, 1 to 9: 1, or 1.5 to 8.5: 1 .

본 발명의 조성물은 비듬 방지용 조성물로서 사용될 수 있으며, 이는 모발과 두피에 축적된 독소를 효과적으로 배출하여 두피를 정화하고, 비듬균의 증식과 성장을 억제하여 두피염증반응을 예방할 수 있으며, 또한 활성산소의 생성 및 작용을 억제하는 항산화 효능이 뛰어나 두피를 진정시키고 강화시키며 본연의 방어력을 강화시키는 효과를 제공할 수 있다.The composition of the present invention can be used as a composition for preventing dandruff. It can purify the scalp by effectively discharging toxins accumulated in hair and scalp, inhibit proliferation and growth of dandruff and prevent scalp inflammation reaction, It has excellent antioxidant ability to inhibit the production and action, so it can calm and strengthen the scalp and provide the effect of strengthening the original defense.

본 발명의 조성물은 육모용 조성물로서 사용될 수 있으며, 이는 휴지기 모발주기의 성장기 모발주기로의 이행을 촉진시킴으로써 모발의 생장을 촉진하고, 탈모를 방지하는 효과를 제공할 수 있다.The composition of the present invention can be used as a hair tonic composition, which promotes the transition of the resting hair cycle to the growing hair cycle, thereby promoting hair growth and preventing hair loss.

본 발명의 조성물은 백모 방지용 피부 외용제 조성물로서 사용될 수 있으며, 이는 멜라노사이트에서 MITF의 발현을 현저히 상승시켜 백모를 억제하며 흑모 유발을 촉진시킬 수 있다.The composition of the present invention can be used as a composition for external application for skin preventing anti-whiplash, which can significantly increase the expression of MITF in melanocytes, thereby inhibiting white moth and promoting gray hair induction.

본 발명의 조성물은 두피의 가려움, 따가움 등의 자극 현상을 감소시키고 두피 염증을 완화하여 진정시킴으로써 민감성, 자극성 또는 반응성 두피를 안정화시킬 수 있다. 또한, 거칠어지고 손상된 모발을 부드럽고 매끄럽게 해주는 컨디셔닝 효과도 제공함으로써 모발 상태를 건강하게 개선시킬 수 있다.The composition of the present invention can stabilize a sensitive, irritating or reactive scalp by reducing irritation such as itching and stinging of the scalp and alleviating and alleviating the scalp inflammation. In addition, hair condition can be improved healthily by providing a conditioning effect that makes rough and damaged hair soft and smooth.

본 발명의 조성물은 피부 외용제 조성물, 특히 화장료 조성물로서 제형화될 수 있으며, 화장품학 또는 피부과학적으로 허용 가능한 매질 또는 기제를 함유하여 제형화될 수 있다. 또한, 본 발명의 조성물은 국소 적용에 적합한 모든 제형으로 제공될 수 있으며, 예를 들어 용액, 수상에 유상을 분산시켜 얻은 에멀젼, 유상에 수상을 분산시켜 얻은 에멀젼, 현탁액, 고체, 겔, 분말, 페이스트, 포말(foam) 또는 에어로졸 조성물의 제형으로 제공될 수 있다. 이러한 제형의 조성물은 당해 분야의 통상적인 방법에 따라 제조될 수 있다.The composition of the present invention may be formulated as an external preparation for skin, in particular as a cosmetic composition, and may be formulated containing a cosmetically or dermatologically acceptable medium or base. In addition, the composition of the present invention may be provided in any form suitable for topical application, for example, as a solution, an emulsion obtained by dispersing an oil phase in water phase, an emulsion obtained by dispersing water phase in water phase, a suspension, a solid, a gel, Pastes, foams, or aerosol compositions. Compositions of such formulations may be prepared according to conventional methods in the art.

특히, 본 발명의 피부 외용제 조성물은 모발 또는 두피용 조성물로서 제형이 특별히 한정되는 것은 아니지만, 예를 들어 헤어토닉, 모발 영양화장수, 스칼프트리트먼트, 헤어트리트먼트, 헤어샴푸, 헤어린스, 헤어로션 또는 두피 모발 겸용 트리트먼트 등으로 제형화될 수 있다.In particular, the composition for external application for skin of the present invention is not particularly limited as a composition for hair or scalp. For example, hair tonic, hair nutritional lotion, scalp treatment, hair treatment, hair shampoo, hair rinse, Scalp hair combined treatment, and the like.

또한, 본 발명에 따른 조성물은 상기한 물질 이외에 주 효과를 손상시키지 않는 범위 내에서, 바람직하게는 주 효과에 상승 효과를 줄 수 있는 다른 성분들을 포함할 수 있다. 또한 본 발명에 따른 조성물은 보습제, 에몰리언트제, 자외선 흡수제, 방부제, 살균제, 산화 방지제, pH 조정제, 유기 및 무기 안료, 향료, 냉감제 또는 제한제를 더 포함할 수 있다. 상기 성분의 배합량은 본 발명의 목적 및 효과를 손상시키지 않는 범위 내에서 당업자가 용이하게 선정 가능하며, 그 배합량은 조성물 총 중량에 대하여 0.01~5중량%, 구체적으로 0.01~3중량%일 수 있다.In addition, the composition according to the present invention may contain, in addition to the above-mentioned substances, other ingredients which can give a synergistic effect to the main effect, to the extent that the main effect is not impaired. The composition according to the present invention may further comprise a humectant, an emollient, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an organic or inorganic pigment, a perfume, a cold agent or a limiting agent. The compounding amount of the above components can be easily selected by a person skilled in the art within the range not impairing the object and effect of the present invention, and the amount thereof may be from 0.01 to 5% by weight, specifically from 0.01 to 3% by weight based on the total weight of the composition .

또한, 본 발명의 조성물은 약학 조성물로서 제형화될 수 있다. 본 발명에 따른 조성물을 의약품에 적용할 경우에는, 본 발명에서 사용되는 유효성분에 상용되는 무기 또는 유기의 담체를 가하여 고체, 반고체 또는 액상의 형태로 경구 투여제 혹은 비경구 투여제로 제형화할 수 있으며, 본 발명에 따른 상기 약학 조성물은 경구, 비경구, 직장, 국소, 경피, 정맥 내, 근육 내, 복강 내, 피하 등으로 투여될 수 있다. In addition, the composition of the present invention can be formulated as a pharmaceutical composition. When the composition according to the present invention is applied to medicines, it may be formulated into oral, parenteral or parenteral dosage forms in solid, semi-solid or liquid form by adding an inorganic or organic carrier compatible with the active ingredient used in the present invention , The pharmaceutical composition according to the present invention may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously, and the like.

상기 경구 투여를 위한 제형으로서는 정제, 환제, 과립제, 캡슐제, 산제, 세립제, 분제, 유탁제, 시럽제, 펠렛제 등을 들 수 있다. 또한, 상기 비경구 투여를 위한 제형으로는 주사제, 점적제, 연고, 로션, 스프레이, 현탁제, 유제, 좌제 등을 들 수 있다. 본 발명에 따른 조성물은 상법에 따라 실시함으로써, 유효성분을 용이하게 제형화할 수 있으며, 이 때 계면활성제, 부형제, 착색료, 향신료, 보존료, 안정제, 완충제, 현탁제, 기타 상용하는 보조제를 적당히 사용할 수 있다.Examples of the formulations for oral administration include tablets, pills, granules, capsules, powders, fine granules, powders, emulsions, syrups and pellets. In addition, the formulations for parenteral administration include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. The composition according to the present invention can be easily formulated into an active ingredient by carrying out the composition according to a conventional method. In this case, a surfactant, excipient, coloring agent, spice, preservative, stabilizer, buffer, suspending agent, have.

본 발명의 약학 조성물의 유효 성분의 투여 용량은 투여받을 대상의 연령, 성별, 체중, 병리 상태 및 그 심각도, 투여 경로 또는 처방자의 판단에 따라 달라질 것이다. 이러한 인자에 기초한 적당한 용량의 결정은 당업자의 수준 내에 있으며, 이의 1일 투여 용량은 예를 들어 0.1mg/kg/일 내지 100mg/kg/일, 보다 구체적으로는 5mg/kg/일 내지 50mg/kg/일이 될 수 있으나, 이에 제한되는 것은 아니다.
The dosage of the active ingredient of the pharmaceutical composition of the present invention will vary depending on the age, sex, body weight, pathological condition and severity of the subject to be treated, route of administration, or judgment of the prescriber. Determination of the appropriate dose based on these factors is well within the level of ordinary skill in the art and its daily dose is, for example, from 0.1 mg / kg / day to 100 mg / kg / day, more specifically from 5 mg / kg / day to 50 mg / / Day, but is not limited thereto.

이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 예시하기 위한 것으로, 본 발명의 범위가 이들 실시예에 의해 제한되는 것으로 해석되지 않는 것은 당업계에서 통상의 지식을 가진 자에게 있어서 자명할 것이다.
Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for illustrating the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

[비교예 1-1] 증법포제 인삼 다당체의 제조[Comparative Example 1-1] Preparation of Forsythia ginseng polysaccharide

건조하지 않은 수삼을 고압증숙기에 넣고 95~100℃ 온도로 약 3~4시간 동안 고압 증숙한 다음, 상기 공정 단계를 완료한 증숙된 수삼을 열풍건조기에서 60~70℃의 온도로 건조시켰다. 증법포제로 가공된 인삼 1kg을 믹서기로 분쇄한 뒤 정제수 10ℓ를 넣고, 38~42℃ 온수로 24시간 교반 추출한 다음, 15℃에서 1일간 침적시켰다. 그 후, 여과포 여과와 원심분리를 통해 잔사와 여액을 분리하고, 분리된 여액을 정제수 양의 1/10까지 감압 농축하였다. 여기에 농축된 양의 4배수의 에탄올을 적가하고 열풍 건조시켜 포제 한방 가공 기술인 포제를 활용하여 증법 가공된 인삼 다당체 70g을 얻었다.
The non-dried ginseng was put into a high pressure steam boiler and steamed at 95 to 100 ° C for about 3 to 4 hours under high pressure. Then, the steamed ginseng that had completed the above process steps was dried at 60 to 70 ° C in a hot air dryer. 1 kg of ginseng processed with forage preparation was pulverized with a blender, and 10 L of purified water was added thereto. The resulting mixture was stirred for 24 hours at 38 to 42 ° C in hot water, and then immersed at 15 ° C for 1 day. Thereafter, the residue and the filtrate were separated by filtration and centrifugation through a filter cloth, and the separated filtrate was concentrated under reduced pressure to 1/10 of the amount of purified water. Four times as much concentrated ethanol as the concentrated amount was added dropwise, and hot air drying was conducted to obtain 70 g of the ginseng polysaccharide produced by the foojyeong processing technique.

[비교예 1-2] 증법포제 녹차 다당체의 제조[Comparative Example 1-2] Preparation of fugitive green tea polysaccharide

1차 가공된(덖음 과정) 녹차 잎을 사용하는 것을 제외하고는 상기 비교예 1-1과 동일한 방법을 사용하여 증법 가공된 녹차 다당체 70g을 얻었다.
70 g of a green tea polysaccharide processed by the same method as in Comparative Example 1-1 was obtained, except that the green tea leaves were used in the first processed (roughening process).

[실시예 1-1] 증법포제 인삼 및 녹차 다당체의 제조(1:3의 중량비로 혼합)[Example 1-1] Preparation of booster ginseng and green tea polysaccharide (mixed at a weight ratio of 1: 3)

건조하지 않은 수삼 25중량%와 1차 가공된(덖음 과정) 녹차 잎 75중량%를 고압증숙기에 넣고 95~100℃ 온도로 약 3~4시간 동안 고압 증숙한 다음, 상기 공정 단계를 완료한 증숙된 수삼을 열풍건조기에서 60~70℃의 온도로 건조시켰다. 증법포제로 가공된 인삼 및 녹차 혼합물 1kg을 믹서기로 분쇄한 뒤 정제수 10ℓ를 넣고, 38~42℃ 온수로 24시간 교반 추출한 다음, 15℃에서 1일간 침적시켰다. 그 후, 여과포 여과와 원심분리를 통해 잔사와 여액을 분리하고, 분리된 여액을 정제수 양의 1/10까지 감압 농축하였다. 여기에 농축된 양의 4배수의 에탄올을 적가하고 열풍 건조시켜 포제 한방 가공 기술인 포제를 활용하여 증법 가공된 인삼 다당체와 녹차 다당체 혼합물 78g을 얻었다.
25% by weight of non-dried ginseng and 75% by weight of primary processed green tea leaves (75% by weight) were added to a high-pressure tiller and heated at 95 to 100 ° C for about 3 to 4 hours under high pressure. The dried ginseng was dried in a hot air dryer at a temperature of 60 to 70 ° C. 1 kg of a mixture of ginseng and green tea processed by forging process was pulverized with a blender, and 10 L of purified water was added thereto. The mixture was stirred at 38 to 42 캜 in hot water for 24 hours and then immersed at 15 캜 for 1 day. Thereafter, the residue and the filtrate were separated by filtration and centrifugation through a filter cloth, and the separated filtrate was concentrated under reduced pressure to 1/10 of the amount of purified water. Then, ethanol was added dropwise to a concentrated amount of ethanol, and the mixture was subjected to hot air drying to obtain 78 g of a mixture of ginseng polysaccharide and green tea polysaccharide,

[실시예 1-2] 증법포제 인삼 및 녹차 다당체의 제조(1:1의 중량비로 혼합)[Example 1-2] Preparation of booster ginseng and green tea polysaccharide (mixed at a weight ratio of 1: 1)

건조하지 않은 수삼 50중량%와 1차 가공된(덖음 과정) 녹차 잎 50중량%를 사용하는 것을 제외하고는 상기 실시예 1-1과 동일한 방법을 사용하여 증법 가공된 인삼 다당체와 녹차 다당체 혼합물 75g을 얻었다.
A mixture of ginseng polysaccharide and green tea polysaccharide prepared by the same method as in Example 1-1 except that 50 wt% of non-dried ginseng and 50 wt% of green tea leaves of the first processed ≪ / RTI >

[실시예 1-3] 증법포제 인삼 및 녹차 다당체의 제조(3:1의 중량비로 혼합)[Example 1-3] Preparation of booster ginseng and green tea polysaccharide (mixed at a weight ratio of 3: 1)

건조하지 않은 수삼 75중량%와 1차 가공된(덖음 과정) 녹차 잎 25중량%를 사용하는 것을 제외하고는 상기 실시예 1-1과 동일한 방법을 사용하여 증법 가공된 인삼 다당체와 녹차 다당체 혼합물 70g을 얻었다.
A mixture of ginseng polysaccharide and green tea polysaccharide prepared by the same method as in Example 1-1 except that 75% by weight of non-dried ginseng and 25% by weight of first-processed ≪ / RTI >

[비교예 2-1] 인삼 염자(鹽炙) 다당체의 제조[Comparative Example 2-1] Preparation of polysaccharide of ginseng (salt)

건조한 인삼을 소금물(2~3%)과 잘 혼합시킨 후 밀폐시켜 완전히 흡수 되도록 방치하고, 소금물이 완전히 흡수된 인삼을 100~180℃에서 초제 용기에 넣은 다음 10분~1시간 동안 볶은 후 그늘에 말렸다. 이렇게 말린 인삼에 정제수 10ℓ를 넣고, 3회 환류 추출한 다음, 15℃에서 1일간 침적시켰다. 그 후, 여과포 여과와 원심분리를 통해 잔사와 여액을 분리하고, 분리된 여액을 정제수 양의 1/10까지 감압 농축하였다. 여기에 농축된 양의 4배수의 에탄올을 적가하고 열풍 건조시켜 염자 가공된 인삼 다당체 73g을 얻었다.
Dry ginseng is mixed well with salt water (2 ~ 3%), sealed and left to be completely absorbed. Ginseng, which is completely absorbed in salt water, is put into a supergiant vessel at 100 ~ 180 ℃ and roasted for 10 minutes ~ 1 hour. I was dry. 10 L of purified water was added to the dried ginseng, refluxed three times, and then immersed at 15 캜 for one day. Thereafter, the residue and the filtrate were separated by filtration and centrifugation through a filter cloth, and the separated filtrate was concentrated under reduced pressure to 1/10 of the amount of purified water. The concentrated amount of ethanol was added dropwise thereto, and the mixture was hot-air dried to obtain 73 g of a ginseng-treated polysaccharide.

[비교예 2-2] 녹차 염자 다당체의 제조[Comparative Example 2-2] Production of green tea polysaccharide

1차 가공된(덖음 과정) 녹차 잎을 사용하는 것을 제외하고는 상기 비교예 2-1과 동일한 방법을 사용하여 염자 가공된 녹차 다당체 76g을 얻었다.
76 g of a green tea polysaccharide obtained by the same procedure as in Comparative Example 2-1 was obtained, except that the green tea leaves were used in the primary treatment (smoothing process).

[실시예 2-1] 인삼 및 녹차 염자 다당체의 제조(1:3의 중량비로 혼합)[Example 2-1] Production of ginseng and green tea polysaccharide (mixed at a weight ratio of 1: 3)

건조한 인삼 25중량%와 1차 가공된(덖음 과정) 녹차 잎 75중량%를 사용하는 것을 제외하고는 상기 비교예 2-1과 동일한 방법을 사용하여 염자 가공된 인삼 다당체와 녹차 다당체 혼합물 72g을 얻었다.
72 g of a ginseng polysaccharide and a green tea polysaccharide mixture were obtained in the same manner as in Comparative Example 2-1, except that 25% by weight of dried ginseng and 75% by weight of first-processed .

[실시예 2-2] 인삼 및 녹차 염자 다당체의 제조(1:1의 중량비로 혼합)[Example 2-2] Preparation of ginseng and green tea polysaccharide (mixed at a weight ratio of 1: 1)

건조한 인삼 50중량%와 1차 가공된(덖음 과정) 녹차 잎 50중량%를 사용하는 것을 제외하고는 상기 비교예 2-1과 동일한 방법을 사용하여 염자 가공된 인삼 다당체와 녹차 다당체 혼합물 70g을 얻었다.
70 g of a ginseng polysaccharide and a green tea polysaccharide mixture were obtained by using the same method as in Comparative Example 2-1, except that 50% by weight of dried ginseng and 50% by weight of first-processed .

[실시예 2-3] 인삼 및 녹차 염자 다당체의 제조(3:1의 중량비로 혼합)[Example 2-3] Preparation of ginseng and green tea polysaccharide (mixed at a weight ratio of 3: 1)

건조한 인삼 75중량%와 1차 가공된(덖음 과정) 녹차 잎 25중량%를 사용하는 것을 제외하고는 상기 비교예 2-1과 동일한 방법을 사용하여 염자 가공된 인삼 다당체와 녹차 다당체 혼합물 75g을 얻었다.
75 g of a ginseng polysaccharide and a green tea polysaccharide mixture were obtained by using the same method as in Comparative Example 2-1, except that 75% by weight of dried ginseng and 25% by weight of first-processed .

[비교예 3-1] 인삼 초탄(炒炭) 다당체의 제조[Comparative Example 3-1] Production of polysaccharide of ginseng basil (roasted charcoal)

건조한 인삼을 230~300℃에서 외부를 탄화시켜 흑색이 되면 가열을 중지하고 상온에서 식혀 이렇게 얻은 인삼 1kg에 정제수 10ℓ를 넣은 후, 3회 환류 추출한 다음, 15℃에서 1일간 침적시켰다. 그 후, 여과포 여과와 원심분리를 통해 잔사와 여액을 정제수 양의 1/10까지 감압 농축하였다. 여기에 농축된 양의 4배수의 에탄올을 적가하고 열풍 건조시켜 초탄 가공된 인삼 다당체 72g을 얻었다.
The dried ginseng was carbonized outside at 230 to 300 ° C. When it turned black, the heating was stopped. The mixture was cooled at room temperature. To 1 kg of ginseng thus obtained, 10 L of purified water was added, refluxed three times and then immersed at 15 ° C for 1 day. Thereafter, the filtrate and the filtrate were concentrated under reduced pressure to 1/10 of the amount of purified water through filtration of the filter cloth and centrifugation. Four times as much concentrated ethanol as the concentrated amount was added dropwise, and the mixture was hot-air dried to obtain 72 g of a ginseng polysaccharide which had been subjected to super-calcination.

[비교예 3-2] 녹차 초탄 다당체의 제조[Comparative Example 3-2] Production of green tea chitosan polysaccharide

1차 가공된(덖음 과정) 녹차 잎을 사용하는 것을 제외하고는 상기 비교예 3-1과 동일한 방법을 사용하여 초탄 가공된 녹차 다당체 72g을 얻었다.
A green tea polysaccharide (72 g) was obtained by the same method as in Comparative Example 3-1, except that the green tea leaves were used in the primary treatment (smoothing process).

[실시예 3-1] 인삼 및 녹차 초탄 다당체의 제조(1:3의 중량비로 혼합)[Example 3-1] Production of ginseng and tea green tea polysaccharide (mixed at a weight ratio of 1: 3)

건조한 인삼 25중량%와 1차 가공된(덖음 과정) 녹차 잎 75중량%를 사용하는 것을 제외하고는 상기 비교예 3-1과 동일한 방법을 사용하여 초탄 가공된 인삼 다당체와 녹차 다당체 혼합물 75g을 얻었다.
75 g of a ginseng polysaccharide and a green tea polysaccharide mixture were obtained by the same method as in Comparative Example 3-1, except that 25% by weight of dried ginseng and 75% by weight of first-processed (smoothing process) green tea leaves were used .

[실시예 3-2] 인삼 및 녹차 초탄 다당체의 제조(1:1의 중량비로 혼합)[Example 3-2] Production of ginseng and tea green tea polysaccharide (mixed at a weight ratio of 1: 1)

건조한 인삼 50중량%와 1차 가공된(덖음 과정) 녹차 잎 50중량%를 사용하는 것을 제외하고는 상기 비교예 3-1과 동일한 방법을 사용하여 초탄 가공된 인삼 다당체와 녹차 다당체 혼합물 71g을 얻었다.
Using the same method as in Comparative Example 3-1, 71 g of a ginseng polysaccharide and a green tea polysaccharide mixture were obtained, except that 50% by weight of dried ginseng and 50% by weight of primary processed (roasted) green tea leaves were used .

[실시예 3-3] 인삼 및 녹차 초탄 다당체의 제조(3:1의 중량비로 혼합)[Example 3-3] Preparation of ginseng and tea green tea polysaccharide (mixed at a weight ratio of 3: 1)

건조한 인삼 75중량%와 1차 가공된(덖음 과정) 녹차 잎 25중량%를 사용하는 것을 제외하고는 상기 비교예 3-1과 동일한 방법을 사용하여 초탄 가공된 인삼 다당체와 녹차 다당체 혼합물 70g을 얻었다.
70 g of a ginseng polysaccharide and a green tea polysaccharide mixture were obtained by the same method as in Comparative Example 3-1, except that 75% by weight of dried ginseng and 25% by weight of primary processed green tea leaves were used .

[비교예 4-1] 인삼 초자(醋炙) 다당체의 제조[Comparative Example 4-1] Preparation of ginseng horseradish polysaccharide

건조한 인삼에 식초 200~300g을 충분히 흡수시킨 후 100~160℃에서 10분~1시간 동안 볶은 후 그늘에 말렸다. 이렇게 얻은 인삼 1kg에 정제수 10ℓ를 넣고, 3회 환류 추출한 다음, 15℃에서 1일간 침적시켰다. 그 후, 여과포 여과와 원심분리를 통해 잔사와 여액을 정제수 양의 1/10까지 감압 농축하였다. 여기에 농축된 양의 4배수의 에탄올을 적가하고 열풍 건조시켜 초자 가공된 인삼 다당체 71g을 얻었다.
200-300 g of vinegar was sufficiently absorbed in dried ginseng and roasted at 100 ~ 160 ℃ for 10 minutes ~ 1 hour and dried in the shade. To 1 kg of the thus-obtained ginseng was added 10 L of purified water, refluxed three times, and then immersed at 15 캜 for 1 day. Thereafter, the filtrate and the filtrate were concentrated under reduced pressure to 1/10 of the amount of purified water through filtration of the filter cloth and centrifugation. Four times as much concentrated ethanol as the concentrated amount was added dropwise, and the mixture was hot-air dried to obtain 71 g of a gum polysaccharide prepared by processing.

[비교예 4-2] 녹차 초자 다당체의 제조[Comparative Example 4-2] Production of green tea hyaluronic acid polysaccharide

1차 가공된(덖음 과정) 녹차 잎을 사용하는 것을 제외하고는 상기 비교예 4-1과 동일한 방법을 사용하여 초자 가공된 녹차 다당체 73g을 얻었다.
73 g of green tea polysaccharide prepared in the same manner as in Comparative Example 4-1 was obtained, except that green tea leaves were used in the first processed (roughening process).

[실시예 4-1] 인삼 및 녹차 초자 다당체의 제조(1:3의 중량비로 혼합)[Example 4-1] Preparation of ginseng and tea green tea polysaccharide (mixed at a weight ratio of 1: 3)

건조한 인삼 25중량%와 1차 가공된(덖음 과정) 녹차 잎 75중량%를 사용하는 것을 제외하고는 상기 비교예 4-1과 동일한 방법을 사용하여 초자 가공된 인삼 다당체와 녹차 다당체 혼합물 76g을 얻었다.
Using the same method as in Comparative Example 4-1, 76 g of a mixture of green ginseng polysaccharide and green tea polysaccharide was obtained, except that 25% by weight of dry ginseng and 75% by weight of first-processed (smoothing process) green tea leaves were used .

[실시예 4-2] 인삼 및 녹차 초자 다당체의 제조(1:1의 중량비로 혼합)[Example 4-2] Preparation of ginseng and tea green tea polysaccharide (mixed at a weight ratio of 1: 1)

건조한 인삼 50중량%와 1차 가공된(덖음 과정) 녹차 잎 50중량%를 사용하는 것을 제외하고는 상기 비교예 4-1과 동일한 방법을 사용하여 초자 가공된 인삼 다당체와 녹차 다당체 혼합물 74g을 얻었다.
74 g of a green ginseng polysaccharide mixture and a green tea polysaccharide mixture were obtained in the same manner as in Comparative Example 4-1, except that 50% by weight of dried ginseng and 50% by weight of primary processed green tea leaves were used .

[실시예 4-3] 인삼 및 녹차 초자 다당체의 제조(3:1의 중량비로 혼합)[Example 4-3] Preparation of ginseng and tea green tea polysaccharide (mixed at a weight ratio of 3: 1)

건조한 인삼 75중량%와 1차 가공된(덖음 과정) 녹차 잎 25중량%를 사용하는 것을 제외하고는 상기 비교예 4-1과 동일한 방법을 사용하여 초자 가공된 인삼 다당체와 녹차 다당체 혼합물 74g을 얻었다.
Using the same method as in Comparative Example 4-1, 74 g of a mixture of green ginseng polysaccharide and green tea polysaccharide was obtained, except that 75% by weight of dried ginseng and 25% by weight of primary processed green tea leaves were used .

[비교예 5-1] 인삼 주증(酒蒸) 다당체의 제조[Comparative Example 5-1] Preparation of ginseng saponin polysaccharide

건조한 인삼을 일정량의 황주에 축축해지도록 담가 두고 이것을 찜기에 담아 30분~2시간 동안 찐 다음 그늘에 말렸다. 이렇게 얻은 인삼 1kg에 정제수 10ℓ를 넣고, 3회 환류 추출한 다음, 15℃에서 1일간 침적시켰다. 그 후, 여과포 여과와 원심분리를 통해 잔사와 여액을 정제수 양의 1/10까지 감압 농축하였다. 여기에 농축된 양의 4배수의 에탄올을 적가하고 열풍 건조시켜 주증 가공된 인삼 다당체 77g을 얻었다.
Dried ginseng was soaked in a certain amount of sulfur, soaked in a steamer, steamed for 30 minutes to 2 hours, and then dried in the shade. To 1 kg of the thus-obtained ginseng was added 10 L of purified water, refluxed three times, and then immersed at 15 캜 for 1 day. Thereafter, the filtrate and the filtrate were concentrated under reduced pressure to 1/10 of the amount of purified water through filtration of the filter cloth and centrifugation. Four times as much concentrated ethanol as the concentrated amount was added dropwise, and the mixture was hot-air dried to obtain 77 g of a ginseng polysaccharide obtained by corn processing.

[비교예 5-2] 녹차 주증 다당체의 제조[Comparative Example 5-2] Preparation of green tea vernal polysaccharide

1차 가공된(덖음 과정) 녹차 잎을 사용하는 것을 제외하고는 상기 비교예 5-1과 동일한 방법을 사용하여 주증 가공된 녹차 다당체 75g을 얻었다.
75 g of cornified green tea polysaccharide was obtained in the same manner as in Comparative Example 5-1, except that the green tea leaves were used for the first-time processing (smoothing process).

[실시예 5-1] 인삼 및 녹차 주증 다당체의 제조(1:3의 중량비로 혼합)[Example 5-1] Production of ginseng and tea green tea polysaccharide (mixed at a weight ratio of 1: 3)

건조한 인삼 25중량%와 1차 가공된(덖음 과정) 녹차 잎 75중량%를 사용하는 것을 제외하고는 상기 비교예 5-1과 동일한 방법을 사용하여 주증 가공된 인삼 다당체와 녹차 다당체 혼합물 74g을 얻었다.
Using the same method as in Comparative Example 5-1, 74 g of a mixture of ginseng polysaccharide and green tea polysaccharide was obtained, except that 25% by weight of dry ginseng and 75% by weight of primary processed green tea leaves were used .

[실시예 5-2] 인삼 및 녹차 초자 다당체의 제조(1:1의 중량비로 혼합)[Example 5-2] Preparation of ginseng and tea green tea polysaccharide (mixed at a weight ratio of 1: 1)

건조한 인삼 50중량%와 1차 가공된(덖음 과정) 녹차 잎 50중량%를 사용하는 것을 제외하고는 상기 비교예 5-1과 동일한 방법을 사용하여 주증 가공된 인삼 다당체와 녹차 다당체 혼합물 77g을 얻었다.
77 g of a ginseng polysaccharide mixture and a green tea polysaccharide mixture were obtained by using the same method as in Comparative Example 5-1 except that 50% by weight of dried ginseng and 50% by weight of first-processed .

[실시예 5-3] 인삼 및 녹차 주증 다당체의 제조(3:1의 중량비로 혼합)[Example 5-3] Preparation of ginseng and tea green tea polysaccharide (mixed at a weight ratio of 3: 1)

건조한 인삼 75중량%와 1차 가공된(덖음 과정) 녹차 잎 25중량%를 사용하는 것을 제외하고는 상기 비교예 5-1과 동일한 방법을 사용하여 주증 가공된 인삼 다당체와 녹차 다당체 혼합물 77g을 얻었다.
77 g of a ginseng polysaccharide mixture and a green tea polysaccharide mixture were obtained by using the same method as in Comparative Example 5-1 except that 75% by weight of dried ginseng and 25% by weight of first-processed .

[비교예 6-1] 인삼 강자(薑炙) 다당체의 제조[Comparative Example 6-1] Preparation of ginseng fermented polysaccharide

신선한 생강을 마쇄한 후 두배의 물을 넣어 혼합하여 압착한뒤 즙을 짜내었다. 이를 2~3회 반복하여 수행하여 생강즙을 준비하고, 건조한 인삼에 인삼 중량 대비 10~15%의 생강즙을 뿌려 골고루 축인 후 생강즙이 스며들면 100~180℃에서 용기에 넣고 10분~1시간 동안 볶은 후 그늘에 말렸다. 이렇게 얻은 인삼 1kg에 정제수 10ℓ를 넣고, 3회 환류 추출한 다음, 15℃에서 1일간 침적시켰다. 그 후, 여과포 여과와 원심분리를 통해 잔사와 여액을 정제수 양의 1/10까지 감압 농축하였다. 여기에 농축된 양의 4배수의 에탄올을 적가하고 열풍 건조시켜 강자 가공된 인삼 다당체 69g을 얻었다.
Fresh ginger was crushed, and then double water was added to the mixture. The mixture was squeezed and squeezed. Ginger juice is prepared by repeating 2 ~ 3 times to prepare ginger juice. Spread ginger juice of 10 ~ 15% of ginseng with respect to the weight of ginseng in dried ginseng, and then, stirring ginger juice, it is put into a container at 100 ~ 180 캜 for 10 ~ Then I dried it in the shade. To 1 kg of the thus-obtained ginseng was added 10 L of purified water, refluxed three times, and then immersed at 15 캜 for 1 day. Thereafter, the filtrate and the filtrate were concentrated under reduced pressure to 1/10 of the amount of purified water through filtration of the filter cloth and centrifugation. Four times as much ethanol as the concentrated amount was added dropwise, and the mixture was hot-air dried to obtain 69 g of a strong-processed ginseng polysaccharide.

[비교예 6-2] 녹차 강자 다당체의 제조[Comparative Example 6-2] Preparation of green tea powder polysaccharide

1차 가공된(덖음 과정) 녹차 잎을 사용하는 것을 제외하고는 상기 비교예 6-1과 동일한 방법을 사용하여 강자 가공된 녹차 다당체 73g을 얻었다.
73 g of a green tea polysaccharide prepared by the same method as in Comparative Example 6-1 was obtained, except that green tea leaves were used in the first processed (roughening process).

[실시예 6-1] 인삼 및 녹차 강자 다당체의 제조(1:3의 중량비로 혼합)[Example 6-1] Preparation of ginseng and green tea strong polysaccharide (mixed at a weight ratio of 1: 3)

인삼 25중량%와 1차 가공된(덖음 과정) 녹차 잎 75중량%를 사용하는 것을 제외하고는 상기 비교예 6-1과 동일한 방법을 사용하여 강자 가공된 인삼 다당체와 녹차 다당체 혼합물 71g을 얻었다.
71 g of a mixture of a ginseng polysaccharide and a green tea polysaccharide was prepared by using the same method as in Comparative Example 6-1, except that 25% by weight of ginseng and 75% by weight of the first processed (roasted) green tea leaves were used.

[실시예 6-2] 인삼 및 녹차 강자 다당체의 제조(1:1의 중량비로 혼합)[Example 6-2] Preparation of ginseng and green tea strong polysaccharide (mixed at a weight ratio of 1: 1)

인삼 50중량%와 1차 가공된(덖음 과정) 녹차 잎 50중량%를 사용하는 것을 제외하고는 상기 비교예 6-1과 동일한 방법을 사용하여 강자 가공된 인삼 다당체와 녹차 다당체 혼합물 70g을 얻었다.
Ginseng polysaccharide and 70 g of a green tea polysaccharide mixture were obtained by using the same method as in Comparative Example 6-1, except that 50% by weight of ginseng and 50% by weight of green tea leaves of the first processed (roasted) were used.

[실시예 6-3] 인삼 및 녹차 강자 다당체의 제조(3:1의 중량비로 혼합)[Example 6-3] Preparation of ginseng and green tea strong polysaccharide (mixed at a weight ratio of 3: 1)

인삼 75중량%와 1차 가공된(덖음 과정) 녹차 잎 25중량%를 사용하는 것을 제외하고는 상기 비교예 6-1과 동일한 방법을 사용하여 강자 가공된 인삼 다당체와 녹차 다당체 혼합물 70g을 얻었다.
Ginseng polysaccharide and 70 g of a green tea polysaccharide mixture were obtained by using the same method as in Comparative Example 6-1, except that 75% by weight of ginseng and 25% by weight of primary processed (roasted) green tea leaves were used.

[비교예 7-1] 인삼 밀자(蜜炙) 다당체의 제조[Comparative Example 7-1] Production of ginseng millet (polysaccharide)

건조한 인삼에 인삼 중량 대비 20~30%의 꿀을 인삼에 충분히 흡수시킨 다음 100~160℃에서 10분~1시간 동안 볶은 후 그늘에 말렸다. 이렇게 얻은 인삼 1kg에 정제수 10ℓ를 넣고, 3회 환류 추출한 다음, 15℃에서 1일간 침적시켰다. 그 후, 여과포 여과와 원심분리를 통해 잔사와 여액을 정제수 양의 1/10까지 감압 농축하였다. 여기에 농축된 양의 4배수의 에탄올을 적가하고 열풍 건조시켜 밀자 가공된 인삼 다당체 75g을 얻었다.
The dried ginseng was thoroughly absorbed in ginseng at 20 ~ 30% of the weight of ginseng, roasted at 100 ~ 160 ℃ for 10 minutes ~ 1 hour and dried in the shade. To 1 kg of the thus-obtained ginseng was added 10 L of purified water, refluxed three times, and then immersed at 15 캜 for 1 day. Thereafter, the filtrate and the filtrate were concentrated under reduced pressure to 1/10 of the amount of purified water through filtration of the filter cloth and centrifugation. To this was added a concentrated amount of ethanol in an amount of four times, followed by hot air drying to obtain 75 g of a milled ginseng polysaccharide.

[비교예 7-2] 녹차 밀자 다당체의 제조[Comparative Example 7-2] Production of green tea pollen polysaccharide

1차 가공된(덖음 과정) 녹차 잎을 사용하는 것을 제외하고는 상기 비교예 7-1과 동일한 방법을 사용하여 밀자 가공된 녹차 다당체 76g을 얻었다.
76 g of milled green tea polysaccharide was obtained in the same manner as in Comparative Example 7-1, except that the green tea leaves were used in the first processed (roughening process).

[실시예 7-1] 인삼 및 녹차 밀자 다당체의 제조(1:3의 중량비로 혼합)[Example 7-1] Production of ginseng and tea green tea polysaccharide (mixed at a weight ratio of 1: 3)

건조한 인삼 25중량%와 1차 가공된(덖음 과정) 녹차 잎 75중량%를 사용하는 것을 제외하고는 상기 비교예 7-1과 동일한 방법을 사용하여 밀자 가공된 인삼 다당체와 녹차 다당체 혼합물 74g을 얻었다.
Using the same method as in Comparative Example 7-1, 74 g of a mixture of ginseng polysaccharide and green tea polysaccharide was obtained, except that 25% by weight of dry ginseng and 75% by weight of first-processed (smoothing process) green tea leaves were used .

[실시예 7-2] 인삼 및 녹차 밀자 다당체의 제조(1:1의 중량비로 혼합)[Example 7-2] Preparation of ginseng and tea green tea polysaccharide (mixed at a weight ratio of 1: 1)

건조한 인삼 50중량%와 1차 가공된(덖음 과정) 녹차 잎 50중량%를 사용하는 것을 제외하고는 상기 비교예 7-1과 동일한 방법을 사용하여 밀자 가공된 인삼 다당체와 녹차 다당체 혼합물 75g을 얻었다.
75 g of a mixture of milled ginseng polysaccharide and green tea polysaccharide was obtained in the same manner as in Comparative Example 7-1, except that 50% by weight of dried ginseng and 50% by weight of primary processed green tea leaves were used .

[실시예 7-3] 인삼 및 녹차 밀자 다당체의 제조(3:1의 중량비로 혼합)[Example 7-3] Preparation of ginseng and green tea pollen polysaccharide (mixed at a weight ratio of 3: 1)

건조한 인삼 75중량%와 1차 가공된(덖음 과정) 녹차 잎 25중량%를 사용하는 것을 제외하고는 상기 비교예 7-1과 동일한 방법을 사용하여 밀자 가공된 인삼 다당체와 녹차 다당체 혼합물 74g을 얻었다.
Using the same method as in Comparative Example 7-1, 74 g of a mixture of ginseng polysaccharide and green tea polysaccharide was obtained, except that 75% by weight of dry ginseng and 25% by weight of primary processed (roasted) green tea leaves were used .

[비교예 8-1] 인삼 생품에서 추출한 다당체의 제조[Comparative Example 8-1] Preparation of polysaccharide extracted from ginseng product

건조된 인삼 1kg을 믹서기로 분쇄한 뒤 여기에 정제수 10ℓ를 넣고, 38~42℃ 온수로 24시간 교반 추출한 다음, 15℃에서 1일간 침적시켰다. 그 후, 여과포 여과와 원심분리를 통해 잔사와 여액을 분리하고, 분리된 여액을 정제수 양의 1/10까지 감압 농축하였다. 여기에 농축된 양의 4배수의 에탄올을 적가하고 열풍 건조시켜 인삼 다당체 65g을 얻었다.
1 kg of dried ginseng was pulverized with a blender, 10 L of purified water was added thereto, and the mixture was stirred for 24 hours at 38 to 42 ° C in hot water, and then immersed at 15 ° C for 1 day. Thereafter, the residue and the filtrate were separated by filtration and centrifugation through a filter cloth, and the separated filtrate was concentrated under reduced pressure to 1/10 of the amount of purified water. Four times as much ethanol as the concentrated amount was added dropwise, and the mixture was hot-air dried to obtain 65 g of ginseng polysaccharide.

[비교예 8-2] 녹차 생품에서 추출한 다당체의 제조[Comparative Example 8-2] Preparation of polysaccharide extracted from green tea product

1차 가공된(덖음 과정) 녹차 잎 1kg을 사용하는 것을 제외하고는 상기 비교예 8-1과 동일한 방법을 사용하여 녹차 다당체 72g을 얻었다.
72 g of green tea polysaccharide was obtained in the same manner as in Comparative Example 8-1, except that 1 kg of the first-processed green tea leaves were used.

[비교예 8-3] 인삼 및 녹차 생품에서 추출한 다당체 혼합물의 제조(1:3의 중량비로 혼합)[Comparative Example 8-3] Preparation of a polysaccharide mixture extracted from ginseng and green tea products (mixed at a weight ratio of 1: 3)

상기 비교예 8-1과 비교예 8-2에서 제조한 인삼 다당체와 녹차 다당체를 1:3의 비율로 단순 혼합하여 인삼 다당체와 녹차 다당체의 혼합물을 제조하였다.
The ginseng polysaccharide prepared in Comparative Example 8-1 and the green tea polysaccharide prepared in Comparative Example 8-2 were simply mixed at a ratio of 1: 3 to prepare a mixture of ginseng polysaccharide and green tea polysaccharide.

[비교예 8-4] 인삼 및 녹차 생품에서 추출한 다당체 혼합물의 제조(1:1의 중량비로 혼합)[Comparative Example 8-4] Preparation of a polysaccharide mixture extracted from ginseng and green tea products (mixed at a weight ratio of 1: 1)

상기 비교예 8-1과 비교예 8-2에서 제조한 인삼 다당체와 녹차 다당체를 1:1의 비율로 단순 혼합하여 인삼 다당체와 녹차 다당체의 혼합물을 제조하였다.
The mixture of ginseng polysaccharide and green tea polysaccharide prepared in Comparative Example 8-1 and Comparative Example 8-2 was simply mixed at a ratio of 1: 1 to prepare a mixture of ginseng polysaccharide and green tea polysaccharide.

[비교예 8-5] 인삼 및 녹차 생품에서 추출한 다당체 혼합물의 제조(3:1의 중량비로 혼합)[Comparative Example 8-5] Preparation of a polysaccharide mixture extracted from ginseng and green tea products (mixed at a weight ratio of 3: 1)

상기 비교예 8-1과 비교예 8-2에서 제조한 인삼 다당체와 녹차 다당체를 3:1의 비율로 단순 혼합하여 인삼 다당체와 녹차 다당체의 혼합물을 제조하였다.
The mixture of ginseng polysaccharide and green tea polysaccharide prepared in Comparative Example 8-1 and Comparative Example 8-2 was simply mixed at a ratio of 3: 1 to prepare a mixture of ginseng polysaccharide and green tea polysaccharide.

[참조예] 제형예 및 비교제형예의 제조Reference Example Preparation of Formulation Example and Comparative Formulation Example

하기 표 1 내지 5의 조성으로 샴푸를 제조하였다. 구체적으로, 계면활성제와 에틸렌글리콜디스테아레이트를 정제수에 첨가하여 80℃가 될 때까지 가열하여 균일하게 용해시킨 후, 교반하에 40℃까지 서서히 냉각시키고, 상기 혼합물에 본 발명에 따른 유효성분과 방부제, 점도조절제, 향료, 모발 컨디셔닝제를 투입하여 혼합한 후, 교반하에 실온까지 냉각시켜 제조하였다. Shampoos were prepared with the compositions of Tables 1 to 5 below. Specifically, the surfactant and ethylene glycol distearate were added to purified water, heated to 80 DEG C to dissolve uniformly, and then gradually cooled to 40 DEG C with stirring. To the mixture were added the active ingredient according to the present invention and a preservative, A viscosity adjusting agent, a perfume, and a hair conditioning agent were mixed and stirred, followed by cooling to room temperature.

성분ingredient 제형예Formulation Example 1-11-1 1-21-2 1-31-3 2-12-1 2-22-2 2-32-3 3-13-1 3-23-2 3-33-3 라우릴황산암모늄Ammonium lauryl sulfate 1010 1010 1010 1010 1010 1010 1010 1010 1010 폴리옥시에틸렌라우릴황산암모늄Polyoxyethylene lauryl ammonium sulfate 55 55 55 55 55 55 55 55 55 코코아미도프로필베타인Cocoamidopropyl betaine 22 22 22 22 22 22 22 22 22 에틸렌글리콜디스테아레이트Ethylene glycol distearate 1.51.5 1.51.5 1.51.5 1.51.5 1.51.5 1.51.5 1.51.5 1.51.5 1.51.5 코코일 모노에탄올아미드Cocoyl monoethanolamide 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 실시예 1-1Example 1-1 5.05.0 -- -- -- -- -- -- -- -- 실시예 1-2Examples 1-2 -- 5.05.0 -- -- -- -- -- -- -- 실시예 1-3Example 1-3 -- -- 5.05.0 -- -- -- -- -- -- 실시예 2-1Example 2-1 -- -- -- 5.05.0 -- -- -- -- -- 실시예 2-2Example 2-2 -- -- -- -- 5.05.0 -- -- -- -- 실시예 2-3Example 2-3 -- -- -- -- -- 5.05.0 -- -- -- 실시예 3-1Example 3-1 -- -- -- -- -- -- 5.05.0 -- -- 실시예 3-2Example 3-2 -- -- -- -- -- -- -- 5.05.0 -- 실시예 3-3Example 3-3 -- -- -- -- -- -- -- -- 5.05.0 폴리쿼터늄-10Polyquaternium-10 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 청색1호Blue No. 1 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 황색4호Yellow No. 4 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 메틸파라벤Methyl paraben 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 향료Spices 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 구연산Citric acid 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 디메치콘Dimethicone 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 water to 100to 100 to 100to 100 to 100to 100 to 100to 100 to 100to 100 to 100to 100 to 100to 100 to 100to 100 to 100to 100

성분ingredient 제형예Formulation Example 4-14-1 4-24-2 4-34-3 5-15-1 5-25-2 5-35-3 6-16-1 6-26-2 6-36-3 라우릴황산암모늄Ammonium lauryl sulfate 1010 1010 1010 1010 1010 1010 1010 1010 1010 폴리옥시에틸렌라우릴황산암모늄Polyoxyethylene lauryl ammonium sulfate 55 55 55 55 55 55 55 55 55 코코아미도프로필베타인Cocoamidopropyl betaine 22 22 22 22 22 22 22 22 22 에틸렌글리콜디스테아레이트Ethylene glycol distearate 1.51.5 1.51.5 1.51.5 1.51.5 1.51.5 1.51.5 1.51.5 1.51.5 1.51.5 코코일 모노에탄올아미드Cocoyl monoethanolamide 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 실시예 4-1Example 4-1 5.05.0 -- -- -- -- -- -- -- -- 실시예 4-2Example 4-2 -- 5.05.0 -- -- -- -- -- -- -- 실시예 4-3Example 4-3 -- -- 5.05.0 -- -- -- -- -- -- 실시예 5-1Example 5-1 -- -- -- 5.05.0 -- -- -- -- -- 실시예 5-2Example 5-2 -- -- -- -- 5.05.0 -- -- -- -- 실시예 5-3Example 5-3 -- -- -- -- -- 5.05.0 -- -- -- 실시예 6-1Example 6-1 -- -- -- -- -- -- 5.05.0 -- -- 실시예 6-2Example 6-2 -- -- -- -- -- -- -- 5.05.0 -- 실시예 6-3Example 6-3 -- -- -- -- -- -- -- -- 5.05.0 폴리쿼터늄-10Polyquaternium-10 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 청색1호Blue No. 1 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 황색4호Yellow No. 4 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 메틸파라벤Methyl paraben 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 향료Spices 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 구연산Citric acid 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 디메치콘Dimethicone 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 water to 100to 100 to 100to 100 to 100to 100 to 100to 100 to 100to 100 to 100to 100 to 100to 100 to 100to 100 to 100to 100

성분ingredient 제형예Formulation Example 비교제형예Comparative Formulation Example 7-17-1 7-27-2 7-37-3 1-11-1 1-21-2 2-12-1 2-22-2 3-13-1 3-23-2 라우릴황산암모늄Ammonium lauryl sulfate 1010 1010 1010 1010 1010 1010 1010 1010 1010 폴리옥시에틸렌라우릴황산암모늄Polyoxyethylene lauryl ammonium sulfate 55 55 55 55 55 55 55 55 55 코코아미도프로필베타인Cocoamidopropyl betaine 22 22 22 22 22 22 22 22 22 에틸렌글리콜디스테아레이트Ethylene glycol distearate 1.51.5 1.51.5 1.51.5 1.51.5 1.51.5 1.51.5 1.51.5 1.51.5 1.51.5 코코일 모노에탄올아미드Cocoyl monoethanolamide 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 실시예 7-1Example 7-1 5.05.0 -- -- -- -- -- -- -- -- 실시예 7-2Example 7-2 -- 5.05.0 -- -- -- -- -- -- -- 실시예 7-3Example 7-3 -- -- 5.05.0 -- -- -- -- -- -- 비교예 1-1Comparative Example 1-1 -- -- -- 5.05.0 -- -- -- -- -- 비교예 1-2Comparative Example 1-2 -- -- -- -- 5.05.0 -- -- -- -- 비교예 2-1Comparative Example 2-1 -- -- -- -- -- 5.05.0 -- -- -- 비교예 2-2Comparative Example 2-2 -- -- -- -- -- -- 5.05.0 -- -- 비교예 3-1Comparative Example 3-1 -- -- -- -- -- -- -- 5.05.0 -- 비교예 3-2Comparative Example 3-2 -- -- -- -- -- -- -- -- 5.05.0 폴리쿼터늄-10Polyquaternium-10 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 청색1호Blue No. 1 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 황색4호Yellow No. 4 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 메틸파라벤Methyl paraben 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 향료Spices 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 구연산Citric acid 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 디메치콘Dimethicone 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 water to 100to 100 to 100to 100 to 100to 100 to 100to 100 to 100to 100 to 100to 100 to 100to 100 to 100to 100 to 100to 100

성분ingredient 비교제형예Comparative Formulation Example 4-14-1 4-24-2 5-15-1 5-25-2 6-16-1 6-26-2 7-17-1 7-27-2 라우릴황산암모늄Ammonium lauryl sulfate 1010 1010 1010 1010 1010 1010 1010 1010 폴리옥시에틸렌라우릴황산암모늄Polyoxyethylene lauryl ammonium sulfate 55 55 55 55 55 55 55 55 코코아미도프로필베타인Cocoamidopropyl betaine 22 22 22 22 22 22 22 22 에틸렌글리콜디스테아레이트Ethylene glycol distearate 1.51.5 1.51.5 1.51.5 1.51.5 1.51.5 1.51.5 1.51.5 1.51.5 코코일 모노에탄올아미드Cocoyl monoethanolamide 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 비교예 4-1Comparative Example 4-1 5.05.0 -- -- -- -- -- -- -- 비교예 4-2Comparative Example 4-2 -- 5.05.0 -- -- -- -- -- -- 비교예 5-1Comparative Example 5-1 -- -- 5.05.0 -- -- -- -- -- 비교예 5-2Comparative Example 5-2 -- -- -- 5.05.0 -- -- -- -- 비교예 6-1Comparative Example 6-1 -- -- -- -- 5.05.0 -- -- -- 비교예 6-2Comparative Example 6-2 -- -- -- -- -- 5.05.0 -- -- 비교예 7-1Comparative Example 7-1 -- -- -- -- -- -- 5.05.0 -- 비교예 7-2Comparative Example 7-2 -- -- -- -- -- -- -- 5.05.0 폴리쿼터늄-10Polyquaternium-10 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 청색1호Blue No. 1 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 황색4호Yellow No. 4 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 메틸파라벤Methyl paraben 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 향료Spices 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 구연산Citric acid 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 디메치콘Dimethicone 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 water to 100to 100 to 100to 100 to 100to 100 to 100to 100 to 100to 100 to 100to 100 to 100to 100 to 100to 100

성분ingredient 비교제형예Comparative Formulation Example 8-18-1 8-28-2 8-38-3 8-48-4 8-58-5 99 라우릴황산암모늄Ammonium lauryl sulfate 1010 1010 1010 1010 1010 1010 폴리옥시에틸렌라우릴황산암모늄Polyoxyethylene lauryl ammonium sulfate 55 55 55 55 55 55 코코아미도프로필베타인Cocoamidopropyl betaine 22 22 22 22 22 22 에틸렌글리콜디스테아레이트Ethylene glycol distearate 1.51.5 1.51.5 1.51.5 1.51.5 1.51.5 1.51.5 코코일 모노에탄올아미드Cocoyl monoethanolamide 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 비교예 8-1Comparative Example 8-1 5.05.0 -- -- -- -- -- 비교예 8-2Comparative Example 8-2 -- 5.05.0 -- -- -- -- 비교예 8-3Comparative Example 8-3 -- -- 5.05.0 -- -- -- 비교예 8-4Comparative Example 8-4 -- -- -- 5.05.0 -- -- 비교예 8-5Comparative Example 8-5 -- -- -- -- 5.05.0 -- 폴리쿼터늄-10Polyquaternium-10 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 청색1호Blue No. 1 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 0.00020.0002 황색4호Yellow No. 4 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 0.00010.0001 메틸파라벤Methyl paraben 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 향료Spices 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 0.80.8 구연산Citric acid 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 0.10.1 디메치콘Dimethicone 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 water to 100to 100 to 100to 100 to 100to 100 to 100to 100 to 100to 100 to 100to 100

[시험예 1] 비듬감소 효과 시험[Test Example 1] Dandruff reduction effect test

비듬이 비교적 많은 19~35세의 남성 410명을 선정하여 10명씩 41개 그룹으로 나누고 각 그룹에 대하여 제형예 1-1 내지 7-3, 비교제형예 1-1 내지 9의 샴푸를 1개월간 다음과 같은 방식으로 사용하게 한 후 비듬 감소율을 측정하였다.410 males aged 19 to 35 years with a relatively large number of dandruff were selected and divided into 41 groups of 10, and the shampoos of Formulation Examples 1-1 to 7-3 and Comparative Formulation Examples 1-1 to 9 were applied for 1 month And the dysrhythmia reduction rate was measured.

시험 개시전에 보통 통상의 샴푸로 세발하고, 세발 후 2일간 누적된 비듬을 채집하여 채집된 비듬의 중량과 각각의 샴푸로 2일에 한번씩 세발하여 시험종료 후 2일간 누적된 비듬의 중량을 비교 평가하였다. 이때 누적된 비듬은 진공 흡입 장치로써 두피로부터 직접 채집하여, 하기 수학식 1에 의거하여 비듬 감소율을 구하고 그 결과를 하기 표 6에 나타내었다. Before the start of the test, the dandruff was sampled with normal shampoo, and the dandruff accumulated for 2 days after shaking was collected. The dandruff weight was sampled every two days with each shampoo. Respectively. The accumulated dandruff was collected directly from the scalp using a vacuum suction device, and the dandruff reduction rate was calculated according to the following equation (1). The results are shown in Table 6 below.

Figure pat00001
Figure pat00001

비듬감소율(%)Dandruff reduction rate (%) 제형예 1-1Formulation Example 1-1 53.553.5 제형예 1-2Formulation Example 1-2 52.452.4 제형예 1-3Formulation Example 1-3 52.352.3 비교제형예 1-1Comparative Formulation Example 1-1 36.936.9 비교제형예 1-2Comparative Formulation Example 1-2 36.636.6 제형예 2-1Formulation Example 2-1 49.749.7 제형예 2-2Formulation Example 2-2 49.449.4 제형예 2-3Formulation Example 2-3 49.549.5 비교제형예 2-1Comparative Formulation Example 2-1 34.834.8 비교제형예 2-2Comparative Formulation Example 2-2 33.933.9 제형예 3-1Formulation Example 3-1 47.547.5 제형예 3-2Formulation Example 3-2 46.046.0 제형예 3-3Formulation Example 3-3 45.845.8 비교제형예 3-1Comparative Formulation Example 3-1 35.435.4 비교제형예 3-2Comparative Formulation Example 3-2 34.034.0 제형예 4-1Formulation Example 4-1 51.951.9 제형예 4-2Formulation Example 4-2 50.750.7 제형예 4-3Formulation Example 4-3 50.150.1 비교제형예 4-1Comparative Formulation Example 4-1 36.236.2 비교제형예 4-2Comparative Formulation Example 4-2 35.435.4 제형예 5-1Formulation Example 5-1 50.750.7 제형예 5-2Formulation Example 5-2 49.949.9 제형예 5-3Formulation Example 5-3 48.248.2 비교제형예 5-1Comparative Formulation Example 5-1 35.735.7 비교제형예 5-2Comparative Formulation Example 5-2 34.234.2 제형예 6-1Formulation Example 6-1 49.349.3 제형예 6-2Formulation Example 6-2 48.848.8 제형예 6-3Formulation Example 6-3 49.049.0 비교제형예 6-1Comparative Formulation Example 6-1 35.235.2 비교제형예 6-2Comparative Formulation Example 6-2 34.934.9 제형예 7-1Formulation Example 7-1 50.250.2 제형예 7-2Formulation Example 7-2 50.450.4 제형예 7-3Formulation Example 7-3 48.948.9 비교제형예 7-1Comparative Formulation Example 7-1 35.435.4 비교제형예 7-2Comparative Formulation Example 7-2 35.835.8 비교제형예 8-1Comparative Formulation Example 8-1 30.830.8 비교제형예 8-2Comparative Formulation Example 8-2 29.829.8 비교제형예 8-3Comparative Formulation Example 8-3 34.534.5 비교제형예 8-4Comparative Formulation Examples 8-4 34.234.2 비교제형예 8-5Comparative Formulation Examples 8-5 32.932.9 비교제형예 9Comparative Formulation Example 9 2.32.3

상기한 표 6에서 알 수 있는 바와 같이, 일반적인 인삼 및 녹차 다당체를 사용하는 경우보다 포제 처리한 인삼 또는 녹차로부터 추출한 다당체를 사용하는 경우가 비듬방지 효과가 더 우수함을 알 수 있다. 또한 인삼 다당체와 녹차 다당체를 각각 사용하는 경우보다 이를 혼합하여 사용할 경우에 효능이 보다 증가하였으며, 특히 포제 처리한 인삼 및 녹차로부터 추출한 다당체를 혼합하여 사용하는 경우 매우 우수한 비듬방지 효과를 나타냄을 알 수 있다.
As can be seen from the above Table 6, it can be seen that the use of polysaccharide extracted from green ginseng or green tea, which is more fungicide treated than general ginseng and green tea polysaccharide, is more effective in preventing dandruff. In addition, the effectiveness of the mixture of ginseng polysaccharide and green tea polysaccharide was higher than that of the case of using ginseng polysaccharide and green tea polysaccharide, respectively. Especially, when the polysaccharides extracted from forage-treated ginseng and green tea were mixed, have.

[시험예 2] 두피 가려움증 방지 효과 시험[Test Example 2] Test for preventing the itchiness of the scalp

비교적 두피 가려움을 심하게 느끼는 25~45세의 남녀 410명을 선정하여 10명씩 41개 그룹으로 나누고 각 그룹에 대하여 제형예 1-1 내지 7-3, 비교제형예 1-1 내지 9의 샴푸를 3일에 1회씩 2주간 사용하게 한 후 두피 가려움 방지 효과를 하기 평가기준을 통하여 평가하고 그 결과를 하기 표 7에 나타내었다.
Forty-one men and women aged 25 to 45 years with severe scalp itching were selected and divided into 41 groups of 10, and the shampoos of Formulation Examples 1-1 to 7-3 and Comparative Formulation Examples 1-1 to 9 were divided into groups of 3 The results are shown in Table 7 below. ≪ tb >< TABLE >

[평가 기준][Evaluation standard]

매우 우수하다 - 5점Very good - 5 points

우수하다 - 4점Excellent - 4 points

보통이다 - 3점Usually - 3 points

불량하다 - 2점Poor - 2 points

매우 불량하다 - 1점Very bad - 1 point

점수score 제형예 1-1Formulation Example 1-1 4.14.1 제형예 1-2Formulation Example 1-2 4.04.0 제형예 1-3Formulation Example 1-3 4.04.0 비교제형예 1-1Comparative Formulation Example 1-1 3.73.7 비교제형예 1-2Comparative Formulation Example 1-2 3.73.7 제형예 2-1Formulation Example 2-1 3.83.8 제형예 2-2Formulation Example 2-2 3.73.7 제형예 2-3Formulation Example 2-3 3.63.6 비교제형예 2-1Comparative Formulation Example 2-1 3.43.4 비교제형예 2-2Comparative Formulation Example 2-2 3.33.3 제형예 3-1Formulation Example 3-1 3.83.8 제형예 3-2Formulation Example 3-2 3.83.8 제형예 3-3Formulation Example 3-3 3.73.7 비교제형예 3-1Comparative Formulation Example 3-1 3.43.4 비교제형예 3-2Comparative Formulation Example 3-2 3.43.4 제형예 4-1Formulation Example 4-1 4.04.0 제형예 4-2Formulation Example 4-2 3.93.9 제형예 4-3Formulation Example 4-3 4.04.0 비교제형예 4-1Comparative Formulation Example 4-1 3.63.6 비교제형예 4-2Comparative Formulation Example 4-2 3.53.5 제형예 5-1Formulation Example 5-1 4.04.0 제형예 5-2Formulation Example 5-2 3.93.9 제형예 5-3Formulation Example 5-3 3.73.7 비교제형예 5-1Comparative Formulation Example 5-1 3.53.5 비교제형예 5-2Comparative Formulation Example 5-2 3.33.3 제형예 6-1Formulation Example 6-1 3.83.8 제형예 6-2Formulation Example 6-2 3.63.6 제형예 6-3Formulation Example 6-3 3.53.5 비교제형예 6-1Comparative Formulation Example 6-1 3.33.3 비교제형예 6-2Comparative Formulation Example 6-2 3.23.2 제형예 7-1Formulation Example 7-1 4.04.0 제형예 7-2Formulation Example 7-2 3.83.8 제형예 7-3Formulation Example 7-3 3.73.7 비교제형예 7-1Comparative Formulation Example 7-1 3.43.4 비교제형예 7-2Comparative Formulation Example 7-2 3.43.4 비교제형예 8-1Comparative Formulation Example 8-1 3.13.1 비교제형예 8-2Comparative Formulation Example 8-2 2.92.9 비교제형예 8-3Comparative Formulation Example 8-3 3.43.4 비교제형예 8-4Comparative Formulation Examples 8-4 3.53.5 비교제형예 8-5Comparative Formulation Examples 8-5 3.43.4 비교제형예 9Comparative Formulation Example 9 2.12.1

상기한 표 7에서 알 수 있는 바와 같이, 일반적인 인삼 또는 녹차 다당체를 사용하는 경우보다 포제 처리한 인삼 또는 녹차로부터 추출한 다당체를 사용하는 경우가 두피 가려움증의 방지 효과가 더 우수함을 알 수 있다. 또한 인삼 다당체와 녹차 다당체를 각각 사용하는 경우보다 이를 혼합하여 사용할 경우에 효능이 증가하였으며, 특히 포제 처리한 인삼 및 녹차로부터 추출한 다당체를 혼합하여 사용하는 경우 두피 가려움증을 보다 효과적으로 방지할 수 있음을 알 수 있다.
As can be seen from the above Table 7, it can be seen that the use of polysaccharide extracted from ginseng or green tea treated with forage treatment is superior to the effect of using common ginseng or green tea polysaccharide to prevent scalp itching. In addition, it was found that the effect of the mixture of ginseng polysaccharide and green tea polysaccharide was increased when they were mixed with each other. Especially, when polysaccharide extracted from forged ginseng and green tea were mixed, it is possible to prevent scalp itching more effectively .

[시험예 3] 모낭 모유두 세포 증식 효과[Test Example 3] Effect of proliferation of dermal papilla cells

모발을 구성하는 케라틴 단백질은 모근부 케라틴형성세포 (keratinocyte)에서 생성되며, 이 케라틴형성세포는 모유두 세포로부터 분화된다. 본 조성물의 모유두 세포를 활성을 평가하기 위하여 본 발명에서는 DP6(rat immortalized dermal papilla cell) 세포주를 사용하였다(Wendy Filsell, Journal of Cell Science 107, 1761-1772 (1994)). 본 모유두 세포주는 수컷 PVG 래트 수염의 모근에서 현미해부(microdissection) 방법으로 분리하여 배양한 세포주이며, 10% FBS(Fetal bovine serum)가 함유된 DMEM(Dulbecco's modified Eagle's medium, Gibco BRL, Gaithersburg, MD, USA)에서 5% CO2, 37℃가 유지되는 인큐베이터에서 24시간 동안 배양하였다. DP6을 96 웰 플레이트에 넣고 24시간 동안 37℃ 인큐베이터에서 배양한 후 상기 실시예 1-1 내지 7-3 및 비교예 1-1 내지 8-5의 다당체 또는 다당체 혼합물을 각각 5ppm, 10ppm 및 20ppm의 농도로 처리하였다. 약물 처리 24시간 경과 후에 WST-1 키트(Roche)를 사용하여 세포 증식능을 측정하였다. 결과는 하기 표 8에 나타내었다.The keratin proteins that make up the hair are produced in hair follicle keratinocyte, which keratinocytes differentiate from the dermal papilla cells. In order to evaluate the activity of the dermal papilla cells of the present composition, DP6 (rat immortalized dermal papilla cell) cell line was used in the present invention (Wendy Filsell, Journal of Cell Science 107, 1761-1772 (1994)). This dermal papilla cell line was obtained by microdissection in the hair roots of male PVG rat beard and cultured in DMEM (Dulbecco's modified Eagle's medium, Gibco BRL, Gaithersburg, MD, USA) containing 10% FBS (Fetal bovine serum) USA) for 24 hours in an incubator maintained at 37 ° C in 5% CO 2 . DP6 was added to a 96-well plate and cultured in a 37 ° C incubator for 24 hours. Then, the polysaccharide or polysaccharide mixture of each of Examples 1-1 to 7-3 and Comparative Examples 1-1 to 8-5 was treated with 5 ppm, 10 ppm and 20 ppm Lt; / RTI > After 24 hours of drug treatment, cell proliferation was measured using WST-1 kit (Roche). The results are shown in Table 8 below.

구분division 세포 증식능(%)Cell proliferation (%) 무처리 대조군(Control)Untreated Control (Control) 100100 실시예 1-1Example 1-1 5ppm5 ppm 114114 10ppm10ppm 119119 20ppm20 ppm 133133 실시예 1-2Examples 1-2 5ppm5 ppm 115115 10ppm10ppm 121121 20ppm20 ppm 134134 실시예 1-3Example 1-3 5ppm5 ppm 114114 10ppm10ppm 120120 20ppm20 ppm 132132 비교예 1-1Comparative Example 1-1 5ppm5 ppm 112112 10ppm10ppm 116116 20ppm20 ppm 128128 비교예 1-2Comparative Example 1-2 5ppm5 ppm 113113 10ppm10ppm 119119 20ppm20 ppm 129129 실시예 2-1Example 2-1 5ppm5 ppm 114114 10ppm10ppm 120120 20ppm20 ppm 132132 실시예 2-2Example 2-2 5ppm5 ppm 114114 10ppm10ppm 121121 20ppm20 ppm 133133 실시예 2-3Example 2-3 5ppm5 ppm 115115 10ppm10ppm 122122 20ppm20 ppm 134134 비교예 2-1Comparative Example 2-1 5ppm5 ppm 113113 10ppm10ppm 117117 20ppm20 ppm 128128 비교예 2-2Comparative Example 2-2 5ppm5 ppm 112112 10ppm10ppm 117117 20ppm20 ppm 129129 실시예 3-1Example 3-1 5ppm5 ppm 115115 10ppm10ppm 121121 20ppm20 ppm 135135 실시예 3-2Example 3-2 5ppm5 ppm 115115 10ppm10ppm 122122 20ppm20 ppm 136136 실시예 3-3Example 3-3 5ppm5 ppm 114114 10ppm10ppm 120120 20ppm20 ppm 133133 비교예 3-1Comparative Example 3-1 5ppm5 ppm 113113 10ppm10ppm 118118 20ppm20 ppm 130130 비교예 3-2Comparative Example 3-2 5ppm5 ppm 113113 10ppm10ppm 119119 20ppm20 ppm 130130 실시예 4-1Example 4-1 5ppm5 ppm 114114 10ppm10ppm 119119 20ppm20 ppm 132132 실시예 4-2Example 4-2 5ppm5 ppm 115115 10ppm10ppm 121121 20ppm20 ppm 134134 실시예 4-3Example 4-3 5ppm5 ppm 115115 10ppm10ppm 121121 20ppm20 ppm 133133 비교예 4-1Comparative Example 4-1 5ppm5 ppm 112112 10ppm10ppm 118118 20ppm20 ppm 128128 비교예 4-2Comparative Example 4-2 5ppm5 ppm 113113 10ppm10ppm 120120 20ppm20 ppm 131131 실시예 5-1Example 5-1 5ppm5 ppm 115115 10ppm10ppm 120120 20ppm20 ppm 135135 실시예 5-2Example 5-2 5ppm5 ppm 115115 10ppm10ppm 121121 20ppm20 ppm 135135 실시예 5-3Example 5-3 5ppm5 ppm 116116 10ppm10ppm 121121 20ppm20 ppm 136136 비교예 5-1Comparative Example 5-1 5ppm5 ppm 113113 10ppm10ppm 119119 20ppm20 ppm 130130 비교예 5-2Comparative Example 5-2 5ppm5 ppm 114114 10ppm10ppm 121121 20ppm20 ppm 132132 실시예 6-1Example 6-1 5ppm5 ppm 115115 10ppm10ppm 121121 20ppm20 ppm 134134 실시예 6-2Example 6-2 5ppm5 ppm 116116 10ppm10ppm 120120 20ppm20 ppm 134134 실시예 6-3Example 6-3 5ppm5 ppm 115115 10ppm10ppm 122122 20ppm20 ppm 133133 비교예 6-1Comparative Example 6-1 5ppm5 ppm 113113 10ppm10ppm 119119 20ppm20 ppm 132132 비교예 6-2Comparative Example 6-2 5ppm5 ppm 113113 10ppm10ppm 120120 20ppm20 ppm 132132 실시예 7-1Example 7-1 5ppm5 ppm 114114 10ppm10ppm 119119 20ppm20 ppm 133133 실시예 7-2Example 7-2 5ppm5 ppm 114114 10ppm10ppm 119119 20ppm20 ppm 132132 실시예 7-3Example 7-3 5ppm5 ppm 115115 10ppm10ppm 120120 20ppm20 ppm 133133 비교예 7-1Comparative Example 7-1 5ppm5 ppm 112112 10ppm10ppm 118118 20ppm20 ppm 128128 비교예 7-2Comparative Example 7-2 5ppm5 ppm 113113 10ppm10ppm 119119 20ppm20 ppm 129129 비교예 8-1Comparative Example 8-1 5ppm5 ppm 108108 10ppm10ppm 113113 20ppm20 ppm 121121 비교예 8-2Comparative Example 8-2 5ppm5 ppm 110110 10ppm10ppm 115115 20ppm20 ppm 125125 비교예 8-3Comparative Example 8-3 5ppm5 ppm 113113 10ppm10ppm 117117 20ppm20 ppm 129129 비교예 8-4Comparative Example 8-4 5ppm5 ppm 112112 10ppm10ppm 117117 20ppm20 ppm 127127 비교예 8-5Comparative Example 8-5 5ppm5 ppm 112112 10ppm10ppm 117117 20ppm20 ppm 126126

상기 표 8에서 알 수 있는 바와 같이, 일반적인 인삼 또는 녹차 다당체를 사용하는 경우보다 포제 처리한 인삼 또는 녹차로부터 추출한 다당체를 사용하는 경우가 모유두 세포의 증식 효과가 더 우수함을 알 수 있다. 또한 인삼 다당체와 녹차 다당체를 각각 사용하는 경우보다 이를 혼합하여 사용할 경우에 세포 증식능이 더 높았으며, 특히 포제 처리한 인삼 및 녹차로부터 추출한 다당체를 혼합하여 사용하는 경우 모유두 세포의 증식이 더 효과적으로 증가하는 한편, 이는 농도 의존적으로 유의차있게 증가하는 것을 확인할 수 있다.
As can be seen from the above Table 8, the use of polysaccharide extracted from green tea or green tea, which has been subjected to forage treatment, is superior to that of general ginseng or green tea polysaccharide. In addition, when the ginseng polysaccharide and the green tea polysaccharide were used in combination, the cell proliferative ability was higher in the case of using them, and in particular, when the polysaccharide extracted from the folate-treated ginseng and green tea was mixed, the proliferation of the papilla cells was more effectively increased On the other hand, it can be confirmed that this is significantly increased depending on the concentration.

[시험예 4] 칼륨 이온 채널 활성 증가 효과 평가[Test Example 4] Evaluation of Potassium Ion Channel Activity Increase Effect

탈모 치료제인 미녹시딜은 잠재적인 미토콘드리아 칼륨 이온 채널 오프너(KATP channel opener)로 알려져 있으며, 안드로겐성 탈모의 치료에 사용되는 대표적인 약물이다. 이러한 미녹시딜의 기작을 평가하기 위해서 두피의 진피를 구성하는 섬유아 세포에서 KATP 채널을 막는 톨부타미드(SIGMA AlDRICH, T0891)를 처리하여 세포증식을 억제하고, 다시 칼륨 이온 채널을 열어 세포증식이 회복되는 시험법을 사용하였다. Minoxidil, a remedy for depilation, is known as a potential mitochondrial potassium ion channel opener (K ATP channel opener) and is a representative drug used for the treatment of androgenetic alopecia. In order to evaluate the mechanism of minoxidil, cell proliferation was inhibited by treatment with tolbutamide (SIGMA AlDRICH, T0891) blocking K ATP channel in fibroblasts constituting the dermis of the scalp, The restored test method was used.

본 조성물의 KATP 채널 오프너로서의 기능을 평가하기 위해서 본 발명에서는 섬유아세포주인 NIH3T3(Mouse embryonic fibroblast cell line) 세포주를 사용하였다. 본 세포주는 NIH 스위스 마우스 배아(Swiss mouse embryo)에서 분리한 섬유아 세포주를 3T3 프로토콜로 자연 불멸화시킨 세포주이다. 상기 세포주는 10% FBS가 함유된 DMEM(Gibco BRL, Gaithersburg, MD, USA)에서 5% CO2, 37℃가 유지되는 인큐베이터에서 24시간 동안 배양하였다. NIH3T3을 96웰 플레이트에 넣고 24시간 동안 37℃ 인큐베이터에서 배양한 후 2.5mM 톨부타미드를 처리하고, 10분 뒤에 양성대조군인 미녹시딜 10μM과 상기 실시예 1-1 내지 7-3 및 비교예 1-1 내지 8-5의 다당체 또는 다당체 혼합물을 각각 2.5ppm, 5ppm 및 10ppm의 농도로 처리하며, 약물 처리 48시간 경과 후에 WST-1 키트(Roche)를 사용하여 세포 증식능을 측정하였다. 결과는 하기 표 9에 나타내었다.To evaluate the function of this composition as a K ATP channel opener, the fibroblast cell line NIH3T3 (mouse embryonic fibroblast cell line) cell line was used in the present invention. This cell line is a cell line obtained by natural immortalization of fibroblasts isolated from NIH Swiss mouse embryo by the 3T3 protocol. The cells were cultured in DMEM (Gibco BRL, Gaithersburg, MD, USA) containing 10% FBS for 24 hours in an incubator maintained at 37 ° C in 5% CO 2 . NIH3T3 was added to a 96-well plate, cultured in a 37 ° C incubator for 24 hours, treated with 2.5 mM tolbutamide, and 10 minutes later, 10 μM of minoxidil, a positive control, and the cells of Examples 1-1 to 7- 1 to 8-5 polysaccharide or polysaccharide mixtures were treated at concentrations of 2.5 ppm, 5 ppm and 10 ppm, respectively, and cell proliferative activity was measured using WST-1 kit (Roche) after 48 hours of drug treatment. The results are shown in Table 9 below.

구분division 세포 증식능(%)Cell proliferation (%) 무처리 대조군(Control)Untreated Control (Control) 100100 미녹시딜Minoxidil 132132 실시예 1-1Example 1-1 2.5ppm2.5 ppm 114114 5ppm5 ppm 118118 10ppm10ppm 127127 실시예 1-2Examples 1-2 2.5ppm2.5 ppm 113113 5ppm5 ppm 117117 10ppm10ppm 126126 실시예 1-3Example 1-3 2.5ppm2.5 ppm 113113 5ppm5 ppm 118118 10ppm10ppm 127127 비교예 1-1Comparative Example 1-1 2.5ppm2.5 ppm 110110 5ppm5 ppm 114114 10ppm10ppm 121121 비교예 1-2Comparative Example 1-2 2.5ppm2.5 ppm 109109 5ppm5 ppm 113113 10ppm10ppm 120120 실시예 2-1Example 2-1 2.5ppm2.5 ppm 112112 5ppm5 ppm 117117 10ppm10ppm 126126 실시예 2-2Example 2-2 2.5ppm2.5 ppm 112112 5ppm5 ppm 116116 10ppm10ppm 125125 실시예 2-3Example 2-3 2.5ppm2.5 ppm 112112 5ppm5 ppm 117117 10ppm10ppm 126126 비교예 2-1Comparative Example 2-1 2.5ppm2.5 ppm 108108 5ppm5 ppm 113113 10ppm10ppm 120120 비교예 2-2Comparative Example 2-2 2.5ppm2.5 ppm 108108 5ppm5 ppm 114114 10ppm10ppm 120120 실시예 3-1Example 3-1 2.5ppm2.5 ppm 114114 5ppm5 ppm 118118 10ppm10ppm 127127 실시예 3-2Example 3-2 2.5ppm2.5 ppm 114114 5ppm5 ppm 117117 10ppm10ppm 127127 실시예 3-3Example 3-3 2.5ppm2.5 ppm 113113 5ppm5 ppm 117117 10ppm10ppm 126126 비교예 3-1Comparative Example 3-1 2.5ppm2.5 ppm 109109 5ppm5 ppm 113113 10ppm10ppm 120120 비교예 3-2Comparative Example 3-2 2.5ppm2.5 ppm 110110 5ppm5 ppm 114114 10ppm10ppm 121121 실시예 4-1Example 4-1 2.5ppm2.5 ppm 112112 5ppm5 ppm 117117 10ppm10ppm 126126 실시예 4-2Example 4-2 2.5ppm2.5 ppm 113113 5ppm5 ppm 117117 10ppm10ppm 127127 실시예 4-3Example 4-3 2.5ppm2.5 ppm 113113 5ppm5 ppm 118118 10ppm10ppm 128128 비교예 4-1Comparative Example 4-1 2.5ppm2.5 ppm 109109 5ppm5 ppm 113113 10ppm10ppm 121121 비교예 4-2Comparative Example 4-2 2.5ppm2.5 ppm 109109 5ppm5 ppm 115115 10ppm10ppm 122122 실시예 5-1Example 5-1 2.5ppm2.5 ppm 113113 5ppm5 ppm 117117 10ppm10ppm 125125 실시예 5-2Example 5-2 2.5ppm2.5 ppm 113113 5ppm5 ppm 116116 10ppm10ppm 126126 실시예 5-3Example 5-3 2.5ppm2.5 ppm 113113 5ppm5 ppm 117117 10ppm10ppm 126126 비교예 5-1Comparative Example 5-1 2.5ppm2.5 ppm 108108 5ppm5 ppm 112112 10ppm10ppm 120120 비교예 5-2Comparative Example 5-2 2.5ppm2.5 ppm 108108 5ppm5 ppm 113113 10ppm10ppm 120120 실시예 6-1Example 6-1 2.5ppm2.5 ppm 113113 5ppm5 ppm 118118 10ppm10ppm 127127 실시예 6-2Example 6-2 2.5ppm2.5 ppm 114114 5ppm5 ppm 118118 10ppm10ppm 127127 실시예 6-3Example 6-3 2.5ppm2.5 ppm 114114 5ppm5 ppm 119119 10ppm10ppm 128128 비교예 6-1Comparative Example 6-1 2.5ppm2.5 ppm 109109 5ppm5 ppm 114114 10ppm10ppm 119119 비교예 6-2Comparative Example 6-2 2.5ppm2.5 ppm 110110 5ppm5 ppm 115115 10ppm10ppm 120120 실시예 7-1Example 7-1 2.5ppm2.5 ppm 113113 5ppm5 ppm 117117 10ppm10ppm 126126 실시예 7-2Example 7-2 2.5ppm2.5 ppm 113113 5ppm5 ppm 117117 10ppm10ppm 127127 실시예 7-3Example 7-3 2.5ppm2.5 ppm 114114 5ppm5 ppm 118118 10ppm10ppm 128128 비교예 7-1Comparative Example 7-1 2.5ppm2.5 ppm 108108 5ppm5 ppm 113113 10ppm10ppm 119119 비교예 7-2Comparative Example 7-2 2.5ppm2.5 ppm 110110 5ppm5 ppm 114114 10ppm10ppm 121121 비교예 8-1Comparative Example 8-1 2.5ppm2.5 ppm 110110 5ppm5 ppm 114114 10ppm10ppm 122122 비교예 8-2Comparative Example 8-2 2.5ppm2.5 ppm 111111 5ppm5 ppm 115115 10ppm10ppm 124124 비교예 8-3Comparative Example 8-3 2.5ppm2.5 ppm 111111 5ppm5 ppm 116116 10ppm10ppm 125125 비교예 8-4Comparative Example 8-4 2.5ppm2.5 ppm 106106 5ppm5 ppm 111111 10ppm10ppm 119119 비교예 8-5Comparative Example 8-5 2.5ppm2.5 ppm 106106 5ppm5 ppm 112112 10ppm10ppm 118118

상기 표 9에서 알 수 있는 바와 같이, 일반적인 인삼 또는 녹차 다당체를 사용하는 경우보다 포제 처리한 인삼 또는 녹차로부터 추출한 다당체를 사용하는 경우가 섬유아 세포의 증식 회복 효과가 더 우수함을 알 수 있다. 또한 인삼 다당체와 녹차 다당체를 각각 사용하는 경우보다 이를 혼합하여 사용할 경우에 세포 증식능이 더 높았으며, 특히 포제 처리한 인삼 및 녹차로부터 추출한 다당체를 혼합하여 사용하는 경우 섬유아 세포의 증식 회복이 더 효과적으로 증가하는 한편, 이는 농도 의존적으로 유의차있게 증가하는 것을 확인할 수 있다. 또한, 포제 처리한 인삼 및 녹차로부터 추출한 다당체를 혼합하여 처리한 경우에는 미녹시딜 수준으로 섬유아 세포의 증식이 회복되는 것을 확인할 수 있다.
As can be seen from the above Table 9, the effect of using the polysaccharide extracted from the ginseng or green tea treated with the forage treatment is better than that of the normal ginseng or green tea polysaccharide. In addition, the cell proliferative capacity was higher when the ginseng polysaccharide and the green tea polysaccharide were mixed, respectively. In particular, when the polysaccharide extracted from the ginseng powder and the green tea mixed with the fossilized ginseng was used, the proliferation restoration of the fibroblast cell was more effective , While it increases significantly in a concentration-dependent manner. In addition, it was confirmed that the proliferation of fibroblast cells was restored to the minoxidil level when mixed with the polysaccharide extracted from the green tea and the fodder-treated ginseng.

[시험예 5] 멜라닌 생성 촉진 효과 시험[Test Example 5] Test for promoting melanin production

RPMI 배지에 5%의 우태아혈청, 100IU의 페니시린G 및 0.2μM의 TPA를 첨가한 배지에 멜라닌 세포(melan-a)를 24웰플레이트(24-well microtiter plate)에 50,000세포/웰이 되도록 분주하였다. 다음날 분주된 세포에 시험 물질로서 실시예 1-1 내지 7-3 및 비교예 1-1 내지 8-5의 다당체 또는 다당체 혼합물을 최종농도 10ppm 또는 50ppm으로 처리하고, 음성 대조군으로는 0.1% DMSO를, 양성 대조군으로는 100μM IBMX를 처리한 후에 37℃ 온도에서 3일간 배양하였다. 배양 후, PBS로 웰을 씻어주고 1N NaOH를 100㎕씩 넣은 후 세포 안의 멜라닌을 용해시켰다. 용해된 멜라닌의 흡광도를 평판배양측정기(microplate reader)를 이용하여 405nm에서 측정하였다. 인삼 및 녹차 다당체의 멜라닌 생성 촉진 효과를 대조군과 비교한 결과는 하기 표 10에 나타내었다.Melanocytes (melan-a) were added to a 24-well microtiter plate at 50,000 cells / well in RPMI medium supplemented with 5% fetal bovine serum, 100 IU penicillin G and 0.2 μM TPA Respectively. The following day, the cells were treated with the polysaccharide or polysaccharide mixture of Examples 1-1 to 7-3 and Comparative Examples 1-1 to 8-5 as test substances at a final concentration of 10 ppm or 50 ppm, and 0.1% DMSO , 100 μM IBMX as a positive control, and then cultured at 37 ° C. for 3 days. After incubation, the wells were washed with PBS, and 100 μl of 1N NaOH was added thereto, and melanin in the cells was dissolved. The absorbance of dissolved melanin was measured at 405 nm using a microplate reader. The results of comparing melanin production promoting effect of ginseng and green tea polysaccharide with the control group are shown in Table 10 below.

구분division 멜라닌 합성양(%)Amount of melanin synthesis (%) DMSO(0.1%)DMSO (0.1%) 100100 IBMX(100μM)IBMX (100 [mu] M) 120120 실시예 1-1Example 1-1 10ppm10ppm 110110 50ppm50 ppm 123123 실시예 1-2Examples 1-2 10ppm10ppm 111111 50ppm50 ppm 125125 실시예 1-3Example 1-3 10ppm10ppm 111111 50ppm50 ppm 126126 비교예 1-1Comparative Example 1-1 10ppm10ppm 107107 50ppm50 ppm 118118 비교예 1-2Comparative Example 1-2 10ppm10ppm 107107 50ppm50 ppm 119119 실시예 2-1Example 2-1 10ppm10ppm 112112 50ppm50 ppm 124124 실시예 2-2Example 2-2 10ppm10ppm 112112 50ppm50 ppm 125125 실시예 2-3Example 2-3 10ppm10ppm 113113 50ppm50 ppm 125125 비교예 2-1Comparative Example 2-1 10ppm10ppm 109109 50ppm50 ppm 119119 비교예 2-2Comparative Example 2-2 10ppm10ppm 110110 50ppm50 ppm 121121 실시예 3-1Example 3-1 10ppm10ppm 112112 50ppm50 ppm 126126 실시예 3-2Example 3-2 10ppm10ppm 112112 50ppm50 ppm 127127 실시예 3-3Example 3-3 10ppm10ppm 112112 50ppm50 ppm 125125 비교예 3-1Comparative Example 3-1 10ppm10ppm 110110 50ppm50 ppm 120120 비교예 3-2Comparative Example 3-2 10ppm10ppm 110110 50ppm50 ppm 119119 실시예 4-1Example 4-1 10ppm10ppm 112112 50ppm50 ppm 126126 실시예 4-2Example 4-2 10ppm10ppm 113113 50ppm50 ppm 128128 실시예 4-3Example 4-3 10ppm10ppm 113113 50ppm50 ppm 126126 비교예 4-1Comparative Example 4-1 10ppm10ppm 109109 50ppm50 ppm 118118 비교예 4-2Comparative Example 4-2 10ppm10ppm 109109 50ppm50 ppm 119119 실시예 5-1Example 5-1 10ppm10ppm 112112 50ppm50 ppm 124124 실시예 5-2Example 5-2 10ppm10ppm 112112 50ppm50 ppm 125125 실시예 5-3Example 5-3 10ppm10ppm 113113 50ppm50 ppm 127127 비교예 5-1Comparative Example 5-1 10ppm10ppm 109109 50ppm50 ppm 117117 비교예 5-2Comparative Example 5-2 10ppm10ppm 110110 50ppm50 ppm 120120 실시예 6-1Example 6-1 10ppm10ppm 111111 50ppm50 ppm 124124 실시예 6-2Example 6-2 10ppm10ppm 111111 50ppm50 ppm 126126 실시예 6-3Example 6-3 10ppm10ppm 113113 50ppm50 ppm 128128 비교예 6-1Comparative Example 6-1 10ppm10ppm 108108 50ppm50 ppm 119119 비교예 6-2Comparative Example 6-2 10ppm10ppm 109109 50ppm50 ppm 119119 실시예 7-1Example 7-1 10ppm10ppm 113113 50ppm50 ppm 126126 실시예 7-2Example 7-2 10ppm10ppm 113113 50ppm50 ppm 125125 실시예 7-3Example 7-3 10ppm10ppm 113113 50ppm50 ppm 125125 비교예 7-1Comparative Example 7-1 10ppm10ppm 111111 50ppm50 ppm 120120 비교예 7-2Comparative Example 7-2 10ppm10ppm 109109 50ppm50 ppm 118118 비교예 8-1Comparative Example 8-1 10ppm10ppm 105105 50ppm50 ppm 117117 비교예 8-2Comparative Example 8-2 10ppm10ppm 106106 50ppm50 ppm 119119 비교예 8-3Comparative Example 8-3 10ppm10ppm 109109 50ppm50 ppm 121121 비교예 8-4Comparative Example 8-4 10ppm10ppm 110110 50ppm50 ppm 122122 비교예 8-5Comparative Example 8-5 10ppm10ppm 111111 50ppm50 ppm 122122

상기 표 10을 보면, 인삼 다당체와 녹차 다당체가 멜라노사이트의 멜라닌 합성을 촉진시키며, 특히 포제 처리한 인삼 및 녹차로부터 추출한 다당체를 혼합하여 처리한 경우에 멜라닌 생성이 현저하게 증가하여, 우수한 멜라닌 생성 촉진 효과를 나타냄을 알 수 있다.
Table 10 shows that the ginseng polysaccharide and the green tea polysaccharide promote the melanin synthesis of melanocytes and the melanin production is remarkably increased when mixed with polysaccharides extracted from forage-treated ginseng and green tea to promote excellent melanin production Effect.

[시험예 6] 멜라노사이트에서의 MITF 및 티로시나제 발현 촉진 효과Test Example 6: Promotion of MITF and tyrosinase expression in melanocytes

501mel 세포주를 이용하여 6웰플레이트(6-well microtiter plate)에 500,000세포/웰이 되도록 분주하고, 각 웰에 음성 대조군으로는 DMSO 0.1%, 양성 대조군으로는 IBMX 100μM, 그리고 시험군으로는 실시예 1-1 내지 7-3 및 비교예 1-1 내지 8-5의 다당체 또는 다당체 혼합물을 10ppm으로 처리하여, 37℃ 온도에서 24시간, 48시간, 72시간 동안 배양한 후 단백질을 얻었다. 이렇게 얻은 단백질에 대하여 MITF 및 티로시나제 항체를 이용하여 웨스턴 블랏을 하였다. 단백질 추출과 웨스턴 블랏은 통상적으로 당업자가 사용하는 표준 방법으로 수행하였다. 웨스턴 블랏 후 그 결과를 음성 대조군을 100으로 하고 이 값과 비교하여 하기 표 11 및 12에 나타내었다. The cells were divided into six wells (6-well microtiter plate) to a concentration of 500,000 cells / well using a 501mel cell line. DMSO 0.1% as a negative control, IBMX 100 μM as a positive control, 1-1 to 7-3 and Comparative Examples 1-1 to 8-5 were treated at 10 ppm and cultured at 37 ° C for 24 hours, 48 hours and 72 hours to obtain proteins. The proteins thus obtained were subjected to western blotting using MITF and tyrosinase antibodies. Protein extraction and Western blotting were routinely performed by standard methods used by those skilled in the art. After Western blotting, the result was compared with the negative control group of 100, and the results are shown in Tables 11 and 12 below.

MITF 항체를 이용한 웨스턴 블랏Western blot using MITF antibody 구분division 24시간24 hours 48시간48 hours 72시간72 hours IBMXIBMX 119119 9797 212212 실시예 1-1Example 1-1 8686 118118 156156 실시예 1-2Examples 1-2 8686 115115 152152 실시예 1-3Example 1-3 8484 112112 150150 비교예 1-1Comparative Example 1-1 7979 112112 144144 비교예 1-2Comparative Example 1-2 7777 109109 142142 실시예 2-1Example 2-1 8787 117117 158158 실시예 2-2Example 2-2 8686 115115 153153 실시예 2-3Example 2-3 8686 115115 154154 비교예 2-1Comparative Example 2-1 7878 110110 145145 비교예 2-2Comparative Example 2-2 8080 112112 148148 실시예 3-1Example 3-1 8888 120120 158158 실시예 3-2Example 3-2 8787 118118 156156 실시예 3-3Example 3-3 8484 112112 151151 비교예 3-1Comparative Example 3-1 8181 114114 148148 비교예 3-2Comparative Example 3-2 7878 109109 145145 실시예 4-1Example 4-1 8787 118118 157157 실시예 4-2Example 4-2 8787 116116 155155 실시예 4-3Example 4-3 8585 113113 152152 비교예 4-1Comparative Example 4-1 8080 112112 146146 비교예 4-2Comparative Example 4-2 7979 110110 144144 실시예 5-1Example 5-1 8787 117117 156156 실시예 5-2Example 5-2 8686 115115 155155 실시예 5-3Example 5-3 8484 112112 150150 비교예 5-1Comparative Example 5-1 7878 108108 145145 비교예 5-2Comparative Example 5-2 7979 110110 147147 실시예 6-1Example 6-1 8787 119119 159159 실시예 6-2Example 6-2 8585 116116 155155 실시예 6-3Example 6-3 8383 112112 151151 비교예 6-1Comparative Example 6-1 8080 111111 145145 비교예 6-2Comparative Example 6-2 7878 108108 143143 실시예 7-1Example 7-1 8686 119119 158158 실시예 7-2Example 7-2 8585 115115 154154 실시예 7-3Example 7-3 8585 116116 154154 비교예 7-1Comparative Example 7-1 8181 112112 145145 비교예 7-2Comparative Example 7-2 8080 111111 144144 비교예 8-1Comparative Example 8-1 7878 110110 148148 비교예 8-2Comparative Example 8-2 7676 106106 142142 비교예 8-3Comparative Example 8-3 8686 117117 154154 비교예 8-4Comparative Example 8-4 8484 114114 150150 비교예 8-5Comparative Example 8-5 8080 111111 148148

티로시나제 항체를 이용한 웨스턴 블랏Western blot using tyrosinase antibody 구분division 24시간24 hours 48시간48 hours 72시간72 hours IBMXIBMX 155155 147147 287287 실시예 1-1Example 1-1 143143 174174 201201 실시예 1-2Examples 1-2 142142 174174 200200 실시예 1-3Example 1-3 140140 170170 195195 비교예 1-1Comparative Example 1-1 138138 165165 190190 비교예 1-2Comparative Example 1-2 136136 164164 188188 실시예 2-1Example 2-1 144144 177177 205205 실시예 2-2Example 2-2 142142 174174 198198 실시예 2-3Example 2-3 143143 175175 200200 비교예 2-1Comparative Example 2-1 139139 167167 192192 비교예 2-2Comparative Example 2-2 136136 164164 188188 실시예 3-1Example 3-1 142142 174174 198198 실시예 3-2Example 3-2 142142 175175 199199 실시예 3-3Example 3-3 143143 175175 201201 비교예 3-1Comparative Example 3-1 138138 166166 191191 비교예 3-2Comparative Example 3-2 138138 166166 193193 실시예 4-1Example 4-1 140140 171171 195195 실시예 4-2Example 4-2 141141 173173 196196 실시예 4-3Example 4-3 143143 174174 200200 비교예 4-1Comparative Example 4-1 137137 163163 190190 비교예 4-2Comparative Example 4-2 138138 164164 190190 실시예 5-1Example 5-1 141141 172172 194194 실시예 5-2Example 5-2 142142 174174 196196 실시예 5-3Example 5-3 143143 174174 199199 비교예 5-1Comparative Example 5-1 136136 162162 188188 비교예 5-2Comparative Example 5-2 139139 166166 190190 실시예 6-1Example 6-1 142142 172172 198198 실시예 6-2Example 6-2 141141 172172 196196 실시예 6-3Example 6-3 143143 174174 200200 비교예 6-1Comparative Example 6-1 137137 165165 191191 비교예 6-2Comparative Example 6-2 137137 165165 190190 실시예 7-1Example 7-1 142142 173173 196196 실시예 7-2Example 7-2 140140 171171 194194 실시예 7-3Example 7-3 143143 176176 202202 비교예 7-1Comparative Example 7-1 139139 163163 190190 비교예 7-2Comparative Example 7-2 137137 165165 189189 비교예 8-1Comparative Example 8-1 125125 154154 176176 비교예 8-2Comparative Example 8-2 127127 157157 184184 비교예 8-3Comparative Example 8-3 134134 160160 188188 비교예 8-4Comparative Example 8-4 135135 162162 191191 비교예 8-5Comparative Example 8-5 138138 167167 193193

상기 표 11 및 12를 보면, 인삼 다당체와 녹차 다당체는 멜라노사이트에서 MITF와 티로시나제 단백질 발현을 상승시키며, 특히 포제 처리한 인삼 및 녹차로부터 추출한 다당체를 혼합하여 처리한 경우에 MITF와 티로시나제 단백질 발현을 상승시키는 효과가 매우 우수함을 확인할 수 있다.
As shown in Tables 11 and 12, the ginseng polysaccharide and green tea polysaccharide increase the expression of MITF and tyrosinase protein in melanocytes. Especially, when mixed with polysaccharides extracted from forage-treated ginseng and green tea, the expression of MITF and tyrosinase protein increases It is possible to confirm that the effect of making the film is very excellent.

[참고예 2] 제형예 8-1 내지 14-3 및 비교제형예 10-1 내지 18의 제조[Referential Example 2] Preparation of Formulation Examples 8-1 to 14-3 and Comparative Formulation Examples 10-1 to 18

상기 실시예 1-1 내지 7-3 및 비교예 1-1 내지 8-5를 사용하여 하기 표 13 내지 표 17의 조성에 따라 두피 에센스를 제조하였다. 보다 구체적으로는 실시예 1-1 내지 7-3 및 비교예 1-1 내지 8-5의 효능 물질, 폴리옥시에틸렌 수소화된 피마자유(Polyoxyethylene Hydrogenated Castor Oils)/ POE(60) (Emalex HC-60, Nihon Emulsion Co.,Ltd.), 살리실릭산 0.2%, 에탄올 10%, 증류수, 암모늄아크릴로일디메틸타우레이트/브이피코폴리머(제조사: Aristoflex) 및 페녹시에탄올(Phenoxyethanol, PE)을 사용하였다.The scalp essences were prepared according to the compositions of Tables 13 to 17 using the above-described Examples 1-1 to 7-3 and Comparative Examples 1-1 to 8-5. More specifically, the efficacy materials of Examples 1-1 to 7-3 and Comparative Examples 1-1 to 8-5, polyoxyethylene hydrogenated castor oil / POE (60) (Emalex HC-60 , Nihon Emulsion Co., Ltd.), salicylic acid 0.2%, ethanol 10%, distilled water, ammonium acryloyldimethyltaurate / Vpicopolymer (manufacturer: Aristoflex) and phenoxyethanol (PE) .

(단위: 중량%)(Unit: wt%) 성분ingredient 제형예Formulation Example 8-18-1 8-28-2 8-38-3 9-19-1 9-29-2 9-39-3 10-110-1 10-210-2 10-310-3 폴리옥시에틸렌 수소화된 피마자유/ POE(60)Polyoxyethylene hydrogenated castor oil / POE (60) 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 실시예 1-1Example 1-1 0.20.2 -- -- -- -- -- -- 0.20.2 -- 실시예 1-2Examples 1-2 -- 0.20.2 -- -- -- -- -- -- -- 실시예 1-3Example 1-3 -- -- 0.20.2 -- -- -- -- -- -- 실시예 2-1Example 2-1 -- -- -- 0.20.2 -- -- -- -- -- 실시예 2-2Example 2-2 -- -- -- -- 0.20.2 -- -- -- -- 실시예 2-3Example 2-3 -- -- -- -- -- 0.20.2 -- -- -- 실시예 3-1Example 3-1 -- -- -- -- -- -- 0.20.2 -- -- 실시예 3-2Example 3-2 -- -- -- -- -- -- -- 0.20.2 -- 실시예 3-3Example 3-3 -- -- -- -- -- -- -- -- 0.20.2 살리실릭산Salicylic acid 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 에탄올ethanol 1010 1010 1010 1010 1010 1010 1010 1010 1010 증류수Distilled water 88.388.3 88.388.3 88.388.3 88.388.3 88.388.3 88.388.3 88.388.3 88.388.3 88.388.3 암모늄아크릴로일디메틸타우레이트/브이피코폴리머Ammonium acryloyldimethyltaurate / Vpicopolymer 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 페녹시에탄올Phenoxyethanol 0.30.3 0.30.3 0.30.3 0.30.3 0.30.3 0.30.3 0.30.3 0.30.3 0.30.3

(단위: 중량%)(Unit: wt%) 성분ingredient 제형예Formulation Example 11-111-1 11-211-2 11-311-3 12-112-1 12-212-2 12-312-3 13-113-1 13-213-2 13-313-3 폴리옥시에틸렌 수소화된 피마자유/ POE(60)Polyoxyethylene hydrogenated castor oil / POE (60) 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 실시예 4-1Example 4-1 0.20.2 -- -- -- -- -- -- 0.20.2 -- 실시예 4-2Example 4-2 -- 0.20.2 -- -- -- -- -- -- -- 실시예 4-3Example 4-3 -- -- 0.20.2 -- -- -- -- -- -- 실시예 5-1Example 5-1 -- -- -- 0.20.2 -- -- -- -- -- 실시예 5-2Example 5-2 -- -- -- -- 0.20.2 -- -- -- -- 실시예 5-3Example 5-3 -- -- -- -- -- 0.20.2 -- -- -- 실시예 6-1Example 6-1 -- -- -- -- -- -- 0.20.2 -- -- 실시예 6-2Example 6-2 -- -- -- -- -- -- -- 0.20.2 -- 실시예 6-3Example 6-3 -- -- -- -- -- -- -- -- 0.20.2 살리실릭산Salicylic acid 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 에탄올ethanol 1010 1010 1010 1010 1010 1010 1010 1010 1010 증류수Distilled water 88.388.3 88.388.3 88.388.3 88.388.3 88.388.3 88.388.3 88.388.3 88.388.3 88.388.3 암모늄아크릴로일디메틸타우레이트/브이피코폴리머Ammonium acryloyldimethyltaurate / Vpicopolymer 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 페녹시에탄올Phenoxyethanol 0.30.3 0.30.3 0.30.3 0.30.3 0.30.3 0.30.3 0.30.3 0.30.3 0.30.3

(단위: 중량%)(Unit: wt%) 성분ingredient 제형예Formulation Example 비교제형예Comparative Formulation Example 14-114-1 14-214-2 14-314-3 10-110-1 10-210-2 11-111-1 11-211-2 12-112-1 12-212-2 폴리옥시에틸렌 수소화된 피마자유/ POE(60)Polyoxyethylene hydrogenated castor oil / POE (60) 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 실시예 7-1Example 7-1 0.20.2 -- -- -- -- -- -- 0.20.2 -- 실시예 7-2Example 7-2 -- 0.20.2 -- -- -- -- -- -- -- 실시예 7-3Example 7-3 -- -- 0.20.2 -- -- -- -- -- -- 비교예 1-1Comparative Example 1-1 -- -- -- 0.20.2 -- -- -- -- -- 비교예 1-2Comparative Example 1-2 -- -- -- -- 0.20.2 -- -- -- -- 비교예 2-1Comparative Example 2-1 -- -- -- -- -- 0.20.2 -- -- -- 비교예 2-2Comparative Example 2-2 -- -- -- -- -- -- 0.20.2 -- -- 비교예 3-1Comparative Example 3-1 -- -- -- -- -- -- -- 0.20.2 -- 비교예 3-2Comparative Example 3-2 -- -- -- -- -- -- -- -- 0.20.2 살리실릭산Salicylic acid 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 에탄올ethanol 1010 1010 1010 1010 1010 1010 1010 1010 1010 증류수Distilled water 88.388.3 88.388.3 88.388.3 88.388.3 88.388.3 88.388.3 88.388.3 88.388.3 88.388.3 암모늄아크릴로일디메틸타우레이트/브이피코폴리머Ammonium acryloyldimethyltaurate / Vpicopolymer 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 페녹시에탄올Phenoxyethanol 0.30.3 0.30.3 0.30.3 0.30.3 0.30.3 0.30.3 0.30.3 0.30.3 0.30.3

(단위: 중량%)(Unit: wt%) 성분ingredient 비교제형예Comparative Formulation Example 13-113-1 13-213-2 14-114-1 14-214-2 15-115-1 15-215-2 16-116-1 16-216-2 폴리옥시에틸렌 수소화된 피마자유/ POE(60)Polyoxyethylene hydrogenated castor oil / POE (60) 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 비교예 4-1Comparative Example 4-1 0.20.2 -- -- -- -- -- -- 0.20.2 비교예 4-2Comparative Example 4-2 -- 0.20.2 -- -- -- -- -- -- 비교예 5-1Comparative Example 5-1 -- -- 0.20.2 -- -- -- -- -- 비교예 5-2Comparative Example 5-2 -- -- -- 0.20.2 -- -- -- -- 비교예 6-1Comparative Example 6-1 -- -- -- -- 0.20.2 -- -- -- 비교예 6-2Comparative Example 6-2 -- -- -- -- -- 0.20.2 -- -- 비교예 7-1Comparative Example 7-1 -- -- -- -- -- -- 0.20.2 -- 비교예 7-2Comparative Example 7-2 -- -- -- -- -- -- -- 0.20.2 살리실릭산Salicylic acid 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 에탄올ethanol 1010 1010 1010 1010 1010 1010 1010 1010 증류수Distilled water 88.388.3 88.388.3 88.388.3 88.388.3 88.388.3 88.388.3 88.388.3 88.388.3 암모늄아크릴로일디메틸타우레이트/브이피코폴리머Ammonium acryloyldimethyltaurate / Vpicopolymer 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 페녹시에탄올Phenoxyethanol 0.30.3 0.30.3 0.30.3 0.30.3 0.30.3 0.30.3 0.30.3 0.30.3

(단위: 중량%)(Unit: wt%) 성분ingredient 비교제형예Comparative Formulation Example 17-117-1 17-217-2 17-317-3 17-417-4 17-517-5 1818 폴리옥시에틸렌 수소화된 피마자유/ POE(60)Polyoxyethylene hydrogenated castor oil / POE (60) 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 비교예 8-1Comparative Example 8-1 0.20.2 -- -- -- -- -- 비교예 8-2Comparative Example 8-2 -- 0.20.2 -- -- -- -- 비교예 8-3Comparative Example 8-3 -- -- 0.20.2 -- -- -- 비교예 8-4Comparative Example 8-4 -- -- -- 0.20.2 -- -- 비교예 8-5Comparative Example 8-5 -- -- -- -- 0.20.2 -- 살리실릭산Salicylic acid 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 0.20.2 에탄올ethanol 1010 1010 1010 1010 1010 1010 증류수Distilled water 88.388.3 88.388.3 88.388.3 88.388.3 88.388.3 88.388.3 암모늄아크릴로일디메틸타우레이트/브이피코폴리머Ammonium acryloyldimethyltaurate / Vpicopolymer 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 0.50.5 페녹시에탄올Phenoxyethanol 0.30.3 0.30.3 0.30.3 0.30.3 0.30.3 0.30.3

[시험예 7] 피부 자극 시험[Test Example 7] Skin irritation test

본 발명에 따른 조성물의 사용시 피부 자극 여부를 확인하기 위하여 비교적 두피 가려움을 심하게 느끼는 25~45세의 남녀 410명을 선정하여 10명씩 41개 그룹으로 나누고 각 그룹에 대하여 제형예 8-1 내지 14-3 및 비교제형예 10-1 내지 18의 두피 에센스를 사용하도록 하였다. 구체적으로는, 등피부를 씻은 후에 물기를 닦아내고, 항온항습실(온도 24 ± 4℃, 상대습도 40~45%)에서 15분간 대기하도록 하였다. 효능 물질을 0.2% 함유한 두피 에센스를 각각 흐르지 않을 정도의 동량을 탈지면에 충분히 도포하여 등 피부의 위치 중 정해서 부착하였다. 시험물질을 부착하고 24시간과 48시간 경과 후 피험자가 자극의 정도를 0점에서 4점(0= 자극 없음, 1= 아주 약함, 2 = 약함, 3 = 중간, 4 = 심함)까지로 표시하였으며(총 2회 측정), 따가움, 화끈거림 등 자극감에 대하여 평가하였다. In order to confirm whether or not the skin irritation occurred when using the composition according to the present invention, 410 men and women aged 25 to 45 years who were relatively scarred with scalp itching were selected and divided into 41 groups of 10 persons. Formulations 8-1 to 14- 3 and Comparative Formulations 10-1 to 18 were used. Concretely, after washing the cheek piece, the water was wiped off and the sample was allowed to stand in a constant temperature and humidity room (temperature 24 ± 4 ° C., relative humidity 40 to 45%) for 15 minutes. The same amount of the scalp essence containing 0.2% of the efficacious substance was applied to the skin surface to the extent that did not allow the skin essence to flow therethrough. After 24 hours and 48 hours after the test substance was attached, the subject marked the degree of stimulation from 0 to 4 points (0 = no stimulation, 1 = very weak, 2 = weak, 3 = moderate, 4 = severe) (2 measurements in total), stinging, and burning sensation.

각 시간대별로 상기 등급에 따른 자극을 호소한 피험자의 숫자를 하기 표 18에 나타내었다. 또한, 피부 자극 여부를 평균 수치(Mean score)로 도출하기 위해 평균값을 계산하고, 결과를 하기 표 18에 나타내었다.The number of subjects who complained of the above-mentioned stimulus for each time slot is shown in Table 18 below. The average value was calculated to derive the skin irritation as a mean score, and the results are shown in Table 18 below.

시험물질명Test material name 시험 농도Test concentration 24hr24hr 48hr48hr 반응인원Responses 판정Judgment 1One 22 33 44 1One 22 33 44 (n=10)(n = 10) 제형예 8-1Formulation Example 8-1 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 제형예 8-2Formulation Example 8-2 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 제형예 8-3Formulation Example 8-3 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 비교제형예 10-1Comparative Formulation Example 10-1 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 비교제형예 10-2Comparative Formulation Example 10-2 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 제형예 9-1Formulation Example 9-1 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 제형예 9-2Formulation Example 9-2 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 제형예 9-3Formulation Example 9-3 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 비교제형예 11-1Comparative Formulation Example 11-1 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 비교제형예 11-2Comparative Formulation Example 11-2 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 제형예 10-1Formulation Example 10-1 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 제형예 10-2Formulation Example 10-2 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 제형예 10-3Formulation Example 10-3 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 비교제형예 12-1Comparative Formulation Example 12-1 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 비교제형예 12-2Comparative Formulation Example 12-2 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 제형예 11-1Formulation Example 11-1 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 제형예 11-2Formulation Example 11-2 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 제형예 11-3Formulation Example 11-3 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 비교제형예 13-1Comparative Formulation Example 13-1 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 비교제형예 13-2Comparative Formulation Example 13-2 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 제형예 12-1Formulation Example 12-1 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 제형예 12-2Formulation Example 12-2 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 제형예 12-3Formulation Example 12-3 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 비교제형예 14-1Comparative Formulation Example 14-1 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 비교제형예 14-2Comparative Formulation Example 14-2 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 제형예 13-1Formulation Example 13-1 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 제형예 13-2Formulation Example 13-2 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 제형예 13-3Formulation Example 13-3 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 비교제형예 15-1Comparative Formulation Example 15-1 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 비교제형예 15-2Comparative Formulation Example 15-2 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 제형예 14-1Formulation Example 14-1 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 제형예 14-2Formulation Example 14-2 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 제형예 14-3Formulation Example 14-3 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 비교제형예 16-1Comparative Formulation Example 16-1 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 비교제형예 16-2Comparative Formulation Example 16-2 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 비교제형예 17-1Comparative Formulation Example 17-1 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 비교제형예 17-2Comparative Formulation Example 17-2 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 비교제형예 17-3Comparative Formulation Example 17-3 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 비교제형예 17-4Comparative Formulation Example 17-4 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 비교제형예 17-5Comparative Formulation Examples 17-5 0.20%0.20% 1One 00 00 00 00 00 00 00 1One 자극없음No stimulation 비교제형예 18Comparative Formulation Example 18 0.20%0.20% 00 00 00 00 1One 00 00 00 1One 자극없음No stimulation

상기 표 18의 결과에서, 제형예 8-1 내지 14-3 및 비교제형예 10-1 내지 17-5의 다당체 또는 다당체 혼합물을 포함하는 조성물은 다당체를 포함하지 않는 비교제형예 18과 동일하게 피부 자극이 없음을 확인할 수 있었다. 따라서, 본 발명에 따른 조성물은 인삼 및 녹차 유래의 다당체를 유효성분으로 사용함으로써 인체에 안전하고 피부에 자극이 없으며, 장기간 사용하여도 부작용이 없음을 알 수 있다.
In the results of Table 18 above, the compositions containing the polysaccharide or polysaccharide mixture of Formulation Examples 8-1 to 14-3 and Comparative Formulation Examples 10-1 to 17-5 were obtained in the same manner as Comparative Formulation Example 18 containing no polysaccharide It was confirmed that there was no stimulation. Therefore, it can be seen that the composition according to the present invention is safe to the human body and has no irritation to the skin by using the polysaccharide derived from ginseng and green tea as an active ingredient, and has no side effects even when it is used for a long time.

[참고예 3] 제형예 15-1 내지 21-3 및 비교제형예 19-1 내지 27의 제조[Reference Example 3] Preparation of Formulation Examples 15-1 to 21-3 and Comparative Formulation Examples 19-1 to 27

상기 실시예 1-1 내지 7-3 및 비교예 1-1 내지 8-5를 사용하여 하기 표 19 내지 23의 조성에 따라 두피 및 모발 겸용 트리트먼트 조성물을 제조하였다. 유상과 수상을 배합기에 따로 준비한 후, 유상을 수상에 서서히 첨가하고 호모믹서를 이용하여 3,000rpm으로 10분간 유화시킨 후 실온까지 냉각시켜 제형예 15-1 내지 21-3 및 비교제형예 19-1 내지 27의 두피 및 모발 겸용 트리트먼트를 제조하였다.Using the above-mentioned Examples 1-1 to 7-3 and Comparative Examples 1-1 to 8-5, a scalp and hair combination treatment composition was prepared according to the compositions of Tables 19 to 23 below. The oil phase and water phase were separately prepared in a blender, the oil phase was gradually added to the water phase, emulsified at 3,000 rpm for 10 minutes using a homomixer, and cooled to room temperature to obtain Formulation Examples 15-1 to 21-3 and Comparative Formulation Examples 19-1 To 27 < / RTI > of scalp and hair combs were prepared.

성분 (중량%)Component (% by weight) 제형예Formulation Example 15-115-1 15-215-2 15-315-3 16-116-1 16-216-2 16-316-3 17-117-1 17-217-2 17-317-3 정제수Purified water 잔액
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실시예 1-1Example 1-1 0.50.5 -- -- -- -- -- -- -- -- 실시예 1-2Examples 1-2 -- 0.50.5 -- -- -- -- -- -- -- 실시예 1-3Example 1-3 -- -- 0.50.5 -- -- -- -- -- -- 실시예 2-1Example 2-1 -- -- -- 0.50.5 -- -- -- -- -- 실시예 2-2Example 2-2 -- -- -- -- 0.50.5 -- -- -- -- 실시예 2-3Example 2-3 -- -- -- -- -- 0.50.5 -- -- -- 실시예 3-1Example 3-1 -- -- -- -- -- -- 0.50.5 -- -- 실시예 3-2Example 3-2 -- -- -- -- -- -- -- 0.50.5 -- 실시예 3-3Example 3-3 -- -- -- -- -- -- -- -- 0.50.5 세틸트리메틸암모늄클로라이드Cetyltrimethylammonium chloride 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 세틸알코올Cetyl alcohol 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 스테아릴알코올Stearyl alcohol 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 방부제, 색소 및 향료Preservatives, coloring and flavoring 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount

성분 (중량%)Component (% by weight) 제형예Formulation Example 18-118-1 18-218-2 18-318-3 19-119-1 19-219-2 19-319-3 20-120-1 20-220-2 20-320-3 정제수Purified water 잔액
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실시예 4-1Example 4-1 0.50.5 -- -- -- -- -- -- -- -- 실시예 4-2Example 4-2 -- 0.50.5 -- -- -- -- -- -- -- 실시예 4-3Example 4-3 -- -- 0.50.5 -- -- -- -- -- -- 실시예 5-1Example 5-1 -- -- -- 0.50.5 -- -- -- -- -- 실시예 5-2Example 5-2 -- -- -- -- 0.50.5 -- -- -- -- 실시예 5-3Example 5-3 -- -- -- -- -- 0.50.5 -- -- -- 실시예 6-1Example 6-1 -- -- -- -- -- -- 0.50.5 -- -- 실시예 6-2Example 6-2 -- -- -- -- -- -- -- 0.50.5 -- 실시예 6-3Example 6-3 -- -- -- -- -- -- -- -- 0.50.5 세틸트리메틸암모늄클로라이드Cetyltrimethylammonium chloride 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 세틸알코올Cetyl alcohol 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 스테아릴알코올Stearyl alcohol 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 방부제, 색소 및 향료Preservatives, coloring and flavoring 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount

성분 (중량%)Component (% by weight) 제형예Formulation Example 비교제형예Comparative Formulation Example 21-121-1 21-221-2 21-321-3 19-119-1 19-219-2 20-120-1 20-220-2 21-121-1 21-221-2 정제수Purified water 잔액
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실시예 7-1Example 7-1 0.50.5 -- -- -- -- -- -- -- -- 실시예 7-2Example 7-2 -- 0.50.5 -- -- -- -- -- -- -- 실시예 7-3Example 7-3 -- -- 0.50.5 -- -- -- -- -- -- 비교예 1-1Comparative Example 1-1 -- -- -- 0.50.5 -- -- -- -- -- 비교예 1-2Comparative Example 1-2 -- -- -- -- 0.50.5 -- -- -- -- 비교예 2-1Comparative Example 2-1 -- -- -- -- -- 0.50.5 -- -- -- 비교예 2-2Comparative Example 2-2 -- -- -- -- -- -- 0.50.5 -- -- 비교예 3-1Comparative Example 3-1 -- -- -- -- -- -- -- 0.50.5 -- 비교예 3-2Comparative Example 3-2 -- -- -- -- -- -- -- -- 0.50.5 세틸트리메틸암모늄클로라이드Cetyltrimethylammonium chloride 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 세틸알코올Cetyl alcohol 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 스테아릴알코올Stearyl alcohol 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 방부제, 색소 및 향료Preservatives, coloring and flavoring 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount

성분 (중량%)Component (% by weight) 비교제형예Comparative Formulation Example 22-122-1 22-222-2 23-123-1 23-223-2 24-124-1 24-224-2 25-125-1 25-225-2 정제수Purified water 잔액
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비교예 4-1Comparative Example 4-1 0.50.5 -- -- -- -- -- -- -- 비교예 4-2Comparative Example 4-2 -- 0.50.5 -- -- -- -- -- -- 비교예 5-1Comparative Example 5-1 -- -- 0.50.5 -- -- -- -- -- 비교예 5-2Comparative Example 5-2 -- -- -- 0.50.5 -- -- -- -- 비교예 6-1Comparative Example 6-1 -- -- -- -- 0.50.5 -- -- -- 비교예 6-2Comparative Example 6-2 -- -- -- -- -- 0.50.5 -- -- 비교예 7-1Comparative Example 7-1 -- -- -- -- -- -- 0.50.5 -- 비교예 7-2Comparative Example 7-2 -- -- -- -- -- -- -- 0.50.5 세틸트리메틸암모늄클로라이드Cetyltrimethylammonium chloride 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 세틸알코올Cetyl alcohol 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 스테아릴알코올Stearyl alcohol 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 방부제, 색소 및 향료Preservatives, coloring and flavoring 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount

성분 (중량%)Component (% by weight) 비교제형예Comparative Formulation Example 26-126-1 26-226-2 26-326-3 26-426-4 26-526-5 2727 정제수Purified water 잔액
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balance
(to 100)
비교예 8-1Comparative Example 8-1 0.50.5 -- -- -- -- -- 비교예 8-2Comparative Example 8-2 -- 0.50.5 -- -- -- -- 비교예 8-3Comparative Example 8-3 -- -- 0.50.5 -- -- -- 비교예 8-4Comparative Example 8-4 -- -- -- 0.50.5 -- -- 비교예 8-5Comparative Example 8-5 -- -- -- -- 0.50.5 -- 세틸트리메틸암모늄클로라이드Cetyltrimethylammonium chloride 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 1.01.0 세틸알코올Cetyl alcohol 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 스테아릴알코올Stearyl alcohol 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 3.03.0 방부제, 색소 및 향료Preservatives, coloring and flavoring 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount 적량Suitable amount

[시험예 9] 관능시험[Test Example 9]

상기 제형예 15-1 내지 21-3 및 비교제형예 19-1 내지 27의 두피 및 모발 겸용 트리트먼트 조성물에 대하여 다음과 같은 방법으로 관능테스트를 진행하였다. 전문평가요원 410명을 10명씩 41개 그룹으로 나누고 각 그룹에 대하여 상기 제형예 15-1 내지 21-3 및 비교제형예 19-1 내지 27의 두피 및 모발 겸용 트리트먼트를 사용하게 한 후 하기의 표 24에 나타난 평가 항목에 대하여 비교 테스트를 하도록 하였으며, 가장 낮은 점수를 1로 하고 가장 높은 점수를 5로 하여 테스트를 진행하였다. 테스트 결과는 하기 표 24에 나타내었다.Sensory tests were carried out on the scalp and hair-combined treatment compositions of Formulation Examples 15-1 to 21-3 and Comparative Formulation Examples 19-1 to 27 by the following method. Forty-one 410 professional evaluation personnel were divided into 41 groups of 10, and the scalp and hair-combing treatments of Formulation Examples 15-1 to 21-3 and Comparative Formulation Examples 19-1 to 27 were used for each group, A comparison test was performed on the items shown in Table 24, and the test was conducted with the lowest score being 1 and the highest score being 5. The test results are shown in Table 24 below.

항목Item 두피 가려움Scalp itching 두피 자극감Scalp stimulus 모발 부드러움/매끄러움Hair softness / smoothness 제형예 15-1Formulation Example 15-1 2.22.2 2.22.2 2.52.5 제형예 15-2Formulation Example 15-2 2.12.1 2.02.0 2.62.6 제형예 15-3Formulation Example 15-3 2.12.1 2.12.1 2.72.7 비교제형예 19-1Comparative Formulation Example 19-1 2.52.5 2.42.4 2.32.3 비교제형예 19-2Comparative Formulation Example 19-2 2.32.3 2.42.4 2.42.4 제형예 16-1Formulation Example 16-1 2.12.1 2.22.2 2.72.7 제형예 16-2Formulation Example 16-2 2.02.0 2.02.0 2.82.8 제형예 16-3Formulation Example 16-3 2.02.0 2.02.0 2.92.9 비교제형예 20-1Comparative Formulation Example 20-1 2.42.4 2.52.5 2.42.4 비교제형예 20-2Comparative Formulation Example 20-2 2.22.2 2.62.6 2.72.7 제형예 17-1Formulation Example 17-1 1.91.9 1.91.9 2.82.8 제형예 17-2Formulation Example 17-2 1.91.9 2.02.0 2.72.7 제형예 17-3Formulation Example 17-3 2.12.1 2.12.1 2.62.6 비교제형예 21-1Comparative Formulation Example 21-1 2.22.2 2.32.3 2.52.5 비교제형예 21-2Comparative Formulation Example 21-2 2.42.4 2.52.5 2.32.3 제형예 18-1Formulation Example 18-1 2.02.0 2.12.1 2.62.6 제형예 18-2Formulation Example 18-2 2.02.0 2.02.0 2.72.7 제형예 18-3Formulation Example 18-3 2.12.1 2.12.1 2.72.7 비교제형예 22-1Comparative Formulation Example 22-1 2.32.3 2.52.5 2.32.3 비교제형예 22-2Comparative Formulation Example 22-2 2.52.5 2.62.6 2.22.2 제형예 19-1Formulation Example 19-1 1.81.8 1.81.8 2.92.9 제형예 19-2Formulation Example 19-2 1.91.9 2.02.0 2.72.7 제형예 19-3Formulation Example 19-3 2.02.0 2.22.2 2.62.6 비교제형예 23-1Comparative Formulation Example 23-1 2.42.4 2.52.5 2.32.3 비교제형예 23-2Comparative Formulation Example 23-2 2.22.2 2.52.5 2.32.3 제형예 20-1Formulation Example 20-1 2.12.1 2.12.1 2.92.9 제형예 20-2Formulation Example 20-2 2.22.2 2.22.2 2.82.8 제형예 20-3Formulation Example 20-3 2.12.1 2.22.2 2.92.9 비교제형예 24-1Comparative Formulation Example 24-1 2.62.6 2.52.5 2.32.3 비교제형예 24-2Comparative Formulation Example 24-2 2.42.4 2.42.4 2.42.4 제형예 21-1Formulation Example 21-1 2.22.2 2.32.3 2.92.9 제형예 21-2Formulation Example 21-2 2.32.3 2.52.5 2.82.8 제형예 21-3Formulation Example 21-3 2.22.2 2.32.3 2.92.9 비교제형예 25-1Comparative Formulation Example 25-1 2.42.4 2.62.6 2.62.6 비교제형예 25-2Comparative Formulation Example 25-2 2.22.2 2.52.5 2.62.6 비교제형예 26-1Comparative Formulation Example 26-1 3.33.3 3.13.1 2.12.1 비교제형예 26-2Comparative Formulation Example 26-2 3.13.1 3.03.0 2.32.3 비교제형예 26-3Comparative Formulation Example 26-3 3.03.0 2.92.9 2.22.2 비교제형예 26-4Comparative Formulation Example 26-4 3.03.0 3.03.0 2.42.4 비교제형예 26-5Comparative Formulation Examples 26-5 2.82.8 2.82.8 2.42.4 비교제형예 27Comparative Formulation Example 27 4.14.1 3.83.8 1.51.5

상기 표 24를 보면, 인삼 또는 녹차 다당체를 포함하는 제형예 15-1 내지 21-3 및 비교제형예 19-1 내지 26-5의 경우 다당체를 전혀 포함하지 않는 비교제형예 27을 사용한 경우와 비교하여 두피의 가려움이 감소되고 자극감도 완화되었으며, 또한 모발 상태도 보다 좋아짐을 알 수 있다. 특히, 포제 처리한 인삼 및 녹차로부터 추출한 다당체의 혼합물을 포함하는 제형예 15-1 내지 21-3을 사용하는 경우 그 효과가 매우 우수함을 확인할 수 있다.
Table 24 shows that Formulation Examples 15-1 to 21-3 containing ginseng or green tea polysaccharide and Comparative Formulation Examples 19-1 to 26-5 and Comparative Example 27 containing no polysaccharide were used , The itching of the scalp was reduced, the irritation was alleviated, and the hair condition was also improved. Particularly, the use of Formulation Examples 15-1 to 21-3 comprising a mixture of ginseng treated with fog and a polysaccharide extracted from green tea can be confirmed to be very excellent.

[제제예 1] 헤어 토닉 [Formulation Example 1] Hair tonic

하기 표 25에 기재된 조성에 따라 통상적인 방법으로 헤어 토닉을 제조하였다.Hair tonics were prepared in a conventional manner according to the composition shown in Table 25 below.

성분ingredient 중량비(%)Weight ratio (%) 에탄올ethanol 5050 멘톨menthol 0.020.02 글리세린glycerin 33 살리실산Salicylic acid 0.050.05 실시예 1-2의 다당체 혼합물The polysaccharide mixture of Example 1-2 1.01.0 향료 및 색소Spices and Pigments 적당량Proper amount 정제수Purified water 잔액 (to 100)Balance (to 100)

[제제예 2] 헤어 로션[Formulation Example 2] Hair lotion

하기 표 26에 기재된 조성에 따라 통상적인 방법으로 헤어 로션을 제조하였다.Hair lotions were prepared according to the composition shown in Table 26 below in a conventional manner.

성분ingredient 중량비(%)Weight ratio (%) 세토스테아릴알코올Cetostearyl alcohol 2.02.0 EDTA 2NaEDTA 2Na 0.20.2 히드록시에틸셀룰로오즈Hydroxyethyl cellulose 0.50.5 미네랄오일Mineral oil 5.05.0 실시예 1-2의 다당체 혼합물The polysaccharide mixture of Example 1-2 1.01.0 방부제antiseptic 적당량Proper amount 향료 및 색소Spices and Pigments 적당량Proper amount 정제수Purified water 잔액 (to 100)Balance (to 100)

[제제예 3] 모발 영양화장수[Formulation Example 3] Hair nutritional lotion

하기 표 27에 기재된 조성에 따라 통상적인 방법으로 모발 영양화장수를 제조하였다.A hair nutritional lotion was prepared by a conventional method according to the composition shown in Table 27 below.

성분ingredient 중량비(%)Weight ratio (%) 밀납Wax 4.04.0 폴리솔베이트 60Polysorbate 60 1.51.5 솔비탄세스퀴올레이트Sorbitan sesquioleate 0.70.7 미네랄 오일Mineral oil 10.010.0 글리세린glycerin 3.03.0 부틸렌글리콜Butylene glycol 3.03.0 프로필렌글리콜Propylene glycol 3.03.0 카르복시비닐폴리머Carboxyvinyl polymer 0.10.1 트리에탄올아민Triethanolamine 0.20.2 실시예 1-2의 다당체 혼합물The polysaccharide mixture of Example 1-2 1.01.0 방부제antiseptic 적당량Proper amount 향료 및 색소Spices and Pigments 적당량Proper amount 정제수Purified water 잔액 (to 100)Balance (to 100)

[제제예 4] 헤어샴푸 [Formulation Example 4] Hair shampoo

하기 표 28에 기재된 조성에 따라 통상적인 방법으로 헤어샴푸를 제조하였다.Hair shampoos were prepared in a conventional manner according to the composition shown in Table 28 below.

성분ingredient 중량%weight% 정제수Purified water 잔액 (To 100)Balance (To 100) 실시예 1-2의 다당체 혼합물The polysaccharide mixture of Example 1-2 1.01.0 소디윰라우릴설파이트Sodillauryl sulphite 36.036.0 코카미도프로필베타인Cocamidopropyl betaine 8.08.0 팔미티딘말레이트Palmitidine dimalate 2.02.0 글라이콜스테아레이트Glycollosteralate 1.51.5 폴리쿼터니윰 10Polyquaternium 10 0.50.5 사이트릭애씨드Saitric Acid 0.10.1 글리세린glycerin 2.02.0 방부제, 색소 및 향료Preservatives, coloring and flavoring 적량Suitable amount

[제제예 5] 헤어 린스[Formulation Example 5] Hair rinse

하기 표 29에 기재된 조성에 따라 통상적인 방법으로 헤어 린스를 제조하였다.The hair rinse was prepared according to a conventional method according to the composition shown in Table 29 below.

성분ingredient 중량%weight% 정제수Purified water 잔액 (To 100)Balance (To 100) 실시예 1-2의 다당체 혼합물The polysaccharide mixture of Example 1-2 1.01.0 프로필렌글라이콜Propylene glycol 2.02.0 세틸트리메틸암모늄클로라이드Cetyltrimethylammonium chloride 1.01.0 세틸알코올Cetyl alcohol 3.03.0 스테아릴알코올Stearyl alcohol 3.03.0 미네랄오일Mineral oil 0.50.5 사이트릭애씨드Saitric Acid 0.20.2 폴리디메틸실록산Polydimethylsiloxane 1.01.0 방부제, 색소 및 향료Preservatives, coloring and flavoring 적량Suitable amount

이상으로 본 발명 내용의 특정한 부분을 상세히 기술하였는 바, 당업계의 통상의 지식을 가진 자에게 있어서 이러한 구체적 기술은 단지 바람직한 실시태양일 뿐이며, 이에 의해 본 발명의 범위가 제한되는 것이 아닌 점은 명백할 것이다. 따라서, 본 발명의 실질적인 범위는 첨부된 청구항들과 그것들의 등가물에 의하여 정의된다고 할 것이다.While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will readily appreciate that many modifications are possible in the exemplary embodiments without materially departing from the novel teachings and advantages of this invention. something to do. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (15)

포제 처리한 인삼에서 추출한 다당체; 및 포제 처리한 녹차에서 추출한 다당체를 유효성분으로 함유하는 모발 또는 두피 상태 개선용 피부 외용제 조성물.Polysaccharides extracted from folate-treated ginseng; And a polysaccharide extracted from a green tea treated as an active ingredient. 제1항에 있어서, 상기 포제 처리한 인삼에서 추출한 다당체와 포제 처리한 녹차에서 추출한 다당체는 각각 조성물 총 중량에 대하여 0.001~10중량%의 양으로 함유되는 피부 외용제 조성물.2. The composition for external application for skin according to claim 1, wherein the polysaccharide extracted from the fomed ginseng and the polysaccharide extracted from the fomed green tea are contained in an amount of 0.001-10 wt% based on the total weight of the composition. 제1항에 있어서, 상기 포제 처리한 인삼에서 추출한 다당체와 포제 처리한 녹차에서 추출한 다당체는 중량비로 0.1~10:1의 비율로 혼합사용되는 피부 외용제 조성물.The composition for external application for skin according to claim 1, wherein the polysaccharide extracted from the fomed ginseng and the polysaccharide extracted from the green tea are mixed at a ratio of 0.1 to 10: 1 by weight. 제1항에 있어서, 상기 조성물은 비듬 방지용임을 특징으로 하는 피부 외용제 조성물.The composition for external application for skin according to claim 1, wherein the composition is for preventing dandruff. 제1항에 있어서, 상기 조성물은 육모용임을 특징으로 하는 피부 외용제 조성물.The composition for external application for skin according to claim 1, wherein the composition is for hair growth. 제1항에 있어서, 상기 조성물은 백모 방지용임을 특징으로 하는 피부 외용제 조성물.The composition for external application for skin according to claim 1, wherein the composition is for prevention of white hair. 제1항에 있어서, 상기 조성물은 두피 자극 완화용임을 특징으로 하는 피부 외용제 조성물.The composition for external application for skin according to claim 1, wherein the composition is for alleviating scalp irritation. 제1항에 있어서, 상기 조성물은 모발 부드러움 개선용임을 특징으로 하는 피부 외용제 조성물.The composition for external application for skin according to claim 1, wherein the composition is for improving hair softness. 포제 처리한 인삼에서 추출한 다당체; 및 포제 처리한 녹차에서 추출한 다당체를 유효성분으로 함유하는 모발 또는 두피 상태 개선용 약학 조성물.Polysaccharides extracted from folate-treated ginseng; And a polysaccharide extracted from a green tea treated with a fogging agent as an active ingredient. 제9항에 있어서, 상기 포제 처리한 인삼에서 추출한 다당체와 포제 처리한 녹차에서 추출한 다당체는 각각 조성물 총 중량에 대하여 0.001~10중량%의 양으로 함유되는 약학 조성물.[Claim 11] The pharmaceutical composition according to claim 9, wherein the polysaccharide extracted from the fomed ginseng and the polysaccharide extracted from the green tea are contained in an amount of 0.001-10 wt% based on the total weight of the composition. 제9항에 있어서, 상기 포제 처리한 인삼에서 추출한 다당체와 포제 처리한 녹차에서 추출한 다당체는 중량비로 0.1~10:1의 비율로 혼합사용되는 약학 조성물.[Claim 10] The pharmaceutical composition according to claim 9, wherein the polysaccharide extracted from the fungus-treated ginseng and the polysaccharide extracted from the green tea are mixed at a ratio of 0.1 to 10: 1 by weight. 제9항에 있어서, 상기 조성물은 비듬 방지용임을 특징으로 하는 약학 조성물.10. The pharmaceutical composition according to claim 9, wherein the composition is for preventing dandruff. 제9항에 있어서, 상기 조성물은 육모용임을 특징으로 하는 약학 조성물.10. The pharmaceutical composition of claim 9, wherein the composition is hair tonic. 제9항에 있어서, 상기 조성물은 백모 방지용임을 특징으로 하는 약학 조성물.10. The pharmaceutical composition according to claim 9, wherein said composition is for preventing white hair. 제9항에 있어서, 상기 조성물은 두피 자극 완화용임을 특징으로 하는 약학 조성물.10. The pharmaceutical composition according to claim 9, wherein said composition is for alleviating scalp irritation.
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