KR20150036917A - Composition for improving condition of hair and scalp containing polysaccharides extracted from processed Panax ginseng and Green tea - Google Patents

Abstract

The present invention relates to a composition including a ginseng polysaccharide and a green tea polysaccharide for improvement of hair or scalp conditions. More specifically, the present invention relates to a composition which uses a mixture obtained by mixing polysaccharides extracted from an extract of preprocessed ginseng and green tea. Accordingly, the composition prevents grey hair and hair loss, alleviates scalp irritation and inhibits dandruff, scalp inflammation, etc, thereby improving general hair conditions and having an excellent cleaning and calming effect on the scalp. The composition includes the ginseng polysaccharide and the green tea polysaccharide separately in a content of 0.001-10 wt% with respect to the total weight of the composition. The ginseng polysaccharide and the green tea polysaccharide are mixed in the weight ratio of 0.1-10:1.

Description

TECHNICAL FIELD The present invention relates to a composition for improving the condition of hair or scalp containing polysaccharides extracted from green tea,

The present invention relates to a hair or scalp condition improving composition containing a ginseng polysaccharide and a green tea polysaccharide. More particularly, the present invention relates to a composition for preventing hair loss and hair loss by mixing a polysaccharide extracted from an extract of green tea, , A composition which alleviates irritation to the scalp, suppresses dandruff and scalp inflammation, thereby improving the overall hair condition, and having a cleansing and soothing effect on the scalp.

The scalp is composed of the epidermis, dermis, and subcutaneous tissue, and the outermost tissue is the epidermis. The epidermis protects the body's sensitive tissues from damage. However, due to various physical and chemical external stimuli such as external temperature, humidity change, pollutants, stress, and lack of influence, the scalp function may be lost, and furthermore, it may affect hair loss.

In modern society, which emphasizes beauty, many people, both men and women, use hair dye, permanent hair, and the like in order to satisfy the need for expression of individuality. However, in the case of some sensitive scalp, chemical treatment such as dyeing and perming causes irritation such as itching or stinging of the scalp and inflammation, which leads to environmental hair loss.

In this way, it is possible to protect the scalp from stimulation, stress, and chemical treatment from the environment due to various causes, to promote hair growth while preventing hair loss, and to continue research on ecosystem-friendly and environmentally harmless components to humans .

Therefore, the inventors of the present invention have found that when ginseng and green tea are subjected to forage treatment and polysaccharide is extracted therefrom, a substance enhanced in potency can be obtained as compared with ginseng or green tea which has not been treated with faujasite, and the obtained ginseng polysaccharide and green tea polysaccharide are simultaneously used The present inventors have found that the polysaccharides are more effective than the polysaccharides for preventing white hair, hair loss, and dandruff, respectively, by the synergistic effect, and completed the present invention.

Accordingly, it is an object of the present invention to provide a composition exhibiting excellent hair or scalp condition improving effect by simultaneously using ginseng polysaccharide extracted from forage-treated ginseng and green tea extract and green tea polysaccharide.

In order to achieve the above object, the present invention provides a composition for external application for skin or scalp or a pharmaceutical composition comprising the ginseng polysaccharide extracted from the extract of green tea and the green tea polysaccharide as an active ingredient.

The composition of the present invention can obtain a substance enhanced in efficacy by extracting a polysaccharide from a ginseng and green tea extract that has been subjected to forage treatment. It also contains ginseng polysaccharide and green tea polysaccharide simultaneously to prevent white hair and hair loss, Not only is it effective in preventing scalp itching and dandruff, it also makes the hair softer and shiny.

The composition of the present invention contains a ginseng polysaccharide and a green tea polysaccharide as active ingredients, and the ginseng polysaccharide and the green tea polysaccharide are extracted from processed ginseng and green tea utilizing a herbicide processing technology.

The ginseng (Panax ginseng CA Meyer) polysaccharide used in the present invention is a pectin-like substance having a molecular weight of 34,600, and contains about 60% of galacturonic acid. In addition, arabinose, rhamnose, glucose and And galactose constitute side chains. The ginseng used in the present invention is a plant belonging to the genus Ogawa and Ginseng. In the present invention, ginseng can be used without limitation in its kind and year-round, and both dried and non-dried ginseng can be used. In the present invention, ginseng can be used without limitation in the site, and specifically, roots of ginseng can be used.

The green tea polysaccharide used in the present invention is derived from green tea and is an acidic polysaccharide group, unlike a polysaccharide found in other plants, and is produced by combining sugar components and amino acids produced through photosynthesis. In the present invention, green tea can be used regardless of the type and the number of days of cultivation, and both dried and non-dried green tea can be used. In the present invention, the green tea can be used without limitation in the area, and specifically, the leaves of green tea can be used.

As used herein, the term " formulated " means a constraint technique that changes the original properties of a medicament by processing the medicament based on the oriental theory. Foje can include boiling, steaming, roasting, roasting, burning, or boiling for the purpose of reducing the toxicity of the medicinal product, preserving the medicinal properties of the medicinal product, improving the medicinal properties, and improving the convenience of taking the medicinal product. In addition, the foaming method includes, for example, frying the herbal medicine, frying the medicinal material with a certain amount of the liquid auxiliary material, grinding the auxiliary material into the drug tissue, (Vapor) method in which a liquid auxiliary material is added and mixed, heated in a suitable container, and steamed or steamed until a certain degree is reached.

In addition, the casting method is further subdivided according to the intensity of the fire and the type of the material to be used. Here are some examples. It is a method of frying medicinal materials until it reaches a level defined by culture (fire) or artificial fire (fire). The liquorice method is a method of roasting a medicinal substance using about 15% of the alcohol and the method of boiling the medicinal substance using about 15% of alcohol. Even in the same medicinal plant, the properties of the raw material and the sown material may not be the same or may differ from each other. In addition, the combined prescription of a combination of raw materials and mature materials can maximize the therapeutic effect by elevating the drug efficacy of each medicinal substance or by canceling the toxicity.

"Foaming treatment" in the present invention refers to a process of pre-treating ginseng or green tea prior to the preparation of the extract by the above-described "Foje" method. Through the foaming treatment, substances that were not present in ginseng or green tea are newly produced , The effect of enhancing the efficacy of the existing substance can be obtained.

A method known in the art can be used for the method of extracting the polysaccharide from the ginseng or green tea treated with fog, and the method is not particularly limited.

Specifically, an extract of "ginseng or green tea" which has been treated with water at 38 to 42 ° C is prepared, and then concentrated. After removing impurities with a filter, ethanol is added dropwise thereto and the mixture is dried with hot air to obtain a green tea polysaccharide Ginseng polysaccharide can be obtained.

The composition of the present invention may contain the ginseng polysaccharide and the green tea polysaccharide in an amount of 0.001 to 10% by weight based on the total weight of the composition, preferably the ginseng polysaccharide and the green tea polysaccharide in an amount of 0.005 to 9.5% 0.09 to 7.5 wt.%, 0.1 to 6.5 wt.%, 0.3 to 6 wt.%, 0.5 to 5.5 wt.%, Or 0.7 By weight to 5% by weight.

In the present invention, the ginseng polysaccharide and the green tea polysaccharide are mixed in a weight ratio of 0.1 to 10: 1. In order to maximize the synergistic effect of the combination of the two components, preferably, the ginseng polysaccharide and the green tea polysaccharide are mixed in a weight ratio of 0.5 to 9.5: 1, 1 to 9: 1, or 1.5 to 8.5: 1 .

The composition of the present invention can be used as a composition for preventing dandruff. It can purify the scalp by effectively discharging toxins accumulated in hair and scalp, inhibit proliferation and growth of dandruff and prevent scalp inflammation reaction, It has excellent antioxidant ability to inhibit the production and action, so it can calm and strengthen the scalp and provide the effect of strengthening the original defense.

The composition of the present invention can be used as a hair tonic composition, which promotes the transition of the resting hair cycle to the growing hair cycle, thereby promoting hair growth and preventing hair loss.

The composition of the present invention can be used as a composition for external application for skin preventing anti-whiplash, which can significantly increase the expression of MITF in melanocytes, thereby inhibiting white moth and promoting gray hair induction.

The composition of the present invention can stabilize a sensitive, irritating or reactive scalp by reducing irritation such as itching and stinging of the scalp and alleviating and alleviating the scalp inflammation. In addition, hair condition can be improved healthily by providing a conditioning effect that makes rough and damaged hair soft and smooth.

The composition of the present invention may be formulated as an external preparation for skin, in particular as a cosmetic composition, and may be formulated containing a cosmetically or dermatologically acceptable medium or base. In addition, the composition of the present invention may be provided in any form suitable for topical application, for example, as a solution, an emulsion obtained by dispersing an oil phase in water phase, an emulsion obtained by dispersing water phase in water phase, a suspension, a solid, a gel, Pastes, foams, or aerosol compositions. Compositions of such formulations may be prepared according to conventional methods in the art.

In particular, the composition for external application for skin of the present invention is not particularly limited as a composition for hair or scalp. For example, hair tonic, hair nutritional lotion, scalp treatment, hair treatment, hair shampoo, hair rinse, Scalp hair combined treatment, and the like.

In addition, the composition according to the present invention may contain, in addition to the above-mentioned substances, other ingredients which can give a synergistic effect to the main effect, to the extent that the main effect is not impaired. The composition according to the present invention may further comprise a humectant, an emollient, an ultraviolet absorber, an antiseptic, a bactericide, an antioxidant, a pH adjuster, an organic or inorganic pigment, a perfume, a cold agent or a limiting agent. The compounding amount of the above components can be easily selected by a person skilled in the art within the range not impairing the object and effect of the present invention, and the amount thereof may be from 0.01 to 5% by weight, specifically from 0.01 to 3% by weight based on the total weight of the composition .

In addition, the composition of the present invention can be formulated as a pharmaceutical composition. When the composition according to the present invention is applied to medicines, it may be formulated into oral, parenteral or parenteral dosage forms in solid, semi-solid or liquid form by adding an inorganic or organic carrier compatible with the active ingredient used in the present invention , The pharmaceutical composition according to the present invention may be administered orally, parenterally, rectally, topically, transdermally, intravenously, intramuscularly, intraperitoneally, subcutaneously, and the like.

Examples of the formulations for oral administration include tablets, pills, granules, capsules, powders, fine granules, powders, emulsions, syrups and pellets. In addition, the formulations for parenteral administration include injections, drops, ointments, lotions, sprays, suspensions, emulsions, suppositories, and the like. The composition according to the present invention can be easily formulated into an active ingredient by carrying out the composition according to a conventional method. In this case, a surfactant, excipient, coloring agent, spice, preservative, stabilizer, buffer, suspending agent, have.

The dosage of the active ingredient of the pharmaceutical composition of the present invention will vary depending on the age, sex, body weight, pathological condition and severity of the subject to be treated, route of administration, or judgment of the prescriber. Determination of the appropriate dose based on these factors is well within the level of ordinary skill in the art and its daily dose is, for example, from 0.1 mg / kg / day to 100 mg / kg / day, more specifically from 5 mg / kg / day to 50 mg / / Day, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for illustrating the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

[Comparative Example 1-1] Preparation of Forsythia ginseng polysaccharide

The non-dried ginseng was put into a high pressure steam boiler and steamed at 95 to 100 ° C for about 3 to 4 hours under high pressure. Then, the steamed ginseng that had completed the above process steps was dried at 60 to 70 ° C in a hot air dryer. 1 kg of ginseng processed with forage preparation was pulverized with a blender, and 10 L of purified water was added thereto. The resulting mixture was stirred for 24 hours at 38 to 42 ° C in hot water, and then immersed at 15 ° C for 1 day. Thereafter, the residue and the filtrate were separated by filtration and centrifugation through a filter cloth, and the separated filtrate was concentrated under reduced pressure to 1/10 of the amount of purified water. Four times as much concentrated ethanol as the concentrated amount was added dropwise, and hot air drying was conducted to obtain 70 g of the ginseng polysaccharide produced by the foojyeong processing technique.

[Comparative Example 1-2] Preparation of fugitive green tea polysaccharide

70 g of a green tea polysaccharide processed by the same method as in Comparative Example 1-1 was obtained, except that the green tea leaves were used in the first processed (roughening process).

[Example 1-1] Preparation of booster ginseng and green tea polysaccharide (mixed at a weight ratio of 1: 3)

25% by weight of non-dried ginseng and 75% by weight of primary processed green tea leaves (75% by weight) were added to a high-pressure tiller and heated at 95 to 100 ° C for about 3 to 4 hours under high pressure. The dried ginseng was dried in a hot air dryer at a temperature of 60 to 70 ° C. 1 kg of a mixture of ginseng and green tea processed by forging process was pulverized with a blender, and 10 L of purified water was added thereto. The mixture was stirred at 38 to 42 캜 in hot water for 24 hours and then immersed at 15 캜 for 1 day. Thereafter, the residue and the filtrate were separated by filtration and centrifugation through a filter cloth, and the separated filtrate was concentrated under reduced pressure to 1/10 of the amount of purified water. Then, ethanol was added dropwise to a concentrated amount of ethanol, and the mixture was subjected to hot air drying to obtain 78 g of a mixture of ginseng polysaccharide and green tea polysaccharide,

[Example 1-2] Preparation of booster ginseng and green tea polysaccharide (mixed at a weight ratio of 1: 1)

A mixture of ginseng polysaccharide and green tea polysaccharide prepared by the same method as in Example 1-1 except that 50 wt% of non-dried ginseng and 50 wt% of green tea leaves of the first processed ≪ / RTI >

[Example 1-3] Preparation of booster ginseng and green tea polysaccharide (mixed at a weight ratio of 3: 1)

A mixture of ginseng polysaccharide and green tea polysaccharide prepared by the same method as in Example 1-1 except that 75% by weight of non-dried ginseng and 25% by weight of first-processed ≪ / RTI >

[Comparative Example 2-1] Preparation of polysaccharide of ginseng (salt)

Dry ginseng is mixed well with salt water (2 ~ 3%), sealed and left to be completely absorbed. Ginseng, which is completely absorbed in salt water, is put into a supergiant vessel at 100 ~ 180 ℃ and roasted for 10 minutes ~ 1 hour. I was dry. 10 L of purified water was added to the dried ginseng, refluxed three times, and then immersed at 15 캜 for one day. Thereafter, the residue and the filtrate were separated by filtration and centrifugation through a filter cloth, and the separated filtrate was concentrated under reduced pressure to 1/10 of the amount of purified water. The concentrated amount of ethanol was added dropwise thereto, and the mixture was hot-air dried to obtain 73 g of a ginseng-treated polysaccharide.

[Comparative Example 2-2] Production of green tea polysaccharide

76 g of a green tea polysaccharide obtained by the same procedure as in Comparative Example 2-1 was obtained, except that the green tea leaves were used in the primary treatment (smoothing process).

[Example 2-1] Production of ginseng and green tea polysaccharide (mixed at a weight ratio of 1: 3)

72 g of a ginseng polysaccharide and a green tea polysaccharide mixture were obtained in the same manner as in Comparative Example 2-1, except that 25% by weight of dried ginseng and 75% by weight of first-processed .

[Example 2-2] Preparation of ginseng and green tea polysaccharide (mixed at a weight ratio of 1: 1)

70 g of a ginseng polysaccharide and a green tea polysaccharide mixture were obtained by using the same method as in Comparative Example 2-1, except that 50% by weight of dried ginseng and 50% by weight of first-processed .

[Example 2-3] Preparation of ginseng and green tea polysaccharide (mixed at a weight ratio of 3: 1)

75 g of a ginseng polysaccharide and a green tea polysaccharide mixture were obtained by using the same method as in Comparative Example 2-1, except that 75% by weight of dried ginseng and 25% by weight of first-processed .

[Comparative Example 3-1] Production of polysaccharide of ginseng basil (roasted charcoal)

The dried ginseng was carbonized outside at 230 to 300 ° C. When it turned black, the heating was stopped. The mixture was cooled at room temperature. To 1 kg of ginseng thus obtained, 10 L of purified water was added, refluxed three times and then immersed at 15 ° C for 1 day. Thereafter, the filtrate and the filtrate were concentrated under reduced pressure to 1/10 of the amount of purified water through filtration of the filter cloth and centrifugation. Four times as much concentrated ethanol as the concentrated amount was added dropwise, and the mixture was hot-air dried to obtain 72 g of a ginseng polysaccharide which had been subjected to super-calcination.

[Comparative Example 3-2] Production of green tea chitosan polysaccharide

A green tea polysaccharide (72 g) was obtained by the same method as in Comparative Example 3-1, except that the green tea leaves were used in the primary treatment (smoothing process).

[Example 3-1] Production of ginseng and tea green tea polysaccharide (mixed at a weight ratio of 1: 3)

75 g of a ginseng polysaccharide and a green tea polysaccharide mixture were obtained by the same method as in Comparative Example 3-1, except that 25% by weight of dried ginseng and 75% by weight of first-processed (smoothing process) green tea leaves were used .

[Example 3-2] Production of ginseng and tea green tea polysaccharide (mixed at a weight ratio of 1: 1)

Using the same method as in Comparative Example 3-1, 71 g of a ginseng polysaccharide and a green tea polysaccharide mixture were obtained, except that 50% by weight of dried ginseng and 50% by weight of primary processed (roasted) green tea leaves were used .

[Example 3-3] Preparation of ginseng and tea green tea polysaccharide (mixed at a weight ratio of 3: 1)

70 g of a ginseng polysaccharide and a green tea polysaccharide mixture were obtained by the same method as in Comparative Example 3-1, except that 75% by weight of dried ginseng and 25% by weight of primary processed green tea leaves were used .

[Comparative Example 4-1] Preparation of ginseng horseradish polysaccharide

200-300 g of vinegar was sufficiently absorbed in dried ginseng and roasted at 100 ~ 160 ℃ for 10 minutes ~ 1 hour and dried in the shade. To 1 kg of the thus-obtained ginseng was added 10 L of purified water, refluxed three times, and then immersed at 15 캜 for 1 day. Thereafter, the filtrate and the filtrate were concentrated under reduced pressure to 1/10 of the amount of purified water through filtration of the filter cloth and centrifugation. Four times as much concentrated ethanol as the concentrated amount was added dropwise, and the mixture was hot-air dried to obtain 71 g of a gum polysaccharide prepared by processing.

[Comparative Example 4-2] Production of green tea hyaluronic acid polysaccharide

73 g of green tea polysaccharide prepared in the same manner as in Comparative Example 4-1 was obtained, except that green tea leaves were used in the first processed (roughening process).

[Example 4-1] Preparation of ginseng and tea green tea polysaccharide (mixed at a weight ratio of 1: 3)

Using the same method as in Comparative Example 4-1, 76 g of a mixture of green ginseng polysaccharide and green tea polysaccharide was obtained, except that 25% by weight of dry ginseng and 75% by weight of first-processed (smoothing process) green tea leaves were used .

[Example 4-2] Preparation of ginseng and tea green tea polysaccharide (mixed at a weight ratio of 1: 1)

74 g of a green ginseng polysaccharide mixture and a green tea polysaccharide mixture were obtained in the same manner as in Comparative Example 4-1, except that 50% by weight of dried ginseng and 50% by weight of primary processed green tea leaves were used .

[Example 4-3] Preparation of ginseng and tea green tea polysaccharide (mixed at a weight ratio of 3: 1)

Using the same method as in Comparative Example 4-1, 74 g of a mixture of green ginseng polysaccharide and green tea polysaccharide was obtained, except that 75% by weight of dried ginseng and 25% by weight of primary processed green tea leaves were used .

[Comparative Example 5-1] Preparation of ginseng saponin polysaccharide

Dried ginseng was soaked in a certain amount of sulfur, soaked in a steamer, steamed for 30 minutes to 2 hours, and then dried in the shade. To 1 kg of the thus-obtained ginseng was added 10 L of purified water, refluxed three times, and then immersed at 15 캜 for 1 day. Thereafter, the filtrate and the filtrate were concentrated under reduced pressure to 1/10 of the amount of purified water through filtration of the filter cloth and centrifugation. Four times as much concentrated ethanol as the concentrated amount was added dropwise, and the mixture was hot-air dried to obtain 77 g of a ginseng polysaccharide obtained by corn processing.

[Comparative Example 5-2] Preparation of green tea vernal polysaccharide

75 g of cornified green tea polysaccharide was obtained in the same manner as in Comparative Example 5-1, except that the green tea leaves were used for the first-time processing (smoothing process).

[Example 5-1] Production of ginseng and tea green tea polysaccharide (mixed at a weight ratio of 1: 3)

Using the same method as in Comparative Example 5-1, 74 g of a mixture of ginseng polysaccharide and green tea polysaccharide was obtained, except that 25% by weight of dry ginseng and 75% by weight of primary processed green tea leaves were used .

[Example 5-2] Preparation of ginseng and tea green tea polysaccharide (mixed at a weight ratio of 1: 1)

77 g of a ginseng polysaccharide mixture and a green tea polysaccharide mixture were obtained by using the same method as in Comparative Example 5-1 except that 50% by weight of dried ginseng and 50% by weight of first-processed .

[Example 5-3] Preparation of ginseng and tea green tea polysaccharide (mixed at a weight ratio of 3: 1)

77 g of a ginseng polysaccharide mixture and a green tea polysaccharide mixture were obtained by using the same method as in Comparative Example 5-1 except that 75% by weight of dried ginseng and 25% by weight of first-processed .

[Comparative Example 6-1] Preparation of ginseng fermented polysaccharide

Fresh ginger was crushed, and then double water was added to the mixture. The mixture was squeezed and squeezed. Ginger juice is prepared by repeating 2 ~ 3 times to prepare ginger juice. Spread ginger juice of 10 ~ 15% of ginseng with respect to the weight of ginseng in dried ginseng, and then, stirring ginger juice, it is put into a container at 100 ~ 180 캜 for 10 ~ Then I dried it in the shade. To 1 kg of the thus-obtained ginseng was added 10 L of purified water, refluxed three times, and then immersed at 15 캜 for 1 day. Thereafter, the filtrate and the filtrate were concentrated under reduced pressure to 1/10 of the amount of purified water through filtration of the filter cloth and centrifugation. Four times as much ethanol as the concentrated amount was added dropwise, and the mixture was hot-air dried to obtain 69 g of a strong-processed ginseng polysaccharide.

[Comparative Example 6-2] Preparation of green tea powder polysaccharide

73 g of a green tea polysaccharide prepared by the same method as in Comparative Example 6-1 was obtained, except that green tea leaves were used in the first processed (roughening process).

[Example 6-1] Preparation of ginseng and green tea strong polysaccharide (mixed at a weight ratio of 1: 3)

71 g of a mixture of a ginseng polysaccharide and a green tea polysaccharide was prepared by using the same method as in Comparative Example 6-1, except that 25% by weight of ginseng and 75% by weight of the first processed (roasted) green tea leaves were used.

[Example 6-2] Preparation of ginseng and green tea strong polysaccharide (mixed at a weight ratio of 1: 1)

Ginseng polysaccharide and 70 g of a green tea polysaccharide mixture were obtained by using the same method as in Comparative Example 6-1, except that 50% by weight of ginseng and 50% by weight of green tea leaves of the first processed (roasted) were used.

[Example 6-3] Preparation of ginseng and green tea strong polysaccharide (mixed at a weight ratio of 3: 1)

Ginseng polysaccharide and 70 g of a green tea polysaccharide mixture were obtained by using the same method as in Comparative Example 6-1, except that 75% by weight of ginseng and 25% by weight of primary processed (roasted) green tea leaves were used.

[Comparative Example 7-1] Production of ginseng millet (polysaccharide)

The dried ginseng was thoroughly absorbed in ginseng at 20 ~ 30% of the weight of ginseng, roasted at 100 ~ 160 ℃ for 10 minutes ~ 1 hour and dried in the shade. To 1 kg of the thus-obtained ginseng was added 10 L of purified water, refluxed three times, and then immersed at 15 캜 for 1 day. Thereafter, the filtrate and the filtrate were concentrated under reduced pressure to 1/10 of the amount of purified water through filtration of the filter cloth and centrifugation. To this was added a concentrated amount of ethanol in an amount of four times, followed by hot air drying to obtain 75 g of a milled ginseng polysaccharide.

[Comparative Example 7-2] Production of green tea pollen polysaccharide

76 g of milled green tea polysaccharide was obtained in the same manner as in Comparative Example 7-1, except that the green tea leaves were used in the first processed (roughening process).

[Example 7-1] Production of ginseng and tea green tea polysaccharide (mixed at a weight ratio of 1: 3)

Using the same method as in Comparative Example 7-1, 74 g of a mixture of ginseng polysaccharide and green tea polysaccharide was obtained, except that 25% by weight of dry ginseng and 75% by weight of first-processed (smoothing process) green tea leaves were used .

[Example 7-2] Preparation of ginseng and tea green tea polysaccharide (mixed at a weight ratio of 1: 1)

75 g of a mixture of milled ginseng polysaccharide and green tea polysaccharide was obtained in the same manner as in Comparative Example 7-1, except that 50% by weight of dried ginseng and 50% by weight of primary processed green tea leaves were used .

[Example 7-3] Preparation of ginseng and green tea pollen polysaccharide (mixed at a weight ratio of 3: 1)

Using the same method as in Comparative Example 7-1, 74 g of a mixture of ginseng polysaccharide and green tea polysaccharide was obtained, except that 75% by weight of dry ginseng and 25% by weight of primary processed (roasted) green tea leaves were used .

[Comparative Example 8-1] Preparation of polysaccharide extracted from ginseng product

1 kg of dried ginseng was pulverized with a blender, 10 L of purified water was added thereto, and the mixture was stirred for 24 hours at 38 to 42 ° C in hot water, and then immersed at 15 ° C for 1 day. Thereafter, the residue and the filtrate were separated by filtration and centrifugation through a filter cloth, and the separated filtrate was concentrated under reduced pressure to 1/10 of the amount of purified water. Four times as much ethanol as the concentrated amount was added dropwise, and the mixture was hot-air dried to obtain 65 g of ginseng polysaccharide.

[Comparative Example 8-2] Preparation of polysaccharide extracted from green tea product

72 g of green tea polysaccharide was obtained in the same manner as in Comparative Example 8-1, except that 1 kg of the first-processed green tea leaves were used.

[Comparative Example 8-3] Preparation of a polysaccharide mixture extracted from ginseng and green tea products (mixed at a weight ratio of 1: 3)

The ginseng polysaccharide prepared in Comparative Example 8-1 and the green tea polysaccharide prepared in Comparative Example 8-2 were simply mixed at a ratio of 1: 3 to prepare a mixture of ginseng polysaccharide and green tea polysaccharide.

[Comparative Example 8-4] Preparation of a polysaccharide mixture extracted from ginseng and green tea products (mixed at a weight ratio of 1: 1)

The mixture of ginseng polysaccharide and green tea polysaccharide prepared in Comparative Example 8-1 and Comparative Example 8-2 was simply mixed at a ratio of 1: 1 to prepare a mixture of ginseng polysaccharide and green tea polysaccharide.

[Comparative Example 8-5] Preparation of a polysaccharide mixture extracted from ginseng and green tea products (mixed at a weight ratio of 3: 1)

The mixture of ginseng polysaccharide and green tea polysaccharide prepared in Comparative Example 8-1 and Comparative Example 8-2 was simply mixed at a ratio of 3: 1 to prepare a mixture of ginseng polysaccharide and green tea polysaccharide.

Reference Example Preparation of Formulation Example and Comparative Formulation Example

Shampoos were prepared with the compositions of Tables 1 to 5 below. Specifically, the surfactant and ethylene glycol distearate were added to purified water, heated to 80 DEG C to dissolve uniformly, and then gradually cooled to 40 DEG C with stirring. To the mixture were added the active ingredient according to the present invention and a preservative, A viscosity adjusting agent, a perfume, and a hair conditioning agent were mixed and stirred, followed by cooling to room temperature.

ingredient Formulation Example 1-1 1-2 1-3 2-1 2-2 2-3 3-1 3-2 3-3 Ammonium lauryl sulfate 10 10 10 10 10 10 10 10 10 Polyoxyethylene lauryl ammonium sulfate 5 5 5 5 5 5 5 5 5 Cocoamidopropyl betaine 2 2 2 2 2 2 2 2 2 Ethylene glycol distearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Cocoyl monoethanolamide 0.8 0.8 0.8 0.8 0.8 0.8 0.8 0.8 0.8 Example 1-1 5.0 - - - - - - - - Examples 1-2 - 5.0 - - - - - - - Example 1-3 - - 5.0 - - - - - - Example 2-1 - - - 5.0 - - - - - Example 2-2 - - - - 5.0 - - - - Example 2-3 - - - - - 5.0 - - - Example 3-1 - - - - - - 5.0 - - Example 3-2 - - - - - - - 5.0 - Example 3-3 - - - - - - - - 5.0 Polyquaternium-10 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Blue No. 1 0.0002 0.0002 0.0002 0.0002 0.0002 0.0002 0.0002 0.0002 0.0002 Yellow No. 4 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 Methyl paraben 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Spices 0.8 0.8 0.8 0.8 0.8 0.8 0.8 0.8 0.8 Citric acid 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Dimethicone 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100

ingredient Formulation Example 4-1 4-2 4-3 5-1 5-2 5-3 6-1 6-2 6-3 Ammonium lauryl sulfate 10 10 10 10 10 10 10 10 10 Polyoxyethylene lauryl ammonium sulfate 5 5 5 5 5 5 5 5 5 Cocoamidopropyl betaine 2 2 2 2 2 2 2 2 2 Ethylene glycol distearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Cocoyl monoethanolamide 0.8 0.8 0.8 0.8 0.8 0.8 0.8 0.8 0.8 Example 4-1 5.0 - - - - - - - - Example 4-2 - 5.0 - - - - - - - Example 4-3 - - 5.0 - - - - - - Example 5-1 - - - 5.0 - - - - - Example 5-2 - - - - 5.0 - - - - Example 5-3 - - - - - 5.0 - - - Example 6-1 - - - - - - 5.0 - - Example 6-2 - - - - - - - 5.0 - Example 6-3 - - - - - - - - 5.0 Polyquaternium-10 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Blue No. 1 0.0002 0.0002 0.0002 0.0002 0.0002 0.0002 0.0002 0.0002 0.0002 Yellow No. 4 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 Methyl paraben 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Spices 0.8 0.8 0.8 0.8 0.8 0.8 0.8 0.8 0.8 Citric acid 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Dimethicone 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100

ingredient Formulation Example Comparative Formulation Example 7-1 7-2 7-3 1-1 1-2 2-1 2-2 3-1 3-2 Ammonium lauryl sulfate 10 10 10 10 10 10 10 10 10 Polyoxyethylene lauryl ammonium sulfate 5 5 5 5 5 5 5 5 5 Cocoamidopropyl betaine 2 2 2 2 2 2 2 2 2 Ethylene glycol distearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Cocoyl monoethanolamide 0.8 0.8 0.8 0.8 0.8 0.8 0.8 0.8 0.8 Example 7-1 5.0 - - - - - - - - Example 7-2 - 5.0 - - - - - - - Example 7-3 - - 5.0 - - - - - - Comparative Example 1-1 - - - 5.0 - - - - - Comparative Example 1-2 - - - - 5.0 - - - - Comparative Example 2-1 - - - - - 5.0 - - - Comparative Example 2-2 - - - - - - 5.0 - - Comparative Example 3-1 - - - - - - - 5.0 - Comparative Example 3-2 - - - - - - - - 5.0 Polyquaternium-10 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Blue No. 1 0.0002 0.0002 0.0002 0.0002 0.0002 0.0002 0.0002 0.0002 0.0002 Yellow No. 4 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 Methyl paraben 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Spices 0.8 0.8 0.8 0.8 0.8 0.8 0.8 0.8 0.8 Citric acid 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Dimethicone 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100

ingredient Comparative Formulation Example 4-1 4-2 5-1 5-2 6-1 6-2 7-1 7-2 Ammonium lauryl sulfate 10 10 10 10 10 10 10 10 Polyoxyethylene lauryl ammonium sulfate 5 5 5 5 5 5 5 5 Cocoamidopropyl betaine 2 2 2 2 2 2 2 2 Ethylene glycol distearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Cocoyl monoethanolamide 0.8 0.8 0.8 0.8 0.8 0.8 0.8 0.8 Comparative Example 4-1 5.0 - - - - - - - Comparative Example 4-2 - 5.0 - - - - - - Comparative Example 5-1 - - 5.0 - - - - - Comparative Example 5-2 - - - 5.0 - - - - Comparative Example 6-1 - - - - 5.0 - - - Comparative Example 6-2 - - - - - 5.0 - - Comparative Example 7-1 - - - - - - 5.0 - Comparative Example 7-2 - - - - - - - 5.0 Polyquaternium-10 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Blue No. 1 0.0002 0.0002 0.0002 0.0002 0.0002 0.0002 0.0002 0.0002 Yellow No. 4 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 Methyl paraben 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Spices 0.8 0.8 0.8 0.8 0.8 0.8 0.8 0.8 Citric acid 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Dimethicone 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 water to 100 to 100 to 100 to 100 to 100 to 100 to 100 to 100

ingredient Comparative Formulation Example 8-1 8-2 8-3 8-4 8-5 9 Ammonium lauryl sulfate 10 10 10 10 10 10 Polyoxyethylene lauryl ammonium sulfate 5 5 5 5 5 5 Cocoamidopropyl betaine 2 2 2 2 2 2 Ethylene glycol distearate 1.5 1.5 1.5 1.5 1.5 1.5 Cocoyl monoethanolamide 0.8 0.8 0.8 0.8 0.8 0.8 Comparative Example 8-1 5.0 - - - - - Comparative Example 8-2 - 5.0 - - - - Comparative Example 8-3 - - 5.0 - - - Comparative Example 8-4 - - - 5.0 - - Comparative Example 8-5 - - - - 5.0 - Polyquaternium-10 0.2 0.2 0.2 0.2 0.2 0.2 Blue No. 1 0.0002 0.0002 0.0002 0.0002 0.0002 0.0002 Yellow No. 4 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 Methyl paraben 0.1 0.1 0.1 0.1 0.1 0.1 Spices 0.8 0.8 0.8 0.8 0.8 0.8 Citric acid 0.1 0.1 0.1 0.1 0.1 0.1 Dimethicone 1.0 1.0 1.0 1.0 1.0 1.0 water to 100 to 100 to 100 to 100 to 100 to 100

[Test Example 1] Dandruff reduction effect test

410 males aged 19 to 35 years with a relatively large number of dandruff were selected and divided into 41 groups of 10, and the shampoos of Formulation Examples 1-1 to 7-3 and Comparative Formulation Examples 1-1 to 9 were applied for 1 month And the dysrhythmia reduction rate was measured.

Before the start of the test, the dandruff was sampled with normal shampoo, and the dandruff accumulated for 2 days after shaking was collected. The dandruff weight was sampled every two days with each shampoo. Respectively. The accumulated dandruff was collected directly from the scalp using a vacuum suction device, and the dandruff reduction rate was calculated according to the following equation (1). The results are shown in Table 6 below.

Dandruff reduction rate (%) Formulation Example 1-1 53.5 Formulation Example 1-2 52.4 Formulation Example 1-3 52.3 Comparative Formulation Example 1-1 36.9 Comparative Formulation Example 1-2 36.6 Formulation Example 2-1 49.7 Formulation Example 2-2 49.4 Formulation Example 2-3 49.5 Comparative Formulation Example 2-1 34.8 Comparative Formulation Example 2-2 33.9 Formulation Example 3-1 47.5 Formulation Example 3-2 46.0 Formulation Example 3-3 45.8 Comparative Formulation Example 3-1 35.4 Comparative Formulation Example 3-2 34.0 Formulation Example 4-1 51.9 Formulation Example 4-2 50.7 Formulation Example 4-3 50.1 Comparative Formulation Example 4-1 36.2 Comparative Formulation Example 4-2 35.4 Formulation Example 5-1 50.7 Formulation Example 5-2 49.9 Formulation Example 5-3 48.2 Comparative Formulation Example 5-1 35.7 Comparative Formulation Example 5-2 34.2 Formulation Example 6-1 49.3 Formulation Example 6-2 48.8 Formulation Example 6-3 49.0 Comparative Formulation Example 6-1 35.2 Comparative Formulation Example 6-2 34.9 Formulation Example 7-1 50.2 Formulation Example 7-2 50.4 Formulation Example 7-3 48.9 Comparative Formulation Example 7-1 35.4 Comparative Formulation Example 7-2 35.8 Comparative Formulation Example 8-1 30.8 Comparative Formulation Example 8-2 29.8 Comparative Formulation Example 8-3 34.5 Comparative Formulation Examples 8-4 34.2 Comparative Formulation Examples 8-5 32.9 Comparative Formulation Example 9 2.3

As can be seen from the above Table 6, it can be seen that the use of polysaccharide extracted from green ginseng or green tea, which is more fungicide treated than general ginseng and green tea polysaccharide, is more effective in preventing dandruff. In addition, the effectiveness of the mixture of ginseng polysaccharide and green tea polysaccharide was higher than that of the case of using ginseng polysaccharide and green tea polysaccharide, respectively. Especially, when the polysaccharides extracted from forage-treated ginseng and green tea were mixed, have.

[Test Example 2] Test for preventing the itchiness of the scalp

Forty-one men and women aged 25 to 45 years with severe scalp itching were selected and divided into 41 groups of 10, and the shampoos of Formulation Examples 1-1 to 7-3 and Comparative Formulation Examples 1-1 to 9 were divided into groups of 3 The results are shown in Table 7 below. ≪ tb >< TABLE >

[Evaluation standard]

Very good - 5 points

Excellent - 4 points

Usually - 3 points

Poor - 2 points

Very bad - 1 point

score Formulation Example 1-1 4.1 Formulation Example 1-2 4.0 Formulation Example 1-3 4.0 Comparative Formulation Example 1-1 3.7 Comparative Formulation Example 1-2 3.7 Formulation Example 2-1 3.8 Formulation Example 2-2 3.7 Formulation Example 2-3 3.6 Comparative Formulation Example 2-1 3.4 Comparative Formulation Example 2-2 3.3 Formulation Example 3-1 3.8 Formulation Example 3-2 3.8 Formulation Example 3-3 3.7 Comparative Formulation Example 3-1 3.4 Comparative Formulation Example 3-2 3.4 Formulation Example 4-1 4.0 Formulation Example 4-2 3.9 Formulation Example 4-3 4.0 Comparative Formulation Example 4-1 3.6 Comparative Formulation Example 4-2 3.5 Formulation Example 5-1 4.0 Formulation Example 5-2 3.9 Formulation Example 5-3 3.7 Comparative Formulation Example 5-1 3.5 Comparative Formulation Example 5-2 3.3 Formulation Example 6-1 3.8 Formulation Example 6-2 3.6 Formulation Example 6-3 3.5 Comparative Formulation Example 6-1 3.3 Comparative Formulation Example 6-2 3.2 Formulation Example 7-1 4.0 Formulation Example 7-2 3.8 Formulation Example 7-3 3.7 Comparative Formulation Example 7-1 3.4 Comparative Formulation Example 7-2 3.4 Comparative Formulation Example 8-1 3.1 Comparative Formulation Example 8-2 2.9 Comparative Formulation Example 8-3 3.4 Comparative Formulation Examples 8-4 3.5 Comparative Formulation Examples 8-5 3.4 Comparative Formulation Example 9 2.1

As can be seen from the above Table 7, it can be seen that the use of polysaccharide extracted from ginseng or green tea treated with forage treatment is superior to the effect of using common ginseng or green tea polysaccharide to prevent scalp itching. In addition, it was found that the effect of the mixture of ginseng polysaccharide and green tea polysaccharide was increased when they were mixed with each other. Especially, when polysaccharide extracted from forged ginseng and green tea were mixed, it is possible to prevent scalp itching more effectively .

[Test Example 3] Effect of proliferation of dermal papilla cells

The keratin proteins that make up the hair are produced in hair follicle keratinocyte, which keratinocytes differentiate from the dermal papilla cells. In order to evaluate the activity of the dermal papilla cells of the present composition, DP6 (rat immortalized dermal papilla cell) cell line was used in the present invention (Wendy Filsell, Journal of Cell Science 107, 1761-1772 (1994)). This dermal papilla cell line was obtained by microdissection in the hair roots of male PVG rat beard and cultured in DMEM (Dulbecco's modified Eagle's medium, Gibco BRL, Gaithersburg, MD, USA) containing 10% FBS (Fetal bovine serum) USA) for 24 hours in an incubator maintained at 37 ° C in 5% CO ₂ . DP6 was added to a 96-well plate and cultured in a 37 ° C incubator for 24 hours. Then, the polysaccharide or polysaccharide mixture of each of Examples 1-1 to 7-3 and Comparative Examples 1-1 to 8-5 was treated with 5 ppm, 10 ppm and 20 ppm Lt; / RTI > After 24 hours of drug treatment, cell proliferation was measured using WST-1 kit (Roche). The results are shown in Table 8 below.

division Cell proliferation (%) Untreated Control (Control) 100 Example 1-1 5 ppm 114 10ppm 119 20 ppm 133 Examples 1-2 5 ppm 115 10ppm 121 20 ppm 134 Example 1-3 5 ppm 114 10ppm 120 20 ppm 132 Comparative Example 1-1 5 ppm 112 10ppm 116 20 ppm 128 Comparative Example 1-2 5 ppm 113 10ppm 119 20 ppm 129 Example 2-1 5 ppm 114 10ppm 120 20 ppm 132 Example 2-2 5 ppm 114 10ppm 121 20 ppm 133 Example 2-3 5 ppm 115 10ppm 122 20 ppm 134 Comparative Example 2-1 5 ppm 113 10ppm 117 20 ppm 128 Comparative Example 2-2 5 ppm 112 10ppm 117 20 ppm 129 Example 3-1 5 ppm 115 10ppm 121 20 ppm 135 Example 3-2 5 ppm 115 10ppm 122 20 ppm 136 Example 3-3 5 ppm 114 10ppm 120 20 ppm 133 Comparative Example 3-1 5 ppm 113 10ppm 118 20 ppm 130 Comparative Example 3-2 5 ppm 113 10ppm 119 20 ppm 130 Example 4-1 5 ppm 114 10ppm 119 20 ppm 132 Example 4-2 5 ppm 115 10ppm 121 20 ppm 134 Example 4-3 5 ppm 115 10ppm 121 20 ppm 133 Comparative Example 4-1 5 ppm 112 10ppm 118 20 ppm 128 Comparative Example 4-2 5 ppm 113 10ppm 120 20 ppm 131 Example 5-1 5 ppm 115 10ppm 120 20 ppm 135 Example 5-2 5 ppm 115 10ppm 121 20 ppm 135 Example 5-3 5 ppm 116 10ppm 121 20 ppm 136 Comparative Example 5-1 5 ppm 113 10ppm 119 20 ppm 130 Comparative Example 5-2 5 ppm 114 10ppm 121 20 ppm 132 Example 6-1 5 ppm 115 10ppm 121 20 ppm 134 Example 6-2 5 ppm 116 10ppm 120 20 ppm 134 Example 6-3 5 ppm 115 10ppm 122 20 ppm 133 Comparative Example 6-1 5 ppm 113 10ppm 119 20 ppm 132 Comparative Example 6-2 5 ppm 113 10ppm 120 20 ppm 132 Example 7-1 5 ppm 114 10ppm 119 20 ppm 133 Example 7-2 5 ppm 114 10ppm 119 20 ppm 132 Example 7-3 5 ppm 115 10ppm 120 20 ppm 133 Comparative Example 7-1 5 ppm 112 10ppm 118 20 ppm 128 Comparative Example 7-2 5 ppm 113 10ppm 119 20 ppm 129 Comparative Example 8-1 5 ppm 108 10ppm 113 20 ppm 121 Comparative Example 8-2 5 ppm 110 10ppm 115 20 ppm 125 Comparative Example 8-3 5 ppm 113 10ppm 117 20 ppm 129 Comparative Example 8-4 5 ppm 112 10ppm 117 20 ppm 127 Comparative Example 8-5 5 ppm 112 10ppm 117 20 ppm 126

As can be seen from the above Table 8, the use of polysaccharide extracted from green tea or green tea, which has been subjected to forage treatment, is superior to that of general ginseng or green tea polysaccharide. In addition, when the ginseng polysaccharide and the green tea polysaccharide were used in combination, the cell proliferative ability was higher in the case of using them, and in particular, when the polysaccharide extracted from the folate-treated ginseng and green tea was mixed, the proliferation of the papilla cells was more effectively increased On the other hand, it can be confirmed that this is significantly increased depending on the concentration.

[Test Example 4] Evaluation of Potassium Ion Channel Activity Increase Effect

Minoxidil, a remedy for depilation, is known as a potential mitochondrial potassium ion channel opener (K _ATP channel opener) and is a representative drug used for the treatment of androgenetic alopecia. In order to evaluate the mechanism of minoxidil, cell proliferation was inhibited by treatment with tolbutamide (SIGMA AlDRICH, T0891) blocking K _ATP channel in fibroblasts constituting the dermis of the scalp, The restored test method was used.

To evaluate the function of this composition as a K _ATP channel opener, the fibroblast cell line NIH3T3 (mouse embryonic fibroblast cell line) cell line was used in the present invention. This cell line is a cell line obtained by natural immortalization of fibroblasts isolated from NIH Swiss mouse embryo by the 3T3 protocol. The cells were cultured in DMEM (Gibco BRL, Gaithersburg, MD, USA) containing 10% FBS for 24 hours in an incubator maintained at 37 ° C in 5% CO ₂ . NIH3T3 was added to a 96-well plate, cultured in a 37 ° C incubator for 24 hours, treated with 2.5 mM tolbutamide, and 10 minutes later, 10 μM of minoxidil, a positive control, and the cells of Examples 1-1 to 7- 1 to 8-5 polysaccharide or polysaccharide mixtures were treated at concentrations of 2.5 ppm, 5 ppm and 10 ppm, respectively, and cell proliferative activity was measured using WST-1 kit (Roche) after 48 hours of drug treatment. The results are shown in Table 9 below.

division Cell proliferation (%) Untreated Control (Control) 100 Minoxidil 132 Example 1-1 2.5 ppm 114 5 ppm 118 10ppm 127 Examples 1-2 2.5 ppm 113 5 ppm 117 10ppm 126 Example 1-3 2.5 ppm 113 5 ppm 118 10ppm 127 Comparative Example 1-1 2.5 ppm 110 5 ppm 114 10ppm 121 Comparative Example 1-2 2.5 ppm 109 5 ppm 113 10ppm 120 Example 2-1 2.5 ppm 112 5 ppm 117 10ppm 126 Example 2-2 2.5 ppm 112 5 ppm 116 10ppm 125 Example 2-3 2.5 ppm 112 5 ppm 117 10ppm 126 Comparative Example 2-1 2.5 ppm 108 5 ppm 113 10ppm 120 Comparative Example 2-2 2.5 ppm 108 5 ppm 114 10ppm 120 Example 3-1 2.5 ppm 114 5 ppm 118 10ppm 127 Example 3-2 2.5 ppm 114 5 ppm 117 10ppm 127 Example 3-3 2.5 ppm 113 5 ppm 117 10ppm 126 Comparative Example 3-1 2.5 ppm 109 5 ppm 113 10ppm 120 Comparative Example 3-2 2.5 ppm 110 5 ppm 114 10ppm 121 Example 4-1 2.5 ppm 112 5 ppm 117 10ppm 126 Example 4-2 2.5 ppm 113 5 ppm 117 10ppm 127 Example 4-3 2.5 ppm 113 5 ppm 118 10ppm 128 Comparative Example 4-1 2.5 ppm 109 5 ppm 113 10ppm 121 Comparative Example 4-2 2.5 ppm 109 5 ppm 115 10ppm 122 Example 5-1 2.5 ppm 113 5 ppm 117 10ppm 125 Example 5-2 2.5 ppm 113 5 ppm 116 10ppm 126 Example 5-3 2.5 ppm 113 5 ppm 117 10ppm 126 Comparative Example 5-1 2.5 ppm 108 5 ppm 112 10ppm 120 Comparative Example 5-2 2.5 ppm 108 5 ppm 113 10ppm 120 Example 6-1 2.5 ppm 113 5 ppm 118 10ppm 127 Example 6-2 2.5 ppm 114 5 ppm 118 10ppm 127 Example 6-3 2.5 ppm 114 5 ppm 119 10ppm 128 Comparative Example 6-1 2.5 ppm 109 5 ppm 114 10ppm 119 Comparative Example 6-2 2.5 ppm 110 5 ppm 115 10ppm 120 Example 7-1 2.5 ppm 113 5 ppm 117 10ppm 126 Example 7-2 2.5 ppm 113 5 ppm 117 10ppm 127 Example 7-3 2.5 ppm 114 5 ppm 118 10ppm 128 Comparative Example 7-1 2.5 ppm 108 5 ppm 113 10ppm 119 Comparative Example 7-2 2.5 ppm 110 5 ppm 114 10ppm 121 Comparative Example 8-1 2.5 ppm 110 5 ppm 114 10ppm 122 Comparative Example 8-2 2.5 ppm 111 5 ppm 115 10ppm 124 Comparative Example 8-3 2.5 ppm 111 5 ppm 116 10ppm 125 Comparative Example 8-4 2.5 ppm 106 5 ppm 111 10ppm 119 Comparative Example 8-5 2.5 ppm 106 5 ppm 112 10ppm 118

As can be seen from the above Table 9, the effect of using the polysaccharide extracted from the ginseng or green tea treated with the forage treatment is better than that of the normal ginseng or green tea polysaccharide. In addition, the cell proliferative capacity was higher when the ginseng polysaccharide and the green tea polysaccharide were mixed, respectively. In particular, when the polysaccharide extracted from the ginseng powder and the green tea mixed with the fossilized ginseng was used, the proliferation restoration of the fibroblast cell was more effective , While it increases significantly in a concentration-dependent manner. In addition, it was confirmed that the proliferation of fibroblast cells was restored to the minoxidil level when mixed with the polysaccharide extracted from the green tea and the fodder-treated ginseng.

[Test Example 5] Test for promoting melanin production

Melanocytes (melan-a) were added to a 24-well microtiter plate at 50,000 cells / well in RPMI medium supplemented with 5% fetal bovine serum, 100 IU penicillin G and 0.2 μM TPA Respectively. The following day, the cells were treated with the polysaccharide or polysaccharide mixture of Examples 1-1 to 7-3 and Comparative Examples 1-1 to 8-5 as test substances at a final concentration of 10 ppm or 50 ppm, and 0.1% DMSO , 100 μM IBMX as a positive control, and then cultured at 37 ° C. for 3 days. After incubation, the wells were washed with PBS, and 100 μl of 1N NaOH was added thereto, and melanin in the cells was dissolved. The absorbance of dissolved melanin was measured at 405 nm using a microplate reader. The results of comparing melanin production promoting effect of ginseng and green tea polysaccharide with the control group are shown in Table 10 below.

division Amount of melanin synthesis (%) DMSO (0.1%) 100 IBMX (100 [mu] M) 120 Example 1-1 10ppm 110 50 ppm 123 Examples 1-2 10ppm 111 50 ppm 125 Example 1-3 10ppm 111 50 ppm 126 Comparative Example 1-1 10ppm 107 50 ppm 118 Comparative Example 1-2 10ppm 107 50 ppm 119 Example 2-1 10ppm 112 50 ppm 124 Example 2-2 10ppm 112 50 ppm 125 Example 2-3 10ppm 113 50 ppm 125 Comparative Example 2-1 10ppm 109 50 ppm 119 Comparative Example 2-2 10ppm 110 50 ppm 121 Example 3-1 10ppm 112 50 ppm 126 Example 3-2 10ppm 112 50 ppm 127 Example 3-3 10ppm 112 50 ppm 125 Comparative Example 3-1 10ppm 110 50 ppm 120 Comparative Example 3-2 10ppm 110 50 ppm 119 Example 4-1 10ppm 112 50 ppm 126 Example 4-2 10ppm 113 50 ppm 128 Example 4-3 10ppm 113 50 ppm 126 Comparative Example 4-1 10ppm 109 50 ppm 118 Comparative Example 4-2 10ppm 109 50 ppm 119 Example 5-1 10ppm 112 50 ppm 124 Example 5-2 10ppm 112 50 ppm 125 Example 5-3 10ppm 113 50 ppm 127 Comparative Example 5-1 10ppm 109 50 ppm 117 Comparative Example 5-2 10ppm 110 50 ppm 120 Example 6-1 10ppm 111 50 ppm 124 Example 6-2 10ppm 111 50 ppm 126 Example 6-3 10ppm 113 50 ppm 128 Comparative Example 6-1 10ppm 108 50 ppm 119 Comparative Example 6-2 10ppm 109 50 ppm 119 Example 7-1 10ppm 113 50 ppm 126 Example 7-2 10ppm 113 50 ppm 125 Example 7-3 10ppm 113 50 ppm 125 Comparative Example 7-1 10ppm 111 50 ppm 120 Comparative Example 7-2 10ppm 109 50 ppm 118 Comparative Example 8-1 10ppm 105 50 ppm 117 Comparative Example 8-2 10ppm 106 50 ppm 119 Comparative Example 8-3 10ppm 109 50 ppm 121 Comparative Example 8-4 10ppm 110 50 ppm 122 Comparative Example 8-5 10ppm 111 50 ppm 122

Table 10 shows that the ginseng polysaccharide and the green tea polysaccharide promote the melanin synthesis of melanocytes and the melanin production is remarkably increased when mixed with polysaccharides extracted from forage-treated ginseng and green tea to promote excellent melanin production Effect.

Test Example 6: Promotion of MITF and tyrosinase expression in melanocytes

The cells were divided into six wells (6-well microtiter plate) to a concentration of 500,000 cells / well using a 501mel cell line. DMSO 0.1% as a negative control, IBMX 100 μM as a positive control, 1-1 to 7-3 and Comparative Examples 1-1 to 8-5 were treated at 10 ppm and cultured at 37 ° C for 24 hours, 48 hours and 72 hours to obtain proteins. The proteins thus obtained were subjected to western blotting using MITF and tyrosinase antibodies. Protein extraction and Western blotting were routinely performed by standard methods used by those skilled in the art. After Western blotting, the result was compared with the negative control group of 100, and the results are shown in Tables 11 and 12 below.

Western blot using MITF antibody division 24 hours 48 hours 72 hours IBMX 119 97 212 Example 1-1 86 118 156 Examples 1-2 86 115 152 Example 1-3 84 112 150 Comparative Example 1-1 79 112 144 Comparative Example 1-2 77 109 142 Example 2-1 87 117 158 Example 2-2 86 115 153 Example 2-3 86 115 154 Comparative Example 2-1 78 110 145 Comparative Example 2-2 80 112 148 Example 3-1 88 120 158 Example 3-2 87 118 156 Example 3-3 84 112 151 Comparative Example 3-1 81 114 148 Comparative Example 3-2 78 109 145 Example 4-1 87 118 157 Example 4-2 87 116 155 Example 4-3 85 113 152 Comparative Example 4-1 80 112 146 Comparative Example 4-2 79 110 144 Example 5-1 87 117 156 Example 5-2 86 115 155 Example 5-3 84 112 150 Comparative Example 5-1 78 108 145 Comparative Example 5-2 79 110 147 Example 6-1 87 119 159 Example 6-2 85 116 155 Example 6-3 83 112 151 Comparative Example 6-1 80 111 145 Comparative Example 6-2 78 108 143 Example 7-1 86 119 158 Example 7-2 85 115 154 Example 7-3 85 116 154 Comparative Example 7-1 81 112 145 Comparative Example 7-2 80 111 144 Comparative Example 8-1 78 110 148 Comparative Example 8-2 76 106 142 Comparative Example 8-3 86 117 154 Comparative Example 8-4 84 114 150 Comparative Example 8-5 80 111 148

Western blot using tyrosinase antibody division 24 hours 48 hours 72 hours IBMX 155 147 287 Example 1-1 143 174 201 Examples 1-2 142 174 200 Example 1-3 140 170 195 Comparative Example 1-1 138 165 190 Comparative Example 1-2 136 164 188 Example 2-1 144 177 205 Example 2-2 142 174 198 Example 2-3 143 175 200 Comparative Example 2-1 139 167 192 Comparative Example 2-2 136 164 188 Example 3-1 142 174 198 Example 3-2 142 175 199 Example 3-3 143 175 201 Comparative Example 3-1 138 166 191 Comparative Example 3-2 138 166 193 Example 4-1 140 171 195 Example 4-2 141 173 196 Example 4-3 143 174 200 Comparative Example 4-1 137 163 190 Comparative Example 4-2 138 164 190 Example 5-1 141 172 194 Example 5-2 142 174 196 Example 5-3 143 174 199 Comparative Example 5-1 136 162 188 Comparative Example 5-2 139 166 190 Example 6-1 142 172 198 Example 6-2 141 172 196 Example 6-3 143 174 200 Comparative Example 6-1 137 165 191 Comparative Example 6-2 137 165 190 Example 7-1 142 173 196 Example 7-2 140 171 194 Example 7-3 143 176 202 Comparative Example 7-1 139 163 190 Comparative Example 7-2 137 165 189 Comparative Example 8-1 125 154 176 Comparative Example 8-2 127 157 184 Comparative Example 8-3 134 160 188 Comparative Example 8-4 135 162 191 Comparative Example 8-5 138 167 193

As shown in Tables 11 and 12, the ginseng polysaccharide and green tea polysaccharide increase the expression of MITF and tyrosinase protein in melanocytes. Especially, when mixed with polysaccharides extracted from forage-treated ginseng and green tea, the expression of MITF and tyrosinase protein increases It is possible to confirm that the effect of making the film is very excellent.

[Referential Example 2] Preparation of Formulation Examples 8-1 to 14-3 and Comparative Formulation Examples 10-1 to 18

The scalp essences were prepared according to the compositions of Tables 13 to 17 using the above-described Examples 1-1 to 7-3 and Comparative Examples 1-1 to 8-5. More specifically, the efficacy materials of Examples 1-1 to 7-3 and Comparative Examples 1-1 to 8-5, polyoxyethylene hydrogenated castor oil / POE (60) (Emalex HC-60 , Nihon Emulsion Co., Ltd.), salicylic acid 0.2%, ethanol 10%, distilled water, ammonium acryloyldimethyltaurate / Vpicopolymer (manufacturer: Aristoflex) and phenoxyethanol (PE) .

(Unit: wt%) ingredient Formulation Example 8-1 8-2 8-3 9-1 9-2 9-3 10-1 10-2 10-3 Polyoxyethylene hydrogenated castor oil / POE (60) 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Example 1-1 0.2 - - - - - - 0.2 - Examples 1-2 - 0.2 - - - - - - - Example 1-3 - - 0.2 - - - - - - Example 2-1 - - - 0.2 - - - - - Example 2-2 - - - - 0.2 - - - - Example 2-3 - - - - - 0.2 - - - Example 3-1 - - - - - - 0.2 - - Example 3-2 - - - - - - - 0.2 - Example 3-3 - - - - - - - - 0.2 Salicylic acid 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 ethanol 10 10 10 10 10 10 10 10 10 Distilled water 88.3 88.3 88.3 88.3 88.3 88.3 88.3 88.3 88.3 Ammonium acryloyldimethyltaurate / Vpicopolymer 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Phenoxyethanol 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3

(Unit: wt%) ingredient Formulation Example 11-1 11-2 11-3 12-1 12-2 12-3 13-1 13-2 13-3 Polyoxyethylene hydrogenated castor oil / POE (60) 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Example 4-1 0.2 - - - - - - 0.2 - Example 4-2 - 0.2 - - - - - - - Example 4-3 - - 0.2 - - - - - - Example 5-1 - - - 0.2 - - - - - Example 5-2 - - - - 0.2 - - - - Example 5-3 - - - - - 0.2 - - - Example 6-1 - - - - - - 0.2 - - Example 6-2 - - - - - - - 0.2 - Example 6-3 - - - - - - - - 0.2 Salicylic acid 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 ethanol 10 10 10 10 10 10 10 10 10 Distilled water 88.3 88.3 88.3 88.3 88.3 88.3 88.3 88.3 88.3 Ammonium acryloyldimethyltaurate / Vpicopolymer 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Phenoxyethanol 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3

(Unit: wt%) ingredient Formulation Example Comparative Formulation Example 14-1 14-2 14-3 10-1 10-2 11-1 11-2 12-1 12-2 Polyoxyethylene hydrogenated castor oil / POE (60) 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Example 7-1 0.2 - - - - - - 0.2 - Example 7-2 - 0.2 - - - - - - - Example 7-3 - - 0.2 - - - - - - Comparative Example 1-1 - - - 0.2 - - - - - Comparative Example 1-2 - - - - 0.2 - - - - Comparative Example 2-1 - - - - - 0.2 - - - Comparative Example 2-2 - - - - - - 0.2 - - Comparative Example 3-1 - - - - - - - 0.2 - Comparative Example 3-2 - - - - - - - - 0.2 Salicylic acid 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 ethanol 10 10 10 10 10 10 10 10 10 Distilled water 88.3 88.3 88.3 88.3 88.3 88.3 88.3 88.3 88.3 Ammonium acryloyldimethyltaurate / Vpicopolymer 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Phenoxyethanol 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3

(Unit: wt%) ingredient Comparative Formulation Example 13-1 13-2 14-1 14-2 15-1 15-2 16-1 16-2 Polyoxyethylene hydrogenated castor oil / POE (60) 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Comparative Example 4-1 0.2 - - - - - - 0.2 Comparative Example 4-2 - 0.2 - - - - - - Comparative Example 5-1 - - 0.2 - - - - - Comparative Example 5-2 - - - 0.2 - - - - Comparative Example 6-1 - - - - 0.2 - - - Comparative Example 6-2 - - - - - 0.2 - - Comparative Example 7-1 - - - - - - 0.2 - Comparative Example 7-2 - - - - - - - 0.2 Salicylic acid 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 ethanol 10 10 10 10 10 10 10 10 Distilled water 88.3 88.3 88.3 88.3 88.3 88.3 88.3 88.3 Ammonium acryloyldimethyltaurate / Vpicopolymer 0.5 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Phenoxyethanol 0.3 0.3 0.3 0.3 0.3 0.3 0.3 0.3

(Unit: wt%) ingredient Comparative Formulation Example 17-1 17-2 17-3 17-4 17-5 18 Polyoxyethylene hydrogenated castor oil / POE (60) 0.5 0.5 0.5 0.5 0.5 0.5 Comparative Example 8-1 0.2 - - - - - Comparative Example 8-2 - 0.2 - - - - Comparative Example 8-3 - - 0.2 - - - Comparative Example 8-4 - - - 0.2 - - Comparative Example 8-5 - - - - 0.2 - Salicylic acid 0.2 0.2 0.2 0.2 0.2 0.2 ethanol 10 10 10 10 10 10 Distilled water 88.3 88.3 88.3 88.3 88.3 88.3 Ammonium acryloyldimethyltaurate / Vpicopolymer 0.5 0.5 0.5 0.5 0.5 0.5 Phenoxyethanol 0.3 0.3 0.3 0.3 0.3 0.3

[Test Example 7] Skin irritation test

In order to confirm whether or not the skin irritation occurred when using the composition according to the present invention, 410 men and women aged 25 to 45 years who were relatively scarred with scalp itching were selected and divided into 41 groups of 10 persons. Formulations 8-1 to 14- 3 and Comparative Formulations 10-1 to 18 were used. Concretely, after washing the cheek piece, the water was wiped off and the sample was allowed to stand in a constant temperature and humidity room (temperature 24 ± 4 ° C., relative humidity 40 to 45%) for 15 minutes. The same amount of the scalp essence containing 0.2% of the efficacious substance was applied to the skin surface to the extent that did not allow the skin essence to flow therethrough. After 24 hours and 48 hours after the test substance was attached, the subject marked the degree of stimulation from 0 to 4 points (0 = no stimulation, 1 = very weak, 2 = weak, 3 = moderate, 4 = severe) (2 measurements in total), stinging, and burning sensation.

The number of subjects who complained of the above-mentioned stimulus for each time slot is shown in Table 18 below. The average value was calculated to derive the skin irritation as a mean score, and the results are shown in Table 18 below.

Test material name Test concentration 24hr 48hr Responses Judgment One 2 3 4 One 2 3 4 (n = 10) Formulation Example 8-1 0.20% One 0 0 0 0 0 0 0 One No stimulation Formulation Example 8-2 0.20% One 0 0 0 0 0 0 0 One No stimulation Formulation Example 8-3 0.20% One 0 0 0 0 0 0 0 One No stimulation Comparative Formulation Example 10-1 0.20% One 0 0 0 0 0 0 0 One No stimulation Comparative Formulation Example 10-2 0.20% One 0 0 0 0 0 0 0 One No stimulation Formulation Example 9-1 0.20% One 0 0 0 0 0 0 0 One No stimulation Formulation Example 9-2 0.20% One 0 0 0 0 0 0 0 One No stimulation Formulation Example 9-3 0.20% One 0 0 0 0 0 0 0 One No stimulation Comparative Formulation Example 11-1 0.20% One 0 0 0 0 0 0 0 One No stimulation Comparative Formulation Example 11-2 0.20% One 0 0 0 0 0 0 0 One No stimulation Formulation Example 10-1 0.20% One 0 0 0 0 0 0 0 One No stimulation Formulation Example 10-2 0.20% One 0 0 0 0 0 0 0 One No stimulation Formulation Example 10-3 0.20% One 0 0 0 0 0 0 0 One No stimulation Comparative Formulation Example 12-1 0.20% One 0 0 0 0 0 0 0 One No stimulation Comparative Formulation Example 12-2 0.20% One 0 0 0 0 0 0 0 One No stimulation Formulation Example 11-1 0.20% One 0 0 0 0 0 0 0 One No stimulation Formulation Example 11-2 0.20% One 0 0 0 0 0 0 0 One No stimulation Formulation Example 11-3 0.20% One 0 0 0 0 0 0 0 One No stimulation Comparative Formulation Example 13-1 0.20% One 0 0 0 0 0 0 0 One No stimulation Comparative Formulation Example 13-2 0.20% One 0 0 0 0 0 0 0 One No stimulation Formulation Example 12-1 0.20% One 0 0 0 0 0 0 0 One No stimulation Formulation Example 12-2 0.20% One 0 0 0 0 0 0 0 One No stimulation Formulation Example 12-3 0.20% One 0 0 0 0 0 0 0 One No stimulation Comparative Formulation Example 14-1 0.20% One 0 0 0 0 0 0 0 One No stimulation Comparative Formulation Example 14-2 0.20% One 0 0 0 0 0 0 0 One No stimulation Formulation Example 13-1 0.20% One 0 0 0 0 0 0 0 One No stimulation Formulation Example 13-2 0.20% One 0 0 0 0 0 0 0 One No stimulation Formulation Example 13-3 0.20% One 0 0 0 0 0 0 0 One No stimulation Comparative Formulation Example 15-1 0.20% One 0 0 0 0 0 0 0 One No stimulation Comparative Formulation Example 15-2 0.20% One 0 0 0 0 0 0 0 One No stimulation Formulation Example 14-1 0.20% One 0 0 0 0 0 0 0 One No stimulation Formulation Example 14-2 0.20% One 0 0 0 0 0 0 0 One No stimulation Formulation Example 14-3 0.20% One 0 0 0 0 0 0 0 One No stimulation Comparative Formulation Example 16-1 0.20% One 0 0 0 0 0 0 0 One No stimulation Comparative Formulation Example 16-2 0.20% One 0 0 0 0 0 0 0 One No stimulation Comparative Formulation Example 17-1 0.20% One 0 0 0 0 0 0 0 One No stimulation Comparative Formulation Example 17-2 0.20% One 0 0 0 0 0 0 0 One No stimulation Comparative Formulation Example 17-3 0.20% One 0 0 0 0 0 0 0 One No stimulation Comparative Formulation Example 17-4 0.20% One 0 0 0 0 0 0 0 One No stimulation Comparative Formulation Examples 17-5 0.20% One 0 0 0 0 0 0 0 One No stimulation Comparative Formulation Example 18 0.20% 0 0 0 0 One 0 0 0 One No stimulation

In the results of Table 18 above, the compositions containing the polysaccharide or polysaccharide mixture of Formulation Examples 8-1 to 14-3 and Comparative Formulation Examples 10-1 to 17-5 were obtained in the same manner as Comparative Formulation Example 18 containing no polysaccharide It was confirmed that there was no stimulation. Therefore, it can be seen that the composition according to the present invention is safe to the human body and has no irritation to the skin by using the polysaccharide derived from ginseng and green tea as an active ingredient, and has no side effects even when it is used for a long time.

[Reference Example 3] Preparation of Formulation Examples 15-1 to 21-3 and Comparative Formulation Examples 19-1 to 27

Using the above-mentioned Examples 1-1 to 7-3 and Comparative Examples 1-1 to 8-5, a scalp and hair combination treatment composition was prepared according to the compositions of Tables 19 to 23 below. The oil phase and water phase were separately prepared in a blender, the oil phase was gradually added to the water phase, emulsified at 3,000 rpm for 10 minutes using a homomixer, and cooled to room temperature to obtain Formulation Examples 15-1 to 21-3 and Comparative Formulation Examples 19-1 To 27 < / RTI > of scalp and hair combs were prepared.

Component (% by weight) Formulation Example 15-1 15-2 15-3 16-1 16-2 16-3 17-1 17-2 17-3 Purified water balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) Example 1-1 0.5 - - - - - - - - Examples 1-2 - 0.5 - - - - - - - Example 1-3 - - 0.5 - - - - - - Example 2-1 - - - 0.5 - - - - - Example 2-2 - - - - 0.5 - - - - Example 2-3 - - - - - 0.5 - - - Example 3-1 - - - - - - 0.5 - - Example 3-2 - - - - - - - 0.5 - Example 3-3 - - - - - - - - 0.5 Cetyltrimethylammonium chloride 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Cetyl alcohol 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Stearyl alcohol 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Preservatives, coloring and flavoring Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount

Component (% by weight) Formulation Example 18-1 18-2 18-3 19-1 19-2 19-3 20-1 20-2 20-3 Purified water balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) Example 4-1 0.5 - - - - - - - - Example 4-2 - 0.5 - - - - - - - Example 4-3 - - 0.5 - - - - - - Example 5-1 - - - 0.5 - - - - - Example 5-2 - - - - 0.5 - - - - Example 5-3 - - - - - 0.5 - - - Example 6-1 - - - - - - 0.5 - - Example 6-2 - - - - - - - 0.5 - Example 6-3 - - - - - - - - 0.5 Cetyltrimethylammonium chloride 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Cetyl alcohol 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Stearyl alcohol 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Preservatives, coloring and flavoring Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount

Component (% by weight) Formulation Example Comparative Formulation Example 21-1 21-2 21-3 19-1 19-2 20-1 20-2 21-1 21-2 Purified water balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) Example 7-1 0.5 - - - - - - - - Example 7-2 - 0.5 - - - - - - - Example 7-3 - - 0.5 - - - - - - Comparative Example 1-1 - - - 0.5 - - - - - Comparative Example 1-2 - - - - 0.5 - - - - Comparative Example 2-1 - - - - - 0.5 - - - Comparative Example 2-2 - - - - - - 0.5 - - Comparative Example 3-1 - - - - - - - 0.5 - Comparative Example 3-2 - - - - - - - - 0.5 Cetyltrimethylammonium chloride 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Cetyl alcohol 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Stearyl alcohol 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Preservatives, coloring and flavoring Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount

Component (% by weight) Comparative Formulation Example 22-1 22-2 23-1 23-2 24-1 24-2 25-1 25-2 Purified water balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) Comparative Example 4-1 0.5 - - - - - - - Comparative Example 4-2 - 0.5 - - - - - - Comparative Example 5-1 - - 0.5 - - - - - Comparative Example 5-2 - - - 0.5 - - - - Comparative Example 6-1 - - - - 0.5 - - - Comparative Example 6-2 - - - - - 0.5 - - Comparative Example 7-1 - - - - - - 0.5 - Comparative Example 7-2 - - - - - - - 0.5 Cetyltrimethylammonium chloride 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Cetyl alcohol 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Stearyl alcohol 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Preservatives, coloring and flavoring Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount

Component (% by weight) Comparative Formulation Example 26-1 26-2 26-3 26-4 26-5 27 Purified water balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) balance
(to 100) Comparative Example 8-1 0.5 - - - - - Comparative Example 8-2 - 0.5 - - - - Comparative Example 8-3 - - 0.5 - - - Comparative Example 8-4 - - - 0.5 - - Comparative Example 8-5 - - - - 0.5 - Cetyltrimethylammonium chloride 1.0 1.0 1.0 1.0 1.0 1.0 Cetyl alcohol 3.0 3.0 3.0 3.0 3.0 3.0 Stearyl alcohol 3.0 3.0 3.0 3.0 3.0 3.0 Preservatives, coloring and flavoring Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount

[Test Example 9]

Sensory tests were carried out on the scalp and hair-combined treatment compositions of Formulation Examples 15-1 to 21-3 and Comparative Formulation Examples 19-1 to 27 by the following method. Forty-one 410 professional evaluation personnel were divided into 41 groups of 10, and the scalp and hair-combing treatments of Formulation Examples 15-1 to 21-3 and Comparative Formulation Examples 19-1 to 27 were used for each group, A comparison test was performed on the items shown in Table 24, and the test was conducted with the lowest score being 1 and the highest score being 5. The test results are shown in Table 24 below.

Item Scalp itching Scalp stimulus Hair softness / smoothness Formulation Example 15-1 2.2 2.2 2.5 Formulation Example 15-2 2.1 2.0 2.6 Formulation Example 15-3 2.1 2.1 2.7 Comparative Formulation Example 19-1 2.5 2.4 2.3 Comparative Formulation Example 19-2 2.3 2.4 2.4 Formulation Example 16-1 2.1 2.2 2.7 Formulation Example 16-2 2.0 2.0 2.8 Formulation Example 16-3 2.0 2.0 2.9 Comparative Formulation Example 20-1 2.4 2.5 2.4 Comparative Formulation Example 20-2 2.2 2.6 2.7 Formulation Example 17-1 1.9 1.9 2.8 Formulation Example 17-2 1.9 2.0 2.7 Formulation Example 17-3 2.1 2.1 2.6 Comparative Formulation Example 21-1 2.2 2.3 2.5 Comparative Formulation Example 21-2 2.4 2.5 2.3 Formulation Example 18-1 2.0 2.1 2.6 Formulation Example 18-2 2.0 2.0 2.7 Formulation Example 18-3 2.1 2.1 2.7 Comparative Formulation Example 22-1 2.3 2.5 2.3 Comparative Formulation Example 22-2 2.5 2.6 2.2 Formulation Example 19-1 1.8 1.8 2.9 Formulation Example 19-2 1.9 2.0 2.7 Formulation Example 19-3 2.0 2.2 2.6 Comparative Formulation Example 23-1 2.4 2.5 2.3 Comparative Formulation Example 23-2 2.2 2.5 2.3 Formulation Example 20-1 2.1 2.1 2.9 Formulation Example 20-2 2.2 2.2 2.8 Formulation Example 20-3 2.1 2.2 2.9 Comparative Formulation Example 24-1 2.6 2.5 2.3 Comparative Formulation Example 24-2 2.4 2.4 2.4 Formulation Example 21-1 2.2 2.3 2.9 Formulation Example 21-2 2.3 2.5 2.8 Formulation Example 21-3 2.2 2.3 2.9 Comparative Formulation Example 25-1 2.4 2.6 2.6 Comparative Formulation Example 25-2 2.2 2.5 2.6 Comparative Formulation Example 26-1 3.3 3.1 2.1 Comparative Formulation Example 26-2 3.1 3.0 2.3 Comparative Formulation Example 26-3 3.0 2.9 2.2 Comparative Formulation Example 26-4 3.0 3.0 2.4 Comparative Formulation Examples 26-5 2.8 2.8 2.4 Comparative Formulation Example 27 4.1 3.8 1.5

Table 24 shows that Formulation Examples 15-1 to 21-3 containing ginseng or green tea polysaccharide and Comparative Formulation Examples 19-1 to 26-5 and Comparative Example 27 containing no polysaccharide were used , The itching of the scalp was reduced, the irritation was alleviated, and the hair condition was also improved. Particularly, the use of Formulation Examples 15-1 to 21-3 comprising a mixture of ginseng treated with fog and a polysaccharide extracted from green tea can be confirmed to be very excellent.

 [Formulation Example 1] Hair tonic

Hair tonics were prepared in a conventional manner according to the composition shown in Table 25 below.

ingredient Weight ratio (%) ethanol 50 menthol 0.02 glycerin 3 Salicylic acid 0.05 The polysaccharide mixture of Example 1-2 1.0 Spices and Pigments Proper amount Purified water Balance (to 100)

[Formulation Example 2] Hair lotion

Hair lotions were prepared according to the composition shown in Table 26 below in a conventional manner.

ingredient Weight ratio (%) Cetostearyl alcohol 2.0 EDTA 2Na 0.2 Hydroxyethyl cellulose 0.5 Mineral oil 5.0 The polysaccharide mixture of Example 1-2 1.0 antiseptic Proper amount Spices and Pigments Proper amount Purified water Balance (to 100)

[Formulation Example 3] Hair nutritional lotion

A hair nutritional lotion was prepared by a conventional method according to the composition shown in Table 27 below.

ingredient Weight ratio (%) Wax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.7 Mineral oil 10.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Triethanolamine 0.2 The polysaccharide mixture of Example 1-2 1.0 antiseptic Proper amount Spices and Pigments Proper amount Purified water Balance (to 100)

[Formulation Example 4] Hair shampoo

Hair shampoos were prepared in a conventional manner according to the composition shown in Table 28 below.

ingredient weight% Purified water Balance (To 100) The polysaccharide mixture of Example 1-2 1.0 Sodillauryl sulphite 36.0 Cocamidopropyl betaine 8.0 Palmitidine dimalate 2.0 Glycollosteralate 1.5 Polyquaternium 10 0.5 Saitric Acid 0.1 glycerin 2.0 Preservatives, coloring and flavoring Suitable amount

[Formulation Example 5] Hair rinse

The hair rinse was prepared according to a conventional method according to the composition shown in Table 29 below.

ingredient weight% Purified water Balance (To 100) The polysaccharide mixture of Example 1-2 1.0 Propylene glycol 2.0 Cetyltrimethylammonium chloride 1.0 Cetyl alcohol 3.0 Stearyl alcohol 3.0 Mineral oil 0.5 Saitric Acid 0.2 Polydimethylsiloxane 1.0 Preservatives, coloring and flavoring Suitable amount

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will readily appreciate that many modifications are possible in the exemplary embodiments without materially departing from the novel teachings and advantages of this invention. something to do. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (15)

Polysaccharides extracted from folate-treated ginseng; And a polysaccharide extracted from a green tea treated as an active ingredient. 2. The composition for external application for skin according to claim 1, wherein the polysaccharide extracted from the fomed ginseng and the polysaccharide extracted from the fomed green tea are contained in an amount of 0.001-10 wt% based on the total weight of the composition. The composition for external application for skin according to claim 1, wherein the polysaccharide extracted from the fomed ginseng and the polysaccharide extracted from the green tea are mixed at a ratio of 0.1 to 10: 1 by weight. The composition for external application for skin according to claim 1, wherein the composition is for preventing dandruff. The composition for external application for skin according to claim 1, wherein the composition is for hair growth. The composition for external application for skin according to claim 1, wherein the composition is for prevention of white hair. The composition for external application for skin according to claim 1, wherein the composition is for alleviating scalp irritation. The composition for external application for skin according to claim 1, wherein the composition is for improving hair softness. Polysaccharides extracted from folate-treated ginseng; And a polysaccharide extracted from a green tea treated with a fogging agent as an active ingredient. [Claim 11] The pharmaceutical composition according to claim 9, wherein the polysaccharide extracted from the fomed ginseng and the polysaccharide extracted from the green tea are contained in an amount of 0.001-10 wt% based on the total weight of the composition. [Claim 10] The pharmaceutical composition according to claim 9, wherein the polysaccharide extracted from the fungus-treated ginseng and the polysaccharide extracted from the green tea are mixed at a ratio of 0.1 to 10: 1 by weight. 10. The pharmaceutical composition according to claim 9, wherein the composition is for preventing dandruff. 10. The pharmaceutical composition of claim 9, wherein the composition is hair tonic. 10. The pharmaceutical composition according to claim 9, wherein said composition is for preventing white hair. 10. The pharmaceutical composition according to claim 9, wherein said composition is for alleviating scalp irritation.